The Role of Wetland Plants on Wastewater Treatment and Electricity Generation in Constructed Wetland Coupled with Microbial Fuel Cell

: CWMFC is a novel technology that has been used for almost a decade for concurrent wastewater treatment and electricity generation in varying scopes of domestic, municipal, and industrial applications since its implementation in 2012. Its advantage of low-cost enhanced wastewater treatment and sustainable bioelectricity generation has gained considerable attention. Nevertheless, the overall efﬁciency of this novel technology is inclined by several operating factors and conﬁguration strands, such as pH, sewage composition, organic loading, electrode material, ﬁlter media, electrogens, hydraulic retention time, and macrophytes. Here, we investigate the effect of the wetland plant component on the overall performance of CWMFCs. The macrophyte’s involvement in the oxygen input, nutrient uptake, and direct degradation of pollutants for the required treatment effect and bioelectricity production are discussed in more detail. The review identiﬁes and compares planted and unplanted CWMFC with their efﬁciency on COD removal and electricity generation based on previous and recent studies.


Introduction
Over the decades, many wastewater treatment technologies have been employed to address the wastewater environmental menace. Wastewater treatment technologies, which consist of trickling filters, activated sludge, reverse osmosis, and membrane filters, are currently being used to treat all types of organic and toxic wastewater from industrial and municipal sources. However, they are not very productive, with regards to the cost and energy demand required in their operation [1]. It is projected that USD 2 trillion will be required in the U.S.A over the next 20 years to construct, operate, and maintain wastewater and drinking water facilities [2]. In addition to the current annual costs of USD 25 billion, around USD 45 billion is expected for wastewater infrastructure upgrades, with over half of operating expenditures aimed at aeration of wastewater. Power production measured here only for aeration could provide much-needed energy in the U.S.A from industrial wastewater alone [3]. According to Gude (2015), some of these conventional wastewater treatment systems require 0.3-0.6 kW·h·m −3 for treatment, whereas inherent in the same their subsequent effects on CW-MFC bioenergy production. This study will help investigate the potential of the technology with or without the use of wetland plants and guide future research in selecting appropriate wetland plants.

Configuration of CWMFC
CWMFC is a hybrid system that seeks to integrate MFCs into built wetlands by leveraging the triple interaction of substrates, the plant and microorganisms' physical, chemical, and biotic elements for wastewater treatment and electricity production [25][26][27]. This hybrid system syndicates the advantage of two systems in a way that is proficient in attaining high levels of wastewater reuse and bioenergy [26]. On the basis of researchers identifying a variation of dissolved oxygen (DO) along the vertical profile of CW's natural environment, this integration was conceived to be plausible, which creates a naturally prevailing stratified redox gradient similar to that of MFC in its two-chambered cell, as shown in Figure 1 [16,28,29]. A typical CWMFC is, therefore, built similar to a traditional MFC with two major compartments-the lower anaerobic chamber and the upper aerobic chamber [27][28][29][30][31]with an anode electrode deeply buried within the anaerobic chamber and cathode electrode positioned at the air-water interface around the root zone of the plant, also called the aerobic or cathodic section [23]. These electrodes are then connected using titanium wires with an applied external resistance crucial for electricity generation. By positioning the anode at the lower anaerobic zone and inserting the cathode at the air-water interface, CW-MFC takes good advantage of the existing redox condition [31]. The optimization of this redox gradient or potential difference established betwixt the anode and the cathode is pivotal for power generation and contaminant removal in CWMFC [32]. Since the onset of this hybrid technology, carbon, and graphite materials have been widely used as electrodes, owing to their suitable surface microbial attachment and growth, high electrical conductivity, non-oxidative nature, and riveting characteristics for biofilm formation [14,33].
A CWMFC reactor configuration comparable to a built wetland often has its lower anaerobic region filled with a layer of soil, gravel or some other recent substrates, such as activated carbon, zeolite, or alum sludge, as a supporting matrix for the anode electrode. This support matrix also creates the desired environment for the oxidation reaction by microorganisms for contaminant removal and electrons transfer processes [14,16,26,33]. All these components, as mentioned earlier, are essential in the configuration of CW-MFC bioelectrochemical systems. However, to maintain a highly aerobic upper cathodic chamber and increase the removal of pollutants, macrophytes that resource dissolved oxygen to the cathode through plant root respiration are planted in the upper cathode compartment, which contributes significantly to the reduction reaction. Aside from the maintenance of appropriate operating conditions, such as pH, temperature, organic loading rate flow regime, and hydraulic retention rate, the system's physical and biological components' performance is essential for the overall system efficiency [21]. The macrophyte components are recognized to play a pivotal role, as they interact with and influence the performance of all other required components, such as the substrate (filtration media), microorganisms, and electrode materials in CWMFC (see Figure 2). They also influence both the oxidation and reduction reactions. They act as biological filters and accumulators in the treatment process and as oxygenators to the cathode for electricity generation [15]. According to Fang et al. [34], the plants' presence promotes the cathode's oxygen concentration through their photosynthetic activities. In a study by Fang et al. [34], a CWMFC with plants produced an average voltage output of about 15% higher than an unplanted CW-MFC [35][36][37][38].

The Effect of Aquatic Macrophytes on Microbial Organisms
Electroactive bacteria (EAB), or exoelectrogens, are generally recognized as the group of microorganisms that feature an essential role in the performance of CWMFCs. These are bacteria capable of generating electrical energy by organic matter oxidation and moving the electrons produced to an acceptor outside their cells [14,16,38]. In CW-MFC, the presence of macrophytes generally plays a crucial role in enhancing the abundance and diversity of the microbial community in CWMFC and their subsequent effect on contaminant removal and bioelectricity generation [15,37]. Their roots provide a vast surface area for microbial growth and attachment, allowing for the decomposition and uptake of pollutants from wastewater [39]. The efficiency of the system is primarily achieved by sustaining a high redox potential between the upper and lower segments of the CW-MFC. The involvement of aquatic macrophytes is one of the major components known to contribute to root oxygen and exudate secretion. Root oxygen released via plant root respiration, also known as radial oxygen loss (ROL), creates aerobic, anaerobic, and facultative aerobic conditions for various types of microorganisms. These conditions enhance the degradation of organic pollutants by facilitating microbial metabolism [40][41][42][43][44]. In addition, exudates consisting of carbohydrates, amino acids, and water-soluble sugar released by macrophyte roots provide the required carbon source and energy for the microbial metabolism and growth around the rhizosphere of the aquatic macrophyte. This, however, results in a greater relative abundance of EABs, which are responsible for bioelectricity production [37,41,42]. A recent study was undertaken by Wang et al. [45] to assess the influence of aquatic macrophytes on bioelectricity production, contaminant degradation, and micro bacterial community diversity in CWMFCs. The relative abundance of EABs (firmicutes and beta-proteobacteria) in planted CW-MFCs was substantially greater than that of unplanted CW-MFCs, according to the 16S rRNA microbial analysis of their study. These findings indicate that CWMFC with aquatic plants had a significant effect on the exoelectrogens' quantity around the anode material. Subsequently, the higher relative abundance of EABs in the planted CWMFC led to an increase in bioelectricity production of 8.91 mW·m −2 in CWMFC. On the other hand, the highest removal efficiency for COD and nitrate-nitrogen (NO 3 -N) was obtained in photosynthetic CW-MFC with smaller filler sizes of 86.7%, and 87.1%, respectively, which was strongly attributed to the diversity and relative abundance of microorganisms [45]. Similar to this study, Liu et al. [40] performed high-throughput sequencing in their experimentation to assess the effect of different macrophytes and no-macrophyte CWMFC; Canna indica, Acorus calamus, and Ipomoea aquatica on microbial diversity. According to the Shannon diversity indexes (H') of their study, species richness and evenness were relatively higher in planted than unplanted: H' = 4.69. 4.94, 4.99, AND 5.01, respectively, for unplanted, Canna indica, Acorus calamus, and Ipomoea aquatica. More specifically, from the phylogenetic diversity of bacteria, the relative abundances of Geobacter (proteobacteria), the most well-known EAB in the anodes of the unplanted, Canna indica, Acorus calamus, and Ipomoea aquatica systems, were 5.12%, 20.41%, 11.67%, and 20.74%, respectively. This result was noticed to have greatly influenced a higher electrical output and the removal efficiency in CWMFC planted with Canna indica and Ipomoea aquatica than those that were unplanted due to microbial diversity as a result of the plant root exudate and oxygen release. In earlier studies by Shen et al. [44], to investigate the enhanced performance of CWMFC by using wetland plants and enclosed anodes, similar to Liu et al. [40], they used a fluorescence in situ hybridization (FISH) technique to estimate the abundance of Geobacter sulfurreducens and Betaproteobacteria in the anode biofilm; they obtained a positive correlation between the cell voltage and abundance of Geobacter sulfurreducens and Betaproteobacteria, which was 66.22% higher than the unplanted. In 2017, Wang et al. [46] also conducted a similar study to look at the relation between bioenergy generation and rhizodeposition and degradation in CWMFC. Their high-throughput sequencing similarly observed a wide variety of microbes (Clostridium sp., Cytophagales, and Dechloromonas sp., and so forth) in rhizosphere with a higher relative abundance of Desulfobulbus sp. and Geobacter sp. This result was well reflected in the bioenergy production in the planted system with T. orientalis and S. Validus as compared to the unplanted. According to Vymazal [47], the macrophytes' rhizosphere is known to accommodate a wide variety of bacterial forms that make planted CWMFCs outperform those that are unplanted [35,46,48].

The Role of Macrophyte in Bioelectricity Generation
The production of electricity in CWMFC is essentially the result of oxidation-reduction (redox) reactions through electrochemical or biochemical reactions at the anode and cathode chambers, respectively, as shown in Figure 3 [18,21]. The cathode electrode in the cathodic chamber features as an electron donor, whereas the anode fundamentally aids as an electron acceptor [49]. The acceptance of electrons from the cathode electrode for the reduction reaction in the cathode chamber is the responsibility of certain chemical compounds known as terminal electron acceptors (TEA). Transitional metals (e.g., platinum (Pt)) and nitrate (NO 3 ) compounds are materials often used as TEAs [50,51]. Nevertheless, oxygen (O 2 ) is generally recommended due to its sustainable characteristic and relatively high redox potential [51,52], for example, organic matter degradation using glucose as the substrate and oxygen as the electron acceptor.
Therefore, using DO at the system's air-water interface is considered ideal to make oxygen available for this reaction. Given that, some researchers employed air-cathodes to increase the cathodic reduction reaction by engaging the cathode material at the air-water interface. However, this arrangement was affected by huge ohmic losses because such an arrangement widens the distance between electrodes (the anode and the cathode), which increases the internal resistance of the cell [22,23]. In addition to the air-cathode that suffered setbacks of increased internal resistance, some other researchers also employed supplemen-tary artificial aeration. According to Guo et al. [53], the supplemental artificial aeration was recognized as a form of energy wastage and inhibited the denitrification process. The use of macrophytes to meet this major requirement became a huge breakthrough for the optimal functioning of the system. The prerequisite for oxygen without any setbacks in the upper cathodic region for the mentioned reduction reaction makes the macrophyte component a significant part of the system to guarantee optimum performance and efficiency [14]. The macrophyte plants use sunlight as an energy source during photosynthesis to turn water and carbon dioxide into chemical energy (glucose), and the by-product is gaseous O 2 in their rhizosphere, which is then made available as TEAs to complete the energy production cycle in the system [54] as shown in Figure 4. Oxygen is made available for the reduction reaction by utilizing the oxygen excreted by the macrophyte roots within the cathode zone. Research has shown that with such incorporation, the wetland plant improves the cathode potential of the CW-MFC for optimum energy production. In an experiment conducted by Liu et al. [48], they compared CW-MFC planted with Ipomoea aquatica and unplanted CWMFC systems. It was evident from their study that the plants' presence can boost the system's electricity production by 142% [48]. They attained a maximum power density (P D ) of 12.42 mW·m −2 generated from the system incorporated with I. aquatica, which was 142 percent greater than the 5.13 mW·m −2 generated from the system without aquatic macrophytes [48]. It was also observed by Saba et al. [55] that Ipomoea aquatica in CWMFC improved power generation from 0.191 to 0.302 W·m −3 by improving the cathode potential from 0.522 V to 0.603 V as a result of the oxygen availability by the plant root [35,54]. This result agrees with earlier experimentation from Shen et al. [44], where, to ascertain the contribution of Hydrilla verticillata in a CWMFC with regards to DO fixation, they observed that the DO of the planted system was 9 times higher than the unplanted. Further studies by Liu et al. [40] also assessed the DO concentration of four CWMFCs: unplanted CWMFC, and three CWMFCs planted with Canna indica, Acorus calamus, and Ipomoea aquatica. The DO concentration of the unplanted was remarkably lower (1.95 mg/L) than that of the planted. However, among the planted, the effluent of CWMFC planted with Canna indica demonstrated the highest DO concentration (3.31 mg/L), which indirectly led to higher bioelectricity production than the unplanted. Similarly, Di et al. [42] also assessed the influence of plant radial oxygen loss (ROL) in CWMFC in the removal of nitrobenzene from an aqueous solution. They compared three macrophyte species: Scirpus validus, Typha orientalis, and Iris pseudacorus, with unplanted CWMFC. Generally, their results demonstrated that the planted CWMFC were more efficient in power production and nitrobenzene removal than unplanted. However, relatively, Scirpus validus attained the highest power density 19.5 mW/m 2 due to its high DO production of 2.57 ± 0.17 mg/L and root biomass of 16.42 ± 0.18 g·m −2 . In CWMFC, because of root oxygen exudation, aquatic plants play a crucial role in achieving a high oxidation-reduction potential between the two regions. In comparing the contribution of macrophytes in the creation of redox conditions necessary for power production, Doherty et al. [33] in their experiment, also reported that the presence of macrophytes had a more significant impact on redox conditions by creating a redox gradient that was 20 percent greater than that of the unplanted. Likewise, in a study conducted by Corbella et al. [56], the macrophytes' overall influence on redox conditions within the wetlands was analyzed by contrasting the planted and unplanted wetlands wrought under a continuous flow regime. Their results proved that plants increased the redox gradient with a subsequent energy production of 16 mW/m 2 higher than the unplanted. Similarly, Oon et al. [54], in their study to assess the role of macrophytes in an up-flow CWMFC by comparing planted with unplanted systems, established that DO at the cathodic region for planted systems was higher with a high reduction potential, compared to unplanted, making the planted system perform better than the control (unplanted) in their electrical output. The main transport mechanisms of oxygen in macrophyte CWMFC is by the air pressure gradient from the atmosphere and mainly via diffusion from the aerenchyma cells of roots during plant photosynthesis [39,40,57]. The oxygen secreted from the root aerenchyma cells creates a micro-oxidizing environment in the rhizosphere; hence, the redox potential of the system is greatly influenced [58,59]  However, aside from plant root oxygenation, the secretion of exudates also plays a crucial role in bioelectricity production. Root exudates secreted from wetland plants are organic matter, and electroactive bacteria that form naturally within the anode region oxidize the exudates, producing electrons for the production of bioelectricity. Hence, macrophyte species with such propensity will have a great influence on the system's electrical output [59]. Guadarrama-Perez et al. [60] investigated the effect of four different native species-Aglaonema commutatum, Epipremnum aureum, Dranacaena braunni, and Philodendron cordatum-as the only endogenous substrate from their root exudate and the effect on bioelectricity production. Philodendron cordatum attained the highest power density of 12.5 mW·m −2 with a 20.6 mg/L maximum production of root exudates. Therefore, at an optimal condition for root exudate release, the CWMFC performance in terms of bioelectricity was observed to be higher. Different species based on their substrate type, transport, and exudate release will have a different effect as demonstrated by [60]. Zhou et al. [61] also investigated the effect of root exudates and oxygen release by four wetland plants, Canna indica, Cyperus alterniferlius, Acorus calamus, and A. donax, with unplanted CWMFC on power production. The A donax macrophyte species attained the highest maximum power density of 12.82 mW·m −2 compared to the unplanted and the remaining species. From their results, they concluded that the relatively high rate of root exudation and oxygen release by aerenchyma was generally responsible for the high electrical output in A. donax. It is evident that wetland plants in CWMFC play a crucial role in power production. It is, therefore, highly imperative that the incorporation of macrophytes in CWMFC should be considered to ensure optimal power production. However, in the selection of such species, ROL and exudate production based on the photosynthetic process of the plant should be well considered. Further details on different types of macrophytes with their contribution to electricity generation are itemized in Table 1 [54].

Role of Macrophyte in CW-MFC Contaminant Removal
In many wastewater effluents, high COD levels, nutrients such as ammonium-nitrogen, nitrate-nitrogen, and phosphorus, and heavy metals are common pollutants. CWMFC as an eco-friendly technology can remove, transform, and immobilize these and many other wide ranges of contaminants [62]. The efficacy of CWMFC in wastewater treatment, however, depends on multifaced interacting processes that can be narrowly divided into three major categories-physical, chemical, and biological-as shown in Figure 5 below [21,35,54,62]. The macrophyte component plays a versatile role in all of these processes to ensure the effective removal of wastewater contaminants. For example, in the bioaccumulation and phytoremediation process, aquatic plants have an important role in the absorption, assimilation, and storage of nutrients in their biomass, either directly or indirectly. They sequester soluble inorganic nutrients as a means of building their biomass [21,24,62,63]. Since these nutrients are necessary for their growth, the amount of nutrients from wastewater effluent can be maximized by choosing wetland plants with a high capacity for inorganic absorption and their subsequent conversion to organic plant biomass [57]. In addition, besides the release of oxygen (oxygenation) from plant roots to maintain a redox condition necessary to facilitate nitrification by aerobic microbes, the root system of submerged and emergent aquatic macrophytes releases exudates through a process called "rhizodeposition" [18,25,33,64]. These rhizodeposits act as an energy source for denitrifiers for the denitrification processes for the effective removal of nitrate in wastewater [18,64,65]. Organic rhizodeposits range from low molecular weight exudates to full roots used as energy sources for microorganisms in the CWMFC [17,62]. In a study conducted by Wen et al. [64] to assess the influence of macrophytes in ammonium nitrogen (NH 4 + -N), nitrate, and total nitrogen removal, they used Canna indica, a typical wetland plant, and their results showed that the planted CW-MFC was 38% higher in removal efficiency than unplanted CW-MFC. This was mainly because the wetland plant enhanced the nitrification process. In a similar study, comparing planted CWMFC with unplanted CWMFC to investigate the role of plants (Canna indica) in the treatment of antibiotics and NH 4 + -N, the presence of plants was observed to have accelerated the accumulation of sulfamethoxazole, tetracycline, and NH 4 + -N [59]. In addition to their inimitable role as bio accumulators, wetland plants also play an essential filtering and adsorption role by reducing wastewater's velocity and allowing finer particles to adhere to plants' biofilm surfaces. It is undoubtedly proven that the wetland plant species significantly affect pollutant removal in CWMFC [17,[62][63][64]. In a study conducted by Liu et al. [48] to enhance the efficiency of CWMFC using plant photosynthate, the root exudates of Ipomoea aquatica were utilized as a part of the fuel in photosynthetic MFC to enhance denitrification [48]. Phragmites australis had better removal of NH 4 -N than Iris pseudacorus and was able to enhance the rhizosphere's nitrification process due to the more robust radial oxygen loss (ROL). A recent study by Oon et al. [54] using Elodea nutallii CWMFC operated under artificial aeration attained 98% COD removal efficiency. In another recently conducted experiment in South Africa by Oodally et al. [66], to examine the contribution of macrophytes used in CWMFC, they compared three indigenous South African species: Phragmites australis, Cyperus prolifer, and Wachendorfia thyrsiflora with an unplanted system as control. They obtained a COD removal efficiency of 97 ± 1% for Cyperus prolifer, which was higher than P. australis, Wachendorfia hyrsiflora, and the unplanted with 94 ± 1%, 94 ± 1%, and 90 ± 2% removal efficiencies, respectively. The Cyperus prolifer species attained a higher orthophosphate removal efficiency (98 ± 0%) than the control experiment (72 ± 7%), Wachendorfia thyrsiflora (58 ± 6%), and Phragmites australis (81 ± 4%). Comparatively, Cyperus prolifer was noticed to be the most suitable indigenous wetland plant for electricity production and COD, ammonia, and phosphate removal among the three species [66]. These results also justify Greenway's [62] assertion that different macrophyte species differ in their removal efficiency, hence the need for a proper investigation in selecting macrophyte species to be incorporated into the CWMFC system. Furthermore, studies on plant effects of nitrogen, phosphorus, and heavy metal removal are scanty, compared to the COD removal efficiency. Although generally proven under constructed wetlands (CW), extensive studies are required regarding CWMFC [63]. This study may help optimize the integration of the plant component in CWMFCs and boost the development of CWMFCs for practical use (see Table 1).

Future Perspective
In a CWMFC configuration, the appropriate selection of macrophytes is crucial for the system's success. The macrophyte component is one of the most conspicuous and versatile parts of the CWMFC bio-electrochemical system. The types of wetland plants installed in CWMFCs have some unique properties that make them play such an essential role in the contaminant removal processes and bioelectricity generation [44][45][46][47]66]. Therefore, a thorough selection of the type of macrophyte to be used is hugely imperative to the system's success. This decision can either enhance or retard CWMFC's efficiency significantly. Hence, an appropriate selection of wetland plants must be based on some unique characteristics, such as those outlined in Table 2. Table 2. Characteristics of macrophytes and their relevance in CWMFC.

Macrophyte Properties
Relevance in CWMFC rapid growth and high biomass production For winter insulation in cold and temperate regions, and particularly for the removal of nutrients by harvesting, as nutrients are absorbed by macrophytes to build their biomass [47]. In addition, according to Yang et al. [76], species with high biomass production in CWMFC enhance the cell voltage and reduce the internal resistance of the system, which often results in higher bioenergy production.
good natural adaptation to the local climate Native species should be best preferred. According to Sierra et al. [25], CWMFC plants are selected based on the region's most common aquatic plants. Oodally et al. [66], also concluded that native species are best preferred due to their local climate adaptability. In their experimentation, the most common aquatic plants in the region showed improved performance in CWMFC than exotic species.

good root development
To provide a substrate for attached bacteria and oxygenation [46,47]. Additionally, the root development or maturity of the wetland plant affects oxygen release. In a sediment microbial fuel cell (SMFC) with wetland plant experiments conducted by Chen et al. [58], their investigation has shown that young roots can excrete more oxygen than mature or aging species. Similarly, Colares et al. [41] also observed that plant species with good root development produced better oxygen, which presented the highest voltage value, compared to plants with smaller poor root systems. In addition, Moqsud et al. [77] operated a series of 6-CWMFC using Oriza sativa species. In their experimentation, they observed a reduction in power production as plants attained maturation. This was mainly because the maturation of the plant affected both oxygen release and exudate production. This signifies that the maturity of the root and its development is an essential factor in wetland plant selection [58].

High oxygen transfer capacity
Oxygen transfer capacity from the roots creates an aerobic environment. Due to the great diversity of flora, different species have different radial oxygen loss (ROL) [25].

nutrient absorption capacity
High nutrient absorption capacity helps in the effective removal of contaminants from the system. Species with high NAC use absorbed nutrients as a resource for their metabolism and growth [48,62].
adaptation and ease of propagation The ease in obtaining seedlings, seeds, or vegetative propagules must be well considered to ensure system sustainability. Good Rhizodeposition; release of carbon sources as rhizodeposits from plant roots.
Rhizodeposition supports the growth and activities of microorganisms associated with bioelectricity production [78].

C4 Plants
The photosynthetic activity of plants is categorized into three phases: C3, C4, and CAM. In terms of oxygen production and CO 2 fixation, plants in each category have different photosynthetic pathways. Plants in the group of C4 are those with more advanced photosynthetic activity than plants in the C3 and CAM groups. Consequently, because they have a higher conversion rate of solar energy into bioelectricity, it is suggested to integrate C4 plants [18].
These factors should be primarily considered in the appropriate selection of macrophytes for CWMFC. Nevertheless, owing to the wide variety of aquatic flora, further investigation is needed to evaluate and select plant species with potential for CWMFC for simultaneous wastewater regeneration and bioelectricity production [79].
Macrophytes, particularly emergent plants, can cause substantial water loss in CWMFC through evapotranspiration. As the volume of wastewater flowing through the system decreases due to water loss, the treatment efficiency in CWMFCs could be affected significantly when the evapotranspiration rate exceeds 2.5 mm/d [54,80]. In addition, in the absence of light, plant cells and microorganism respiration consume O 2 . Hence, the DO level in the reactor will be reduced as the DO consumption rate exceeds production. The plant's photosynthesis and respiration alters the reactor's oxygen dynamics, ultimately leading to voltage fluctuations [22]. Therefore, macrophyte species that can help overcome these major setbacks are highly recommended.

Conclusions
From all previous and recent studies on the plant component of the CWMFC, there is no doubt that macrophytes have a significant effect on the performance of CWMFC for wastewater treatment and electricity generation. However, most of the studies conducted to assess the macrophytes' impact on treatment efficiency focused on COD removal, with less focus on nitrate, phosphorus, and heavy metal removal through the selection of appropriate macrophytes. Previous studies also focused on general macrophyte performance on treatment efficiency and power generation performance without comparing different species performance and plant hybrid integration of CW-MFC performance. This comparison and analysis will help in recommending the right wetland plant for the technology based on plant performance to filter contaminants, absorb heavy metals, excrete more dissolved oxygen needed to increase the system's redox potential, and aid in the removal of other pollutants. More macrophyte species must be explored to compare different species' performance on the system. Studies must also be conducted on the pollutant effect on the plant component of CWMFC, i.e., ascertaining the phytotoxicity caused by contaminants.