A Comparison of Rice Straw and Whole-Crop Barley ( Hordeum vulgare L.) Silage Supplements on Performance and Carcass Characteristics of Hanwoo ( Bos taurus coreanae ) Steers

: This study aimed to analyze the e ﬀ ects of whole-crop barley silage (WCBS) and a high-concentrate diet combination to replace a rice straw / concentrate on growth performance, and meat quality of Hanwoo steers (HS). Twenty-four animals were allocated to two groups with a rice straw / concentrate control treatment (CON) and a WCBS / concentrate treatment group. The concentrate was o ﬀ ered to all animals based on live weight after a feeding trial of grass-based diets. Feed intake of WCBS was lower than the CON ( p < 0.01) and intake of concentrate was lower in the WCBS group than the CON in all experimental phases ( p < 0.01). WCBS increased backfat thickness (BFT) and slaughter weight (p > 0.05). The marbling score was slightly higher in the WCBS group ( p > 0.05). WCBS-fed beef had higher crude fat, lower crude protein, and moisture contents. WCBS feeding increased the levels of alpha-linolenic acid ( p < 0.01) and decreased arachidonic acid in meat ( p < 0.02). Hanwoo steers fed WCBS / concentrate improved feed intake, carcass traits, meat price, palatability, and FA content compared to those fed rice straw / concentrate, suggesting that WCBS is a potential source for improving the growth performance and meat quality in Hanwoo steers with signiﬁcant economic e ﬃ ciency. the means of replicates at nutrient compositions, BW, daily weight gain, feed intake, FCR, carcass traits, chemical composition, and fatty acid composition between two di ﬀ erent feeding trials. The analysis measured mean di ﬀ erences at 5% and 1% levels between groups using Duncan’s multiple range tests.


Introduction
Diet plays a primary role in altering the fatty acid (FA) composition in the body of pigs, sheep, and cattle [1]. Korean economic changes lead to increases in the demand for high-quality beef. Hanwoo is a major source of cattle meat with highly marbled beef production; it accounted for about 85% of slaughtered cattle in 2016 [2]. The demand for highly marbled beef has been raised; therefore, the use of grain-based diet when the fattening period has been increased in beef industries recently. Concentrate feed has excessive fermentable carbohydrate, which causes acidosis in beef cattle [3]. The replacement of plant-based materials in diet during the fattening period provides a significant impact on acidosis, as well as maintains the integrity and health of ruminal papillae and nutrient absorption. Nowadays, considerable quantities of concentrate are formulated with mixtures of grass or whole grain cereals, inter alia, to provide energy, protein, and essential nutrients to animals, and have

WCBS Preparation
Barley, cultivated at the National Institute of Animal Science (NIAS) of South Korea, was harvested at the ripening stage (Zadoks code 9) and was wilted under field conditions to obtain the moisture content of 70%. The wilted forage was inoculated (by spraying method) with L. plantarum (Chung-Mi Bio Co., Anseong, Korea) at a rate of 100 g/ton of forage. Simultaneously, the samples were wrapped with 16-18 layers of polyethylene film by round bale silage wrapping machine (Bio325, Vicon, Soest, Germany) and stored for 90 days at room temperature. Rice straw was obtained from a beef cattle farm in Kangwon Province, Korea, and was chopped into small pieces (4-5 cm lengths) and fed to animals in the control (CON) group.

Experimental Design and Animal Management
The experimental program was designed according to the Hanwoo care and user guidelines of the National Institute of Animal Science (NIAS/section no. 100), which is approved by the Internal Community Act (ICA) [21]. A total of 24 Hanwoo steers with a mean bodyweight of 206.67 ± 9.74 kg were obtained upon weaning (i.e., from 120 to 180 days of age). Animals were kept in individual cages at livestock farms located at Maam-myeon, Goseong-gun, and Gyeongsangnam-do, South Korea. Animals were individually penned and fed the experimental diets twice daily at 09:00 and 16:00 and the animals had free access to mineral blocks and water in troughs A total of 24 steers were allocated to two groups (Control and WCBS treated groups), each consisting of 12 animals. The mean initial body weight (BW) of steers were 212.08 kg for the CON and 201.25 kg for the WCBS treatment group (p > 0.05). Growth performance, feed intake, slaughter characteristics, meat quality, and fatty acid (FA) compositions of experimental meat were determined. The feed conversion ratio was determined by the following method; FCR = Feed intake/bodyweight gain.

Feeding System
All animals were fed a concentrated diet appropriate to each experimental period based on Korean Feeding Standards for Hanwoo [21]. Thus, crude protein (CP) and total digestible nutrients (TDN) levels of the concentrate were formulated by a commercial feed company in accordance with growth stages of Hanwoo steers (Table 1). After roughage or silage feeding, the concentrate was offered at each feeding time at 09:00 and 16:00. The feed intake was measured daily at 08:00 and 15:00, i.e., prior to the next feeding. The present study determined four growing phases, i.e., day 137 (initial feeding), day 124 (first fattening, after initial period), day 184 (mid fattening), day 295 (finish fattening), and day 740 (final feeding). Randomly collected samples of concentrate, rice straw and barley silage were taken monthly to determine the chemical composition [22]. The chemical compositions of concentrate were determined and published previously [23].

Analysis of Carcass Traits
To analyze carcass characteristics, samples of the muscle and the intermuscular adipose tissue surrounding the Musculus longissimus dorsi (M. longissimus dorsi) were collected after slaughter at a packing facility located at Eumseong in South Korea. Samples were stored at 4 • C for 48 h until further analysis. Intramuscular tissue of the M. longissimus dorsi was used to determine FA profiles. After 48 h post-slaughter chilling, a sample was removed from the left side of the carcass between the last rib and the first lumbar vertebra for evaluating back fat thickness (BFT) and rib-eye area (REA) traits. For the sensory evaluation, nine trained panelists recorded their preferences. After 30 min of blooming, the marbling score (1 = trace to 9 = very abundant), meat color (1 = very light cherry red to 7 = very dark red), fat color (1 = white to 7 = yellow), texture (1 = very fine to 9 = very coarse), and maturity (1 = very young to 9 = very old) were assessed, based on the Animal Products Grading Service [2]. Briefly, BFT was determined by measurements taken perpendicular to the outside surface, at a point two-thirds of the length of the rib-eye, between the last rib and the first lumbar vertebra [16]. The REA was analyzed at the surface of the cut using a standard grid. Approximately 10 g of chopped muscle was mixed with 10 mL deionized water to measure the pH using a pH meter (F-12, Horiba, Japan). Cooking loss was measured according to the procedure described [27], i.e., the percentage weight loss of meat samples (3 × 3 × 3 cm) after cooking in a hot water bath until the internal temperature reached 70 • C. Nine samples per treatment were cut into 1 cm 2 cross-sections with the fiber direction, 1.5 cm in length to measure the shear force (SF). The SF was measured by the texture analyzer (TA-XT2i, Stable Microsystems Ltd., Godalming, UK) equipped with a 25 kg load cell, a Warner-Bratzler shear blade, and a test speed setting of 2.0 mm/s, and was reported as the maximum force (kg).

Fatty Acid Composition Analysis
Lipids were extracted and homogenized with a chloroform/methanol solution using a homogenizer (PT-MR3100 Kinematica, Luzern, Switzerland) [28]. The chloroform was aspirated in the dry bath (Type 16500, USA) at 50 • C using nitrogen gas. Methylation of the lipids was analyzed prior to injection into the gas chromatograph (HP 5890 II fixed with a G1513A auto-sampler; Hewlett Packard Co., Alto Palo, CA, USA). The FA content was determined using a silica capillary column (100 m × 0.25 mm, i.d. × 0.20 µm thickness, Supelco, SP) with ultrapure helium as a carrier gas (flow rate = 1.0 mL/min). The temperature for the injector and detector was 250 • C. The initial temperature of the oven was 140 • C, and was then increased to 220 • C at a rate of 15 • C/min [29,30].

Statistical Analysis
The statistical analysis used the linear model procedures of SAS (SAS version 9.1 2002-2003, SAS Institute, Wake County, NC, USA) to report significant differences in the mean ± standard errors of the means of replicates at nutrient compositions, BW, daily weight gain, feed intake, FCR, carcass traits, chemical composition, and fatty acid composition between two different feeding trials. The analysis measured mean differences at 5% and 1% levels between groups using Duncan's multiple range tests.

Results and Discussion
In this study, the economic efficiency of replacing the rice straw with WCBS was determined in comparison with a control diet (using rice straw/concentrate), based on measurements of meat quality in Hanwoo steers. Table 2 Table 3 shows the mean values for growth performance and feed intake during the initial feeding, first-, mid-, and finish fattening period. Over the duration of the experimental periods, steers consumed 9975 kg of WCBS/concentrate (5775 kg WCBS and 4200 kg of concentrate) and 6836 kg of rice straw/concentrate (1736 of rice straw and 5100 kg concentrate). The CON group had a slightly higher initial mean body weight compared with WCBS, although the final mean body weights over all experimental periods were higher in the WCBS group compared to the CON (p > 0.05). The WCBS group had a higher daily body weight gain (0.98 kg) compared to the CON group (0.87 kg) in the initial feeding period (p < 0.05), whereas differences were not found in the fattening periods (p > 0.05). Nevertheless, intake of concentrate was lower in the WCBS group than in the CON in all experimental periods (p < 0.01). In the present study, it was noted that the total feed intake in DM basis was lower in WCBS than CON in all phases, although there was no significant difference in feed conversion ratio of WCBS group compared to CON. These results suggest that WCBS could provide an economically balanced concentrate replacer during all experimental periods, which is consistent with previous studies that show that high-quality forages improve performance and feed intake in steers [8,31]. The effects of WCBS on carcass yield and quality traits were determined and the data are shown in Table 4. The WCBS supplement significantly increased back fat thickness (BFT, p < 0.05). There are no significant changes, (p < 0.05) were reordered for slaughter weight and marbling score between control and experimental diet. However, the WCBS-based diet slightly increased the slaughter weight and marbling score more than the control diet. Besides, Rib-eye area (REA), dressing percentage, and quality traits (lean color, fat color, texture, maturity and quality grade) were almost too similar to control, but slight decreases were noted in the animal fed with WCBS. Previous studies involving different forage-to-concentrate ratios have demonstrated variable results with respect to carcass traits in fattening cattle. Higher subcutaneous and mesenteric adipose tissue weights in a high concentrate diet compared to a high roughage diet were due to the difference in the TDN intake among dietary treatments [32]. For carcass quality traits, the marbling score was slightly higher in the WCBS group (p > 0.05) and lean color in the WCBS group was a brighter red color (p = 0.08). The results of the present study based on a higher TDN intake in the WCBS group strongly agree with a previous study asserting that marbling tends to be positively associated with carcass weight and subcutaneous fat thickness [32,33]. Fat color tended to be a creamier white compared to the CON (p > 0.05). Barley sources in finishing diets produce lighter lean than maize grains, while fat color has been known to be determined by the concentration of carotenoids [33,34]. In addition, a previous study consistent with this result showed that up to 80% of carotenoids are destroyed in the ensiling process [8]. Consistent with the results of Murphy et al. [13], the current study indicates that a WCBS/concentrate combination was more feasible in improving carcass quality without the negative effect of carotenoids on fat color in beef cattle than by the intake of concentrate.
Thus, these studies suggest that WCBS can enhance the appearance for the consumer's choice of beef color, if they want a lighter meat, although texture, maturity, and quality grades were slightly higher in the CON (p > 0.05). The meat in the WCBS group had a lower moisture content and CP than that in the CON group, indicating that CF in the WCBS group was higher than that in the CON (p < 0.05; Table 5). These results concur with a previous study in which flavor desirability and flavor intensity increased with fat content but protein was not significantly different (p > 0.05), indicating that a higher fat content in WCBS steers tended to affect beef flavor in the sensory evaluation [35,36].
The results confirm that steers preferred eating combinations of WCBS/concentrate than the ration provided to the CON group  Table 6 presents the relative proportions of FAs between the WCBS and CON groups. The C18:3 n-3 concentrations increased in WCBS-fed steers compared to the CON (p < 0.01) and arachidonic acid (C20:4 n-6) decreased (p < 0.02). A previous study reported that grass-based diets resulted in higher levels of omega-3 within the lipid fraction of the meat, while omega-6 levels were left unchanged. As the concentration of grain increases in the grass-based diet, the concentration of n-3 FAs decreases in a linear fashion [8]. Taking these results into consideration, it might be assumed that grass-finished beef consistently produces a higher concentration of n-3 FAs (without affecting n-6 FA content), resulting in a more favorable omega-6:omega-3 ratio. Furthermore, n-3 FAs reportedly interfere with the synthesis of C20:4 n-6 from C18:2 n-6 via several mechanisms, such as the displacement of FA of the n-6 series [37]. Unsaturated treatments with linseed and fish oil decrease the C20:4 n-6 concentration in beef cattle [38]. These results demonstrate that WCBS was effective in providing unsaturated FA sources for fattening cattle and has the potential to produce beef with an abundance of PUFA for consumers.
WCBS feeding tended to increase palmitoleic acid, stearic acid, oleic acid, vaccenic acid, eicosanoic acid, and MUFA, and decrease myristic acid, palmitic acid, linoleic acid, SFA, and PUFA compared to the CON diet (p > 0.05). Previous studies found that decreasing the concentrate: forage ratio might increase palmitoleic acid in meat due to the presence of a higher palmitoleic acid content in forage [39]. Our results agree with a previous study that showed that grass finished meat contains elevated concentrations of stearic acid (C18:0), the only SFA with a net neutral impact on serum cholesterol, although there were no significant differences between groups. Grain-fed beef consistently produces higher concentrations of MUFAs when compared to grass fed beef, which includes FAs such as oleic acid (C18:1 cis-9), the primary MUFA in beef [8]. Grass-fed beef reportedly has a higher concentration of trans vaccenic acid (C18:1 t11), an important MUFA for the de novo synthesis of conjugated linoleic acid (CLA; C18:2 c-9, t-11), a potent anti-carcinogen that is synthesized within the body tissues [8]. The current study showed a similar trend to that of the previous study in terms of concentrate combinations with WCBS. Acidosis is related to the amount, frequency, and duration of grain feeding, due to the limit in buffering capacity in the rumen [40,41]. Grain feeding decreases the rumen bacterium Butyrivibrio fibrisolvens, the main rumen bacterium concerned with the biohydrogenation of linoleic acid and alpha linolenic acid, through a low ruminal pH [8,42]. From a significant increase in alpha linolenic acid, it was suggested that WCBS could improve ruminal metabolic disorders, such as low pH, by diminishing the usage of concentrate (pH data not shown). Several studies have attempted to improve the levels of these FAs for the benefit of the consumer [8,9,34,39]. As reported in a previous study, omega-3 FAs in meat products have a beneficial role in neonatal development and human health [43]; the current study, which substituted a proportion of the concentrate diet with WCBS, exhibited beneficial FA enhancement properties. WCBS feeding increased concentrations of palmitoleic acid, stearic acid, oleic acid, and alpha linolenic acid in muscle and adipose tissues. These results strongly indicate that beef industries need to re-evaluate feeding management to improve the efficiencies of feed usage as well as meat quality traits, which may also improve sustainability and competitiveness with food sources for humans in the agricultural marketplace. The present study demonstrated that Hanwoo steers fed WCBS had a high fat content similar to grass-fed animals, as was also suggested in a previous study [44].

Conclusions
The current study proves that the Hanwoo steers fed a WCBS/concentrate ration had improved feed intake, carcass traits, meat price, palatability, and FA content compared to those fed rice straw/concentrate. It suggests that WCBS is a valuable source of feed for improving the growth performance and meat quality in Hanwoo steers with substantial profitability compared to rice straw/concentrate rations.