Thermoresponsive Behavior of Magnetic Nanoparticle Complexed pNIPAm-co-AAc Microgels

Characterization of responsive hydrogels and their enhancement with novel moieties have improved our understanding of functional materials. Hydrogels coupled with inorganic nanoparticles have been sought for novel types of responsive materials, but the efficient routes for the formation and the responsivity of complexed materials remain for further investigation. Here, we report that responsive poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) hydrogel microparticles (microgels) are tunable by varying composition of co-monomer and crosslinker as well as by their complexation with magnetic nanoparticles in aqueous dispersions. Our results show that the hydrodynamic diameter and thermoresponsivity of microgels are closely related with the composition of anionic co-monomer, AAc and crosslinker, N,N′-Methylenebisacrylamide (BIS). As a composition of hydrogels, the higher AAc increases the swelling size of the microgels and the volume phase transition temperature (VPTT), but the higher BIS decreases the size with no apparent effect on the VPTT. When the anionic microgels are complexed with amine-modified magnetic nanoparticles (aMNP) via electrostatic interaction, the microgels decrease in diameter at 25 ◦C and shift the volume phase transition temperature (VPTT) to a higher temperature. Hysteresis on the thermoresponsive behavior of microgels is also measured to validate the utility of aMNP-microgel complexation. These results suggest a simple, yet valuable route for development of advanced responsive microgels, which hints at the formation of soft nanomaterials enhanced by inorganic nanoparticles.


Introduction
Stimuli-responsive hydrogels, that undergo the volume phase transition in response to external stimuli, have great value in the development of biocompatible smart materials for use in biosensors [1][2][3][4][5], artificial tissues [6], and many other utilities [2,4,7].The responsivity can be carefully designed to cause the swelling/deswelling of the hydrogels and most common cases are temperature and pH responsive behaviors [8][9][10][11].To achieve such responsivity, poly(N-isopropylacrylamide) (pNIPAm) and acrylic acid (AAc) are widely used due to the lower critical solution temperature (LCST) and the pK a , respectively [12,13].Above the LCST, the polymer network is favorable to be collapsed by chain-chain interactions, while above the pK a the network tends to swell due to Coulombic repulsion among the deprotonated acid groups [14][15][16].Colloidal hydrogel particles (microgels) are commonly prepared by aqueous free-radical precipitation polymerization due to the low polydispersity and accurate size control [13,17].These microgels have been extensively investigated for their fast response rate and nanoscale structure in comparison to those of bulk hydrogels [18,19].The development of multi-responsive hydrogels often requires different kinds of monomers for copolymerization, and even post-modifications by conjugating interesting molecules to the hydrogels [20,21].Therefore, efficient methods for the formation of such advanced hydrogels are highly desired.
Nanoparticles including gold nanoparticles (AuNPs) and magnetic nanoparticles (MNPs) have been widely applied in the development of functional nanosystems for optical modulators [5,22], biosensors [23,24], imaging agents [25], and biological interfacing technology [26].This suggests that complexation of the nanoparticles with responsive hydrogels allows the development of multipotent materials in a wide range of applications [27][28][29][30][31][32].For instance, AuNPs and MNPs are coupled with hydrogels via DNA hybridization, which shows photothermal and magnetic-field responsive activities, respectively [27,28].In addition, copolymerization in which AuNPs and MNPs are used as a seed material has been used to yield hybrid nanoparticle-hydrogels in core-shell structure.Although this method allows for many interesting applications of the nanoparticle-hydrogels, it also requires a careful optimization for synthetic procedures [29][30][31][32].Thus, there is a need for simple, fast, yet useful hybridization methods for the nanoparticle-hydrogel complexation, while retaining the hydrogel's responsivity.In this study, we aim to demonstrate that the pNIPAm-co-AAc microgels are aptly complexed with MNP, and the hybridized MNP-microgels show the characteristic thermoresponsivity in a repeatable manner.Our finding is distinguished from previous studies that utilize copolymerization of MNP-hydrogels by its simplicity and rapidity, allowing responsive pNIPAm-co-AAc microgels to acquire additional merits of magnetic nanoparticles for magneto-induced applications.

Microgel Synthesis
All microgels were synthesized via aqueous free-radical precipitation polymerization, as previously described [13,34].The syntheses were carried out by fixing the total monomer concentration constant at 70 mM while varying the AAc mole percent of 5%, 10%, and 20% with 2% fixed BIS mole percent and the BIS mole percent of 0%, 2%, and 5% with 10% fixed AAc mole percent that also accordingly adjusted the NIPAm mole percent.For instance, mole percent of NIPAm is 90%, 88%, and 85%, for 0% BIS/10% AAc, 2% BIS/10% AAc, and 5% BIS/10% AAc microgels, respectively.Microgel synthesis was performed in a three-neck, 100 mL round-bottom flask.Fifty milliliters of a filtered (0.2 µm filter, Pall Gelman Metricel, New York, NY, USA), aqueous solution of NIPAm, BIS, and the surfactant SDS (28.8 mg, 1 mM final concentration) were added into the reactor.The solution was heated to ~70 • C while degassing with N 2 and stirring with magnetic bar for ~1 h.After 1 h, AAc was added to the flask, yielding the total monomer concentration of 70 mM.At 10 min after the addition of AAc, polymerization was initiated by injection of 1.0 mL of an APS solution (45.64 mg, 2 mM).The reaction was terminated after an additional 6 h of heating at ~70 • C and stirring while degassing by a constant flow of N 2 gas.Following synthesis, the microgels were purified by using dynamic dialysis system (100 kD, Spectrum, Waltham, MA, USA).

MNP Synthesis
For the current experiments, zinc-doped iron oxide magnetic nanoparticles (MNPs) were synthesized following the previous reports [33,35,36].In brief, Fe(acac) 3 (353 mg), FeCl 2 (40 mg) and ZnCl 2 (30 mg) were dissolved in octyl ether with organic surfactants (oleic acid and oleylamine).The mixture was placed in a 50 mL three-neck round-bottom flask and heated at 300 • C for 1 h.Then, the reactor was cooled down to room temperature.The products were isolated by centrifugation after adding ethanol and the centrifuged magnetic nanoparticles are re-dispersed in toluene.For the amine functionalization, the MNPs are first coated by silica shell via a standard water-in-oil microemulsion sol-gel approach and the silica surface is further functionalized by aminopropyltrimethoxysilane (APTMS) [36].The size and shape of amine-modified magnetic nanoparticles (aMNPs) were estimated by imaging using TEM (JEM-2100, JEOL, Tokyo, Japan) (Figure 1).Zeta potential value of aMNPs was measured (19 mV) and the hydrodynamic size by dynamic light scattering (DLS) is 21 nm with polydispersity index (PDI) of 0.1.

MNP Synthesis
For the current experiments, zinc-doped iron oxide magnetic nanoparticles (MNPs) were synthesized following the previous reports [33,35,36].In brief, Fe(acac)3 (353 mg), FeCl2 (40 mg) and ZnCl2 (30 mg) were dissolved in octyl ether with organic surfactants (oleic acid and oleylamine).The mixture was placed in a 50 mL three-neck round-bottom flask and heated at 300 °C for 1 h.Then, the reactor was cooled down to room temperature.The products were isolated by centrifugation after adding ethanol and the centrifuged magnetic nanoparticles are re-dispersed in toluene.For the amine functionalization, the MNPs are first coated by silica shell via a standard water-in-oil microemulsion sol-gel approach and the silica surface is further functionalized by aminopropyltrimethoxysilane (APTMS) [36].The size and shape of amine-modified magnetic nanoparticles (aMNPs) were estimated by imaging using TEM (JEM-2100, JEOL, Tokyo, Japan) (Figure 1).Zeta potential value of aMNPs was measured (19 mV) and the hydrodynamic size by dynamic light scattering (DLS) is 21 nm with polydispersity index (PDI) of 0.1.

Dynamic Light Scattering (DLS) Analysis
The microgel was titrated by adding aliquots of 0.6 µg of aMNP in solution to a dilute solution of microgels (10 µL of purified microgels diluted to 1 mL) at pH 6.5 (~0.001 ionic strength) contained in a plastic cuvette.Each microgel solution was prepared before each measurement.The radius and thermoresponsive behavior of the microgels following titration was measured using Dynamic Light Scattering instrument (DLS, Zetasizer Nano ZSP, Malvern Panalytical, Malvern, UK).The radius value was an average of 12 individual radius measurements using a 5 s integration time for each measurement.For volume phase transition temperature (VPTT) measurements, the microgels were either first titrated with a given amount of aMNP or not.The solution was heated at an interval of 1 °C and the microgel size was determined by DLS.At each temperature, 5 consecutive runs of a sample were performed where each run was composed of 12 individual radius measurements using a 5 s integration time for each measurement.The error bars were estimated by the 5 consecutive runs of the same sample.

Transmission Electron Microscopy (TEM) Analysis
The microgel was complexed with aMNP in solution.The image of aMNP-microgels were analyzed by using a transmission electron microscope (TEM, JEM-2100, JEOL, Japan).

Results and Discussions
The responsive hydrogel nanoparticles are prepared by aqueous free-radical precipitation copolymerization of N-isopropylacrylamide (NIPAm) and acrylic acid (AAc) with the use of the cross-linker, N,N′-Methylenebisacrylamide (BIS).Microgels are hydrogel particles that are in a range

Dynamic Light Scattering (DLS) Analysis
The microgel was titrated by adding aliquots of 0.6 µg of aMNP in solution to a dilute solution of microgels (10 µL of purified microgels diluted to 1 mL) at pH 6.5 (~0.001 ionic strength) contained in a plastic cuvette.Each microgel solution was prepared before each measurement.The radius and thermoresponsive behavior of the microgels following titration was measured using Dynamic Light Scattering instrument (DLS, Zetasizer Nano ZSP, Malvern Panalytical, Malvern, UK).The radius value was an average of 12 individual radius measurements using a 5 s integration time for each measurement.For volume phase transition temperature (VPTT) measurements, the microgels were either first titrated with a given amount of aMNP or not.The solution was heated at an interval of 1 • C and the microgel size was determined by DLS.At each temperature, 5 consecutive runs of a sample were performed where each run was composed of 12 individual radius measurements using a 5 s integration time for each measurement.The error bars were estimated by the 5 consecutive runs of the same sample.

Transmission Electron Microscopy (TEM) Analysis
The microgel was complexed with aMNP in solution.The image of aMNP-microgels were analyzed by using a transmission electron microscope (TEM, JEM-2100, JEOL, Tokyo, Japan).

Results and Discussions
The responsive hydrogel nanoparticles are prepared by aqueous free-radical precipitation copolymerization of N-isopropylacrylamide (NIPAm) and acrylic acid (AAc) with the use of the cross-linker, N,N -Methylenebisacrylamide (BIS).Microgels are hydrogel particles that are in a range of sub-micron size and with high water content in their polymeric networks.PNIPAm-co-AAc microgels are responsive to external stimuli such as temperature and pH, resulting in the decrease or increase of the hydrodynamic radius of microgels, as shown in Figure 2a,b.In a previous study, we report that pNIPAm-co-AAc microgels are complexed with poly(allylaminehydrochloride) (PAH) via ion pairing, which shows a decrease or increase of the microgel's hydrodynamic size mainly due to the crosslinking and charge shielding effects [37].Thus, we hypothesize that pNIPAm-co-AAc microgels are able to form the complex structure with amine-functionalized magnetic nanoparticles (aMNPs) in similar mechanisms, which provides an easy and practical method for preparation of hybrid MNP-microgels, as shown in Figure 2c,d, while retaining the thermoresponsivity.Since TEM imaging has been widely used to visualize magnetic nanoparticles, we utilize TEM analysis which displays the image of aMNP incorporated pNIPAm-co-AAc microgels, as shown in Figure 3.In addition, zeta potential and DLS values are measured to confirm the aMNP-microgel complexation, which shows the increase of the surface charge and the decrease of hydrodynamic diameter upon addition of cationic aMNP into anionic microgel solution, as shown in Figure 4.  of sub-micron size and with high water content in their polymeric networks.PNIPAm-co-AAc microgels are responsive to external stimuli such as temperature and pH, resulting in the decrease or increase of the hydrodynamic radius of microgels, as shown in Figure 2a,b.In a previous study, we report that pNIPAm-co-AAc microgels are complexed with poly(allylaminehydrochloride) (PAH) via ion pairing, which shows a decrease or increase of the microgel's hydrodynamic size mainly due to the crosslinking and charge shielding effects [37].Thus, we hypothesize that pNIPAm-co-AAc microgels are able to form the complex structure with amine-functionalized magnetic nanoparticles (aMNPs) in similar mechanisms, which provides an easy and practical method for preparation of hybrid MNP-microgels, as shown in Figure 2c,d, while retaining the thermoresponsivity.Since TEM imaging has been widely used to visualize magnetic nanoparticles, we utilize TEM analysis which displays the image of aMNP incorporated pNIPAm-co-AAc microgels, as shown in Figure 3.In addition, zeta potential and DLS values are measured to confirm the aMNP-microgel complexation, which shows the increase of the surface charge and the decrease of hydrodynamic diameter upon addition of cationic aMNP into anionic microgel solution, as shown in Figure 4.   To characterize how the pNIPAm-co-AAc microgels respond to aMNPs, we measure the hydrodynamic diameter (Dh) of the microgels upon the addition of aMNPs in aqueous solutions, as shown in Figure 5.The use of dynamic light scattering (DLS) allows the determination of the average Dh, which shows the decrease of each Dh for various microgels, regardless of the AAc and BIS content in the microgels.In the case of different AAc content, the microgel size is decreased with positive correlation upon the addition of aMNPs.For instance, the diameter of microgels decreases from 630, 470, and 410 nm to 250, 250, and 300 nm for 20%, 10%, and 5% of AAc, respectively, as shown in Figure 5a.It is reasonable to consider the fact that higher content of AAc yields the larger Dh of microgels mainly due to Coulombic swelling providing room for higher deswelling in which the microgel charge is compensated by positive charge of aMNPs.In contrast, the higher content of BIS shows the smaller Dh of the microgels as measured by 760, 470, and 430 nm, respectively, while the acrylic acid ratio is constant at 10%, as shown in Figure 5b.Their response to addition of aMNPs is inversely correlated with BIS content in the microgels.It is interesting to note that the microgels with 0% BIS dramatically change in the Dh (760 to 350 nm) after initial titration with aMNPs and then keep the size with no further response to aMNPs.This is partially explained by the fact that crosslinker free microgels are highly deformable by eternal stimuli [38], resulting in such abrupt change in the hydrodynamic diameter for 0% BIS microgels.The microgels with 2% and 5% BIS also decrease in the size upon addition of aMNPs and their reactivity is lower than that of 0% BIS microgels.To characterize how the pNIPAm-co-AAc microgels respond to aMNPs, we measure the hydrodynamic diameter (Dh) of the microgels upon the addition of aMNPs in aqueous solutions, as shown in Figure 5.The use of dynamic light scattering (DLS) allows the determination of the average Dh, which shows the decrease of each Dh for various microgels, regardless of the AAc and BIS content in the microgels.In the case of different AAc content, the microgel size is decreased with positive correlation upon the addition of aMNPs.For instance, the diameter of microgels decreases from 630, 470, and 410 nm to 250, 250, and 300 nm for 20%, 10%, and 5% of AAc, respectively, as shown in Figure 5a.It is reasonable to consider the fact that higher content of AAc yields the larger Dh of microgels mainly due to Coulombic swelling providing room for higher deswelling in which the microgel charge is compensated by positive charge of aMNPs.In contrast, the higher content of BIS shows the smaller Dh of the microgels as measured by 760, 470, and 430 nm, respectively, while the acrylic acid ratio is constant at 10%, as shown in Figure 5b.Their response to addition of aMNPs is inversely correlated with BIS content in the microgels.It is interesting to note that the microgels with 0% BIS dramatically change in the Dh (760 to 350 nm) after initial titration with aMNPs and then keep the size with no further response to aMNPs.This is partially explained by the fact that crosslinker free microgels are highly deformable by eternal stimuli [38], resulting in such abrupt change in the hydrodynamic diameter for 0% BIS microgels.The microgels with 2% and 5% BIS also decrease in the size upon addition of aMNPs and their reactivity is lower than that of 0% BIS microgels.To characterize how the pNIPAm-co-AAc microgels respond to aMNPs, we measure the hydrodynamic diameter (Dh) of the microgels upon the addition of aMNPs in aqueous solutions, as shown in Figure 5.The use of dynamic light scattering (DLS) allows the determination of the average Dh, which shows the decrease of each Dh for various microgels, regardless of the AAc and BIS content in the microgels.In the case of different AAc content, the microgel size is decreased with positive correlation upon the addition of aMNPs.For instance, the diameter of microgels decreases from 630, 470, and 410 nm to 250, 250, and 300 nm for 20%, 10%, and 5% of AAc, respectively, as shown in Figure 5a.It is reasonable to consider the fact that higher content of AAc yields the larger Dh of microgels mainly due to Coulombic swelling providing room for higher deswelling in which the microgel charge is compensated by positive charge of aMNPs.In contrast, the higher content of BIS shows the smaller Dh of the microgels as measured by 760, 470, and 430 nm, respectively, while the acrylic acid ratio is constant at 10%, as shown in Figure 5b.Their response to addition of aMNPs is inversely correlated with BIS content in the microgels.It is interesting to note that the microgels with 0% BIS dramatically change in the Dh (760 to 350 nm) after initial titration with aMNPs and then keep the size with no further response to aMNPs.This is partially explained by the fact that crosslinker free microgels are highly deformable by eternal stimuli [38], resulting in such abrupt change in the hydrodynamic diameter for 0% BIS microgels.The microgels with 2% and 5% BIS also decrease in the size upon addition of aMNPs and their reactivity is lower than that of 0% BIS microgels.Thermoresponsivity of the microgels is evaluated by measuring the Dh at 25 • C and 50 • C five times, repetitively.The microgels with various AAc content (5%, 10%, 20%) show the decreased Dh at 50 • C, the temperature above the LCST, and their size is recovered by cooling at 25 • C. Such thermoresponsivity is consistently observed for the remaining cycles for all microgels, as shown in Figure 6a, and the Dh changes increase with the AAc content of microgels.We then test the effect of aMNP complexation with microgels on their thermoresponsivity, which illustrates repeated Dh changes in the cycles of temperature, as shown in Figure 6b.It is worthwhile to note that the thermoresponsivity of aMNP-microgels remains for the all cycles, while their initial size is smaller than those of the uncomplexed microgels.This finding is informative when the stimuli-responsive microgels are tailored for future applications.For instance, to design more sensitive microgels for nanoparticle binding, the higher AAc composition would be beneficial.This also suggests that electrostatic complexation of microgels with aMNP is an efficient route to prepare thermoresponsive MNP-microgels for various applications.
Thermoresponsivity of the microgels is evaluated by measuring the Dh at 25 °C and 50 °C five times, repetitively.The microgels with various AAc content (5%, 10%, 20%) show the decreased Dh at 50 °C, the temperature above the LCST, and their size is recovered by cooling at 25 °C.Such thermoresponsivity is consistently observed for the remaining cycles for all microgels, as shown in Figure 6a, and the Dh changes increase with the AAc content of microgels.We then test the effect of aMNP complexation with microgels on their thermoresponsivity, which illustrates repeated Dh changes in the cycles of temperature, as shown in Figure 6b.It is worthwhile to note that the thermoresponsivity of aMNP-microgels remains for the all cycles, while their initial size is smaller than those of the uncomplexed microgels.This finding is informative when the stimuli-responsive microgels are tailored for future applications.For instance, to design more sensitive microgels for nanoparticle binding, the higher AAc composition would be beneficial.This also suggests that electrostatic complexation of microgels with aMNP is an efficient route to prepare thermoresponsive MNP-microgels for various applications.Similarly, thermoresponsivity of the microgels with various BIS content (0%, 2%, 5%) is evaluated in the same temperature cycle mode, as shown in Figure 6c.The higher BIS content in microgels, the smaller the Dh at 25 °C, resulting in the lesser change in the Dh at 50 °C.The effects of BIS on the microgel thermoresponsivity are mimicking those of AAc, but in an inversed manner.Such trends are also measured in the case of aMNP-microgels, as shown in Figure 6d.Again, this finding suggests that the microgels with higher responsivity to nanoparticles are prepared with the lower BIS content.Thus, we confirm that the nanoparticle-microgel complexation is suitable to form advanced nanomaterials with the characteristic thermoresponsivity in a repeatable manner.Similarly, thermoresponsivity of the microgels with various BIS content (0%, 2%, 5%) is evaluated in the same temperature cycle mode, as shown in Figure 6c.The higher BIS content in microgels, the smaller the Dh at 25 • C, resulting in the lesser change in the Dh at 50 • C. The effects of BIS on the microgel thermoresponsivity are mimicking those of AAc, but in an inversed manner.Such trends are also measured in the case of aMNP-microgels, as shown in Figure 6d.Again, this finding suggests that the microgels with higher responsivity to nanoparticles are prepared with the lower BIS content.Thus, we confirm that the nanoparticle-microgel complexation is suitable to form advanced nanomaterials with the characteristic thermoresponsivity in a repeatable manner.
To characterize the thermoresponsive behavior of microgels, we also measure the Dh of microgels at 1 • C intervals from 25 to 55 • C, which allows for the determination of the volume phase transition temperature (VPTT), as shown in Figure 7.Although the microgels with various AAc begin to undergo transition in their size at ~31 • C, the ending temperature of the phase transition differs by 10 • C. As a result, the VPTTs of microgels are increased to 36, 37, and 41 • C for 5%, 10%, and 20% of AAc, respectively, as shown in Figure 7a and Table 1.The complexation of aMNPs with microgels slightly alters their thermoresponsivity by increasing VPTT and beginning/ending transition temperature with 2~3 • C, respectively, with the exception of the 5% AAc, as shown in Figure 7b.It is interesting to note that the initial size of aMNP-microgels is smaller than that of microgels alone but their deswelling yields similar size to the microgels.The results imply that maximum deswelling of microgels is coupled to their composition in the synthesis, while post-modification of microgels by aMNP complexation changes their properties including the Dh in swollen state, VPTT, and degree of transition.
The VPTTs of microgels are also evaluated by changing BIS content of the microgels, as shown in Figure 7c,d and Table 1.In contrast to the above experiments, the effect of BIS on the microgel's VPTT is not apparent, while the Dh of the microgels at 25 • C is clearly different, as shown in Figure 7c.The results suggest that AAc as a comonomer enables the modulation of both the VPTT and swollen degree of microgels, but BIS as a crosslinker favors the change of the swollen degree of microgels rather than the VPTT.However, the complexation of aMNP to microgels differs the VPTT by BIS content, as shown in Figure 7d.For instance, the VPTT of aMNP-microgels are increased to 38, 40, and 43 • C for 0%, 2%, and 5% of BIS, respectively, while the VPTT of microgels are retained at ~37 • C. It could be explained by considering the fact that amine groups of aMNP contribute to the charge balance of microgels.It is also worthwhile to note that the observed VPTT value (38 • C) for 5% BIS microgels (10% AAc,) are comparable to previously reported values (36.4 • C) [39].Our results suggest that the higher the BIS content in microgels, the higher the VPTT of aMNP-microgels.
To characterize the thermoresponsive behavior of microgels, we also measure the Dh of microgels at 1 °C intervals from 25 to 55 °C, which allows for the determination of the volume phase transition temperature (VPTT), as shown in Figure 7.Although the microgels with various AAc begin to undergo transition in their size at ~31 °C, the ending temperature of the phase transition differs by 10 °C.As a result, the VPTTs of microgels are increased to 36, 37, and 41 °C for 5%, 10%, and 20% of AAc, respectively, as shown in Figure 7a and Table 1.The complexation of aMNPs with microgels slightly alters their thermoresponsivity by increasing VPTT and beginning/ending transition temperature with 2~3 °C, respectively, with the exception of the 5% AAc, as shown in Figure 7b.It is interesting to note that the initial size of aMNP-microgels is smaller than that of microgels alone but their deswelling yields similar size to the microgels.The results imply that maximum deswelling of microgels is coupled to their composition in the synthesis, while post-modification of microgels by aMNP complexation changes their properties including the Dh in swollen state, VPTT, and degree of transition.
The VPTTs of microgels are also evaluated by changing BIS content of the microgels, as shown in Figure 7c,d and Table 1.In contrast to the above experiments, the effect of BIS on the microgel's VPTT is not apparent, while the Dh of the microgels at 25 °C is clearly different, as shown in Figure 7c.The results suggest that AAc as a comonomer enables the modulation of both the VPTT and swollen degree of microgels, but BIS as a crosslinker favors the change of the swollen degree of microgels rather than the VPTT.However, the complexation of aMNP to microgels differs the VPTT by BIS content, as shown in Figure 7d.For instance, the VPTT of aMNP-microgels are increased to 38, 40, and 43 °C for 0%, 2%, and 5% of BIS, respectively, while the VPTT of microgels are retained at ~37 °C.It could be explained by considering the fact that amine groups of aMNP contribute to the charge balance of microgels.It is also worthwhile to note that the observed VPTT value (38 °C) for 5% BIS microgels (10% AAc,) are comparable to previously reported values (36.4 °C) [39].Our results suggest that the higher the BIS content in microgels, the higher the VPTT of aMNP-microgels.