Recognizing the Benefits of Pre-/Probiotics in Metabolic Syndrome and Type 2 Diabetes Mellitus Considering the Influence of Akkermansia muciniphila as a Key Gut Bacterium

Metabolic syndrome (MetS) and type 2 diabetes mellitus (T2DM) are diseases that can be influenced by the structure of gut microbiota, whose improvement is often neglected in metabolic pathology. This review highlights the following main aspects: the relationship between probiotics/gut microbes with the pathogenesis of MetS, the particular positive roles of Akkermansia muciniphila supplementation in the onset of MetS, and the interaction between dietary polyphenols (prebiotics) with gut microbiota. Therefore, an extensive and in-depth analysis of the often-neglected correlation between gut microbiota and chronic metabolic diseases was conducted, considering that this topic continues to fascinate and stimulate researchers through the discovery of novel strains and their beneficial properties.


Introduction
Metabolic syndrome (MetS) is a combination of risk factors, such as overweight/obesity, hypertension, and imbalances in the metabolism of lipids and carbohydrates. All components of MetS are well-known risk factors when it comes to developing type 2 diabetes mellitus (T2DM), heart diseases, and atherosclerosis. As a consequence of its pathophysiological association with other cardiovascular risks, this chronic condition is one of the main contributors to the prevalence of the disease. As obesity is a precursor of MetS, the maintenance and regulation of MetS will be important in the treatment of obesity by physical activity (e.g., exercises), lifestyle improvements (counseling), caloric restriction menu, reduction of weight by medication, or through weight loss surgery. Strategies such as exercise and behavioral improvement involve firm discipline of the mind and are difficult to follow. In comparison, calorie-limited diets in obese children have shown to be less efficient. The latest pharmacological drugs often determine adverse side-effects and high treatment costs [1].
Several researchers have suggested various nutritional solutions based on probiotic or prebiotic approaches, following a close association between food, gut microbiota, and MetS pathophysiology.
Prebiotics are a peculiar type of dietary fiber with health benefits that not only selectively administer live microbes but are also invoking alterations in the host microbial environment [2]. They can be found in a variety of sources, such are non-digestible oligosaccharides, non-digestible sugars, non-refined wheat, non-refined barley, soy, crude oats, breast milk, and inulin sources (e.g., chicory roots and Jerusalem artichoke) [3]. Prebiotics used as non-digestible food additives have a favorable effect on the host by selectively inducing a small number of colon microorganisms to develop and/or to be enabled [4]. Inulin-based fructose oligomers or galactic oligosaccharides are the majority of prebiotics [5]. Pullulan has long been used for food additives as a possible prebiotic compound [6]. Pullulan is a maltotriosis α-1.6-related polymer and is separated from the Aureobasidium pullulan fungus [7]. It is also considered a non-digestible carbohydrate since it has a high molecular weight and has sluggish hydrolysis of α-amylase and glucoamylase [8]. Pullulan fermented via microbiota has previously been documented to modify the composition of the intestinal microbiota [9]. The FAO and WHO have described probiotics as non-pathogenic living microorganisms, which guarantee the proper health of the host when properly used in foods or food supplements [10].
Probiotics are derived from multiple ways, such as diverse natural habitats, human intestinal microbiota, and food [3,11]. The prophylactic and therapeutic actions of some pre-/probiotics in numerous gut-related disorders are partially mediated via modification or the function of the microbiota. Considering previous definitions, probiotics acts "by contributing to [the host's] intestinal microbial balance" [12] or "by improving the properties of the indigenous microflora" [13]. Taking into account those mentioned so far, in the current consensual form of the definition of probiotics, their effects are considered to be mediated both by microbiota and by other mechanisms. Thus, probiotic microorganisms can function via numerous pathways, including modulation of immune function; interaction with the resident microbiota; host interface; formation of organic acids, enzymes, or other antimicrobial compounds; and improving the quality of the intestinal barrier, etc.
On the other hand, prebiotics are considered to be substrates through which host microorganisms selectively confer a health benefit, their actions including mineral absorption, defense against pathogens, metabolic effects and regulation of satiety, bowel function, immune modulation, etc. The use of pre-/probiotics has been demonstrated by credible and pertinent evaluations of their effectiveness, but it should be mentioned that not all tested products have been validated. The most important target here can be considered the recommendation of these supplements (clearly based on scientific evidence) only by health professionals [14].
Research has shown that the gut microbiota is one of the host's most significant environmental influences and establishes dynamic interactions with the hosts over their entire existence. The metabolic role of intestinal microbiota is also crucial to biochemicals in the body that save energy, produce energy-efficient compounds, and produce vitamins and other essential nutrients [15]. In addition, the intestinal microbiology protects its host from pathogenic inflammation and regulates the innate and acquired immunity [16,17]. Researchers have recently reported imbalances in intestinal microbiota, disease sensitivity, immune deficiencies, and, most specifically, obesity and resistance to insulin [18].
Other studies have shown that the prevalence and treatment of diabetes mellitus (DM), in particular T2DM, requires the use of the host microbiome. Dysbiosis has a clear association between T2D and the gut microbiota (GM) of T2D patients with opportunistic pathogens, particularly the butyrate that generates the bacteria, decreasing the number of beneficial microbes. The incoherence of gut microbiota (GM) is considerable. Improving intestinal health can help control diabetic problems and can determine more DM-related health issues from arising [19,20].
Akkermansia muciniphila (A. muciniphila) is a new highly promising probiotic. It colonizes the mucosal layer in the intestines and modulates the basal metabolism. The association between A. muciniphila and obesity is consistent. A number of animal models and human trials have shown the causal positive effect of A. muciniphila therapy on obesity, this probiotic being an enhancer actor in the metabolism of organism and having considerable potential for treating obesity-related metabolism, as well as related to the treatment including newly medicinal agents or compounds [21,22]. In this regard, a good example are the results of Shang et al., illustrating the beneficial effects of Akkermansia on MetS during the treatment of the disease by fucoidan [21].
The purpose of this in-depth analysis, of a topic that may seem exhausted at first glance, is to provide a very complex and complete overview regarding the relationship between probiotics/gut microbes with the pathogenesis of MetS, the roles of A. muciniphila in the onset of MetS, and the interaction between dietary polyphenols (prebiotics) with gut microbiota. The present paper thus consists of extremely concentrated and useful referenced information for any future works in the field. The reason we selected the current subject was the increasing prevalence of MS and its associated diseases, considering also the fact that numerous therapies are not controlled from the point of view of objectives attainment; therefore, additional measures are needed. Furthermore, supplementation with pre-/probiotics is an easy way to give to the patient another modality to control or at least ameliorate his condition and improve his quality of life.

Methodology
This review involved an exhaustive research of the literature and included the selection of scientific published data addressing the topic of supplementation with pre-/probiotics in MetS and T2DM. To capture all the details relevant to the research, there was no restriction on the publication date of the selected articles, being included both recent and older works. Moreover, for the purpose mentioned above, the most relevant medical and biological data bases (PubMed/MEDLINE, EMBASE, Science Direct, etc.) for articles published until February 2021 were searched, using the representative keywords (mentioned at the beginning of this paper) in the topic or their combinations, in order to find the main data/results/papers in the field. The flow chart describing the selection process of the articles included in this research is presented in Figure 1. Were excluded the publications that were not on the topic approached in this paper and those that were not in English.

Intestinal Microbiota and the Link to MetS
The connection between the composition of intestinal microbiome and metabolic diseases, including obesity and diabetes, was seen in recent studies focused on large-scale sequencing of the 16S rRNA, quantitative real-time PCR (qRT-PCR), in situ fluorescing hybridization (FISH), high-throughput technology pyrosequencing, and deoxy-ribonucleic acid (DNA) bar codes. Researchers demonstrated that obesity may cause the intestinal microbiota composition to change both in mice and people. In contrast with their fat counterparts, lean laboratory animals (i.e., rats, mice and pigs) have a larger amount of Bacteroidetes spp., where Firmicutes are prevalent [23][24][25][26][27]. Therefore, in comparison to lean subjects, a decreased ratio of Bacteroidetes/Firmicutes was identified. There is, however, a lack of coherence between studies. Low microbial gene richness has recently been hypothesized as a good marker for MetS [28,29]. In addition, an abnormal gut microbiota is able to induce sub-acute systemic inflammation, insulin tolerance, and increased risk of CVD through bacterial exposure pathways, namely, bacterial lipopolysaccharides (LPS) [30].
Owing to the mixture of genetic and environmental causes, the prevalence of obesity, diabetes, and MetS is attributed to gastrointestinal microbiota. There are several indications that obesity and related intestinal microbiota metabolic disorders are associated with low-grade systemic inflammation [31,32]. The treatment of metabolic disorders and of obesity-related endotoxemia is important in preventing intestinal microbiota dysbiosis and maintaining the function of the epithelial barrier of the intestine [33]. Metabolic endotoxemia is the pathological disorder attributed to low-grade LPS (endotoxin) plasma elevation from the intestines to the bloodstream [34]. Studies have shown that this endotoxin exacerbates chronic metabolic disease pathogenesis and is common in people with DM, dyslipidemia, insulin tolerance, and obesity in subclinical inflammatory processes [35].

Influence of Intestinal Microbiota Dysbiosis
The cause of dysbiosis is related to the loss of usual functions of the microbiota generating many metabolism disorders [36]. A fat-rich diet can also affect the dysbiosis process, resulting in increased serum lipopolysaccharide (LPS) and dysfunctions of the intestinal barrier [37]. In comparison, dysbiosis can aggravate the still unknown pathogenesis of chronic inflammatory disease [38].
Some bacterial cell components have an impact on the immunomodulatory function of the lymphoid tissue in the increased inflammatory phase. Researchers suggest that the translocation and signaling of antigens via the intestinal mucosal barrier are activated by cell membrane components, including peptidoglycans and LPS. A pro-inflammatory function is accomplished by binding LPS and peptidoglycan to toll-like receptor 2 (TLR4) and by activating the immune system in a cascade reaction with the oligomerization domains of nuclear oligotides (NOD) [39,40].
The LPS serum levels are double in obese, diabetic, or extremely fat people, as a result of reduced intestinal barrier resilience, increased development of chylomicron during the digestive phase, and a reduction in the function of the alkaline phosphatasis, which causes the cleavage of this endotoxin. This repeated sensitivity of MetS to serum LPS has also caused MetS association with the innate immune system, assisted by the use of the protein LPS (LBP) and CD14 co-receptor [41,42].
In the intestinal microbiota and the innate immune system, MetS production is interactive. The latest actions are aimed at creating a specific diet to reconstruct and/or strengthen the immune system underlying the intestinal homeostasis of microbiota [43]. In addition, the unique strain properties of gut microbiota have been shown by laboratory models for the attenuation of some chronic infectious immune responses [43].
While the bowel microbiota of adults is constant, food, genotypical/epigenetic combination and immune-metabolic function may determine adjustments. The signaling pathways between the host and microbiome, including different groups of ligands, are used as essential effectors for modification, as stated by Moran and Shanahan in 2014 [44,45]. β-cell syntheses, which control insulin and glucose homeostasis, are enhanced by the involvement of inflammatory biomarkers in diabetes-induced oxidative and terminal stresses [46].
If the structure of the intestinal microbiota becomes more intricate and contagious, more modifications are seen in the macrophage M2 to M1 adiposis environment, which can contribute to the development of MetS [47].

Influence of Dietary Composition
Although the intestinal microbiome responds to major caloric intake changes, numerous experiments suggest that it is most vulnerable to dietary composition [48,49]. In a human trial, volunteers had either a plant-based (cereal, fruit, legumes) or animal-based (meats, eggs, cheeses) diet for five consecutive days. After these five days, their microbial populations underwent major behavioral shifts. Participants whose diet focused on animal products witnessed a bloom of bile-resistant microbes (Alistipes spp., Bilophila spp., and Bacteroides spp.) and a reduction of fiber-fermented bacteria [48]. Additional experiments have shown microbial exposure to seeds, fiber forms, and food contaminants in dietary fats [50][51][52][53].
Particularly interesting are dietary fats, since certain acids have antimicrobial activity, but this feature is calculated by the number of carbons and double C=C-bonds, their position, and orientation [54]. Caesar also found that the proinflammatory Bilophila wadsworthia has bloomed in rats who have been fed a lard diet, while Lactobacillus and A. muciniphila have bloomed in the diet based on fish oil [55]. An increase in the number of A. muciniphila was adversely connected to obesity, T2DM with medication, and elevated BP [56][57][58].
Recently, in overweight/obese insulin-resistant people, the first clinical trial involving oral A. muciniphila supplementation was carried out. The randomized, double-blind combination study was conducted for 3 months and revealed an increase in insulin sensibility and reduction in insulinemia and overall plasma cholesterol. Therefore, it may also be part of the solution, even though the interaction between the diet and the microbiome implies the progression of obesity [59].
An impaired microbiota is further involved in promoting the risk of cardiovascular disease. Studies performed on antibiotic and germ-free mice indicate an important role of gut microbiota in transforming nutrient phosphatidylcholine in trimethylamine N-oxide molecule (TMAO) [60,61]. In this direction, microbes cleave dietary choline through the hepatic flavin mono oxygenize 3 (FMO3) to form TMAO from animal products, which is oxidized, in return, by the liver. The levels of circulating TMAO in patients with atherosclerosis were substantially higher relative to the stable controls [61]. In recent human research, vegan and omnivores who have been confronted with a supplementing choline diet have demonstrated a dose-dependent rise in TMAO in the circulation. After treatment with large spectral antibiotics, the prothrombotic phenotype is absolutely removed, which clearly indicates that the microbiome has an important role in this phenomenon [62].

Influence of Inflammation
Although a consistently low inflammation is not a distinctive cause for MetS, it is a recognized element in the etiopathology of obesity/insulin resistance and is thus closely related to MetS metabolism. The important function of intestinal permeability in persistent low-grade inflammation renders the microbiota as a crucial part of the inflammation in metabolic deficiencies.
Inflammation represents a series of responses of the vascularized tissue to damage or infection. This is a protective survival function, but prolonged sensitivity to stimuli and immune movement in cells can be harmful. The first study of chronic low-degree inflammation with obesity and insulin resistance was documented by Hotamisligl and others in 1993. Rotary adipose tissue tolerance to insulin was achieved by TNF-α overexpression [63,64]. This survey was eventually extended to include obese people, who had elevated TNF-α mRNA as opposed to the controls in obese adipose tissue. Scientists found that metabolic cells, such as adipocytes and hepatocytes, had closely related immune systems and vessels. This proximity provides a continuous communication between the immune response and metabolism [65,66].
In fact, multiple studies found that adipose tissue in obese individuals has increased proinflammatory markers (with expanded adipocytes in mice and humans with macrophage and dead crown like structures of phagocytic macrophages) [67,68].
Inflammatory incidents contribute to an altered metabolism, frequently determined by the experimental usage of high-fat diets; nevertheless, considerable attempts are being made to clarify which causes, aside from a high-fat diet (HFD), are triggers of inflammation [69].
The function of the intracellular endocannabinoid system (ECB) is another interesting gut-centered process that can contribute to low-grade inflammatory dysmetabolism. Obesity increased the ECB levels in adipose tissue and plasma and a strong ECB synthesis stimulator for the bacterial LPS was found in this condition [70].
Pharmaceutical or genetic inhibition of the CB1 receptor cannabinoid protects against obesity, hepatic steatosis, and low-grade inflammation. In the context of monitoring the subsequent rat experiments, investigators concluded that the intestinal microbiome ECB behavior in the colon and adipose tissue (comparing the germ-free to standard mouse and analyzing the genetically engineered microbiome) was reported to be changed. The alleged role of the ECB system in healthy persons has yet to be known, but bacterial LPS can be induced and contributes to metabolic dysfunction [71].

Effects of Pre-/Probiotic Administration in MetS
While some clinical trials confirm the prediction that MetS is positively affected by probiotics and/or prebiotics, other experiments have shown contradictory findings, Table 1 summarizing them.

Form of the Product; Species/Active Substance; Period of Administration, Dose of Pre/Probiotic
Effects/Actions Ref.
From its inception until 4 July 2019, in PubMed and Scopus, a qualitative systematic review was performed, according to the Cochrane approach, as well as a thorough literature search for randomized, controlled experiments. Nine clinical trials were finally reviewed according to our inclusion criteria, equal to six randomized control trials (RCTs).

Effects of Probiotics Alone
In some trials, the use of probiotics in patients with MetS has increased the index of body fat, BP, lipid profile, and glucose metabolism. In inflammatory biomarkers, probiotics also have a beneficial effect on molecule 1 (sVCAM-1), 6 (IL-6), α factor of necrosis (TNF-α), endothelial vascular factor of growth (VEGF), and thrombomodulin. The admission of probiotic compounds in patients with MetS leads to discreet changes in some clinical features of MetS and a decrease in inflammatory biomarkers, considering the diversity of the published reports [102].
In a Swedish analysis of the HFD-fed mice supplemented with L. plantarum, the lack of any reaction, specifically to the intravenous glucose-tolerance test, was related to the administration route that could not induce glucagon-like peptide-1 and release insulin. After collection from 426 strains, L. plantarum was used in a Korean sample as well [103].
The decline in insulin resistance parameters was associated to the cell reports of increased insulin signaling and inflammatory genes. L. casei interference improved the concentration of Lactobacillus and Bifidobacterium and decreased Clostridium in hyper insulinemic fructose-fed mice. The reduction of GLP-2 induced by Bacteroides fragilis was related to decreased insulinemia, while performing oral glucose tolerance tests (OGTT) [104]; moreover, some advantages were also found in liver function genes and gene expressions.
L. coryniformis administration with HFD-fed mice has caused vascular benefits and could be correlated with improvements in the composition of microbiota and intestinal permeability with reduced endotoxemia [105]. In research conducted in Taiwan, the use of lyophilized and "live" L. reuteri supplements increased Lactobacillus and lowered the abundance of pathogens, which helped improve the intestinal barrier. Weight gain, lipid profile change, hepatic steatosis, as well as inflammation and insulin resistance-related gene expressions decreased [106]. L. reuteri has also been used with the Aryl hydrocarbon receptor antimicrobial agonist MS in a French analysis. The addition improves glucose metabolism and liver alanine transaminase. Added synthesis increased glucose and hepatic alanine transaminase levels. In a Korean published paper, HFD-fed mice to whom L. sakei was administered as well, gained both weight and epididymal fat mass, by decreasing the inflammation and increasing the intestinal barrier (increased genetic expressions of tight-joint protein) [107]. Another two experiments in diabetic mice demonstrated similar decreases in insulin tolerance, TNF-α, and IL-6 at a dosage of 10 9 CFU pioglitazone and separate doses of L. casei [108]. The increased concentration of Lactobacillus and Bifidobacterium and SCFA has been linked to a potential rise in glucose homeostasis. Traditionally, probiotic activity stopped pancreatic islets from degeneration and/or regeneration.
An analysis comparing metformin, vildagliptin, and three L. rhamnosus strains (GG, MTCC5690, MTCC5689) increased the insulin tolerance curve areas and OGTT, with the exception of the HFD-MTCC5690 treatment [109]. Probiotics enhanced bowel permeability, increased adiponectin, and reduced inflammatory markers. Metabolic advantages gained through probiotics are similar to metformin and vildagliptin.
An Indian study on diabetic mice revealed greater results on the glucose metabolism, lipid profiles, and oxidation stress markers with L. rhamnosus NCDC17 compared to L. rhamnosus GG supplements. Moreover, adiponectin, pro-glucagon, and prohormone genes (TNF-α and IL-6) were found as being inflamed [110]. HFD-fed mice received Lactobacillus rhamnosus in order to minimize the abundance of Bilophila wadsworthy associated with MS [111]. Additionally, the B. wadsworthia increased in accordance with the effects of glucose metabolism, but the probiotic only enhanced the implications of HFD in the event that these bacteria were in high abundance.
In a Japanese study with L. gasseri in fermented milk, the complementation increased the production of insulin by reducing pancreatic/systemic inflammation [112]. L. paracasei was tested in a study for the advantages of glucose and lipid metabolism in obese rats and for many indicators of renal function and inflammation [113]. A research showing a decline in weight gain, visceral fat accumulation, and inflammation in combination with an increase in glucose and lipids studied the role of sterilized B. longum in obese mouse [114]. By comparing B. lactis and B. longum supplements, improved productivity of the former in terms of acetate and metabolic benefits was revealed [115]. For two doses of B. breve in HFD-induced mice, weight and visceral fat were decreased and genetic expressions decreased [116]. The authors also suggested that the probiotic could enhance healthy commensal bacteria to prevent endotoxemia and inflammation in an experiment with B. animalis [117]. The benefits of C. butyricum supplementation were complemented by increased intestinal and antimicrobial effects, supplementing glucose and lipid metabolism [118].

Effects of Associated Pre-/Probiotics and Synbiotics
Four studies have compared pro-/prebiotics and synbiotics (which are combination between pro-and prebiotics). Many of these have been xylo-oligosaccharide (XOS), inulin, and fructo-oligosaccharide and prebiotics (FOS). First, dysbiosis and inflammation were assessed in rats that were obese, with improved synbiotic results [119]. The groups treated also reduced the Firmicutes spp.-to-LPS ratio and raised the concentration of Bifidobacteria spp. in the prebiotic culture. The investigators concluded that prenatal, probiotic, and synbiotic products contribute to a decrease in endotoxemia and inflammation. The second research study investigated the link between cognitive function, L. paracasei, XOS, and the gut-brain axis in obese rats, which are shielded against insulin [120]. The glucose and lipid metabolism improved with prebiotics, probiotics, and synbiotic interventions, but the adiposity decreased with the XOS and synbiotic. Both products were beneficial, improving brain mitochondrial function and hippocampal plasticity, decreasing also the microglial activation. The third research study explored the association between psychiatric disorders and oxidative stress following addition or mixture of inulin or L. plantarum [121].
All therapy improves oxidative stress and psychoactive effects (depressive and anxietylike behaviors). During the fourth study, in which HFD-fed mice were given L. paracasei, FOS, or their combination, the impacts on NAFLD were assessed [122]. Benefits during intraperitoneal GTT were considered as follows: increased gut barriers, decreasing LPS levels, and successful insulin signaling pathways were found in all of the treated populations. Steatosis improved, as well as dyslipidemia and inflammatory disorders.

Additional Results Obtained with Probiotics of Various Strains
In one study, Lactobacillus spp. and Bifidobacterium spp. were combined, and two other Lactobacillus spp. plus one Bifidobacterium spp. strains were added [123,124]; the first two mediated beneficial modifications in microbiota structure, bodily adiposity, insulin tolerance, and dyslipidemia in HFD-induced obese mouse. In comparison to the isolated effect of each probiotic on the composition, B. animalis had the most pronounced preventive effect on the structure [123]. Another Brazilian trial demonstrated the regulation of the composition of microbiota and increased intestinal permeability, endotoxemia, and inflammation in a mixture of three probiotics. The treatment also included a drop in insulin and leptin hypothalamic tolerance, which influenced the eating pattern (reduced food intake and weight gain) [124].

The Roles of A. muciniphila in the Onset of Metabolic Syndrome
The only Gram-negative emblematic Verrucomicrobia that is widespread in human intestinal mucosa is A. muciniphila [125]. Gene sequence analyses revealed that there are multiple gene candidates for mucin encoding and the single chromosome is contained in 2176 genes with a 55.8% GC content after the MucT type strain of A. muciniphila, as reported by Derrien (ATCC BAA-835 1/4 CIP107961T) [126,127]. This non-motile, ovalformed microorganism is purely anaerobic and with chemical organotrophic material that can withstand low levels of oxygen. A. muciniphila may create mucin-degrading enzymes and use mucin in the mucosal layer of the epithelium as a source of carbon and nitrogen.
A. muciniphila splits these compounds into acetic and propionic compounds, releasing sulfate [128,129]. A. muciniphila also is 3 to 5% of the total gut microbiome population in healthy adult humans based on an analysis of its distinctive 16SrRNA signature, although this amount differs by several factors. In stable human beings, A. muciniphila has been closely linked to age. Its colonization starts at a young age and ranges between 5.0 and 8.8 log cells/g in a year equivalent to the adult stage but decreases in the elderly [130,131]. In comparison, in patients with metabolic disorders, A. muciniphila and a mucosal pathology varied, and the incidence of appendicitis and IBD was reversely associated with this [132]. In addition, a negative association of intestinal A. muciniphila with diabetes, obesity, and others MetS has been shown [133].
Dual control of A. muciniphila and metabolic disorders has demonstrated that both A. muciniphila excess and supplementation can have an effect on the host body. The anatomy of A. muciniphila can also be affected. The dissemination of A. muciniphila through early vancomycin therapy to the early intestinal colonization could help regulate the progression of autoimmune diabetes [134]. In an initial analysis, A. muciniphila was recognized as a potential new therapeutic agent for obese patients. Most studies have shown the advantages of using A. muciniphila in metabolic and obesity disorders for prevention and progress. T2DM is characterized by a lower A. muciniphila abundance, low inflammation, and an intestinal permeability disease [135]. The degree of A. muciniphila development can be used to determine the metabolic state of the body, for instance glucose homeostasis, serum lipids, and human adipocyte distribution. The reason for associating A. muciniphila with the production of obesity is not yet entirely explained. We have therefore analyzed the most recent studies into A. muciniphila's function in obesity and learned more about its effects on the distinctive changes in the expression of pathways in metabolic homeostasis [136].

A. muciniphila and Obesity: Evidence from Mouse Models
All of the previous studies found a correlation between the caloric intake of A. muciniphila and its abundance. Prebiotic administration in high-fat diet mice eliminated the metabolic endotoxemia that characterizes the compromised MetS present among obese participants, decreased the overall fat mass, and decreased body weight [137]. These findings have in fact been strongly linked to an abundance of A. muciniphila [138]. The reduction of A. muciniphila and inflammatory markers were crucial in all circulatory parameters (i.e., glucose, insulin, leptin, and triglycerides), with 118 of the 13 genes implicated in the fatty oxidation, production, and oxidation of positive ties, whereas Bifidobacterium spp. were important. Three inflammatory features, leptin, and only two genes involved in the oxidation of fatty compounds are positively and negatively related.
Schneeberger et al. documented the inversely related levels of A. muciniphila in mice with inflammatory markers, lipid synthesis and insulin tolerance, cardiovascular risk, and adiposity markers in their plasma. After six consecutive weeks of HFD administration, the main effects were body weight gain and adiposity in mice. The results therefore suggest that an HFD particularly affects the gut bacteria and show that the abundance of A. muciniphila is decreasing steadily with sustained dietary care in mice. This bacterium also reduces, showing a causal impact, the disease development before the initiation of metabolic changes [139]. In comparison, the abundance of A. muciniphila increases and decreases in mice fed a diet rich in fish oil and lard, with greater regulation of the intestinal barrier system and less inflammatory tissue, which can be converted into germless receiving mice [55]. Other authors also found that the gut barrier dysfunction, weight, and fat gain of HFD-fed mice can be decreased at the same time with A. muciniphila [140,141]. The relation between age and A. muciniphila was finally identified in mice, as the intestinal level of the bacterium is lower in older mice. An HFD improves the adipose tissue and intestinal microbiological composition considerably compared to aging [139].

A. muciniphila and Obesity: Evidence from Human Studies
Emerging research assessed the correlation between A. muciniphila intestinal abundance and human body weight. There are proofs that these two variables have an opposite correlation [142,143]. The numbers of Bifidobacterium spp. and A. muciniphila and the number of Staphylococcus spp., Enterobacteriaceae spp., and Escherichia coli were found to decrease in overweight pregnancy. They were analyzed by quantitative real-time PCR for their gut microbiota composition. The increase in overall bacteria and Staphylococcus was linked in the whole population with increased plasma cholesterol levels, while the increased amount of Bacteroidites spp. was associated with an increased level of HDL and folic acid [144].
Twenty overweight or obese children and 20 average regular weight children aged 4-5 years were evaluated by Karlsson et. al. Interestingly, in obese/oversized infants, the A. muciniphila levels have decreased dramatically, whereas in the same class, the Gramnegative Enterobacteriaceae concentrations were significantly higher. Bifidobacterium levels in obese/overweight children were inversely linked to alanine aminotransferase (ALT) [145]. Since diabetes and overweight are linked to increased intestinal permeability and low inflammation, endotoxemia caused by LPS is considered as one of the causative agents of obesity-related metabolic disorders. An in vitro observation that confirms the epithelial barrier role of A. muciniphila may provide a working hypothesis to streamline in vivo proof that links decreased fecal A. muciniphila with diabetes and obesity rate. This could reveal a mechanism for protecting HFD obese mice from bacterial LPS endotoxemia. No relationship was currently assessed between A. muciniphila and hypothalamic food intake regulation markers. According to all data, it can be concluded that A. muciniphila influences the reaction of humans in terms of improving inflammation, insulin resistance, and glycemia on their diet of caloric constraint [146].

A. muciniphila Medicinal Role in the Treatment of Metabolic Conditions
The study of A. muciniphila has contributed to a better understanding of its possible therapeutic effects and actions in determining certain diseases and metabolic disorders. A. muciniphila, in particular, tends to be involved in the development of amine butyrate and in the propionate extracellular pool. It is also important in the development of sulfide hydrogen, which can be anti-inflammatory and a powerful antioxidant [147,148]. The involvement of A. muciniphila in metabolism enhances prebiotic intake. Both studies performed on animals with A. muciniphila revealed that it decreases body weight and fat mass rise, hepatic steatosis, inflammation, cholesterol, and atherosclerosis; it also enhances insulin sensitivity and restores intestinal barrier function by influencing various factors, such as the thickness of the mucosal membrane, close attachment proteins, antimicrobial peptides, and immunity. A. muciniphila works in particular on the immunomodulatory involvement of a special protein called Amuc 1100 [149].
Ottman et al. recently documented the increase of glucose resistance and a decline in body weight and fat gain in mice fed an HFD relative to the untreated mice with refined recombinant Amuc 1100 protein. The critical role of A. muciniphila in intestinal health, particularly in metabolic immunomodulation, is evident in this scenario. In order to increase their therapeutic application to gastrointestinal disease, future studies will also need to accentuate the main role of this microorganism and of its proteins in metabolic regulation and immune modulation [150].
The clinical studies focused on the obesity implying A. muciniphila and its associated biomarkers are presented in Table 2.  There was a strong decline in the concentration of the mucin-degrading bacterium A. muciniphila in prediabetes. [154] 49/overweight + obese/12 weeks Calorie restriction for 6 weeks The large amounts of A. muciniphila increased the distribution of fasting plasma glucose, plasma triglycerides, and body fat.  For liver dysfunction and inflammation, A. muciniphila decreased body weight and decreased the levels of the related blood markers, although the overall composition of the gut microbiota remained unchanged. [59]

21/alcoholic steatohepatitis 16/healthy controls Observation
The concentration of fecal A. muciniphila vs. healthy controls (indirectly related to the incidence of hepatic disease) was diminished in ASH patients. [162] 13/overweight adults/7 weeks Interventional, fasting (1 week), followed by probiotic intake (6 weeks) Samples: feces, T1 = before fasting, T2 = during fasting, T3 = 6 weeks after probiotic intervention In comparison to fasting (T2), the concentration of A. muciniphila was higher before fasting (T1) than after probiotic action (T3) [137] 3c/normal weight 3/morbid obesity 3/after gastric bypass surgery Interventional, gastric bypass Reduced quantity of A. muciniphila in obese subjects (who obtained an elevated quantity of A. muciniphila after gastric bypass) vs. average weight subjects [163]  A summary of the beneficial effects of A. muciniphila treatment on MetS conditions is schematized in Figure 2.

The Interaction between Dietary Polyphenols (Prebiotics) with Gut Microbiota
Dietary polyphenols are considered naturally existing substances in plants or obtained from foods (i.e., cereals, coffee, fruit, tea, vegetables, and wine [167][168][169]. It is estimated that the small intestine consumes just 5-10% of the gross intake of polyphenol. Residual polyphenols, along with conjugates excreted into bile in the intestinal lumen, may accumulate in large intestinal lumps to a millimolar stage, where they are subjected to the enzymatic activity of the intestinal microbial group [170]. The gut microbiota and polyphenols have two major complementary relationships. On the other hand, after ingestion, dietary polyphenols are subject to a dynamic metabolism, associated with human and microbial enzymes, which contributes to the production of a broad variety of circulating and excreted metabolites and catabolic items. Polyphenols and their metabolites may on the contrary affect and cause a modification of the structure of gut microbiota via numerous interactions. Some studies support the possibility of modifying and producing variations in the populations of microflora by providing prebiotic effects and anti-pathogenic intestinal flora after dietary phenolic substances reach the gut microbiota, along with aromatic metabolites produced [165][166][167]. Human beings regard the biochemical transformation of dietary polyphenols as a significant concern. A minimal volume of dietary polyphenols is consumed by the small intestine, often after conjugation reactions such as de-glycosylation [166,170]. After absorption into the small bowel, polyphenolic compounds of lower complexity can undergo biotransformation in the enterocyte and then in the liver cell, by step I (oxidation, reduction and hydrolyses). These transformations produce a metabolite of water-soluble conjugates (glucuronide, sulfates, and methyl derivatives) that are rapidly released into the systemic circulation for subsequent organ drainage and urine excretion. The polyphenolic backbone of 90-95% unabsorbed polyphenols comprises colonic bacterial enzymes in the vast intestinal system that manufactures metabolites, which results in various physiological effects [171].
Colonic microflora could turn polyphenols into bioactive compounds that could affect the intestinal ecology and human health. The recommended concentrations will alter the composition of the gut microflora by inhibiting particular bacterial groups, as studies in humans and animal have revealed. Others will prosper in the open niche of the ecosystem [172].
Besides processing the derived food, the gut microbiota may perform a variety of bio-transformations of the polyphenols that enter the colon and influence their intake and bio disposability [172]. Specifically, some phenols in the metabolism of Clostridium spp. and Eubacterium spp. were reported to include isoflavone (daytzine), flavonol (quercetin and kaempferol), flavanone (naringenin and iso-xanthumol), and flavan-3 oil (catechin and epicatechin) [173]. Polyphenol colonic fermentation produces a wide range of biotransformation ingredients, but most of them are derived from phenyl, phenyl propionic, phenyl-butyric, valerical, valerolactone, phloroglucinol, and di-benzo-pyran urolithin A or urolithin B [174]. Microbiota degrade the parent polyphenolics in a variety of products, including phenolic acids such as 3-hydroxyphenyl acid, from flavanol routines synthesis (found in tomatoes, for example). The health benefits of these indirect and final materials arise with the assumption that certain bioavailability factors may be clarified for many biological effects traditionally linked to polyphenols (relative to that of the parent compounds) [175]. Phenolic acids can be found both in the plasma and urine after a meal, but some are activated by mammalian enzyme mechanisms (for example protein catabolism), which make it difficult to understand the plasma and urine levels of phenolic acid. A greater inhibition of anti-platelet aggregation has been shown by 3,4-dihydroxy (3,4DH-PAA), one of the key intermedial metabolites of rutile-catabatic acid [176], as well as of secretion of monocytes of the proinflammatory cytokines TNF-a and IL-6 [177] than by the parent compound. It has also been demonstrated that phenolic acids suppress protein glycation [178]. However, most phenolic acid bioactivity research experiments are still based on in vitro models and more in vivo evidence is needed.
The absorption rate of polyphenol is very low and up to 90% of these substances linger in the colon [179]. They are metabolized through bacterial esterase, glucosidase, demethylation, dihydroxylation, and decarboxylation processes, resulting in smaller metabolites, several of which can be absorbed by the intestinal mucosa, such as phenolic acids and short-chain fatty acids. It is important to know that the microbial bioconversion potential of each organism influences and impacts their bioavailability on the final metabolites produced. In reality, since individuals have their own unique intestinal microbiota synthetic signature that can permit a fingerprint comparison, it can modulate the effect of polyphenol on host welfare by human intestinal microbiota composition [173]. On the other hand, the modulating microbiota of the intestinal ecosystem can be influenced by polyphenols and their metabolites. Certain phenolic compounds with bacteriostatic or bactericidal behavior were considered potential antimicrobial agents. In addition, they can also serve as inhibitors of infection-causing bacteria within intestinal and urinary tract cells, suggesting that such phenolic compounds are capable of being used as antimicrobial agents against human pathogens [180].

Polyphenol Transformation via Intestinal Microbiota
Polyphenols are not well absorbed by GI proximity and tissue responses to highmolecular PPs are now impaired by the metabolism of PP-rich food (such as tea, wine, chocolate, and fruit) [181]. Glycosidic bond cleavage and heterocyclic column breakdown, for example, are related to the biotransformation of polyphenols (PPs) (e.g., anthocyanins) via GM [182]. This biotransformation could be accomplished by different reactions, such as oxidation, hydroxylating, dehydrogenation, decarboxylation, isomerization, glycosylation, methylating, etc. Buddleoside flavonoids were transformed into aglycon acetins by human intestinal microflora, resulting in the transformation of metabolites into methylated and hydroxylated by-products. This research has defined the ability to transform flavonoids extensively, by just four bacterial species, such as Escherichia sp. 4, Escherichia sp. 34, Enterococcus sp. 45, and then Bacillum sp. 46 [183].
It can be concluded that polyphenol degradation metabolites are predominantly responsible for the host's health benefits based on the available evidence.

Modulatory Impact of Polyphenols on Intestinal Microbiota
A proper diet, which includes fruits and vegetables, leads to achieving the best health. Polyphenols (PPs), owing to the potent antioxidant role, have positive health effects, and are associated with fruit and vegetables [187]. The improvements in the bioavailability of PPs and their metabolites are also influencing the structure of the gut microbiota. Considering the data from in vitro studies, both the animal and human trials show, however, that selected PPs could serve as "prebiotics" and modify the ecology of the intestinal microbiota, thereby affecting host health. PPs also play a vital role in providing health outcomes, such as weight loss by GM change and host/microbe interactions [188]. A recent study by Sun et al. found that tea PPs obtained from the samples Bifidobacterium spp., Lactobacillus spp., and Enterococcus spp. have a significant impact on the gut microbiome [189]. The collections have increased SCFA efficacy and minimized the distribution of Prevotella, Bacteroides, and Clostridium histolyticum. In a study that shows the effect of black tea and red wine/grape extract on human gut microbiota, Kemperman et al. used the stimulated intestinal microbial environment, SHIME [190]. According to their study, the growth of Bifidobacteria, B. coccoides, Victivallis, and Anaeraeroglobus was improved by Klebsiella spp., Enterococci spp., and Akkermansia spp. simultaneously. The red wine/grape extract also encouraged the production of Alistipes spp., Cloacibacillus spp., Victivallis spp., and Akkermansia spp., and reduced the development of Bacterioidites spp., Bifidobacterium spp., B. coccoides, Subdoligranulum spp., and Anaeroblobus spp. It has been shown that red wine extract fermentation decreases the in vitro growing of Clostridium histolyticum, a harmful bacterial species in GM [191]. Similar findings were presented in a study carried out with human volunteers and in relation to Clostridium histolyticum development accompanied by red wine consumption [192]. On the other hand, Cueva and others note that the bacterial growth of the intestines can be greatly impaired by in vitro fermentation of the grape-derived flavan-3-ols [193].

Benefits of Polyphenols in MetS Patients
Many studies have shown that fruit-containing PPs play an important role in reducing the complications related to obesity [194][195][196]. An increasing part of the literature also points to a role of PPs in defending against diabetes and related complications by genetically modified organisms. The high prevalence of intestinal Bacteroidetes due to the improved potential for glycan degradation is caused by a PP-rich diet. Researchers thus hypothesized that microbiota modulation may contribute to the anti-obesity and antidiabetic mechanism through polyphenols [197]. The administration of polyphenol-rich cranberry extract (CE) avoided a high fructose-high sucrose dietary weight gain and visceral obesity development in mice. CE also decreased the production of triglycerides and prevented liver inflammation, thus improving the sensibility to insulin and avoiding the development of NAFLD. Furthermore, scientists observed that the positive outcomes of CE therapy are related to increasing Akkermansia's abundance [198]. The effect of dietary supplements with grape polyphenols (i.e., a decline in the ratio of Firmicutes to Bacteroidetes) was substantially modulated in HFD-fed mice [199]. The GP supplementation has also stimulated A. muciniphila development and protected these improvements against the harmful effects of a high-fat diet [200]. The preventive impact against obesity and related risk factors has been identified with procyanidin supplement (PS) intake. PS treatment reduced weight gain, improved dyslipidemia, and reduced energy consumption. The positive influence of PS on obesity was also beneficial related to the effect of genetically modified modulation. The 16s gene sequencing RNA study showed that PS administration significantly increased Bacteroidetes β-diversity, intestinal microbiota, and the Firmicutes-Bacteroidetes ratio. Even an increase is considered to describe obesity-driven dysbiosis in Firmicutes/Bacteroidetes. In addition, studies suggest that an increased pathogenesis and the progression of obesity and T2DM have also been linked with Lachnospiraceae spp. [201].
Everard highlighted the opportunity to improve metabolic symptoms by supplementing with Akkermansia spp. as a probiotic for LPS prevention and intestinal permeability reduction [141]. Li and his colleagues found sinapine polyphenol to alter the intestinal microbiota in high-fat diet-induced mice in preventing NAFLD. It is a chronic low-grade inflammatory liver condition. The administration of Sinapine reduced the ratio of Firmicutes to Bacteroidetes and increased the abundance of Lactobacillaceae, Akkermansiaceae, and Blautia. Moreover, Sinapine stimulated G protein-coupled upregulation 43 (GPR43) of short-chain fatty acid (SCFA) in order to suppress the causes of inflammation [202][203][204]. A recent research study also found that PPs decreased microbiota dysbiosis by scavenging intestinal reactive oxygen species (ROS) through gut metabolism. Grape PPs suppressed the fat-driven generation of intestinal ROS and thereby decreased metabolic syndrome mediated by intestinal dysbiosis. Grape PPs stimulated healthy anaerobic intestinal bacteria, including A. muciniphila, which was linked with increased human metabolism [205]. These results revealed that dietary polyphenols may eradicate metabolic diseases by modulating intestinal microbiota positively.

Triggering A. muciniphila with Dietary Polyphenols
An increasing abundance of A. muciniphila, for instance, was correlated with antidiabetic effects of metformin in gut microbiota of obese rats in which obesity was induced through diet (DIO mice) [206,207].
A recent study has demonstrated that regular administration of polyphenol-rich cranberry extract (CE), orally, stopped weight gain over 8 weeks and enhanced many characteristics of metabolic syndrome, along with a major increase in Akkermansia's abundance in DIO-mice intestinal microbiota [198]. That was the first confirmation that a fruit extract rich in polyphenol rich from fruits has a prebiotic effect on Akkermansia, which enhances the metabolic condition. These results have been supported by a more recent study on grape extracts, also being correlated with the prebiotic effect on Akkermansia, in increasing the metabolism in DIO mice [200]. In line with those observations, in addition to butter fat, the diet of mice was supplemented with California table grape powder, leading to lower adiposity and lipogenesis in conjunction with a higher trend in Akkermansia in intestinal microbiota [208]. Since both cranberries and grapes contain significant pro-anthocyanidin concentrations (PACs, also called condensed tannins), the unique class of polyphenols in this prebiotic operation can be considered of special significance. Cranberry PACs have also previously been correlated with increasing mucus production in mice and can provide Akkermansia with sufficient trophic resources [209].
Together with a propensity towards a growing degree of Akkermansia in gut microbiota, two new experiments have confirmed the benefits of administering flavonol-quercetin to obese rats [210]. Another study found that Akkermansia spp. was present in stool samples of healthy participants eating pomegranate extract (a rich source of ellagitannins) [211]; this new research being especially important, not just because it shows that human gut microbiota often has big effects on Akkermansia spp. when polyphenol rich extracts is called for, but also because healthy persons can benefit from the polyphenols that influence Akkermansia spp., which has prebiotic effects.
Interestingly enough, Roopchand et al. reported the colonic or jejunal expression of Muc2 mRNA after using Concord grape extract, type B PCs, and other polyphenols, which suggests that the Akkermansia niche has a direct effect. These findings, namely that polyphenols in grape juice and red wine increase Akkermansia spp.'s abundance, supported the hypothesis when applied to the in vitro intestine model [190].
Several clinical published data on Akkermansia spp. have shown an improvement in dietary obesity and are linked to a decline in weight gain, obesity, and increased glucose tolerance [206]. Live A. muciniphila administration has reversed obesity and metabolism by reducing adiposity, inflammation signs, insulin tolerance, and strengthening gut barrier in HFD mice [141]. Recently, the introduction of capsaicin (a dietary polyphenol) was shown to contribute to a reduction in weight gain, in mice fed with HFD, and excess of the Akkermansia spp., Bacteroides spp., and Coprococcus spp. [212]. Another work reveals that cranberry extract exerts positive metabolic effects by enhancing the dietary features caused by high fat/high sucrose (HFHS), which is linked to a proportional growth in Akkermansia spp. population [198].
The dietary replacement of an HFD with grape polyphenols contributed to dramatical improvements in the composition of the intestinal microbial population, which included a decline in the Firmicutes-to-Bacteroidetes ratio and an improvement in A. muciniphila. These modifications will provide some protection against the harmful effects of an HFD [200].

Pre-/Probiotic Formulations Using Nanotechnology: Applications in MetS
Emerging uses of nanotechnology [213] have recently been developed and applied in prebiotics and probiotics [214,215]. Probiotic nanotechnology is an evolving discipline that creates and allows entirely new options for the use of probiotics. In contrast with their use in medicines and pharmaceuticals, their uses in agriculture and the food industry are comparatively new. Currently, the production of nano-encapsulated probiotics is the fundamental application of probiotic nanotechnology. In order to improve flavor, texture, and consistency, the nanostructured food ingredients are created. Nanotechnology applications in producing organic food need vigilance, since their environmental and human health effects are not well established. There are currently no laws directly regulating or restricting the production of nanosized particles, primarily because of a lack of awareness of hazards [216].

Prebiotics Formulations
In the last few years, various forms of prebiotic formulations have gained further interest. The most favorable and most studied formulation of these prebiotics is the encapsulation into emulsions or structures dependent on nano and microparticles.
Maria et al. have therefore investigated the effect of the incorporation of numerous prebiotic fibers on the rheological and technological properties and the microstructure of an emulgated meat product (bologna). It was concluded that the stability of the released meat is improved with the inclusion of partial manhole starch and prebiotic fibers, to ensure that the provision of bologna sauce is safe [217].
Kazmierczak et al. identified a revolutionary strategy, loading these nanoparticles onto a biological carrier that delivers the treatments to a target site (a novel, nontoxic, therapeutic strain of Salmonella typhimurium that has been engineered as a biological delivery system hindering prostate cancer cells). Hu et al. also presented an example of nanoparticle-embodied probiotic bacteria that demonstrated an effective strategy to produce powerful and scalable DNA vaccines by covering live bacterial cells with synthetic nanoparticles [218,219]. Feher documented the use of nano-sized particles for prevention and treatment of neuroinflammation [220]. In reality, as an alternative to classical antibiotics to tackle bacterial resistance, probiotics attract the particular interest of people. Kim et al. recommended that prebiotics be produced to improve the function of probiotics by conjugating pathalic anhydride with dextran to create prebiotic formulations composed of Pediococcus acidilactidi [221]. They measured the cellular effects and the antimicrobial properties of the manufactured nanomaterial. Increased development of antimicrobial peptides using a self-defensing mechanism was observed for P. acidilactidi in phthalyl dextran nanoparticles, with an increased antimicrobial impact against both type of microorganisms-Gram (+) and Gram (−). Prebiotic phthalyl inulin nanoparticles were previously documented to boost P. acidilactici's antimicrobial activities as well [222]. The increased antimicrobial activity of L. plantarum-treated phthalyl pullulan nanoparticles with Escherichia coli K99 and Listeria monocytogenes was also documented by Hong [223].

Probiotics Formulations
The nanoparticle was internalized by an energy-related and galactose-dependent conveyor mechanism in L. plantarum and higher amounts of plantaricin were secreted out from the nanoprobiotic produced as compared to the probiotic alone. The application of probiotic spores as a supply chain for chemotherapeutic drugs was recently proposed. In the autonomous production of nanoparticles in the gastrointestinal tract, Song et al. developed modified deoxycholic acid spores packed with doxorubicin and sorafenib [224]. Such a strategy targets the safety of medications in order to boost bioavailability in oral administration. In addition, disintegrated hydrophobic protein and hydrophilic deoxycholic acid are the source of the release, which increases the basolateral release of drugs by enhancing epithelial cells through the pathway to bile acid. Aziz identified the anticarcinogenic activity of nanoparticles of silver/Lactobacillus rhamnosus GG [225]. With the MTT test, the authors demonstrated that the viability of the HT-29 cell lines was substantially diminished, and apoptosis was caused by the addition of the maximum measured concentration of nanoparticles. It was observed that the synthesis of silver/Lactobacillus rhamnosus GG nanoparticles are cost efficient and offers a feasible biomedical nano-probiotic approach.
Fung's work should be remembered, where the use of nanofibers to nanoencapsulate L. acidophilus, using 8 percent polyvinyl alcohol as a probiotic encapsulant for electrospinning technology, was suggested after investigating agro-waste-based nanofibers [226].
In Ebrahimnejad's recent paper, chitosan is used as a probiotic bacteria to enhance the viability and survival of L. acidophilus nanoencapsulation towards gastrointestinal environments. Their usage as a probiotic bacterium was identified by the nanoencapsulation of probiotic bacteria [227].

Symbiotic Formulations
In a study of the impact of including inulin in alginate beads, Atia et al. developed an embedded oral-symbiotic supplement and observed its capacity to protect three different probiotic strains: P. acidilactici, L. reuteri, and L. salivarius. The bacteria were not found to be impaired by the encapsulation of the antimicrobial and probiotic properties [215].
A symbiotic nano-emulsion based on inulin was developed by Krithika and Preetha for enhancing probiotic stability; as a transport system for various probiotics in food products, the whey protein concentrate/inulin nano complex may be proposed [228]. Salmeron et al. published their work on the production of the fermented beverages with synbiotic properties in the field of food produced to boost human health and well-being by integrating specific and special bioactive nanoparticles, to enhance organoleptic proprieties, nutrient absorption, and the supply of bioactive nutrients and compounds [229].

Concluding Remarks and Future Perspectives
The administration of prebiotics and/or probiotics in MS is accompanied by numerous benefits in terms of improving metabolic parameters such as BMI, abdominal circumference, basal or postprandial glycemia, insulin resistance, and inflammation parameters. The quality of the microbial flora also influences numerous pathophysiological processes specific to MetS and T2DM (i.e., insulin resistance, pro-inflammatory status, regulation of blood sugar or appetite, etc.). In recent years, the attention paid to the bacterium A. muciniphilia is due to the observed and proven benefits in reducing body weight, with numerous studies (as highlighted in this review) supporting the need for colonization with Akkermansia spp. Moreover, the consumption of foods rich in polyphenols positively influences the bacterial microflora. Nanotechnology can also offer numerous solutions for overcoming the gastric barrier, as well as for achieving adequate concentrations of compounds at the intestinal level, the administration of pre-/probiotics being one of the applications of this expanding field.
Overall, several studies have shown that favorable manipulation of the probiostatic or prebiotic usage of the intestinal microbiota and the immune system will boost typical parameters of MetS. However, more properly planned animal and human experiments may reveal new information in order to justify the controversies surrounding the impact and doses of pre-/probiotics on the MetS risk factors and offer a more complete explanation of the function of the acts concerned. Specific clarified considerations include the minimum required for producing beneficial benefits, the duration of supplementation, persistence of this influence, and potential contraindications. Diverse hypotheses were newly introduced to clarify the advantages of probiosis to methyl microorganisms; however, there were substantial issues that must be considered with respect to probiotic effects and their action mechanisms regarding the strain specificities and duration of administration. Furthermore, the chemical composition of prebiotic compounds is a crucial consideration for the regulation of microorganisms of the intestinal microbiota. To explain the efficacy of pre-/probiotics on MetS prevention and management and endorse their future application of clinical procedures, scientifically randomized, placebo-controlled trials, utilizing, for example, multiple subjects, should be undertaken [230].
Pharmacists are encouraged to be familiar with pre-/probiotic products that are generally available so that they can make reasonable decisions for particular groups of patients. It is important for these drug specialists to realize when a probiotic should be prescribed, that not all strains are the same, and that certain benefits of this type of supplement are unique to each type of condition or disorder. Equipped with proper expertise, pharmacists may advise patients related to the strains or mixtures, in close correlation with the action/therapeutic advantage that the subject is looking for.
Of course, there are some additional features of a quality probiotic product, as well as a scientifically sustained benefit that must be mentioned. The following data should always be included on the probiotic label: adequate storage directions; CFU amount before date of expiry; date of expiry; details on the producer company's contact; microbe names/genus/species/strains; and the prescribed dosage or section in compliance with clinical evidence, etc. When a probiotic is recommended, it must be well-known that not every strain is the same, that the benefits are strain-specific, so the patient must pick, related to the advantage, a scientifically proven strain. Moreover, they must select a probiotic according to scientific data, at the dosage given for that strain (or mixture of strains), and be careful when considering the following specific aspects: a high CFU count (more not being always good) and probiotic formulations of multi strains (many of them may not having clinical evidence).