Sequential Extraction Analysis of Arsenic in Soil Samples Collected in an Agricultural Area of Brindisi, Apulia (Italy), in the Proximity of a Coal-Burning Power Plant

: In order to evaluate the local environmental risk deriving from coal transport and burning, 30 soil samples were collected along the coal conveyor belt and around the coal-burning power plant Federico II of Brindisi (Apulia, southern Italy). Since soil pollution of metals and metalloids is measured not only by their total concentration but also by evaluating their mobility and availability, arsenic sequential extraction, leaching test and speciation analysis were performed. The analytical results showed that the most abundant As amount was contained in the ﬁnal residue of the sequential extraction procedure, the so-called occluded As, embedded in rock formations such as sulphides. Moreover speciation chemical analysis showed that As(V) was by far the most abundant species, whereas As(III), which represents the most soluble and available species, was only a very small fraction. No arsenic organic species were detected in the soil samples.


Introduction
Arsenic is relatively scarce in the natural environment (20th position in elemental abundances on the continental crust), but it is widely distributed. It can be found in detectable amounts in nearly all rocks and soils. As in soils relates to the geological substratum and consequently a rather wide range of As levels have been reported in soils around the world with an average of 5-10 mg/kg in uncontaminated soils [1]. However, As may accumulate in soils due to human activities such as waste discharges of metal processing plants, burning of fossil fuels, mining of As containing ores and agricultural use of arsenical pesticides. In this respect it is important to know that As has different degrees of toxicity depending on the speciation of As, dose of exposure, individual and local tissue susceptibilities [2]. The oxidation state of As is important since it affects movement, persistence and toxicity in soils and sediments. In oxygen-rich environments and well-drained soils, arsenate (AsO 4 3− ) species dominate, whereas under reducing conditions, such as regularly flooded soils, arsenite (AsO 3 3− ) is the stable oxidation state, but elemental As and arsine (AsH 3 ) can also be present in strongly reducing environments. As may also exist in organometallic forms such as monomethylarsonic acid (MMAA, CH 5 AsO 3 ), dimethylarsinic acid (DMAA, C 2 H 7 AsO 2 ) and the more volatile methylarsines. Iron, aluminum and calcium arsenates are, like the corresponding phosphates, virtually insoluble in water and arsenate exhibits a phosphate-like specific adsorption (inner sphere complexation) on oxide and clay surfaces. Arsenites, however, are 10 times more soluble and mobile and are, furthermore, more toxic than arsenates [3]. Table 1. As concentrations detected in the 5 different depth levels of the core extracted during a previous investigation.
Different sequential extraction schemes have been designed for the analysis of metals in soils and sediments [10,21]. In this case, the speciation aims at understanding the distribution of a metal over the various sedimentary substrates such as carbonates, iron and manganese oxyhydroxides, organic matter, sulphides, silicates, etc. Under particular conditions some of these substrates will dissolve (for example the oxyhydroxides under reducing conditions and the carbonates under acidic conditions) or release adsorbed metals (for example when the electrolytic strength of the solution is increased). It is thus possible, by carefully selecting the composition of the extraction solutions, also called extractants, to destroy selectively specific soil or sediment substrates. Information obtained from sequential extraction procedures can be very interesting, although a prerequisite is that the analytical procedures are well-defined and accepted.
Moreover, in order to evaluate the As availability in soil samples we performed also a classic leaching test in acetic acid (the Istituto di ricerca sulle acque -Consiglio Nazionale delle Ricerche, IRSA-CNR, method): this assay analyzes the behaviour of a solid matrix (both organic and inorganic materials) towards the action of meteoric water. Finally a speciation analysis was performed by combining high-performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS) techniques.

Sample Collection
Thirty soil samples, coming from the agricultural area of Brindisi (Apulia, southern Italy), were collected along the coal conveyor belt and around the coal-burning power plant Federico II in the month of July 2008. In particular, the soil samples belonged to 5 cores extracted in a limited area where a previous investigation had detected very high values of As concentration (>1500 mg kg −1 , Table 1). Each core was 5 m deep and 6 different soil sample were derived from each one, corresponding to 6 different depth levels: 0.0-1.

Sequential Extractions
A sequential extraction procedure, consisting in five steps, was performed [22]. As the first step, 90 mL of ammonium acetate 1 M (adjusted to pH 5 with acetic acid) were added to 5 g of sediment, previously dried at room temperature. The samples were stirred for 24 h and then centrifuged to separate the extracted solution from the solid residue.

Sequential Extractions
A sequential extraction procedure, consisting in five steps, was performed [22]. As the first step, 90 mL of ammonium acetate 1 M (adjusted to pH 5 with acetic acid) were added to 5 g of sediment, previously dried at room temperature. The samples were stirred for 24 h and then centrifuged to separate the extracted solution from the solid residue. Ammonium acetate allowed to extract the water-soluble fraction of metals and metalloids, bound as carbonates and highly exchangeable forms [23].
After at least two washings with distilled water, at the second step each sample was treated with 90 mL of hydroxylammine chloride 1 M (adjusted to pH 5 with sodium citrate) by stirring for 24 h. In these conditions, an improvement of As solubility, due to the increase of Fe and Mn oxides and hydroxides solubilization, was verified. In fact, there is a significant correlation between Fe solubility and As mobility thus confirming that Fe oxides and hydroxides are the main As adsorption agents [23,24]. It has been also reported that As mobility in soils mainly depends on complexation reactions with oxides and hydroxides of Al, Mn, and Fe [25]. Moreover, it is well known that Fe oxides and hydroxides are dominant in the adsorption process of As in soils: reducing and chelating agents cause the co-dissolution of As and oxides and hydroxides, making this metalloid more soluble and available [26]. The separation of the extracted solution and the washing of the solid residue were performed as already described in the first step.
At the third step of the sequential extraction procedure 25 mL of HCl 0.1 M were added to the solid residue of the previous step, stirring for 24 h. The forth extraction was performed by adding 25 mL of NaOH to each residue of the third step and stirring for 48 h. The third and the forth step allowed to extract metal fraction bound to the organic matter and the clay minerals. In fact, HCl breaks the bonds between clays and humic substances and NaOH extracts arsenates strongly adsorbed on the clay surface [26][27][28].
The solid residue of the fourth step was washed at least twice and then treated with 25 mL of HNO 3 8 M, stirring for 3 h at 85 • C. The extracted solution was separated by centrifugation and solid residue was washed at least twice with distilled water. The fifth step extracted the not easily available As fraction (for example, sulphide), but it was soluble in acid conditions and high temperature.
After the fifth step, the solid residue is stored for the following digestion and spectrometric analysis. So we obtained six different shares for each soil sample: five shares from the five steps of the sequential extraction procedure and one share from the final solid residue.
The As extracted at the fifth step of the sequential extractions and still present in the final solid residue is the so called "occluded As" [26], i.e., As incorporated in minerals that do not easily release the metalloid, for example some very insoluble sulphides such as arsenopyrite [27].
The solutions extracted from soil samples during the sequential extraction procedure and the final solid residue were analyzed by ICP-MS spectrometry (Environmental Protection Agency, EPA, method 6020). In particular, the final solid residues of the five step sequential extraction were digested by aqua regia (one volume of HNO 3 and three volumes of HCl) mineralization.
The final results were expressed in µg/kg (ppb) of dry weight and were referred to 1 kg of soil, dried at room temperature in order to avoid As, which forms very volatile species, being lost and the measurements being altered.

Leaching Test
A classic leaching test in acetic acid (IRSA-CNR method, APAT CNR IRSA 3020 Man 29 2003) was performed in order to evaluate the As availability in soil samples: this assay analyzes the behaviour of a solid matrix (both organic and inorganic materials) towards the action of meteoric water. We treated 2.5 g of each soil sample (particles with a size <2 mm dried at room temperature) with 50 mL of water (extraction ratio 1:20) for 24 h (adjusted to Appl. Sci. 2021, 11, 2115 5 of 14 pH 5 with diluted acetic acid). The resulting solution, filtered through 0.45 µm filters, were used for As determination by ICP-MS spectrometry.

Speciation Analysis
A speciation analysis was performed in order to determine As species in the soil samples: As(III), As(V), and organic fractions (MMAA, DMAA). 0.200 g of each sample, dried at room temperature and sifted to eliminate particles with dimensions >2 mm, were suspended in 50 mL of ammonium oxalate 0.2 M and then left in an ultrasonic bath for 10 min. The extracted As was detected by the combination of HPLC (Waters 626 LC System equipped with a ionic exchange Hamilton PRP-X100 column, 250 × 4.6 mm inner diameter), and ICP-MS (Varian ICP-MS 820-MS) techniques.

Instrumental Details
All determinations of As were performed on a Varian 820 MS inductively coupled plasma mass spectrometer (ICP-MS).

Sequential Extractions
Before the sequential extraction procedures, As total amount was determined by ICP-MS. 30 soil samples, corresponding to 6 depth levels (0.0-1.0, 1.0-1.5, 1.5-2.0, 2.0-3.0, 3.0-4.0, and 4.0-5.0 m) of 5 cores withdrawn from S53 sampling site and its close surroundings, were analyzed. Comparing S53 core results with the data reported by "Sviluppo Italia" (Table 2), we can note that As concentrations are significantly lower than amounts initially detected. In the superficial levels As amount determined by us (19.21) is similar to As concentrations reported by "Sviluppo Italia" (17.90 ppm), whereas between 1 m and 2 m of depth we reported lower As concentration values (11.89 and 15.23 ppm instead of 1520 ppm). In the depth level 2.0-3.0 m we detected 44.51 ppm instead of 84.30 ppm reported by "Sviluppo Italia" and in the deepest levels (3.0-4.0 and 4.0-5.0 m) As concentrations were slightly higher than "Sviluppo Italia" data (21.13 and 25.78 ppm instead of 15.90 and 9.89 ppm, respectively). Table 2. Comparison between the results relating to the determination of the total As reported by Sviluppo Italia (S. I.) and to the survey carried out by the University of Salento (U. S.), in the sampling point S53.

Sampling Point
Depth ( The results of As amount in the 5 cores of S53 sampling site (S53/1 core) and its close surroundings (S53/1A, S53/1B, S53/1C, S53/1D cores) are summarized in Table 3. In general, As showed a tendency to accumulate in the deepest soil levels. In fact, only for the core S53/1A was the highest As concentration detected on the surface, whereas the highest average value (~32 ppm) was detected in the 2.0-3.0 level.
The results of As sequential extractions are summarized in Table 4. For each soil sample, the sum of the As amount in the six fractions is compared with the total As concentration previously determined and it ranged between −12% and +8%. On average the most abundant amount of As was obtained from the residues of the sequential extraction procedures (44.1%) and it ranged between 25.0% (S53/1A core, depth 4.0-5.0 m) and 57.6% (S53/1A core, depth 2.0-3.0 m). As in the last residue was the most abundant fraction of total extracted As in 22 of the 30 soil samples (more than 73% of all samples). Table 3. Results for the total As in the six levels of the five cores of point S53 and its narrow surroundings. The last column shows the average values for each level of the soil cores. Values above the tolerability limit of 20 ppm, as required for areas intended for public green use and private and residential use by Decree of the Italian Environment Ministry n. 471 of 1999, are highlighted in bold.

Depth (m)
[As] (ppm) Also by comparing the average values of the 6 As fractions in the 6 depth levels, the last residue resulted the most abundant fraction 5 times out of 6: in fact, only in the level 4.0-5.0 m the most abundant As amount was obtained from the 5th step of the sequential extractions ( Figure 2). Also by comparing the average values of the 6 As fractions in the 6 depth levels, the last residue resulted the most abundant fraction 5 times out of 6: in fact, only in the level 4.0-5.0 m the most abundant As amount was obtained from the 5th step of the sequential extractions ( Figure 2). The first As fraction deriving from the sequential extraction consists in the most easily exchangeable and, therefore, the most available species of the metalloid. This fraction represented 2% of extracted As and ranges from 0.7% (core S53/1, depth 2.0-3.0 m) to 3.4% (core S53/1A, depth 3.0-4.0 m) ( Table 4).
From the third step onwards, extraction conditions were made severe by using strongly acid or alkaline extracting solutions. In particular in the third step HCl 0.1 M solution was used and this fraction was the less abundant (on average 0.5% of extracted As, Table 4).
As extracted in the fourth step of the sequential extraction procedures was on average 16.4% of total As and ranged from 4.3% (core S53/1D, depth 3.0-4.0 m) to 36.9% (core S53/1A, depth 1.0-1.5 m). The soil sample derived from the core S53/1A at the level 1.0-1.5 m was the only sample with an As concentration of the fourth fraction similar to As concentration of the final residue (4520 ppb and 4514 ppb, respectively) ( Table 4).
As extracted in the fifth step of sequential extractions was on average 28.4% of total extracted As and ranged between 8.4% (core S53/1D, depth 1.0-1.5 m) and 60.4% (core S53/1D, depth 3.0-4.0 m) ( Table 4). Metal and metalloid species extracted at this step are The first As fraction deriving from the sequential extraction consists in the most easily exchangeable and, therefore, the most available species of the metalloid. This fraction represented 2% of extracted As and ranges from 0.7% (core S53/1, depth 2.0-3.0 m) to 3.4% (core S53/1A, depth 3.0-4.0 m) ( Table 4).
From the third step onwards, extraction conditions were made severe by using strongly acid or alkaline extracting solutions. In particular in the third step HCl 0.1 M solution was used and this fraction was the less abundant (on average 0.5% of extracted As, Table 4).
As extracted in the fourth step of the sequential extraction procedures was on average 16.4% of total As and ranged from 4.3% (core S53/1D, depth 3.0-4.0 m) to 36.9% (core S53/1A, depth 1.0-1.5 m). The soil sample derived from the core S53/1A at the level 1.0-1.5 m was the only sample with an As concentration of the fourth fraction similar to As concentration of the final residue (4520 ppb and 4514 ppb, respectively) ( Table 4).
As extracted in the fifth step of sequential extractions was on average 28.4% of total extracted As and ranged between 8.4% (core S53/1D, depth 1.0-1.5 m) and 60.4% (core S53/1D, depth 3.0-4.0 m) ( Table 4). Metal and metalloid species extracted at this step are not easily available, in particular considering extraction conditions (HNO 3 8 M for 3 h at 85 • C). The fifth fraction was the most abundant in 7 out of 30 soil samples (23.3%) and represented by far the most abundant As share at the level between 4.0 and 5.0 m (Table 4).
After soil sample analysis, sequential extraction procedures were performed also for coal samples (Table 5). There was a good match between total As concentration, determined in a unique mineralization, and the sum of the fractions derived from sequential extractions. At the first and second steps extracted As was not detectable, whereas the highest concentrations were obtained at the fifth step (27.0%) and in the final residue (61.3%). In Figure 3 the profiles of arsenic released in the soil of the examined area are compared with the release profiles found in the coal samples provided by the ENEL power company, thus highlighting the similarities in the distribution of the fractions. In Figure 3 the profiles of arsenic released in the soil of the examined area are compared with the release profiles found in the coal samples provided by the ENEL power company, thus highlighting the similarities in the distribution of the fractions.

Leaching Test
The amounts of As extracted with acetic acid from the 30 soil samples were always lower than 1% of total As and ranged from 6 ppb (0.02% of total As; core S53/1D, depth 1.0-1.5 m) to 126 ppb (0.97% of total As; core S53/1A, depth 3.0-4.0 m, Table 6). In general, a progressive growth of extracted As with the increase of soil depth can be observed. In fact, the lowest values were detected in the superficial samples, whereas in the following three depth levels a restrained increase was observed. A substantial increase was detected in the deepest levels.

Leaching Test
The amounts of As extracted with acetic acid from the 30 soil samples were always lower than 1% of total As and ranged from 6 ppb (0.02% of total As; core S53/1D, depth 1.0-1.5 m) to 126 ppb (0.97% of total As; core S53/1A, depth 3.0-4.0 m, Table 6). In general, a progressive growth of extracted As with the increase of soil depth can be observed. In fact, the lowest values were detected in the superficial samples, whereas in the following three depth levels a restrained increase was observed. A substantial increase was detected in the deepest levels. Table 6. Leaching test data for As in the soil samples coming from the 5 cores of point S53, and its close surroundings. The values are reported in ppb (µg/kg dry weight) and as a percentage of the total extracted As.  Table 6. Cont.

Speciation Analysis
Ammonium oxalate 0.2 M extraction was used for the chemical speciation analysis of As, combining HPLC with ICP-MS techniques ( Figure 4). Generally, As extracted was almost entirely As(V) species and only a very low percentage of As(III) was measured. The organic species MMAA and DMAA were completely absent. Here, we show the results concerning the soil sample deriving from the S53/1D core (depth 4-5 m), because it was the sample with the highest As percentage extracted by ammonium oxalate 0.2 M (8.6%). In this sample As(V) was 96.9% of As extracted, whereas As(III) was 3.1%.

Discussion
The impact of environmental pollution due to metals and metalloids in soil depends not only on their total concentration but also on their mobility and availability, which affects their release and interaction with other environmental components, such as water, air, and vegetables. In order to evaluate metal mobility in soil, sequential extraction pro- Moreover, we used phosphoric acid 0.5 M, an extraction agent more effective than ammonium oxalate 0.2 M, on the same soil sample (S53/1D core, depth 4-5 m) in order to determine As species: As extracted was 21% of total As and As(V) was the only species present (data not shown).

Discussion
The impact of environmental pollution due to metals and metalloids in soil depends not only on their total concentration but also on their mobility and availability, which affects their release and interaction with other environmental components, such as water, air, and vegetables. In order to evaluate metal mobility in soil, sequential extraction procedures can be performed, in which a soil sample is treated with solutions with different extractant capacity that provoke the release of metal rates characterized by different solubility and, consequently, mobility. The fractions extracted by weak reagents are more mobile and dangerous from an environmental point of view, because they represent the metal portion which is released more easily from soil to other environmental compartments, such as superficial and ground water, or adsorbed by vegetables which grow in the contaminated site. In soil As is adsorbed mainly by minerals characterized by variable charge: in fact, it was observed by EXAFS methodologies, that AsO 4 3− ions were adsorbed on the surface of minerals with variable charge forming "inner-sphere complexes", characterized by very strong covalent bonds [29]. These bonds can be mono-or bidentate mononuclear and/or bidentate binuclear in different proportions according to the characteristics of the absorbent surfaces. Recent studies showed that AsO 4 3− ions are adsorbed more efficiently by Mn and Fe oxides than Al oxides, whereas AsO 3 3− ions are strongly adsorbed by Fe oxides and weakly adsorbed by Al oxides [30][31][32][33][34].
Sixteen of the 30 soil samples taken at the studied area were characterized by an As concentration higher than 20 ppm, the limit set by the 471/99 D.M. in the areas for public parks and private and residential use, and the highest value was 44.51 ppm (S53/1 core, depth 2.0-3.0 m). No soil sample showed an As amount higher than 50 ppm, the limit set by the 471/99 D.M. in the commercial and industrial areas.
As extracted in the first step of the sequential extraction procedures was the less abundant in all the analyzed soil sample. Even if sequential extraction methods can change significantly, the first step is always performed in less drastic conditions and allows to solubilize the most available fraction of analyte and, therefore, the rate of metal or metalloid with the highest potential toxicity. Also As extracted in the second step is significantly bioavailable, even if in these specific extraction conditions As species are not so water-soluble like As forms extracted in the first step.
In general, As extracted at the fourth step represented an abundant fraction of total As in all analyzed soil samples. The organic matter has an important role in determining As mobility: the adsorption of As(III) and As(V) on humic acids is influenced by pH and the concentration of the element. Humic acids could be important for As adsorption in relatively acid environments, whereas in alkaline environments As release and solubilization are favored [35]. Sodium hydroxide, used at the fourth step of the sequential extractions, is able to solubilize the organic matter, as pointed out by the darkening of the extracts from the superficial soil samples, where we expect a higher concentration of humic substances. But the As fraction extracted at the fourth step also includes arsenates bound to Fe-rich minerals and, in general, to clay matrices [27].
As species extracted at the fifth step and in the final residue (dissolved in aqua regia) form the so called As occluded [26], As included in minerals such as sulphides. However, we also have to consider that in the superficial levels sulphide oxidation is possible due to the metabolism of the bacterial species present in the soil, and consequently the transformation of As in more available forms [36]. This hypothesis is not applicable to the deepest levels due to the anaerobic conditions which characterize these depths. The collected data for the soil samples from the S53 sampling point and its close surroundings suggest that As is present mainly in an occluded form. Figure 4 describes the comparison between the As amount extracted by sequential extractions and the As quantity extracted by ammonium oxalate 0.2 M in the chemical speciation procedure. In general, As extracted with ammonium oxalate was always less than the sum of the As fractions extracted in the first and second step of sequential extractions. Moreover, in the superficial levels As extracted by oxalate was very similar to that extracted in the first step of sequential extractions, whereas when increasing the depth of the soil sample As extracted by oxalate became more abundant.
As extraction by ammonium oxalate for the chemical speciation analysis could be considered like a leaching test which allows the release of the highest possible amount of As, without changing his chemical properties. Analyzing As leaching in ammonium oxalate 0.2 M, we noticed that the highest levels of As release was obtained from the deepest soil sample, with a maximum generally between 2 and 3 m of depth (core S53/1, S53/1A e S53/1B). The As amount extracted from soil samples of S53 sampling point and its surroundings was on average 4% of total As. This value was very different from As extracted by ammonium oxalate from other areas located near the site analyzed in this study. For example, in the Sviluppo Industriale e dei Servizi Reali alle Imprese (SISRI) area of Brindisi, As extracted by ammonium oxalate 0.2 M was 62% of total As. Comparing As amounts extracted by acetic acid with As extracted by ammonium oxalate, we observed much higher values of As extracted for the chemical speciation analysis. In fact, As extracted by acetic acid ranges from 6 to 126 ppb, whereas As extracted by ammonium oxalate ranges from 152 to 1962 ppb.
In this regard, Figures 5 and 6 show the comparison between the average percentage of As extracted for the leaching test by ammonium oxalate and in the first two steps and the first four steps of sequential extraction procedures for soil samples in the most superficial layers on the one hand, and the corresponding average values of all levels of depth on the other hand. For total extracted As values in the first four steps of the sequential extraction only, the averages found in the upper layer were higher than the averages measured in all the other levels of depth. This is probably due to the increased presence of organic matter in the upper layer, the matrix on which some of the As species extracted by NaOH in the fourth step of the sequential extraction are adsorbed. Further studies concerning the determination of As release due to the removal of the organic substance performed in the third and the fourth step of the sequential extraction and due to the possible use of fertilizers, especially phosphates with an anionic component with high exchange capacity for As oxyanions, are needed.
Appl. Sci. 2021, 11, x FOR PEER REVIEW 12 of 15 much higher values of As extracted for the chemical speciation analysis. In fact, As extracted by acetic acid ranges from 6 to 126 ppb, whereas As extracted by ammonium oxalate ranges from 152 to 1962 ppb. In this regard, Figures 5 and 6 show the comparison between the average percentage of As extracted for the leaching test by ammonium oxalate and in the first two steps and the first four steps of sequential extraction procedures for soil samples in the most superficial layers on the one hand, and the corresponding average values of all levels of depth on the other hand. For total extracted As values in the first four steps of the sequential extraction only, the averages found in the upper layer were higher than the averages measured in all the other levels of depth. This is probably due to the increased presence of organic matter in the upper layer, the matrix on which some of the As species extracted by NaOH in the fourth step of the sequential extraction are adsorbed. Further studies concerning the determination of As release due to the removal of the organic substance performed in the third and the fourth step of the sequential extraction and due to the possible use of fertilizers, especially phosphates with an anionic component with high exchange capacity for As oxyanions, are needed.   Comparison between the average percentage of As extracted for the leaching test, by ammonium oxalate, and in the first two steps and the first four steps of sequential extraction procedures for soil samples in the most superficial layer. Figure 6. Comparison between the average percentage of As extracted for the leaching test, by ammonium oxalate, and in the first two steps and the first four steps of sequential extraction procedures for soil samples, in all the sampled levels of depth.

Conclusions
Total As determination in the 30 soil samples deriving from the 5 probes of the S53 sampling point and its surroundings showed that As concentration was lower than the As amount detected by "Sviluppo Italia". In general, As accumulates in the deepest depth Figure 6. Comparison between the average percentage of As extracted for the leaching test, by ammonium oxalate, and in the first two steps and the first four steps of sequential extraction procedures for soil samples, in all the sampled levels of depth.

Conclusions
Total As determination in the 30 soil samples deriving from the 5 probes of the S53 sampling point and its surroundings showed that As concentration was lower than the As amount detected by "Sviluppo Italia". In general, As accumulates in the deepest depth levels. In 16 of the 30 soil samples As concentration exceeds 20 ppm limit, established by D.M. 471/99 for green areas for public use and private and residential use, and in no case did As concentration exceed the limit of 50 ppm, specified by the same decree for commercial and industrial areas.
The As sequential extraction protocol showed that the most abundant As amount was contained in the final residue of the sequential extraction procedure. As extracted in the fifth step and As in the final residue is the so-called occluded As that is embedded in rock formations that do not release the metalloid, including some very insoluble sulphides such as arsenopyrite.
The graphs showed in this work summarize the different scenarios proposed by us for an evaluation at different levels of approximation of environmental risk related to the presence of As, if the total concentration is not taken into account. This is because the environmental impact of pollution by metals and metalloids in soils depends not only on their total concentration but also on their mobility and availability.
The first scenario is derived from considering for risk evaluation only the As extracted by acetic acid in the leaching test. This test allows us to evaluate the amount of extractable As from the leaching effect of rain water. As also described above, the amount of As extracted by acetic acid was very limited, an order of magnitude lower than As extracted by ammonium acetate in the first step of sequential extractions and by ammonium oxalate in the speciation analysis. It was on average 0.16%, both of total As obtained in a unique digestion and total As extracted by the sequential extraction protocol.
A more cautious approach may lead to consideration for risk evaluation the amount of As obtained in the first two steps of sequential extractions. This amounts to an average of 9.8% of total As determined in a single mineralization and to 10.5% of As extracted in the sequential extraction procedures. The second proposed scenario also takes into account the As extracted by ammonium oxalate, used for the analysis of chemical speciation and which consists in the percentage of As extracted in mild conditions that do not change the percentages of As(III), As(V) and organic species possibly present. As extracted by 0.2 M ammonium oxalate is on average 4% of total As extracted in the sequential extractions and 3.9% of total As determined in a single digestion. These values are still below the sum of the percentages of As extracted in the first two steps of sequential extractions.
Another scenario is derived from considering the As extracted in the first four steps of sequential extractions. We should consider that the third and fourth steps, although carried out using "strong" extractants (0.1 M HCl and 0.5 M NaOH) hard to find in natural conditions, mobilize the arsenates bound to the surface of minerals rich in Fe and, in general, to clay and, if present, As bound to organic matrices, which in the more superficial layers of soil can be a substantial fraction.
Our results showed that only for total extracted As values in the first four steps of the sequential extraction are the averages found in the upper layer higher than the averages measured in all the other levels of depth, and we attributed this effect to a greater amount of organic matter in the upper layer, a matrix certainly capable of absorbing some of the As species extracted by NaOH in the fourth step of the sequential extraction.
As extracted in the studied area was characterized by different chemical species as compared with As from other areas of the Sito di Interesse Nazionale National Interest Site, SIN) of Brindisi. In fact, in the soil sample collected in the agricultural area of Brindisi, along the coal conveyor belt and around the coal-burning power plant Federico II, As fraction extracted by ammonium oxalate 0.2 M was on average 4.0% and ranged from 0.8% to 8.6%, whereas in the SISRI area As extracted by ammonium oxalate was 62% (35.9-91.7%). However, the percentage of As extracted by ammonium oxalate was always higher than the As amount extracted by ammonium acetate at the first step of sequential extractions.
In the soil samples from S53 sampling point and its close surroundings speciation chemical analysis showed that As(V) was by far the most abundant species, whereas As(III) was only a very little fraction. Organic species were completely absent. The result of studies performed in a very close area (such as the SISRI area) have already shown that As(V) was more than 90% of extracted As, whereas As(III), which represents the most soluble and available species, was less than 10%. Funding: This research has been performed in the framework of the project: "Analisi di rischio sul lotto di aree agricole adiacente al nastro trasportatore ENEL ed alla Centrale Federico II caratterizzate in stralcio al Piano di caratterizzazione delle aree agricole" financed by Regione Puglia.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are included in this published article.