An Investigation of the Effects of Changes in the Indoor Ambient Temperature on Arousal Level , Thermal Comfort , and Physiological Indices †

Thermal factors not only affect the thermal comfort sensation of occupants, but also affect their arousal level, productivity, and health. Therefore, it is necessary to control thermal factors appropriately. In this study, we aim to design a thermal environment that improves both the arousal level and thermal comfort of the occupants. To this end, we investigated the relationships between the physiological indices, subjective evaluation values, and task performance under several conditions of changes in the indoor ambient temperature. In particular, we asked subjects to perform a mathematical task and subjective evaluation related to their thermal comfort sensation and drowsiness levels. Simultaneously, we measured their physiological parameters, such as skin temperature, respiration rate, electroencephalography, and electrocardiography, continuously. We investigated the relationship between the comfort sensation and drowsiness level of occupants, and the physiological indices. From the results, it was confirmed that changes in the indoor ambient temperature can improve both the thermal comfort and the arousal levels of occupants. Moreover, we proposed the evaluation indices of the thermal comfort and the drowsiness level of occupants using physiological indices.


Introduction
The control of indoor environmental quality (IEQ, which consists of visual elements, olfactory elements, and thermal factors, etc.) is important for improvements of the comfort and productivity of occupants.Among the factors of IEQ, thermal factors, such as ambient temperature, radiant temperature, humidity, and air velocity, are especially related with not only the thermal sensation and thermal comfort of occupants, but also productivity and health.With the high technology of heating, ventilation, and air conditioning systems (HVAC systems), the relationship between thermal factors and thermal sensation and thermal comfort has received attention from several researchers worldwide.In previous research, indices of thermal comfort, such as the predicted mean vote (PMV), predicted percent dissatisfied (PPD), and standard new effective temperature (SET*), have been proposed based on the relationship between thermal factors and the subjective evaluation of thermal comfort sensation [1,2].Many studies have analyzed the indoor thermal quality based on the evaluation of thermal factors using these indices [3][4][5][6][7].
In addition, improvements of the productivity of occupants who are working, studying, and driving in indoor spaces, such as offices, classrooms, and vehicles, are needed.It is necessary to maintain a high arousal state of the occupants to improve their productivity.In previous studies, it was shown that productivity improved when the arousal level of occupants was high, and this was related to the indoor ambient temperature that occupants felt as cool or cold [8,9].From the results of previous studies, the conditions of the ambient temperature to improve task performance were different from the conditions of the ASHRAE (American Society of Heating, Refrigerating and Air-Conditioning Engineers)'s thermal comfort zone [10].Therefore, the conditions of the constant ambient temperature were not appropriate to improve both the task performance and thermal comfort levels.In previous studies, it was shown that the thermal comfort of occupants was immediately changed in accordance with changes in the indoor ambient temperature [11].The arousal level was increased by outer stimulation and was maintained at a high state for several minutes [12,13].Based on these results of previous studies, we hypothesized that thermal stimulation due to cooling can improve the arousal level of occupants.Furthermore, thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation.When considering the possibility that changes in thermal factors can improve both arousal and thermal comfort levels, the physiological effects associated with such changes in thermal factors, and how they affect the arousal level and thermal comfort of occupants are not clear.Therefore, the design requirements for changes in ambient temperature to improve both the arousal level and thermal comfort are also not clear.Therefore, a continuous and quantitative evaluation of the thermal comfort and arousal level of occupants using indices, which can be measured both continuously and quantitatively, such as physiological signals, is needed to clarify the design requirements of changes in the indoor ambient temperature to improve both the arousal level and thermal comfort.
In this study, we aimed to investigate the characteristics of changes in the arousal level and feelings of thermal comfort of occupants, and the relationship between them when thermal factors are changed.There are many factors that affect the thermal comfort and arousal level of occupants, such as the ambient temperature, indoor air velocity, mean air radiant temperature, and metabolic activity [5][6][7].Especially, we focused on the changes in the indoor ambient temperature as a fundamental investigation in this study.In addition, to propose evaluation indices that can evaluate the thermal comfort and arousal level of occupants continuously and quantitatively, we investigated the relationship between the arousal level, feelings of thermal comfort, and physiological indices, which can be measured continuously and quantitatively.

Investigation of the Effects of Changes in the Indoor Ambient Temperature on Arousal Levels, Thermal Comfort, and Task Performance
To verify the hypothesis that thermal stimulation, due to cooling, can improve the arousal level of occupants, and that thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation, several thermal conditions were set.Subjects were asked to conduct a mathematical task and periodically evaluate their sensation values (using a subjective sensation vote) in relation to their arousal level and thermal comfort.We attempt to clarify the effect of changes in the indoor ambient temperature on the arousal level, thermal comfort, and task performance of occupants by conducting an analysis of the results of the subjective sensation vote and task performance.

Investigation of the Relation between the Subjective Evaluation Value and Physiological Parameters, and a Recommendation for Evaluation Indices
Indices that can evaluate the arousal and thermal comfort levels of occupants continuously and quantitatively are necessary to clarify the design requirements of a temperature control that can improve the arousal and thermal comfort levels of occupants.However, as it was not possible to perform a subjective sensation voting continuously, we considered clarifying the design requirements for thermal environments using changes over time.This relied on the possibility to evaluate the arousal level and feelings of thermal comfort using physiological parameters that could be measured quantitatively and continuously.Previous studies have shown that the physiological indices measured utilizing electroencephalograms (EEG), electrocardiograms (ECG), and respiration rates were effective for the evaluation of arousal levels [14,15], and the skin temperature, EEGs, and ECGs of occupants were effective for the evaluation of the thermal comfort [16,17].Therefore, in this study, we assumed a flow of physiological responses when the occupant was stimulated by thermal factors, as shown in Figure 1, based on the above-mentioned previous studies to clarify the characteristics of the physiological parameters under the condition of changes in both the arousal level and thermal comfort.A thermal stimulation is transmitted from the sensory organ, such as warm spots and cold spots of skin, to the central nervous system, which affects thermal and comfort sensation.This change in comfort sensation then affects the autonomic nervous system, and the response is then conducted through the locomotive organ.Based on this process, we selected the skin temperature as an indicator of heat transfer between the environment and the human body, EEGs as an indicator of the reaction of the central nervous system, and ECGs as indicators of the reaction of the autonomic nervous system.Next, we attempted to search for indices that could separately evaluate the arousal level and thermal comfort by performing a multiple regression analysis utilizing the physiological parameters and subjective evaluation values of the arousal level and feelings of thermal comfort.In Figure 1, the flow of physiological responses with the thermal stimulus is shown.improve the arousal and thermal comfort levels of occupants.However, as it was not possible to perform a subjective sensation voting continuously, we considered clarifying the design requirements for thermal environments using changes over time.This relied on the possibility to evaluate the arousal level and feelings of thermal comfort using physiological parameters that could be measured quantitatively and continuously.Previous studies have shown that the physiological indices measured utilizing electroencephalograms (EEG), electrocardiograms (ECG), and respiration rates were effective for the evaluation of arousal levels [14,15], and the skin temperature, EEGs, and ECGs of occupants were effective for the evaluation of the thermal comfort [16,17].Therefore, in this study, we assumed a flow of physiological responses when the occupant was stimulated by thermal factors, as shown in Figure 1, based on the above-mentioned previous studies to clarify the characteristics of the physiological parameters under the condition of changes in both the arousal level and thermal comfort.A thermal stimulation is transmitted from the sensory organ, such as warm spots and cold spots of skin, to the central nervous system, which affects thermal and comfort sensation.This change in comfort sensation then affects the autonomic nervous system, and the response is then conducted through the locomotive organ.Based on this process, we selected the skin temperature as an indicator of heat transfer between the environment and the human body, EEGs as an indicator of the reaction of the central nervous system, and ECGs as indicators of the reaction of the autonomic nervous system.Next, we attempted to search for indices that could separately evaluate the arousal level and thermal comfort by performing a multiple regression analysis utilizing the physiological parameters and subjective evaluation values of the arousal level and feelings of thermal comfort.In Figure 1, the flow of physiological responses with the thermal stimulus is shown.

Subjects
Ten subjects with vital statistics of a height of 173.5 ± 4.4 cm, weight of 61 ± 4.8 kg, age of 22.1 ± 1.2 years old, and who were right-handed participated in the experiment.The experimental contents and procedures, which were approved by the ethics committee of the University of Tokyo, were explained to the subjects before conducting the experiment.The subjects were then asked to avoid intense physical activity, alcohol, and caffeine for 24 h prior to the experimental session.

Experimental Task
The subjects were asked to conduct mental arithmetic tasks known as "MATH", which is based on the algorithm proposed by Tuner et al. [18].A 1-3-digit addition or subtraction question is displayed for 2 s on the monitor, and then "equals to" is displayed for 1.5 s.Lastly, the answer is displayed for 1 s, and the next question is displayed after 0.5 s.Subjects had to determine if the answer was correct or incorrect when the answer was displayed and click the left mouse button if the answer was correct or the right mouse button if the answer was incorrect.The levels of questions in the original version of MATH consisted of 1-5 levels.In this study, the beginning level was 3 (which is 2-digit addition or subtraction), and the level of the next question was raised if the responses of the subjects were correct, and was reduced if answers were incorrect.We deduced that changing the levels affected the physiological indices.Thus, we did not change the level of the question, and fixed it to level 3.The MATH task included 50 questions for 250 seconds per set.

Subjects
Ten subjects with vital statistics of a height of 173.5 ± 4.4 cm, weight of 61 ± 4.8 kg, age of 22.1 ± 1.2 years old, and who were right-handed participated in the experiment.The experimental contents and procedures, which were approved by the ethics committee of the University of Tokyo, were explained to the subjects before conducting the experiment.The subjects were then asked to avoid intense physical activity, alcohol, and caffeine for 24 h prior to the experimental session.

Experimental Task
The subjects were asked to conduct mental arithmetic tasks known as "MATH", which is based on the algorithm proposed by Tuner et al. [18].A 1-3-digit addition or subtraction question is displayed for 2 s on the monitor, and then "equals to" is displayed for 1.5 s.Lastly, the answer is displayed for 1 s, and the next question is displayed after 0.5 s.Subjects had to determine if the answer was correct or incorrect when the answer was displayed and click the left mouse button if the answer was correct or the right mouse button if the answer was incorrect.The levels of questions in the original version of MATH consisted of 1-5 levels.In this study, the beginning level was 3 (which is 2-digit addition or subtraction), and the level of the next question was raised if the responses of the subjects were correct, and was reduced if answers were incorrect.We deduced that changing the levels affected the physiological indices.Thus, we did not change the level of the question, and fixed it to level 3.The MATH task included 50 questions for 250 seconds per set.

Experimental Conditions and Experimental Procedure
To evaluate the effects of the duration and degree of thermal stimulation, three environmental conditions (A-C) were set as follows.

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Condition A: The indoor ambient temperature was maintained at 27 We set condition A as a thermally comfort condition [10], and condition C as a high arousal condition [8,9].Condition B was set to verify the hypothesis that thermal stimulation due to cooling can improve the arousal levels of occupants, after which thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation.There are many parameters which affect the thermal comfort and arousal levels of occupants, such as the indoor velocity, mean air radiant temperature, metabolic activity, and amount of clothing.In this study, we focused on only the indoor ambient temperature as a fundamental investigation, thus those parameters except the indoor ambient temperature were controlled in the experiment.The subjects wore short sleeves and short pants and were asked to remain in the pre-room.The room temperature was set at 27 ± 0.5 • C for approximately 1 h so that subjects could adjust to the thermal environment; they were asked to practice the "MATH" at least twice during this time.Sensors were then attached to the bodies of the subjects to measure their physiological indices.One set of tasks consisted of completing the subjective sensation vote and the "MATH" task.The subjects were asked to perform seven sets of tasks at time intervals of 10 min and to rest between each task for approximately 3 min.Physiological indices were measured from the start time (0 min) till the end time (70 min).The experimental procedure and environmental conditions are shown in Figure 2.

Experimental Conditions and Experimental Procedure
To evaluate the effects of the duration and degree of thermal stimulation, three environmental conditions (A-C) were set as follows.We set condition A as a thermally comfort condition [10], and condition C as a high arousal condition [8,9].Condition B was set to verify the hypothesis that thermal stimulation due to cooling can improve the arousal levels of occupants, after which thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation.There are many parameters which affect the thermal comfort and arousal levels of occupants, such as the indoor velocity, mean air radiant temperature, metabolic activity, and amount of clothing.In this study, we focused on only the indoor ambient temperature as a fundamental investigation, thus those parameters except the indoor ambient temperature were controlled in the experiment.The subjects wore short sleeves and short pants and were asked to remain in the pre-room.The room temperature was set at 27 ± 0.5 °C for approximately 1 h so that subjects could adjust to the thermal environment; they were asked to practice the "MATH" at least twice during this time.Sensors were then attached to the bodies of the subjects to measure their physiological indices.One set of tasks consisted of completing the subjective sensation vote and the "MATH" task.The subjects were asked to perform seven sets of tasks at time intervals of 10 min and to rest between each task for approximately 3 min.Physiological indices were measured from the start time (0 min) till the end time (70 min).The experimental procedure and environmental conditions are shown in Figure 2.  Before completing the "MATH" task, the subjects were asked to complete a questionnaire related to their thermal sensation, thermal comfort sensation, and arousal level.The scale of thermal sensation was based on ASHRAE/ISO (International Organization for Standardization) [10], and was denoted using integral numbers from −3 to 3 (where −3, −2, −1, 0, 1, 2, and 3 are the meanings of cold, cool, slightly cool, neutral, slightly warm, warm, and hot, respectively.).The scale of comfort sensation was based on ISO10551 [19], and was denoted using integral numbers from −3 to 0 (where −3, −2, −1, and 0 are the meanings of very uncomfortable, uncomfortable, slightly uncomfortable, and comfortable, respectively.).The scale of the arousal level was based on the drowsiness level of Zilberg's indicators [20], and was denoted using integral numbers from 0 to 4 (where 0, 1, 2, 3, and 4 are the meanings of alert, slightly drowsy, moderately drowsy, significantly drowsy, and extremely drowsy, respectively.).Before completing the "MATH" task, the subjects were asked to complete a questionnaire related to their thermal sensation, thermal comfort sensation, and arousal level.The scale of thermal sensation was based on ASHRAE/ISO (International Organization for Standardization) [10], and was denoted using integral numbers from −3 to 3 (where −3, −2, −1, 0, 1, 2, and 3 are the meanings of cold, cool, slightly cool, neutral, slightly warm, warm, and hot, respectively.).The scale of comfort sensation was based on ISO10551 [19], and was denoted using integral numbers from −3 to 0 (where −3, −2, −1, and 0 are the meanings of very uncomfortable, uncomfortable, slightly uncomfortable, and comfortable, respectively.).The scale of the arousal level was based on the drowsiness level of Zilberg's indicators [20], and was denoted using integral numbers from 0 to 4 (where 0, 1, 2, 3, and 4 are the meanings of alert, slightly drowsy, moderately drowsy, significantly drowsy, and extremely drowsy, respectively.).
(b) ECG ECGs were recorded using an ECG-measuring instrument (WEB-7000 and ECG picker, Nihonkohden Co., Japan) at a sampling rate of 1000 Hz.Three electrodes were attached to the chests of subjects using the precordial leads method.The R-R interval (RRI) was calculated from the ECG waveform using MATLAB (Mathwork Co.) programs.The values of the mean of the RRI and the coefficient of variance of RRI [CVRR (100*SD/Mean of RRI)] were calculated from the RRI data.In addition, the spectral power of each frequency band, such as the very low frequency (VLF, 0.001-0.04Hz), low frequency (LF, 0.04-0.15Hz), and high frequency (HF, 0.15-0.45Hz) bands, was calculated from the time series of the RRI using the fast Fourier transform method.

(c) Respiration
A thermal picker (WEB-7000, Nihonkohden Co., Japan) was used to measure the temperature of the breath of subjects.The peak values in the time series graph of the temperature were detected, and the mean and standard deviation of the respiratory cycle time were calculated.

(d) Skin Temperature
Thermocouples were attached at 7 places on the bodies of subjects based on the Hardy-Du Bois method [16] to measure the skin temperature (LT8, GRAM Co., Japan).Finally, the mean skin temperature was calculated using following Equation (1): where MST is the mean skin temperature based on the Hardy-DuBois method [16], and T 1 , T 2 , T 3 , T 4 , T 5 , T 6 , and T 7 are the temperatures of the forehead, forearms, hands, abdomen, thighs, legs, and feet, respectively.

Facial Expression and Task Performance
The activities of subjects were recorded using the video camera, and the drowsiness levels were recorded by an observer at intervals of 10 s based on Zilberg's criteria [20].After the experiment was completed, the mean of the data recorded when subjects were performing the MATH task was calculated.The task performance of MATH included the number of correct answers and the mean of the reaction time taken to solve 50 questions.Figure 3 shows a participant completing the experiment.Figure 3 shows photos of the field of experimental scene.

. Subjective Evaluation Value
The results of the subjective evaluation value corresponding to the drowsiness levels and the thermal comfort sensation vote of subjects under conditions A, B, and C are shown in Figures 4 and  5.We used multiple comparison based on the Bonferroni method to investigate significant differences of the subjective evaluation value due to changes in the indoor ambient temperature.There was a small change in the comfort sensation vote, but the drowsiness level increased under condition A, where the indoor temperature was maintained at 27 °C.Under condition B, the comfort sensation vote decreased to an uncomfortable state when the indoor ambient temperature dropped, and increased to a comfortable state when the indoor ambient temperature increased.Under condition C, the comfort sensation vote decreased to an uncomfortable state when the indoor temperature decreased and was then maintained in this uncomfortable state.Under conditions B and C, the drowsiness levels decreased and were maintained at an alert state, corresponding to the drop in the indoor temperature.After the completion of set 4, the comfort sensation vote increased, corresponding to the increase in the indoor temperature under condition B, but was maintained at an uncomfortable state under condition C. The drowsiness level was maintained at a low state under both conditions.According to this result, the arousal level was maintained at a high state even when the temperature increased, and subjects felt comfortable even when the temperature dropped, leading to an increased arousal level.This suggests that there is not always a dependence relationship between the arousal levels and feelings of thermal comfort.Figures 4 and 5 show that when condition B is applied in sets 6 and 7, it becomes possible to improve both the arousal level and thermal comfort of occupants by changing the indoor ambient temperature.These results show that the hypothesis that thermal stimulation due to cooling can improve the arousal level of occupants, after which thermal comfort can also be improved while maintaining a high arousal levels due to the removal of thermal stimulation was verified for condition B. Figure 4 shows the result of the thermal comfort sensation vote.Figure 5 shows the result of the drowsiness level sensation vote.

. Subjective Evaluation Value
The results of the subjective evaluation value corresponding to the drowsiness levels and the thermal comfort sensation vote of subjects under conditions A, B, and C are shown in Figures 4 and 5.We used multiple comparison based on the Bonferroni method to investigate significant differences of the subjective evaluation value due to changes in the indoor ambient temperature.There was a small change in the comfort sensation vote, but the drowsiness level increased under condition A, where the indoor temperature was maintained at 27 • C.Under condition B, the comfort sensation vote decreased to an uncomfortable state when the indoor ambient temperature dropped, and increased to a comfortable state when the indoor ambient temperature increased.Under condition C, the comfort sensation vote decreased to an uncomfortable state when the indoor temperature decreased and was then maintained in this uncomfortable state.Under conditions B and C, the drowsiness levels decreased and were maintained at an alert state, corresponding to the drop in the indoor temperature.After the completion of set 4, the comfort sensation vote increased, corresponding to the increase in the indoor temperature under condition B, but was maintained at an uncomfortable state under condition C. The drowsiness level was maintained at a low state under both conditions.According to this result, the arousal level was maintained at a high state even when the temperature increased, and subjects felt comfortable even when the temperature dropped, leading to an increased arousal level.This suggests that there is not always a dependence relationship between the arousal levels and feelings of thermal comfort.Figures 4 and 5 show that when condition B is applied in sets 6 and 7, it becomes possible to improve both the arousal level and thermal comfort of occupants by changing the indoor ambient temperature.These results show that the hypothesis that thermal stimulation due to cooling can improve the arousal level of occupants, after which thermal comfort can also be improved while maintaining a high arousal levels due to the removal of thermal stimulation was verified for condition B. Figure 4 shows the result of the thermal comfort sensation vote.Figure 5 shows the result of the drowsiness level sensation vote.The rating values of Zilberg's drowsiness level for each condition are shown in Figure 6, and it is evident from the figure that the results are similar to those of the subjective sensation vote.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences of the drowsiness level due to the changes in the indoor ambient temperature.After the completion of set 3, the drowsiness levels under conditions B and C decreased significantly compared to that under condition A. From the result of condition B, it was confirmed that the drowsiness level was maintained at a low state after the completion of set 3.

Task Performance of MATH
The results of the MATH score under each condition are shown in Figure 7, and the results of the response time taken to calculate MATH questions under each condition are shown in Figure 8.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences in the task performance due to changes in the indoor ambient temperature.It can be seen from Figures 7 and 8 that the MATH score was high after set 4, and the response time taken to calculate MATH questions increases in the order of conditions C, B, and then A. Differences were observed between the conditions in the results of the subjective sensation vote and the rating value of Zilberg's drowsiness level.However, significant differences were observed only between the conditions in the result of the response time taken to calculate MATH for set 4, and the result of the MATH score for set 5. It was assumed that the MATH work was easy enough for the subjects to perform, even at a low state of arousal.Figure 6 shows the drowsiness level reached when calculating MATH (analysis of facial expressions based on Zilberg's method [20]).Figure 7 and Figure 8 show the result of the MATH score and the response time from the display of the MATH question to the participant's click.

Rating Value of Zilberg's Drowsiness Level by Observer
The rating values of Zilberg's drowsiness level for each condition are shown in Figure 6, and it is evident from the figure that the results are similar to those of the subjective sensation vote.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences of the drowsiness level due to the changes in the indoor ambient temperature.After the completion of set 3, the drowsiness levels under conditions B and C decreased significantly compared to that under condition A. From the result of condition B, it was confirmed that the drowsiness level was maintained at a low state after the completion of set 3.

Task Performance of MATH
The results of the MATH score under each condition are shown in Figure 7, and the results of the response time taken to calculate MATH questions under each condition are shown in Figure 8.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences in the task performance due to changes in the indoor ambient temperature.It can be seen from Figures 7 and 8 that the MATH score was high after set 4, and the response time taken to calculate MATH questions increases in the order of conditions C, B, and then A. Differences were observed between the conditions in the results of the subjective sensation vote and the rating value of Zilberg's drowsiness level.However, significant differences were observed only between the conditions in the result of the response time taken to calculate MATH for set 4, and the result of the MATH score for set 5. It was assumed that the MATH work was easy enough for the subjects to perform, even at a low state of arousal.Figure 6 shows the drowsiness level reached when calculating MATH (analysis of facial expressions based on Zilberg's method [20]).Figure 7 and Figure 8 show the result of the MATH score and the response time from the display of the MATH question to the participant's click.

Rating Value of Zilberg's Drowsiness Level by Observer
The rating values of Zilberg's drowsiness level for each condition are shown in Figure 6, and it is evident from the figure that the results are similar to those of the subjective sensation vote.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences of the drowsiness level due to the changes in the indoor ambient temperature.After the completion of set 3, the drowsiness levels under conditions B and C decreased significantly compared to that under condition A. From the result of condition B, it was confirmed that the drowsiness level was maintained at a low state after the completion of set 3.

Task Performance of MATH
The results of the MATH score under each condition are shown in Figure 7, and the results of the response time taken to calculate MATH questions under each condition are shown in Figure 8.Multiple comparison based on the Bonferroni method was conducted to investigate significant differences in the task performance due to changes in the indoor ambient temperature.It can be seen from Figures 7  and 8 that the MATH score was high after set 4, and the response time taken to calculate MATH questions increases in the order of conditions C, B, and then A. Differences were observed between the conditions in the results of the subjective sensation vote and the rating value of Zilberg's drowsiness level.However, significant differences were observed only between the conditions in the result of the response time taken to calculate MATH for set 4, and the result of the MATH score for set 5. It was assumed that the MATH work was easy enough for the subjects to perform, even at a low state of arousal.Figure 6 shows the drowsiness level reached when calculating MATH (analysis of facial expressions based on Zilberg's method [20]).Figures 7 and 8 show the result of the MATH score and the response time from the display of the MATH question to the participant's click.

Consideration of Thermal Stimulation for High Arousal Levels
In the previous studies of Yoshida et al. [12] and Mohri et al. [13], to maintain a high arousal level, the effects of several stimulation methods, such as the use of a fragrance, alarm, and shoulder oscillation, were confirmed.As a result, the sleeping rebound occurred 10 min after the stimulation in those studies.As in our results, high arousal states were maintained for over 10 min after the removal of thermal stimulation.Though further investigation of these comparisons of the time to rebound is necessary, our results showed that thermal stimulation is effective in improving the arousal levels of occupants.Especially, our method is useful in spaces where air conditioning systems

Consideration of Thermal Stimulation for High Arousal Levels
In the previous studies of Yoshida et al. [12] and Mohri et al. [13], to maintain a high arousal level, the effects of several stimulation methods, such as the use of a fragrance, alarm, and shoulder oscillation, were confirmed.As a result, the sleeping rebound occurred 10 min after the stimulation in those studies.As in our results, high arousal states were maintained for over 10 min after the removal of thermal stimulation.Though further investigation of these comparisons of the time to rebound is necessary, our results showed that thermal stimulation is effective in improving the arousal levels of occupants.Especially, our method is useful in spaces where air conditioning systems

Consideration of Thermal Stimulation for High Arousal Levels
In the previous studies of Yoshida et al. [12] and Mohri et al. [13], to maintain a high arousal level, the effects of several stimulation methods, such as the use of a fragrance, alarm, and shoulder oscillation, were confirmed.As a result, the sleeping rebound occurred 10 min after the stimulation in those studies.As in our results, high arousal states were maintained for over 10 min after the removal of thermal stimulation.Though further investigation of these comparisons of the time to rebound is necessary, our results showed that thermal stimulation is effective in improving the arousal levels of occupants.Especially, our method is useful in spaces where air conditioning systems are installed, because the installation of additional devices in the space to make stimulations, such as fragrances, alarms, and shoulder oscillation, is unnecessary.

Relationship between the Subjective Evaluation Value and Physiological Indices
Three hundred and twenty-nine physiological indices were calculated by using the data of the EEGs, ECGs, respiration rate, and mean skin temperature in the experiment (320 indices from EEGs, 7 indices from ECGs, 2 indices from respiration rate, and 1 index from skin temperature).By considering the result of the subjective evaluation value as the standard for the perception of state, we used correlative analysis to investigate the relationship between the subjective evaluation value and the physiological indices.According to the flow of the physiological response shown in Figure 1, changes in both the arousal level and thermal comfort affect the physiological response.To find indices (out of the 329 physiological parameters) that corresponded to the arousal level, we used data from sections when the arousal level changed, but the value of the thermal comfort sensation was maintained constantly.On the other hand, to find indices that corresponded to thermal comfort, we used data from sections when the value of the thermal comfort sensation changed and the arousal level was maintained constantly.

Relationship between the Drowsiness Level and Physiological Indices
As a result of the correlation analysis, the correlation coefficients between values of the drowsiness level vote and physiological indices were calculated using data from 57 sets in which the value of the drowsiness level vote varied, but the value of the comfort sensation vote remained at 0 (comfortable).There was a significant correlation between the drowsiness level and 102 physiological indices (data N = 57, r > 0.339, p < 0.01).The indices in relation to the ECGs were not included in these parameters; however, the indices in relation to the EEGs were included.Thus, this result showed that the relationship between the reaction of the central nervous system and changes in arousal levels is more significant than that between the reaction of the autonomic nervous system and changes in arousal levels.

Relationship between Thermal Comfort and Physiological Indices
As a result of the correlation analysis, the correlation coefficients between the values of the comfort sensation vote and physiological indices were calculated using the data from 98 sets in which the value of the comfort sensation vote varied, but the value of the drowsiness level vote was between 0 (alert) and 1 (slightly drowsy).There was a significant correlation between the value of the comfort sensation vote and seven physiological indices in relation to skin temperature, EEGs, and ECGs (data N = 98, r > 0.259, p < 0.01).The change in the mean skin temperature is related to thermal stimulation from the outer environment, which is transmitted to the central nervous system and perceived as thermal comfort, thus affecting the parameters of the EEGs.It was observed that changes in the thermal comfort affected the autonomic nervous system, which in turn affected the indices in relation to the ECGs.It appears that this process affected the result of the correlation analysis in this experiment.

Calculation of the Evaluation Index Using Multiple Regression Analysis
To propose an index that evaluates the changes in each arousal level and thermal comfort states, multiple regression analysis was performed using the physiological indices that have a significant correlation with the value of subjective evaluation.It was found that 102 parameters had a significant correlation with the drowsiness level vote, and seven parameters with the comfort sensation vote.These were thus considered as explanatory variables.Because an explanatory variable should have a high correlation with the subjective sensation vote, to obtain a high multiple regression coefficient, we selected an explanatory variable for use in the multiple regression analysis using the following process.

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Indices that had a significant correlation with the value of subjective evaluation were sorted according to their correlation coefficient from high to low (x1, x2, . . .x102).

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x1 was selected as the explanatory variable, since it had the highest correlation coefficient with the value of subjective evaluation.
• x2 was selected as the explanatory variable if there was no significant correlation between x2 and x1.

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xn was selected as the explanatory variable if there was no significant correlation between xn and all the parameters selected previously as explanatory variables.
(a) Evaluation Index indicating the Arousal Level The result of the multiple regression analysis 1 (Yd) is shown in Table 1, and the regression equation obtained from the result is expressed in Equation ( 2).The variables of the regression equation include the indices of the EEGs.As a result, the coefficient of determination, R where Xd 1 , Xd 2 , Xd 3 , Xd 4 , and Xd 5 are the high alpha content of T7, beta per alpha content of F7, beta content of F3, low beta content of F7, and alpha content of Fp1, respectively.We defined Yd as the index of the arousal level.To confirm the possibility that Yd can evaluate the arousal level even when the thermal comfort changed, we calculated Yd from the data obtained from five subjects for whom the arousal level and thermal comfort changed considerably, and then performed a correlation analysis between Yd and the evaluation value of the drowsiness level.As a result of the correlation analysis, the correlation coefficient (R) was calculated to be 0.726, and there was a significant correlation between Yd and the evaluation value of the drowsiness level.Therefore, this result suggests that Yd can be used to evaluate the drowsiness level, even when there are changes in the thermal comfort.

(b) Evaluation Index Indicating Thermal Comfort
The result of the multiple regression analysis 2 (Yc) is shown in Table 2, and the regression equation obtained from the result is expressed in Equation (3).The variable for the regression equation included the indices of the skin temperature and EEGs.As a result, the coefficient of determination, R 2 , in relation to Yc was 0.528.Yc = −23.372+ 0.697 Xc 1 + 0.172 Xc 2 + 0.142 Xc 3 (3) where Xc 1 , Xc 2 , and Xc 3 are the MST (Mean Skin Temperature), alpha per high-beta content of C4, and alpha per high beta content of P4, respectively.We defined Yc as the index of thermal comfort.To confirm the possibility that Yc can evaluate thermal comfort even when there were changes in the drowsiness level, we calculated Yc from the data of nine subjects, excluding the data of participant D whose thermal comfort vote and drowsiness level vote changed slightly.We thus confirmed the relationship between Yc and the value of thermal comfort.As a result, the correlation coefficient (R) was calculated to be 0.728, and there was a significant correlation between Yc and the value of thermal comfort (p < 0.01).Therefore, this result suggests that Yc can be used to evaluate thermal comfort even when there are changes in the drowsiness level.
Table 1 shows the result of the multiple regression 1 (Yd), and Table 2 shows the result of the multiple regression 2 (Yc).Figure 9 shows the relationship between the drowsiness level vote and Yd(a), and the relationship between the comfort sensation and Yc (b)   (c) Review of Continuous Evaluation using Yd and Yc As mentioned in Section 2.2., indices that can continuously evaluate the drowsiness level and thermal comfort of occupants are needed to clarify the design requirements related to thermal factors that can improve these states of the occupants.Therefore, we conducted a time-series analysis with Yd and Yc to confirm the possibility that Yd and Yc can continuously evaluate arousal levels and thermal comfort.We calculated the time series of Yd using data from subject C, who seemed to be drowsy frequently throughout the experiment, and compared these data with those of the drowsiness level, which was recorded at intervals of 10 s by analyzing the facial expressions of the subject based on Zilberg's method [20].The time series analyses of the normalized Yd and the drowsiness level of subject C are shown in Figures 10(a  (c) Review of Continuous Evaluation using Yd and Yc As mentioned in Section 2.2., indices that can continuously evaluate the drowsiness level and thermal comfort of occupants are needed to clarify the design requirements related to thermal factors that can improve these states of the occupants.Therefore, we conducted a time-series analysis with Yd and Yc to confirm the possibility that Yd and Yc can continuously evaluate arousal levels and thermal comfort.We calculated the time series of Yd using data from subject C, who seemed to be drowsy frequently throughout the experiment, and compared these data with those of the drowsiness level, which was recorded at intervals of 10 s by analyzing the facial expressions of the subject based on Zilberg's method [20].The time series analyses of the normalized Yd and the drowsiness level of subject C are shown in Figure 10a,b.The figures clearly show the trend of changes in both indicators.It can be seen that Yd and values of the drowsiness level show a similar trend.For example, the red circles in Figure 10a,b indicate that values of the drowsiness level and the normalized Yd are higher than 3 (significantly drowsy) and 0, respectively, at the same time.Furthermore, it can be seen from Figure 11 that there is a similar trend between Yc and the value of the comfort sensation vote.In Figure 11a,b, the value of the comfort sensation drops to −3 (very uncomfortable) at 30 min, and at the same time, the value of Yc drops and becomes a negative number.Thereafter, the value of the comfort sensation vote remains at an uncomfortable state (−3: Very uncomfortable, −2: Uncomfortable), and similarly, the value of Yc remains at a negative value.These results suggest that Yd and Yc can be used to evaluate the drowsiness level and thermal comfort of occupants continuously.indicators.It can be seen that Yd and values of the drowsiness level show a similar trend.For example, the red circles in Figures 10(a) and 10(b) indicate that values of the drowsiness level and the normalized Yd are higher than 3 (significantly drowsy) and 0, respectively, at the same time.Furthermore, it can be seen from Figure 11 that there is a similar trend between Yc and the value of the comfort sensation vote.In Figures 11(a) and 11(b), the value of the comfort sensation drops to −3 (very uncomfortable) at 30 min, and at the same time, the value of Yc drops and becomes a negative number.Thereafter, the value of the comfort sensation vote remains at an uncomfortable state (−3: Very uncomfortable, −2: Uncomfortable), and similarly, the value of Yc remains at a negative value.These results suggest that Yd and Yc can be used to evaluate the drowsiness level and thermal comfort of occupants continuously.Figure 10   Furthermore, it can be seen from Figure 11 that there is a similar trend between Yc and the value of the comfort sensation vote.In Figures 11(a) and 11(b), the value of the comfort sensation drops to −3 (very uncomfortable) at 30 min, and at the same time, the value of Yc drops and becomes a negative number.Thereafter, the value of the comfort sensation vote remains at an uncomfortable state (−3: Very uncomfortable, −2: Uncomfortable), and similarly, the value of Yc remains at a negative value.These results suggest that Yd and Yc can be used to evaluate the drowsiness level and thermal comfort of occupants continuously.

(d) Review of the Utility and Validity of the Evaluation Index
To review the utility and validity of the evaluation index, as an example, we set the threshold value in relation to Yd and the drowsiness level.When the threshold value of Yd is sets as 2.3, as shown in Figure 12, Yd can be used as the classifier, which can classify the drowsiness level between level 0, 1, 2, or more with an 86.7% accuracy.This result suggests that there is a significant difference in the physiological reaction between the drowsiness level of 1 (slightly drowsy) and 2 (moderately drowsy), and is a similar trend to the results of a previous study about the relation between the arousal levels and physiological indices of a driver [21], though the task of the subjects is different from that of the previous study (arithmetic task vs driving).This result also suggests that Yd is a valid indicator of the drowsiness level of occupants to classify between the drowsiness level of 1 (slightly drowsy) and 2 (moderately drowsy).Figure 12 shows the setting of the threshold value in relation to Yd.To review the utility and validity of the evaluation index, as an example, we set the threshold value in relation to Yd and the drowsiness level.When the threshold value of Yd is sets as 2.3, as shown in Figure 12, Yd can be used as the classifier, which can classify the drowsiness level between level 0, 1, 2, or more with an 86.7% accuracy.This result suggests that there is a significant difference in the physiological reaction between the drowsiness level of 1 (slightly drowsy) and 2 (moderately drowsy), and is a similar trend to the results of a previous study about the relation between the arousal levels and physiological indices of a driver [21], though the task of the subjects is different from that of the previous study (arithmetic task vs driving).This result also suggests that Yd is a valid indicator of the drowsiness level of occupants to classify between the drowsiness level of 1 (slightly drowsy) and 2 (moderately drowsy).Figure 12 shows the setting of the threshold value in relation to Yd.

Conclusions
In this study, we aimed to design a thermal environment that can improve the indicators of both the arousal level and feelings of thermal comfort.We hypothesized that thermal stimulation due to cooling can improve the arousal level of occupants, after which thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation.To verify the hypothesis, we measured physiological indices, values of subjective evaluation, and performances of arithmetic tasks throughout several thermal conditions in which the indoor ambient temperature was changed.In addition, we investigated the relationships between them to identify the indices that can be used to evaluate the arousal levels and thermal comfort of occupants.As a result, the following findings were noted:  When the indoor ambient temperature decreased and then increased, both the arousal level and thermal comfort of occupants remained at high levels.This result suggests that the hypothesis of this study was verified and changes in the indoor ambient temperature can be used to improve both thermal comfort and the arousal level of occupants.


We proposed the evaluation indices of thermal comfort and the drowsiness level of occupants.
It was observed that the drowsiness level and thermal comfort of occupants can be evaluated quantitatively and continuously using Yd and Yc, which were obtained from the equation consisting of physiological indices in relation to EEGs, ECGs, and skin temperature.

Conclusions
In this study, we aimed to design a thermal environment that can improve the indicators of both the arousal level and feelings of thermal comfort.We hypothesized that thermal stimulation due to cooling can improve the arousal level of occupants, after which thermal comfort can be improved while maintaining high arousal levels due to the removal of thermal stimulation.To verify the hypothesis, we measured physiological indices, values of subjective evaluation, and performances of arithmetic tasks throughout several thermal conditions in which the indoor ambient temperature was changed.In addition, we investigated the relationships between them to identify the indices that can be used to evaluate the arousal levels and thermal comfort of occupants.As a result, the following findings were noted:

•
When the indoor ambient temperature decreased and then increased, both the arousal level and thermal comfort of occupants remained at high levels.This result suggests that the hypothesis of this study was verified and changes in the indoor ambient temperature can be used to improve both thermal comfort and the arousal level of occupants.

•
We proposed the evaluation indices of thermal comfort and the drowsiness level of occupants.It was observed that the drowsiness level and thermal comfort of occupants can be evaluated quantitatively and continuously using Yd and Yc, which were obtained from the equation consisting of physiological indices in relation to EEGs, ECGs, and skin temperature.

Figure 1 .
Figure 1.Flow of physiological responses with the thermal stimulus.

Figure 1 .
Figure 1.Flow of physiological responses with the thermal stimulus.


Condition A: The indoor ambient temperature was maintained at 27 °C. Condition B: The indoor ambient temperature was decreased from 27 °C to 20 °C, and then increased from 20 °C to 27 °C. Condition C: The indoor ambient temperature was decreased from 27 °C to 20 °C, and then maintained at 20 °C.

Figure 2 .
Figure 2. Experimental conditions and environmental procedure.

Figure 2 .
Figure 2. Experimental conditions and environmental procedure.

15 Figure 3 .
Figure 3. Field photos of the experimental scene.

Figure 3 .
Figure 3. Field photos of the experimental scene.

4 .
Results and Discussion 4.1.Characteristics of the Arousal Level and Thermal Comfort Corresponding to Changes in the Indoor Ambient Temperature 4.1.1

Figure 4 .
Figure 4. Results of the value of the thermal comfort sensation vote.

Figure 5 .
Figure 5. Results of the value of the drowsiness level sensation vote.

Figure 4 .
Figure 4. Results of the value of the thermal comfort sensation vote.

Figure 4 .
Figure 4. Results of the value of the thermal comfort sensation vote.

Figure 5 .
Figure 5. Results of the value of the drowsiness level sensation vote.

Figure 5 .
Figure 5. Results of the value of the drowsiness level sensation vote.

Figure 8 .
Figure 8. Result of response time.

Figure 8 .
Figure 8. Result of response time.

Figure 8 .
Figure 8. Result of response time.

Figure 9 .
Figure 9.The relationship between the drowsiness level and Yd (a), the comfort sensation and Yc (b).
) and 10(b).The figures clearly show the trend of changes in both

Figure 9 .
Figure 9.The relationship between the drowsiness level and Yd (a), the comfort sensation and Yc (b).
Figures 10 and 11 show, respectively, changes in the normalized Yd (a) and drowsiness level (b), (subject C) and changes in Yc (a) and the comfort sensation vote (b) (subject B).

Figure 10 .
Figure 10.Changes in the normalized Yd (a) and drowsiness level (b), (subject C).(a) Time series of normalized Yd, (b) Time series of the value of the drowsiness level.

Figure 10 .
Figure 10.Changes in the normalized Yd (a) and drowsiness level (b), (subject C): (a) Time series of normalized Yd, (b) Time series of the value of the drowsiness level.

Figure 10 and
Figure 11 show, respectively, changes in the normalized Yd (a) and drowsiness level (b), (subject C) and changes in Yc (a) and the comfort sensation vote (b) (subject B).

Figure 10 .Figure 11 .
Figure 10.Changes in the normalized Yd (a) and drowsiness level (b), (subject C).(a) Time series of normalized Yd, (b) Time series of the value of the drowsiness level.

15 Figure 11 .
Figure 11.Changes in Yc (a) and the value of the comfort sensation vote (b): Time series of the value of the comfort sensation vote, (subject B).

Figure 12 .
Figure 12.Setting of the threshold value in relation to Yd.

Figure 12 .
Figure 12.Setting of the threshold value in relation to Yd.