Production of Chlorella vulgaris Biomass in Tubular Photobioreactors during Different Culture Conditions

: Biomass of microalgae and the components contained in their cells can be used for the production of heat, electricity, and biofuels. The aim of the presented study was to determine the optimal conditions that will be the most favorable for the production of large amounts of microalgae biomass intended for energy purposes. The study analyzed the effect of the type of lighting, the time of lighting culture, and the pH of the culture medium on the growth of Chlorella vulgaris biomass. The experiment was carried out in vertical tube photobioreactors in three photoperiods: 12/12, 18/6, and 24/0 h (light/dark). Two types of lighting were used in the work: high-pressure sodium light and light-emitting diode. The increase in biomass was determined by the gravimetric method, by the spectrophotometric method on the basis of chlorophyll a contained in the microalgae cells. The number of microalgae cells was also determined with the use of a hemocytometer. The optimal conditions for the production of biomass were recorded at a neutral pH, illuminating the cultures for 18 h a day. The obtained results were 546 ± 7.88 mg · L − 1 dry weight under sodium lighting and 543 ± 1.92 mg · L − 1 dry weight under light-emitting diode, with maximum biomass productivity of 27.08 ± 7.80 and 25.00 ± 5.1 mg · L − 1 · d − 1 , respectively. The maximum content of chlorophyll a in cells was determined in the 12/12 h cycle and pH 6 (136 ± 14.13 mg · m − 3 ) under light-emitting diode and 18/6 h, pH 7 (135 ± 6.17 mg · m − 3 ) under sodium light, with maximum productivity of 26.34 ± 2.01 mg · m − 3 · d − 1 (light-emitting diode) and 24.21 ± 8.89 mg · m − 3 · d − 1 (sodium light). The largest number of microalgae cells (2.1 × 10 6 ) was obtained at pH 7 and photoperiod of 18/6 h under sodium light, and 12/12 h under light-emitting diode. Based on the results, it can be concluded that the determination of the optimal parameters for the growth and development of microalgae determines the production of their biomass, and such research should be carried out before starting the large-scale production process. In quantifying the biomass during cultivation, it is advantageous to use direct measurement methods. weight, measuring the content of chlorophyll a, and counting the number of cells using a hemocytometer.


Introduction
Microalgae are among the most widespread organisms. According to Guiry [1], it is estimated that there are up to 1 million species of algae on Earth. We meet them in the entire biosphere, but above all in fresh, salt, and saline waters. A characteristic feature of algae is the presence of chlorophyll [2,3], which enables photosynthesis to be carried out in each individual cell [4]. The efficiency of this process and the related biomass production is much higher compared to land plants [5].
Microalgae biomass has been widely used in various industries: pharmaceutical, food, animal feed, water purification, and biofuel production [6][7][8]. Under suitable conditions, microalgae convert solar energy into chemical energy of compounds accumulated in their cells, including not only in carbohydrates and proteins but also in lipids, which are used in the production of biodiesel [9,10]. The biomass yield and the biochemical composition of microalgae depend on environmental and physiological factors such as culture temperature, pH, lighting intensity and type, availability of nutrients, and carbon dioxide [11].
Lighting directly influences the growth of microalgae through photosynthesis. Algae cells, like most organisms, require not only access to light but also the dark phase, where photochemical processes occur and both ATP and NADPH are formed in algae cells [12]. Research on determining the optimal photoperiod in microalgae cultivation indicates that the efficiency of algal biomass production increases with the lengthening of the light to dark ratio [12,13]. When selecting the optimal light, the quality of the spectrum and the light intensity must be taken into account. The quality of the spectrum is defined by the absorption spectrum of chlorophyll and other components present in algae cells, which include phycobilins and carotenoids. The energy consumption of algae cells depends on the chemical conditions of the constitutive pigments [14]. The assimilation pigments have two main bands of blue-green light absorption with a wavelength of 450-475 nm and red light, 630-675 nm [15]. Lighting should be evenly distributed throughout the reactor allowing the photons to reach the cells. Excessive light intensity can lead to photooxidation and photoinhibition, while low light levels will reduce the growth of microalgae [16].
The pH of a culture medium not only affects the composition and lifetime of microalgae cells but also determines the availability of nutrients, the proper course of the photosynthesis process, and the uptake of carbon dioxide by cells [17]. The acidic conditions adversely affect the absorption of nutrients and disrupt the functioning of microalgae cells, while alkaline conditions reduce the ability to assimilate carbon dioxide. Research indicates that for most microalgae, the optimal pH range is between 6 and 10 [18]. Optimization of cultivation conditions is crucial for industrial productivity of microalgae. The objective aim of this work was to analyze some of the parameters important for algal biomass production and methods suitable for estimating Chlorella vulgaris growth. In this study, we hypothesized that optimization of basic culture conditions leads to higher biomass concentration in the photobioreactors.

Material
Chlorella vulgaris microalgae (BA 002) were used in this research. The material was obtained from Culture Collection of Baltic Algae (CCBA) (University of Gdansk, Gdańsk, Poland).
Algae cells were kept suspended by mixing with gas using a 25 W pump (Aqua Medic, Bissendorf, Germany) with a capacity of 0.9 m −3 ·h −1 . At the same time, the aeration made it possible to introduce carbon dioxide into the culture. The experiment was carried out as a batch culture. To establish an adequately fast and accurate method for the quantification of microalgae biomass, three comparative methods were used: biomass dry weight, measuring the content of chlorophyll a, and counting the number of cells using a hemocytometer.
Algae cells were kept suspended by mixing with gas using a 25 W pump (Aqu Medic, Bissendorf, Germany) with a capacity of 0.9 m −3 ·h −1 . At the same time, the aeratio made it possible to introduce carbon dioxide into the culture. The experiment was carrie out as a batch culture. To establish an adequately fast and accurate method for the quan tification of microalgae biomass, three comparative methods were used: biomass dr weight, measuring the content of chlorophyll a, and counting the number of cells using hemocytometer. The dry weight of microalgae has been determined by the gravimetric method de scribed by Ratha et al. [20] with modification. Biomass estimation was carried out using moisture analyzer (AXIS ATS, Gdańsk, Poland). Aliquots of 40 cm 3 were centrifuged (Ep pendorf, Hamburg, Germany) at 4000× g for 15 min. The biomass after centrifugation wa transferred to aluminum plates, dried at 105 °C to constant weight, cooled, and weighed The results were calculated and presented in mg·L −1 . The amount of biomass was deter mined on the day the culture was established and at 24-h intervals for the next 15 day The biomass productivity was calculated by the following equation where BP is the biomass productivity (mg·L −1 ·d −1 ), Bf and B0 are final and initial biomas concentration (mg), respectively, and d is the cultivation time (day). Chlorophyll concentration was determined according to the PN-86 C-05560/02 stand ard [21]. The pigment was extracted from biomass concentrated by filtration on a glass fibe filter using 90% (v/v) acetone. The absorbance of acetone extract was measured at wave length of 665 nm, with a spectrophotometer (EMCO, Warszawa, Poland). The results ar given in mg m −3 . The chlorophyll productivity was calculated using following equation: The dry weight of microalgae has been determined by the gravimetric method described by Ratha et al. [20] with modification. Biomass estimation was carried out using a moisture analyzer (AXIS ATS, Gdańsk, Poland). Aliquots of 40 cm 3 were centrifuged (Eppendorf, Hamburg, Germany) at 4000× g for 15 min. The biomass after centrifugation was transferred to aluminum plates, dried at 105 • C to constant weight, cooled, and weighed. The results were calculated and presented in mg·L −1 . The amount of biomass was determined on the day the culture was established and at 24-h intervals for the next 15 days. The biomass productivity was calculated by the following equation where BP is the biomass productivity (mg·L −1 ·d −1 ), B f and B 0 are final and initial biomass concentration (mg), respectively, and d is the cultivation time (day). Chlorophyll concentration was determined according to the PN-86 C-05560/02 standard [21]. The pigment was extracted from biomass concentrated by filtration on a glass fiber filter using 90% (v/v) acetone. The absorbance of acetone extract was measured at wavelength of 665 nm, with a spectrophotometer (EMCO, Warszawa, Poland). The results are given in mg m −3 . The chlorophyll productivity was calculated using following equation: where ChP is chlorophyll productivity (mg·m −3 ·d −1 ), Ch f and Ch 0 are final and initial content of chlorophyll (mg), respectively, and d is the cultivation time (day). Microalgal cells were counted using Thoma hemacytometer [22]. Two chambers of the hemacytometer were filled up with culture samples. Cells were counted under a light microscope (Delta Optical Genetic Pro Bino, Warszawa, Poland). The average cell count was multiplied by 10,000 to calculate the number of cells per milliliter of the microalgal culture.
The chlorophyll concentration and cell counting analyzes were performed on the first day of the experiment and then after 5, 10, and 15 days.

Statistical Analysis
All determinations were performed in triplicate. The results were statistically analyzed using the statistical software package for Windows (Statistica version 13.3; Dell Inc., Tulsa, OK, USA). Two-way analysis of variance was used. The significance of differences between the means was assessed using post hoc Duncan test at statistical significance of p ≤ 0.05. The standard deviation was also determined.

Results and Discussion
Various methods are used to assess the efficiency of biomass production. The most popular of them are the determination of dry matter [23], measurement of optical density, determination of chlorophyll content using a spectrophotometer, and a method based on counting cells using a counting chamber [24]. In the presented work, differences between the optimal process conditions determined with the use of individual methods were noted. Based on the statistical analysis of the test results, it was found that the photoperiod used, and the pH of the culture medium had a significant effect on the amount of biomass and the content of chlorophyll a in microalgae cells of the C. vulgaris species. However, the factors analyzed in the study did not have a significant effect on the number of microalgae cells.
The amount of biomass in individual photobioreactors at the beginning of the experiment was on average 417 ± 14.43 mg·l −1 . Under LED lighting conditions, the time of the culture entering the stationary growth phase occurred slightly earlier than under SON-T Agro lighting ( Figure 2). Depending on other factors, it was generally between the 7th and 9th day of breeding. Similar dependencies in their research were presented by Kim et al. [25]), who assessed the optimal conditions for the production of microalgae Chlorella sp., Dunaliella salina, and Dunaliella sp. biomass and found that the biomass content increased by the 8th measurement day. Similar results were obtained by Hawrot et al. [26] in a study on the production of Chlorella minutissima biomass using aquaculture wastewater. After 10 days, the breeding entered the stationary phase. The inhibition of the growth and development of microalgae between 8 and 10 days may be related to the depletion of nutrients in the culture medium [27].
The average amount of biomass in the culture with SON-T Agro illumination ranged from 506 ± 6.74 to 546 ± 7.88 mg·L −1 (Figure 4). The concentration of biomass in the culture depends on the initial inoculum density, but the amount of biomass obtained in the presented studies on the F/2 substrate was within the range of the results obtained by other authors. Matos et al. [29] in the modified BBM medium obtained 0.59 g·L −1 of biomass. Similar results were presented by Travieso et al. [30], who cultivated microalgae biomass in diluted nitrogen-rich waste and obtained a maximum of 510 mg·L −1 . The highest amount was determined in a photobioreactor illuminated for 18 h and at pH 7. Similar results were obtained by Wahidin et al. [31], who investigated the effect of the irradiation cycle on the growth of biomass of Nannochloropsis sp. In addition, the highest values were recorded in 18/6 h cycle. In studies conducted by Amini et al. [32], the highest increase in the biomass of microalgae Chlorella vulgaris was recorded in the 16/8 h (light/dark) photoperiod. Different results are presented by Atta et al. [33], who found in their research that the optimal culture conditions for the microalgae C. vulgaris occurred in the 12/12 h photoperiod (light/dark), while the lowest biomass increases with continuous lighting of the culture.
Appl. Sci. 2021, 11, x FOR PEER REVIEW 5 of 14 results were obtained by Wahidin et al. [31], who investigated the effect of the irradiation cycle on the growth of biomass of Nannochloropsis sp. In addition, the highest values were recorded in 18/6 h cycle. In studies conducted by Amini et al. [32], the highest increase in the biomass of microalgae Chlorella vulgaris was recorded in the 16/8 h (light/dark) photoperiod. Different results are presented by Atta et al. [33], who found in their research that the optimal culture conditions for the microalgae C. vulgaris occurred in the 12/12 h photoperiod (light/dark), while the lowest biomass increases with continuous lighting of the culture.
A B  Under LED light conditions, the average amount of biomass ranged from 489 ± 5.00 to 543 ± 1.92 mg·L −1 . As in the case of sodium lighting, the highest values were recorded in the 18/6 h cycle and pH 7. The amount of biomass obtained in a culture medium with a neutral pH, regardless of the type of lighting and the time of lighting culture, was generally significantly different from the other values. Khalil et al. [34] investigated the effect of pH on the growth of Dunaliella bardawil and Chlorella ellipsoidea and found that although these microalgae could grow over a wide pH range from 4 to 9/10, the greatest dry matter gain for D. bardawil was at pH 7.5, and for C. ellipsoidea, at alkaline pH (9-10). In a study by Qiu et al. [35], the highest increase in Chlorella sorokiniana biomass was obtained at pH 6. These results indicate the need to conduct research on the optimization of biomass production conditions, which change significantly depending on the type of microalgae.
According to Gong et al. [36], it is very important to keep the pH constant during cultivation. Methods with control of optimal pH value increased the growth of microalgae by over 56%. Chlorophyll content at the beginning of the experiment in all objects was 46 ± 6.17 mg·m −3 ( Figure 5). In the photobioreactors illuminated by SON-T Agro light, the highest values were determined on the fifth day of the experiment and a significant decrease in  Under LED light conditions, the average amount of biomass ranged from 489 ± 5.00 to 543 ± 1.92 mg·L −1 . As in the case of sodium lighting, the highest values were recorded in the 18/6 h cycle and pH 7. The amount of biomass obtained in a culture medium with a neutral pH, regardless of the type of lighting and the time of lighting culture, was generally significantly different from the other values. Khalil et al. [34] investigated the effect of pH on the growth of Dunaliella bardawil and Chlorella ellipsoidea and found that although these microalgae could grow over a wide pH range from 4 to 9/10, the greatest dry matter gain for D. bardawil was at pH 7.5, and for C. ellipsoidea, at alkaline pH (9-10). In a study by Qiu et al. [35], the highest increase in Chlorella sorokiniana biomass was obtained at pH 6. These results indicate the need to conduct research on the optimization of biomass production conditions, which change significantly depending on the type of microalgae.
According to Gong et al. [36], it is very important to keep the pH constant during cultivation. Methods with control of optimal pH value increased the growth of microalgae by over 56%. Chlorophyll content at the beginning of the experiment in all objects was 46 ± 6.17 mg·m −3 ( Figure 5). In the photobioreactors illuminated by SON-T Agro light, the highest values were determined on the fifth day of the experiment and a significant decrease in Under LED light conditions, the average amount of biomass ranged from 489 ± 5.00 to 543 ± 1.92 mg·L −1 . As in the case of sodium lighting, the highest values were recorded in the 18/6 h cycle and pH 7. The amount of biomass obtained in a culture medium with a neutral pH, regardless of the type of lighting and the time of lighting culture, was generally significantly different from the other values. Khalil et al. [34] investigated the effect of pH on the growth of Dunaliella bardawil and Chlorella ellipsoidea and found that although these microalgae could grow over a wide pH range from 4 to 9/10, the greatest dry matter gain for D. bardawil was at pH 7.5, and for C. ellipsoidea, at alkaline pH (9-10). In a study by Qiu et al. [35], the highest increase in Chlorella sorokiniana biomass was obtained at pH 6. These results indicate the need to conduct research on the optimization of biomass production conditions, which change significantly depending on the type of microalgae.
According to Gong et al. [36], it is very important to keep the pH constant during cultivation. Methods with control of optimal pH value increased the growth of microalgae by over 56%.
Chlorophyll content at the beginning of the experiment in all objects was 46 ± 6.17 mg·m −3 ( Figure 5). In the photobioreactors illuminated by SON-T Agro light, the highest values were determined on the fifth day of the experiment and a significant decrease in the content of chlorophyll and in microalgae cells in subsequent dates. Similar relationships were presented by Lakaniemi et al. [37] who, while examining the growth of C. vulgaris in tubular photobioreactors, found that in the initial phase of growth, the content of chlorophyll a increased, and then, after the 5th day of the experiment, the amount of assimilation pigment decreased almost to zero. Such a significant decrease in chlorophyll content, according to these authors, was related to the reduction of nutrients in the culture medium. In the presented research, a similar situation was observed in the conditions of cultivating with LED light. A significant increase in the content of chlorophyll was observed already with the second measuring time. The highest content of assimilated pigment was determined in the photobioreactor at 18/6 h cycle and pH 7. Similar results were obtained by Eriksen et al. [38] while breeding Chlamydomonas reinhardtii and Chlorella sp. in a gas-tight photobioreactor, and Perner-Nochta et al. [39] conducted a photoautotrophic culture of C. vulgaris. According to Lakaniemi et al. [37], determining the content of chlorophyll a is not a useful measure of biomass concentration due to the lack of correlation between the content of chlorophyll and the optical density of the culture. The content of chlorophyll a may also be misleading due to the differences in the size of the analyzed cells [40].  The highest chlorophyll productivity was on day 5 ( Figure 6). Content of microalgal chlorophyll in optimal condition was 24.21 ± 8.89 and 26.34 ± 2.01 mg·m −3 ·d −1 , under HPS and LED lightening, respectively.   The mean content of chlorophyll a with SON-T Agro illumination ranged from 70 + 10.11 to 135± 6.17 mg·L −1 (Figure 7). The highest content of assimilated pigment for this type of light was determined in a photobioreactor illuminated for 18 h and at pH 7. In the study by Khalil et al. [33], two strains of Dunaliella bardawil and Chlorella ellipsoidea at different pH variants showed that the neutral pH of the culture medium favored the accumulation of chlorophyll a and other assimilation pigments. The pH of the culture medium affects the entire process of microalgae cultivation. Unfavorable pH values influence the efficiency of nutrient absorption and metabolite production [41]. Additionally, pH conditions can cause calcium precipitation in some microalgae cultures [42]. Del Campo [43] found that the optimal pH for photosynthesis is generally lower than the requirements for growth and showed that pH values ranging from 6 to 9 stimulate carotogenesis for Muriellopsis sp. Under LED lighting, the mean amount of chlorophyll ranged from 62 ± 6.72 to 136 ± 14.13 mg·L −1 . Contrary to sodium lighting, the highest values were obtained at the photoperiod 12/12 and pH 6. No significant differences were found between the content of chlorophyll in microalgae cells at the highest values of chlorophyll a. The study of Yan et al. [44] on the effect of different wavelengths of LED light on the cultivation of C. vulgaris confirmed the effectiveness of this type of lighting in the production of biomass from algae; the use of red light has a positive effect on the content of chlorophyll a. Mohsenpour et al. [45] confirmed the dependence of red light on the increase in chlorophyll content and in C. vulgaris cells and the decrease in biomass content.
The initial number of microalgae cells in the culture was 9.3 × 10 5 (Figure 8). A significant increase in the number was recorded between the first and second measurement dates for SON-T Agro and LED light-illuminated photobioreactors. After this measurement date, the culture entered the stationary growth phase. With LED illumination at the end of the experiment in the two photoperiods, the culture had already reached the death phase. Many authors have shown a similar relationship. La et al. [46], examining the effect of glucose on the growth of C. vulgaris, showed that on the fifth day of the experiment, cell growth entered the stationary phase (20 × 10 6 of microalgal cells). The studies of Pagnanelli et al. [47] on the effect of the addition of nitrate and glucose to the culture medium on the growth of C. vulgaris and Nannochloropsis oculata cells showed, as in the presented work, a large increase in the initial phase of the experiment. The number of C. vulgaris cells ranged from 9.4 × 10 9 to 12 × 10 9 . This was due to the addition of nutrients to the medium, which stimulates the growth and development of microalgae [48]. On the other hand, studies by Glacio et al. [49], concerning the effect of nutrients on the growth of C. vulgaris biomass, showed the growth of algae cells only up to the 8th day of measurement, after which the culture entered the death phase.   The mean values of the number of microalgae cells indicate that the time of lighting culture, and the pH had no significant effect on the obtained values ( Figure 9). Under SON-T Agro lighting, the highest values were recorded at the 18/6 h photoperiod (19.3 × 10 5 -20.8 × 10 5 ), and under LED lighting, the highest average number of microalgae cells was recorded at 12/12 h (18.9 × 10 5 -20.8 × 10 5 ), however, as shown in Figure 5, they did not differ significantly from other lighting conditions. There was also no significant influence of medium pH on the analyzed parameters. On the other hand, the research by Vaičiulytė et al. [50] were carried out under constant lighting conditions and results showed significant differences in the number of C. vulgaris cells depending on cultivation conditions (batch growth or semi-continuous culture) and types of culture media. The analysis of the number of cells used to quantify the biomass does not define the stage of development of the culture and the size of the cells [51].
A positive correlation was found between the results of different methods of biomass determination in culture, however, a high R-factor was obtained mainly between the gravimetric measurement and the determination of the number of microalgae cells in the Thoma chamber, regardless of the type of lighting (r = 0.76 for sodium light and r = 0.83 for LED light). Based on the results of own research and the results of other authors [52], it can be concluded that the chlorophyll measurement method is not the best indicator of the estimation of biomass. Chlorophyll is a nitrogen-rich compound [53]. During cultivation, under N depletion in culture medium, chlorophyll is degraded to utilize nitrogen [54]. The chlorophyll content will decrease, while the amount of biomass may increase. The direct methods are more reliable. According to Rath et al. [20], such a method is a gravimetric measurement performed with a moisture analyzer, similarly to the presented work. This method is not only accurate but also much faster compared to the analyzes performed by traditional biomass drying in the oven. The mean values of the number of microalgae cells indicate that the time of lighting culture, and the pH had no significant effect on the obtained values ( Figure 9). Under SON-T Agro lighting, the highest values were recorded at the 18/6 h photoperiod (19.3 × 10 5 -20.8 × 10 5 ), and under LED lighting, the highest average number of microalgae cells was recorded at 12/12 h (18.9 × 10 5 -20.8 × 10 5 ), however, as shown in Figure 5, they did tion, under N depletion in culture medium, chlorophyll is degraded to utilize nitrogen [54]. The chlorophyll content will decrease, while the amount of biomass may increase. The direct methods are more reliable. According to Rath et al. [20], such a method is a gravimetric measurement performed with a moisture analyzer, similarly to the presented work. This method is not only accurate but also much faster compared to the analyzes performed by traditional biomass drying in the oven.

Conclusions
The production of microalgae biomass requires the selection of optimal cultivation conditions. The most important parameters are the type and the time of lighting, which will also be important for the economy of the process. All factors used in the research had a significant impact on the amount of biomass. Depending on the method used (direct, indirect), different results were obtained. For dry mass measurement, the optimal culture conditions were recorded using 18/6 h light/dark cycle and pH of 7-546 ± 7.88 mg·L −1 for high-pressure sodium light and 543 ± 1.92 mg·L −1 for light-emitting diode. The maximum content of chlorophyll a was determined in the 12/12 h light/dark cycle, pH 6, under lightemitting diode (136 ± 14.13 mg m −3 ) and 18/6 h, pH 7, under sodium light (135 ± 6.17 mg m −3 ). The biomass and chlorophyll productivity in optimal conditions under HPS and LED light was 27.08± 7.80 and 24.21 ± 8.89 mg·m −3 ·d −1 and 25.00± 5.1 and 26.34 ± 2.01 mg·m −3 ·d −1 , respectively. The highest number of microalgae cells (2.1 × 10 6 ) was obtained at pH 7 and photoperiod 18/6 h under sodium light, and 12/12 h under light-emitting diode. The results for all measurements suggest that particular methods can be used alternately during

Conclusions
The production of microalgae biomass requires the selection of optimal cultivation conditions. The most important parameters are the type and the time of lighting, which will also be important for the economy of the process. All factors used in the research had a significant impact on the amount of biomass. Depending on the method used (direct, indirect), different results were obtained. For dry mass measurement, the optimal culture conditions were recorded using 18/6 h light/dark cycle and pH of 7-546 ± 7.88 mg·L −1 for high-pressure sodium light and 543 ± 1.92 mg·L −1 for light-emitting diode. The maximum content of chlorophyll a was determined in the 12/12 h light/dark cycle, pH 6, under light-emitting diode (136 ± 14.13 mg m −3 ) and 18/6 h, pH 7, under sodium light (135 ± 6.17 mg m −3 ). The biomass and chlorophyll productivity in optimal conditions under HPS and LED light was 27.08± 7.80 and 24.21 ± 8.89 mg·m −3 ·d −1 and 25.00 ± 5.1 and 26.34 ± 2.01 mg·m −3 ·d −1 , respectively. The highest number of microalgae cells (2.1 × 10 6 ) was obtained at pH 7 and photoperiod 18/6 h under sodium light, and 12/12 h under light-emitting diode. The results for all measurements suggest that particular methods can be used alternately during the determination of biomass content. However, if other indicators (cell number and chlorophyll a content) are used for biomass validation, the optimal culture parameters may change with different types of light. Considering the results, the most accurate method for biomass evaluation is gravimetric dry weight assessment > cell counting > chlorophyll content.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.

Conflicts of Interest:
The authors declare no conflict of interest.

Abbreviations
LED light light-emitting diode HPS light high-pressure sodium light