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Article

Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells

1
Department of Psychiatry, Yuli Branch, Taipei Veterans General Hospital, Hualien 98142, Taiwan
2
Department of Future Studies and LOHAS Industry, Fo Guang University, Jiaosi, Yilan County 26247, Taiwan
3
Department of Psychiatry, Mackay Medical College, New Taipei City 25245, Taiwan
4
Department of Psychiatry, Taitung Mackay Memorial Hospital, Taitung County 95064, Taiwan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Brain Sci. 2020, 10(8), 545; https://doi.org/10.3390/brainsci10080545
Received: 10 July 2020 / Revised: 7 August 2020 / Accepted: 8 August 2020 / Published: 12 August 2020
(This article belongs to the Section Molecular and Cellular Neuroscience)
Valproic acid (VPA) is a multifunctional medication used for the treatment of epilepsy, mania associated with bipolar disorder, and migraine. The pharmacological effects of VPA involve a variety of neurotransmitter and cell signaling systems, but the molecular mechanisms underlying its clinical efficacy is to date largely unknown. In this study, we used the isobaric tags for relative and absolute quantitation shotgun proteomic analysis to screen differentially expressed proteins in VPA-treated SH-SY5Y cells. We identified changes in the expression levels of multiple proteins involved in Alzheimer’s disease, Parkinson’s disease, chromatin remodeling, controlling gene expression via the vitamin D receptor, ribosome biogenesis, ubiquitin-mediated proteolysis, and the mitochondrial oxidative phosphorylation and electron transport chain. Our data indicate that VPA may modulate the differential expression of proteins involved in mitochondrial function and vitamin D receptor-mediated chromatin transcriptional regulation and proteins implicated in the pathogenesis of neurodegenerative diseases. View Full-Text
Keywords: valproic acid; proteomics; epigenetics; pathway analysis valproic acid; proteomics; epigenetics; pathway analysis
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MDPI and ACS Style

Hu, T.-M.; Chung, H.-S.; Ping, L.-Y.; Hsu, S.-H.; Tsai, H.-Y.; Chen, S.-J.; Cheng, M.-C. Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells. Brain Sci. 2020, 10, 545. https://doi.org/10.3390/brainsci10080545

AMA Style

Hu T-M, Chung H-S, Ping L-Y, Hsu S-H, Tsai H-Y, Chen S-J, Cheng M-C. Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells. Brain Sciences. 2020; 10(8):545. https://doi.org/10.3390/brainsci10080545

Chicago/Turabian Style

Hu, Tsung-Ming, Hsiang-Sheng Chung, Lieh-Yung Ping, Shih-Hsin Hsu, Hsin-Yao Tsai, Shaw-Ji Chen, and Min-Chih Cheng. 2020. "Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells" Brain Sciences 10, no. 8: 545. https://doi.org/10.3390/brainsci10080545

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