Licury Cake in Diets for Lactating Goats: Qualitative Aspects of Milk and Cheese

Simple Summary Feed costs are high in feedlot systems. To reduce these costs, cheaper alternatives are studied. The use of licury cake (LC) is an economical alternative because it can compete (economically and nutritionally) with common ingredients used in animal feed. However, the ruminant has different feed degradability and digestibility mechanisms, promoted by symbiosis with microorganisms in the rumen that can affect the physicochemical characteristics of the final product. We studied dietary LC inclusion, at levels of 0, 67, 133 and 200 g/kg of dry matter (DM), to evaluate its impact on qualitative aspects of milk and cheese. The inclusion of LC up to 133 g/kg DM is recommended for the diet of lactating goats whose milk will be used in the production of Minas Frescal cheese. Abstract The study was carried out to evaluate the effects of licury cake (LC) inclusion in diets for lactating goats on milk chemical composition and fatty acid profile, and physicochemical composition and sensory attributes of Minas Frescal cheese. Twelve lactating goats were used (eight Saanen and four Anglo Nubian goats, with 35 ± 5 days in lactation and average body weight of 37.93 ± 9.22 kg), distributed in a triplicate 4 × 4 Latin Square design. The increasing levels of LC studied were: 0, 67, 133 and 200 g/kg of dry matter (DM). The analyses were adjusted using orthogonal polynomial contrasts, in which the probability level of 5% was considered. For sensory attributes, principal component analysis was performed. The LC inclusion promoted the reduction in moisture (p = 0.013) and mineral matter (p < 0.001) content in cheese. The ether extract content in cheese showed a quadratic effect (p = 0.021). Lauric acid showed a linear (p = 0.002) increasing effect, and myristic (p < 0.001) and rumenic (p = 0.018) acids showed quadratic effects. The sensory attributes analysis in cheese indicated that the inclusion of 133 and 200 g/kg of LC improve its texture. Flavor was improved with the inclusion of 67 and 133 g/kg. The inclusion of up to 133 g/kg of LC in the diet of lactating goats is recommended because it promotes improvements in flavor and texture of Minas Frescal cheese.


Introduction
The largest goat herd in Brazil is found in tropical regions where goat breeding is predominantly in extensive production systems. In these systems, the feed base is native forage, which is affected in terms of availability and quality (high lignin and low protein levels) depending on the time of year, negatively affecting the productive performance of the animals [1].
Breeding in intensive or semi-intensive systems is a strategy used to avoid low feed availability. The quality of the feed is superior in these systems, avoiding variations in milk Federal University of Bahia (UFBA). The experiment was conducted in the goat breeding section of the "Entre Rios" Experimental Farm-UFBA, located in the municipality of Entre Rios, Brazil (11 • 56 31 S, 38 • 05 04 W, 162 m above sea level).

Animals, Experimental Design and Management
Twelve multiparous lactating goats (eight Saanen and four Anglo Nubian goats; average weight of 46.9 ± 9.4, averaging 105 ± 5 days in milk; and with an average production of 1.5 ± 0.4 kg/day) were used. Animals came from the goat area of the "Entre Ríos" Experimental Farm-UFBA, and were selected considering the uniformity in breed anatomical characteristics, body weight, days in milk, and milk production. Goats were distributed in a triplicate 4 × 4 Latin square design ( Figure 1).

Ethics Committee and Experiment Location
The experiment followed animal welfare rules, and thus the project was approved (approval no. 73/2018) by the Ethics Committee of the Use of Animals (CEUA) at the Federal University of Bahia (UFBA). The experiment was conducted in the goat breeding section of the "Entre Rios" Experimental Farm-UFBA, located in the municipality of Entre Rios, Brazil (11°56′31″ S, 38°05′04″ W, 162 m above sea level).

Animals, Experimental Design and Management
Twelve multiparous lactating goats (eight Saanen and four Anglo Nubian goats; average weight of 46.9 ± 9.4, averaging 105 ± 5 days in milk; and with an average production of 1.5 ± 0.4 kg/day) were used. Animals came from the goat area of the "Entre Ríos" Experimental Farm-UFBA, and were selected considering the uniformity in breed anatomical characteristics, body weight, days in milk, and milk production. Goats were distributed in a triplicate 4 × 4 Latin square design ( Figure 1). Before the beginning of the experiment, fifteen days were used to adapt the animals to the facilities, milking management and concentrate content of diets. The study was divided into four periods of 14 days each. Therefore, the total duration of the study was 56 days. Each period was divided into ten days for the adequacy of the animals to the byproduct in the diets and four days for the data and samples collection.
The goats were housed in 1.5 m 2 individual pens; equipped with drinker and feeding trough. Water was provided ad libitum. Feed was supplied with daily adjustments to allow around 10% refusals.
Diets were formulated according to the NRC [15] to meet the requirements for maintenance and milk production of lactating goats. The experimental diets consisted of increasing inclusion rates of LC: 0, 67, 133 and 200 g/kg DM (Table 1) and fed as a total mixed ration, twice daily (08:00 h and 15:00 h). A forage:concentrate ratio of 40:60 was adopted, with maize silage used as the forage. Table 1. Proportion of ingredients and analyzed chemical composition of the diets including licury cake.

Variable
Licury Before the beginning of the experiment, fifteen days were used to adapt the animals to the facilities, milking management and concentrate content of diets. The study was divided into four periods of 14 days each. Therefore, the total duration of the study was 56 days. Each period was divided into ten days for the adequacy of the animals to the by-product in the diets and four days for the data and samples collection.
The goats were housed in 1.5 m 2 individual pens; equipped with drinker and feeding trough. Water was provided ad libitum. Feed was supplied with daily adjustments to allow around 10% refusals.
Diets were formulated according to the NRC [15] to meet the requirements for maintenance and milk production of lactating goats. The experimental diets consisted of increasing inclusion rates of LC: 0, 67, 133 and 200 g/kg DM (Table 1) and fed as a total mixed ration, twice daily (08:00 h and 15:00 h). A forage:concentrate ratio of 40:60 was adopted, with maize silage used as the forage.
Hand milking (once a day) was performed at 07:00 h, after pre-dipping with a 0.5% glycerin iodine solution. After milking, post-dipping was performed using a 0.5% glycerin iodine solution. Hygiene procedures were followed to avoid mammary gland infections. The average body weight of the goats was obtained after the pre-adaptation period and on the first and last day of each period (day 1 and 14). The animals were weighed before supplying the diets, in the morning and with the help of an electronic scale.

Chemical Analysis
Samples of experimental diets (ingredients, concentrate, and forage) and refusals were collected the last seven days of each period. Refusals (all food that was not eaten by animals) were collected before the first feeding; the sampling of the ingredients was carried out before the elaboration of the diets, ensuring the collection of a representative sample, and the concentrate and forage were collected once a day in the morning. Each sample was pre-dried in a forced ventilation oven (55 • C for 72 h). Afterwards, samples were ground in a Wiley-type blade mill to 1 mm for the determination of the chemical composition and to 2 mm for the determination of non-digestible neutral detergent fiber. Dry matter (934.01), ashes (930.05), crude protein (CP, 981.10), and ether extract (EE, 920.39) were determined following the methodology proposed by the Official Association of Chemical Analysis [16].
Neutral detergent fiber (NDF) and acid detergent fiber (ADF) determinations were performed according to Van Soest et al. [17]. FDN and FDA were corrected for ash and protein (NDFap and ADFap) following the methodology described by Mertens [18] and Licitra et al. [19], respectively. Lignin was determined according to the AOAC 973.18 method [20].
The estimates of non-fibrous carbohydrates (NFC) were made according to the formula proposed by Hall [21] and Detmann and Valadares Filho [22], considering, or not, the use of urea in the diet formulation.
The non-digestible neutral detergent fiber (iNDF) was determined by incubating the samples (In Situ method, using 100 g m 2 TNT bags) according to the methodology described by Reis et al. [23]. The potentially digestible neutral detergent fiber (pdNDF) was obtained by the difference between NDFap and iNDF.

Chemical Composition of Milk
Milk production per animal and per day was recorded and sampled during the last four days of each period. One representative sample, approximately 40 mL per animal (Totaling 48 samples per period), was stored in a plastic bottle containing the preservative 2-bromo-2-nitropropane-1,3-diol (bromopol). These samples were analyzed in the Bentley Instruments ® Infrared Analyzer (Bentley Instruments Inc., Curitiba, Paraná, Brazil) to determine the contents of protein, fat, lactose, urea nitrogen and total solids. Somatic cell counting was performed using the Bentley Instruments ® Soma-count-500 device (Bentley Instruments Inc., Curitiba, Paraná, Brazil). These analyses were performed in the laboratory of the Clinica do Leite ESALQ/USP, Piracicaba-SP. To obtain the content of milk components (g/day), the percentage of each component (fat, protein, lactose, and total solids) was multiplied by the volume of milk produced (g/day). The milk and cheese were weighed using a computerized balance Toledo Prix 4 Uno with a printer.

Fatty Acid Profile of Milk
Milk samples intended for fatty acid analysis were stored in airtight containers and frozen at −20 • C. The fat of these samples was extracted with the organic solvent hexane. Fatty acid methylation was carried out in two steps, using basic (sodium methoxide) and acid (acetyl chloride) catalysts [24].
The quantification of fatty acid methyl esters was performed by gas chromatography (Focus GC-Thermo Scientific; Thermo Fisher Scientific; São Paulo, Brazil), with a flame ionization detector (CG-DIG) and SP-2560 capillary column (Supelco, 100 m × 0.25 mm × 0.2 µm). The gas flow was 1.5 mL/min of H 2 (carrier gas). The detector and injector temperatures were set at 250 • C. The temperature gradient used was had an initial temperature of the column established at 70 • C, maintained for 4 min, and then the temperature was raised to 175 • C at a rate of 13 • C per minute, maintained at this temperature for 27 min, increasing again at 215 • C, at a rate of 4 • C per minute, maintaining this temperature for 31 min [24]. The identification of fatty acid methyl esters was based on retention times of FA 275 standards (GLC-674, Nu-Chek Prep, Inc.).

Cheese Production and Yield
Milk destined for cheese production was collected in the last four days of each period. This was weighed, sieved, and stored in hermetic containers at −20 • C until the cheese was made.
For the preparation of the cheese, the milk was slowly thawed in the refrigerator. The elaboration of the Minas Frescal cheese was carried out following the sanitary norms recommended in Ordinance N • 326 of the Ministério da Saúde, which guarantees safety and quality in food manufacturing [28].
Initially, the milk was pasteurized at 60 • C for 30 min. For this process, an electric gas stove, 4 pots and a thermometer were necessary to pasteurize the milk collected by animal/treatment/period. Immediately, it was cooled in an ice bath, 38 • C. Minas Frescal cheese was produced according to the method described in Malheiros et al. [29], using as ingredients potassium chloride (0.02%; Rica Nata, Minas Gerais, Brazil), cultures provided by skimmed natural yogurt (1.8%; Nestlé), sodium chloride (0.8%; Sal Lebre), and liquid coagulant (CHY-MAX ® ). The curds were placed in cylindrical forms (perforated and sterilized) and kept at room temperature, turning every 1 h until the final dripping. Four cheeses were obtained per treatment.
Cheeses were packed and kept refrigerated at 4 ± 1 • C for approximately 24 h, until the physicochemical and sensory analyses. Afterwards, cheese samples were collected for physicochemical analysis, including humidity (gravimetric method).
The milk and cheese were weighed using a computerized balance Toledo Prix 4 Uno, Toledo do Brazil, São Paulo, Brazil with a printer.

Sensorial Analysis
Sensory analysis was performed by applying a questionnaire [31], in which five sensory attributes were evaluated: color, odor, flavor, texture, and global acceptance. The sensory analysis was evaluated according to the following nine-point scale: dislike extremely (1), dislike very much (2), dislike moderately (3), dislike slightly (4), neither like nor dislike (5), like slightly (6), like moderately (7), like very much (8), and like extremely (9). The test was carried out with a panel of 103 untrained tasters, previously selected as consumers of dairy products, who did not suffer from allergies and who were interested in participating in the sensory analysis. Of the 103 tasters, 67.3% were women and 36.7% men, 85.1% were between 18-30 years old, 8.6% between 31-40 years old, 4.9% between 41-50 years old, and 1.0% between 51-60 years old. The frequency of consumption of goat's cheese by the tasters was: 90.6% consume it rarely, 8.9% sporadically, and 1% frequently.
Each taster received four samples of fresh cheese (1.5 × 1.5 × 1.5) corresponding to the four inclusion levels of LC (0, 67, 133 and 200 g/kg DM), salt and water biscuit, and mineral water. It was recommended that between samples. The biscuit and water have the function of cleaning the taste buds; therefore, the previous sample would not interfere with the sensory analysis of the later sample [32]. Samples were coded with three random digits and supplied in sealed pots to maintain sensory characteristics. The evaluation was carried out in the morning, between 09:00 and 12:00 h.

Statistical Analysis
For the analyses, SAS (Statistical Analysis System) statistical software version 9.2 [33] was used. The variables of intake, digestibility, feeding behavior, milk production, and nitrogen metabolism were assessed according to a triplicated 4 × 4 Latin Square. The mathematical model below was applied: whereŶ ijkl = dependent variable; µ = overall mean; LS i = fixed effect of the Latin Square (i = 1, 2 and 3); A(LS i ) j = random effect of the animal into the Latin Square (j = 1, 2, 3 and 4); P k = random effect of the period (k = 1, 2, 3 and 4); LC l = effect of the LC inclusion level (l = 0, 67, 133 and 200 g kg −1 ); LS i × LC l = fixed effect of the interaction between Latin Square and LC inclusion level; and ε ijkl = random experimental error associated with each observation, with NID~(0, σ2) assumption.
Furthermore, the effect of the LC inclusion level was evaluated using Orthogonal Polynomial Contrasts to determine the linear (−3, −1, +1, +3) and quadratic (+1, −1, −1, +1) effects. For all the evaluations, the level of 5% probability of type I error (p ≤ 0.05) was considered. No interaction between treatment and racial group was observed for any of the variables studied.
The sensory analysis constituted a set of multivariate data that were arranged in a matrix (412 × 6) and interpreted using principal component analysis (PCA). For this analysis, SAS software version 9.4 (SAS Institute Inc.; Cary, NC, USA) was used with data centered on the mean.
No breed effect or interaction between treatment and breed group was observed for any of the variables studied.

Nutritional Components Intake and Milk Chemical Composition
There were no differences in the nutritional components' intake or in milk composition (p > 0.05) promoted by increasing levels of LT inclusion in the diet of lactating goats ( Table 2). The average DM intake was 1.61 kg/day and of protein was 0.3 kg/day. The mean values observed for fat, protein, lactose, and total solids were 39.4; 33.9; 45.5, and 126.5 g per day, respectively.

Quality of Minas Frescal Cheese
The inclusion of LC gradually reduced the ash content (p < 0.001) of the cheese. On the other hand, moisture and ether extract showed quadratic behavior with a minimum value of 53.89% with the inclusion of 116.7 g of LC/kg DM (p = 0.001) and a maximum value of 47.04% with the inclusion of 154.72 g of LC/kg DM (p = 0.021), respectively. Crude protein concentration and yield were not affected (p > 0.05) by the inclusion of LC in the diet of lactating goats (Table 5). Table 5.
Chemical composition and yield of cheese made from the milk of lactating goats fed diets with increasing levels of licury cake.

Sensorial Analysis
Principal components analysis allows us to observe the distribution of treatments on specific characteristics that influence consumer preferences. The first two main components (PC1 and PC2) explained 99.13% of the variance of the sensory parameters evaluated. The principal component 1 is represented by the cheese texture and the principal component 2 by the cheese flavor (Table 6). The distribution of the treatments within the first principal component indicates that the treatments with LC inclusion of 133 and 200 g/kg DM had a more pleasant texture compared to the treatments with less inclusion. As for main component 2, cheese flavor was preferred when LC inclusion was 67 and 133 g/kg DM (Figure 2).

Nutritional Components Intake and Milk Chemical Composition
The dry matter intake directly affects the intake of the other nutritional components of the diet, which may explain the similarity in CP intake. Although LC is a fibrous feed (64% NDF), the physical form in which it was added to the diet (ground) did not affect feed degradability or ruminal passage rate, so it did not affect DM intake. Similar behavior on DM and CP intakes was observed by Bagaldo et al. [7], when evaluating increasing levels of LC in diets for feedlot lambs. It is important to note that the palatability of LC was not a limiting factor for dietary intake in lactating goats.
Contrary to our results, in Silva et al. [11] and Silva et al. [6], dry matter and crude protein intake decreased in cull cows and goat kids, respectively. However, those results probably correlate with the high-concentrate diet (20:80-forage:concentrate) these animals received compared to the 50:50 diet used in the current experiment. Therefore, it is possible to infer that there is a limitation in the use of LC in high-concentrate diet for ruminants.
The chemical composition of milk is directly proportional to nutrient intake [34]. Therefore, the similarity in DM intake resulted in similar nutrient availability in the ruminal and post-ruminal environments. Consequently, the chemical composition of milk was similar between treatments, regardless of the LC inclusion in the diet. Ferreira et al. [3] observed that the inclusion of LC of up to 144 g/kg DM in the supplement for grazing lactating cows did not affect the milk chemical composition. On the other hand, it is possible to hypothesize that the proximal composition of the product is not usually affected by the nutritional conditions of diets without an excess of a specific nutrient, as observed in Silva et al. [13] and Silva et al. [6]. In these studies, the proximal composition of meat has not been altered by the inclusion of LC in the diet of beef cattle and goat kids, respectively.

Fatty Acid Pofile of Milk
Short-and medium-chain fatty acids are synthesized in the mammary gland from acetate and β-hydroxybutyrate compounds produced in the rumen [35]. As the similarity in nutrient intake is reflected in similar ruminal fermentation, it was also expected that fatty acid content would be similar in the current study.

Nutritional Components Intake and Milk Chemical Composition
The dry matter intake directly affects the intake of the other nutritional components of the diet, which may explain the similarity in CP intake. Although LC is a fibrous feed (64% NDF), the physical form in which it was added to the diet (ground) did not affect feed degradability or ruminal passage rate, so it did not affect DM intake. Similar behavior on DM and CP intakes was observed by Bagaldo et al. [7], when evaluating increasing levels of LC in diets for feedlot lambs. It is important to note that the palatability of LC was not a limiting factor for dietary intake in lactating goats.
Contrary to our results, in Silva et al. [11] and Silva et al. [6], dry matter and crude protein intake decreased in cull cows and goat kids, respectively. However, those results probably correlate with the high-concentrate diet (20:80-forage:concentrate) these animals received compared to the 50:50 diet used in the current experiment. Therefore, it is possible to infer that there is a limitation in the use of LC in high-concentrate diet for ruminants.
The chemical composition of milk is directly proportional to nutrient intake [34]. Therefore, the similarity in DM intake resulted in similar nutrient availability in the ruminal and post-ruminal environments. Consequently, the chemical composition of milk was similar between treatments, regardless of the LC inclusion in the diet. Ferreira et al. [3] observed that the inclusion of LC of up to 144 g/kg DM in the supplement for grazing lactating cows did not affect the milk chemical composition. On the other hand, it is possible to hypothesize that the proximal composition of the product is not usually affected by the nutritional conditions of diets without an excess of a specific nutrient, as observed in Silva et al. [13] and Silva et al. [6]. In these studies, the proximal composition of meat has not been altered by the inclusion of LC in the diet of beef cattle and goat kids, respectively.

Fatty Acid Pofile of Milk
Short-and medium-chain fatty acids are synthesized in the mammary gland from acetate and β-hydroxybutyrate compounds produced in the rumen [35]. As the similarity in nutrient intake is reflected in similar ruminal fermentation, it was also expected that fatty acid content would be similar in the current study.
The increase in lauric and myristic acids in meat (Longissimus lumborum muscle) was also observed by Costa et al. [14]. The authors evaluated the inclusion of up to 240 g/kg DM in the diet of feedlot lambs. The linear increase in lauric acid in milk was also reported by Porto Junior et al. [37] when LC was included up to 165 g/kg DM in the diet.
The importance of lauric acid in milk is positive, considering that the intake of medium chain fatty acids (MCFA) has been reported to promote benefits for human health. CMFA are more rapidly digested and absorbed due to their small size, which is a favorable feature, as these are also used as an energy source [38].
The increase in the concentration of rumenic acid (18:2 cis9trans11) in milk with the inclusion of 10.1 g of LC/kg of DM in the diet is related to the greater activity of the enzyme delta-9-desaturase, since it is known that 64% of the conjugated linoleic acid (CLA) present in milk comes from the desaturation of vaccenic acid (18:1 trans11) in the mammary gland [39].
Among the isomers of conjugated linoleic acid, rumenic acid (18:2 cis9trans11) predominates in the milk of ruminant animals. This fatty acid is correlated with the prevention of the appearance of cancer and coronary diseases [40]. Therefore, the increase in rumenic acid with the inclusion of 10.1 g of LC/kg DM in the diet indicates an improvement in the quality of milk fat.
The fatty acids that were significant in the goat milk of the current experiment were also significant in the meat fatty acid profile of goat kids in the study by Silva et al. [6]. However, the fatty acid profile was not affected in meat from beef cattle [13]. Therefore, it is important to note that rumen lipid metabolism is more marked in small ruminants. In this sense, it is important to take into account the level of LC inclusion that will improve the fatty acid profile considering human health.

Quality of Minas Frescal Cheese
The quadratic effect observed for cheese moisture was concave, while the curve for cheese EE content was convex, indicating an inverse relationship between these variables. The opposite behavior of these two variables can be attributed to the higher enzymatic activity of lipoprotein lipases and esterases (enzymes used for cheese production) in the moister cheeses, which probably favored lipolysis, which was reflected in the EE content [41,42].
Some factors can interfere with the yield of the cheese, among which we can mention the chemical composition of the milk, the methodology used, the activity of the enzymes, among others [43].
Consequently, the similarity in cheese yield was due to the similarity in the chemical composition of the milk between the diets studied as well as the control of the production process.

Sensorial Analysis
The use of multivariate methods such as principal component analysis (PCA) had the purpose of being able to observe the results from another perspective. In the current study, this analysis indicated that the first two principal components explained 99.13% of the total variation of the sensory analysis of Minas Frescal cheese. The attributes with the greatest influence on the sensory evaluation of the cheese were texture (Principal Component 1) and flavor (Principal Component 2).
It was observed that the inclusion of LC at the levels of 133 and 200 g/kg DM improved the texture of the cheese. On the other hand, the intermediate levels of LC inclusion, 67 and 133 g/kg DM resulted in a more pleasant cheese flavor, and, consequently, a higher global acceptance.
Childs and Drake [44] reported that reduction in cheese fat content is associated with changes in texture, with reductions in cheese chewiness, hardness, and elasticity, reflecting product acceptability to consumers. Therefore, the observed increase in the fat content of the cheeses due to the LC inclusion was reflected in a higher score for the cheese texture parameter.
It is known that changes in the cheese chemical composition are reflected in the flavor. These differences are mainly related to the occurrence of reactions such as proteolysis, lipolysis and carbohydrate fermentation. However, the presence of phenolic compounds is also associated with sensory changes. Phenolic compounds in adequate concentrations in milk and its derivatives have a positive relationship with the sensory quality of the product; however, in excessive concentrations it negatively affects cheese flavor and odor [45]. The licury cake has phenolic compounds, such as epicatechia, catechin, procyanidin B1 and B2, and luteolin, among others, which can influence the sensory acceptance of cheese when goats are fed with this by-product. However, the most probable hypothesis is that the intermediate levels of inclusion of LC (67 and 133 g/kg) increased acceptance of cheese flavor due to the presence of these phenolic compounds and how they are sensed by the taste buds. On the other hand, the higher inclusion level of LC (200 g/kg DM) promoted a higher concentration of phenolic compounds, which negatively affected the cheese flavor.
On the other hand, it is observed that unsaturated fatty acids, myristoleic and rumenic acids, increased linearly in the milk that was used to make cheese following LC inclusion. The increase in these fatty acids is likely to improve the flavor characteristics of the cheese; however, in excess, these fatty acids promote a certain degree of oxidation that promotes a rancid taste unpleasant for the consumer.

Lipids and Human Health
In recent decades, humans have been very concerned about nutrition, mainly the lipid content of the diet. This concern is due to the content of saturated and trans FA, which are highly correlated with the development of cardiovascular, metabolic, physiological, and even psychological diseases, directly or indirectly [46]. According to Sacks et al. [47], replacing the SFA content with PUFA can decrease the incidence of cardiovascular disease (CVD) by 30%.
Ruminant products are sources of SFA and trans FA, and for these reasons ruminant products are being censored in human nutrition [48]. As observed in the present study, the SFA (68.9%) and MUFA (26.6%) FAs presented the highest proportion of the total FAs. It is important to point out that LC inclusion in the diets does not influence this content. However, LC inclusion increases the fat content of cheese produced from the milk of lactating goats fed diets containing increasing levels of LC. Therefore, although the fatty acid profile is similar in the unprocessed material (milk), the different content of fat in the final product (cheese) can consequently affect the fatty acid content. This consideration is important because some studies conclude that the content of SFA and trans FA and its correlation with diseases is not related to a specific content of nutrients, rather it is important to consider the general distribution of macronutrients [49].

Conclusions
As licury cake inclusion did not affect cheese yield or feed intake, based on the parameters studied, the inclusion of LC of up to 133 g/kg DM in the diet is recommended, since it preserves the maximum sensorial quality of the cheese produced from the milk of feedlot lactating goats.