Hepatoprotective Effect of the Penthorum Chinense Pursh Extract against the CCl4-Induced Acute Liver Injury via NF-κB and p38-MAPK PATHWAYS in Dogs

Simple Summary Traditional Chinese herbal medicines have been used to treat animal diseases and play an important role in the treatment of liver injury. The current study used Penthorum Chinense Pursh extract (PCPE) to treat acute liver injury of dogs caused by CCl4. Our study found that PCPE reduced the pathological symptoms and liver tissue lesions caused by ALI, improved serum biochemical indicators, and alleviated the inflammatory response and oxidative stress. Our results clarified that the NF-κB and MAPK signaling pathways in dogs are related to the antioxidant and anti-inflammatory effects of PCPE. PCPE may be used as a safe and effective medicine for the treatment of acute liver injury in dogs. Abstract Acute liver injury (ALI), manifested by acute hepatocellular damages and necrosis, is a life-threatening clinical syndrome and Penthorum Chinense Pursh (PCP) is a well-known folk medicine practiced for liver-related diseases. This study aimed to investigate the ameliorative effects of PCP extract (PCPE) on carbon tetrachloride (CCl4) induced ALI in dogs via mitogen-activated protein kinase (MAPK) and Nuclear factor κB (NF-κB) signaling pathway. Healthy dogs were induced by CCl4 and treated with different dosage regimes of PCPE for 7 days. CCl4 produced acute liver injury and induced both oxidative stress and an inflammatory response in dogs. The PCPE significantly ameliorated and improved vacuolar inflammatory lesions in liver tissues during ALI, enhanced activity of superoxide dismutase, and restored glutathione peroxidase, further significantly reducing the indices of malondialdehyde and nitric oxide in serum. Inflammatory factors (IL-1β, IL-6, and TNF-α) were declined and anti-inflammatory factors (IL-10) were increased by the application of PCPE. PCPE treatment, down-regulated the MEKK4, MKK3, p38MAPK, MSK1, and NF-κB, and upregulated the IkB mRNA levels (p < 0.01) in ALI affected dogs. In conclusion, PCPE repaired acute liver injury by improving antioxidant enzymes and by reducing oxidation products. Furthermore, the PCPE inhibited the MAPK/NF-κB signaling pathway, which resulted in anti-inflammatory and antioxidant effects on ALI-induced dogs. In the future, PCPE could be a useful ethnomedicine in veterinary clinical practices for the treatment of liver injuries or failures.


Introduction
The liver is a vital organ involved in the metabolism and detoxification of xenobiotics, chemicals, drugs, and foreign particles from the body. Acute liver injury (ALI) is a common disease of the canine family that is caused by diverse factors including drug poisoning, alcohol abuse, radioactive damage, vascular disorders, and other exogenous substances, finally leading to abnormal liver functions [1]. ALI pathogenesis is controlled by a variety of mechanisms and increases both the inflammatory response and oxidative stress, resulting in morbidity and mortality without being cured [2,3]. Carbon tetrachloride (CCl 4 ) was reported for its hepatotoxic effects, and is a widely used experimental chemical for the induction of ALI resulting in liver injury and oxidative stress [4].
Acute liver injuries or failure caused by CCl 4 -induction are associated with oxidative stress and inflammatory responses [5]. It is well established after reported studies that mitogen-activated protein kinase (MAPK) and nuclear factor kappa B (NF-κB) signaling pathways play an important role in controlling the ALI via protective mechanisms which include inflammatory response and oxidative stress [6]. The oxidative stress involved in the pathogenesis of liver injury as an intracellular serine or threonine protein kinase, MAPK can be activated by stress factors and inflammatory stimulation and plays an important role in cell proliferation, differentiation, and apoptosis [7,8]. Moreover, oxidative stress has also been reported to activate NF-κB, an important transcriptional regulator in cells, and induces NF-κB nuclear translocation to promote the expression of pro-inflammatory genes, including tumor necrosis factor α (TNF-α), Interleukin-1β (IL-1β), Interleukin-6 (IL-6) [9,10]. Therefore, the p38MAPK and NF-κB signaling pathways have been proposed as targets of the therapeutic pathway of ALI.
Recently, researchers are more focused on herbal or folk medicine for the treatment of acute liver diseases due to curative and low toxic effects [11]. Penthorum Chinense Pursh is an edible plant well-known for its usage in Chinese traditional medicine practice, reported for its therapeutic effects on infectious hepatitis, edema, and various liver diseases [12,13]. It is reported that Penthorum Chinense Pursh Extract (PCPE) has various pharmacological effects, such as anti-inflammation and anti-oxidation, and can improve liver injuries by eliminating oxygen free radicals and inhibiting inflammatory response [14,15]. These pharmacological effects are closely related to the active components of PCPE. Flavonoids, organic acids, coumarins, lignans, polyphenols, and sterols are important bioactive constituents of PCPE, and an abundance of research has demonstrated that many extracts have significant pharmacological activities of anti-inflammation and anti-oxidation [13,16].
In veterinary clinical practice, few studies have been conducted regarding the effects of PCPE and its underlying signaling mechanisms for acute liver injury, while most studies were conducted in human and cell lines [17,18]. This highlighted the necessity of further investigation of PCPE effects and related signaling mechanisms involved with acute liver injury in dogs.
Recently, studies have shown that aqueous extract of PCP and their compositions could protect against the acute and chronic CCl 4 and alcohol-induced liver injuries in mice through ameliorating oxidative stress and activating Nrf2 signaling pathways respectively [18,19]. There are very limited studies on the effect of PCPE on CCI 4 liver damages in dogs by inhibition of MAPK and NF-κB-mediated inflammatory and oxidative signaling pathways. In the present study, the CCl 4 -ALI model was established for exploring the mechanisms of PCPE medicine in reversing ALI in dogs. The protective effect might be associated with the reduced inflammatory response and oxidative stress via MAPK/NF-κB signaling pathways, however, further underlying mechanisms with respect to Autoimmune hepatitis disease are suggested for future studies. Experimental animals were managed and handled (30 • C, 56-60% humidity, ad libitum food and water) according to principles approved by the Institutional Animal Care and Use Committee, Southwest University Chongqing, China (Approval no. IACUC-20180413). Dogs were categorized into six groups (n = 8, male and female). Group-I without treatment served as control, Group-II treated with CCl 4, Group-III treated with Biphenyl Dimethyl Dicarboxylate Pills (BDDP), Group-IV, V, and VI were treated with different dosages (high-H, medium-M, low-L) of PCPE.

Materials and Methods
Firstly, dogs were divided into control and CCl 4 -induced groups. Dogs were injected 50% CCl 4 peanut oil-solution (1 ml/Kg body weight) subcutaneously in the neck for two days to establish a canine liver injury. After the establishment of CCl 4 -induced ALI, Biphenyl Dimethyl Dicarboxylate Pills (positive drug-BDDP group), and PCPE high, medium, and low dosages (1.5, 1, 0.5 herb mL −1 , respectively) were administered through gastric lavage twice a daily for 7 days till the end of the experiment. The herbal dosages of PCPE were measured based on human and animal body weight and according to Research and Evaluation of Pharmacodynamics [20]. During the experimental period daily basis clinical symptoms were recorded and monitored. The serum was withdrawn from blood samples collected from the brachiocephalic vein during the 3rd, 5th, and 7th days. And each dog was euthanized by an intravenous overdose of pentobarbital, and then liver samples were collected on the 7th day.

Preparation of PCPE
Penthorum Chinense Pursh grass was purchased from Zhongmiao Pharmaceutical Co., Ltd. (provided by Gulin, Luzhou, Sichuan Province, China). The phenotypical characteristic of grass was identified by Botanist Dr. Liu Juan from Southwest University. Grass pieces were decocted with 4000 mL of 70% ethanol solution and soaked for 4 hours, and the reflux extraction process was carried out for 90 min before filtrate was obtained. This step was repeated two times, filtrate was added 3 times after the combination of the concentrate the filter was decompressed in the rotary vacuum evaporator at 80 • C, and subsequently concentrated to 1.5 g of herb mL −1 , and stored at 4 • C.

Identification of Active Ingredients of PCPE by Infrared Spectroscopy
PCPE powder was mixed with a Potassium bromide wafer (KBr wafer), comminuted, and then pressed into a tablet for measurement, and infrared spectrum scanning was performed in the range of 4000 cm −1 to 400 cm −1 .

Criteria for Judging Efficacy
The clinical signs (mental state, exercise posture, behavior, drinking, feeding, feces/urine) of CCl 4 -induced dogs (ALI dogs) were monitored daily as the curative standard of PCPE therapy to ALI in dogs. ALI dogs or CCl 4 -induced dogs showed clinical signs including depression, weakness, loss of appetite, decreased mobility, significant wasting, choroidal congestion in the eye, yellowing of mucous membranes, abdominal bloating, oliguria with yellow urine. The clinical examination was performed after the administration of PCPE resulted in the disappearance and improvement of symptoms [21]. Finally, effective rate, cure rate, and improvement rate were calculated according to the following formula:

Histopathology of Liver
After the fixation, the liver tissues were washed, dehydrated, waxed, embedded, sectioned, and counterstained in a dehydration box using a standard method. The wellstained liver tissue sections were selected and the histopathological changes of the liverstained sections were observed under an optical microscope at 400× magnification (Zeiss upright microscope Axio Scope A1; Carl Zeiss, Oberkochen, Germany).

Biochemical Assay
The content of total bile acid (TBA) and Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), and γ-glutamyl transpeptidase (GGT) activity in canine serum were detected by automatic biochemical analysis according to the manufactures kit instructions (Beckman Coulter AU680, Beckman, America).

RT-qPCR
About 50 mg of liver tissue was taken from the control, CCL 4 , BDDP, and PCPE-H groups. Total RNA was extracted according to the manufacturer's instructions using an RNA extraction kit (Sangon Biotech Shanghai Co., Ltd., Shanghai, China). Total RNA was used as the amplification template for reverse transcription amplification of cDNA using a Trans Gen cDNA kit (Biotech Co., Ltd., Beijing, China), and stored at −20 • C. The mRNA primers of different genes were synthesized by Dalian Bao Biological engineering Co., Ltd. (Table 1). The real-time PCR reaction was conducted according to the kit instructions (TransGen Biotech, Beijing, China), and relative mRNA transcription volume was calculated by 2 − Ct [22].

Statistical Analysis
Data were analyzed by one-way ANOVA using SPSS 20.0.0 (IBM, Armonk, NY, USA). All parameters determined in this study are presented as the mean ± standard deviation (ns: p > 0.05; *: p < 0.05; **: p < 0.01). GraphPad Prism version 8.0.1 (GraphPad Software, San Diego, CA, USA) was used to generate graphs with error bars.

Infrared Spectroscopy Analysis
The infrared spectrum results showed that the absorption peak was relatively dense in the band of 2000-900 cm −1 , and the band of 1310-900 cm −1 was the stretching vibration region of a single bond and the bending vibration of some hydrogen-containing groups. 1500-1310 cm −1 mainly represents the bending vibration of C-H. The band of 2000-1500 cm −1 is mainly characterized by carbon skeleton ring respiratory vibration of the benzene ring and stretching vibration of C=N, C=O, and C=C. The bands of PCPE in the region 2000-900 cm −1 are similar to those of kaempferol, quercetin, and myricetin which the IR spectra are dominated by the bands in the region 1800-1000 cm −1 [23]. 3400-3200 cm −1 is mainly O-H stretching vibration, which indicates that PCPE mainly contains flavonoid active components (Figure 1). Many flavonoids such as quercetin and kaempferol have been demonstrated to be the important bioactive constituents of Penthorum Chinense Pursh and possess strong hepatoprotective activity [13].

Statistical Analysis
Data were analyzed by one-way ANOVA using SPSS 20.0.0 (IBM, Armonk, NY, USA). All parameters determined in this study are presented as the mean ± standard deviation (ns: p > 0.05; *: p < 0.05; **: p < 0.01). GraphPad Prism version 8.0.1 (GraphPad Software, San Diego, CA, USA) was used to generate graphs with error bars.

Infrared Spectroscopy Analysis
The infrared spectrum results showed that the absorption peak was relatively dense in the band of 2000-900 cm −1 , and the band of 1310-900 cm −1 was the stretching vibration region of a single bond and the bending vibration of some hydrogen-containing groups. 1500-1310 cm −1 mainly represents the bending vibration of C-H. The band of 2000-1500 cm −1 is mainly characterized by carbon skeleton ring respiratory vibration of the benzene ring and stretching vibration of C=N, C=O, and C=C. The bands of PCPE in the region 2000-900 cm −1 are similar to those of kaempferol, quercetin, and myricetin which the IR spectra are dominated by the bands in the region 1800-1000 cm −1 [23]. 3400~3200 cm −1 is mainly O-H stretching vibration, which indicates that PCPE mainly contains flavonoid active components (Figure 1). Many flavonoids such as quercetin and kaempferol have been demonstrated to be the important bioactive constituents of Penthorum Chinense Pursh and possess strong hepatoprotective activity [13].

Effect of PCPE on Clinical Symptoms of ALI Dogs
The results showed that the improvement rate and effective rate of the PCPE-H group were 62.5% and 87.5%, and were 50% and 62.5% of the PCPE-M group, whereas the PCPE administration in the PCPE-L group does not show improvement in clinical signs ( Table  2).

Effect of PCPE on Clinical Symptoms of ALI Dogs
The results showed that the improvement rate and effective rate of the PCPE-H group were 62.5% and 87.5%, and were 50% and 62.5% of the PCPE-M group, whereas the PCPE administration in the PCPE-L group does not show improvement in clinical signs (Table 2).

Histopathological of Liver Tissues
In the CCl 4 -induced group, canine hepatocytes showed degeneration, swelling, and necrosis, with a disordered arrangement of hepatic cords, a large number of inflammatory cells infiltrated, and the cytoplasm was almost transparent, forming vacuoles ( Figure 2b). As compared with the CCl 4 group the degeneration of canine hepatocytes in the BDDP group was improved, the degree of injury was reduced, and the liver tissues were orderly arranged, but vacuolar degeneration was still observed (Figure 2c). As compared to the CCl 4 -induced group, PCPE reversed the ALI in a dose-dependent (high to low, respectively) Animals 2022, 12, 569 6 of 13 manner by improved hepatocytes degeneration, reduction in cell swelling, and reduction in inflammatory cell infiltration (Figure 2d-f).

Histopathological of Liver Tissues
In the CCl4-induced group, canine hepatocytes showed degeneration, swelling, and necrosis, with a disordered arrangement of hepatic cords, a large number of inflammatory cells infiltrated, and the cytoplasm was almost transparent, forming vacuoles ( Figure 2b). As compared with the CCl4 group the degeneration of canine hepatocytes in the BDDP group was improved, the degree of injury was reduced, and the liver tissues were orderly arranged, but vacuolar degeneration was still observed (Figure 2c). As compared to the CCl4-induced group, PCPE reversed the ALI in a dose-dependent (high to low, respectively) manner by improved hepatocytes degeneration, reduction in cell swelling, and reduction in inflammatory cell infiltration (Figure 2d-f).

Biochemical Analysis of Serum
The effects of CCl4 and PCPE on hepatic functional enzymes (ALT, AST, GGT, and TBA) in serum were quantified on the 3rd, 5th, and 7th day of treatment. The significantly increased indices of ALT, AST, GGT, and TBA were observed in the serum of the CCl4 vs. control. In comparison with CCl4 induced group, PCPE ameliorated and improved the adverse effects by decreasing the levels of ALT, AST, GGT, and TBA in serum (Figure 3).

Biochemical Analysis of Serum
The effects of CCl 4 and PCPE on hepatic functional enzymes (ALT, AST, GGT, and TBA) in serum were quantified on the 3rd, 5th, and 7th day of treatment. The significantly increased indices of ALT, AST, GGT, and TBA were observed in the serum of the CCl 4 vs. control. In comparison with CCl 4 induced group, PCPE ameliorated and improved the adverse effects by decreasing the levels of ALT, AST, GGT, and TBA in serum (Figure 3).

Serum Antioxidant Indexes
Indices of SOD and GSH-Px in the serum were decreased, and indices of MDA and NO were increased in the CCl4 group vs. control group on the 3rd, 5th, and 7th days (p < 0.01). In comparison with the CCl4 group, however, the activity of SOD and GSH-Px were increased and the content of MDA and NO decreased in the BDDP and all PCPE treated groups (p < 0.01). The PCPE-H produced more impact on antioxidant enzymes indices on

Serum Antioxidant Indexes
Indices of SOD and GSH-Px in the serum were decreased, and indices of MDA and NO were increased in the CCl 4 group vs. control group on the 3rd, 5th, and 7th days (p < 0.01). In comparison with the CCl 4 group, however, the activity of SOD and GSH-Px were increased and the content of MDA and NO decreased in the BDDP and all PCPE treated groups (p < 0.01). The PCPE-H produced more impact on antioxidant enzymes indices on the 7th day ( Figure 4).

Serum Antioxidant Indexes
Indices of SOD and GSH-Px in the serum were decreased, and indices of MDA and NO were increased in the CCl4 group vs. control group on the 3rd, 5th, and 7th days (p < 0.01). In comparison with the CCl4 group, however, the activity of SOD and GSH-Px were increased and the content of MDA and NO decreased in the BDDP and all PCPE treated groups (p < 0.01). The PCPE-H produced more impact on antioxidant enzymes indices on the 7th day (Figure 4).

Analysis of Inflammatory Cytokine
Essential serum pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and anti-inflammatory cytokines (IL-10) expressions in CCl 4 -induced liver injury were measured. The serum levels of IL-1β, IL-6, and TNF-α in the CCl 4 group were significantly increased (p < 0.01), and IL-10 were decreased on the 3rd, 5th, and 7th day of treatment compared to those in the control group. After administration of BDDP and PCPE decreased in the levels of pro-inflammatory cytokine and an increase in the level of anti-inflammatory cytokine was observed in CCl 4 -induced liver injury ( Figure 5).

PCPE Effects on Transcription of MAPK/NF-κB
The serum indexes revealed that PCPE-H had a greater influence on improving ALI. For further validation, PCPE effects were explored on signaling pathways through qRT-PCR. In contrast with control, MEKK4, MKK3, p38MAPK, MSK1, and NF-κB p65 genes were overexpressed, whilst IkB was decreased in the CCl 4 -induced group (p < 0.01). However, reciprocal effects were observed in mRNA levels (p < 0.01) by administrating BDDP and PCPE-H against CCl 4 -induced ALI ( Figure 6).
Essential serum pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) and anti-inflammatory cytokines (IL-10) expressions in CCl4-induced liver injury were measured. The serum levels of IL-1β, IL-6, and TNF-α in the CCl4 group were significantly increased (p < 0.01), and IL-10 were decreased on the 3rd, 5th, and 7th day of treatment compared to those in the control group. After administration of BDDP and PCPE decreased in the levels of pro-inflammatory cytokine and an increase in the level of anti-inflammatory cytokine was observed in CCl4-induced liver injury ( Figure 5).

PCPE Effects on Transcription of MAPK/NF-κB
The serum indexes revealed that PCPE-H had a greater influence on improving ALI. For further validation, PCPE effects were explored on signaling pathways through qRT-PCR. In contrast with control, MEKK4, MKK3, p38MAPK, MSK1, and NF-κB p65 genes were overexpressed, whilst IkB was decreased in the CCl4-induced group (p < 0.01). However, reciprocal effects were observed in mRNA levels (p < 0.01) by administrating BDDP and PCPE-H against CCl4-induced ALI ( Figure 6).

Discussion
The pathogenesis of acute or chronic liver injury is governed by oxidative stress in various animal species [24]. The over-generation of reactive oxygen species (ROS) may be induced by various hepato-toxicants including metals, alcohol, or CCl4. CCl4 is commonly used for establishing an experimental model of acute liver injury (ALI) [4], so novel ethnomedicine or allopathic medicine will be investigated. CCl4 is metabolized by the liver and triggers free radical production, such as the trichloromethyl group [25]. The generated free radicals can directly induce lipid peroxidation in the cell membrane and destroy the cell membrane, which is damaged by free radical chain reaction, and the hepatocytes produce oxidative stress, degeneration, hepatocellular injury, and necrosis finally by the induction of ALI [26].

Discussion
The pathogenesis of acute or chronic liver injury is governed by oxidative stress in various animal species [24]. The over-generation of reactive oxygen species (ROS) may be induced by various hepato-toxicants including metals, alcohol, or CCl 4 . CCl 4 is commonly used for establishing an experimental model of acute liver injury (ALI) [4], so novel ethnomedicine or allopathic medicine will be investigated. CCl 4 is metabolized by the liver and triggers free radical production, such as the trichloromethyl group [25]. The generated free radicals can directly induce lipid peroxidation in the cell membrane and destroy the cell membrane, which is damaged by free radical chain reaction, and the hepatocytes produce oxidative stress, degeneration, hepatocellular injury, and necrosis finally by the induction of ALI [26].
Penthorum Chinense Pursh is a traditional Chinese rich with active constituents such as flavonoids, organic acids, and terpenoids, and are commonly used for liver diseases with remarkable curative and low toxic effects [27]. Some studies revealed that 5-hydroxyflavanone-7-O-β-D-glucoside, quercetin, kaempferol, pinocembrin, catechins, and so on are important constituents of Penthorum Chinense Pursh extracts [28,29]. Most constituents of them are flavonoids. We also validated through infrared spectroscopy that flavonoids and polyphenols were rich therapeutic compounds in PCPE and might be involved in the hepatoprotective effect. For instance, kaempferol, quercetin, Pinocembrin-7-O-beta-Dglucoside, and many other flavonoids and phenolic compounds, extracted from Penthorum Chinense Pursh, have been reported that they have strong antioxidant and antiinflammation activities, and have significant liver-protecting effects [30,31].
The present study evaluates the protective effect of PCPE against CCl 4 -induced ALI in dogs and which is linked with the NF-kB and MAPK pathways. Infrared microscopy results demonstrated that flavonoids (biomolecule compounds) in more concentration were found in the PCPE. It is speculated that reversing the effects on ALI such as interaction with liver histology, liver function enzymes, antioxidant enzymes, inflammatory cytokines, and MAPK/NF-κB pathways might be due to flavonoids. Previous reports suggest that flavonoids interacted with inflammatory mediators, and MAPK/NF-κB pathways [32][33][34].
The liver is the main organ for numerous physiological processes, such as macronutrient metabolism, endocrine, and exocrine functions, immune response, growth and development, and the breakdown of xenobiotic compounds [35]. The integrity of the liver tissue structure is a prerequisite to maintaining normal function [36]. Liver functional indicators are a variety of enzymes such as amino transaminases [37]. As a result of liver cells damage due to toxicity, the functional activity has lost the lead to an increase in releasing of biomarkers or enzymes (AST and ALT) into the blood [38]. During the present study, the liver structure is damaged and the level of liver function enzymes was abnormal owing to CCl 4 -mediated injuries, indicating that liver functions were impaired, however, liver function significantly improved with PCPE treatment.
Oxidative stress is considered to be a key factor leading to liver damage. SOD and GSH-Px are important antioxidant enzymes in the body, which can remove important free radicals in the body, inhibit lipid peroxidation and protect liver cells [39]. MDA is the product of lipid peroxidation induced by oxidative stress [40]. NO is the product of the internal nitrogen-free radical and nitrogen-containing small molecule metabolism process, which can reflect the degree of oxidative stress [41]. Our results show that PCPE improved CCl 4 -induced oxidative stress by producing an impact on antioxidant and oxidative stressrelated enzymes (SOD and GSH-Px, MDA, and NO).
Inflammatory reactions occur around the liver tissue in ALI dogs and play a key role in controlling liver disease as previously reported [42]. Pro-inflammatory factors such as IL-1β, IL-6, and TNF-α are important mediators involved in the inflammatory response, and IL-10, a multifunctional negative anti-inflammatory factor, acts as an antagonistic inflammatory mediator [43,44]. IL-1β is a member of the IL-1 family and is a central cytokine in many diseases. IL-1β exerts a pro-inflammatory role in liver disease by interacting with IL-1R and the expression is low in the healthy liver [45]. IL-6 is mainly produced by proinflammatory cells and hepatocytes and has both anti-inflammatory and anti-inflammatory biological effects. IL-6 can promote hepatocyte survival and regeneration in some ALI models [46]. But many studies suggested that IL-6 promoted liver inflammation and aggravated liver damage in CCl4-induced injury [47,48]. TNF-α is primarily produced by activated macrophages and plays a crucial role in activating NF-κB and MAPK pathways, and then results in systemic inflammation. ALI is closely related to the increasing level of TNF-α [49,50]. IL-10 can be produced by lymphocytes and inhibit liver fibrosis [51]. IL-10 has also been reported to play an anti-inflammatory role in acute liver injury. Phytochemical extracted from herbs regulate the release of these inflammation-related cytokines and play an important role in the treatment of disease [52]. We validated that pro-inflammatory factors (IL-1β, IL-6, and TNF-α) were reduced and anti-inflammatory factors were increased after administration of PCPE in challenged (CCl 4 -induced) leading to reversing in ALI.
MAPK signaling transmits upstream signals to downstream response molecules by sequential phosphorylation [53]. MEKK4 is a member of the MEKKK protein kinase family. Phosphorylated MEKK4 activates MKK3, which regulates the expression of MEKK4, and phosphorylated MKK3 activates p38MAPK [54]. Phosphorylated p38MAPK regulates the transcription of downstream genes and plays its biological function, such as regulating the inflammatory response [55]. The activated p38MAPK was transcribed into the nucleus and activated its downstream nuclear protein kinase MSK1, which eventually activated NF-κB [56]. The NF-κB signaling pathway is a key pathway involved in immune and inflammatory responses [57], and IkB is a negative regulatory protein of NF-κB [58]. In the present study, we mainly explored the effect of PCPE on ALI inducing by CCl 4 via MAPK/NF-κB pathway. Our findings indicate that CCl 4 activated MAPK/NF-κB pathway. However, PCPE treatment inhibited the overexpression of MAPK/NF-κB pathway-related genes (MEKK4, MKK3, p38 MAPK, MSK1, and NF-κB) induced by CCl 4 , whereas upregulation of IkB was observed. Therefore, PCPE treatment can reduce the inflammatory response and oxidative damage by suppressing MAPK and NF-κB signaling pathways, thereby improving ALI (Figure 7).

Conclusions
In conclusion, PCPE treatment effectively improved CCl4-induced ALI by increasing anti-inflammatory and by reducing pro-inflammatory factors. This protective effect of PCPE is linked with the reduced oxidative stress and the enhanced oxidant defense systems via the suppression of MAPK/NF-κB Signaling Pathway. Our results suggest that Penthorum Chinese Pursh Extract can be used in veterinary clinical practices as an effective herbal drug for the treatment of acute liver injury.

Conclusions
In conclusion, PCPE treatment effectively improved CCl 4 -induced ALI by increasing anti-inflammatory and by reducing pro-inflammatory factors. This protective effect of PCPE is linked with the reduced oxidative stress and the enhanced oxidant defense systems via the suppression of MAPK/NF-κB Signaling Pathway. Our results suggest that Penthorum Chinese Pursh Extract can be used in veterinary clinical practices as an effective herbal drug for the treatment of acute liver injury.