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Appl. Sci. 2019, 9(4), 622; https://doi.org/10.3390/app9040622

Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine

1
Department of Chemistry, National Taiwan Normal University, Taipei 116, Taiwan
2
Graduate Institute of Applied Mechanics, National Taiwan University, Taipei 106, Taiwan
3
Advanced Research Center for Green Materials Science and Technology, National Taiwan University, Taipei 106, Taiwan
*
Authors to whom correspondence should be addressed.
Received: 17 January 2019 / Revised: 31 January 2019 / Accepted: 6 February 2019 / Published: 13 February 2019
(This article belongs to the Special Issue Applied Nanosensors for Food Toxicants and Environmental Pollutants)
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Abstract

Tyrosinase, chitosan, and reduced graphene oxide (rGO) are sequentially used to modify a screen-printed carbon electrode (SPCE) for the detection of dopamine (DA), without interference from uric acid (UA) or ascorbic acid (AA). The use of tyrosinase significantly improves the detection’s specificity. Cyclic voltammetry (CV) measurements demonstrate the high sensitivity and selectivity of the proposed electrochemical sensors, with detection limits of 22 nM and broad linear ranges of 0.4–8 μM and 40–500 μM. The fabricated tyrosinase/chitosan/rGO/SPCE electrodes achieve satisfactory results when applied to human urine samples, thereby demonstrating their feasibility for analyzing DA in physiological samples. View Full-Text
Keywords: screen printing; electrochemical biosensor; tyrosinase; chitosan; dopamine; reduced graphene oxide screen printing; electrochemical biosensor; tyrosinase; chitosan; dopamine; reduced graphene oxide
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Liu, C.-Y.; Chou, Y.-C.; Tsai, J.-H.; Huang, T.-M.; Chen, J.-Z.; Yeh, Y.-C. Tyrosinase/Chitosan/Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for Sensitive and Interference-Free Detection of Dopamine. Appl. Sci. 2019, 9, 622.

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