A Novel Digital Technique to Quantify the Area and Volume of Enamel Removal after Interproximal Enamel Reduction

: The aim of this study was to show a novel and accurate digital measurement protocol by analyzing the area and volume for interproximal tooth enamel surface reduction. In total, 14 lower teeth from all dental sectors were embedded into an epoxy resin and distributed as the lower dental arch, keeping the contact points. The experimental model was submitted to an intraoral digital impression before and after interproximal tooth enamel surface reduction using air-rotor strips and then re-contouring and polishing the interproximal enamel surfaces. These steps helped obtain standard tessellation language (STL) digital ﬁles. Furthermore, each tooth in the preoperative and postoperative full-arch STL digital ﬁles was segmented individually and aligned to analyze the area and volume of the interproximal tooth enamel surface reduction using engineering morphometry software. Descriptive analysis of the area and volume of the interproximal tooth enamel surface reduction was performed using a Student t-test. Higher enamel reduction area (3.53 ± 3.08 mm 2 ) and volume (0.32 ± 0.22 mm 3 ) values were shown on the distal surface compared with the area (2.97 ± 3.05 mm 2 ) and volume (0.22 ± 0.16 mm 3 ) of the enamel reduction on the mesial surface measured using the morphometric measurement digital protocol. The morphometric measurement protocol is an accurate digital measurement protocol for analyzing the area and volume of interproximal enamel surface reduction.


Introduction
Interproximal enamel reduction (IER) or stripping is considered an irreversible technique used to remove controlled amounts of interproximal enamel surfaces to achieve a reduction in the mesiodistal length of interproximal dental surfaces. The result is an increase in the available space in the dental arch [1,2]. IER is considered a more conservative orthodontic therapy than dental extractions [3] and, therefore, it is used to increase the available space without tooth extractions, allowing for teeth alignment and correct labioversion of the incisors [4] with a mild or moderate bone-dental discrepancy (BDD) between 4-8 mm [5,6]. IER is also recommended to improve the unaesthetic appearance caused by the loss of the interdental papilla, which is a consequence of bone loss or occlusal interproximal tooth contact [7]. In addition, IER is also used in temporary dentition to facilitate the eruption of partially erupted teeth blocked by insufficient space [8]. However, IER procedures are not recommended in small teeth, hypoplasia, severe tooth rotations, high risk of caries, poor oral hygiene, and young patients with large pulp chambers [9]. In addition, mechanic and manual IER techniques have been reported [10,11] to affect the interdental tooth enamel surfaces, leaving grooves and scratches [12]. For this reason, final polishing of interproximal tooth enamel surfaces after IER procedures are mandatory to reduce the number of dental abrasions and to obtain an interproximal tooth surface similar to untreated interproximal tooth enamel surfaces [5], ensuring favorable long-term prognosis [13]. Many measurement procedures have been used to analyze interproximal tooth enamel surfaces after IER techniques; however, most studies have used microscopic measurement methods [14] and profilometry [15], which are useful for analyzing the microroughness on interproximal tooth enamel surfaces after interproximal tooth enamel surface removal. Nonetheless, it is impossible to apply this in clinical situations because it is necessary to extract the teeth. De Felipe et al. and Johner et al. reported a digital technique to analyze the total amount of stripping in order to calculate the maximum mesiodistal distance in teeth based on the position of the tooth's axis; however, the placement of this axis was not tested, and they measure neither the area nor the volume of the IER procedure [16,17]. However, the main novelty of the morphometric measurement technique is the possibility of using it in vivo in a clinical situation, wherein the acquisition of the digital information is done by intraoral scanners. The development of digital measurement procedures in dental research has increased the accuracy, simplicity, and versatility of acquiring information in research applications [18]. Intraoral scanners communicate easily with computer-aided design/computer-aided manufacturing (CAD/CAM) systems. This technology has also been introduced in orthodontics, thus allowing a completely digital workflow, from impression to final framework, with clinical reliability [19] and good patients' feedback [20]. Different image capture systems-i.e., triangulation, light stripe projection, confocal parallelism images, and stereophotogrammetric videos-have been used in dental research [21]. Regardless of the scanning method, the measurements led to a three-dimensional capture made up of a point cloud that represents the object and its reference in space [22]. The most widely used digital format is standard tessellation language (STL). This format describes a succession of triangulated surfaces, where each triangle is defined by three points on a surface, which allows for a high accuracy measurement [23]. This triangulation converts cloud points into the mesh in order to calculate the volume and area of different treatments carried out on the tooth surface.
The aim of this study was to show a novel and accurate digital measurement protocol in order to analyze the area and volume of interproximal tooth enamel surface reduction.

Study Design
In total, 14 lower teeth from all dental sectors, extracted for periodontal or orthodontic reasons, without caries, restorations, or fractures, were selected in this study at the Alfonso X El Sabio University (Madrid, Spain), Master's Degree program in Orthodontics at the University of Salamanca (Salamanca, Spain) and the Department of Stomatology at the University of Valencia (Valencia, Spain), between September and October 2020. An in vitro study was conducted in accordance with the principles defined in the German Ethics Committee s statement for the use of organic tissues in medical research (Zentrale Ethikkommission, 2003), and was authorized by the Ethical Committee of the Faculty of Health Sciences, University Alfonso X el Sabio (Madrid, Spain) in September 2020 (Process No. 09/2020). All patients gave their informed consent to transfer the teeth.

Experimental Procedure
The teeth were embedded into an experimental model of epoxy resin (ref. : 20-8130-128. EpoxiCure ® , Buehler, IL, USA), keeping the contact points. Afterward, the experimental model with the embedded teeth was submitted to a baseline intraoral scan (True Definition,  Figure 1a) to generate an STL digital file using a cloud of points that create a tessella network, representing 3-dimensional objects as polygons composed of equilateral triangle tessellas [24,25]. The capturing images procedure was performed following manufacture recommendations by scanning the incisal/occlusal plane and the vestibular and lingual surfaces. After that, an interproximal enamel reduction of 0.2 mm at both interproximal surfaces for each tooth was performed using air-rotor strips (Ref.: 060B, Ortho-Strip system, Intensiv Dental, Montagnola, Switzerland) according to the manufacturers' recommendations. We then re-contoured to produce an adequate interproximal shape and to polish the surfaces (Softflex system, 3M ESPE ™, Saint Paul, MN, USA) and the Komet ASR-Set (Komet Medical, Lemgo, Germany). Then, the experimental model was submitted to a postoperative interproximal enamel reduction intraoral scan (True Definition, 3M ESPE ™, Saint Paul, MN, USA) (STL2) (Figure 1b).

Experimental Procedure
The teeth were embedded into an experimental model of epoxy resin (ref. : 20-8130-128. EpoxiCure ® , Buehler, IL, USA), keeping the contact points. Afterward, the experimental model with the embedded teeth was submitted to a baseline intraoral scan (True Definition, 3M ESPE ™, Saint Paul, MN, USA) (STL1) via a 3D in-motion video imaging technology ( Figure 1a) to generate an STL digital file using a cloud of points that create a tessella network, representing 3-dimensional objects as polygons composed of equilateral triangle tessellas [24,25]. The capturing images procedure was performed following manufacture recommendations by scanning the incisal/occlusal plane and the vestibular and lingual surfaces. After that, an interproximal enamel reduction of 0.2 mm at both interproximal surfaces for each tooth was performed using air-rotor strips (Ref.: 060B, Ortho-Strip system, Intensiv Dental, Montagnola, Switzerland) according to the manufacturers' recommendations. We then re-contoured to produce an adequate interproximal shape and to polish the surfaces (Softflex system, 3M ESPE ™, Saint Paul, MN, USA) and the Komet ASR-Set (Komet Medical, Lemgo, Germany). Then, the experimental model was submitted to a postoperative interproximal enamel reduction intraoral scan (True Definition, 3M ESPE ™, Saint Paul, MN, USA) (STL2) (Figure 1b).     The teeth were embedded into an experimental model of epoxy resin (ref. : 20 128. EpoxiCure ® , Buehler, IL, USA), keeping the contact points. Afterward, the e mental model with the embedded teeth was submitted to a baseline intraoral scan Definition, 3M ESPE ™, Saint Paul, MN, USA) (STL1) via a 3D in-motion video im technology ( Figure 1a) to generate an STL digital file using a cloud of points that cr tessella network, representing 3-dimensional objects as polygons composed of equi triangle tessellas [24,25]. The capturing images procedure was performed following ufacture recommendations by scanning the incisal/occlusal plane and the vestibula lingual surfaces. After that, an interproximal enamel reduction of 0.2 mm at both proximal surfaces for each tooth was performed using air-rotor strips (Ref.: 060B, O Strip system, Intensiv Dental, Montagnola, Switzerland) according to the manufact recommendations. We then re-contoured to produce an adequate interproximal and to polish the surfaces (Softflex system, 3M ESPE ™, Saint Paul, MN, USA) an Komet ASR-Set (Komet Medical, Lemgo, Germany). Then, the experimental mode submitted to a postoperative interproximal enamel reduction intraoral scan (True D tion, 3M ESPE ™, Saint Paul, MN, USA) (STL2) (Figure 1b).

Alignment Procedure
Afterward, the teeth from 3.7 to 4.   Then, the individually segmented teeth of the preoperative ( Figure 3a) and postoperative ( Figure 3b) interproximal enamel reduction STL digital files were superimposed by using the non-varied buccal, occlusal, and lingual surfaces of the teeth as a reference with the best-fit algorithm.
Then, the individually segmented teeth of the preoperative ( Figure 3a) and postoperative ( Figure 3b) interproximal enamel reduction STL digital files were superimposed by using the non-varied buccal, occlusal, and lingual surfaces of the teeth as a reference with the best-fit algorithm.

Measurement Procedure
After the alignment procedure between the individually segmented teeth in the preoperative and postoperative interproximal enamel reduction STL digital files, the area of enamel removed on the interproximal surfaces was measured by comparing preoperative boundaries ( Figure 4a) and postoperative interproximal enamel reduction STL digital files ( Figure 4b). The distal surfaces of teeth 3.7 and 4.7 were not submitted to the interproximal enamel reduction procedure.

Measurement Procedure
After the alignment procedure between the individually segmented teeth in the preoperative and postoperative interproximal enamel reduction STL digital files, the area of enamel removed on the interproximal surfaces was measured by comparing preoperative boundaries ( Figure 4a) and postoperative interproximal enamel reduction STL digital files ( Figure 4b). The distal surfaces of teeth 3.7 and 4.7 were not submitted to the interproximal enamel reduction procedure. erative ( Figure 3b) interproximal enamel reduction STL digital files were sup by using the non-varied buccal, occlusal, and lingual surfaces of the teeth as with the best-fit algorithm.

Measurement Procedure
After the alignment procedure between the individually segmented teeth operative and postoperative interproximal enamel reduction STL digital files, enamel removed on the interproximal surfaces was measured by comparing pr boundaries ( Figure 4a) and postoperative interproximal enamel reduction STL ( Figure 4b). The distal surfaces of teeth 3.7 and 4.7 were not submitted to the int enamel reduction procedure.  In addition, the volume of enamel removed after the interproximal enamel reduction procedure was selected and isolated in both preoperative ( Figure 5a) and postoperative interproximal enamel reduction STL digital files (Figure 5b) via reverse selection. The selected area's normal tessella network for the enamel removed in the preoperative ( Figure  5a) and postoperative interproximal enamel reduction STL digital files (Figure 5b) were flipped to obtain a closed polygon with the selected area of the enamel removed to obtain the volume of the enamel removed after the interproximal enamel reduction procedures. Afterward, the point of contact between the mesial and distal interproximal enamel reduction meshes (Figure 6a) were aligned perpendicular to the "X" axis ( Figure 6b). Finally, 10 points were positioned at the inner surface of the interproximal enamel reduction mesh of the anterior tooth (Figure 7a). Then, 10 lines parallel to the "X" axis were projected (Figure 7b) from the previous points to the inner surface of the interproximal enamel reduction mesh of the posterior tooth (Figure 7c). Then, 10 new points were created at the intersection of the lines and the inner surface of the interproximal enamel reduction mesh of the posterior tooth, creating 10 pairs of points (Figure 7d). The distance of each pair of points was measured. These distances were used in order to obtain a mean value of the interproximal enamel reduction between adjacent teeth. Afterward, the point of contact between the mesial and distal interproximal enamel reduction meshes (Figure 6a) were aligned perpendicular to the "X" axis ( Figure 6b).
In addition, the volume of enamel removed after the interproximal enamel reduction procedure was selected and isolated in both preoperative (Figure 5a) and postoperative interproximal enamel reduction STL digital files (Figure 5b) via reverse selection. The selected area's normal tessella network for the enamel removed in the preoperative ( Figure  5a) and postoperative interproximal enamel reduction STL digital files (Figure 5b) were flipped to obtain a closed polygon with the selected area of the enamel removed to obtain the volume of the enamel removed after the interproximal enamel reduction procedures. Afterward, the point of contact between the mesial and distal interproximal enamel reduction meshes (Figure 6a) were aligned perpendicular to the "X" axis ( Figure 6b). Finally, 10 points were positioned at the inner surface of the interproximal enamel reduction mesh of the anterior tooth (Figure 7a). Then, 10 lines parallel to the "X" axis were projected (Figure 7b) from the previous points to the inner surface of the interproximal enamel reduction mesh of the posterior tooth (Figure 7c). Then, 10 new points were created at the intersection of the lines and the inner surface of the interproximal enamel reduction mesh of the posterior tooth, creating 10 pairs of points (Figure 7d). The distance of each pair of points was measured. These distances were used in order to obtain a mean value of the interproximal enamel reduction between adjacent teeth.

Statistical Analysis
Statistical analysis of the measurement variables was carried out using SAS 9.4 (SAS Institute Inc., Cary, NC, USA). Descriptive statistics of the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of each tooth were expressed as the mean and standard deviation (SD). Additionally, also the minimum, median, and maximum values were calculated for each variable. A comparative analysis was performed by comparing the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files using the Student's t-test; the variables had a normal distribution. The normality of the variables was analyzed using the Shapiro-Wilk normality test. The statistical significance was set at p ˂0.05.

Results
The means and SD values for the area of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files are displayed in Table 1 and Figure 8.

Statistical Analysis
Statistical analysis of the measurement variables was carried out using SAS 9.4 (SAS Institute Inc., Cary, NC, USA). Descriptive statistics of the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of each tooth were expressed as the mean and standard deviation (SD). Additionally, also the minimum, median, and maximum values were calculated for each variable. A comparative analysis was performed by comparing the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files using the Student's t-test; the variables had a normal distribution. The normality of the variables was analyzed using the Shapiro-Wilk normality test. The statistical significance was set at p < 0.05.

Results
The means and SD values for the area of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files are displayed in Table 1 and Figure 8.   The distal surface of the teeth, which underwent the interproximal enamel reduction procedure, showed a high enamel reduction area (3.53 ± 3.08 mm 2 ) compared to the enamel reduction area of the mesial surface of the teeth (2.97 ± 3.05 mm 2 ). The paired ttest did not show statistically significant differences between the mesial and distal areas of the removed enamel (p = 0.529).
The mean and SD values for the volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files are displayed in Table 2 and Figure 9. Table 2. Descriptive analysis of the volume (mm 3 ) of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files. The distal surface of the teeth, which underwent the interproximal enamel reduction procedure, showed a high enamel reduction area (3.53 ± 3.08 mm 2 ) compared to the enamel reduction area of the mesial surface of the teeth (2.97 ± 3.05 mm 2 ). The paired t-test did not show statistically significant differences between the mesial and distal areas of the removed enamel (p = 0.529).
The mean and SD values for the volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files are displayed in Table 2 and Figure 9. Table 2. Descriptive analysis of the volume (mm 3 ) of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files. The distal surface of the teeth submitted to the interproximal enamel reduction procedure showed a high enamel reduction volume (0.32 ± 0.22 mm 3 ) compared to the enamel reduction volume of the teeth submitted to the mesial surface in the interproximal enamel reduction procedure (0.22 ± 0.16 mm 3 ). The paired t-test did not show statistically significant differences between the mesial and distal volumes of the removed enamel (p = 0.430). Sci. 2021, 11, x FOR PEER REVIEW 8 of 11 Figure 9. Box plot of the volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces of the STL digital files. "*" represent outliers in line 8.

Surface
The distal surface of the teeth submitted to the interproximal enamel reduction procedure showed a high enamel reduction volume (0.32 ± 0.22 mm 3 ) compared to the enamel reduction volume of the teeth submitted to the mesial surface in the interproximal enamel reduction procedure (0.22 ± 0.16 mm 3 ). The paired t-test did not show statistically significant differences between the mesial and distal volumes of the removed enamel (p = 0.430).

Discussion
The results obtained in the present study showed that the morphometric measurement protocol allowed for an accurate measurement technique to analyze the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces.
Profilometry, rugosimetry, scanning electron microscopy (SEM), and atomic force microscopy (AFM) measurement techniques have been used in order to assess the enamel surface roughness [17,18]. The SEM measurement technique provides two-dimensional (2D) analysis about the qualitative and morphological changes of the enamel surface [26]; however, profilometry and AFM measurement techniques report a three-dimensional (3D) analysis of the enamel surface roughness for subsequent evaluation [23]. In addition, profilometry and rugosimetry provide a micro-scale 3D image [27,28], whereas AFM provides a nanoscale 3D image with vertical and lateral resolution [29]. García Vargas et al. analyzed the surface roughness of different interproximal enamel reduction procedures using rugosimetry and obtained a higher roughness (Ra) value using strip bur (0.54 µm), as well as strip bands (0.41 µm) and strip discs (0.34 µm). In addition, interproximal enamel reduction performed with strip burs revealed the highest Vickers (vH) microhardness values (365 vH), followed by strip discs (327 vH) and strips bands (317 vH) [30]. Kilinc et al. reported that the surface roughness performed with metal strip bands and the air-rotor stripping procedure showed statistically significant differences via SEM evaluation. However, 70% less surface roughness was observed in teeth submitted to air-rotor stripping and 30% in the teeth submitted to strip bands after the first month [31]. Kaaouara et al. analyzed the interproximal tooth enamel surface using SEM and reported that singleside diamond strip bands showed a mean enamel roughness of 15 µm and ContactEZ diamond files, which also showed a mean enamel roughness of 10 µm [4]. Meredith et al. analyzed the enamel nanotopography after interproximal enamel reduction using atomic force microscopy (AFM) and reported that the enamel surface becomes progressively smoother from burs to strips and discs to polishers. In addition, surface roughness was higher using a medium roughness strip bur (707 nm), and decreased using medium strip

Discussion
The results obtained in the present study showed that the morphometric measurement protocol allowed for an accurate measurement technique to analyze the area and volume of the enamel removed after the interproximal enamel reduction procedure at the mesial and distal interproximal enamel surfaces.
Profilometry, rugosimetry, scanning electron microscopy (SEM), and atomic force microscopy (AFM) measurement techniques have been used in order to assess the enamel surface roughness [17,18]. The SEM measurement technique provides two-dimensional (2D) analysis about the qualitative and morphological changes of the enamel surface [26]; however, profilometry and AFM measurement techniques report a three-dimensional (3D) analysis of the enamel surface roughness for subsequent evaluation [23]. In addition, profilometry and rugosimetry provide a micro-scale 3D image [27,28], whereas AFM provides a nanoscale 3D image with vertical and lateral resolution [29]. García Vargas et al. analyzed the surface roughness of different interproximal enamel reduction procedures using rugosimetry and obtained a higher roughness (Ra) value using strip bur (0.54 µm), as well as strip bands (0.41 µm) and strip discs (0.34 µm). In addition, interproximal enamel reduction performed with strip burs revealed the highest Vickers (vH) microhardness values (365 vH), followed by strip discs (327 vH) and strips bands (317 vH) [30]. Kilinc et al. reported that the surface roughness performed with metal strip bands and the airrotor stripping procedure showed statistically significant differences via SEM evaluation. However, 70% less surface roughness was observed in teeth submitted to air-rotor stripping and 30% in the teeth submitted to strip bands after the first month [31]. Kaaouara et al. analyzed the interproximal tooth enamel surface using SEM and reported that single-side diamond strip bands showed a mean enamel roughness of 15 µm and ContactEZ diamond files, which also showed a mean enamel roughness of 10 µm [4]. Meredith et al. analyzed the enamel nanotopography after interproximal enamel reduction using atomic force microscopy (AFM) and reported that the enamel surface becomes progressively smoother from burs to strips and discs to polishers. In addition, surface roughness was higher using a medium roughness strip bur (707 nm), and decreased using medium strip bands (501 nm), fine strip burs (407 nm), fine strip bands (318 nm), mesh strip discs (307 nm), curved strip discs (224 nm), and a Sof-Lex polishing device (37 nm) [32]. Nevertheless, the previously described measurement techniques do not have the capability to accurately measure the area and volume of the interproximal enamel reduction because these measurement techniques perform a qualitative observational analysis. The morphometric measurement technique allows for an accurate, repeatable, and reproducible analysis that looks at the amount of cement remaining after a fixed multibracket appliance removal, the amount of orthodontic cement necessary to fix multibracket appliances, and the amount of enamel removed after fixed multibracket appliance removal, even in a clinical setting that prevents teeth extraction [33].
In accordance with this study, it is possible to accurately measure the area and volume of interproximal enamel surfaces (i.e., mesial and distal), independent of the width of each tooth, which is in contrast with previously published studies [19,20]. However, some factors have been highlighted to affect the accuracy of the digital scanner and hence on the quality of the STL digital files increasing the inaccuracy of the alignment procedure. Moreover, the selection of the digital scanner is highly relevant to provide a specific number of equilateral triangles of the tesella network, which can influence the accuracy of the measurement procedure.
The accuracy of the STL digital files through intraoral and extraoral scan devices depends on multiple factors related to the scanning process, such as the presence of saliva, adjacent structures, teeth, light reflection, presence of blood, type of material used during scanning, and the time of the scanning process [22,34], even though intraoral scan devices have reported smaller deviation values compared to extraoral scan devices [35]. Flügge et al. reported the influence of angle and distance of intraoral scan devices on the accuracy of the obtained STL digital files [36]; however, the accuracy of the obtained STL digital files has not been related to the distance of the extraoral scan device to the scanned object. Sason et al. reported that intraoral scan devices provide more accuracy compared to extraoral scanners [34]. Moreover, Ender and Mehl analyzed the accuracy of full-arch digital impressions using different intraoral scan devices and reported that the CEREC Bluecam scan showed a mean deviation of 80 µm in the posterior areas of the dental arch, whereas iTero showed a mean deviation of 120 µm and CEREC Omnicam showed a mean deviation of 130µm. Nevertheless, no statistically significant differences were observed between the intraoral scan devices (p > 0.05) [37]. In addition, Ender et al. reported no statistically significant differences (p > 0.05) between the accuracy of CEREC Bluecam, CEREC Omnicam, iTero, Lava COS, Lava True Definition, TRIOS, and TRIOS color digital for full-arch digital impressions [38]. However, Kuhr et al. analyzed the trueness of full-arch digital impressions between three digital scanners and reported statistically significant differences (p < 0.05) between the trueness values of the Lava True Definition and CEREC Onmicam; however, no statistically significant differences (p > 0.05) were shown between the Lava True Definition and TRIOS. The standard deviation about the distance measurement was 23 µm for 3M True Definition, 37 µm for Heraeus Cara TRIOS, and 214 µm for CEREC Omnicam. In addition, the angle measurement was for 0.06 • , 0.13 • , and 0.28 • for True Definition, Heraerus Cara TRIOS, and CEREC Omnicam, respectively [39]. Moreover, Guth et al. reported higher mean trueness of True Definition (21.8 µm) than CEREC Bluecam (34.2 µm), CEREC Omnicam (43.4 µm), iTero (49.0 µm), Lava COS (47.7 µm), TRIOS (25.7 µm), and TRIOS color (26.1 µm) for partial lower arch scanning [40]. Moreover, power-dependent digital impression systems provide more accurate STL digital files than no-powder dependent digital impression systems because light refraction and scattering is prevented by the use of powder, which also allows for an accurate determination of object depth [41,42]. Currently, there is no acquisition data system through digital scan devices that can be considered more accurate than others due to the lack of standardized protocols and randomized clinical trials [22]; however, Kulczyk et al. demonstrated that 3D models derived from optical scanners are 10 times more detailed in the number of tessellas in the network describing the tooth surface and 4-5 times more accurate in terms of standard deviation compared with experimental models obtained with CBCT with a standard resolution [43].

Conclusions
In conclusion, the morphometric measurement protocol is an accurate digital measurement protocol for analyzing the area and volume of interproximal enamel surface reduction. However, clinical trials are necessary to ensure the potential accuracy of this novel measurement procedure.