The Beneficial Effects of Natural Extracts and Bioactive Compounds on the Gut-Liver Axis: A Promising Intervention for Alcoholic Liver Disease

Alcoholic liver disease (ALD) is a major cause of morbidity and mortality worldwide. It can cause fatty liver (steatosis), steatohepatitis, fibrosis, cirrhosis, and liver cancer. Alcohol consumption can also disturb the composition of gut microbiota, increasing the composition of harmful microbes and decreasing beneficial ones. Restoring eubiosis or preventing dysbiosis after alcohol consumption is an important strategy in treating ALD. Plant natural products and polyphenolic compounds exert beneficial effects on several metabolic disorders associated with ALD. Natural products and related phytochemicals act through multiple pathways, such as modulating gut microbiota, improving redox stress, and anti-inflammation. In the present review article, we gather information on natural extract and bioactive compounds on the gut-liver axis for the possible treatment of ALD. Supplementation with natural extracts and bioactive compounds promoted the intestinal tight junction, protected against the alcohol-induced gut leakiness and inflammation, and reduced endotoxemia in alcohol-exposed animals. Taken together, natural extracts and bioactive compounds have strong potential against ALD; however, further clinical studies are still needed.


ALD: Epidemiology, Progression, Pathogenesis, and Treatment
Alcohol abuse is the fifth leading cause of disease and death worldwide. Around 2.4 billion people drink alcohol globally, including 1.5 billion (1.4-1.6) men and 0.9 billion (0.8-1.0) women [1]. However, drinking condition differs from country to country. For instance, the total per capita intake of alcohol in France is 12-13 L/adult, followed by the United Kingdom, Eastern Europe, United States of America, Italy, and North Africa/Middle East of 11-12 L/adult, 11-13 L/adult, 10 L/adult, 7 L/adult, and only 0-2 L/adult, respectively [2].
Alcohol is a major hazard of alcoholic liver disease (ALD), which begins with fatty liver disease (steatosis) and later progresses to alcoholic steatohepatitis (ASH) and fibrosis [3,4]. Distinguishing between acute and chronic alcohol intake and its impact on ALD prognosis is critical [5]. Although alcohol intake is the main cause of ALD, environmental variables and genetics may contribute to its progression [6]. Steatosis is one of several initial manifestations of ALD, essentially defined by an oversized liver. ALD can lead to serious consequences, such as cirrhosis and hepatocellular carcinoma, and there are currently no FDA (Food and Drug Administration)-approved treatments [4].

Figure 1.
Alcohol abuse significantly affects many microbes in the gut. ↑, the abundance was upregulated; ↓, the abundance was down-regulated.

Dysregulation of Bile Acid Metabolism
One of the significant communicators between the intestine and liver is bile acids. The hepatic biliary system secretes conjugated bile acids, which are converted as needed by intestinal bacteria in the duodenum [43]. Then the modified bile acids enter the enterohepatic circulation and reach the liver again. As previously mentioned, liver cirrhosis lowers normal bile flow in patients [44]. Bile acids stimulate the farnesoid X receptor in intestinal epithelial cells, leading to the induction of antimicrobial molecules [45]. On the contrary, the overgrowth of intestinal bacteria results from decreased bile flow. The experimental model of alcohol feeding in rodents showed a correlation between bile acid metabolism and the intestinal microbiome. Due to ethanol intake, taurine-conjugated bile acids were reduced in rats' intestines and livers [46]. Nevertheless, the level of glycineconjugated and unconjugated bile acids increased [47]. The partial reason behind this could be the overgrowth of gastrointestinal bacteria as cirrhosis patients exhibit escalated bile acids deconjugation [48]. Chronic abuse of alcohol in patients raises the total amount of bile acids, secondary bile acids, lithocholic acid, deoxycholic acid, and secondary-to- Figure 1. Alcohol abuse significantly affects many microbes in the gut. ↑, the abundance was upregulated; ↓, the abundance was down-regulated.
Collectively, chronic alcohol administration or intake results in dysbiosis, which is related to a decrease in beneficial bacteria. Restoring eubiosis or preventing dysbiosis after alcohol intake is an important strategy against ALD.

Dysregulation of Bile Acid Metabolism
One of the significant communicators between the intestine and liver is bile acids. The hepatic biliary system secretes conjugated bile acids, which are converted as needed by intestinal bacteria in the duodenum [43]. Then the modified bile acids enter the enterohepatic circulation and reach the liver again. As previously mentioned, liver cirrhosis lowers normal bile flow in patients [44]. Bile acids stimulate the farnesoid X receptor in intestinal epithelial cells, leading to the induction of antimicrobial molecules [45]. On the contrary, the overgrowth of intestinal bacteria results from decreased bile flow. The experimental model of alcohol feeding in rodents showed a correlation between bile acid metabolism and the intestinal microbiome. Due to ethanol intake, taurine-conjugated bile acids were reduced in rats' intestines and livers [46]. Nevertheless, the level of glycine-conjugated and unconjugated bile acids increased [47]. The partial reason behind this could be the overgrowth of gastrointestinal bacteria as cirrhosis patients exhibit escalated bile acids deconjugation [48]. Chronic abuse of alcohol in patients raises the total amount of bile acids, secondary bile acids, lithocholic acid, deoxycholic acid, and secondary-to-primary bile acid ratio in the stool [49]. If any patient develops advanced cirrhosis, they show an increment in serum level of conjugated bile acids and a reduction in the amount of total bile acids [49,50]. The secretion dimension of bile acid in the intestine of cirrhotic patients may be the underlying cause of both phenomena [44]. For a better understanding of pathogenesis related to chronic alcohol abuse and to develop potential therapeutic agents, extensive research is needed to further explore the interactions between bile acids and gut microbiota. This bidirectional crosstalk can better define the communications between the liver and intestine.

Microbial Products Contribute to Liver Inflammation and Disease
The liver readily absorbs toxins from the portal vein circulation as the unadulterated intestinal products reach the liver first. The promoters of hepatocellular injury are microbial toxins; including microbial pathogen-associated molecular patterns (PAMPs), fungal exotoxins (such as candidalysin), bacterial exotoxins (such as cytolysin secreted by Enterococcus), bacterial endotoxins (such as lipopolysaccharide [LPS] from gram-negative bacteria), hepatic toll-like receptors activated by endotoxins, and PAMPs that directly interact with pattern-recognition receptors present on hepatic stellate and Kupffer cells. The microbial products can advance cytokine stimulation, fibrotic changes, and oxidative stress (OS) of the inflammatory cascade [51].
Specific exotoxins exert pathogenicity in ALD patients. Compared to heavy drinking control, patients with AH show an increased abundance of Enterococcus faecalis, a cytolysinproducing bacteria. The quantity of cytolysin is associated with both the mortality and extremity of disease, the same as the fungal exotoxin candidalysin, which is also found in higher concentrations in AH patients [52]. Ethanol-comprising diet worsened liver injury in candidalysin-producing Candida colonized mice [53].
Studies have shown that dysbiosis may be related to the amount of endotoxins that circulate freely. Dysbiosis in ALD patients, along with AH and alcohol-related cirrhosis patients, revealed a correlated upsurge in flowing LPS [54][55][56]. Alcohol-related cirrhosis seemed to show a greater degree of endotoxemia than non-alcohol-induced cirrhosis, despite the end-stage liver disease scores being irrelevant [54]. Intestinal permeability is probably a vital implementer of endotoxemia. Markedly, half of the patients with ALD (about half of alcohol use disorders patients) showed increased permeability in the intestinal barrier, revealing a close association with microbiome changes. Therefore, increased intestinal permeability caused by microbial dysregulation is an important prerequisite for the progression of ALD [57,58].

Short-Chain Fatty Acids (SCFAs)
There are numerous processes involved in the regulation of intestinal permeability. Consumption of alcohol can affect many of those processes. Chronic alcohol intake deteriorates dysbiosis and subsequently disturbs the integrity of the tight junctions of the enterocyte, as SCFA-producing commensals are involved in maintaining barrier integrity [51]. Additionally, hepatic inflammation and adiposity can be mitigated by SC-FAs [59]. ALD patients exhibited a consistent decrease in the microbiomes of Lachnospiraceae and Ruminococcaceae families [42,50,54,[60][61][62]. Conversely, Veillonella is also known to produce SCFAs and is often expanded in patients with ALD [50,60,62,63]. There was a significant reduction in SCFAs in the feces of AH patients compared to heavy drinkers, despite changes in specific microbial patterns [60]. In short, intestinal permeability increases with the fading production of SCFAs, ultimately leading to hepatic inflammation.

Endotoxin
Endotoxin is one of the main components of the outer membrane of the gram-negative bacteria cell wall. Compared to non-alcoholic subjects, plasma of alcohol abused patients contained a 5-fold higher concentration of endotoxin [64]. Intake of alcohol disturbs the intestinal barrier functions and magnifies intestinal permeability. In the rat model, alcohol administration enabled systemic translocation and absorption of endotoxin [65]. The intensity of ethanol-induced liver injury in rats was significantly interrelated with endotoxin levels in plasma [66]. Endotoxin can cross the intestinal barrier and activate the Kupffer cells that generate TNF-α and superoxide in the liver, resulting in severe hepatic damage [67].

Bioactive Compounds against Alcohol-Induced Gut Microbiota Dysbiosis
Phenolic compounds are a large group of chemicals, such as phenolic acids, flavonoids, stilbenes, lignans, and other chemicals, commonly present in various edible plants. Phenolic compounds possess countless health benefits, including the hepatoprotective effect [26,27]. Earlier, Yuan et al. reported that epigallocatechin-3-gallate (EGCG) protected ALD via inhibiting alcohol-induced gut leakiness and inflammatory factors expressions, and reducing endotoxemia in rats [26]. Later, another study documented that EGCG acted as a prebiotic for L. plantarum, developing microbead synbox, and was promising as a therapeutic option for the ALD [28]. Similarly, other polyphenolic compounds, puerarin and kaempferol, alleviated ALD in mice via regulating intestinal tight junctions and inhibiting endotoxin leakage [29,30].
Earlier, tributyrin supplementation was found to protect mice from alcohol via expression and co-localization of tight junction (TJ) proteins (ZO-1, occludin), as well as butyrate receptor (GPR109A) and transporter (SLC5A8) in the ileum and proximal colon [68]. Aplysin, a brominated sesquiterpene compound purified from red alga Laurencia tristicha was studied against ALD. The daily treatment of aplysin (150 mg/kg bw) for 12 weeks markedly modulated the composition of Escherichia coli, Bacteroides fragilis, Lactobacillus, Bifidobacterium, and other key biomarkers, thus protecting ALD [69].
Berberine is a natural compound present in many plant extracts and possesses multiple health effects. Recently, Li et al. conducted a study to explore the protective effect of berberine against alcohol-mediated gut microbiota dysbiosis. Berberine (10, 50, and 100 mg/kg bw) was orally administrated to the mice for 33 days. Results revealed that berberine treatments markedly improve gut microbiota dysbiosis by increasing the abundance of Akkermansia muciniphila [32]. More recently, Han et al. investigated the protective effect of cornel iridoid glycoside isolated from Cornus officinalis Sieb. et Zucc against ALD. The cornel iridoid glycoside was supplemented at the dosage of 50, 100, and 200 mg/kg bw for 16 days in the mice. The results revealed that cornel iridoid glycoside supplementation significantly attenuated ALD via enhancing antioxidant activities, reducing inflammation, and altering intestinal microbial diversity. Cornel iridoid glycoside supplementation increased the abundance of Lactobacillus and decreased the proportion of norank_f_Muribaculaceae and norank_f_Desulfovibrionaceae in mice [70]. Furthermore, ursolic acid and antrodin A have recently been reported in two different studies to protect against alcohol-induced liver injury via the gut-liver axis [71,72].
Polysaccharides are polymeric carbohydrate molecules abundantly present in various plants, algae, microorganisms, and animals, exerting a wide array of biological activities, including hepatoprotective activities [73]. Many authors reported that polysaccharides could protect the liver from alcohol damage via multiple pathways, including restoring gut dysbiosis [32,[74][75][76]. In detail, Wang et al. isolated polysaccharides from garlic (molecular weight: 10 Kda, acid heteropolysaccharide), which was further studied against ALD in mice. Results showed that daily garlic polysaccharide administration (150 and 250 mg/kg bw for 30 days) could alleviate various biochemical indicators, increasing the abundance of Lachnospiraceae and Lactobacillus, and decreasing the abundance of Facklamia and Firmicutes in ethanol-induced mice [74]. Yang et al. found that inulin administration could ameliorate ALD via inhibiting the LPS-TLR4-Mψ axis, and rectified gut dysbiosis mainly by increasing the abundance of Lactobacillus, Lactococcus, and Allobaculum and reducing the abundance of Parasutterella [75]. In another study, Coprinus comatus polysaccharides could regulate gut microbiota in ALD mice by increasing the proportion of Lachnospiraceae, Firmicutes, Muribaculaceae, and Bacteroidetes and decreasing the Rikenellaceae proportion, which showed prebiotic-like effects on the intestinal flora in ALD mice [32]. Similarly, Wolfiporia cocos polysaccharides were also reported to modulate gut microbiota in ALD mice, mainly by increasing the Firmicutes to Proteobacteria ratio and the abundance of Lachnospiraceae [77]. Oral administration of oyster (Crassostrea gigas) polysaccharides (282 mg/kg bw) could also increase the proportion of Roseburia spp. and Lactobacillus reuteri, and decrease Escherichia proportion in ALD mice [78].

Fruits and Vegetables
Lychee (Litchi chinensis Sonn.) pulp extract rich in polyphenolic compounds (procyanidin B2, (-)-epicatechin, quercetin-3-O-rutinoside-7-O-α-L-rhamnosidase, rutin, and isorhamnetin-3-O-rutinoside) was orally given (0.2 and 0.4 g/L bw) to the ethanol-exposed (4%, v/v) mice for 8 weeks. Results revealed that compared with the ethanol group, lychee pulp extract supplementation increased the relative abundance of the Lactobacillus genus, Bacteroides acidifaciens species, Actinobacteria phylum, and Coriobacteriaceae family, whereas it decreased the abundance of Dehalobacteriaceae family and Odoribacter genus. Furthermore, it was also observed that lychee pulp extract supplementation could upregulate the expression of intestinal tight junction proteins, antimicrobial proteins, and mucus proteins while declining the serum endotoxin level. They concluded that lychee pulp extract has strong potential against alcoholic abuse [79]. In another study, pomegranate extract could also prevent intestinal apoptosis, endotoxemia, alcohol-induced intestinal leakage, and inflammation by regulating TJ/ adherent junction proteins [80]. Corchorus olitorius L., also known as molokhia, is a pantropical plant consumed as a vegetable in Africa and Eastern Asia that exerts a protective effect against several diseases [76,81]. Recently, Do et al. documented that administration of molokhia extract (50 and 100 mg/kg bw) restored the composition of Muribaculum and enhanced the intestinal barrier function in mice [76].

Cereals
Tang et al. documented that oats supplementation (10 g/kg bw) for 12 weeks to the rats could prevent alcohol-induced intestinal leakage by protecting the integrity of tight junctions and colonic mucosa [82]. Similarly, supplementation with rice bran phenolic extract was also reported to combat alcohol-induced liver injury via alleviating intestinal microbiota dysbiosis. Briefly, rice bran phenolic extract supplementation increased the abundance of Bacteroides acidifaciens and Lactobacillus, whereas it decreased pathogenic bacteria such as Muribaculum. Furthermore, it was also observed that rice bran phenolic extract could protect the intestinal barrier from alcohol [83]. Recently, Yang et al. reported that wheat embryo globulin could maintain the composition of gut microbiota [84].

Oils
Fish oil contains a significant amount of n-3 polyunsaturated fatty acids (PUFAs), which have been reported to alter gut microbiota. It has also been documented that fish oil supplementation can increase Bifidobacterium and decrease Escherichia coli in the feces of rats fed alcohol [85][86][87]. More recently, Chen et al. also reported that fish oil supplementation reduced the overgrowth of Rikenellaceae, Bacteroidetes, Alistipes, and Bacillaceae, inhibited endotoxin production, and suppressed TLR4 activation in chronic ethanol-fed rats [30].
Flaxseed oil was also reported to protect against the adverse effect of alcohol by modulating gut microbiota in alcohol-induced liver injury mice [88]. According to the report, Decaisnea insignis seed oil (containing palmitoleic acid, palmitic acid, and oleic acid) could protect against alcohol-associated liver damage in mice via increasing the abundance of Lactobacillus, Ruminoccoceae_UCG_004 and decreasing Parabacteroides abundance [89].
Okra seed oil supplementation has been reported to improve ALD via regulating intestinal microbiota. Briefly, Okra seed oil supplementation at the dosage of 400 and Antioxidants 2022, 11, 1211 7 of 18 800 mg/kg bw for 8 weeks decreased the proportion of Proteobacteria, Clostridium XlVa, and Staphylococcus, while enhancing the abundance of Bacteroidetes in alcohol-treated mice [90].

Tea
It has been reported that Pu-erh tea extract (PTE) played a protective role against ALD mainly through improving OS, lipid accumulation, inflammation, and microbiota dysbiosis. PTE treatment increased the relative abundance of potentially beneficial bacteria (Bifidobacterium and Allobaculum) and decreased the relative abundance of harmful bacteria (Helicobacter and Bacteroides) [91]. More recently, Li et al. studied the effects of six tea samples: two black teas (Dianhong tea and Yingde Black tea), two oolong teas (Tieguanyin Tea and Fenghuang Danzong Tea), and two dark teas (Fuzhuan Brick tea and Selenium-Enriched Dark tea) against ALD in mice. Results revealed that all tea sample supplementation markedly protected from the adverse effect of alcohol. However, more profound results were observed in oolong tea and dark tea. Moreover, their findings suggested that Akkermansia is the target microorganism for Tieguanyin Tea and Fu Brick Tea [92].

Fermented Liquids
Vinegar is fermented acidic food rich in various bioactive compounds such as polyphenols, flavonoids, and melanoidins. It was previously reported that Shanxi aged vinegar extract (SAVE) contains chlorogenic acid, p-hydroxybenzoic acid, ferulic acid, rutin, syringic acid, gallic acid, and other polyphenols, which exerts high antioxidant activity and protects liver cells from oxidative damage [83]. In another study, the same research group documented that polyphenol-rich SAVE attenuated ALD via regulating gut microbiota. Briefly, they found that various microbes (Lactobacillus, Bacteroidetes, Akkermansia, Verrucomicrobia) showed a significant positive correlation with OS and inflammatory indictors (occludin, Reg3b, Reg3g, and ZO-1), whereas Proteobacteria, Parabacteroides, Firmicutes, Bilophila, and Butyricimonas exhibited the opposite effect [93]. In addition, this research group also investigated the protective effect of another vinegar (Zhenjiang aromatic vinegar; ZAV) against ethanol-induced liver injury. Results showed that ZAV could regulate the composition of gut microbiota and immune factors in ALD mice. Additionally, Lach-nospiraceae_NK4A136_group, Bacteroidetes, and Akkermansia were positively correlated with antimicrobial peptides and intestinal immune factors, but negatively correlated with inflammatory and OS parameters [94].
Fermented rice liquors (called Makgeolli in Korea) were also documented to restore fecal microbiota compositions in mice induced by alcohol. The abundance of Bacteroidetes and Firmicutes phyla was observed to return to the control group level. Moreover, treatment with fermented rice liquors also increased the content of fecal SCFA and reduced inflammatory responses in mice induced by alcohol [95]. Baijiu, a Chinese traditional fermented liquor containing volatile compounds such as esters, acids, and phenols, was also reported to increase the relative abundance (11%) of Lactobacillus compared to the ethanol-treated group (1.80%) [96].
Ran et al. studied the protective effect of sea buckthorn-fermented liquid against ALD. The sea buckthorn was fermented with a Lactobacillus plantarum BNCC194165 strain, exhibiting a significant increment in the total flavonoids, total triterpenes, and SCFAs compared with the unfermented sea buckthorn. Furthermore, fermented sea buckthorn liquid was sterilized and orally given (1.75, 2.675, and 5.35 g/kg bw) to the mice for 15 days. Results showed that fermented sea buckthorn liquid could protect the liver from alcohol by improving OS, decreasing inflammation, and regulating gut microbiota. The high dosage of fermented sea buckthorn liquid (5.35 g/kg bw) significantly enhanced the abundance of Lactobacillus and decreased the abundance of Ruminiclostridium, Akkermansia, Alistipes, and Turicibacter in mice [97].

Herbs and Miscellaneous Extracts
Rhubarb (Rheum palmatum and Rheum officinale), a natural edible herb, contains a variety of bioactive compounds, including anthraquinone derivatives with hepatoprotective effects [98]. Neyrinck et al. reported that rhubarb extract (0.3%) could change the microbial composition of Akkermansia muciniphila and Parabacteroides goldsteinii, improving hepatic injury and decreasing inflammatory and OS biomarkers in alcohol-induced mice [99].
Choi et al. conducted a study to explore the protective effect of defatted Tenebrio molitor larva fermented extract against chronic alcohol-fed rats. Results showed that defatted Tenebrio molitor larva fermented with Saccharomyces cerevisiae strain (KCTC 17299) extract at the dosage of 200 mg/kg/day could attenuate ALD via modulating intestinal microflora, steatosis, and inflammation. It was observed that defatted Tenebrio molitor larva extract restored the Lactobacillus johnsonii abundance [102]. Similarly, many other natural extracts such as Pogostemon cablin, edible insect Gryllus bimaculatus, Semen Hoveniae, and Dendropanax morbifera leaf extract were reported to protect against alcohol-mediated gut microbiota dysbiosis [103][104][105][106].

Discussion
A large amount of evidence suggests that intestinal microbiome dysregulation is a key risk factor for the progression/development of ALD. The graphical summary of the effects of chronic alcohol consumption on the gut-liver axis is shown in Figure 2. Prevention of alcohol-induced dysbiosis is an important strategy in the treatment of ALD. In this regard, natural products and bioactive compounds play a significant role. As mentioned above, edible plants and their bioactive compounds could restore gut microbiota in animal models (Table 1). [76] Oats Not investigated Male SD rats were gavaged for 12 weeks with alcohol (starting dose of 1 g/kg increasing to 6 g/kg/day over the first 2 weeks) or dextrose, with or without oats supplementation (10 g/kg/day).
Rice bran phenolic extract supplementation increased the Bacteroides acidifaciens and Lactobacillus population while decreasing pathogenic bacteria such as Muribaculum. Rice bran phenolic extract could protect the intestinal barrier function from alcohol. [83] Fish oil Not investigated Thirty-six male Wistar rats (8 weeks old) were divided into six groups: control, control diet with 25% fish oil substitution, control diet with 57% fish oil substitution, ethanol-containing diet, an ethanol-containing diet with 25% fish oil substitution, and ethanol-containing diet with 57% fish oil substitution groups.
Okra seed oil supplementation decreased the proportion of Proteobacteria, Clostridium XlVa, and Staphylococcus, while enhancing the Bacteroidetes population in alcohol-treated mice. [90]

Extract Bioactive Compound Study Design Major Finding Ref
Pu PTE treatment increased the relative abundance of potentially beneficial bacteria (Bifidobacterium and Allobaculum) and decreased the relative abundance of harmful bacteria (Helicobacter and Bacteroides). [91] Green tea Oyster polysaccharides (282 mg/kg bw) were orally given to mice.
Oral administration of oyster (Crassostrea gigas) polysaccharides (282 mg/kg bw) could also increase the proportion of Roseburia spp. and Lactobacillus reuteri, and decrease Escherichia proportion in ALD mice. [78] Antioxidants 2022, 11, 1211 9 of Figure 2. Summary of the effects of chronic alcohol consumption on the gut-liver axis.  Briefly, Lactobacillus, a therapeutically relevant bacterial genus, decreased after alcohol intake, which can be further restored through various bioactive compounds and natural products [70,74,78,79,96,97]. Lactobacillus is a beneficial bacteria that produces bacteriocins such as antibiotics, which further inhibit harmful microbes of the Enterobacteriaceae family, such as Salmonella or Shigella [107]. Lactobacillus can protect against pathogenic and invasive bacteria by adhering to intestinal epithelial cells [108,109]. In addition, they produce SCFAs (lactic acid, propionic acid, or butyric acid), which provide nutrition to epithelial cells [110].
Allobaculum and Bifidobacterium are beneficial intestinal bacteria that produce SC-FAs (butyric and lactic acids) and a small amount of ethanol from glucose. In addition, Bifidobacterium is a potential acetaldehyde accumulator [101]. Natural products and bioactive compounds increased the abundance of Allobaculum and Bifidobacterium in alcoholexposed mice [91].
The Bacteroidetes phylum is composed of three major classes of gram-negative bacteria present in the intestines, upper respiratory tract, mouth, and genital tracts of animals and humans, exerting both beneficial and harmful functions. Bacteroidetes may lead to endogenous infections due to microecological imbalance. It has been documented that Bacteroides fragilis can produce polysaccharide A to relieve colitis in animals [111]. Conversely, it also produces toxins, which facilitate pro-carcinogenic effects and mediate colon tumorigenesis [112]. Moreover, the growth of Proteobacteri (pro-inflammatory intestinal microbes) increased due to imbalanced microbial composition, linked with the occurrence and development of disease [113]. Natural products and bioactive compounds were reported to decrease the abundance of Bacteroidetes and Proteobacteria in alcohol-exposed animals [31].
Several studies have documented the beneficial effects of Akkermansia on host metabolites. In the phylum Verrucomicrobia, Akkermansia is a dominant genus that interferes with intestinal mucin, enhances gut barrier function, increases mucus thickness, and inversely correlates with metabolic syndrome and inflammation [114][115][116]. Akkermansia deficiency is an early sign of alcoholic gut dysbiosis [117]. Furthermore, alcohol exposure reduced the population of Akkermansia in both mice and humans, and alcoholic fatty liver disease (AFLD) can be improved by supplementation of the genus, indicating the protective role of this bacterium against AFLD [118]. Due to the nature of probiotics, the abundance of Akkermansia may be affected by dietary ingredient supplementation [119]. On the other hand, the abundance of Staphylococcus was directly related to the expression of TNF-α in the liver of ALD, and the overabundance of Staphylococcus in the gut may be linked with the aggravation of hepatic inflammation [120]. Bioactive compounds could decrease the Helicobacter abundance, a key marker in patients with gastric disease multiplying and growing in the intestine, slowing its production of a huge amount of endotoxin in the gut [121,122].
Although many studies support that natural products and bioactive compounds could modulate gut microbiota in animal experiments, there are still some limitations in current studies, such as that (a) some plant-based functional food extracts are reported to exert a protective effect against ALD dysbiosis. However, their bioactive components have not yet been characterized. (b) the dosage needs to be optimized to avoid adverse effects and contribute to the beneficial effects of plant-based functional foods, (c) effects of plant-based functional foods and their bioactive components are mostly investigated based on animal models, which lacks the in-depth systematic analyses, (d) large-scale clinical trials investigating the role of plant-based functional foods and their bioactive components against ALD have not been conducted as results based on animal models and humans may differ. Despite limitations and gaps, plant-based functional foods and their bioactive components are a viable approach for treating ALD dysbiosis.

Conclusions
ALD is a disease caused by excessive consumption of alcohol with high morbidity and mortality worldwide. Gut microbiota plays a key role in many metabolic processes beneficial to the host, such as the production of SCFAs and vitamins. However, excessive intake of alcohol is also associated with gut dysbiosis in the pathogenesis of ALD. Natural products and phytochemicals are important sources of novel therapeutic agents against chronic diseases, including ALD. Natural products and related phytochemicals act through multiple pathways, such as modulating gut microbiota, improving redox stress, and anti-inflammation. Natural products and phytochemicals can increase the relative abundance of beneficial microbes (Lactobacillus, Bacteroides acidifaciens, Actinobacteria, Coriobacteriaceae, Akkermansia, Verrucomicrobia, etc.) and decrease the relative abundance of harmful microbes (Bacteroidetes, Proteobacteria, Parabacteroides, Butyricimonas, Bilophila, etc.), indicating the protective effects against ALD. Natural products could also prevent intestinal apoptosis, endotoxemia, alcohol-induced intestinal leakage, and inflammation by regulating TJ/adherent junction proteins, LPS-TLR4 pathway, and OS biomarkers, thereby protecting against ALD. Based on animal studies, natural products and related phytochemicals have proved ideal candidates for combating ALD and its complications. Notably, the transferability of these findings might not yet be possible because clinical trials are still warranted.