Effects of Perilla Seed Meal on Productive Performance, Egg Quality, Antioxidant Capacity and Hepatic Lipid Metabolism of Wenchang Breeder Hens

Simple Summary Under modern intensive farming conditions, high-intensity production increases liver stress in laying hens. Liver damage also leads to decreased productivity and increased mortality in laying hens. As a byproduct of perilla seed oil, perilla seed meal has a certain anti-stress effect. In this experiment, a small amount of perilla seed meal was added to the diet. The results showed that perilla seed meal had a positive impact on antioxidant capacity, liver lipid metabolism and egg quality. Abstract The aim of this study was to investigate the effects of adding perilla seed meal (PSM) to the diet on reproductive performance, egg quality, yolk fatty acids, antioxidant capacity and liver lipid metabolism in breeding hens. A total of 192 31-week-old yellow-feathered hens were randomly divided into 4 treatments with 6 replicates of 8 birds for 8 weeks. The chickens were fed a typical corn–soybean meal diet containing 0% (control), 0.3%, 0.6%, and 1% PSM. The results showed that PSM can change the productivity of laying hens. Adding 0.6% PSM to the feed reduced the mortality rate of chickens. Adding 1% PSM improved the fertilization rate and hatching rate of chickens. Regarding egg quality, the albumen height and Haugh unit were improved in the 0.6% PSM group. The content of MUFAs and PUFAs in the egg yolk was increased in all the PSM groups, while SFAs were only increased in the 0.6% PSM group. Among the indicators related to lipid metabolism, serum GLU decreased in all the PSM groups. The 0.6% PSM group had a reduction in serum and liver TG, as well as reductions in serum LDL-C and ALT. The same results were observed for the abdominal fat percentage in the 0.6% PSM group. Liver lipid metabolism-associated gene expression of FAS and LXRα was decreased in all the PSM groups, and the mRNA expression of ACC and SREBP-1c was significantly reduced in the 0.6% PSM group. HE staining showed that the vacuoles in the liver tissue gradually decreased with increasing PSM doses, especially the 1% PSM dose. Lipid droplets with a similar trend were observed using Oil Red O staining. In the results of the antioxidant capacity test, the serum T-AOC was increased in the 0.6% and 1% PSM groups, and the SOD in both the serum and liver was significantly increased in all the PSM groups. The expression of antioxidant-related genes such as Nrf2, NQO-1, HO-1, CAT and GSH-Px was significantly upregulated in the 1% PSM group. In conclusion, the PSM diet improved the lipid metabolism and antioxidant capacity of breeding hens. PSM reduces mortality and improves fertilization and hatchability in the late laying period of chickens, resulting in greater benefits. We recommend adding 0.6% PSM to layer feed, which improves the physical condition of the hens and brings higher economic benefits.


Introduction
Under modern intensive farming conditions, breeder hens are prone to inflammation, fat deposition, autophagy and oxidative stress in the liver [1].The influence of nutrition, stress and other factors in the late laying period can alter the homeostasis of liver fat, affect lipid metabolism and easily cause fatty liver bleeding syndrome [2].
The liver is a major site of lipid biosynthesis and has an important function in controlling systemic glucose and lipid metabolism [3].It also supports nutrient absorption and utilization [4].The liver is also the site of the synthesis of lipids and proteins necessary for yolk production [5].As chickens age, lipid accumulation gradually leads to oxidative stress damage, resulting in reduced antioxidant capacity, impaired liver function and hepatic steatosis [6][7][8].Due to the diverse functions and vital importance of the liver, any reduction in its function can lead to metabolic disorders and a deterioration in the quality of egg production [9].Many factors can cause metabolic diseases in breeder hens, but nutritional factors have been key to the etiology of hapatic lipid metabolism [10].
Perilla (Perilla frutescens L.) is an annual plant in the Labiatae family and is widely cultivated as an important crop in East Asian countries [11].Perilla seeds (PS) contain numerous active ingredients with different effects, including anti-allergic, antibacterial, anti-inflammatory, hypolipidemic, antioxidant and anticancer properties [12].In addition, PS effectively prevents cell aging [13].Perilla seed meal (PSM) is a byproduct of PS oil extraction.PSM is a nutrient-rich substance containing large amounts of proteins (35 to 45%), fiber (55 to 65%), and small amounts of polysaccharides, polyols, fatty acids, phytosterols, flavonoids and phenolic compounds [14,15].A growing body of research suggests that plant products such as flavonoids and polyphenols play a crucial role in performing antioxidant and anti-inflammatory functions in animals [16][17][18].
Wenchang chicken features heat resistance and delicious meat quality [19].However, it should be noted that the Wenchang chicken is a breed of chicken native in China with relatively low egg production [20].The peak laying age of Wenchang chickens is 23 weeks old [21].The egg production rate of Wenchang chickens peaks in the 7th or 8th postpartum week, and more than 60% of the egg production rate can be maintained for 15-18 weeks [22].Compared to research on the meat quality of Wenchang chickens, there are few studies on their production performance.
Previous studies have found that perilla seed extract (PSE) lowers blood lipids in mice and breeder hens and affects egg quality and fatty acid composition [23][24][25].PS consumption by the hens could also influence the unsaturated fatty acid content of the eggs and thus improve their nutrition [26,27].Since PSM is rich in high-quality fats and nutrients, these experiments examined whether PSM can affect liver lipid metabolism and antioxidant capacity in breeder hens, as well as the effects of PSM on laying performance, egg quality and fatty acid content of the eggs.The aim of this study was to create a theoretical basis for the application of PSM in egg production.

Preparation of Perilla Seed Meal
The PS was manufactured in Weiyuan Town, Changshun County, Qiannan Buyi Miao Autonomou Prefecture, Guizhou Province.PSM was the residual product after pressing the perilla seeds for oil production and roasting them at 120 • C for 30 min in a microcomputer roaster.The nutritional ingredients and active ingredients of the PSM are listed in Table S1.The nutritious ingredients of PSM have been published in a previous article [28].The total polysaccharides of the PSM were determined using the phenol-sulfuric acid method with glucose as a standard.The total polysaccharide content of the PSM was measured to be 1.33%.The determination of the total flavonoids and total phenolics was was measured to be 1.33%.The determination of the total flavonoids and total pheno was carried out using colorimetric kits (Jiancheng Bioengineering Institute, Nanji China).The total flavonoid and total phenolic contents were 1.32% and 0.44%, respectiv The rosemarinic acid content was measured using Waters Breeze QS high-performa liquid chromatography (HPLC), and the results are shown in Figure 1.The amoun rosmarinic acid was calculated to be 0.19%.

Animals, Diet and Experimental Design
A total of 192 Wenchang hens (31 weeks old, 1.65 ± 0.20 kg body weight) with sim laying rates were obtained.All experimental protocols were approved by the Animal C and Use Committee of the South China Agricultural University (approval number: SY 2019-0136, Guangzhou, China).
The hens were randomly divided into 4 groups (each group has 6 replicates wit hens in one replicate) and subjected to the following feeding strategies: (1) control gro basal diet; (2) PSM-L group, basal diet + 3 g/kg PSM; (3) PSM-M group, basal diet + 6 g PSM and (4) PSM-H group, basal diet + 10 g/kg PSM.The photoperiod was set at 16 L: throughout the study.The chickens were housed in three-tiered cages (45 × 40 × 40 two chickens per cage), and they received 85 g of feed per bird per day to prevent ov feeding and had access to fresh water ad libitum.The breeder hens received artificial semination every 3 days with 35 μL of pooled semen per bird, as described by Liu [ The ingredients and nutrient composition of the basic diets are shown in Table 1.

Animals, Diet and Experimental Design
A total of 192 Wenchang hens (31 weeks old, 1.65 ± 0.20 kg body weight) with similar laying rates were obtained.All experimental protocols were approved by the Animal Care and Use Committee of the South China Agricultural University (approval number: SYXK 2019-0136, Guangzhou, China).
The hens were randomly divided into 4 groups (each group has 6 replicates with 8 hens in one replicate) and subjected to the following feeding strategies: (1) control group, basal diet; (2) PSM-L group, basal diet + 3 g/kg PSM; (3) PSM-M group, basal diet + 6 g/kg PSM and (4) PSM-H group, basal diet + 10 g/kg PSM.The photoperiod was set at 16 L:8 D throughout the study.The chickens were housed in three-tiered cages (45 × 40 × 40 cm, two chickens per cage), and they received 85 g of feed per bird per day to prevent overfeeding and had access to fresh water ad libitum.The breeder hens received artificial insemination every 3 days with 35 µL of pooled semen per bird, as described by Liu [29].The ingredients and nutrient composition of the basic diets are shown in Table 1.  2 The nutrient levels were calculated using data provided by the Feed Database in China. 3Digestible energy values were calculated based on the feed composition (DM basis), whereas the others were measured values.

Productive Performance and Egg Quality
Regarding the productive performance, the daily egg production, egg weight, number of abnormal eggs (broken, small, large or shell-less eggs) and number of dead chickens were recorded for each replicate during the experimental period.Egg production records were used to calculate the average egg weight, laying rate, abnormal egg rate and mortality rate.
To evaluate egg quality, 24 qualified eggs from each group (4 eggs/replication × 6 replicates/group) were collected after 56 days of the feeding experiment and used for egg quality analysis.The weight of the egg, yolk, white and eggshell was measured using an electronic scale.The long/short diameter and eggshell thickness of the eggs were measured using a vernier caliper.The eggshell strength and protein height were determined using an eggshell strength tester (RH-DQ200, Runhu International Co., Ltd., Guangzhou, China).The egg yolk color was determined using an Egg Analyzer (EA-01, Orka Technology Ltd., Ramat Hasharon, Israel).
To evaluate the hatching performance, during the 8th week of the experiment, eggs were collected on 4 consecutive days, and 3 eggs were selected per day from each replicate.The eggs were stored in the same incubator (Bengbu Sanyuan Incubation Equipment Co., Ltd., Anhui, China) and incubated at 37.2 • C to 38.0 • C and 60 to 75% relative humidity.Unfertilized hatching eggs and dead embryonic eggs were identified using egg illumination on the 13th day of incubation.At the end of incubation on the day 21, the number of dead eggs was counted, and the number of chicks was recorded.The fertilization rate, dead embryo rate and hatching rate of the hatched eggs were counted in each replicate.

Measurements of the Fatty Acid Content of Egg Yolks
At the end of the experiment, 12 eggs per group were collected (2 eggs/replication × 6 replicates/group) to measure the fatty acid content of the yolks using a Shimadzu GC-2010 PRO gas chromatograph.The mean content of each fatty acid was used to calculate the total saturated fatty acids (SFA), total monounsaturated fatty acids (MUFA) and total PUFA.The fatty acid measurement services were provided by Waltek Testing Group (Foshan, China) Co., Ltd.

Collection of Blood and Tissue Samples
At the end of the feeding period, six hens (one hen per replicate) were randomly selected and fasted for 12 h for sampling.The blood samples were collected from the wing vein into tubes, and the tubes were left at room temperature for 20 min and then centrifuged at 4 • C for 10 min at 3000× g to collected the serum.The serum samples were stored at −20 • C for subsequent analysis.The chickens were euthanized by exsanguination, and the liver and middle jejunum were immediately removed and quickly frozen at −80 • C for further analysis.

Biochemical Variables and Antioxidant Capacity of Plasma and Liver
The serum glucose (GLU), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels were determined using an automatic analyzer (COBUS MIRA Plus, Roche Diagnostic System Inc., Rotkreuz, Switzerland) according to the manufacturer's guidelines.The plasma concentrations of total cholesterol (TC), triglyceride (TG), lowdensity lipoproteins (LDL), malondialdehyde (MDA), superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and glutathione (GSH) were analyzed using colorimetric kits (Jiancheng Bioengineering Institute, Nanjing, China).The plasma concentrations of follicle-stimulating hormone (FSH) and estradiol (E2) were analyzed using ELISA kits (Shanghai Yuangju Biotechnology Center, Shanghai, China).The antioxidant capacity of the liver was determined using SOD and MDA assay kits (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer's instructions.The liver TG and TC were assayed using assay kits (Jiancheng Bioengineering Institute, Nanjing, China).For sample preparation, a small amount (approximately 1 g) of the tissue samples was accurately weighed, and pre-cooled saline solution was added in a ratio of weight (g) to volume (mL) = 1:9, homogenized in an ice-water bath and centrifuged at 3600× g rpm for 10 min at 4 • C. The supernatant was collected and used for testing.

Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)
The total RNA was extracted from the liver samples using TRIzol reagent (Vazyme Biotech Co., Ltd., Nanjing, China).The total cDNA was synthesized with the total RNA (1 µg) using HiScript III RT SuperMix for qPCR (+gDNA Wiper) (Vazyme) and quantitative real-time PCR (RT-qPCR) amplification using the ChamQ universal SYBR qPCR Master Mix (Vazyme) and a QuantStudio ® 5 (Thermo Fisher Scientific, Inc., Waltham, MA, USA).The primer sequences used for PCR are listed in Table 2, and the relative expression of the target gene was analyzed using the 2 −∆∆Ct method after normalization against the geometric mean of expression of β-actin and GAPDH.

Histopathological Evaluation of Liver Tissues
The liver tissues were fixed in 4% paraformaldehyde for 24 h and embedded in paraffin, followed by preparation of hematoxylin-eosin (H&E staining) and Oil Red O sections.The histological samples were evaluated based on tissue structural integrity and oil amount (Service-Bio, Wuhan, China).

Statistical Analysis
All thedata were first organized using Excel software and then statistically analyzed using SPSS 20 and GraphPad Prism 7.0 software.A one-way ANOVA was used to analyze the differences among groups for multiple group data comparison, and the least significant difference (LSD) method was used for multiple comparisons with Duncan's new repolarization difference test.p < 0.05 indicated that differences were statistically significant.

Effect of PSM on Productive Performance and Egg Quality
The results of the productivity and hatching performance of the hens are shown in Table 3.Compared to the CON group, the fertilization rates and hatchability were improved in the PSM-H group (p < 0.05).The mortality rate of chickens was reduced in the PSM-H group (p < 0.05).The test of egg quality is shown in Table 4. Compared to the CON group, the Haugh unit and protein height were increased in the PSM-M group (p < 0.05), while the PSM-H group only increased the protein height (p < 0.05).There was no significant difference between each group in the average egg weight, egg shape index, yolk color, yolk weight, shell thickness or eggshell strength.The different superscript small letters were judged as a significant difference, p < 0.05, n = 6. 1 Fertilization rate (%) = (the number of incubated eggs − the number of infertile eggs)/the number of incubated eggs × 100%. 2  Hatchability (%) = the number of chicks hatched/number of eggs sent to hatch × 100%.The different superscript small letters were judged as a significant difference, p < 0.05, n = 12.

Effects of the Organ Index of Breeder Hens
The results related to the effects of dietary PSM on the organ weight, abdominal fat weight and oviduct length of breeder hens are presented in Table 6.Compared to the CON group, the PSM-M group was able to reduce their abdominal fat percentage (p < 0.05).The number of preovulatory follicles was increased in the PSM-H group (p < 0.05).The different superscript small letters were judged as a significant differences, p < 0.05, n = 6.

Effects of PSM on Plasma and Liver Biochemical Variables
The results related to the effects of dietary PSM on the serum and liver biochemical variables are presented in Table 7.Among the indicators related to lipid metabolism, TG in both the serum and liver of the PSM-M group were significantly reduced (p < 0.05).The same results were observed for the serum LDL-C in the PSM-M group (p < 0.05).The serum GLU value decreased in all the PSM groups (p < 0.05).Based on the results of the antioxidant capacity test, serum T-AOC was increased in the PSM-M and PSM-H groups (p < 0.05), and SOD in both the serum and liver was significantly increased in all the PSM groups (p < 0.05).The serum ALT was significantly decreased in the PSM-M group (p < 0.05).The sex hormone serum estradiol (E2) was significantly increased in the PSM-H group.The different superscript small letters were judged as a significant differences, p < 0.05, n = 6.

Effects of Dietary PSM on the Liver Tissue of Breeder Hens
The effect of PSM on the liver tissue is shown in Figure 2. According to the H&E figure, we found the presence of microvesicular steatosis to medium vesicular steatosis in the CON group.This suggested that hepatocellular steatosis (HS) occurred in the CON group.However, the extent of this HS decreased with increasing PSM doses.Oil Red O staining shown orange-red lipid droplets that were visible in the stem cells of the control group but were reduced in the treated group.

Effects of Dietary PSM on the Liver Gene Expression Levels of Breeder Hens
Figure 3 summarizes the effects of dietary PSM on the expression of mRNA genes related to antioxidant and lipid metabolism in the liver.The PSM-H diet significantly upregulated the mRNA expression of Nrf2, GSH-Px, CAT, NQO-1 and HO-1 (p < 0.05).Compared with the CON group, all the PSM groups reduced their mRNA expression of FAS (p < 0.05).The mRNA expression of LXRα was reduced in both the PSM-M and PSM-H groups (p < 0.05).Furthermore, the mRNA expression of ACC and SREBP1c was decreased in the PSM-M group (p < 0.05).

Effects of Dietary PSM on the Liver Gene Expression Levels of Breeder Hens
Figure 3 summarizes the effects of dietary PSM on the expression of mRNA genes related to antioxidant and lipid metabolism in the liver.The PSM-H diet significantly upregulated the mRNA expression of Nrf2, GSH-Px, CAT, NQO-1 and HO-1 (p < 0.05).Compared with the CON group, all the PSM groups reduced their mRNA expression of FAS (p < 0.05).The mRNA expression of LXRα was reduced in both the PSM-M and PSM-H groups (p < 0.05).Furthermore, the mRNA expression of ACC and SREBP1c was decreased in the PSM-M group (p < 0.05).

Effects of Dietary PSM on the Liver Gene Expression Levels of Breeder Hens
Figure 3 summarizes the effects of dietary PSM on the expression of mRNA genes related to antioxidant and lipid metabolism in the liver.The PSM-H diet significantly upregulated the mRNA expression of Nrf2, GSH-Px, CAT, NQO-1 and HO-1 (p < 0.05).Compared with the CON group, all the PSM groups reduced their mRNA expression of FAS (p < 0.05).The mRNA expression of LXRα was reduced in both the PSM-M and PSM-H groups (p < 0.05).Furthermore, the mRNA expression of ACC and SREBP1c was decreased in the PSM-M group (p < 0.05).Values are the mean ± SD, n = 6.The different superscript small letters were judged as a significant differences, p < 0.05.

Discussion
It was found that the addition of 300 mg/kg or 250 mg/kg of PSE to the diet significantly increased the rate of egg production, egg qualification, fertilization and hatching, while reducing the mortality of chickens [30,31].In contrast, Sun [32] found that replacing soybean meal with 10 to 20% PSM in the ration had no significant impact on egg production rate.In this experiment, we used PSM directly instead of PSM extracts.The addition of PSM to the diet had no significant effect on egg production rates.However, the fertilization rate and hatchability rate improved when the diet was supplemented with 1% PSM.In addition, the 0.6% PSM diet significantly reduced the mortality of the hens.Further research is needed to determine whether directly increasing the PSM dose can improve egg production, or whether an active ingredient in PSE increases the production of laying hens.The results showed that adding 0.6% PSM to the diet significantly increased the eggs' protein contents and Haugh units.These results are inconsistent with those of Sun [32].They observed no significant effect of the 10 to 20% percent PSM diet combination on the protein contents or Haugh units of the eggs.These differences may be related to the different types of PS.
Previous studies have shown that PS supplementation in the diet of laying hens significantly increases the levels of PUFAs in their eggs [26,27].Consistent with previous research, dietary PSM had a significant impact on the fatty acid composition of the egg yolks.MUFAs and PUFAs were increased in all the PSM groups.PSM still contains a certain amount of oil after extraction.These oils should enter the egg via the hen and thereby influence the fatty acid composition of the yolk [33].Values are the mean ± SD, n = 6.The different superscript small letters were judged as a significant differences, p < 0.05.

Discussion
It was found that the addition of 300 mg/kg or 250 mg/kg of PSE to the diet significantly increased the rate of egg production, egg qualification, fertilization and hatching, while reducing the mortality of chickens [30,31].In contrast, Sun [32] found that replacing soybean meal with 10 to 20% PSM in the ration had no significant impact on egg production rate.In this experiment, we used PSM directly instead of PSM extracts.The addition of PSM to the diet had no significant effect on egg production rates.However, the fertilization rate and hatchability rate improved when the diet was supplemented with 1% PSM.In addition, the 0.6% PSM diet significantly reduced the mortality of the hens.Further research is needed to determine whether directly increasing the PSM dose can improve egg production, or whether an active ingredient in PSE increases the production of laying hens.The results showed that adding 0.6% PSM to the diet significantly increased the eggs' protein contents and Haugh units.These results are inconsistent with those of Sun [32].They observed no significant effect of the 10 to 20% percent PSM diet combination on the protein contents or Haugh units of the eggs.These differences may be related to the different types of PS.
Previous studies have shown that PS supplementation in the diet of laying hens significantly increases the levels of PUFAs in their eggs [26,27].Consistent with previous research, dietary PSM had a significant impact on the fatty acid composition of the egg yolks.MUFAs and PUFAs were increased in all the PSM groups.PSM still contains a certain amount of oil after extraction.These oils should enter the egg via the hen and thereby influence the fatty acid composition of the yolk [33].
In rats fed a low-fat diet (7% by weight), the weight of their epididymal adipose tissue was slightly but not significantly lower than a perilla pomace-fed group [34].The abdominal fat index of laying hens was reduced in the 0.6% PSM group in this experiment.According to Anene [35], poor feed efficiency in chickens leads to increased abdominal fat pad weight and liver weight, eventually leading to fatty liver hemorrhagic syndrome.PSM containing rosmarinic acid can improve nutrient digestibility [36].PSM could reduce the abdominal fat index by improving feed digestibility in hens.
The hormone FSH can promote the growth of immature follicles in the ovary and increase the rate of egg laying [37].Previous studies have shown that E2 levels are closely related to laying performance, with E2 concentrations being highest during peak laying periods [38].In this experiment, E2 was significantly increased in the 1% PSM group.The flavonoids found in PSE have weak estrogenic activity and can bind to estrogen receptors in vivo, resulting in bidirectional regulation of animal performance [39].It is likely that for this reason, the number of preovulatory follicles and serum E2 levels of the hens could be increased by the flavonoids contained in the 1% PSM diet.
The SOD and GSH-Px are important antioxidant enzymes.The activity of T-AOC demonstrates its ability to eliminate oxygen radicals in the body.Another indicator was MDA, one of the end products of lipid peroxidation and a marker of oxidative stress [40].Oxidative stress is caused by the excessive accumulation of reactive oxygen species (ROS).Excessive ROS accumulation leads to damage to macromolecules such as proteins, lipids and nucleic acids in the body, ultimately causing disease [41].Rosmarinic acid plays an antioxidant role by reducing the production of reactive oxygen species, regulating lipid peroxides and improving the activity of antioxidant enzymes [42].The polysaccharides in PSM exhibit antioxidant potential by scavenging free radicals [43].This reduces H 2 O 2induced intracellular ROS generation in cells by increasing GSH, SOD and CAT levels and decreasing MDA levels [43].Rosmarinic acid in PSM activates the antioxidant defense mechanism system via the Nrf2-mediated pathway and alleviates heat stress in laying hens [36,44].Activation of the Nrf2/HO-1 signaling pathway can increase the expression of various antioxidant genes and reduce oxidative stress in tissues; for example, by increasing the expression of CAT, SOD, HO-1 and GSH synthesis [45,46].Similar to the above results, the PSM diet upregulated the gene expression of factors related to liver antioxidant and lipid metabolism, as well as serum SOD and T-AOC levels.These studies suggest that PSM may increase antioxidant capacity through multiple pathways.However, no significant reduction in MDA was observed, and further animal and cell studies are needed to explore this reason.
Food sources rich in PUFAs typically contain antioxidants and may also regulate glucose or cholesterol metabolism [47].The PUFAs in PSM are thought to affect the lipid metabolism of chickens, which in turn alters the corresponding glucose and lipid indicators.Glucose content was an important indicator of insulin resistance and hyperglycemia, which was reduced by the PSM diet in the chickens in these experiments [48].According to Cha et al., perilla oil (0.1 or 0.3%) not only reduced the development of hypercholesterolemia and atherosclerosis in rabbits on a high-cholesterol diet (HCD), but also reduced fat accumulation and lipid peroxidation in their liver and kidney tissues [49].After absorption from the intestinal tract, TG is collected in the liver and enters the bloodstream as a component of LDL-C.To evaluate the health status of birds, blood lipid levels such as glucose, TG, TCH, HDL-C and LDL-C are usually analyzed.Dietary PSE decreased serum levels of TC, TG and LDL-C but increased HDL-C in mice on a high-fat diet [23,50].We found that PSM reduced serum TC, TG and LDL-C in chickens.It also reduced TC and TG in the liver.These results are consistent with those of previous studies.Furthermore, TG is the main component of lipid droplets in hepatocytes [51].These findings are consistent with the results of H&E staining and Oil Red O staining of liver tissue samples.
LXRα is highly expressed in liver tissue as a member of the nuclear receptor superfamily.It activates its important downstream target gene, SREBP1, which in turn regulates genes related to lipid synthesis [52], thus contributing to the regulation of lipid metabolism [52,53].The main function of SREBP-1c is to regulate fatty acid and triglyceride synthesis.Overexpression of this protein leads to the accumulation of lipids in non-adipose tissues, ultimately leading to tissue damage.The genes of ACC and FAS are the target genes associated with SREBP-1c.They are related to lipid synthesis and glucose metabolism [54,55].They also function as two rate-limiting enzymes involved in fatty acid synthesis, and their activities may reflect the body's ability to synthesize fat to some extent [56].The CYP7A1 gene plays a crucial role in regulating cholesterol homeostasis and bile acid biosynthesis.Similarly, Zhang et al. found that perilla pomace significantly reduced hepatic FAS activity in rats [34].Based on the expression of ACC and FAS genes, it is speculated that PSM leads to a decrease in lipid synthesis levels by downregulating ACC and FAS expression, leading to a decrease in serum TG.Meanwhile, PSM inhibits lipid synthesis and lowers glucose levels via the LXRα/SREBP1 signaling pathway.

Conclusions
In conclusion, the various nutrients contained in PSM can improve the antioxidant capacity of laying hens and regulate lipid metabolism in their livers.PSM improved blood lipids and reduced mortality in chickens by protecting their livers.At the same time, the high-quality oils in PSM can reach the eggs via the hens and help improve egg quality.We recommend the adding 0.6% PSM to the leyer fed, which improves the physical condition of the hens and brings greater economic benefit.

Table 1 .
The ingredient and nutrient composition of the basal diet (% as fed basis).

Table 2 .
Laying performance of breeder hens.

Table 3 .
Effect of dietary PSM on productive and hatching performance of breeder hens.

Table 4 .
Effects of dietary PSM on egg quality.

Table 5 .
Effect of PSM on egg yolk fatty acid contents.

Table 6 .
Effects of dietary PSM on the organ index of breeder hens.

Table 7 .
Effects of dietary PSM on biochemical variables in the serum and liver.