The Influence of Different Pre-Treatments on the Quality and Nutritional Characteristics in Dried Undersized Yellow Kiwifruit

Undersized kiwifruits are considered waste; therefore, there is a need to develop sustainable processing technologies for their valorization, in order to have a positive economic impact on their entire production chain. Therefore, the aim of this study was to apply pulsed electric fields (PEF) combined with osmotic dehydration (OD) and air drying (AD) to develop a novel yellow kiwifruit dried snack with high nutritional functionality. In particular, the effect of the different pre-treatments, PEF (200 V/cm) and/or OD (trehalose at 40%), on the color, bioactive compounds content and antioxidant activity (AA) was evaluated on kiwifruit chips air-dried at three different temperatures (50, 60, 70 ◦C). The results showed that better color maintenance was observed at the drying temperature of 60 ◦C. Concerning the bioactive compounds, a good retention in total polyphenols (TP), vitamin C content and AA with the DPPH method was obtained on OD and PEF kiwifruit dried snacks, similar to that of control samples. Instead, the ABTS method showed the best AA in PEF and OD/PEF samples. In general, a shorter drying time in the ODand PEF-treated samples could contribute to the higher sustainability of the process.


Introduction
Nowadays, owing to the growing consciousness of environmental pollution and the high amount of produced waste during conventional food processing, consumers appreciate sustainable food productions [1]. The improvement and/or removal of one of the conventional technological steps applied in food processing can be a promising strategy in order to increase the environmental benefits and the raw material processing efficiency [2]. Moreover, consumers are increasingly interested in food products with improved safety, as well as healthy and nutritional aspects [3].
The changes in consumer perception and the need to produce safe and high-quality foods are responsible for the evolution of the established food processes or the development of new ones. Wastage arises in particular from fruit and vegetable processing or from fruits with unfitting characteristics, in terms of size and shape [4]. Since most of these products are not used for fresh consumption, they may be inappropriately disposed of, causing environmental issues. Consequently, the opportunity to reuse industrial loss and/or waste by improving process profits and sustainability is of great relevance.
According to the European Commission [5], the weight of kiwifruit must be above 65 g, otherwise they are considered not adequate for the commercial sale and the distribution channel, thus fruitless. However, those fruits could be valorized as raw material for the production of snacks by reducing food waste and increasing their added value. Kiwifruit is a good source of antioxidant compounds such as vitamin C and polyphenols, which contribute to its high antioxidant potential [6].
Commonly, freeze-drying or conventional drying have been used to obtain stable fruit products; however, these techniques present limitations such as high energy consumption and long drying times [7][8][9]. Moreover, drying at elevated temperatures causes irreversible damage owing firstly to the changes in cell wall and cell membrane structures that are followed by changes in the functionality of nutritious components (vitamin C, antioxidants), mainly due to oxidation [9].
In general, drying processes consume an appreciable part of the total energy used in the food industry, so it is very important to develop new hybrid drying technologies both for energy saving and preservation of the final food quality [10]. Pulsed electric field (PEF) and osmotic dehydration (OD) treatments can be used in combination owing to their modulation of specific structural features of plant tissue, enhancing the nutritional functionality of the final product [11,12]. The PEF treatment at high and moderate fields has been suggested to enhance the maintenance of nutritional components during mass transfer processes in different types of fruit [13][14][15][16], while OD treatments have been used for the retention of flavor, color, texture and inhibition of enzymatic browning [17].
In this direction, this research work was aimed at developing a novel fruit snack for the valorization of undersized kiwifruit, assessing different sustainable processing solutions. Two non-thermal technologies, such as OD and PEF as a pre-processing step to drying, were applied alone or in combination, resulting in a novel dried kiwifruit product with a short processing time and good quality, in terms of color and nutritional characteristics (antioxidant activity, total polyphenol and vitamin C content) retention.

Sample Preparation
Undersized kiwifruits Actinidia chinensis cv "Jintao" (average weight of 60.7 ± 1.6 g, 13 ± 1 • Brix) were obtained from the Jingold Consortium (Cesena, Italy). Fruits were stored at 4 ± 1 • C until they were used, for a period not longer than one week. Fruits were washed, peeled and sliced in order to obtain discs of 3 mm thick.
Kiwifruit discs were subjected to the OD process and/or PEF treatment with different combinations, selected on the basis of preliminary experiments, as reported in Table 1. Different sequences of the combined treatment were applied such as OD followed by PEF, PEF followed by OD or PEF followed by OD and again PEF treatment, in order to clearly understand the differences in terms of treatment effect through the different work sequences applied on the food product. OD was caried out in 40% (w/w) trehalose solution at 35 • C for 2.5 h, showing a good efficiency of the process, reflected in a Appl. Sci. 2020, 10, 8432 3 of 13 high water loss/solid gain ratio in the range of 3.5-3.6.Trehalose was chosen because of its potential positive effect in the preservation of biomolecules and low cariogenic potential when compared to sucrose [18,19]. The treatment was performed in continuous stirring using a fruit/OD solution ratio of 1:4, in order to avoid concentration changes of the solution during the treatment [11].
PEF treatment was applied by using a pulse generator, S-P7500 60A 8 kV (Alintel srl., Bologna, Italy). The following process parameters were used: electric field strength of 200 V/cm, and 1000 nearrectangular-shaped pulses, with a fixed pulse width of 10 µs and a frequency of 100 Hz. The total treatment time was set to 10 s and total energy input was of 1.92 kJ/kg. Table 1 summarizes the pre-treatment applied and the samples obtained at each drying temperature. Table 1. Overview of the combination of treatments applied for kiwifruit slices at each drying temperature (50, 60 and 70 • C) and relative sample codes.

Treatment
Sample Code The differently pre-treated samples were subjected to air drying at 50, 60 and 70 • C, until a target water activity of 0.2 was reached. The drying was performed in a tray drier, CLW 750 TOP+ (Pol-Eko-Aparatura SP.J., WodzisławŚląski, Poland), with transverse air flow, air velocity of 2 m/s and an air renewal fee of 50%.
The total time of the drying processes varied as a function of the applied pre-treatment and temperature, as reported in Table 2. Water activity (a w ) was measured with a water activity meter, AquaLab Series 3TE (Decagon Devices, Inc., Pullman, WA, USA), at room temperature. The measurements were conducted in triplicate.
The analyses were carried out at least in ten repetitions from each sample at each drying temperature.

Functional Components Content and Antioxidant Activity
The extraction was performed by mixing 0.5 g of dried kiwifruit sample with 10 mL of methanol 60% (w/w) in a centrifuge tube. The mixture was vortexed for 2 min, agitated for 10 min and centrifuged for 10 min at 18,600 rpm in a centrifuge (Beckman, Brea, CA, USA) set at 4 • C [20]. The supernatants were collected and used to evaluate the total polyphenol, vitamin C and antiradical activity by DPPH and ABTS assays.

Total Polyphenols (TP)
The total polyphenol content (TPC) of kiwifruit samples was determined according to the Folin-Ciocalteu method, as described by Tylewicz et al. [20]. The TP content was expressed as mg of gallic acid equivalents (GAE) per 100 g of dried sample based on the standard curve obtained with gallic acid (R 2 = 0.993). The values provided are the average of three replicates from each sample at each drying temperature.

Vitamin C Content
The determination of vitamin C concentration was carried out by a redox titration using iodine solution (0.005 mol/L). As the iodine was added during the titration, the ascorbic acid was oxidized to dehydroascorbic acid, while the iodine was reduced to iodine ions. Due to that, when all the ascorbic acid was oxidized, the excess iodine was free to react with the starch indicator by forming the blue starch-iodine complex that represent the endpoint of the titration [21]. Vitamin C content of the air-dried kiwifruit slices was expressed as mg of ascorbic acid per 100 g of dry matter of kiwifruit. The values provided are the average of three replicates from each sample at each drying temperature.

Antioxidant Activity (DPPH and ABTS Assay)
The DPPH scavenging activity was based on the method proposed by Amarowicz et al. [22]. Briefly, 0.1 mL of kiwifruit extract was added to 2 mL of methanol and 0.25 mL of DPPH (Sigma-Aldrich, St. Louis, MO, USA), shaken with a vortex for 1 min and kept in the dark for 30 min. The absorbance was measured with a spectrophotometer (Beckman Coulter DU 730 Life Science model) at 517 nm. Antioxidant activity was quantified by Trolox (Sigma-Aldrich, Milan, Italy) as an external standard. The results were expressed as µmol Trolox/100 g of dry matter of fruit.
The ABTS+ scavenging activity was carried out following the method proposed by Re et al. [23]. Here, 30 µL of extract was added to 3 mL of diluted ABTS+ solution (Sigma-Aldrich, St. Louis, MO, USA) after mixing and the absorbance was measured with a spectrophotometer (Beckman Coulter DU 730 Life Science model) at 734 nm every 30 s for a total time of 6 min; the results were expressed as µmol Trolox/100 g of dry matter of fruit. Antioxidant activity was quantified based on the standard curve obtained with Trolox (Sigma-Aldrich, Milan, Italy). The values provided are the average of three replicates from each sample at each drying temperature.

Statistical Analysis
All obtained data were evaluated and discerned by using an analysis of variance (ANOVA) followed by Tukey's HSD post hoc test to compare the means at the level of confidence of 95% (p < 0.05). The analysis was performed using the software STATISTICA 6.0 (Statsoft Inc., Tulsa, UK).

Color
Fresh kiwifruit samples were characterized by L*, h • and C* color parameter values equal to 43.8 ± 3.1, 88.4 ± 1.2 and 23.9 ± 1.9, respectively, while the color parameters of dried kiwifruit snacks are reported in Table 3. As it can be seen from Table 3, the different pre-treatments had an influence on the final color of the kiwifruit snacks. The pre-treatment with OD and PEF alone did not promote any changes in color parameters in samples dried at three different temperature, while a decrease in the luminosity of the samples pre-treated with the combination of OD/PEF and PEF/OD was observed. Concerning the hue (h • ) angle color parameter, no significant differences were found between the different samples, while the chroma parameter (C*) was found to decrease in the OD/PEF and PEF/OD samples dried at the lowest and highest temperatures. In general, the drying temperature of 60 • C seems to be the most suitable for the maintenance of the kiwifruit fresh-like color. Table 3. Color parameters (L*-luminosity, h • -hue angle, C*-chroma) of untreated and differently pre-treated kiwifruit snacks dried at the temperatures of 50, 60 and 70 • C.

Total Polyphenols
Total polyphenols contents in kiwifruit dried snacks are shown in Figure 1. The highest content of TP (2058 mg GAE/100 g dry matter (dm)) was observed in the C kiwifruit snack dried at 60 • C, followed by the samples treated with OD and dried at the same temperature of 60 • C (1311 mg GAE/100 g dm). PEF pre-treatment of kiwifruit slices alone or in combination with OD promoted a significant decrease in TP values, showing the lowest values in samples dried at 60 • C which is an opposite trend to the one observed in C and OD samples. Finally, the snack pre-treated with PEF, OD and PEF again (PEF/OD/PEF) seems to not undergo variations in polyphenols among the different used drying temperatures, showing, however, the lowest TP values in comparison to the other treated samples. Regarding the temperature applied during the drying process, the highest retention of TP was observed for C samples, respectively, at 50 and 60 • C and for the OD one dried at 70 • C, although long drying times were recorded at the lowest temperatures (respectively 960, 480 and 240 min). Appl. Sci. 2020, 10, x FOR PEER REVIEW 6 of 13

Vitamin C Content
The vitamin C contents of differently pre-treated kiwifruit chips dried at different temperatures are shown in Figure 2. The highest vitamin C content was observed in C kiwifruit slices dried at all the used temperatures and on OD and PEF pre-tretaed snacks dried at 70 and 50 °C, respectively. The vitamin C content was strongly influenced by the pre-treatment applied on the kiwifruit chips. In fact, it decreased as the number of processing steps increased (from 46.48 to 21.82 mg ascorbic acid/100 g dm for OD and PEF/OD/PEF treated samples, respecively, dried at 50 °C). Except for sample PEF, in general, a better retention of vitamin C was observed with an increase in the temperature (e.g., from 21.63 to 29.27 mg ascorbic acid/100 g dm for PEF/OD chips dried at 50 and 70 °C, respectively).

Vitamin C Content
The vitamin C contents of differently pre-treated kiwifruit chips dried at different temperatures are shown in Figure 2. The highest vitamin C content was observed in C kiwifruit slices dried at all the used temperatures and on OD and PEF pre-tretaed snacks dried at 70 and 50 • C, respectively. The vitamin C content was strongly influenced by the pre-treatment applied on the kiwifruit chips. In fact, it decreased as the number of processing steps increased (from 46.48 to 21.82 mg ascorbic acid/100 g dm for OD and PEF/OD/PEF treated samples, respecively, dried at 50 • C). Except for sample PEF, in general, a better retention of vitamin C was observed with an increase in the temperature (e.g., from 21.63 to 29.27 mg ascorbic acid/100 g dm for PEF/OD chips dried at 50 and 70 • C, respectively).

Vitamin C Content
The vitamin C contents of differently pre-treated kiwifruit chips dried at different temperatures are shown in Figure 2. The highest vitamin C content was observed in C kiwifruit slices dried at all the used temperatures and on OD and PEF pre-tretaed snacks dried at 70 and 50 °C, respectively. The vitamin C content was strongly influenced by the pre-treatment applied on the kiwifruit chips. In fact, it decreased as the number of processing steps increased (from 46.48 to 21.82 mg ascorbic acid/100 g dm for OD and PEF/OD/PEF treated samples, respecively, dried at 50 °C). Except for sample PEF, in general, a better retention of vitamin C was observed with an increase in the temperature (e.g., from 21.63 to 29.27 mg ascorbic acid/100 g dm for PEF/OD chips dried at 50 and 70 °C, respectively).

Antioxidant Activity (DPPH and ABTS Assay)
The antioxidant activity (AA) values measured by both DPPH and ABTS methods on differently obtained kiwifruit chips samples are shown in Figures 3 and 4, respectively. The behavior of the antiradical activity in all obtained samples is strongly dependent on both the explored pre-drying treatments and their combinations and on the applied drying temperature. The highest retention of bioactive compounds with the DPPH method ( Figure 3) was observed in the C sample dried at 60 • C, followed by the OD sample treated at the same temperature. PEF treatment alone and OD/PEF also showed a moderate antiradical DPPH activity, especially when the pre-treated kiwifruit chips were subsequently treated at the highest temperature of 70 • C. However, chips treated with PEF/OD and PEF/OD/PEF, independently of the drying temperature used, had a significantly lower AA in comparison with other kiwifruit samples. In particular, the lowest scavenging activity was observed in PEF/OD and PEF/OD/PEF respectively dried at 70 and 60 • C (589 and 538 µmolTrolox/100 g dm).
Appl. Sci. 2020, 10, x FOR PEER REVIEW 7 of 13 between all considered samples at each drying temperature, while capital letters indicate significant differences (p < 0.05) between each sample at the three drying temperatures.

Antioxidant Activity (DPPH and ABTS Assay)
The antioxidant activity (AA) values measured by both DPPH and ABTS methods on differently obtained kiwifruit chips samples are shown in Figures 3 and 4, respectively. The behavior of the antiradical activity in all obtained samples is strongly dependent on both the explored pre-drying treatments and their combinations and on the applied drying temperature. The highest retention of bioactive compounds with the DPPH method ( Figure 3) was observed in the C sample dried at 60 °C, followed by the OD sample treated at the same temperature. PEF treatment alone and OD/PEF also showed a moderate antiradical DPPH activity, especially when the pre-treated kiwifruit chips were subsequently treated at the highest temperature of 70 °C. However, chips treated with PEF/OD and PEF/OD/PEF, independently of the drying temperature used, had a significantly lower AA in comparison with other kiwifruit samples. In particular, the lowest scavenging activity was observed in PEF/OD and PEF/OD/PEF respectively dried at 70 and 60 °C (589 and 538 μmolTrolox/100 g dm).

Discussion
It has been reported in the literature that different pre-treatments could have an influence on the color parameters of the treated products. Tylewicz et al. [24] showed an increase in the luminosity (L*) of kiwifruit samples treated with OD in trehalose solution, while no differences were observed However, AA measured with the ABTS method ( Figure 4) showed the highest values, from 3261 to 4727 µmolTrolox/100 g dm, in PEF and OD/PEF kiwifruit samples dried at all three drying temperatures. Conversely, the lowest values were observed for OD chips dried at 50 and 60 • C and for C, OD, PEF/OD and PEF/OD/PEF kiwifruit samples dried at 70 • C.

Discussion
It has been reported in the literature that different pre-treatments could have an influence on the color parameters of the treated products. Tylewicz et al. [24] showed an increase in the luminosity (L*) of kiwifruit samples treated with OD in trehalose solution, while no differences were observed when sucrose was used in the osmotic solution. Similarly, the PEF pre-treatment alone resulted in significant changes in the color parameters of kiwifruit samples [25]. However, in the present work, the effect of the pre-treatment was evaluated only in the combination with the drying process and did not show any statistical differences. The decrease in luminosity observed in the kiwifruit snack samples pre-treated with the combination of OD/PEF and PEF/OD and dried at 50 and 70 • C could be due to the increased release of enzymes, such as peroxidase (POD) and polyphenoloxidase (PPO), and their substrates induced by the electroporation of the cell membrane [26] and by the leaching of pigments during osmotic dehydration [26]. Moreover, those samples also presented lower chroma parameter (C*) values which indicates a loss of color intensity. The drying temperature of 60 • C seems to be the most suitable for the maintenance of the kiwifruit fresh-like color. However, the color differences between the various samples, although significant in certain cases, do not indicate a negative effect of any of the applied treatments.
Fruits and vegetables are good sources of natural antioxidants, containing carotenoids, vitamins, phenolic compounds, folate and flavonoids. One of the reasons why kiwifruit is widely appreciated by consumers is related to its high nutritional quality.
Polyphenols are compounds which can influence the characteristics of plant materials, including color, bitterness and antioxidant activity [27].
Raw kiwifruit from the Jintao cultivar is particularly rich in polyphenols and, according to the literature, presents a value of TPC ranging from 139.5 to 143.8 mg GAE/100 g [28].
As previously summarized, both pre-treatments and temperatures applied in this work had a negative effect on TPC except for the kiwifruit chips exposed to the OD treatment and dried at 60 • C. In particular, it appears that PEF alone and in combination with OD as well as thermal treatment could influence the PPO activity by enhancing its reaction with polyphenols and oxygen, which involves the oxidative degradation of phenolic compounds as explained by Yu et al. [29]. Conversely, higher TPC was observed for dried goji berries pre-treated with the combination of PEF (at different field strengths of 0.9, 1.8 and 2.8 kV/cm) and OD (60 min at 55 • C in a hypertonic multi-component solution) processes when decreasing the drying time at 60 • C [15].
Yu et al. [29] reported a loss in TP of around 48.8% for blueberries pre-treated with PEF at 3 kV/cm, with 200 pulses per second, a pulse width of 1 µs and total treatment time of 5 min subsequently subjected to the OD process (fruit/syrup ratio of 1:2 at 40 • C), without further drying.
Kiwifruit is rich in vitamin C, which represents the prevalent nutritional compound in this type of fruit; indeed, it accounts for about 130 to 170 mg/100 g and 68 to 104 mg/100 g, respectively, in Jintao and Hayward cultivars at the harvest time [30]. Moreover, vitamin C is a compound sensitive to oxidative reactions, which may be triggered by the high temperature and the presence of oxygen, so for this reason, it can be considered as an indicator of the overall quality of this kind of fruit during processing.
It is stated from the literature that the increasing drying temperature and time provoke a decrease in vitamin C content in different types of fruit [31][32][33]. On the contrary, in the present study, in most of the samples with the increasing drying temperature, a higher vitamin C content was observed; this result could be due to the reduced treatment time (necessary to reach a w = 0.2) and thus to a shorter exposure to the highest temperature. Moreover, the lower number of processing steps such as in the case of the control sample (C) or of those subjected to a single pre-treatment (OD and PEF samples) allowed a better retention of vitamin C and probably even other valuable compounds. In fact, OD and PEF kiwifruit chips dried, respectively, at 70 and 50 • C showed ascorbic acid values significantly similar to those of C samples.
These results clearly showed that the number of processing steps together with an oxygen-rich environment could significantly reduce the ascorbic acid content.
The AA, assessed with both DPPH and ABTS methods, revealed different results owing to the different capabilities of the two assays to detect certain valuable components. In fact, DPPH is more prone to identify flavonoids compounds, while the ABTS method seems to be more suitable for vitamin C [34].
Indeed, a higher retention of bioactive compounds by the DPPH assay was observed for C and OD chips dried at 60 • C, which is in line with the TPC results observed in the same dried samples (R 2 of 0.77 and 0.70, respectively). The AA results obtained with the ABTS method were higher in the kiwifruit chips pre-treated only with PEF and dried at all three temperatures and with the combination of PEF/OD after drying at 70 • C. These findings were not affected by the observed values of vitamin C content, expect for the PEF-treated chips dried at 50 • C (R 2 of 0.70).
Moreover, the changes in the cellular membranes may have induced a greater release of bounded antioxidant compounds, making them more accessible during the extraction step before the assays of both methods. Similar results were reported by [16], who investigated the application of PEF (2.8 kV/cm, 750 pulses) and OD (55 • C, 60 min) prior to the drying process at 60 • C in a goji berry product.
Huang et al. [35] reported a high retention of bioactive compounds assessed by the DPPH method in apricot pre-treated with PEF (at 1.25 kV/cm, 30 s, 100 Hz and pulse width of 20 µs) and subsequently subjected to soaking in a 0.2% sodium sulfite solution (fruit/solution ratio of 2:5) for 1 h and finally hot air-dried at 45 • C.
As for the intensity of the PEF treatment, Tylewicz et al. [24] observed a significant increase, around 15.7%, in the antioxidant activity assessed by DPPH in strawberry tissues treated at 200 V/cm and 10 µs (total treatment time of 10 s and specific energy of 1.92 kJ/kg), while for kiwifruits, lower intensities of PEF (100 V/cm, 0.96 kJ/kg) were found more beneficial on the retention of antioxidant compounds (7%). However, the combination of PEF at 100 and 200 V/cm and OD with trehalose solution (40%) caused lower antioxidant activity in both strawberry and kiwifruit samples compared to related untreated tissues.
The application of different pre-treatments caused a reduction in the drying time. This reduction was higher when the lowest temperature of 50 • C was used (62-68%), while for the samples dried at 60 and 70 • C, the reduction in drying time was of 25-50% and 28-57%, respectively. A reduction in drying times of 30-60% was also observed by Osae et al. [36] and Bialik et al. [37], respectively, for ginger slices and kiwiberry. Moreover, OD treatment can reduce the total energy consumption (two-three times) if compared to convective drying without any pre-treatment [38]. Similarly, PEF pre-treatment, due to the electroporation that takes place in the whole treated product, can also cause the enhancement of mass transfer and consequently a reduction in the drying time [15,16]. Thus, the reduction in the process time, especially for the lower drying temperature, for both the OD and PEF pre-treatments would bring a significant reduction in the total request of energy even considering the energy input for the PEF treatment (1.92 kJ/kg in our experiments), meaning that it could be an important contribution to improve the process sustainability as a "greener" drying process. Regarding the water consumption involved in the OD pre-treatment, it can be considered negligible since it uses a hypertonic solution, which could easily be recycled [39]. However, accurate computation of all the energy and water demand factors should be clarified in future research (e.g., throughout life cycle assessment approach) to better understand the process's environmental impact. Moreover, the aforementioned "green" food pre-processing contributes to design a renewable natural product with valuable quality features through the reuse of industrial food waste.

Conclusions
The obtained results showed that OD pre-treatment applied alone resulted in a good retention of TPC and AA measured by the DPPH method, while PEF treatment alone showed a high content of vitamin C and AA assessed by the ABTS method. In both samples, the values were similar to those of control samples. However, the shortest drying times required for the OD and PEF pre-treated samples could have an additional positive effect on the sustainability of the overall process, by saving the energy required for the process. In general, an increased number of processing steps had a negative influence on the retention of the bioactive compounds and on the antiradical activity in all samples, while the color differences among them, although significant in certain cases, did not indicate a negative effect of any treatment.
In order to possibly scale-up the applied single/combined processing steps in a real industrial scenario, a further study is necessary considering both the energy and managing costs and the stability and nutritional quality of the obtained products, particularly in terms of environmental and economic sustainability.

Basic Mechanisms of the Pre-Treatments Applied
Drying is a technology widely used to obtain shelf-stable food products and snacks. However, drying is a time-and energy-consuming process, therefore the application of pre-treatments such as pulsed electric field (PEF) and osmotic dehydration (OD) alone or in combination could be important both for energy saving and preservation of the final food quality.
PEF technology consists in the application of pulses of high voltage and short duration (ms-µs-ns) to a biological material placed between two electrodes. The application of the external electric field (Ee) to the biological material promotes the electroporation of the tissue, which could be of different extents, reversible or irreversible, as it is shown in Figure 5. When the applied electric field strength (Ee) exceeds a critical value (Ee > Ec), a temporary membrane permeabilization takes place. However, if the electric field is still below the irreversible electroporation threshold, cells can recover their integrity and remain viable after the electric field exposure. The reversible electroporation (the created pores reseal immediately after removing the electrical field) could be used to incorporate different functional substances into the vegetal tissue, ensuring the survival of the electrically stimulated cells. On the other hand, when the electric field strength greatly exceeds Ec values (Ee >> Ec), a permanent membrane permeabilization takes place. This phenomenon leads to extensive leakage of the intracellular content and cell death, which find wild applications in the microbial inactivation of liquid foods and for the cell disintegration of biological tissues [40].
Electroporation and, consequently, cell disintegration of biological tissues upon the application of a low/moderate electric field strength (0.1-5 kV/cm) can be used for the enhancement of mass and heat-based processes of structure modification [41].
Osmotic dehydration is a partial dewatering impregnation process, usually carried out by immersion of a cellular tissue, which is characterized by semipermeable membranes, in hypertonic solution. Thanks to the differences in the water chemical potential between the tissue and the osmotic solution, an important mass transfer phenomenon can occur ( Figure 5). There is a water loss from the tissue into the osmotic solution, while at the same time, there is a simultaneous counter-diffusion of solutes from the concentrated solution into the tissue. During osmotic dehydration, the membrane responsible for osmotic transport progressively loses its selectivity, therefore a leakage of the natural solutes present in the cells (e.g., vitamins, pigments) can also occur [42].
Osmotic dehydration can be used as a pre-treatment for fruit and vegetables intended for further processing, like, for example, drying, contributing to the reduction in the drying time [43].
solution. Thanks to the differences in the water chemical potential between the tissue and the osmotic solution, an important mass transfer phenomenon can occur ( Figure 5). There is a water loss from the tissue into the osmotic solution, while at the same time, there is a simultaneous counter-diffusion of solutes from the concentrated solution into the tissue. During osmotic dehydration, the membrane responsible for osmotic transport progressively loses its selectivity, therefore a leakage of the natural solutes present in the cells (e.g., vitamins, pigments) can also occur [42]. Osmotic dehydration can be used as a pre-treatment for fruit and vegetables intended for further processing, like, for example, drying, contributing to the reduction in the drying time [43]. Funding: Support for this project was provided by Emilia-Romagna Region within the PSR 2014-2020 Project (Focus Area 5 C).

Conflicts of Interest:
The authors declare no conflict of interest.