Next Article in Journal
The Effect of Visible Light on Cell Envelope Subproteome during Vibrio harveyi Survival at 20 °C in Seawater
Next Article in Special Issue
A Yarrowia lipolytica Strain Engineered for Pyomelanin Production
Previous Article in Journal
Effective Small Molecule Antibacterials from a Novel Anti-Protein Secretion Screen
Previous Article in Special Issue
Increasing the Production of Volatile Fatty Acids from Corn Stover Using Bioaugmentation of a Mixed Rumen Culture with Homoacetogenic Bacteria
Article

Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation

1
Graduate School of Education, Mie University, 1577 Kurimamachiya-cho Tsu, Mie 514-8507, Japan
2
Faculty of Education, Mie University, 1577 Kurimamachiya-cho Tsu, Mie 514-8507, Japan
3
Graduate School of Bioresources, Mie University, 1577 Kurimamachiya-cho Tsu, Mie 514-8507, Japan
*
Author to whom correspondence should be addressed.
Academic Editor: Birgitte K. Ahring
Microorganisms 2021, 9(3), 593; https://doi.org/10.3390/microorganisms9030593
Received: 7 February 2021 / Revised: 6 March 2021 / Accepted: 11 March 2021 / Published: 13 March 2021
(This article belongs to the Special Issue Microbes for Production of Biofuels and Bio-Products)
The cultivation of the cellulolytic bacterium, Clostridium thermocellum, can have cost-effective cellulosic biomass utilizations, such as consolidated bioprocessing, simultaneous biological enzyme production and saccharification. However, these processes require a longer cultivation term of approximately 1 week. We demonstrate that constituents of the C. thermocellum membrane vesicle fraction significantly promoted the growth rate of C. thermocellum. Similarly, cell-free Bacillus subtilis broth was able to increase C. thermocellum growth rate, while several B. subtilis single-gene deletion mutants, e.g., yxeJ, yxeH, ahpC, yxdK, iolF, decreased the growth stimulation ability. Metabolome analysis revealed signal compounds for cell–cell communication in the C. thermocellum membrane vesicle fraction (ethyl 2-decenoate, ethyl 4-decenoate, and 2-dodecenoic acid) and B. subtilis broth (nicotinamide, indole-3-carboxaldehyde, urocanic acid, nopaline, and 6-paradol). These findings suggest that the constituents in membrane vesicles from C. thermocellum and B. subtilis could promote C. thermocellum growth, leading to improved efficiency of cellulosic biomass utilization. View Full-Text
Keywords: cellulosic biomass utilization; membrane vesicle; cell–cell communication; Clostridium thermocellum; Bacillus subtilis; metabolome analysis cellulosic biomass utilization; membrane vesicle; cell–cell communication; Clostridium thermocellum; Bacillus subtilis; metabolome analysis
Show Figures

Figure 1

MDPI and ACS Style

Ichikawa, S.; Tsuge, Y.; Karita, S. Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation. Microorganisms 2021, 9, 593. https://doi.org/10.3390/microorganisms9030593

AMA Style

Ichikawa S, Tsuge Y, Karita S. Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation. Microorganisms. 2021; 9(3):593. https://doi.org/10.3390/microorganisms9030593

Chicago/Turabian Style

Ichikawa, Shunsuke, Yoichiro Tsuge, and Shuichi Karita. 2021. "Metabolome Analysis of Constituents in Membrane Vesicles for Clostridium thermocellum Growth Stimulation" Microorganisms 9, no. 3: 593. https://doi.org/10.3390/microorganisms9030593

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop