Seaweed and Seaweed-Based Functional Metabolites as Potential Modulators of Growth, Immune and Antioxidant Responses, and Gut Microbiota in Fish

Seaweed, also known as macroalgae, represents a vast resource that can be categorized into three taxonomic groups: Rhodophyta (red), Chlorophyta (green), and Phaeophyceae (brown). They are a good source of essential nutrients such as proteins, minerals, vitamins, and omega-3 fatty acids. Seaweed also contains a wide range of functional metabolites, including polyphenols, polysaccharides, and pigments. This study comprehensively discusses seaweed and seaweed-derived metabolites and their potential as a functional feed ingredient in aquafeed for aquaculture production. Past research has discussed the nutritional role of seaweed in promoting the growth performance of fish, but their effects on immune response and gut health in fish have received considerably less attention in the published literature. Existing research, however, has demonstrated that dietary seaweed and seaweed-based metabolite supplementation positively impact the antioxidant status, disease resistance, and stress response in fish. Additionally, seaweed supplementation can promote the growth of beneficial bacteria and inhibit the proliferation of harmful bacteria, thereby improving gut health and nutrient absorption in fish. Nevertheless, an important balance remains between dietary seaweed inclusion level and the resultant metabolic alteration in fish. This review highlights the current state of knowledge and the associated importance of continued research endeavors regarding seaweed and seaweed-based functional metabolites as potential modulators of growth, immune and antioxidant response, and gut microbiota composition in fish.


Introduction
Aquaculture has become an essential source of seafood for the human population and contributes significantly to global food security.With the demand for nutritious seafood increasing due to population growth and declining wild fish stocks, aquaculture has helped bridge the gap between supply and demand [1].However, continually increasing aquafeed costs and disease outbreaks have become common challenges impacting the profitability and sustainability of this sector [2,3].As such, aquaculture researchers globally are looking for more affordable and health-promoting feed ingredients that can sustain fish growth as well as prevent disease in aquaculture production.In recent years, there has been a growing interest in utilizing various seaweeds, including red seaweed (e.g., Gracilaria, Porphyra), brown seaweed (e.g., Laminaria, Ascophyllum), and green seaweed (e.g., Ulva, formerly Enteromorpha), as potential sources of bioactive compounds and feed ingredients for inclusion in aquafeed given their favorable nutritional composition, environmental sustainability, and potential health-promoting factors for farmed fish [4].A growing number of studies have indicated that seaweed and seaweed-based functional metabolites improve serum immune and antioxidant status [5] and disease resistance [6] in fish when incorporated in aquafeed as a supplement.
Seaweed is a valuable source of protein, ranging from 11% to 32% in its composition (dry weight, DW), with an excellent combination of essential amino acids, soluble dietary fiber, minerals, and vitamins suitable for use in aquafeed formulations [4,7,8].However, the protein and its essential amino acid levels have been shown to be low and variable depending on species, growing conditions, season of harvest, water depth, salinity of water, processing methods, and geographical conditions [9].Previous research has shown that low inclusion levels (~5%) of seaweed or their bioactive metabolites in fish diets evoke positive effects on growth performance [10], while inclusion levels (~10%) in fish diets neither improve nor decrease growth performance in fish [11].Similarly, Soler-vila et al. [12] reported that up to 10% inclusion of red alga (Porphyra dioica) in a practical diet does not compromise growth, whilst an inclusion level of 15% reduced growth performance in rainbow trout (Oncorhynchus mykiss).However, the nutrient utilization efficiency of seaweed in aquafeed is dependent on the feeding habits of cultured species and the inclusion rate [13].For instance, in carnivorous species like European seabass (Dicentrarchus labrax), the optimum inclusion level of Ulva lactuca has been reported as being 5% of the diet [14], while in omnivorous species like Nile tilapia (Oreochromis niloticus), this seaweed species can constitute up to 20% of practical diets with no detrimental effects on growth performance [15].Nevertheless, seaweed is also a good source of omega-3 fatty acids, particularly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), in their fatty acid profile [16] in comparison with terrestrial plants.These fatty acids are essential for the metabolic activity of fish as well as maintaining the structural integrity and the fluidity and permeability of cellular membranes [17].
Recent research findings have revived interest in seaweed as a safe alternative to preventive and therapeutic drugs in farmed fish to reduce economic loss caused by infectious diseases [6].Seaweed has a wide range of bioactive substances, including polyphenols, pigments, essential fatty acids, vitamins, and amino acids [18], where seaweed polyphenols are reported to enhance the immune response and disease resistance in fish [19,20].Thanigaivel et al. [21] reported that dietary administration of total phenolic compounds (TPC) from Gracilaria foliifera (Rhodophyta) and Sargassum longifolium (Phaeophyceae) at inclusion levels ranging from 14. 71 mg/g GAE to 18.42 mg/g GAE (gallic acid equivalent) in Mozambique tilapia (Oreochromis mossambicus) increased percent survival against Aeromonas salmonicida infection.However, the existing literature mostly deals with seaweed and seaweed extract as aquafeed supplements for fish.There are currently very few studies on how seaweed-derived bioactive compounds can supplement aquafeed with the goal of improving fish health.Enhanced lysozyme and immune-related gene expression and improved stress response and disease resistance have also been reported in various fish fed seaweed-supplemented diets [6,22].However, the immunostimulatory properties of seaweed also depend on the level of inclusion in the diet.For example, Vazirzadeh et al. [22] reported a significant increase in lysozyme activity in rainbow trout when supplemented with 5% Gracilariopsis persica (Rhodophyta) in the diet, while 10% inclusion had no effect following an 83-day feeding trial.These findings suggest that seaweed provides health benefits when fed to fish at optimized levels and, therefore, has potential as a functional ingredient in fish feeds.Furthermore, seaweed may enhance fish gut health by altering gut microbiota, thus strengthening the gut barrier function.For instance, an abundance of beneficial bacteria such as Shewanella sp. has been reported when adding seaweed (Asparagopsis taxiformis (Rhodophyta) extract to feeds for Atlantic salmon (Salmo salar) [23].Also, augmented villus height and increased intestinal goblet cell numbers have been reported following algae (Spirulina platensis) supplementation in fish [24].
Despite many positive attributes, seaweed has seldom been researched as a source of functional metabolites in aquafeed for fish.The potential effects of dietary seaweed and

Seaweed Resources and Seaweed's Nutritional Composition
Seaweed can be broadly categorized into three main types, including brown, red, and green seaweed under the phylum groups of Phaeophyceae, Rhodophyta, and Chlorophyta, respectively.Within these phyla, there are a multitude of morphologies.Figure 1 depicts the various types of seaweeds.Seaweeds have evolved to either be attached to hard substrates, such as rocks, with the help of special root-like organs known as holdfasts, or be free-floating [25].As highlighted above, seaweeds are a good source of essential nutrients such as proteins, vitamins, minerals, and bioactive compounds.Notably, the composition may vary depending on the season, water depth, salinity, and geography.The groups of seaweed and their nutritional composition are discussed below.

Brown Seaweed
Brown seaweed, which includes the structurally complex forms of kelp, is the most common type of seaweed found in coastal areas around the world.Brown seaweed gets its name from its characteristic brown or olive-green color, which is due to the presence of a pigment called fucoxanthin [26].Generally, the protein content of brown seaweed is comparatively lower than the other seaweed classes.Typically, brown seaweed ranges between 5 and 15% [27], considerably lower than values reported for red (10-47%) and green seaweed (9-26%) counterparts on a dry weight basis [28].Consequently, the essential amino acid content is low in comparison.However, a wide range of soluble

Brown Seaweed
Brown seaweed, which includes the structurally complex forms of kelp, is the most common type of seaweed found in coastal areas around the world.Brown seaweed gets its name from its characteristic brown or olive-green color, which is due to the presence of a pigment called fucoxanthin [26].Generally, the protein content of brown seaweed is comparatively lower than the other seaweed classes.Typically, brown seaweed ranges between 5 and 15% [27], considerably lower than values reported for red (10-47%) and green seaweed (9-26%) counterparts on a dry weight basis [28].Consequently, the essential amino acid content is low in comparison.However, a wide range of soluble fibers, including alginates, fucans, and laminarins, are typically found in brown seaweed; however, their content varies with species [29].For instance, a substantially higher level (63.88%) of dietary fiber has been observed in Fucus spiralis compared to Spatoglossum scroederi (Phaeophyceae) (4.28%) [30].Schmid et al. [31] reported that total fatty acid levels varied substantially (0.6 to 7.8% of DW) between species, with the highest levels being found in brown seaweed (7.6% of DW), followed by green seaweed (3.9% of DW), with red seaweed (2.2% of DW) recording the lowest average concentrations.Airanthi et al. [32] studied three species of brown seaweed and found the highest n-3 PUFA content in Undaria pinnatifida (67.05 mg/g of DW), compared to Sargassum horneri (34.52 mg/g of DW), and Stephanocystis hakodatensis (formerly Cystoseira hakodatensis) (Phaeophyceae) (37.10 mg/g of DW).Moreover, saturated fatty acids (myristic acid, palmitolenic acid) are abundant in the lipid fraction of several brown seaweeds, such as Laminaria sp. and Undaria pinnatifida [29].In addition to macronutrients, brown seaweed contains a host of minerals in high concentrations, including Na, K, Ca, and Mg, as well as Fe, Zn, Mn, and Cu in lower concentrations [33].Brown seaweed also contain essential vitamins such as vitamin B, C, and E [27], whilst high levels of polyphenolic bioactive compounds, including fucol, fucophlorethol, fucodiphloroetol, and ergosterol, have also been shown to be present [34].

Red Seaweed
Red seaweed, also known as red algae, is a type of marine seaweed that belongs to the phylum Rhodophyta.They are referred to as 'red seaweed', a characteristic color, due to the presence of pigments called phycoerythrins.Red seaweed constitutes a diverse group of organisms that can be found in both freshwater and marine environments around the world [35].Red seaweed contains a higher level of protein (10-47% of DW) in comparison to green (9-26% of DW) and brown seaweed (5-15% of DW) [36].However, the protein, peptide, and amino acid concentrations of seaweed are controlled by a variety of factors, most notably by seasonal change [37].For example, the highest protein content (10% of DW) was found in red seaweed (Acanthophora muscoides) collected in the summer, which was reduced to 9% during the winter [37].The lipid content is comparatively lower in this group, ranging from 1 to 3% and 0.7 to 3% in DW in Chondrus crispus and Palmaria palmata, respectively, with apparently no significant differences between species or seasons [37,38].In terms of fatty acid composition, however, red algae have high EPA contents (1.3-10.4% DW) compared to green seaweed (0.87-2.10%).Furthermore, the soluble fiber content is usually higher in red seaweed (15-22% DW), such as Chondrus sp. and Porphyra sp.[34].Along with other minerals, the iodine level is comparatively higher in red seaweed, especially Gracilaria sp.[39].Finally, water-soluble vitamins (vitamins B and C) and carotenoids, precursors of fat-soluble vitamin A [29], as well as several biologically active substances such as monoterpenes, diterpenes, acetogenins, and sesquiterpenes, are also present in red seaweed [40].

Green Seaweed
Green seaweed refers to a group of marine seaweed that belongs to the phylum Chlorophyta.They are commonly found in coastal areas and shallow waters around the world.Green seaweed comes in a variety of shapes, sizes, and colors, ranging from small filamentous forms to large leafy or tubular structures.They typically have a green color due to the presence of chlorophyll, a pigment that enables them to photosynthesize [38].The main genus groups within Chlorophyta are Ulva, Codium, Chaetomorpha, and Cladophora [41].When comparing the protein content, the levels in green seaweed are comparatively higher compared to brown seaweed but lower than that of red seaweed.Specifically, the protein content typically ranges from around 10% to 20% of dry weight, depending on the species.Among green seaweed species, Ulva intestinalis contains up to 19.5% protein during summer [42].Fluctuations in protein content could be attributed to various external factors, such as water temperature, season, geographical location, weather, and processing conditions [42].In terms of lipid content, Ulva lactuca has a comparable lipid content (1.3% DM) to brown seaweed [43], whereas the lipid contents in Ulva australis (formerly U. pertusa) and U. intestinalis are reportedly between 2.1 and 7.4% and 7.3 and 8.7% of DW, respectively [42].Ulva is enriched with soluble and insoluble proteins, fibers, and a great source of heteropolysaccharides known as ulvan [33,44].Ulva spp.are also a great source of vitamins, particularly ascorbic acid (vitamin C) [45].
The variations in nutritional composition of the three phyla groups of seaweed are depicted in Figure 2.
weather, and processing conditions [42].In terms of lipid content, Ulva lactuca has a comparable lipid content (1.3% DM) to brown seaweed [43], whereas the lipid contents in Ulva australis (formerly U. pertusa) and U. intestinalis are reportedly between 2.1 and 7.4% and 7.3 and 8.7% of DW, respectively [42].Ulva is enriched with soluble and insoluble proteins, fibers, and a great source of heteropolysaccharides known as ulvan [33,44].Ulva spp.are also a great source of vitamins, particularly ascorbic acid (vitamin C) [45].
The variations in nutritional composition of the three phyla groups of seaweed are depicted in Figure 2.

Seaweed-Based Functional Metabolites
In addition to basic nutrients, seaweed is the source of many bioactive compounds, including polysaccharides, polyphenols, and pigments.These active compounds in seaweed have led to numerous research studies exploring their potential applications in various fields, including fish nutrition.The common bioactive compounds found in seaweeds are stated below.

Polysaccharides
Polysaccharides, the most abundant macromolecule in seaweed, are categorized into two groups based on where they are found: cell-membrane polysaccharides and storage polysaccharides.With the exception of accumulating carbohydrates found in cell plastids, the majority of seaweed polysaccharides are cell-membrane polysaccharides.Agar, carrageenan, alginate, fucoidan, laminarin, ulvan, and xylan are among the structural and storage polysaccharides found in seaweed [46,47].The chemical structures of available polysaccharides found in seaweed are presented in Figure 3. Agar and carrageenan are two of the most important polysaccharides generated from red seaweed.Some red seaweed also contains porphyrin, a sulfated polysaccharide that resembles agar and represents a highquality dietary fiber.Several studies have indicated that sulphated polysaccharides (galactans and xylans) are abundant in green seaweed, whereas fucodian, laminarin, and alginic acid are abundant in brown seaweed, and xylans, carrageenans, galactan, and porphyrin are abundant in red seaweed [9,46,48,49].The polysaccharide concentration in seaweed varies considerably, however, and has been reported to range between 4% and 76% of dry weight depending on the type of seaweed, species, location, and environmental

Seaweed-Based Functional Metabolites
In addition to basic nutrients, seaweed is the source of many bioactive compounds, including polysaccharides, polyphenols, and pigments.These active compounds in seaweed have led to numerous research studies exploring their potential applications in various fields, including fish nutrition.The common bioactive compounds found in seaweeds are stated below.

Polysaccharides
Polysaccharides, the most abundant macromolecule in seaweed, are categorized into two groups based on where they are found: cell-membrane polysaccharides and storage polysaccharides.With the exception of accumulating carbohydrates found in cell plastids, the majority of seaweed polysaccharides are cell-membrane polysaccharides.Agar, carrageenan, alginate, fucoidan, laminarin, ulvan, and xylan are among the structural and storage polysaccharides found in seaweed [46,47].The chemical structures of available polysaccharides found in seaweed are presented in Figure 3. Agar and carrageenan are two of the most important polysaccharides generated from red seaweed.Some red seaweed also contains porphyrin, a sulfated polysaccharide that resembles agar and represents a high-quality dietary fiber.Several studies have indicated that sulphated polysaccharides (galactans and xylans) are abundant in green seaweed, whereas fucodian, laminarin, and alginic acid are abundant in brown seaweed, and xylans, carrageenans, galactan, and porphyrin are abundant in red seaweed [9,46,48,49].The polysaccharide concentration in seaweed varies considerably, however, and has been reported to range between 4% and 76% of dry weight depending on the type of seaweed, species, location, and environmental factors [50].Additionally, the specific content of these polysaccharides can be influenced by the processing methods used to extract them from the seaweed for commercial and industrial purposes [50].Alginate is derived from brown seaweed, such as kelp and Laminaria species.Laminarin is generally found in Laminaria, Ascophyllum, Fucus, and Undaria sp.[51,52].
factors [50].Additionally, the specific content of these polysaccharides can be influenced by the processing methods used to extract them from the seaweed for commercial and industrial purposes [50].Alginate is derived from brown seaweed, such as kelp and Laminaria species.Laminarin is generally found in Laminaria, Ascophyllum, Fucus, and Undaria sp.[51,52].

Phenolic Compounds
Seaweed is a valuable source of polyphenolic compounds such as phlorotannins, bromophenols, flavonoids, and phenolic acids [53].Bromophenols, phenolic acids, and flavonoids make up the majority of phenolic compounds found in green and red seaweed, while phlorotannins have mostly been identified in brown seaweed [53,54].The chemical structures of available phenolics found in seaweed are displayed in Figure 4. Aqueous extracts of brown seaweed (Halopteris scoparia (formerly Stypocaulon scoparium) may contain considerable amounts of phenolic acids and flavonoids, 90 mg/100 g DW of gallic acid, followed by catechin and epicatechin (6-7 mg/100 g DW) [55].Yumiko et al. [56] investigated the flavonoid distribution in methanolic extracts of 27 Japanese seaweed species (6 green, 11 brown, and 10 red seaweeds), finding that red seaweeds had a higher amount of flavonoids than green and brown seaweeds.Hesperidin was discovered in all red seaweeds (626-119,000 µg/g), as well as some green and brown seaweeds [56].Catechol was found in all green and red seaweeds (1660-77,700 µg/g) but not in the majority of brown seaweed [56].Rutin and caffeic acids were found in all three groups but were most abundant in red seaweed (23200-4000 µg/g) [56].Quercitrin and myricetin were rarely discovered in brown and red seaweeds (202-466 µg/g), although Morin was identified in minor amounts in all seaweed samples (257-3730 µg/g) [56].Further, Onofrejova et al. [57] identified twelve phenolic acids in extracts of red seaweed (Pyropia tenera) and brown seaweed (Undaria pinnatifida), namely hydroxybenzoic acid (salicylic acid, 2,3-

Phenolic Compounds
Seaweed is a valuable source of polyphenolic compounds such as phlorotannins, bromophenols, flavonoids, and phenolic acids [53].Bromophenols, phenolic acids, and flavonoids make up the majority of phenolic compounds found in green and red seaweed, while phlorotannins have mostly been identified in brown seaweed [53,54].The chemical structures of available phenolics found in seaweed are displayed in Figure 4. Aqueous extracts of brown seaweed (Halopteris scoparia (formerly Stypocaulon scoparium) may contain considerable amounts of phenolic acids and flavonoids, 90 mg/100 g DW of gallic acid, followed by catechin and epicatechin (6-7 mg/100 g DW) [55].Yumiko et al. [56] investigated the flavonoid distribution in methanolic extracts of 27 Japanese seaweed species (6 green, 11 brown, and 10 red seaweeds), finding that red seaweeds had a higher amount of flavonoids than green and brown seaweeds.Hesperidin was discovered in all red seaweeds (626-119,000 µg/g), as well as some green and brown seaweeds [56].Catechol was found in all green and red seaweeds (1660-77,700 µg/g) but not in the majority of brown seaweed [56].Rutin and caffeic acids were found in all three groups but were most abundant in red seaweed (23200-4000 µg/g) [56].Quercitrin and myricetin were rarely discovered in brown and red seaweeds (202-466 µg/g), although Morin was identified in minor amounts in all seaweed samples (257-3730 µg/g) [56].Further, Onofrejova et al. [57] identified twelve phenolic acids in extracts of red seaweed (Pyropia tenera) and brown seaweed (Undaria pinnatifida), namely hydroxybenzoic acid (salicylic acid, 2,3-dihydroxybenzoic, p-hydroxybenzoic, protocatechuic), hydroxycinnamic acid (pcoumaric, caffeic, chlorogenic), and hydroxybenzaldehydes (3,4-dihydroxybenzaldehyde, p-hydroxybenzaldehyde).Phlorotannin is a heterogeneous group of polymeric compounds found significantly in brown seaweed that includes Laminariales (Ecklonia spp.and Eisenia spp.), Fucaceae (Ascophyllum nodosum and Fucus vesiculosus), and Sargassaceae families [58].Phlorotannin concentrations of up to 15% in dry weight (DW) have been observed in brown seaweeds, with Fucus sp.being reported as making up 12% of DW and A. nodosum being reported as making up 14% of DW [34].

Pigments
As briefly mentioned, chlorophylls, carotenoids, and phycobiliproteins are the three major groups of seaweed pigments.Seaweeds are divided into three groups depending on pigment content: seaweed that is rich in chlorophylls a or b appears green, whereas seaweed that is rich in fucoxanthin (carotenoid) appears greenish-brown, and seaweed that is rich in chlorophylls a, c, or d and phycobilins appear red [59].In general, chlorophylls are greenish, non-polar pigments that contain porphyrin or hydroporphyrin rings centrally bound to a magnesium atom, which are found in all autotrophic seaweeds.The level of chlorophyll-a ranged between 565 and 2000 mg/kg DW in brown seaweed [60].Carotenoids are lipophilic, linear polyenes in two categories: (i) carotenoids and lycopene and (ii) xanthophylls (e.g., antheraxanthin, zeaxanthin, lutein, fucoxanthin, violaxanthin).Carotenoids, which have a characteristic linear C40 chain, contain up to 11 conjugated bonds (allenic bonds) that may engage in antioxidant activities via the transfer of singlet oxygen excess energy in the long central allenic chain.Furthermore, as demonstrated in astaxanthin and fucoxanthin, the allenic bonds and other functional groups in the structure's terminal rings may react with free radicals, adding to their antioxidant capacity.The chemical structures of some common pigments found in seaweed are presented in Figure 5.A study by Balbasubramaniam et al. [61] found that carotenoids in red seaweed (Eucheuma denticulatum) consisted of lutein, zeaxanthin, β-

Pigments
As briefly mentioned, chlorophylls, carotenoids, and phycobiliproteins are the three major groups of seaweed pigments.Seaweeds are divided into three groups depending on pigment content: seaweed that is rich in chlorophylls a or b appears green, whereas seaweed that is rich in fucoxanthin (carotenoid) appears greenish-brown, and seaweed that is rich in chlorophylls a, c, or d and phycobilins appear red [59].In general, chlorophylls are greenish, non-polar pigments that contain porphyrin or hydroporphyrin rings centrally bound to a magnesium atom, which are found in all autotrophic seaweeds.The level of chlorophyll-a ranged between 565 and 2000 mg/kg DW in brown seaweed [60].Carotenoids are lipophilic, linear polyenes in two categories: (i) carotenoids and lycopene and (ii) xanthophylls (e.g., antheraxanthin, zeaxanthin, lutein, fucoxanthin, violaxanthin).Carotenoids, which have a characteristic linear C40 chain, contain up to 11 conjugated bonds (allenic bonds) that may engage in antioxidant activities via the transfer of singlet oxygen excess energy in the long central allenic chain.Furthermore, as demonstrated in astaxanthin and fucoxanthin, the allenic bonds and other functional groups in the structure's terminal rings may react with free radicals, adding to their antioxidant capacity.The chemical structures of some common pigments found in seaweed are presented in Figure 5.A study by Balbasubramaniam et al. [61] found that carotenoids in red seaweed (Eucheuma denticulatum) consisted of lutein, zeaxanthin, β-cryptoxanthin, and β-carotene, with lutein present as the major content in this class of seaweed with a concentration of 87.7 mg/100 g DW.Fucoxanthin (2740 mg/100 g DW) is the predominant carotenoid found in the brown seaweed (Sargassum polycystum), whereas β-carotene and canthaxanthin (14.6 mg/100 g and 19.5 mg/100 g DW, respectively) were the highest carotenoid contents in green seaweed (Caulerpa lentillifera).Phycobiliproteins, on the other hand, are a group of water-soluble fluorescent compounds composed of proteins covalently bound to linear tetrapyrroles known as phycobilins, and they can represent up to 40-50% of the total cellular proteins in red seaweed [62].
β-carotene and canthaxanthin (14.6 mg/100 g and 19.5 mg/100 g DW, respectively) were the highest carotenoid contents in green seaweed (Caulerpa lentillifera).Phycobiliproteins, on the other hand, are a group of water-soluble fluorescent compounds composed of proteins covalently bound to linear tetrapyrroles known as phycobilins, and they can represent up to 40-50% of the total cellular proteins in red seaweed [62].
Some of the notable bioactive compounds and their concentrations reported in various seaweeds are presented in Table 1.Some of the notable bioactive compounds and their concentrations reported in various seaweeds are presented in Table 1.

Most Commonly Utilized Seaweeds in Aquafeed
In 2019, seaweed cultivation accounted for nearly 30% of total aquaculture production.Currently, red seaweed and brown seaweed are the second and third most produced species groups in aquaculture globally, behind only "Carps, barbels and other cyprinids" [77].Currently, a variety of seaweed species are used as supplemental fish feed ingredients as sources of amino acids, fatty acids, antioxidants, vitamins, minerals, pigments, and polysaccharides.A summary of the specific types of seaweed that are most commonly used in aquafeed for fish is listed in Table 2.

The Role of Seaweed in Aquaculture Production
Seaweed is a potential source of essential nutrients that can be used as a sustainable and cost-effective supplement to traditionally used aquafeed ingredients for fish.Incorporating seaweed into the diets of farmed fish can improve their growth, health, and resistance against invading pathogens, thereby improving disease resistance.Since there is a dearth of knowledge regarding the use of seaweed functional metabolites in aquafeed for fish, the studies discussed here are mostly on the use of seaweed and seaweed-based extracts in fish nutrition.An overview of some key effects on fish production when seaweed is included in aquafeed is presented below.

Growth Performance
The effectiveness of seaweed as a feed additive varies greatly depending on the nutritional profile and the species-specific feeding nature of fish [78,79].In general, low dietary inclusion of seaweed, up to 10%, has been shown to impart significant improvements in growth, feed utilization, and the assimilation of essential nutrients [80,81].The dietary supplementation of Laminaria sp. with levels of 3 and 10% has been shown to significantly enhance the daily feed intake and weight gain in Atlantic salmon [82].Likewise, Sony et al. [83] found that dietary supplementation of fucoidan, a polysaccharide derived from brown algae (Cladosiphon okamuranus), at a level of 0.4%, significantly improved the growth performance of juvenile red sea bream (Pagrus major).In contrast, the inclusion of red seaweed (Porphyra dioica) at up to 10% of the diet did not affect the growth, whilst a 15% inclusion caused a significant growth reduction in rainbow trout (Oncorhynchus mykiss) [12].Similarly, a 6% dietary provision of Gracilaria pygmaea enhanced the growth performance of O. mykiss, while a 12% inclusion evoked negative impacts on growth [84].Moreover, Soler-vila et al. [17] reported that 10% dietary red alga (Porphyra dioica) exhibited no negative impacts on the growth of rainbow trout, while 15% inclusion showed negative results compared to the control.Overall, these observations indicate that seaweed, when incorporated at an appropriate inclusion level, can either significantly improve or maintain growth performance at similar levels to non-seaweed diets, whereas higher inclusion levels can negatively impact the growth and health status of fish.Notably, the higher growth observed with seaweed-supplemented diets is likely attributable to elevated concentrations of bioactive compounds (phytonutrients, i.e., essential vitamins and minerals) [85,86] that play vital roles in the enhanced assimilation of dietary nutrients in fish [87,88].Interestingly, it has also been speculated that seaweed contains a wide range of polysaccharides and oligosaccharides that act as prebiotics, which promote the activity of beneficial bacteria and thus enhance the digestion and absorption of essential nutrients, subsequently improving growth performance in fish [89].On the other hand, reduced growth performance at higher inclusion levels (>10%) of seaweed in aquafeed may potentially be caused by the presence of substantial concentrations of antinutritional substances emanating from the seaweed that exerts various toxicity effects and restricts the absorption of essential nutrients [89,90].For instance, protease inhibitors are found in many plant-based feeds, including seaweed.These are molecules that inhibit the activity of protease enzymes, which are responsible for breaking down proteins into smaller peptides and amino acids.When fish are fed a higher quantity of seaweed, they can bind to proteolytic enzymes and interfere with the normal digestive process by inhibiting the activity of digestive enzymes.This can lead to incomplete protein digestion, reduced nutrient absorption, and overall poor performance in fish [91].Further, instances of growth reduction may also be attributable to the polysaccharide content in seaweed, which may influence the rapid transition of feed through the digestive tract, in turn causing enhanced feed uptake while lowering the absorption of nutrients [92,93].Therefore, the removal or breakdown of these complex carbohydrates and antinutritional factors in seaweed via the incorporation of novel processing technologies may permit higher nutrient absorption efficiency and fish growth.The effects of seaweed supplementation on the growth performance of various fish species are presented in Table 3.A snapshot of some of the major effects of seaweed supplementation in aquafeed on fish performance is depicted in Figure 6.
through the digestive tract, in turn causing enhanced feed uptake while lowering the absorption of nutrients [92,93].Therefore, the removal or breakdown of these complex carbohydrates and antinutritional factors in seaweed via the incorporation of novel processing technologies may permit higher nutrient absorption efficiency and fish growth.The effects of seaweed supplementation on the growth performance of various fish species are presented in Table 3.A snapshot of some of the major effects of seaweed supplementation in aquafeed on fish performance is depicted in Figure 6.

Feed Conversion Ratio (FCR)
FCR remains a fundamental metric to assess feed efficiency in fish, where a lower value represents an improved conversion of feed to fish biomass gain.The reliance on this metric stems from the fact that feed inputs are a major cost for intensive aquaculture operations.Several feed additives, including seaweed, have been incorporated into aquafeed to improve the FCR.Several studies reported that dietary seaweed inclusion resulted in a lower FCR in Nile tilapia [21,94], Salmo salar [82], Pagrus major [83], Acanthopagrus schlegelii [95], and Labeo rohita [96].Improvements to FCR could be partly due to the presence of various bioactive compounds (carotenoids, polysaccharides, amino acids, and fatty acids) that significantly improve the palatability and, consequently, intake of feed, hence improving feed utilization [97].Bioactive substances have been shown to stimulate the secretion of several enzymes (amylase, lipase, and protease) that are known to enhance the digestion of essential nutrients as well as their assimilation into fish tissues [98].Likewise, improved FCR could result from the activities of the polysaccharides of seaweed that slow the passage of feed through the digestive tract, which ensures greater nutrient assimilation and bioavailability [84,99].In addition, seaweed as a source of prebiotics may enhance the growth of beneficial bacteria in the gut, significantly improving digestibility and feed efficiency [100].However, contrasting findings to those articulated above have been reported, with several studies indicating that dietary seaweed did not significantly affect feed utilization across a range of fish species, including seabass (Dicentrarchus labrax), Senegalese sole (Solea senegalensis), and gilthead seabream (Sparus aurata) [81,101,102].Notably, high levels of seaweed in aquafeed may reduce the palatability of fish [103].Moreover, these variations may be exhibited by the feed composition, physiology of fish species, size of the species, environmental quality, as well as the dietary inclusion level of the seaweed.

Feed Palatability
The palatability of aquafeed is one of the most crucial factors influencing the consumption of feeds by farmed species [104].A reduction in palatability may lead to an increase in feed wastage, resulting in reduced fish production and negatively impacting the profitability of aquaculture operations.On the contrary, highly palatable feeds increase feed consumption and effectiveness, generally resulting in better fish growth, assuming the nutritional requirements of the species are being met (Table 3).However, the palatability of an aquafeed is largely influenced by the nutrient composition of its ingredients as well as feed processing techniques, nutrient digestibility, water stability, and species-specific nutritional requirements and physiology of fish [105].The application of several plant-origin protein sources, including those emanating from algal species, to enhance the palatability of fish feed has attracted considerable research attention in recent times.Kamunde et al. [82] reported that Atlantic salmon consumed more feed when brown seaweed (Laminaria sp.) was included in the diet in comparison to a seaweed-free control feed.Similarly, greater consumption of an Ulva sp.-based diet was reported in seabream (S. aurata) [106] and sea urchin (Tripneustes gratilla) [107].This higher feed response may be attributable to several bioactive compounds such as dimethyl-beta-propionthein, dimethyl sulfonyl propionate, amino acids, and peptides that enhance the attraction of a feed to the farmed fish species and, in turn, increase feed consumption [108,109].In addition, the inclusion of seaweed can improve the overall physical structure of an aquafeed, including integrity, texture, and water stability, all of which are factors that may contribute to increased feed intake [110].Furthermore, the volatile organic compounds emitted by seaweed [111] can contribute to the aroma and flavor of the feed, making it more attractive and palatable to fish.This could potentially lead to increased feed intake and improved growth rates.However, the application of seaweed in aquafeed should be carefully considered as high inclusion levels have been reported to reduce feed palatability and feed consumption, in turn negatively impacting the growth and health status of fish [90].

Feed Digestibility
The efficiency of an aquafeed is highly dependent on the digestibility of its constituent feed ingredients (Table 3).The incorporation of ingredients with a high digestible value will minimize feed wastage and maximize feed utilization, thus improving growth performance.It has been reported that apparent nutrient digestibility coefficients (ADC) of protein, lipid, and energy were not changed when up to 20% of Ulva sp. was included in diets for Nile tilapia [112].Pereira et al. [113] revealed that the digestibility of Ulva meal in diets for Nile tilapia was higher in comparison to diets containing Gracilaria or Porphyra.Contrarily, Soler-vila et al. [12] found that up to 15% inclusion of Porphyra dioica did not result in significant alterations in comparison to a control diet in rainbow trout.However, Azaza et al. [92] reported that a 10% replacement of soybean meal with Ulva rigida decreased the ADC of protein in Nile tilapia from 87% to 82%.Notably, the effect of seaweed inclusion on nutrient ADC appears to be dependent on the type of seaweed itself, the nature of fish species, feed composition, and the degree of inclusion of the examined seaweed and, thus, the protein source being substituted.Different seaweed species exhibit varying effects on nutrient digestibility in different fish species, which can largely be explained by feeding habits and gut morphology, which determines the capacity for digestion and absorption of the nutrients contained in seaweed [114].Most herbivorous and omnivorous fish species exhibit a higher level of amylase activity for the enhanced breakdown of the carbohydrates provided by dietary seaweed inclusion [80,115].Importantly, carnivorous fish species have a reduced ability to break down complex seaweed polysaccharides due to a lack or limited amount of these enzymes [116].As such, coupling digestive enzyme activity with the morphology of the gastrointestinal tract, the capacity for dietary seaweed incorporation into aquafeeds will likely be dictated by trophic level, where it stands to reason that herbivorous and omnivorous species will have a much higher tolerance to dietary seaweed than their carnivorous counterparts.Note: SW-seaweed; FM-fish meal; WG-weight gain; FCR-feed conversion ratio; PER-protein efficiency ratio; FE-feed efficiency; ADC-apparent daily co-efficient; FI-feed intake; PM-poultry meal; GOT-glutamicacid-oxyl acetic-acid-transaminase; SOD-superoxide dismutase; GP-glutathione peroxidase; PO-phenoloxidase; LYZ-lysozyme activity; CAT-catalase activity; ACH50-alternative complement activity; MPO-myeloperoxidase; NBT-nitroblue tetrazolium; RBC-red blood cell; WBC-white blood cells; Hb-haemoglobin; AST-aspartate transaminase; ALT-alanine transaminase; ROS-reactive oxygen species; NO-nitric oxide; --not identified.

Immunity and Disease Resistance
Disease remains a great threat to the intensive aquaculture sector, hindering industry growth and potentially leading to huge economic losses [158].The main purpose of intensive aquaculture systems is to ensure maximum production within a limited culture period.In some circumstances, on-farm efficiency may be improved by operating at high stocking densities, potentially causing a deterioration of water quality to the detriment of fish health through the suppression of the immune system and the disruption of antioxidant defense mechanisms.To address these challenges, different types of drugs are commonly used for the treatment of disease [159].However, their indiscriminate use has led to growing concerns for the surrounding environment as well as public health via the direct consumption of treated farmed fish or through the consumption of wild fishes located in the areas surrounding the treated aquaculture farm [160,161].Furthermore, the rapid application of antibiotics may give rise to antibiotic-resistant bacteria [162] that significantly reduce the efficacy of antibiotics in controlling diseases.Therefore, it is of the utmost importance to seek possible environmentally friendly prophylactic measures.The application of seaweed-based feed ingredients as immunostimulants to strengthen the immune status of fish is viewed as a suitable alternative.An overview of the role of seaweeds as immunostimulators in fish is presented in Table 3. Mendonca et al. [163] revealed that 5% dietary Gracilaria domingensis (Rhodophyta) improved the immune response of juvenile mullet (Mugil liza) by modulating the activity of glycoproteins CD3 and CD4.Similarly, 6% dietary S. hornei promoted the antioxidant profile and immune capacity of black sea bream (Acanthopagrus schelegelii) [95].Likewise, the green seaweed (Ulva lactuca (formerly Ulva fasciata) (Chlorophyta)) greatly improved the innate immune response of Nile tilapia through the modulation of lysozyme and phagocytic activity, as well as total WBC count and overall antioxidant status [164].Similarly, the addition of red microalga (Porphyridium sp.) in the diet of pompano (Trachinotus ovatus) significantly upregulated the levels of mRNA c-type lysozyme and complement C4 while downregulating the mRNA heat shock protein (HSP70), thus playing an important role in the improvement of non-specific immune responses [165].On the contrary, besides the positive role of seaweeds and their derivatives, the review of Thepot et al. [6] revealed no significant impacts in stimulating growth and immune responses in fish.This may be attributed to the very short (14-day) feeding trial that was likely insufficient to exhibit a significant immune response, given most immunostimulating compounds are reported after longer (49-day) feeding trials [166].
Alternatively, Wang et al. [167] observed that dietary Sargassum horneri (Phaeophyceae) did not significantly alter the lysozyme activity of juvenile turbot (Scophthalmus maximus) in comparison to the control.This variation may be caused by genetically driven speciesspecific responses of fish to the associated seaweed species.In addition, diseases in the aquaculture sector cost the industry in excess of USD 6 billion each year [168].Fish are often subjected to various pathogenic organisms, which can lead to several diseases that negatively impact their health status and growth performance.In this situation, the utilization of seaweed and its bioactive substances as suitable alternative strategies can enhance both the cellular and humoral immune response towards disease resistance.Zeraatpisheh et al. [141] reported that the supplementation of Sargassum angustifolium (Phaeophyceae) in the diet of rainbow trout positively modulated fish immunity via increased hemoglobin (Hb), hematocrit (Hct), red blood cells (RBC), white blood cells (WBC), and phagocytes.Likewise, elevated lysozyme activity (LYZ), the expression of immune-related genes (e.g., il-1β, tnf-α), and the modulation of resistance to pathogenic bacterial infection from Yersinia rukeri (Pseudomonas fluorescens) were also reported.Several recent studies have also demonstrated that dietary provision of Sargassum ilicifolium (Phaeophyceae), Gracilaria sp.(Rhodophyta), Ulva ohnoi, and Sarcodia suiae (Rhodophyta) improved the nonspecific immunity and disease resistance of great sturgeon (Huso huso) [169], Dicentrarchus labrax [142], Solea senegalensis [170], and O. niloticus [171], respectively.In addition, Wang et al. [167] revealed that dietary Sargassum horneri (Phaeophyceae) significantly enhanced the non-specific immunity of juvenile turbot (S. maximus) and its disease resistance against Edwardsiella tarda.

Antioxidant Response
Seaweed and its extracts exhibit excellent antioxidant and immunomodulatory properties [172,173].Inoculation of seaweed extracts (sodium alginate and carrageenan from Macrocystis pyrifera and Chondrus crispus) in grouper (Epinephelus coicoides) resulted in a significant enhancement of respiratory burst; superoxide dismutase and phagocytic activities that are the key indicators of antioxidant status [155].Peixoto et al. [18] reported that a 2.5% inclusion of dietary Gracilaria spp.significantly promoted glutathione peroxidase (GPx) activity in European seabass, which may be attributed to the elevated levels of selenium found in Gracilaria spp. that contribute to increased GPx production [174,175].In addition to enhanced lipid peroxidation, increases in glutathione reductase and glutathione s-transferase have been reported as a result of dietary Gracilaria inclusion [81], clearly indicating the influence of Gracilaria sp.inclusion with respect to the modulation of fish antioxidant profile and the stress status of fish.Moreover, in Atlantic salmon, the supplementation of Laminaria sp.not only significantly increased the total plasma antioxidant status but also activated several mitochondrial antioxidant enzymes, including catalase, superoxide dismutase (SOD), and total glutathione level [82].Similar results have also been reported in rainbow trout fed diets supplemented with Gracilaria pygmaea [84].In addition, the dietary provision of either whole brown seaweed (Ascophyllum nodosum) or its extract significantly modulated the serum antioxidant profile, lowered lipid peroxidation, and enhanced the activity of SOD in ruminants [176,177].All of these findings clearly highlight the potential role of seaweeds in modulating the antioxidant status of fishes either directly via an elevation of antioxidant substances or via an improvement to the functioning of antioxidant defense mechanisms.

Intestinal Morphology
The intestines of fish are important organs that play a vital role in fish's immune statuses [178].The morphological structure of the gastrointestinal tract (GIT) acts as an important indicator of the nutritional status and physiological state of fish [179].Several studies have reported that dietary seaweeds, such as Sargassum dentifolium (S. ilicifolium) (Phaeophyceae), do not alter the normal intestinal tissue structure (e.g., enterocyte length and width and thickness of villi) [143,180], indicating its suitability as a feed ingredient.Dietary seaweed supplementation has also been shown to improve the intestinal epithelial mucosa, indicating an enhanced immune capacity of fish [91].The immune activities of fish intestines are greatly dependent on the condition of the associated intestinal barriers that primarily consist of epithelial cells [181].These epithelial cells assist in the production of IgA through the activation of T cells and B cells that play a defensive role against various antigens [181].Dietary Laminaria digitata (Phaeophyceae) and Gracilaria gracilis (Rhodophyta) significantly boosted the intestinal acid goblet cells of mullet (Liza ramada) [182] and European seabass (D. labrax) [183,184], which perform key roles in intestinal immune activity.Goblet cells act as a protector of intestinal barriers through the production and secretion of mucus and antimicrobial proteins (chemokines and cytokines), enhancing the local immune response of the intestine [185].Yu et al. [103] demonstrated that Gracilariopsis lemaneiformis provision in the diet of Litopenaeus vannamei increased the villi length of the intestine, which significantly improved the absorption capacity of several nutrients.Similarly, the provision of 1 to 3% Undaria pinnatifida in diets for shrimp (Penaeus monodon) significantly enhanced the length of intestinal fold when compared to the control [186].On the contrary, 10% Gracilaria sp.supplementation caused lower villi length and diameter in Nile tilapia [187] and rainbow trout [17], which negatively affected the nutrient utilization and hence, growth of the associated species.These variabilities reported here could result from the presence of several antinutritional factors (phytic acid, saponin, and tannins) in seaweeds that alter the structure of the intestine and negatively affect the digestion process [92,188].The effects of seaweed supplementation on the histo-morphological structures of various fish species are presented in Table 4. Note: SW-seaweed; GI-gastrointestinal tract; --not identified.

Gut Microbiota Composition
The study of fish gut microbiota has attracted significant research attention in recent years [208].Gut microbiota plays an important role in fish growth, nutrition, immunity, and resistance against pathogenic microorganisms [4,209].The fish gut acts as an assemblage of sever al microbial communities, and their activities greatly influence different aspects of fish physiology [210].An overview of the role of seaweed on gut microbiota composition in fish is presented in Table 4.The microbial communities in fish guts vary greatly depending on several factors such as the physiological state of the gut, trophic level and environment, and dietary ingredients [211,212].Seaweed has been shown to be a promising feed additive that can modulate the gut microbial composition of fish [196].The dietary inclusion of Gracilaria gracilis enhanced the abundance of the microbes Sulfitobacter and Methylobacterium in the gut of European seabass [197].These microbes are capable of producing short-and medium-chain fatty acids and can lower the pH of the intestine, thus playing a crucial role in suppressing pathogenic bacteria and potentially representing a promising method of enhancing disease resistance in fish [213,214].Similarly, dietary supplementation of fucoidan, a polysaccharide derived from brown seaweed (Undaria pinnatifida), is reported to elevate the intestinal digestive enzyme activities and, thereby, modulate the intestinal microbial communities in gibel carp (Carassius auratus gibelio) when added at a level of 30 g/kg WW [190].Furthermore, the provision of S. dentifolium (3 g/kg of diet DW) extract significantly lowered the abundance of pathogenic microorganisms in the gut of Pacific white shrimp (Litopenaeus vannamei) [100].On the contrary, a high inclusion (8%) of G. gracilis resulted in a significant reduction of gut microbial diversity.However, these negative impacts were mitigated at a lower inclusion level (4%) [197].Tapia-paniagua et al. [198] reported that a relatively low (<3%) dietary administration of Ulva ohnoi significantly enhanced the diversity of whole gut microbes in Senegalese sole (Solea senegalensis), while 5% U. ohnoi did not exhibit a significant influence on gut microbial diversity [196].These variable results could be attributed to the specific adaptative response of different microbial communities across a range of feeding schedules, such as time and duration of feeding and feeding frequencies.Nevertheless, dietary U. ohnoi reduced the abundance of the genus Escherichia [198] in S. senegalensis, which could be attributed to the antibacterial properties of Ulva spp.against Escherichia coli [215,216].Further, Xinxu et al. [206] reported that dietary Ulva australis (formerly Ulva pertusa) (Chlorophyta) enhanced the abundance of several bacterial species of the Firmicutes group, including Ruminococcus, Clostridium, and Lachnospiraceae in white-spotted rabbitfish (S. canaliculatus) that actively participate in the degradation of non-starch polysaccharides in the host gut [217,218].These results indicate that seaweed inclusion in aquafeed can be beneficial up to a certain extent, while the excessive inclusion of seaweed in aquafeed can lead to negative effects, including reductions in the growth of beneficial gut bacteria, leading to poor digestion and nutrient absorption, which may weaken fish immune systems and subsequently increase susceptibility to disease.The potential impacts of dietary seaweed inclusion or their extracts on the intestinal health of fish are depicted in Figure 7.

Potential Limitations and Future Perspectives
While seaweed offers many advantages as a potential ingredient for aquafeed, there are some potential disadvantages that need to be considered.A summary through SWOT (strength-weakness-opportunity-threat) analysis of seaweed utilization as an aquafeed ingredient and source of bioactive metabolites is presented in Table 5.These will be addressed below while also discussing potential mitigation strategies and future perspectives.
Significant industry expansion required globally: Commercial seaweed farming takes place in over 35 countries worldwide, but the bulk (98.9%) is concentrated in China (60%) and Southeast Asia, notably Indonesia (21%), the Philippines (9%), and Malaysia (1%) [219].Small subsistence farms (1 ha) abound throughout Southeast Asia, and their expansion is largely regulated by both access to usable habitats and proximity to markets.Therefore, seaweed production must continue to expand into other regions as the demand for seaweed and seaweed derivatives grows.Clearly, the expansion of the seaweed aquaculture industry into other parts of the world will be subject to both logistical and regulatory constraints, and this will ultimately determine the viability of continued industry expansion.As such, regionally specific viability assessments for seaweed aquaculture will be continually required to meet the growing needs within both the human and animal feed sectors.The potential effects of seaweed and seaweed-based functional metabolites in improving the gut health of fish.An optimal dietary inclusion of seaweed in aquafeed stimulates gut microbiota and improves immune response, whereas excessive inclusion is reported to suppress growth, mask immune response, and distort gut microbiota.Created with BioRender.comaccessed on 25 September 2023.

Potential Limitations and Future Perspectives
While seaweed offers many advantages as a potential ingredient for aquafeed, there are some potential disadvantages that need to be considered.A summary through SWOT (strength-weakness-opportunity-threat) analysis of seaweed utilization as an aquafeed ingredient and source of bioactive metabolites is presented in Table 5.These will be addressed below while also discussing potential mitigation strategies and future perspectives.
Significant industry expansion required globally: Commercial seaweed farming takes place in over 35 countries worldwide, but the bulk (98.9%) is concentrated in China (60%) and Southeast Asia, notably Indonesia (21%), the Philippines (9%), and Malaysia (1%) [219].Small subsistence farms (1 ha) abound throughout Southeast Asia, and their expansion is largely regulated by both access to usable habitats and proximity to markets.Therefore, seaweed production must continue to expand into other regions as the demand for seaweed and seaweed derivatives grows.Clearly, the expansion of the seaweed aquaculture industry into other parts of the world will be subject to both logistical and regulatory constraints, and this will ultimately determine the viability of continued industry expansion.As such, regionally specific viability assessments for seaweed aquaculture will be continually required to meet the growing needs within both the human and animal feed sectors.Nutrient variability: Seaweeds are known to have variable nutrient compositions depending on factors such as species, growing conditions, and harvesting season [220].This variability can make it challenging to formulate consistent and balanced diets for farmed aquatic organisms.To combat this, additional compositional analysis and feed formulation adjustments are required to ensure the nutritional requirements of the target species are met consistently.
Limited availability: Seaweeds have specific growth seasons, and their availability can be influenced by factors such as temperature, light, and nutrient availability [221].Seasonal fluctuations in seaweed production can affect the stability and continuity of the aquafeed supply chain, potentially leading to feed shortages or increased costs during certain times of the year [222].Therefore, understanding seasonal patterns, diversification of seaweed farming techniques (i.e., floating or suspended cultivation systems), and postharvest preservation could be maintained to create a more stable and sustainable seaweed production system.
Lower inclusion level: The benefits of dietary seaweed inclusion are generally best realized when included in aquafeed at levels of around 5%.Beyond this level, a host of negative impacts on growth and feed utilization in fish have been reported, in part, due to palatability issues.Therefore, more research is needed to determine the most appropriate seaweed species and their optimal inclusion levels in aquafeed for fish and also whether their inclusion is most appropriate when utilized as a minor ingredient or functional additive.Furthermore, fermentation or enzymatic processing should be explored to improve the functionality of seaweed inclusion in aquafeed for farmed fish.
Processing challenges: The processing of seaweed into suitable raw materials for inclusion into aquafeed can present certain challenges.Namely, seaweeds have a high moisture content, which needs to be reduced to enhance shelf life and prevent spoilage.Processing methods, such as drying or grinding, inevitably require energy and infrastructure, which can add to the overall cost of production.
Palatability and acceptance: Seaweeds have distinct flavors, textures, and high ash contents that may not be universally accepted by all species of farmed fish [223].Some fish species may show lower feed intake or reduced growth rates when seaweed-based diets are used.For example, excessive ash can lead to reduced digestibility of feed since most aquaculture species have their specific nutritional requirements.The application of fermentation or enzymatic processing may increase seaweed palatability.However, encouraging feed acceptance and addressing palatability issues might require additional research and formulation adjustments.
Heavy metals contamination: Hazardous levels of heavy metals (such as cadmium, lead, mercury, and arsenic) and iodine in some seaweed species may pose a barrier to their use in aquafeed [224].Furthermore, the presence of antinutritional components, radioactive isotopes, ammonium, dioxins, and pesticides is an important consideration.Previous research has also shown that wild seaweed may include traces of plastic particles, raising concerns about the toxicity of seaweed in aquafeed.More feeding and clinical experiments are needed to better understand the bioavailability of pollutants and other limiting variables that may prevent its utilization in aquafeed.

Conclusions
The nutritional and functional properties of seaweed attest to their potential to be incorporated into aquafeed to safeguard fish growth and health as the global demand for fish and seafood products rapidly increases.An increasing body of research demonstrates that seaweed and seaweed-derived functional metabolite supplementation has a major positive effect on growth, physiological stress resilience, and immune response in fish.However, additional research into the challenges of utilizing seaweed is warranted, specifically on the effect of seaweed supplementation on nutrient digestibility, the potential of long-term effects on fish health, and the possible interactive effects between dietary seaweed supplementation with high dietary inclusion levels of terrestrial plant proteins and carbohydrates.Furthermore, the potential effects of seaweed and its immunomodulatory compounds should be further explored in fish to elucidate their underlying physiological mechanisms.Such research is deemed necessary to fully understand the existing commercial potential of seaweed in aquaculture and in order to advance this emerging field of research.Finally, the standardization of study conditions and subsequent reporting of key metrics relating to fish health and performance will aid in the elucidation of optimal inclusion levels for seaweed and seaweed-derived bioactives in aquafeed for a multitude of species.Ultimately, this will support the future widespread use of seaweed and seaweed-derived bioactives in aquaculture production.

Figure 1 .
Figure 1.Schematic representation of (a) red (Rhodophyta), (b) green (Chlorophyta), and (c) brown (Phaeophyceae) seaweed utilized as feed additives and sources of bioactive compounds in aquafeed for fish.Seaweed drawings are courtesy of the Integration and Application Network, University of Maryland Center for Environmental Science (ian.umces.edu/symbols/accessed on 25 September 2023).

Figure 1 .
Figure 1.Schematic representation of (a) red (Rhodophyta), (b) green (Chlorophyta), and (c) brown (Phaeophyceae) seaweed utilized as feed additives and sources of bioactive compounds in aquafeed for fish.Seaweed drawings are courtesy of the Integration and Application Network, University of Maryland Center for Environmental Science (ian.umces.edu/symbols/accessed on 25 September 2023).

Figure 2 .
Figure 2. The nutritional composition (% of dry weight, DW) of various seaweed (red, green, brown) used in aquafeed formulations for fish.Approximately 30 research articles spanning 17 seaweed species were compiled for each value, demonstrating the average nutritional constituents of various seaweed species for the study.

Figure 3 .
Figure 3.The chemical structures of various types of polysaccharides found in seaweed.

Figure 3 .
Figure 3.The chemical structures of various types of polysaccharides found in seaweed.

Figure 4 .
Figure 4.The chemical structures of various types of phenols found in seaweed.

Figure 4 .
Figure 4.The chemical structures of various types of phenols found in seaweed.

Figure 5 .
Figure 5.The chemical structures of various types of pigments found in seaweed.

Figure 5 .
Figure 5.The chemical structures of various types of pigments found in seaweed.

Figure 6 .
Figure 6.Schematic diagram demonstrating the composition of seaweed and the potential impacts of its addition on growth and health performance of fish.

Figure 7 .
Figure 7.The potential effects of seaweed and seaweed-based functional metabolites in improving the gut health of fish.An optimal dietary inclusion of seaweed in aquafeed stimulates gut microbiota and improves immune response, whereas excessive inclusion is reported to suppress growth, mask immune response, and distort gut microbiota.Created with BioRender.comaccessed on 25 September 2023.

Figure 7 .
Figure7.The potential effects of seaweed and seaweed-based functional metabolites in improving the gut health of fish.An optimal dietary inclusion of seaweed in aquafeed stimulates gut microbiota and improves immune response, whereas excessive inclusion is reported to suppress growth, mask immune response, and distort gut microbiota.Created with BioRender.comaccessed on 25 September 2023.

Table 2 .
Major seaweeds used in aquafeed as the sources of nutrients and bioactive compounds for fish.

Table 3 .
Effects of seaweed and seaweed-based functional metabolites on growth, feed utilization, immunity, and disease resistance in farmed fish (studied parameters were compared to control-0% FM diet).

Table 4 .
The effects of seaweed and seaweed-based functional metabolites on gut histo-morphometry and gut microbiota composition in farmed fish (studied parameters were compared to control-0% FM diet).

Table 5 .
SWOT analysis of seaweed utilization as aquafeed ingredient and source of feed additives (seaweed-based bioactive metabolites) in aquaculture production.