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Article

Molecular Epidemiological Survey of Cryptosporidium in Ochotona curzoniae and Bos grunniens of Zoige County, Sichuan Province

by
Tian-Cai Tang
1,2,†,
Ri-Hong Jike
3,†,
Liang-Quan Zhu
4,
Chao-Xi Chen
1 and
Li-Li Hao
1,*
1
College of Animal Husbandry and Veterinary Medicine, Southwest Minzu University, Chengdu 610041, China
2
Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin 644000, China
3
Agricultural and Rural Bureau of Liangshan Yi Autonomous Prefecture of Sichuan Province, Liangshan 615000, China
4
China Institute of Veterinary Drug Control (IVDC), Beijing 100081, China
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Animals 2025, 15(14), 2140; https://doi.org/10.3390/ani15142140 (registering DOI)
Submission received: 19 June 2025 / Revised: 15 July 2025 / Accepted: 16 July 2025 / Published: 19 July 2025
(This article belongs to the Special Issue Coccidian Parasites: Epidemiology, Control and Prevention Strategies)
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Simple Summary

Cryptosporidium is an important zoonotic parasite that infects a wide range of animals, causing diarrhea, growth retardation, and economic losses in livestock. Although Cryptosporidium has been reported in some regions of Aba Prefecture, there is limited research on the prevalence and distribution of Cryptosporidium spp. in Ochotona curzoniae (plateau pika) and Bos grunniens (yak) in Zoige County. Therefore, the current study aimed to investigate the species and genotypes of Cryptosporidium in O. curzoniae and B. grunniens, and to reveal their potential zoonotic risk in Zoige County of Sichuan Province, China. The results that overall prevalence rate of Cryptosporidium in Zoige County was 8.3% (20/242), and three different Cryptosporidium species were involved, including C. bovis, C. ryanae, and an unidentified Cryptosporidium sp.; among them, an unidentified Cryptosporidium sp. was detected in O. curzoniae, and C. bovis and C. ryanae were detected in B. grunniens. These findings demonstrate that Cryptosporidium infections are present in both O. curzoniae and B. grunniens in Zoige County, with notable differences in infection rates and species composition, and provide valuable data on the epidemiology of Cryptosporidium in the region and offer scientific support for the healthy development of the livestock industry and public health management.

Abstract

In order to investigate the infection status of Cryptosporidium in O. curzoniae and B. grunniens in Zoige County, Sichuan Province, fecal samples from B. grunniens and gastrointestinal contents from captured O. curzoniae were collected between March and December 2023 from five townships (Dazhasi, Axi, Hongxing, Tangke, and Maixi). Genomic DNA was extracted, and nested PCR targeting the small subunit (SSU) rRNA gene of Cryptosporidium was performed. PCR-positive products were sequenced, trimmed, aligned, and subjected to phylogenetic analysis to determine species and genotypes. A total of 242 samples were obtained, of which 20 were Cryptosporidium SSU rRNA-positive, yielding an overall detection rate of 8.3% (20/242). The detection rates of O. curzoniae and B. grunniens were 7.0% (8/114) and 9.4% (12/128), respectively. Among the five sampling sites, Maixi town exhibited the highest detection rate (32.4%, 11/44), followed by Hongxing town (15.2%, 7/46) and Tangke town (4.6%, 2/44). Phylogenetic analysis detected an unidentified Cryptosporidium sp. in O. curzoniae, while C. bovis (n = 10) and C. ryanae (n = 2) were detected in B. grunniens. These findings demonstrate that Cryptosporidium infections are present in both O. curzoniae and B. grunniens in Zoige County, with notable differences in infection rates and species composition. Continued surveillance of Cryptosporidium in local livestock and wildlife is warranted to provide critical data for regional public health management.

1. Introduction

Cryptosporidium was first reported in 1907 [1] and is classified within the Apicomplexa (Phylum), Conoidasida (Class), Eucoccidiorida (Order), Cryptosporidiidae (Family), and Cryptosporidium (Genus) [2]. Its life cycle involves oocysts that excyst within the host’s gastrointestinal tract to release sporozoites, which develop through trophozoite–meront–gamont–zygote stages before forming thin- or thick-walled oocysts that are excreted in the feces [3,4]. To date, 49 valid species and over 120 genotypes of Cryptosporidium have been recognized, of which approximately 23 have been reported to infect humans, including C. hominis [5], C. meleagridis [6], C. canis, C. ubiquitum, C. cuniculus [7], C. felis [8], and C. viatorum [9,10,11]. Surveys indicate that human cryptosporidiosis cases have been documented in more than 90 countries and regions worldwide [12,13]. Cryptosporidium parasitizes the gastric or small-intestinal epithelial cells of a wide range of mammals, including livestock, humans, companion animals, and wildlife, and causes cryptosporidiosis, which is characterized by diarrhea in both humans and animals. In poultry and livestock, infection can lead to enteric and respiratory disease, manifesting as diarrhea, weight loss, and growth retardation, thereby posing a serious threat to animal husbandry; no fully effective drugs or vaccines for treatment or prevention currently exist. Vermeulen et al. used the GloWPa-Crypto L1 model to estimate global livestock-derived Cryptosporidium oocyst excretion, finding that approximately 3.2 × 1023 oocysts are shed annually through livestock feces, with cattle contributing the largest share, followed by chickens and pigs [14]. In China, over 24 provinces, autonomous regions, and municipalities have reported bovine cryptosporidiosis, with infection rates typically exceeding 10% in Shanxi [15], Jiangxi [16], Xinjiang [17], Ningxia [18], Yunnan [19], and Inner Mongolia [20]. Meta-analyses have demonstrated variation in infection prevalence among different cattle populations: Cai’s meta-analysis of dairy cattle in China reported an overall prevalence of 17.0% (3901/33,313) [21], while Geng’s meta-analysis of yak reported a prevalence of 10.52% (1192/8012) [22]. Additionally, infections in domestic dogs and cats are considered important zoonotic reservoirs for human cryptosporidiosis.
The plateau pika (Ochotona curzoniae (O. curzoniae)) is a small lagomorph in the family Ochotonidae, which is widely distributed across Tibet, Qinghai, southern Gansu, and northwestern Sichuan in China [19]. Despite its high reproductive capacity and abundance, reports of Cryptosporidium infection in O. curzoniae are extremely scarce; to date, only Zhang has documented such infections on the Qing–Tibetan Plateau [23]. Zoige County, located in northern Sichuan at the northeastern margin of the Tibetan Plateau, is a major pastoral region of northwestern Sichuan, encompassing some 10,620 km2 of grassland with an average altitude (3400–3700 m), annual temperature (0–2.0 °C), and annual rainfall (600–700 mm). Yak (Bos grunniens (B. grunniens)) represents the primary livestock resource and economic base for local herders. Both O. curzoniae and B. grunniens share the same grassland habitat and are herbivorous species. Due to their prolonged sympatric coexistence, Cryptosporidium infection in either species can readily spread to the other, establishing bidirectional transmission and enabling each to serve as a potential reservoir host. Furthermore, local herders’ traditional lifestyle and low awareness of personal hygiene substantially increase the risk of zoonotic disease transmission. However, no studies have assessed Cryptosporidium infection in O. curzoniae and B. grunniens in Zoige County. Thus, this study is the first to investigate their infection status, with the aim of providing data to inform control measures, support healthy livestock development, and enhance public health in the region.

2. Materials and Methods

2.1. Sample Collection

A total of 114 O. curzoniae were captured between March and December 2023, of these O. curzoniae, 24, 20, 20, 20, and 30 were captured from Dazhasi, Axi, Hongxing, Maixi, and Tangke, respectively. O. curzoniaes were captured using mouse snap traps. Then, to avoid cross-contamination, the intestinal (colon) and gastric contents from each O. curzoniae were collected separately using sterile plastic gloves, stored in liquid nitrogen, and transported to the laboratory and stored at −80 °C. The body of each O. curzoniae was deeply buried to avoid being eaten by dogs, cats, or other wild carnivores. When determining the results, if either intestinal (colon) or gastric content tested positive, then O. curzoniae was determined as positive. In the same time period, a total of 128 fresh fecal samples from B. grunniens (2–3 years old) were collected from Dazhasi (n = 18), Axi (n = 40), Hongxing (n = 26), Maixi (n = 14), and Tangke (n = 14), located between 102°08′–103°39′ E longitude and 32°56′–34°19′ N latitude.

2.2. DNA Extraction

Approximately 150 mg of intestinal and gastric contents of O. curzoniae and 200 mg fecal samples of B. grunniens were added to ddH2O, and homogenates were centrifuged for 5 min at 5000× g. The total DNA of all samples was extracted using the TIANamp Genomic DNA Kit (TIANGEN Biotech Co., Ltd., Beijing, China, Cat. No.GDP304, https://en.tiangen.com/ (accessed on 20 December 2023)) according to the manufacturer’s instructions. After each extraction procedure, to assess the quality of the DNA, the DNA concentration was quantified using NanoDrop 2000 (Thermo Scientific, Wilmington, DE, USA), with samples demonstrating concentrations > 30 ng/μL (A260) being selected for subsequent experiments. The extracted genomic DNA was stored in a freezer at −20 °C until PCR amplification.

2.3. PCR Amplification

Cryptosporidium spp. in the samples was confirmed by nested PCR amplification of the SSU rRNA gene according to the previous report [24,25]. A PCR product of approximately 760 bp was first amplified with primers CrF1: 5′-GACATATCATTCAAGTTTCTGACC-3′ and CrR1: 5′-CTGAAGGAGTAAGGAACAACC-3′. A secondary PCR product of about 580 bp was then amplified with primers CrF2: 5′-CCTATCAGCTTTAGACGGTAGG-3′ and CrR2: 5′-TCTAAGAATTTCACCTCTGACTG-3′ [24,25]. The amplification reaction was conducted in a 25 µL volume containing 1.0 µL template DNA (10 U µL−1), 12.5 µL 2× PCR mix (TransGen Biotech Co., Ltd., Beijing, China, Cat. No. AS111), 2.0 µL dNTPs, and 9.5 µL distilled water. Each PCR included a positive control (DNA of C. bovis preserved in the laboratory) and a negative control (nuclease-free water). The PCR cycling conditions for the SSU rRNA gene of Cryptosporidium spp. comprised 98 °C for 2 min, followed by 35 cycles of 98 °C for 10 s, 57 °C for 10 s, and 72 °C for 6 s, and then a final extension at 72 °C for 2 min. The PCR products (3 μL) were electrophoresed at 100 V for 25 min on a 1.3% agarose gel, and the results were observed in a gelimager (Tanon Science & Technology Co., Ltd., Shanghai, China, Cat. No. Tanon 1600).

2.4. Sequence Analysis and Phylogenetic Tree

The final positive PCR products were subjected to Sanger bidirectional sequencing by Sangon Biotech Company (Chengdu, China) using the second pair of primers. Firstly, all the obtained sequences were analyzed and manually edited by employing DNA Star (https://www.dnastar.com/ (accessed on 12 January 2024)) and were subjected to a nucleotide BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi, accessed on 15 January 2024) search through the NCBI database. Subsequently, the sequences with the highest similarity to the blast results were selected (1–4 sequences), and the SSU rRNA gene sequences of major Cryptosporidium spp., including C. ryanae, C. bovis, C. hominis, C. meleagridis, and C. felis reported in China, were selected as reference sequences to construct a phylogenetic tree. Lastly, the phylogenetic tree was constructed based on the Neighbor-Joining (NJ) method using MEGA 11.0 (https://www.megasoftware.net/, accessed on 5 March 2024), and 1000 replicates (bootstrap value) were selected to assess the robustness of the findings.

2.5. Statistical Analysis

Firstly, the Pearson Chi-square test with the software SPSS 19.0 (IMB Corporation, Armonk, NY, USA) was used to assess whether there was a significant difference in Cryptosporidium spp. prevalence between different sampling locations, O. curzoniae, and B. grunniens. A p-value of <0.05 was considered significant. Secondly, the specimen numbers analyzed in the present study are relatively low; therefore, the statistical outcomes obtained were reanalyzed with Fisher’s exact test and confirmed [26]. In addition, the strength of the association between prevalence and test conditions was assessed by calculating the odds ratio (ORs) and 95% confidence interval (95% CI) [27].

3. Results

3.1. Occurrence of Cryptosporidium spp.

The analysis revealed that among the 242 hosts, 20 were positive for Cryptosporidium, with an overall prevalence of 8.3%. The infection rates of O. curzoniae and B. grunniens were 7.0% (8/114) and 9.4% (12/128), respectively (Table 1). There were no significant differences in the prevalence of Cryptosporidium spp. between the O. curzoniae and B. grunniens (χ2 = 0.441, p = 0.506). In our study, we identified three Cryptosporidium species: Cryptosporidium sp. (n = 8), C. bovis (n = 10), and C. ryanae (n = 2). In O. curzoniae, only one positive sample was detected in gastric contents, while all other positives were found in intestinal contents. Three novel Cryptosporidium spp. genotypes were identified, all exclusively detected in Maixi town, with no occurrences observed in the other four surveyed townships. Among the surveyed townships, Cryptosporidium sp. infection rates in B. grunniens were highest in Hongxing town (27.9%, 7/26), followed by Maixi town (21.4%, 3/14) and Tangke town (14.3%, 2/14). there were no obvious differences in the prevalence of Cryptosporidium spp. among the three regions from B. grunniens (χ2 = 0.66, p = 0.72). For location, Maixi town (32.4%, 11/44) had a significantly higher infection rate than Tangke town (4.6%, 2/44), with statistical significance (χ2 = 8.12, p = 0.004). However, no statistically significant difference was detected between Hongxing town (15.2%, 7/46) and Maixi town and Tangke town. C. ryanae and C. bovis were identified only in B. grunniens; among them, C. ryanae (n = 2) was detected only in Hongxing town, while C. bovis was detected in Hongxing town (n = 5), Maixi town (n = 3), and Tangke town (n = 2).

3.2. Phylogenetic Analysis Based on SSU rRNA Gene of Cryptosporidium

All PCR products of the SSU rRNA genes for Cryptosporidium spp. were sequenced and compared to each other. A total of five unique sequences were obtained and submitted to GenBank; among these, three sequences from O. curzoniae and two from B. grunniens were named Cryptosporidium sp. Z-OC1 (PP463757), Cryptosporidium sp. Z-OC2 (PP463758), Cryptosporidium sp. Z-OC3 (PP463759), C. bovis Z-Y1 (PV536157), and C. ryanae Z-Y2 (PV536158). As shown in Figure 1, Cryptosporidium sp. Z-OC1 (PP463757) was clustered with the Cryptosporidium sp. isolate from Mongolian pika genotype (OR565220), with the closest genetic relationship and a similarity of 98.3%. As for Cryptosporidium sp., Z-OC2 (PP463758) and Cryptosporidium sp. Z-OC3 (PP463759) were clustered with the Cryptosporidium sp. (KF971356) isolate from B. grunniens in Qinghai Province, China, exhibiting 99.17% and 94.60% genetic similarity, respectively; C. bovis Z-Y1 (PV536157) was clustered with the C. bovis isolate DF1 (PP335809) from B. grunniens in Daofu County of Sichuan Province, with 100% similarity; C. ryanae Z-Y2 was clustered with the C. ryanae (KJ020910) from calves in Shaanxi Province, with 100% similarity.

4. Discussion

This is the first report of the detection of Cryptosporidium spp. in O. curzoniae and B. grunniens in Zoige County of Sichuan Province, with occurrences of 7.0% (8/114) and 9.4% (12/128), respectively. Among the eight SSU rRNA-positive O. curzoniae samples, only one was amplified from gastric contents-designated Cryptosporidium sp. Z-OC3 (GenBank accession no. PP463759), whereas the remaining positives originated from intestinal contents. This may be related to the internal environment of the stomach, where a large amount of gastric acid is secreted, which can inactivate most oocysts. Therefore, there are relatively few Cryptosporidium species that can parasitize the stomach, mainly C. muris and C. andersoni [28]. In addition, a study on the infectivity of Cryptosporidium in the gastrointestinal tract of Mongolian gerbils showed that there is cross-immunity among gastric Cryptosporidium species, but not between intestinal and gastric isolates [29]. Research on Cryptosporidium in O. curzoniae is exceedingly limited: to date, Zhang (2018) reported a 6.3% (4/64) prevalence in Qinghai O. curzoniae, identifying two genotypes–one novel and C. parvum [23]. In contrast, lagomorphs more broadly have been studied, such as a meta-analysis by Chen (1951–2024) found a global prevalence of 6.1% in lagomorphs (domestic rabbits, wild hares, and pikas), with pika infection at 17.1% [30]; another meta-analysis of Chinese lagomorphs (1981–2023) reported an overall prevalence of 3.9%, with pika infection at 6.2% [31], suggesting that members of Ochotonidae are particularly susceptible. Our detection rate of 7.0% (8/114) in Zoige County O. curzoniae aligns closely with these earlier findings. Notably, all O. curzoniae positives in our study derived from Maixi town—the only site with alternating meadow and sandy soils, severe northern desertification, and fragmented grassland—where higher host density likely elevates transmission risk. Additionally, the relatively small sample size may also contribute. Future investigations should include additional herbivores and larger sample numbers to map Cryptosporidium distribution across Zoige.
In this study, B. grunniens exhibited a 9.4% (12/128) detection rate, lower than reports from Qinghai Province in 2013, 2014, and 2016—24.2% (142/586), 30.0% (98/327), and 28.5% (158/554), respectively [32,33,34], and lower than our laboratory’s earlier finding of 14.8% (32/216) in B. grunniens from Hongyuan County, Sichuan [35]. Conversely, our detection rate exceeds those reported in Naqu Prefecture, Tibet (9.6%, 105/1100), and for white yaks in Gansu (5.3%, 4/76) [36,37]. Collectively, these data illustrate regional variation in B. grunniens Cryptosporidium prevalence. Additionally, the number of samples, age distribution of samples, grazing method, and sample sizes could be the contributing factors. Cattle are recognized as the most common mammalian host for Cryptosporidium, with pre-weaned calves being the principal reservoir. Across 24 Chinese provinces, bovine prevalence is estimated at 14.5% (5265/36,316), rising to 45.8% (141/308) in diarrheic calves [38]. Meta-analyses of various Chinese hosts yield descending overall prevalence of dairy cattle (17.0%; 3901/33,313) [21], swine (12.2%; 4349/30,404) [39], yaks (10.5%; 1192/8012) [22], sheep and goats (4.9%) [40], and lagomorphs (3.9%) [18]. A Nigerian meta-analysis similarly ranked cattle highest at 26.1%, followed by goats (26.0%), pigs (20.0%), sheep (16.6%), humans (15.0%), laboratory animals (9.0%), and birds (7.2%) [41]. These findings underscore the need for enhanced monitoring of Cryptosporidium in bovids across the Qinghai–Tibetan Plateau.
Phylogenetic analysis of SSU rRNA sequences in this study identified three taxa, Cryptosporidium sp., C. bovis, and C. ryanae, consistent with previous Sichuan reports [33,42]. Cryptosporidium sp. was recovered from O. curzoniae, whereas C. bovis and C. ryanae were detected in B. grunniens. To date, B. grunniens have been found to harbor C. ryanae, C. bovis, C. baileyi, C. andersoni, C. suis-like, C. parvum, C. hominis, C. canis, C. struthionis, C. ubiquitum, C. xiaoi, C. suis, and several novel genotypes [22,32,33,34,35,36,37,43], with C. ryanae, C. bovis, C. andersoni, and C. parvum being most common in bovines [44,45]. C. bovis was first reported from cattle feces in 2005 by Fayer et al., with a prevalent low pathogenic species mainly infecting post-weaned and adult cattle [46]. It was first reported in Indian dairy farm workers in 2010 [47], and subsequently in Australian farm workers [48], Egyptian children with diarrhea [49], and Colombian individuals [50], indicating zoonotic potential. C. ryanae, first detected in cattle in 2008 [51], is a common, low-pathogenic, or asymptomatic bovine species capable of infecting cattle of all ages, with no confirmed human cases to date. Currently, C. bovis has been detected in sheep or goats from China [52], the United States [53], Poland [54], Iran [55], and Tunisia [56], and was first identified in camels on the Qinghai–Tibet Plateau in 2020 [57], but has not yet been reported in other animals. In contrast, C. ryanae has been identified in horses [58], red deer [59], Tibetan sheep [52], domestic cats [60], marsh deer [61], and brown rats [62], indicating that both C. bovis and C. ryanae have potential for cross-species transmission. Among them, C. bovis is mainly transmitted between cattle and sheep, whereas C. ryanae may have a broader host range for cross-species transmission.
In our study, the species of Cryptosporidium detected in O. curzoniae and B. grunniens in Maixi town differed, with a finding similar to those of related studies on the Qinghai–Tibetan Plateau [23,33,63], and likely related to the host specificity of Cryptosporidium infections. Research shows that cattle are mainly infected with C. parvum, C. ryanae, C. andersoni, and C. bovis [21]; rodents with C. muris, C. tyzzeri, and C. viatorum [64]; sheep with C. parvum, C. xiaoi, C. ubiquitum, and C. ovis [65]; and lagomorphs with C. cuniculus [30,31]. For example, on the same farm in Qinghai Province, B. grunniens were found to harbor C. andersoni, C. bovis, C. ryanae cattle type, C. ryanae buffalo type, and C. suis-like, whereas Tibetan sheep carried C. xiaoi and C. ubiquitum [33]. In cattle on the Qinghai–Tibetan Plateau, C. andersoni, C. canis, C. bovis, C. hominis, C. struthionis, C. ryanae, and C. serpentis have been detected, while in sheep, C. parvum and C. canis have been reported [63]. In Qinghai field mice, C. parvum, C. ubiquitum, C. canis, and a novel genotype were identified, whereas in O. curzoniae, only C. parvum and a novel genotype were found [23]. According to existing studies and the phylogenetic tree constructed in this study, the possibility of transmission of C. ryanae and C. bovis between B. grunniens and O. curzoniae appears to be very low. However, reports on Cryptosporidium infection in O. curzoniae are currently scarce, and it remains unclear whether other Cryptosporidium species (e.g., C. cuniculus, C. parvum, and C. ubiquitum) can be transmitted among O. curzoniae, B. grunniens, and humans. Therefore, continuous surveillance of Cryptosporidium infection in humans, domestic animals, and wildlife in Zoige County is still necessary.

5. Conclusions

In summary, for the first time, this study documents Cryptosporidium infection in O. curzoniae and B. grunniens in Zoige County, revealing differences in prevalence and species composition. Given the paucity of epidemiological data on O. curzoniae and incomplete knowledge of circulating Cryptosporidium species and genotypes, comprehensive investigations are warranted to assess the parasite’s distribution among other livestock and wildlife hosts in the region in the future.

Author Contributions

Conceptualization, T.-C.T. and L.-L.H.; methodology, T.-C.T. and L.-Q.Z.; software, L.-Q.Z. and C.-X.C.; validation, R.-H.J.; formal analysis, R.-H.J. and L.-Q.Z.; investigation, R.-H.J.; resources, R.-H.J.; data curation, T.-C.T. and R.-H.J.; writing—original draft, T.-C.T. and L.-L.H.; writing—review and editing, T.-C.T., C.-X.C., and L.-L.H.; visualization, L.-Q.Z.; supervision, C.-X.C.; project administration, T.-C.T. and L.-L.H.; funding acquisition, T.-C.T. and L.-L.H. All authors have read and agreed to the published version of the manuscript.

Funding

The authors declare that financial support was received for the research, authorship, and/or publication of this article. This research was financially supported by the National Key Research and Development Program of China (2023YFD1801302), Southwest Minzu University Double World-Class Project (XM2023014), and Teacher Training Project of Yibin University (412-2021PY066).

Institutional Review Board Statement

The study was conducted in accordance with the Declaration of Helsinki and approved by the Animal Ethics Committee of Southwest Minzhu University Plateau (protocol code SMU-2023-026 and 26 February 2023); O. curzoniae were collected and inspected by qualified veterinary officers. In this study, no experiment was conducted on live animals.

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

The sequences generated in this study were submitted to GenBank. The names of the repository/repositories and accession number(s) can be found below: https://www.ncbi.nlm.nih.gov/genbank/ (accessed on 18 April 2025), PP463757, PP463758, PP463759, PV536157, and PV536158.

Acknowledgments

We thank Chen-Dong Xiao, Yao Pan, Ke-Lei Zhou, and Ying-Lin Li for their help with the validation of the results/experiments; Hong-Xi Chen for help with investigation and supervision; and Qu-Wu Jise for help with formal analysis. We are grateful to the reviewers for their invaluable comments to improve this manuscript.

Conflicts of Interest

The authors declare no conflicts of interest.

Abbreviations

The following abbreviations are used in this manuscript:
SSU rRNASmall Subunit Ribosomal RNA
NCBINational Center for Biotechnology Information
PCRPolymerase Chain Reaction
NJNeighbor-Joining

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Animals 15 02140 g001
Figure 1. Phylogenetic tree based on Cryptosporidium SSU rRNA gene. ●: Sequence obtained from O. curzoniae. ▲: Sequence obtained from B. grunniens. Bootstrap values from 1000 pseudoreplicates are indicated at the left of the supported node. Scale bar indicates an evolutionary distance of 0.05 substitutions per site in the sequence.
Figure 1. Phylogenetic tree based on Cryptosporidium SSU rRNA gene. ●: Sequence obtained from O. curzoniae. ▲: Sequence obtained from B. grunniens. Bootstrap values from 1000 pseudoreplicates are indicated at the left of the supported node. Scale bar indicates an evolutionary distance of 0.05 substitutions per site in the sequence.
Animals 15 02140 g001
Table 1. The infection rates of Cryptosporidium in O. curzoniae and B. grunniens in Zoige County of Sichuan Province.
Table 1. The infection rates of Cryptosporidium in O. curzoniae and B. grunniens in Zoige County of Sichuan Province.
LocationHostNo. Positive/No. of SamplesPrevalence% (95% CI)OR (95% CI)Cryptosporidium spp.Location Subtotal % (95% CI), OR
DazhasiO. curzoniae0/240
B. grunniens0/180
AxiO. curzoniae0/200
B. grunniens0/400
HongxingO. curzoniae0/200 15.2 (6.6–28.0), 3.8
B. grunniens7/2627.9 (12.3–47.0) C. ryanae (n = 2),
C. bovis (n = 5)
MaixiO. curzoniae8/2040.0 (19.1–63.9)2.4 (0.5–11.6)Cryptosporidium sp. (n = 8)32.4 (17.2–51.2), 10.0
B. grunniens3/1421.4 (4.8–46.6)ReferenceC. bovis (n = 3)
TangkeO. curzoniae0/300 4.6% (0.6–14.8),
Reference
B. grunniens2/1414.3 (1.8–37.8) C. bovis (n = 2)
Host subtotalO. curzoniae8/1147.0 (2.3–11.7)Reference
B. grunniens12/1289.4 (4.9–15.7)1.4 (0.5–3.5)
Total 20/2428.3 (5.1–12.5)
Reference: The reference group used for calculating the odds ratio (OR). The OR value for this group is fixed at 1.0. OR values for other groups represent the risk ratio relative to this group. Reference group for OR calculation, adjusted for location and host. OR: Odds Ratio. CI: Confidence Interval.
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Tang, T.-C.; Jike, R.-H.; Zhu, L.-Q.; Chen, C.-X.; Hao, L.-L. Molecular Epidemiological Survey of Cryptosporidium in Ochotona curzoniae and Bos grunniens of Zoige County, Sichuan Province. Animals 2025, 15, 2140. https://doi.org/10.3390/ani15142140

AMA Style

Tang T-C, Jike R-H, Zhu L-Q, Chen C-X, Hao L-L. Molecular Epidemiological Survey of Cryptosporidium in Ochotona curzoniae and Bos grunniens of Zoige County, Sichuan Province. Animals. 2025; 15(14):2140. https://doi.org/10.3390/ani15142140

Chicago/Turabian Style

Tang, Tian-Cai, Ri-Hong Jike, Liang-Quan Zhu, Chao-Xi Chen, and Li-Li Hao. 2025. "Molecular Epidemiological Survey of Cryptosporidium in Ochotona curzoniae and Bos grunniens of Zoige County, Sichuan Province" Animals 15, no. 14: 2140. https://doi.org/10.3390/ani15142140

APA Style

Tang, T.-C., Jike, R.-H., Zhu, L.-Q., Chen, C.-X., & Hao, L.-L. (2025). Molecular Epidemiological Survey of Cryptosporidium in Ochotona curzoniae and Bos grunniens of Zoige County, Sichuan Province. Animals, 15(14), 2140. https://doi.org/10.3390/ani15142140

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