Evaluation of Some Rhodes Grass (Chloris gayana) Genotypes for Their Salt Tolerance, Biomass Yield and Nutrient Composition

: The livestock productivity in Ethiopia is seriously constrained by the shortage of fodder due to increasing soil salinization. Therefore, restoration of salt-affected lands into productive soils through salt-tolerant forages and improved irrigation and crop management practices is crucial for enhancing the productivity of the livestock sector in Ethiopia. In this three-year study, pot trials were conducted to evaluate the impact of ﬁve different soil salinity levels (i.e., 0, 5, 10, 15, and 20 dS m − 1 ) on plant growth, biomass production, and nutrient quality attributes of three Rhodes grass ( Chloris gayana ) genotypes (ILRI-6633, ILRI-7384, CV-massaba). Increasing soil salinity negatively affected germination percentage (GP) and mean germination time (MGT) of all genotypes. For all salinity levels, the highest GP was observed for ILRI-6633 and the lowest for CV-massaba. Plant height and chlorophyll content for ILRI-6633 was higher than the other two genotypes. The crude protein (CP) content was higher in low dry matter-producing genotype (ILRI-7384). The performance of ILRI-6633 at all salinity levels was superior to the other two genotypes. CV-massaba genotype performed better under low to medium soil salinity conditions. Therefore, ILRI-6633 and CV-massaba genotypes have excellent potential to increase forage production in salt-affected areas of Ethiopia.


Introduction
Saline soils are now becoming a growing threat to sustainable crop production in the irrigated lands of the semi-arid areas of the world [1,2]. Currently, over 1000 million ha (Mha) are inflicted with the different levels of salinity problems [3], and an additional 0.25-0.5 Mha of agricultural land is lost annually [4]. The salinity development is mainly caused by the presence of soluble salts in the water used for irrigation, hot and dry weather conditions, and excessive use of poor quality groundwater for irrigation [5,6]. Salinity problems are wide-spread in the Asian and African regions. In Ethiopia, approximately 11 Mha of land (i.e., 9% of the country's total landmass and 13% of the irrigated area) is exposed to salinity, of which 8 Mha have combined salinity and sodicity problems whereas the remaining 3 Mha are only sodic in nature [7,8].
In Ethiopia, saline soils are more dominant in the Awash Valley and semi-arid lowlands where 9% of the total population lives [8]. The growing prevalence of salt-affected soils is undermining the sustainability of irrigated agriculture in Ethiopia, as it reduces natural biodiversity as well as farm and livestock productivity. The situation is expected to aggravate in the future due to factors induced by climate change. The ongoing land degradation has grave implications for future food security and economic development of Ethiopia. With an annual growth rate of 2.48%, securing food and

Study Area
The field trials were conducted for three years (2016-2018) at the Werer Agricultural Research Center (WARC), Amibara district of the Afar region of Ethiopia. WARC is located at an altitude of 740 m above mean sea level between 9 • 12 8 N latitude and 40 • 15 21 E longitude ( Figure 1). The study area is mainly characterized as semi-arid with mean annual temperatures ranging between 19 • C and 34 • C. The mean annual Class A pan evaporation is 2800 mm, which is five times higher than the mean annual rainfall (570 mm) [17]. The study area consists of an extensive alluvial plain, which was historically formed by the deposits of the Awash River. The area has flat topography with a slope gradient ranging between 1-2%. The predominant soil types are weak-structured Vertisols and Fluvisols. The Vertisols are silty clay to clay whereas Fluvisols are sandy loam to silty loam in texture [18]. The constituents of Fluvisols are muscovite/illite clay minerals while Vertisols are dominated by montmorillonite clay minerals. Appl. Sci. 2019, 9, x FOR PEER REVIEW 3 of 12

Experimental Design and Salinity Treatments
The pot trials were conducted under a controlled environment. NaCl weighing 7.3, 14.6, 21.4 and 29.2 g was mixed with 6 kg soil packed in each pot to accomplish soil salinity levels of 5, 10, 15 and 20 dS m −1 , respectively. The control pot was established without any addition of NaCl. Two Chloris gayana genotypes (ILRI-6633; ILRI-7384) obtained from the International Livestock Research Institute (ILRI) and one local genotype from WARC (CV-massaba) were used for this study. Farmers in Ethiopia widely use these genotypes without much knowledge about their quality and quantity parameters. Treatments were factorially combined and arranged in a completely randomized design with three replications. Irrigations were applied according to crop evapotranspiration (ETc = ETo × Kc) values plus 10% for leaching requirements using good quality canal water (EC = 0.2 dS m −1 ). The reference evapotranspiration (ETo) was calculated with the modified Penman-Monteith equation (FAO-56) using climatic data collected from the field trial site. The crop coefficient (Kc) values were taken from the FAO-56 publication.

Data Collection and Analysis
Five seeds of each genotype were planted in each pot. Seeds that produced full radicle were considered as germinated. The data regarding mean germination time, germination percentage, number of tillers per plant, and plant height were collected from all pots. Chloris gayana grasses were sustained for up to 75 days after planting. At the time of harvesting, the shoot and root dry matter weight was recorded. The first germination count was done after five days of plantation. Because a saline environment often causes a delay in seed germination, the counting of the seedlings was done on the 10th and 15th day after plantation. The Germination Percentage (GP) was calculated according to Ashraf et al. (2005) [19]. (1) Mean Germination Time (MGT) was calculated using the following equation [20].
where n = number of germinated seeds on day D, and D = number of days from the start of germination.
Field trial site

Experimental Design and Salinity Treatments
The pot trials were conducted under a controlled environment. NaCl weighing 7.3, 14.6, 21.4 and 29.2 g was mixed with 6 kg soil packed in each pot to accomplish soil salinity levels of 5, 10, 15 and 20 dS m −1 , respectively. The control pot was established without any addition of NaCl. Two Chloris gayana genotypes (ILRI-6633; ILRI-7384) obtained from the International Livestock Research Institute (ILRI) and one local genotype from WARC (CV-massaba) were used for this study. Farmers in Ethiopia widely use these genotypes without much knowledge about their quality and quantity parameters. Treatments were factorially combined and arranged in a completely randomized design with three replications. Irrigations were applied according to crop evapotranspiration (ETc = ETo × Kc) values plus 10% for leaching requirements using good quality canal water (EC = 0.2 dS m −1 ). The reference evapotranspiration (ETo) was calculated with the modified Penman-Monteith equation (FAO-56) using climatic data collected from the field trial site. The crop coefficient (Kc) values were taken from the FAO-56 publication.

Data Collection and Analysis
Five seeds of each genotype were planted in each pot. Seeds that produced full radicle were considered as germinated. The data regarding mean germination time, germination percentage, number of tillers per plant, and plant height were collected from all pots. Chloris gayana grasses were sustained for up to 75 days after planting. At the time of harvesting, the shoot and root dry matter weight was recorded. The first germination count was done after five days of plantation. Because a saline environment often causes a delay in seed germination, the counting of the seedlings was done on the 10th and 15th day after plantation. The Germination Percentage (GP) was calculated according to Ashraf et al. (2005) [19].

GP =
Total germinated seeds Total number o f seeds Mean Germination Time (MGT) was calculated using the following equation [20].
where n = number of germinated seeds on day D, and D = number of days from the start of germination. The plant samples were dried and ground for analyzing crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and ash content (AC) using standard procedures [21,22]. Chlorophyll (SPAD units) content was also measured. The data were analyzed using analysis of variance (ANOVA) technique [23] for factorial CRD using SAS 9.3 software (SAS Institute, Cary, NC, USA). The least significant test was used to decipher the main and interaction effects of treatments at 5% level of significance (P < 0.05).  (Figure 2a). The highest GP at each salinity level was obtained for ILRI-6633, followed by ILRI-7384 except for higher salinity level of 20 dS m −1 where CV-massaba performed better than ILRI-7384. dS m −1 where CV-massaba performed better than ILRI-7384.

Results and Discussion
The germination of all three genotypes was delayed with the increasing salinity, i.e., from eight days in control (0 dS m −1 ) to more than 12 days for higher salinity (20 dS m −1 ) (Figure 2b). The local genotype CV-massaba germinated faster than the other two genotypes under all salinity levels. This shows that CV-massaba is more tolerant to salinity as far as germination is concerned. On average, the delay in MGT was 4, 13.3, 21.7, and 39.1% at 5, 10, 15, and 20 dS m −1 , respectively. The average MGT for all genotypes was 7.2 days in control, with ILRI-6633 being the maximum (7.9 days) and CV-massaba the minimum (6.8 days). At the highest salinity (20 dS m −1 ), the average MGT for ILRI-7384 was 12.2 days, followed by 12.1 days for ILRI-6633 and 11.2 days for CV-massaba. This shows that although CV-massaba germinated faster, its germination percentage was lower than the other two genotypes. In conclusion, the GP and MGT of all genotypes was negatively influenced by increasing salt stress in the soil. These results agree with the findings of Horst et al. (1989) [24] for perennial ryegrass and Iqbal et al. (2006) [25] for the teff grass.
Crop genotypes that can effectively germinate under salt stress conditions do not necessarily qualify for a good seedling growth [26]. However, in our case, ILRI-6633 and CV-massaba genotypes showed promising results not only in terms of germination percentage (GP) and mean germination time (MGT) but also seedling growth. Studies have shown that the crops with higher germination percentage have the capacity to establish themselves effectively on moderate to high saline soils (ECe = 5-12 dS m −1 ) [17]. Although it is difficult to generalize pot results for field conditions, ILRI-6633 and CV-massaba genotypes can potentially germinate and establish in moderate to high saline areas. This will help in producing high biomass because yield losses due to poor germination and inadequate crop stand establishment will be minimum [27][28][29].

Plant Height, Number of Tillers and Chlorophyll SPAD Value
The plant growth is affected by salinity because of a number of factors, including osmotic stress, ionic toxicity, lack of minerals, and physiological and biochemical responses [30]. This study reveals that at the seedling stage, there was a significant reduction in plant height, number of tillers per plant, and plant fresh and dry matter weight of three Chloris gayana genotypes with the increasing salinity levels. However, high chlorophyll content (SPAD value) was detected in salt-tolerant genotypes. These higher chlorophyll values can be ascribed to an improved photosynthetic rate and higher dry The germination of all three genotypes was delayed with the increasing salinity, i.e., from eight days in control (0 dS m −1 ) to more than 12 days for higher salinity (20 dS m −1 ) (Figure 2b). The local genotype CV-massaba germinated faster than the other two genotypes under all salinity levels. This shows that CV-massaba is more tolerant to salinity as far as germination is concerned. On average, the delay in MGT was 4, 13.3, 21.7, and 39.1% at 5, 10, 15, and 20 dS m −1 , respectively. The average MGT for all genotypes was 7.2 days in control, with ILRI-6633 being the maximum (7.9 days) and CV-massaba the minimum (6.8 days). At the highest salinity (20 dS m −1 ), the average MGT for ILRI-7384 was 12.2 days, followed by 12.1 days for ILRI-6633 and 11.2 days for CV-massaba. This shows that although CV-massaba germinated faster, its germination percentage was lower than the other two genotypes.
In conclusion, the GP and MGT of all genotypes was negatively influenced by increasing salt stress in the soil. These results agree with the findings of Horst et al. (1989) [24] for perennial ryegrass and Iqbal et al. (2006) [25] for the teff grass.
Crop genotypes that can effectively germinate under salt stress conditions do not necessarily qualify for a good seedling growth [26]. However, in our case, ILRI-6633 and CV-massaba genotypes showed promising results not only in terms of germination percentage (GP) and mean germination time (MGT) but also seedling growth. Studies have shown that the crops with higher germination percentage have the capacity to establish themselves effectively on moderate to high saline soils (ECe = 5-12 dS m −1 ) [17]. Although it is difficult to generalize pot results for field conditions, ILRI-6633 and CV-massaba genotypes can potentially germinate and establish in moderate to high saline areas. This will help in producing high biomass because yield losses due to poor germination and inadequate crop stand establishment will be minimum [27][28][29].

Plant Height, Number of Tillers and Chlorophyll SPAD Value
The plant growth is affected by salinity because of a number of factors, including osmotic stress, ionic toxicity, lack of minerals, and physiological and biochemical responses [30]. This study reveals that at the seedling stage, there was a significant reduction in plant height, number of tillers per plant, and plant fresh and dry matter weight of three Chloris gayana genotypes with the increasing salinity levels. However, high chlorophyll content (SPAD value) was detected in salt-tolerant genotypes. These higher chlorophyll values can be ascribed to an improved photosynthetic rate and higher dry matter production [31,32]. The reduction in chlorophyll content under marginal environments has been observed in many previous studies [19,25,31].
The plant height and the total number of tillers of three Chloris gayana genotypes under different soil salinity conditions is given in Table 1  The number of tillers per plant is one of the most important agronomic traits because it determines the biomass yield of the forage grasses. The results indicate that higher salinity conditions significantly affect the tiller production at maturity. The average number of tillers per plant was found to be 7.2, 7.1, 6.6, 5.9, and 4.8 at 0, 5, 10, 15, and 20 dS m −1 , respectively (Table 1). At 0 dS m −1 , ILRI-7384 gave maximum number of tillers per plant (9.4) followed by CV-massaba (6.9), and ILRI-6633 (5.4). A similar pattern was observed at higher salinity conditions (20 dS m −1 ) where the maximum and the minimum number of tillers per plant were recorded in ILRI-7384 (5.8) and ILRI-6633 (3.1), respectively. These results coincide with the earlier findings which show that morphological and physiological traits of plants such as the plant height, number of tillers per plant, and fresh and dry biomass yields tend to decrease with the increasing levels of salinity [31,33,34]. Figure 3 shows the chlorophyll content (SPAD value) of three Chloris gayana genotypes. The SPAD values were found to be consistent up to a salinity level of 10 dSbm −1 . However, a significant reduction in SPAD values at salinity levels of 15 dSbm −1 and 20 dSbm −1 was observed. For all salinity levels, highest SPAD values were found in ILRI-6633 followed by ILRI-7384 and CV-massaba. The highest SPAD value (63.6) was recorded in ILRI-6633 at moderate salinity level (10 dSbm −1 ). However, for 20 dSbm −1 , SPAD values decreased significantly for all three genotypes except for ILRI-6633. Similar trends of chlorophyll content reductions with the increasing salinity have also been observed for different crops [31,34].
Appl. Sci. 2019, 9, x FOR PEER REVIEW 6 of 12 The number of tillers per plant is one of the most important agronomic traits because it determines the biomass yield of the forage grasses. The results indicate that higher salinity conditions significantly affect the tiller production at maturity. The average number of tillers per plant was found to be 7.2, 7.1, 6.6, 5.9, and 4.8 at 0, 5, 10, 15, and 20 dS m −1 , respectively (Table 1). At 0 dS m −1 , ILRI-7384 gave maximum number of tillers per plant (9.4) followed by CV-massaba (6.9), and ILRI-6633 (5.4). A similar pattern was observed at higher salinity conditions (20 dS m −1 ) where the maximum and the minimum number of tillers per plant were recorded in ILRI-7384 (5.8) and ILRI-6633 (3.1), respectively. These results coincide with the earlier findings which show that morphological and physiological traits of plants such as the plant height, number of tillers per plant, and fresh and dry biomass yields tend to decrease with the increasing levels of salinity [31,33,34]. Figure 3 shows the chlorophyll content (SPAD value) of three Chloris gayana genotypes. The SPAD values were found to be consistent up to a salinity level of 10 dSbm −1 . However, a significant reduction in SPAD values at salinity levels of 15 dSbm −1 and 20 dSbm −1 was observed. For all salinity levels, highest SPAD values were found in ILRI-6633 followed by ILRI-7384 and CV-massaba. The highest SPAD value (63.6) was recorded in ILRI-6633 at moderate salinity level (10 dSbm −1 ). However, for 20 dSbm −1 , SPAD values decreased significantly for all three genotypes except for ILRI-6633. Similar trends of chlorophyll content reductions with the increasing salinity have also been observed for different crops [31,34].   Figure 4 shows the impact of different salinity levels on shoot and root dry matter of three Chloris gayana genotypes. The highest shoot dry matter (71.2 g/pot) was obtained in ILRI-6633 genotype whereas the lowest (60.3 g/pot) was recorded in ILRI-7384. The reduction in shoot dry matter was more pronounced at salinity levels of 15 dSbm −1 and 20 dSbm −1 . The overall reduction in shoot dry matter from 0 to 20 dSbm −1 was found to be 18.0 g/pot for ILRI-6633, 21.5 g/pot for ILRI-7384, and 27.5 g/pot for CV-massaba. This demonstrates that ILRI-6633 genotype has the lowest reduction in shoot dry matter with the increasing salinity.

Root and Shoot Dry Matter
Appl. Sci. 2019, 9, x FOR PEER REVIEW 7 of 12 whereas the lowest (60.3 g/pot) was recorded in ILRI-7384. The reduction in shoot dry matter was more pronounced at salinity levels of 15 dSbm −1 and 20 dSbm −1 . The overall reduction in shoot dry matter from 0 to 20 dSbm −1 was found to be 18.0 g/pot for ILRI-6633, 21.5 g/pot for ILRI-7384, and 27.5 g/pot for CV-massaba. This demonstrates that ILRI-6633 genotype has the lowest reduction in shoot dry matter with the increasing salinity.   (Figure 4b). For all salinity levels, the highest root dry matter was achieved for ILRI-6633 followed by CV-massaba. The maximum root dry matter for ILRI-6633 and CV-massaba was obtained at low to medium salinity levels (5-10 dS m −1 ). This suggests that the performance of ILRI-6633 with regard to root dry matter was superior to the other two genotypes. The average reduction in root dry matter ranged from 11.3-16.3 g/pot with an increase in salinity from 5-20 dS m −1 . The declining trends in the root dry matter with increasing salinity levels can be ascribed by the fact that in saline soils, plants spend more energy to get water and nutrients from the soil. This situation negatively affects the yield and the quality of the plant [3,[35][36][37][38].

Nutrient Composition
Forage grasses are considered to be the most remunerative form of animal feed in semi-arid regions. The nourishing value of forages mainly depends on their nutritional composition such as   (Figure 4b). For all salinity levels, the highest root dry matter was achieved for ILRI-6633 followed by CV-massaba. The maximum root dry matter for ILRI-6633 and CV-massaba was obtained at low to medium salinity levels (5-10 dS m −1 ). This suggests that the performance of ILRI-6633 with regard to root dry matter was superior to the other two genotypes. The average reduction in root dry matter ranged from 11.3-16.3 g/pot with an increase in salinity from 5-20 dS m −1 . The declining trends in the root dry matter with increasing salinity levels can be ascribed by the fact that in saline soils, Appl. Sci. 2019, 9, 143 8 of 12 plants spend more energy to get water and nutrients from the soil. This situation negatively affects the yield and the quality of the plant [3,[35][36][37][38].

Nutrient Composition
Forage grasses are considered to be the most remunerative form of animal feed in semi-arid regions. The nourishing value of forages mainly depends on their nutritional composition such as crude protein (CP) and fiber and ash contents [2]. Crude protein is an important element of the animal diet that enhances their milk-producing capacity and maintains meat quality [16]. Both CP and ash contents of three Chloris gayana genotypes exhibited a decreasing trend with increasing soil salinity. The highest CP (6.1%) and ash (15.5%) contents were obtained in ILRI-6633 and ILRI-7384 genotypes at low salinity level (5 dS m −1 ), whereas CV-massaba reported the lowest crude protein (3.8%) and ash content (12.4) at the same salinity level ( Figure 5). However, CV-massaba genotype produced a higher CP content at salinity levels of 10-15 dS m −1 . Generally, low dry matter-producing genotypes are known to have higher CP content whereas high dry matter producing accessions are low in nutrition at low salinity levels [30,39]. All three genotypes produced high CP and ash content at low to medium salinity (0-10 dS m −1 ) levels while a falling trend was observed at higher salinity levels (15-20 dS m −1 ). This shows that these varieties are more suitable for low to moderate salinity conditions. Appl. Sci. 2019, 9, x FOR PEER REVIEW 8 of 12 crude protein (CP) and fiber and ash contents [2]. Crude protein is an important element of the animal diet that enhances their milk-producing capacity and maintains meat quality [16]. Both CP and ash contents of three Chloris gayana genotypes exhibited a decreasing trend with increasing soil salinity. The highest CP (6.1%) and ash (15.5%) contents were obtained in ILRI-6633 and ILRI-7384 genotypes at low salinity level (5 dS m −1 ), whereas CV-massaba reported the lowest crude protein (3.8%) and ash content (12.4) at the same salinity level ( Figure 5). However, CV-massaba genotype produced a higher CP content at salinity levels of 10-15 dS m −1 . Generally, low dry matter-producing genotypes are known to have higher CP content whereas high dry matter producing accessions are low in nutrition at low salinity levels [30,39]. All three genotypes produced high CP and ash content at low to medium salinity (0-10 dS m −1 ) levels while a falling trend was observed at higher salinity levels (15-20 dS m −1 ). This shows that these varieties are more suitable for low to moderate salinity conditions. Neutral detergent fiber (NDF) mainly consists of hemicellulose, cellulose, and lignin. From the feeding point of view, lower NDF values are desirable for fodder as well as grains [40,41]. The NDF values for all three genotypes showed an increasing trend with the growing salt stress. Differences in NDF among the Chloris gayana genotypes show a similar trend as of CP. ILRI-6633 reported the highest NDF (70.9-73.9%) value whereas the lowest NDF values (67.5-72.9%) were observed in CVmassaba for all salinity levels ( Figure 6). The average Invitro dry matter digestibility content (IvDMDC) was 42.9% for control, 41% at 5 dS m −1 , 41.6% at 10 dS m −1 , 40.2% at 15 dS m −1 , and 36.1% Neutral detergent fiber (NDF) mainly consists of hemicellulose, cellulose, and lignin. From the feeding point of view, lower NDF values are desirable for fodder as well as grains [40,41]. The NDF values for all three genotypes showed an increasing trend with the growing salt stress. Differences in NDF among the Chloris gayana genotypes show a similar trend as of CP. ILRI-6633 reported the highest NDF (70.9-73.9%) value whereas the lowest NDF values (67.5-72.9%) were observed in CV-massaba for all salinity levels ( Figure 6). The average Invitro dry matter digestibility content (IvDMDC) was 42.9% for control, 41% at 5 dS m −1 , 41.6% at 10 dS m −1 , 40.2% at 15 dS m −1 , and 36.1% at 20 dS m −1 (Figure 6). The highest IvDMDC was obtained in ILRI-6633 at control while the lowest was observed in CV-massaba at 20 dS m −1 .
Appl. Sci. 2019, 9, x FOR PEER REVIEW 9 of 12 at 20 dS m −1 (Figure 6). The highest IvDMDC was obtained in ILRI-6633 at control while the lowest was observed in CV-massaba at 20 dS m −1 .

Conclusions and Recommendations
The effects of five soil salinity levels on the agronomic and nutritional quality parameters of three Chloris gayana genotypes (ILRI-6633, ILRI-7384, and CV-massaba) for dry and hot conditions of Ethiopia were evaluated. The agronomic parameters such as the germination rate, germination time, plant height, and shoot and root dry matter weights showed a decreasing trend with the increasing soil salinity levels for all Chloris gayana genotypes. The highest germination rate, lowest germination time, maximum plant height, and the number of tillers per plant were observed in ILRI-6633 compared to ILRI-7384 and CV-massaba genotypes. For all three genotypes, the root length was more severely affected than the shoot length at all salinity levels. The increasing soil salinity negatively affected the shoot and root dry matter of three Chloris gayana genotypes. The reduction in shoot dry matter was more noticeable at the higher salinity levels (i.e., 15-20 dS m −1 ).
The chlorophyll content values were consistent up to 10 dS m −1 , whereas significant reduction was observed at higher salinity levels (15-20 dS m −1 ). The highest crude protein (CP) and ash content (AC) values were obtained for ILRI-6633 and ILRI-7384 genotypes at 5 dS m −1 , whereas CV-massaba reported lowest CP value (3.8%) and ash content (12.4) at the same salinity level.

Conclusions and Recommendations
The effects of five soil salinity levels on the agronomic and nutritional quality parameters of three Chloris gayana genotypes (ILRI-6633, ILRI-7384, and CV-massaba) for dry and hot conditions of Ethiopia were evaluated. The agronomic parameters such as the germination rate, germination time, plant height, and shoot and root dry matter weights showed a decreasing trend with the increasing soil salinity levels for all Chloris gayana genotypes. The highest germination rate, lowest germination time, maximum plant height, and the number of tillers per plant were observed in ILRI-6633 compared to ILRI-7384 and CV-massaba genotypes. For all three genotypes, the root length was more severely affected than the shoot length at all salinity levels. The increasing soil salinity negatively affected the shoot and root dry matter of three Chloris gayana genotypes. The reduction in shoot dry matter was more noticeable at the higher salinity levels (i.e., 15-20 dS m −1 ).
The chlorophyll content values were consistent up to 10 dS m −1 , whereas significant reduction was observed at higher salinity levels (15-20 dS m −1 ). The highest crude protein (CP) and ash content (AC) values were obtained for ILRI-6633 and ILRI-7384 genotypes at 5 dS m −1 , whereas CV-massaba reported lowest CP value (3.8%) and ash content (12.4) at the same salinity level.
From the feeding point of view, lower values of neutral detergent fiber (NDF) are desirable. NDF values showed an increasing trend with the growing salt stress. The genotypes with lower CP values tend to have higher NDF values. On average, the maximum NDF values were found in ILRI-6633 and the lowest in CV-massaba genotype for all salinity levels. The average in vitro dry matter digestibility content (IvDMDC) also showed a decreasing trend with the increasing salinity levels. This means Chloris gayana forage grass has higher digestibility.
In conclusion, ILRI-6633 is identified as the most salt-tolerant, high yielding and better nutritive forage grass than the other two genotypes. CV-massaba is superior to the other two genotypes in terms of germination under saline conditions. Therefore, these two genotypes can potentially be grown to increase farm productivity in salt-affected areas of Ethiopia.