Non-Thermal Intervention of Lung Tumor by Core-Shell Magnetic Nanoparticles in a Magnetic Field

Featured Application: The options for the treatment of K-Ras-driven lung tumors are currently limited. This study investigates the potential for non-thermal intervention against lung tumors using magnetic nanoparticles (MNPs) in a magnetic ﬁeld. The MNPs for use in treating tumors were prepared by chemical methods. Simulations showed that the experimental platform can generate the desired magnetic ﬁeld by avoiding any damaging rise in temperature and that the induced magnetic force can affect the cellular activities. Using MNPs in a magnetic ﬁeld in this way can not only suppress the growth of tumor cells but also can strengthen the inhibitory effects of magnetic ﬁeld stimulation on the lung tumors caused by the K-Ras mutations. Abstract: K-Ras mutations result in normal cells dividing uncontrollably and becoming cancerous. The prognosis is currently poor for patients due to the lack of drugs that can effectively target these mutations. In this study, magnetic nanoparticles (MNPs) were prepared, characterized, and cooperated with a magnetic ﬁeld to intervene in the growth of lung tumor cells. The rise in temperature of a stimulation coil was studied by numerical calculation. The non-thermal effects of MNPs under a magnetic force were analyzed. The cell experiments showed that the growth of A549 tumor cells slowed down. The result of a wound-healing assay also indicated that the migration of tumor cells was suppressed. Compared with magnetic stimulation without MNPs, MNPs enhanced the inhibitory effects of a magnetic ﬁeld. This study suggests a new way to treat K-Ras driven lung tumors using non-thermal effects of MNPs without the side effects caused by thermal effects. the biochemical and properties to tyro-sinase, of this model to study the mechanis-tics and kinetics of inhibition in response to various compounds. Targeting of the human tyrosinase is the main approach to limit the over biogenesis of melanin and its hazardous derivatives, thus controlling the various pathogenetic disorders. The activ-ity of the purified tyrosinase by kojic acid and DCM extracts IC 50 values, 15.1 and 12.6 1 µg/mL, respectively. From the spectroscopic analysis, the chemical components of the most active compounds toward tyrosinase was resolved as undecanoic acid. studies are ongoing to unravel the effect of this potent tyrosinase inhibitor in vivo to block the melanin synthesis in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin. concentration of out various cell experiments with different frequencies and concentrations, and the response surface is established to reﬂect the relationship among concentrations of MNPs, frequency of the applied magnetic ﬁeld, and thermal effect. The thermal effect of temperature rise was normalized for comparison. The response surface indicates that the higher concentrations of MNPs were in cells, the higher the thermal effect occurred. Moreover, the higher the frequency of the magnetic ﬁeld applied, the higher the thermal effect was that occurred.


Introduction
Lung tumor is one of the most common malignant tumors, which is the leading cause of cancer-related death worldwide. K-Ras mutations result in normal cells dividing uncontrollably; however, few treatments can effectively target these mutations as both chemotherapy and radiation are harmful to normal lung tissues. Finding a new way to treat K-Ras lung tumors more effectively and with fewer side effects is, therefore, urgent and important.
An increasing amount of research is being conducted in the role of magnetic nanoparticles (MNPs) in the inhibition of cell proliferation [1][2][3] because their unique characteristics offer attractive applications in the treatment of tumors [4]. For example, MNPs whose surface has been biochemically modified can be concentrated in a given area, thereby achieving targeted aggregation. Ramesh shows the binding of epidermal growth factor receptor (EGFR) antibodies to MNPs and how they inhibit the growth of tumor cells through cytotoxic mechanisms [5]. In their study, MNPs act as carriers, delivering drugs to the tumor location. A. Espinosa et al. investigate how iron oxide MNPs can be used to kill tumor cells by inducing hyperthermia, generated using high frequency (up to 100 kHz) magnetic fields, at the tumor location [6]. The hyperthermia comes from the tissue-field interactions. It could affect normal tissues adjacent to tumor cells. Consequently, researchers have investigated the non-thermal properties of MNPs for potential interference with tumor cells. When an alternating magnetic field is applied to a nanoparticle, a mechanical force can be induced by the magnetic field. Previous studies show that MNPs are affected by this applied field [7]. In 2016, Chen reported the use of spin-vortex, disk-shaped permalloy magnetic particles in a low-frequency, rotating magnetic field for the in vitro and in vivo destruction of glioma cells [8]. In 2019, Lunov studied the magneto-mechanical modulation of iron oxide nanoparticles for remote actuation of apoptosis in liver cancer cells, which enabled remote activation of apoptosis in liver cancer cells [9]. Although magneto-actuated cell apoptosis was proposed in a number of studies, the phenomenon of the MNPs cooperated with the magnetic field on A549 tumor cells and internal mechanisms of this approach have not been fully discovered.
In this study, Fe 3 O 4 @SiO 2 core-shell nanoparticles were cooperated with a magnetic field to intervene in the proliferation and migration of a K-Ras-driven A549 lung tumor cell line. Section 2 presents the preparation and characterizations of MNPs. Section 3 shows the design and simulation of the experimental platform. Section 4 provides analysis and calculations of MNPs in a magnetic field. The force calculation shows the magnetic force induced by MNPs with a magnetic field. Section 5 provides details of cell experiments. The experiments and analysis show that MNPs cooperated with a magnetic field could suppress not only the proliferation of tumor cells but also cell migration. Section 6 concludes the paper. This study suggests a new way to suppress the proliferation of K-Ras-driven lung tumors using MNPs cooperated with a magnetic field.

Preparation and Characterization of Magnetic Nanoparticles
Nanoparticles have been widely applied in bioengineering applications, such as imaging, molecular detection, and drug delivery. They are usually based on ferrous ferric oxide to ensure responsiveness to the applied magnetic field; however, magnetic ferrous ferric oxides have many disadvantages, e.g., agglomeration, oxidation, and biotoxicity. To overcome these deficiencies, magnetic ferrous ferric oxide nanoparticles are often coated with silica, after which they possess higher biocompatibility. Figure 1a shows the process flow of MNPs in this research.
The Fe 3 O 4 @SiO 2 core-shell nanoparticles in this study were manufactured as follows. First, 100 mL of 1 mol/L FeCl 3 ·6H 2 O solution and 50 mL of 1 mol/L FeCl 2 ·4H 2 O solution were mixed in a 250 mL three-neck flask. Next, a water bath was heated to 60 • C and 0.5 mol/L aqueous ammonia solution was added dropwise while stirring at 800 r/min. As the color of the mixture gradually changed from orange-red to black, ammonia was added to maintain a pH of about 11. The mixture was stirred for 30 min, ultrasonically dispersed for 30 min, and stirred again for 1 h. Tetraethyl orthosilicate (TEOS) was added to the ethanol deionized water ammonia solution for 24 h. Finally, the products were repeatedly washed with deionized water to ensure pH neutrality.
According to the procedure of the preparation, the MNPs were characterized by Transmission Electron Microscopy (TEM) and X-ray diffraction (XRD). Figure 1b shows the TEM of the prepared MNPs. The size diagram of the prepared MNPs has also been analyzed in Figure 1c. The mean diameter of the Fe3O4@SiO2 is 32.1 nm and the standard deviation is 5.1. The mean thickness of the SiO 2 shell is 7 nm. As can be seen from Figure 2a, the XRD patterns of the prepared Fe 3 O 4 @SiO 2 and the F e 3O 4 samples were analyzed. To evaluate the magnetic properties, the magnetic hysteresis loop of the MNPs was measured by LakeShore 7404 VSM in Figure 2b, and the saturation magnetization is 14.2 emu/g.   has been recognized frequently with its biological activity against pathogenic fungi, bacteria and different microbes [61,62]. However, this is the first report describing the affinity of this compound "Undecanoic acid" to an inhibitor of tyrosinase with comparable activity to kojic acid as commercial melanin biosynthesis inhibitors.

Conclusions
Tyrosinase productivity has been screened from various filamentous fungal isolates. Among these fungi, A. terreus and P. copticola were reported as the most potent tyrosinase producers. The overall yield of tyrosinase was maximized upon nutritional optimization bioprocess using the Plackett-Burman design. The enzyme activity from both fungal iso-lates were increased by about 7.5-folds with the response surface methodology optimiza-tion process over the control media. The enzyme was purified, and its various biochemical and catalytic properties were assessed, displaying a noticeable proximity to human tyro-sinase, emphasizing the usage of this enzyme as a model enzyme to study the mechanis-tics and kinetics of inhibition in response to various compounds. Targeting of the human tyrosinase is the main therapeutic approach to limit the over biogenesis of melanin and its hazardous derivatives, thus controlling the various pathogenetic disorders. The activ-ity of the purified tyrosinase was strongly inhibited by kojic acid and DCM extracts with IC50 values, 15.1 and 12.6 1 µg/mL, respectively. From the spectroscopic analysis, the chemical components of the most active compounds toward tyrosinase was resolved as undecanoic acid. Further studies are ongoing to unravel the effect of this potent tyrosinase inhibitor in vivo to block the melanin synthesis in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin.

Design and Simulations of The Cell Experiment Platform
The experiment platform comprised a signal generator, a stimulation coil, and a confocal microscope (LSM800, ZEISS, Germany). The frequency of the magnetic field could be adjusted by keyboard, and the intensity of the field could be changed by the potentiometer.

Optimization of the Stimulation Coil Integrated with the Confocal Microscope
To observe the growth of cells targeted by MNPs and the magnetic field, a CO 2 incubator was integrated with a confocal microscope. To provide a clear optical path and ensure that images could be taken effectively, a planar spiral coil with a central hole to allow the transmission of light was designed and realized.
As shown in Figure 3a, there are 80 turns printed on a printed circuit board that has a small volume and is easily manufactured. The merits of the proposed coil are not only suitable for the confocal microscope but also have a satisfactory heat dissipation condition to avoid overheat.
where μ0 is the space permeability, l Id  is the current element, r  is the vector at a 126 certain point, and r is the distance between l Id  and a certain point. 127 The Bz of a certain point is calculated using where R is the radius of the coil. The x, y, and z are the coordinates at a certain point. 129 The total magnetic field intensity of the point above the center of the planar coil can 130 be simplified as where N is the number of turns. According to Equation (3), the maximum magnetic 132 flux density at the targeted area is 2.0 mT by programming. As shown in Figure 3

136
The air around the stimulation coil was heated by the current passing through it. At 137 temperatures higher than 37.5 °C, cells can die through overheating. Therefore, it was nec-138 essary to analyze the temperature rise of the coil. 139 According to the heat equations [10], the temperature is calculated by The heat source in Equation (4) is, thus, determined by As shown in Figure 4, the compared stimulation coil is the type of solenoid. The pa-142 rameters of the two types of stimulation coil are shown in Table 1.

Simulation of the Magnetic Field
According to Biot-Savart's law, the magnetic flux density of the coil can be calculated as where µ 0 is the space permeability, Id l is the current element, r is the vector at a certain point, and r is the distance between Id l and a certain point. The B z of a certain point is calculated using where R is the radius of the coil. The x, y, and z are the coordinates at a certain point. The total magnetic field intensity of the point above the center of the planar coil can be simplified as where N is the number of turns. According to Equation (3), the maximum magnetic flux density at the targeted area is 2.0 mT by programming. As shown in Figure 3b, this result is close to the result of the FEM simulations. The magnetic flux density at the center of the culture dish was 1.8 mT.

Calculation of the Rise in Temperature of the Stimulation Coil
The air around the stimulation coil was heated by the current passing through it. At temperatures higher than 37.5 • C, cells can die through overheating. Therefore, it was necessary to analyze the temperature rise of the coil.
According to the heat equations [10], the temperature is calculated by The heat source in Equation (4) is, thus, determined by As shown in Figure 4, the compared stimulation coil is the type of solenoid. The parameters of the two types of stimulation coil are shown in Table 1.   Figure 5 shows the rise in temperature of the planar coils compared with a planar 147 solenoid coil. Based on the finite element method of calculation, the temperature at the 148 location of the culture dish was lower than that of the solenoid coil.   [11,12]. By exploiting cell endocytosis, MNPs can enter cells and exert magnetic forces on 157 the organelle [13]. Despite these advantages, the mechanism by which MNPs affect cell 158 growth is still unclear. The non-thermal effects of MNPs guided by magnetic forces can 159 be of key importance in the deployment of MNPs in a magnetic field against tumor cells.    Figure 5 shows the rise in temperature of the planar coils compared with a planar solenoid coil. Based on the finite element method of calculation, the temperature at the location of the culture dish was lower than that of the solenoid coil.   Figure 5 shows the rise in temperature of the planar coils solenoid coil. Based on the finite element method of calculation location of the culture dish was lower than that of the solenoid co

Analysis of the Magnetic Nanoparticles
When MNPs are placed in a magnetic field, they experienc phenomenon allows the manipulation of MNPs using an applied propriate parameters. In recent years, studies have explored the u netic field in the "wireless" manipulation of cell adhesion and [11,12]. By exploiting cell endocytosis, MNPs can enter cells and the organelle [13]. Despite these advantages, the mechanism by growth is still unclear. The non-thermal effects of MNPs guided

Analysis of the Magnetic Nanoparticles
When MNPs are placed in a magnetic field, they experience a magnetic force. The phenomenon allows the manipulation of MNPs using an applied magnetic field with appropriate parameters. In recent years, studies have explored the use of MNPs with a magnetic field in the "wireless" manipulation of cell adhesion and intercellular signaling [11,12]. By exploiting cell endocytosis, MNPs can enter cells and exert magnetic forces on the organelle [13]. Despite these advantages, the mechanism by which MNPs affect cell growth is still unclear. The non-thermal effects of MNPs guided by magnetic forces can be of key importance in the deployment of MNPs in a magnetic field against tumor cells. Therefore, analyzing and calculating the magnetic force and thermal field of MNPs is necessary.

Force Analysis of MNPs in a Magnetic Field
The magnetic field generated by a stimulation coil around MNPs can be considered a uniform field in calculations. Herein, a model of Helmholtz coil was established to generate a uniform magnetic field. The magnetic force can be calculated using the Maxwell stress tensor method. The Maxwell stress tensor is a second-rank tensor used in classical electromagnetism research to represent the interaction between electromagnetic forces and mechanical momentum [14].
Generally, the Maxwell stress tensor is an integral along with a closed area S around the target domain. As shown in Figure 6a, if the closed area S is located in a linear material, and the magnetic force can be obtained by Equation (6).
where S is the surface element, and n is a normal vector on closed area. has been recognized frequently with its biological activity against pathogenic fungi, bacteria and different microbes [61,62]. However, this is the first report describing the affinity of this compound "Undecanoic acid" to an inhibitor of tyrosinase with comparable activity to kojic acid as commercial melanin biosynthesis inhibitors.

Conclusions
Tyrosinase productivity has been screened from various filamentous fungal isolates. Among these fungi, A. terreus and P. copticola were reported as the most potent tyrosinase producers. The overall yield of tyrosinase was maximized upon nutritional optimization bioprocess using the Plackett-Burman design. The enzyme activity from both fungal iso-lates were increased by about 7.5-folds with the response surface methodology optimiza-tion process over the control media. The enzyme was purified, and its various biochemical and catalytic properties were assessed, displaying a noticeable proximity to human tyro-sinase, emphasizing the usage of this enzyme as a model enzyme to study the mechanis-tics and kinetics of inhibition in response to various compounds. Targeting of the human tyrosinase is the main therapeutic approach to limit the over biogenesis of melanin and its hazardous derivatives, thus controlling the various pathogenetic disorders. The activ-ity of the purified tyrosinase was strongly inhibited by kojic acid and DCM extracts with IC50 values, 15.1 and 12.6 1 According to the previous research, taking into account the medium viscosity (0.95cP), the magnetic force calculated based on the Maxwell stress tensor method is shown in Figure 6b. During the stimulation of the magnetic field, the maximum magnetic force of MNPs induced by the applied magnetic field is around 1.5 nN. According to a previous study [15], the magnetic force can influence cellular activity. The MNPs cooperated with a magnetic field can be considered as a "nano-motor," and can be used to interfere with the growth of the cells.

Thermal Analysis of MNPs in Magnetic Stimulations
Cell proliferation is sensitive to temperature. Due to the side effects of MNPs in high-frequency magnetic fields, temperature rises should be avoided.
In this section, the rise in temperature of MNPs is calculated using the bioheat equation [16]: where H is the actual temperature ( • C) and H a is the temperature of the environment (assumed as a constant 37 • C), k is the thermal conductivity of the culture medium, c is the specific heat, and w is the mass flow rate (kg/m 3 /s).
Based on the previous research, the frequency of the applied magnetic field for hyperthermia ranges from 100 kHz to 450 kHz, and for magnetic-actuated ranges from 0.01 kHz to 3 kHz. The concentration of MNPs inside the cells ranges from 5 pgFe/cell to 25 pgFe/cell [17][18][19]. As shown in Figure 7, the thermal effect in high and low-frequency conditions and at different MNPs concentrations is studied by numerical calculation (with the same specification of MNPs). To determine the appropriate frequencies and concentration of MNPs, we have carried out various cell experiments with different frequencies and concentrations, and the response surface is established to reflect the relationship among concentrations of MNPs, frequency of the applied magnetic field, and thermal effect. The thermal effect of temperature rise was normalized for comparison. The response surface indicates that the higher concentrations of MNPs were in cells, the higher the thermal effect occurred. Moreover, the higher the frequency of the magnetic field applied, the higher the thermal effect was that occurred. Thus, based on numerical calculation and cell experiments, the concentr MNPs in the culture medium is specified as 5 μg/ml (corresponding to 16.51 pg and the frequency of the applied magnetic field is 2 kHz.  Thus, based on numerical calculation and cell experiments, the concentration of MNPs in the culture medium is specified as 5 µg/mL (corresponding to 16.51 pgFe/cell) and the frequency of the applied magnetic field is 2 kHz.

Preparation of A549 Lung Tumor Cells
A549 tumor cells are adenocarcinomic human alveolar basal epithelial cells. These cells have played a key role in the development of lung tumor treatment. In this study, K-Ras mutated A549 tumor cells were obtained from the American Tissue Culture Collection. Considering the duration of the stimulation, the cells were carried on the passage with a ratio of 1:3. The fourth generation cells were used for the cell experiment.
According to the preparation of the MNPs, the particles were prepared and sterilized by ultraviolet (UV) irradiation, suspended in phosphate-buffered saline (PBS) and Roswell Park Memorial Institute (RPMI, Sigma Life Science, St. Louis, MO, USA) 1640 medium containing 10% fetal bovine serum (FBS, Gibco by Life Technologies, Carlsbad, CA, USA). Before the cell experiments, the A549 cells were cultured and maintained in RPMI 1640 and supplemented with 10% fetal bovine serum (FBS). A549 cells were cultured in 75 cm 2 flasks with 20 mL RPMI solutions. They were collected by digesting with trypsin EDTA from the flask. After digestion, the A549 tumor cells were transferred into a 35 mm culture dish with the MNPs-RPMI medium.
To investigate the location of the MNPs, the A549 cells were labeled by RFP-GFP-LC3 lentiviral transfection. As shown in Figure 8a, the nucleus is stained orange and the cytoplasm is stained green. According to the TEM image and light microscope image, the MNPs were endocytosed by A549 cells. In Figure 8b, the dark area shows that MNPs are endocytosed by tumor cells and aggregated inside the cells. As shown in Figure 8c, the A549 cell is in apoptosis, stimulated by MNPs cooperated with a magnetic field.
has been recognized frequently with its biological activity against pathogenic fungi, bacteria and different microbes [61,62]. However, this is the first report describing the affinity of this compound "Undecanoic acid" to an inhibitor of tyrosinase with comparable activity to kojic acid as commercial melanin biosynthesis inhibitors.

Conclusions
Tyrosinase productivity has been screened from various filamentous fungal isolates. Among these fungi, A. terreus and P. copticola were reported as the most potent tyrosinase producers. The overall yield of tyrosinase was maximized upon nutritional optimization bioprocess using the Plackett-Burman design. The enzyme activity from both fungal iso-lates were increased by about 7.5-folds with the response surface methodology optimiza-tion process over the control media. The enzyme was purified, and its various biochemical and catalytic properties were assessed, displaying a noticeable proximity to human tyro-sinase, emphasizing the usage of this enzyme as a model enzyme to study the mechanis-tics and kinetics of inhibition in response to various compounds. Targeting of the human tyrosinase is the main therapeutic approach to limit the over biogenesis of melanin and its hazardous derivatives, thus controlling the various pathogenetic disorders. The activ-ity of the purified tyrosinase was strongly inhibited by kojic acid and DCM extracts with IC50 values, 15.1 and 12.6 1 µg/mL, respectively. From the spectroscopic analysis, the chemical components of the most active compounds toward tyrosinase was resolved as undecanoic acid. Further studies are ongoing to unravel the effect of this potent tyrosinase inhibitor in vivo to block the melanin synthesis in fungi and human, that could be a novel drug to various diseases associated with hyperpigmentation by melanin.

Cell Proliferation
A549 cells were divided into four groups: Group A, Group B, Group C, and Group D. Cells in Group A were stimulated using a magnetic field, those in Group B were stimulated using a magnetic field cooperated with MNPs, those in Group C were the control group, and those in Group D were cultured with MNPs without the magnetic field. Our previous study showed that a magnetic field can suppress the growth of A549 cells [20,21]. To study the non-thermal effects of MNPs with a magnetic field, the cells were counted by an automated cell counter (TC10, BIO-RAD, Hercules, CA, USA) every 24 h and captured every 12 h. As shown in Figure 9, after 72 h, the ratio of cell proliferation in Group A has been suppressed by the magnetic field. Also, the ratio of cell proliferation in Group B is 14.7% lower than that in Group A. The group with only MNPs has no significant effect on cell proliferation. To ensure verifiable repeatability, the cell experiment was repeated three times.

Cell Proliferation
A549 cells were divided into four groups: Group A, Group B D. Cells in Group A were stimulated using a magnetic field, those ulated using a magnetic field cooperated with MNPs, those in Gro group, and those in Group D were cultured with MNPs without t previous study showed that a magnetic field can suppress the grow To study the non-thermal effects of MNPs with a magnetic field, by an automated cell counter (TC10, BIO-RAD, USA) every 24 hou 12 hours. As shown in Figure 9, after 72 hours, the ratio of cell pr has been suppressed by the magnetic field. Also, the ratio of cell pr is 14.7% lower than that in Group A. The group with only MNPs h on cell proliferation. To ensure verifiable repeatability, the cell exp three times.

Scratch Wound-Healing Assay
To discover the inhibitory effect on the migration of tumor healing assay was conducted. The scratch wound healing assay ha

Scratch Wound-Healing Assay
To discover the inhibitory effect on the migration of tumor cells, a scratch wound healing assay was conducted. The scratch wound healing assay has been widely adopted and modified to study the effects of a variety of experimental conditions. In a typical wound healing assay, it is specifically a 2D cell migration approach to semi-quantitatively measure cell migration of a sheet of cells. This scratch can be made through various approaches, such as mechanical, thermal, or chemical damage. The purpose of this scratch is to produce a cell-free area in hopes of inducing cells to migrate and close the gap [22].
As can be seen from Figure 10, by cell counting, the level of cell migration in Group B is 18.7% lower than that in Group A. This experimental result shows that MNPs cooperated with a magnetic field could suppress the migration of A549 tumor cells.
. Sci. 2021, 11, x FOR PEER REVIEW As can be seen from Figure 10, by cell counting, the level of cell m 18.7% lower than that in Group A. This experimental result show ated with a magnetic field could suppress the migration of A549 Figure 10. The cell counting of the wound-healing assay.

Conclusions
In this paper, MNPs for use in treating tumors were prepare magnetic force and thermal analysis of MNPs in a magnetic field The simulations show that the experimental platform can g netic field while avoiding any notable temperature rises and th force can affect cellular activities. The cell experiments illustrate tion of A549 cells could be suppressed and the level of cell migra relationship between the surface modification of MNPs and thei effects on tumor cells will be investigated in future studies. Thi suppress the growth of tumor cells but can also strengthen the i netic field stimulation on lung tumors caused by K-Ras mutation

Conclusions
In this paper, MNPs for use in treating tumors were prepared and characterized. The magnetic force and thermal analysis of MNPs in a magnetic field were studied.
The simulations show that the experimental platform can generate the desired magnetic field while avoiding any notable temperature rises and that the induced magnetic force can affect cellular activities. The cell experiments illustrate that the rate of proliferation of A549 cells could be suppressed and the level of cell migration can be reduced. The relationship between the surface modification of MNPs and their non-thermal inhibitory effects on tumor cells will be investigated in future studies. This approach can not only suppress the growth of tumor cells but can also strengthen the inhibitory effects of magnetic field stimulation on lung tumors caused by K-Ras mutations.