A Review Paper on “Graphene Field Emission for Electron Microscopy”

: Although good ﬁeld emission from graphene has been demonstrated from a wide variety of different microfabricated structures, very few of them can be used to improve the design of cold ﬁeld emitters for electron microscopy applications. Most of them consist of densely packed nano-emitters, which produce a large array of defocused overlapping electron beams, and therefore cannot be subsequently focused down to a single nanometer electron probe. This paper reviews the kind of single-tip cathode structures suitable in cold ﬁeld emission guns for instruments such as scanning electron microscopy, transmission electron microscope or scanning transmission electron microscopy, and reviews progress in fabricating them from graphene-based materials.


Introduction
The Dyke group proposed a variety of applications of a cold field emission (CFE) source and formed the first company to produce CFE based commercial products in the early 1960s [1]. The successful application of CFE cathodes to electron microscopy, including both scanning electron microscopy (SEM) and scanning transmission electron microscopy (STEM), was achieved by a group led by Crewe et al. in the late 1960s [2,3]. For over 40 years, state-of-the-art metal tip CFE sources used for commercial high-resolution STEM consist of a single crystal tungsten sharpened wire, etched in a specific plane (such as the <310> plane) to generate the lowest work function, and its tip radius is in the 100-200 nm range. However, they suffer from many well-known practical challenges, such as an inherent ultrahigh vacuum (UHV) condition requirement (<10 −9 Torr) and relatively large current instabilities (~40% fall-off within the first hour), which have impeded their wide-ranging use [4]. As a result, most of the latest generation electron microscopes use the more stable and reliable Schottky electron source, which has a lower brightness (~10 8 Am −2 sr −1 V −1 ) and higher energy spread (~0.5 eV) [5][6][7][8]. Although CFE sources have the desirable characteristics of a low energy spread (~0.3 eV) and high brightness (~10 9 Am −2 sr −1 V −1 ), their lifetimes need to be improved (>1 year) and their current stabilities need to be reduced (<1%) [4,5,9].
Nanostructures in the form of nanotubes [10][11][12][13], nanotips [14], and nanowires [15,16] have the potential to be used as cathodes in CFE sources. Their large field enhancement factors stem from nanometer-scale emission sites which can, in some cases, produce electron beams with brightness values, one order of magnitude higher than the state-of-the-art single crystal tungsten cathode tips. Despite their promising electron optics performance, they have not been successfully integrated into any commercial electron microscopes, and this is largely due to the same technological difficulties faced by single crystal tungsten cathode-tips (stringent vacuum, low lifetime and high beam current fluctuations). However, in addition, they require the delicate transfer/mounting process of an individual emitter being attached to the tip of a sharpened supporting metal wire, and this is very difficult to do without incurring significant misalignment or damage.
Since the discovery of two-dimensional (2D) graphene in 2004, graphene has attracted much attention as a potential candidate for CFE source emitters, due to its high aspect ratio (the lateral size to the thickness) [17][18][19] and excellent thermal, mechanical, and electrical properties [20][21][22][23]. Excellent field emission from graphene has been demonstrated from a wide variety of different microfabricated structures, and most of them consist of densely packed nano-emitters that produce a large array of defocused overlapping electron beams, and therefore cannot be subsequently defocused down to a single nanometer electron probe [24][25][26], making them unsuitable for electron microscopy/lithography applications. This paper primarily focuses on reviewing developments in the field emission of graphene-based emitters for electron microscopy/lithography applications. In the first part of the paper, it will summarize basic field emission theory in relation to single crystal W field emitters (CFE) oriented in the <310> or <111> directions and other well-developed one-dimensional (1D) nanostructure CFE electron emitters. In the second part, different types of graphene field emitters will be introduced, including graphene film emitters, graphene point emitters and graphene ring emitters. The main theme is primarily on how graphene can be used to make single-tip cathodes in electron sources suitable for electron microscopy/lithography applications, whose electron beams can be focused down to a single nanometer electron probe. Finally, a summary and outlook are given towards the future development of high brightness-high resolution CFE electron gun on graphene-based nanomaterials.

Theory of Cold Field Electron Emission
Electron sources are essential tools for investigation into a broad range of field emission devices [27][28][29][30][31]. For electron microscopy applications, electron sources can be divided into two main categories, known as thermionic and field emission [32]. A thermionic electron gun uses Joule-heating induced thermal excitation to allow electrons to overcome the energy barrier between the metal tip and the vacuum. The electron beam that it produces is inherently incoherent, having a source size in the tens of microns range, a relatively large energy spread (2 eV) and poor brightness (~10 6 Am −2 sr −1 V −1 ). A CFE electron gun overcomes these limitations by decreasing the virtual source size to about 3 nm (by significantly decreasing the emission tip to about 100 nm radius) and the energy spread to about 0.3 eV (operating the source at room temperature), in which electrons tunnel from a solid surface to the vacuum under a high local electric field [1]. The mechanism of the electron emission process is based on the Fermi-Dirac distribution for a free electron gas in the potential energy barrier between the metal tip and the vacuum [33].
Fowler and Nordheim first found a classical theory describing the behavior of field emission currents from a metallic surface in 1928 [34]. In the theory, the relationship between the emission current (I) and the tip field strength (F) is expressed as, where the constant A has the dimension of area m 2 , φ is the work function in eV, and F is the local electric field on the cathode tip, given by F = βV ext /d, with β being the field enhancement factor, V ext the external applied voltage, and d the anode to cathode tip spacing. The cathode tip is geometrically sharp, and the local electric field (F) is intensified by a factor of β on the tip surface. To achieve cold field electron emission, F is typically around few volts per nanometer. A plot of ln I/V 2 ext against 1/V ext will have a slope of m = − 6.44 × 10 9 φ 1.5 d/β , the well-known Fowler-Nordheim (FN) plot.

Source Electron Optics Parameters
For successful electron microscopy applications, a high source brightness (B r ) and a low energy spread (∆E) are always needed to provide a high-resolution focused electron beam. The calculation of B r requires knowledge of the virtual source size (d v ), which is given by the following expression [35]: where I is the angular intensity, obtained by dividing the emission current over the acceptance solid angle subtending by the source. Bronsgeest et al. derived an analytical formula to calculate the virtual source size [35], given by the following expression: where r tip is the tip radius and < E t > is an initial average tangential energy defined as < E t > = ehF/ √ (8mφ), with F the local electric field strength, φ the work function, m α the angular magnification andh the reduced Planck constant. In these formulas, m α and F can be estimated from direct ray tracing simulations and when used together with an experimentally measured angular intensity profile, the source brightness B r can be estimated. For high brightness electron beam applications, statistical Coulomb interactions lead to radial broadening of the virtual source size due to lateral electron-electron interactions, which in turn, lower the brightness [36]. The calculation of statistical Coulomb interactions within an electron source is not straightforward, since the electron potential and beam size vary in the gun region. A semi-analytical technique known as the slice method was introduced to do this, as reported in a recent paper by Cook et al. [37]. First, the electric potential distribution in the gun region is solved numerically, and then the beam size in the region is determined by the simulated direct ray tracing of electron trajectories that leave the cathode tip. The gun region is subsequently divided into small segments over which the voltage and beam size is assumed to remain constant, and an analytical expression is used to estimate the trajectory displacement effect due to the lateral electron-electron interactions.
It should be noted that the method just outlined avoids the need to measure the virtual source diameter d v experimentally, and this is because direct measurements of the virtual source diameter are not trivial. One way to achieve it is to put the source in a transmission electron microscope as the emitter and obtain a magnified source image [7]. A different method to measure d v is to use a de-magnifying lens to focus the electron beam from the gun unit into a spot which is source-size dominated (relatively low contributions from lens aberrations), and then measure the spot size of the beam by scanning it across a knife edge [38,39]. Another method is to place a knife edge at a small distance (less than a few micrometers) from an emitter and to measure the angular width of the Fresnel fringes patterns [13,40]. In this technique, the intensity distribution shape of the source is assumed to be a Gaussian function, and the values of magnification and electron wavelength are needed to extract d v .
Another important electron gun parameter, apart from brightness, is its energy spread. The energy spread, through the chromatic aberration of the objective lens, degrades the final spatial resolution of an electron optical system, particularly at high relative beam energy spreads. Ideally, the energy distribution (∆E) of the emitted electron beam should be as narrow as possible while maintaining sufficient brightness. From the theoretical point of view, the total energy distribution (TED) of electron emission in the thermal field regime was first derived by Young based on the free-electron model as [41]: where kT = 0.155 eV at room temperature, J FN is the well-known FN emission current density and d is the tunneling parameter given by: d = 9.76 × 10 −11 F/φ 1/2 t(y). The variable t(y) is a slowly-varying function of y = 3.79 × 10 −5 F 1/2 /φ and can be approximated by the formula t(y) = 1 + 0.1107 y 1. 33 . The analytical formula is valid provided kT/d < 0.7 and y < 1. The longitudinal electron-electron interactions (known as Boersch effect) will add to the intrinsic source energy spread which causes an additional enlargement of the energy spread [36]. According to Knauer's model of a spherical electric field around an emitter of tip radius r tip , the energy broadening (in eV) due to Coulomb interactions is given by [42]:

The State-Of-The-Art Electron Source Used in Electron Microscopes
The state-of-the-art electron sources used for commercial high-resolution electron microscopes (such as Nion UltraSTEM 100 manufactured in the Nion company, Kirkland, WA, USA [43] and JEOL ARM200F Aberration-Corrected S/TEM manufactured in the JEOL USA, Inc., Peabody, MA, USA [44]) are made from a single crystal W field emitter (CFE) oriented in the <310> direction with a work function of 4.5 eV [4]. Electron emission from the cathode occurs when the field strength at the cathode tip typically exceeds 4 V/nm, allowing electrons to escape from its surface by quantum tunnelling [4]. As electrons are accelerated through the gun unit, the wide-angle ones are filtered out by the first and second anode plate holes, and they exit the gun unit in the form of a rotationally symmetric electron beam which appears emanate from a single point on the axis (virtual source). The relatively small virtual source size of cold field emission guns (a few nanometers in diameter) is one of the main reasons for them having the highest brightness of all electron sources used in focused electron beam instruments. Another advantage is that they have a relatively low energy spread (0.2-0.3 eV), and it is these types of properties that make cold field emission sources the best type of electron source for forming nano-sized electron probes with high current intensity. Figure 1A,B show typical SEM images of the W<310> and W<111> CFE sources, respectively. As observed in the SEM images, the emitters have a spherical apex with a radius of around 130 nm at the end of the cathode-tip. For a single crystal W field emitter, the emitter surface is required to be etched in a <310> or <111> plane at the emitter apex, due to their lower work function values, and this can be achieved by using a zone melting technique [45]. The source reduced brightness (B r ) versus the full width at half maximum (FWHM(E)) values for the W<310> and W<111> sources are plotted in Figure 1C. For the state-of-the-art W<310> field emitter, a reduced brightness value of 4 × 10 9 Am −2 sr −1 V −1 has been reported, and the measured energy spread ranges from 0.33 eV to 0.48 eV [4]. The relatively large values of B r mainly come from the small values of virtual source size d v (~3 nm) and the large values of angular current density (~100 µA/sr). Although the state-of-the-art single crystal W field emitters have desirable electron optical performances, they suffer from some well-known practical engineering problems. One difficulty comes from the presence of the oxide layer on the metallic tungsten surface and subsequently affects the stability of the electron emission, requiring the use of UHV levels in the gun unit (<10 −10 Torr). It has been shown the oxide layer has a significant impact on the tunneling current stability since the thin oxide layer acting as a n-type semiconductor with φ equal to 5.59-5.7 eV involves noise mechanisms typical for semiconductor devices [46]. Another difficulty comes from residual gas molecules around the emitting surface which ionize positively under the impact of high emission current. The ionized ions are then attracted back striking the emitting surface under the strong local electric field, causing large current fluctuations and reducing the lifetime. Thus, regular thermal heating of the tip is needed to reset the current fluctuations [15]. Moreover, the single crystal W field emitters are made very sharp in order for the local electric field strength to be strong enough for quantum tunneling. The relatively small tip size of the cathode tip makes it more vulnerable to instability and damage.

Nanoscale Point-Cathode Emitters
Nanostructures in the form of one-dimensional (1D) nanowires, nanotubes, and nanocones have attracted much attention in past decades and have been demonstrated for many potential applications. In particular, their use as electron field emitters exhibit excellent geometric, electrical and mechanical properties, as compared to conventional metallic cold field emitters [47][48][49][50]. However, there are also significant technical challenges that have yet to be resolved.
Generally, preparation of an individual nanoscopic CFE electron emitter is an extremely labor-intensive delicate process. To mount a single nanowire or nanotube onto a supporting tip, the most common method is to use microscopic manipulators under the observation of an electron microscope. Figure 2A,C show a single LaB 6 nanowire [16] and a single carbon nanotube (CNT) [10] mounted on a sharpened W tip, respectively. The ends of the emitter were deposited with platinum (or carbon) by electron beam induced deposition (EBID) in order to make good electrical contact and the final emitter structure is fitted into an electron gun unit as shown in Figure 2B, and its inset. Figure 2D,E show the mounting procedure for a carbon cone nanotip (CCnT) [14], where a CCnT was welded onto the apex of the supporting tip by using a gas injecting system. The whole process has a very high probability of incurring misalignment and damage.
These nanoscale point-cathode emitters also have the limitation that they need to be operated in an UHV level, in the 10 −11 to 10 −10 Torr range. For a LaB 6 point-cathode emitter with a diameter of nearly 60 nm, a reduced brightness (B r ) value of 2.77 × 10 11 Am −2 sr −1 V −1 and an energy spread value of 0.367 eV have been reported in a vacuum with a base pressure of 10 −10 Torr. The short-term stability profile of the LaB 6 point-cathode is 0.32% over 60 s, and the emission current deviates from the mean within 5% throughout a week-long test. For the CNT and CCnT point-cathode emitters with diameters of around 10 nm, the source electron optics parameters were reported to be 8.2 × 10 9 Am −2 sr −1 V −1 , 0.48 eV, and 1.6 × 10 9 Am −2 sr −1 V −1 , 0.32 eV respectively. The vacuum pressure requirements are even more stringent, typically around 10 −11 Torr. It was found that there exists some unrecoverable structure damage to the CNT during electron emission under the high vacuum (HV) conditions of 9 × 10 −8 Torr, and this leads to emission reduction or failure [51]. In addition to its inherent UHV requirements, the CNT emitter is reported to have relatively large fluctuations in emission current, e.g., up to 24% for an emission current of about 2 µA over 6 hours with a sampling rate of 3 × 10 −3 Hz.
These nanoscale CFE electron sources have not as yet been successfully integrated in any commercial electron microscopes due to the aforementioned significant technical challenges, and studies of nanowire, nanotube, and nanocone based cold field emitters have remained at the research level. Meanwhile, other developments in CFE electron emitter research have come from the use of materials such as graphene.

Graphene-Based Cold Field Emitters
Fast development of two-dimensional (2D) graphene in recent years has led to a variety of different applications for field emission based devices [20,53]. Compared with other metallic or 1D nanoscale CFE electron sources, graphene is well known for possessing several desirable properties: (1) it has excellent thermal, mechanical, and electrical characteristics [17,21]; (2) its work function can be significantly lowered both by direct contact with metals (effectively doping it), and through the application of intense electric fields [54,55]; and (3) it is extremely flexible. Graphene CFE electron sources fall into two main categories: the single-tip cathode field nano-emitter (which includes point cathodes and ring cathodes) and graphene film cathodes, which consist of densely packed graphene nano-emitters. Only the former can be used for electron microscopy applications, as the latter produces a large array of defocused overlapping electron virtual sources that cannot be subsequently focused down to a single nano-sized probe.

Graphene Film Emitters
To achieve successful electron emission from graphene, it is crucial to orientate thin graphene edges or control the dimension of graphene protrusions on some supporting substrate, in order to sufficiently enhance the applied electric field to produce quantum tunneling. Until now, many emerging technologies have become available for fabricating vertically aligned graphene nanosheets (VG) or graphene protrusions. The plasma-enhanced chemical vapor deposition (PECVD) is the most widely used way to synthesize large-scale VG graphene films [56], which offers the advantages of a lower substrate temperature and higher growth selectivity ( Figure 3A). Koh et al. presented a pulsed laser deposition (PLD) method [57] to directly fabricate graphene thin film onto metal nano-sized Spindt tips by using the transformation of solid carbon deposited from a pulsed laser system at low temperature. The main drawback of these two deposition methods is the difficulty of controlling the graphene film ordering and density without incurring problems such as the screening effect [58,59]. Wu et al. applied the electrophoretic deposition (EPD) technique [60] to fabricate a large area of homogeneous single-layer graphene films, and this processing technique is more economic and versatile compared to PECVD ( Figure 3B). Screen printing technology is a sophisticated technology widely used in large-scale applications coating on various substrates. A screen-printed graphene cathode was fabricated by Qian et al. [61] using high-yield graphene prepared by a modified Hummers method and a hydrazine hydrate reduction process ( Figure 3C). Another efficient way to produce uniform nanometer-scale graphene protrusions is through transferring graphene onto some sharp supporting substrates ( Figure 3D) [24,25,62]. However, the exfoliated graphene by using either screen printing technologies, or electrophoretic deposition, or simple transfer have been found to lead to many defects to the as-grown graphene, that degrades its physical properties [63][64][65]. For graphene film emitters, high current densities, around 23 mAcm −2 , have been reported with good stability over a period of several hours [60]. Current density here, is calculated by dividing the total measured emission current by the average area on the substrate occupied by emitter sites. Although not suitable for electron microscopy applications, graphene film emitters are very useful for many non-focused electron beam applications, such as flat panel display [28,66], X-ray sources [67][68][69], etc.

Graphene Point-Cathode Field Emitters
Until now, graphene point-cathode field emitters for electron microscopy applications have been rarely studied due to the difficulties inherent in controlling graphene morphology. Tsai et al. [70] reported an idea of overlaying an ultra-thin (~1 nm) graphene flake on to a blunted tungsten probe by van der Waals forces, as shown in Figure 4A, which would make it applicable to single-column electron beam microscopy applications. However, this loosely hanging ultra-thin freestanding graphene flake is not suitable for practical applications, since the graphene can easily be damaged or detached from its supporting probe. More importantly, the emitting surface is not circular, it spans a few micrometers along one direction, and this means that it cannot subsequently be focused down to a single nanometer electron spot. For most single-tip point field emitters, the emission current is surface sensitive and the adsorption of any gas molecules will increase the surface work function [15,72]. A high emission current under excessive applied voltage ionizes many gas molecules around the emission surface and eventually triggers a vacuum arc. Therefore, the practical use of cold field emitters in electron microscopes typically requires cathode flashing at regular intervals to remove the residual contaminant molecules. Endo et al. [73] reported that CFE electron sources can be cleaned by in situ Joule heating during the emission process in HV conditions, which is well known as a "conditioning" process in many cold field emission experiments [71,74,75]. For example, during the first and second cycle of voltage ramping up from the graphene flake overlaid on the tungsten probe ( Figure 4A), the current-applied voltage characteristic presents a typical conditioning process, which is observed to deviate from the conventional FN plot. The third cycle of electron emission shows a well fitted FN plot with an emission current up to 6 µA. Similar work has been done by other researchers studying the field emission characteristics from reduced graphene oxide ( Figure 4B) and carbon nanowalls, and they have obtained high emission current, around several tens of microamperes [71,76].
Recently, the research group at the National University of Singapore, led by A. Khursheed, have presented the development of a completely new type of cold field emission electron source ( Figure 5A), a Graphene-Ni point cathode for electron microscopy systems [77]. They present a way of overcoming the morphological problems associated with micro-fabricating single-tip graphene emitters, by coating it on to a sharpened wire metal wire tip. In doing so, they have overcome many of the practical difficulties associated with conventional metallic tungsten CFE sources, such as misalignment, stringent vacuum requirements, and current instabilities. They reported stable field emission from a point cold field emission source that can operate in High Vacuum (HV), as opposed to stringent UHV conditions, and uses relatively large cathode-tip sizes (microns), an advantage created by their very low electric field strength requirements (0.5-1.5 V/nm) and low work function (~1.1 eV).
These features enable it to provide stable and repeatable emission properties, something which is not usually associated with single point cathode cold field emission sources. The Graphene-Ni point cathode was fabricated by a two-step process, which consists firstly of an electrochemical etching process to obtain a sharp Ni tip, and secondly, a graphene coating step of chemical vapor deposition (CVD). The Graphene-Ni point cathode for cathode tip-radius of 400 nm was shown to have a high emission current of about 5 µA under a relatively low applied electric field strength (0.5-1.5 V/nm) on the tip apex. In their study, direct ray tracing simulations of electron trajectory paths leaving the emitter surface were carried out to estimate m α and F, from which the virtual source size d v and gun brightness B r were then calculated by using Equations (2) and (3). The source brightness values range from 7 × 10 7 Am −2 sr −1 V −1 to 2.51 × 10 9 Am −2 sr −1 V −1 for cathode tip radii in the range of 130 nm to 800 nm. It is well known that there is a trade-off between the source brightness value and the tip size. On the one hand, a larger tip size has a greater emission area, thereby having a better current stability and can operate in much less stringent vacuum conditions [78]. One the other hand, a larger tip size has a larger virtual source size and a lower source brightness value. This trade-off is applicable to all CFE sources, and most of the previous studies on CNT, LaB 6 , and CCnT tips made great efforts to maximize gun brightness at the price of having poor gun current stability and stringent vacuum requirements. Another stand out feature of the Graphene-Ni point cathodes is their low energy spread ( Figure 5B). The measured energy spread ranges from 0.246 eV to 0.420 eV for tip radii in the range of 260 nm to 500 nm. The Graphene-Ni point cathode as presented here appears to have overcome many of the practical engineering difficulties. A comparison of typical operating parameters and electron optical properties for the Graphene-Ni point cathode and the state-of-the-art electron sources is listed in Table 1.

Graphene Ring-Cathode Field Emitters
Khursheed first proposed the idea of using a ring-cathode emitter for combining high spatial resolution with high beam current for focused electron beam applications in 2013 [79,80]. Khursheed presented electron focused beam designs based upon using ring-cathodes together with off-axis aberration correction techniques. The first step towards a practical implementation of this idea is to develop a ring-cathode emitter. The proposal of a field emission ring-cathode gun unit has the advantage of intrinsically being a large area field emitter, and it is therefore expected to overcome the well-known disadvantage of conventional point-cathode field emitters having relatively low total current (compared to earlier larger cathode electron gun designs). The much larger emission area of a ring-cathode field emitter, compared to a point-cathode field emitter is also expected to provide it with greater current stability.
Nguyen et al. [81] reported a versatile and robust method based on graphene growth on Ni templates and thermo-assisted removal of poly(methyl methacrylate) (PMMA) for fabrication of macroscopic and freestanding tubular graphene (TG) architectures. The TG architectures have a diameter of 50 µm with a wall thickness in the range of 2.1-2.9 nm ( Figure 6A). The individual TG emitter can produce an extremely high emission current, up to 0.46 mA at a very low external applied electric field of 0.68 V µm −1 . This most likely comes from ultrathin edges on graphitic walls, which consist of a great number of field emission sites. Another important observation is that the TG CFE electron source has good current stability and the stability is maintainable for days under HV conditions (2.2 × 10 −8 Torr). However, one limitation of TG architectures fabricated by Nguyen et al. is that the edges are very jagged rather than being smooth, making it hard to be directly used as a CFE electron source for electron microscopy applications. Moreover, transfer and alignment of such a large diameter graphene tube is an extremely delicate process.
Shao et al. [82] reported an in-situ fabrication technique, eliminating any transfer and alignment process. They obtained a graphene ring emitter, which consists of a graphene tube grown in situ on a sharp nickel wire tip ( Figure 6B). The diameter of the tubular graphene is about 5 µm while the edge of the tube is approximately 2.7 nm. They have obtained high emission current of~30 µA at the relatively low applied field of~1.75 V/mm. The ring-cathode field emitter is a new type of field emitter source, and suitable electron ring-focused beam columns will first need to be designed for it. When that is achieved, it is expected to a wide variety of applications in subjects such as lithography, mass spectrometer, X-ray tube and accelerator physics.

Conclusions
High brightness, low energy spread and good current stability are critical for CFE electron sources for electron microscopy applications. Although considerable research has been carried out into developing single crystal tungsten and other 1D nanostructure-based field emitters, they have as yet, faced many practical technological difficulties, such as stringent UHV requirements, poor current stability and fast emission decay. Therefore, other ways to make CFE electron sources need to be investigated. With the fast development of 2D graphene in recent years, graphene has been proven to have ultra-high aspect ratios, good electric conductivity, chemical inertness and mechanical hardness, and these properties make graphene-based field emitters an attractive possible alternative. Three types of graphene-based field emitters have been highlighted in this review: graphene film emitters, graphene point/edge-cathode emitters, and graphene ring-cathode emitters. Most graphene field emitters proposed and studied so however, involve field emission from multiple emission sites, and are therefore not suitable for electron microscopy applications. This paper has highlighted some recent promising developments in the category of single-point and ring-shaped graphene field emitters for electron microscopy applications, however, the investigation of graphene field emitters is still in an early stage of development, and there is still much room for further improvement. More emission characterization tests need to be performed, ones that can measure the source virtual source size, transverse coherent length, emission under a variety of different vacuum conditions, and emitter lifetime need to be made. An electron gun unit that can accommodate promising graphene emitters, which has its optical axis well aligned to the central axis of the cathode still needs to be developed. Beyond that, electron guns based upon the new class of graphene-based field emitters need to be fitted on to electron microscopes, and their performance critically compared to convention systems, in terms of parameters such as image resolution and signal-to-noise ratio.