Thiosemicarbazone Copper Chelator BLT-1 Blocks Apicomplexan Parasite Replication by Selective Inhibition of Scavenger Receptor B Type 1 (SR-BI)

Coccidian parasites are obligate intracellular pathogens that affect humans and animals. Apicomplexans are defective in de novo synthesis of cholesterol, which is required for membrane biosynthesis and offspring formation. In consequence, cholesterol has to be scavenged from host cells. It is mainly taken up from extracellular sources via LDL particles; however, little is known on the role of HDL and its receptor SR-BI in this process. Here, we studied effects of the SR-BI-specific blocker BLT-1 on the development of different fast (Toxoplasma gondii, Neospora caninum, Besnoitia besnoiti) and slow (Eimeria bovis and Eimeria arloingi) replicating coccidian species. Overall, development of all these parasites was significantly inhibited by BLT-1 treatment indicating a common SR-BI-related key mechanism in the replication process. However, SR-BI gene transcription was not affected by T. gondii, N. caninum and B. besnoiti infections. Interestingly, BLT-1 treatment of infective stages reduced invasive capacities of all fast replicating parasites paralleled by a sustained increase in cytoplasmic Ca++ levels. Moreover, BLT1-mediated blockage of SR-BI led to enhanced host cell lipid droplet abundance and neutral lipid content, thereby confirming the importance of this receptor in general lipid metabolism. Finally, the current data suggest a conserved role of SR-BI for successful coccidian infections.


Introduction
Coccidia comprise a large group of protozoan parasites belonging to the apicomplexan phylum. In general, coccidian parasites are distributed in two families, Sarcocystidae and Eimeriidae. Sarcocystidae parasites have a heteroxenic life cycle, while the majority of Eimeriidae species present with a monoxenic life cycle [1]. Despite several conserved features among these families, coccidian parasites also show a tremendous divergence in host range, host cell specificity and clinical outcomes. In Sarcocystidae, the clinical scenario is largely a consequence of an extraintestinal merogonic (asexual) replication. In particular, Toxoplasma gondii, which is a widely distributed zoonotic parasite with a broad range of suitable intermediate hosts, commonly induces abortions in humans and sheep [2,3]. In contrast, the closely related coccidian Neospora caninum is not zoonotic, but it is considered to be a major abortive agent in the bovine industry [4,5]. Additionally, Besnoitia besnoiti is the causal agent of bovine besnoitiosis, a re-emerging disease in Europe, which leads to massive alterations of skin and mucosa in cattle and to infertility in bulls [6,7]. Interestingly, Eimeriidae parasites are species-specific pathogens, most of them with a marked tropism towards intestinal tissues. Two of the most pathogenic species in ruminants, Eimeria bovis (cattle) and Eimeria arloingi (goats), develop macromeronts in highly immunoreactive endothelial host cells during their first merogony and can provoke life-threading diarrhoea in young calves and goat kids, respectively, thereby generating an enormous economic impact on bovine and caprine industries worldwide [8].
During early host infection, coccidian parasites proliferate asexually within suitable nucleated host cells. Nevertheless, major differences exist within coccidian families regarding cell tropism and kinetics of development, largely driven by parasite stage and species [1]. Sarcocystidae parasites rapidly proliferate within a rather wide range of host cell types releasing their progeny (tachyzoites) a few days or even hours after infection [9,10]. In contrast, some pathogenic ruminant Eimeria species perform the long-lasting first merogony within distinct host cells, such as endothelial cells in the lacteal of the intestinal villi [11], releasing a high number of merozoites I (>120.0000) after 15-18 days in vitro [12,13]. In this context, primary endothelial cells were proven to be appropriate for the development of several coccidian species. This in vitro system, which is closely related to the in vivo scenario, allows intracellular replication [12][13][14][15][16] and consequently delivers a methodological bridge for analysing these divergent families in the same host cell type, thereby avoiding host cell type-driven variations.
During merogonic replication, considerable amounts of nutrients are required to support high proliferation rates. However, apicomplexans miss some pivotal metabolic pathways, which may be suitable targets for novel therapeutic strategies. In particular, apicomplexan species rely on host cells to fulfil their cholesterol requirements [17]. From a physiological perspective, cells mainly acquire cholesterol from circulating lowdensity lipoproteins (LDL) with the LDLR (LDL receptor)-related endocytic pathway representing the best-characterized cholesterol uptake route [18,19]. Exogenous LDL supply proved essential for several fast replicating coccidia, such as T. gondii, N. caninum and B. besnoiti [20][21][22]. Referring to this mechanism, cholesterol is internalized by clathrinmediated endocytosis of the LDL-LDLR complex and delivered to lysosomes where cholesteryl esters are cleaved by acid lipase, thereby releasing free cholesterol for cellular needs [23,24]. The mechanism of cholesterol exit from late endosomes is still under debate; nevertheless, the involvement of Niemann-Pick type C protein 1/2 (NPC1-2) is largely accepted [23]. This protein promotes transfer of cholesterol into other membranes (i.e., cytoplasmic, endosomal, mitochondrial) or its incorporation into the endoplasmic reticulum for formation of lipid droplets (LDs), which indeed are the key cholesterol sources for intracellular pathogens [25].
As excess accumulation of free cholesterol is toxic, it should be effluxed from the cell. This process requires high-density lipoprotein (HDL) particles functioning as extracellular acceptors and is driven by transporters from the ATP-binding cassette (ABC) family, such as ABCA1 [18,19,26]. Additionally, cholesterol efflux in other cell types, such as endotheliocytes and macrophages, is modulated by the HDL receptor, scavenger receptor B I (SR-BI) [27][28][29]. This transmembrane protein mediates a gradient-dependent bidirectional flux of cholesteryl esters from HDL and other lipoproteins via a unique non-endocytic route [30,31]. Thus, SR-BI plays a dual role in reverse cholesterol transport, promoting not only cholesterol efflux from peripheral cells, but also its uptake by hepatocytes for biliary disposal [28,29]. In this context, SR-BI has a marked tissue-specific expression, which is enhanced especially in steroidogenic tissue and hepatocytes. Nevertheless, its considerable expression in neoplastic cells, such as prostate and breast cancer cells additionally indicates its tight association with high proliferative activities and malignant cell phenotypes [32]. Of note, SR-BI is also capable of incorporating cholesteryl esters from LDL particles via a non-endocytic route [31], representing an alternative route for LDL-related cholesterol acquisition. Interestingly, the apicomplexan parasite Plasmodium spp. significantly relies on SR-BI interactions for hepatocyte invasion and intracellular proliferation during hepatic stage of its life cycle [33,34]. However, for other apicomplexan parasites, no SR-BI-related data are available so far.
The aim of this work was to evaluate the role of SR-BI in coccidian host cell invasion and obligate intracellular replication. To additionally address potentially conserved SR-BIrelated mechanisms, comparative inhibitor studies were performed on several protozoan parasites covering both fast (T. gondii, N. caninum, B. besnoiti) and slow (E. bovis and E. arloingi) replicating coccidian species. Overall, here, we confirmed that SR-BI indeed seems to be involved in successful replication of all these parasites when replicating in bovine primary endothelial cells.

BLT-1 Treatments of Host Cells and Parasite Infections
For infection experiments on fast replicating coccidia (T. gondii, N. caninum and B. besnoiti), BUVEC (n = 5) were seeded in 12-well plates (Sarstedt, Nümbrecht, Germany) pre-coated with fibronectin (1:400; Sigma-Aldrich). A BLT-1 (Sigma-Aldrich) stock solution was prepared in dimethyl sulfoxide (DMSO; Sigma-Aldrich, 10 mM, stored at −20 • C), diluted in modECGM and administered at different concentrations (0.25-2 µM) to fully confluent cell monolayers 48 h before infection. Plain modECGM with DMSO (0.02%) served as vehicle control. Following pre-treatments, the inhibitor-supplemented medium was removed, and host cells were infected with tachyzoites at a multiplicity of infection (MOI) of 5:1 for 4 h under inhibitor-free conditions. Then, extracellular tachyzoites were removed and a fresh BLT-1-supplemented medium was re-administered. For infection rate estimation, phase-contrast images were acquired at 24 h p.i. with an inverted microscope (IX81, Olympus, Shinjuku City, Tokyo, Japan) equipped with a digital camera (XM10, Olympus). For the evaluation of inhibitory efficacy, tachyzoites present in cell culture supernatants at 48 h p.i. of BLT-1-treated cells and non-treated controls were collected (800× g; 5 min) and counted in a Neubauer chamber.
For analysis of E. bovis or E. arloingi first merogony and merozoite I production, BUVEC were cultured in 12-well plates (Sarstedt). Thereafter, confluent cell layers were infected with either 7.8 × 10 4 E. bovis or E. arloingi sporozoites for 24 h. Every third day, the culture medium was replaced with fresh modECGM. From 10 (E. bovis) or 15 (E. arloingi) days p.i. onwards, infected cell cultures were treated with BLT-1 (2 µM) or vehicle (DMSO 0.02%), with inhibitor-supplemented fresh medium being replaced every 2-3 d. For analyses of E. bovisor E. arloingi first merogony development over time, 50 (E. bovis) or 40 (E. arloingi) macromeronts were analysed per BUVEC isolate (n = 3 and 4, respectively). In case of E. bovis cultures, BLT-1-driven effects on macromeront development were assessed via estimating the number and size of meronts per area at days 15 and 19 p.i. using the CellSens Dimension ® v1.7 software (Olympus, Shinjuku City, Tokyo, Japan). Moreover, E. bovis proliferation was determined via qPCR-based (EbMIC4-qPCR) analyses of merozoite I production at 24 d p.i., as previously described [14,38]. In case of the E. arloingi cultures, macromeront numbers and sizes were studied at days 17, 19, 21, 24 and 26 p.i. From day 21 p.i. onwards, supernatants of infected monolayers were harvested and stored at −80 • C for qPCR-based merozoite I quantification, as previously reported [13].
For LD visualization, BUVEC were seeded into 35 mm tissue culture µ-dishes (Ibidi ® ) and treated with the vehicle and BLT-1 for 48 h as described above. Then, the treated cells were loaded with BODIPY 493/503 (2 µg/mL, 1 h, 37 • C, 5% CO 2 ; Cayman Chemical, Ann Arbor, MI, USA) as described elsewhere [15]. Cellular LDs in the BLT-1-treated cells and non-treated controls (n = 50 per condition) were manually counted based on morphology and BODIPY 493/503-driven signal accumulation in endothelial cells [39] and expressed as the number of LDs per cell.

RT-qPCR for Relative Quantification of SR-BI Gene Transcripts
BUVEC (n = 5) grown in 25 cm 2 culture tissue flasks (Greiner, Frickenhausen, Germany) were infected with T. gondii, N. caninum or B. besnoiti tachyzoites (MOI = 5:1). Infected and non-infected host cells were equally processed for total RNA isolation at four different time points after infection (3, 6, 12, 24 h p.i.). For total RNA isolation, the RNeasy kit (Qiagen, Germantown, MD, USA) was used according to the manufacturer's instructions. Total RNAs were stored at −80 • C until further use. In order to remove any traces of genomic DNA, a DNA digestion step was performed. Therefore, 1 µg of total RNA was treated with 10 U DNase I (Thermo Scientific, Waltham, MA, USA) in 1× DNase reaction buffer (37 • C, 30 min). Thereafter, DNase was inactivated by heating the samples (65 • C, 10 min). The efficiency of genomic DNA digestion was confirmed by no-RT-controls in each RT-qPCR experiment, while cDNA synthesis was performed using SuperScript IV (Invitrogen TM , Waltham, MA, USA) according to the manufacturer's instructions. Briefly, for first-strand cDNA synthesis, 1 µg of DNase-treated total RNA was added to 0.5 µL of 50 µM oligo(dt), 1 µL of 50 ng/µL random hexamer primer, 1 µL of 10 mM dNTP mix in a total volume of 10 µL. Thereafter, the samples were incubated at 65 • C for 5 min and then immediately cooled on ice. Then, 4 µL of 5× SSIV buffer, 1 µL 0.1 M DTT, 1 µL RNAse-free H 2 O and 0.5 µL SuperScript IV enzyme were added, obtaining a total volume of 16.5 µL. The samples were incubated at 23 • C for 10 min followed by 50 • C for 10 min and an 80 • C inactivation step for 10 min.
The probes were labelled at the 5 -end with reporter dye FAM (6-carboxyfluorescein) and at the 3 -end with quencher dye TAMRA (6-carboxytetramethyl-rhodamine). The SR-BI primer and the probe sequence were designed as follows: Bos taurus SR-BI forward 5 -CCACCTCATCAATCAGTAC-3 ; reverse 5 -TCGGAATGCCAATAGTTG-3 and probe ACTCCATTCCACTTGTCCACGA; qPCR amplification was performed on a Rotor-Gene Q Thermocycler (Qiagen) in duplicates in 10 µL total volume containing 400 nM forward and reverse primers, 200 nM probe, 10 ng cDNA and 5 µL 2× PerfeCTa qPCR FastMix (Quanta Biosciences, Gaithersburg, MD, USA). The reaction conditions were as follows: 95 • C for 10 min, 40 cycles at 95 • C for 10 s, 60 • C for 15 s and 72 • C for 30 s. No-template controls and no-RT reactions were included in each experiment. As the reference housekeeping gene, GAPDH was used as previously reported [14,35].

Cell Viability Assessment
Cell toxicity of BLT-1 was evaluated by colorimetric XTT tests (Promega, Madison, WI, USA) according to the manufacturer's instructions. Briefly, BUVEC (n = 3) were cultured in 96-well plates (Greiner) and treated with BLT-1 (2 µM) in a total volume of 50 µL for 72 h. Thereafter, 50 µL of the XTT working solution were added and the samples were incubated for 4 h (37 • C, 5% CO 2 atmosphere). The resulting formazan products were estimated via optical density (OD) measurements at 590 nm and reference filter 620 nm wavelength using a Varioskan ® Flash Multimode Reader (Thermo Scientific). BUVEC treated with a solvent (DMSO; 0.02%) were used as the negative control.

Statistical Analysis
For statistical analyses, the GraphPad ® Prism 8 (version 8.4.3) software was used. Calculation of Ca ++ fluxes over time was performed by the analysis of the area under the curve (AUC), using the first 90 s before stimulation as the base line and estimating a total duration of 1100 s. Data description was carried out by presenting the arithmetic mean ± standard deviation. In addition, the nonparametric statistical Mann-Whitney test for the comparison of two experimental conditions was applied. In cases of three or more conditions, the Kruskal-Wallis test was used. Whenever global comparison using the Kruskal-Wallis test indicated significance, post hoc multiple comparison tests were carried out by means of Dunn's tests to compare the test conditions with the control ones. Outcomes of the statistical tests were considered to indicate significant differences when p ≤ 0.05 (significance level).

BLT-1 Treatments Induce Dose-Dependent Blockage of Tachyzoite Replication
Before conducting any further experimentation, potential cytotoxic effects of BLT-1 on BUVEC or tachyzoites were monitored via cytotoxicity assays. As depicted in Supplementary Figure S1A, treatments with BLT-1 (2 µM) did not induce significant changes in formazan product formation compared to the vehicle controls (DMSO, 0.02%). Similarly, trypan blue exclusion tests showed a similar average viability for T. gondii, N. caninum and B. besnoiti tachyzoites either treated with 0.02% DMSO (vehicle control) or BLT-1 (2 µM) (Supplementary Figure S1B).
The impact of BLT-1 treatments on the replication of fast proliferating coccidian species (T. gondii, N. caninum and B. besnoiti) was determined by functional assays via counting tachyzoites being released into the medium after 48 h p.i. In principle, BTL-1 treatments induced dose-dependent inhibition of tachyzoite proliferation in all three coccidian species ( Figure 1); however, a difference in single parasite sensitivities was apparent since the effects of different inhibitor doses varied in their magnitude in a species-specific manner. In particular, BLT-1 treatments reduced T. gondii replication by 90.80 ± 2.00% (p < 0.01) and 97.99 ± 1.25% (p < 0.001) at 1 and 2 µM, respectively, without any significant effect at lower concentrations ( Figure 1A). In the case of N. caninum ( Figure 1B) and B. besnoiti ( Figure 1C), 2 µM BLT-1 reduced tachyzoite replication by 64.59 ± 7.76% (p < 0.001) and 47.24 ± 4.39% (p < 0.01), respectively, with no significant inhibitory effects at lower concentrations. Thus, B. besnoiti appeared as the less BLT-1-sensitive and T. gondii as the most sensitive of these three coccidian species. In addition, live cell 3D holotomography confirmed respective effects of BLT-1 treatments (2 µM) in all three parasites species, illustrating a reduction in meront size in T. gondii- (Figure 1(A1,A2)), N. caninum- (Figure 1(B1,B2)) and B. besnoitiinfected (Figure 1(C1,C2)) BUVEC, which was mainly driven by a reduction in tachyzoite numbers within meronts.

BLT-1 Treatments Interfere with E. bovis and E. arloingi Macromeront Formation and Block Merozoite I Production
The highly pathogenic ruminant Eimeria species E. bovis (cattle) and E. arloingi (goats) develop in host endothelial cells during the first merogony with macromeront formation. The impact of BLT-1 treatment was studied throughout the first merogony of both species by estimating macromeront sizes and numbers in addition to merozoite I production. Overall, a detrimental effect of BLT-1 treatments on macromeront development and merozoite I production was stated in both Eimeria species.
In case of E. bovis, infected host cells were treated from 10 days p.i. onwards, i.e., the treatments started at immature meront stages. Microscopic monitoring revealed dramatic effects of BLT-1 treatments since immature meronts hardly developed any further under treatment (Figure 2A). Overall, blockage of SR-BI via 2 µM BLT-1 treatments induced a reduction in macromeront numbers per area, as observed on days 15 (p = 0.0721; 80% reduction) and 19 (p = 0.0141; 87% reduction) p.i. (Figure 2B). Moreover, those meronts that were still able to form proved to be smaller ( Figure 2C; 15 d p.i.: p = 0.1149; 19 d p.i.: p = 0.0075) than the ones in non-treated control cells. Consequently, merozoite I proliferation was significantly blocked by 95% when compared to the controls ( Figure 2D; p = 0.0286).
E. arloingi-infected BUVEC were treated with BLT-1 from day 15 p.i. onwards, i.e., when macromeronts were still immature, and were thoroughly monitored microscopically ( Figure 3A). Overall, the microscopic effects differed substantially from those observed in E. bovis cultures and appeared to be less prominent. Thus, in terms of macromeront size, parasite development seemed to be similar in the treated and non-treated E. arloingi-infected BUVEC. However, from day 17 p.i. onwards, merozoite I formation started in the control cells but was absent in the treated cells. From day 21 p.i. onwards, mature merozoites I were visible in macromeronts of the non-treated cells, while macromeronts of BLT-1-treated cultures showed degradation, vacuolization, and a lack of merozoites I until the end of the experiment. In contrast to the treated cells, fully developed merozoites I were released into the cell culture supernatant beginning at 24 days p.i. (Figure 3A, 24 d p.i.). In order to control delayed merozoite I formation under BLT-1 treatment, BUVEC cultures were microscopically monitored until day 35 p.i. During this timeframe, no merozoites I were formed, and continuous macromeront degradation was observed. To investigate further the inner structure of macromeronts in the BLT-1-treated cells, 3D holotomographic imaging was performed at day 22 p.i. (Figure 3A, right panel). Here, macromeronts presented equal inhibitor-driven effects of vacuolization in both compartments, the host cell and the macromeront. Furthermore, merozoites I could not be identified since macromeront content appeared as an undistinguishable mass, which is a characteristic feature for degrading Eimeria macromeronts. Interestingly, even if new offspring failed to form, macromeronts continued to grow until day 26 p.i., thereby hampering any significant effects of BLT-1treatments on meront size ( Figure 3B). E. arloingi-infected BUVEC were treated with BLT-1 from day 15 p.i. onwards when macromeronts were still immature, and were thoroughly monitored microsc cally ( Figure 3A). Overall, the microscopic effects differed substantially from those served in E. bovis cultures and appeared to be less prominent. Thus, in terms of ma meront size, parasite development seemed to be similar in the treated and non-treate arloingi-infected BUVEC. However, from day 17 p.i. onwards, merozoite I forma started in the control cells but was absent in the treated cells. From day 21 p.i. onwa

BLT-1 Treatments Alter Neutral Lipid Contents and Cholesterol Distribution in BUVEC
Given that anti-parasitic effects of BLT-1 primarily seemed to be attributed to host cell alterations and considering that all T. gondii, N. caninum and B. besnoiti species have already been reported to depend on host cell lipid disposal [22,25], we next evaluated BLT-1-mediated effects on endothelial cell phenotype by performing RI-based 3D holotomographic live cell imaging. As illustrated in Figure 6, 2 µM BLT-1 treatments did not induce major phenotypic changes of living BUVEC; nevertheless, we found a discrete increase in small dense globular structures within the cytoplasm of treated cells (Figure 6 arrows). These vesicle-like structures showed the average RI of 1.366 ± 0.011 (n = 50) which is consistent with prior reports on LDs [22]. To confirm this finding, we additionally stained BUVEC for cellular distribution of neutral lipids via BODIPY 493/503. BLT-1 treatments induced a change in neutral lipid signal distribution by increasing the cytoplasmic presence of BODIPY 493/503-positive vesicles throughout the cell by 45.1% when compared with vehicle-treated controls (p = 0.004) ( Figure 6A,B). We also confirmed these microscopebased observations in a quantitative manner via FACS analysis on BODIPY 493/503-stained live endothelial cells. As expected, BLT-1 treatments induced a significant increase in the neutral lipid-related mean fluorescence intensity (26.1%, p = 0.0159, Figure 6C).

Discussion
Since apicomplexan parasites are auxotrophic for cholesterol biosynthesis, they need to obtain this molecule from their host cells to ensure successful proliferation [17]. During merogonic replication of coccidian parasites, host cell uptake of exogenous LDL represents the major mechanism of cholesterol acquisition to fulfil parasite metabolic requirements [20][21][22]44]. This route relies on endocytosis of LDL-LDLR, being thereafter LDL-derived cholesterol released for cell requirements in a NPC1-dependent process [18]. Interestingly, alternative non-endocytic mechanisms of cholesterol uptake have also been suggested for endothelial cells [27]. In this context, endotheliocytes might obtain cholesterol from acetylated or oxidized LDL via the acLDL receptor and the lectin-type oxidized LDL receptor 1 (LOX-1, syn. OLR1) [27]. Moreover, participation of scavenger receptor B type I (SR-BI) representing the canonical HDL receptor in an alternative non-endocytic route for cholesteryl ester uptake from plasmatic LDL molecules was proposed [28,31], thereby offering an alternative and LDLR-independent scavenging pathway for endothelial coccidian infections.
To analyse involvement of SR-BI in coccidian parasite proliferation, the SR-BI-specific inhibitor BLT-1 was used [45,46]. This compound has a high inhibitory capacity without affecting LDL-endocytic cholesterol acquisition delivering a suitable tool for SR-BIrelated analyses [45,46]. The current data show that BLT-1 treatments significantly reduced T. gondii-, N. caninumand B. besnoiti tachyzoite replication in primary host endothelial cells, T. gondii being the most affected by this treatment. So far, this is the first report on antiproliferative capacity of BLT-1 treatments in fast proliferating coccidian species. Of note, prior reports showed importance of SR-BI in Huh7 cell infections with the apicomplexan parasite P. berghei via BLT-1 treatments and siRNA assays [33]. Interestingly, these findings were corroborated by reduction of parasite burden in SR-BI-deficient mice, demonstrating participation of SR-BI-mediated pathways in hepatic P. berghei replication in vivo [33], suggesting a common mechanism for apicomplexans. However, apparent differences in host cell type and parasite biology limit valid comparisons with present data. In this context, it should be highlighted that the capacity of SR-BI to incorporate cholesteryl esters and other lipids from HDL particles is largely accepted for the hepatic tissue [27][28][29]. In contrast, its function in endothelial cell cholesterol metabolism currently seems limited to its typical role as HDL receptor [28,29]. It is worth noting that T. gondii, N. caninum and B. besnoiti generate their offspring via endodyogeny, whilst species of the genus Plasmodium, such as P. berghei and P. falciparum, undergo schizogony during their hepatic phase in vivo [47]. In this scenario, to address if current anti-coccidian effects of BLT-1 in endothelial cells represented a conserved phenomenon or rather a consequence of particular characteristics of host cell type or of specific parasite asexual division mechanisms, we additionally evaluated the impact of this inhibitor on E. bovis and E. arloingi merogony I in BUVEC. These eimerian parasites undergo schizogony during their long-lasting first merogony (more than 18 days in vitro), which is restricted to endothelial host cells and which requires considerable amounts of cholesterol to fulfil macromeront formation and massive offspring production, resulting in ≥ 120,000 merozoites I per macromeront [12,14,22,48]. In line with the fast proliferating species, BLT-1 treatment effectively reduced macromeront development in both pathogenic ruminant Eimeria species, suggesting conserved involvement of SR-BI in apicomplexan replication. However, as reported here for fast proliferating coccidian species, we also detected slight species-specific differences in BLT-1 effects on E. bovis and E. arloingi macromeront development. Thus, whilst E. bovis macromeronts hardly developed at all under BLT-1 treatments, E. arloingi macromeronts seemed to be formed but failed to produce merozoites I and were prone to degradation. So far, this is the first report on the participation of cholesterol-related routes in caprine E. arloingi first merogony. In contrast, for E. bovis, its dependence on host cell de novo cholesterol biosynthesis and LDL uptake was already reported [14,49].
Due to their characteristic obligate intracellular life style, coccidian parasites must actively invade host cells [9]. Therefore, here, we evaluated the impact of BLT-1 treatments on free infective stages (tachyzoites in case of T. gondii, N. caninum and B. besnoiti and sporozoites for E. bovis and E. arloingi) and additionally controlled whether host cell pretreatments may impair active parasite invasion. Referring to infective stages, interestingly, we found stage-specific effects of BLT-1 treatments. Thus, tachyzoite treatments resulted in an impairment of T. gondii, N. caninum and B. besnoiti host cell invasion process, thereby either indicating the presence of parasite molecules interacting with BLT-1 in tachyzoites or non-specific side effects of this treatment. In contrast, Eimeria spp. sporozoites were not at all affected by BLT-1 treatments and subsequent infection rates did not differ from those of untreated stages, thereby eventually questioning the hypothesis of non-specific side effects. Referring to host cells, BLT-1 pre-treatments had neither an effect on subsequent parasite invasion nor host cell permissiveness in none of parasites studied here. The latter finding contrasts with observations on Plasmodium spp. infections since SR-BI is considered to be the key surface molecule for host cell recognition during sporozoite invasion [33,34]. Given that no data are available on detailed mechanism of direct anti-invasive BLT-1-triggered effects in tachyzoites, we additionally evaluated effects of treatments on tachyzoite Ca ++ homeostasis. In parallel with impaired invasion, BLT-1 triggered enhanced Ca ++ fluxes in free tachyzoites of T. gondii, N. caninum and B. besnoiti over time. This is of special interest since Ca ++ is well-recognised as a pivotal second messenger for coccidian host cell invasion [9,50], suggesting that BLT-1 treatments could result in untimely Ca ++ mobilization during the invasion process. Possible implications of BLT-1 on Ca ++ homeostasis have not been addressed previously, but BLT-1 treatment has been linked to phenotypical changes in zebrafish development [51], implying possible side effects driven by this compound. Nonetheless, further detailed studies are necessary to establish the impact of BLT-1 on coccidian Ca ++ homeostasis.
From a mechanistic perspective, exogenous cholesterol is mainly incorporated by LDL via the canonical LDL-LDLR endocytic route [18,24]. Considering possible involvement of SR-BI in BUVEC-mediated cholesterol acquisition, we functionally evaluated the impact of BLT-1 treatments on cellular neutral lipid contents. Overall, microscopic analyses showed that BLT-1 treatment incremented the number of neutral lipid (marker = BODIPY 493/503)-positive vesicles, and FACS analyses confirmed this effect in a quantitative manner. In agreement, live cell 3D holotomographic analysis suggested these globular structures as LDs based on their characteristic RI-values. These findings are in line with the function of SR-BI in endothelial cell cholesterol efflux [27], being a consequence of SR-BI blockage, thereby impairing its capacity for HDL-dependent cholesterol efflux and finally resulting in cellular neutral lipid accumulation [52]. Interestingly, we recently reported that verapamil treatments also reduced T. gondii-, N. caninumand B. besnoiti tachyzoite replication, which was accompanied by enhanced neutral lipid accumulation [15]. It seems, therefore, plausible to speculate that cholesterol efflux impairment may generally affect obligate intracellular coccidian development. However, BLT-1-mediated anti-coccidian effects may also be a consequence of inhibitor-driven impairment of SR-BI HDL-dependent signaling pathways, e.g., via PI3K or ERK1/2 [32].
At the transcriptomic level, steroidogenic and hepatic tissues present higher levels of SR-BI mRNAs [32]. In mammalian endothelial cells, SR-BI gene transcription has been linked to liver X receptor (LXR) activation and 17β-estradiol exposure [53,54]. In this context, the capacity of coccidian parasites to modulate host cell gene transcription and their phenotype to sustain metabolic requirements of parasite replication has already been reported [22,[41][42][43]. Considering this, we evaluated whether T. gondii, N. caninum and B. besnoiti infections modulate SR-BI gene transcription in BUVEC over time. Unexpectedly, no infection-mediated changes in SR-BI gene transcription were detected over time, suggesting that constitutive receptor expression is sufficient to mediate current BLT-1-driven effects.
In summary, here, we reported for the first time anti-proliferative effects of BLT-1 on several coccidian species of medical and veterinary importance. These data suggest SR-BI participation in asexual replication of T. gondii, N. caninum, B. besnoiti, E. bovis and E. arloingi, thereby implying conserved mechanisms in these species. Given that BLT-1 treatments additionally drive Ca ++ fluxes and impair infectivity in free tachyzoites, a non-specific side effect of this treatment may eventually occur in this parasitic stage. Finally, BLT-1-induced neutral lipid accumulation demonstrates that SR-BI is involved in neutral lipid metabolism and efflux in primary bovine endothelial cells.