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Open AccessArticle

High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population

1
Department of Environmental and Preventive Medicine, Faculty of Medicine, Oita University, Yufu City, Oita 879-5593, Japan
2
Department of Gastroenterology, Mongolian National University of Medical Sciences, Ulaanbaatar 14210, Mongolia
3
Endoscopy Unit, Mongolia-Japan Teaching Hospital, Mongolian National University of Medical Sciences, Ulaanbaatar 250573, Mongolia
4
Department of Medicine, Gastroenterology and Hepatology section, Baylor College of Medicine, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(7), 1062; https://doi.org/10.3390/microorganisms8071062
Received: 5 June 2020 / Revised: 25 June 2020 / Accepted: 30 June 2020 / Published: 16 July 2020
(This article belongs to the Special Issue Antibiotic Resistance of Helicobacter pylori)
Mongolia has a high prevalence of Helicobacter pylori infection and the second highest incidence of gastric cancer worldwide. Thus, investigating the prevalence of antibiotic resistance and its underlying genetic mechanism is necessary. We isolated 361 H. pylori strains throughout Mongolia. Agar dilution assays were used to determine the minimum inhibitory concentrations of five antibiotics; amoxicillin, clarithromycin, metronidazole, levofloxacin, and minocycline. The genetic determinants of antibiotic resistance were identified with next-generation sequencing (NGS) and the CLC Genomics Workbench. The resistance to metronidazole, levofloxacin, clarithromycin, amoxicillin, and minocycline was 78.7%, 41.3%, 29.9%, 11.9% and 0.28%, respectively. Multidrug resistance was identified in 51.3% of the isolates investigated which were further delineated into 9 antimicrobial resistance profiles. A number of known antibiotic resistance mutations were identified including rdxA, frxA (missense, frameshift), gyrA (N87K, A88P, D91G/N/Y), 23S rRNA (A2143G), pbp1A (N562Y), and 16S rRNA (A928C). Furthermore, we detected previously unreported mutations in pbp1A (L610*) and the 23S rRNA gene (A1410G, C1707T, A2167G, C2248T, and C2922T). The degree of antibiotic resistance was high, indicating the insufficiency of standard triple therapy in Mongolia. View Full-Text
Keywords: Helicobacter pylori; antibiotic resistance; whole genome sequencing; resistance mutation; next-generation sequencing; Mongolia Helicobacter pylori; antibiotic resistance; whole genome sequencing; resistance mutation; next-generation sequencing; Mongolia
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MDPI and ACS Style

Azzaya, D.; Gantuya, B.; Oyuntsetseg, K.; Davaadorj, D.; Matsumoto, T.; Akada, J.; Yamaoka, Y. High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population. Microorganisms 2020, 8, 1062. https://doi.org/10.3390/microorganisms8071062

AMA Style

Azzaya D, Gantuya B, Oyuntsetseg K, Davaadorj D, Matsumoto T, Akada J, Yamaoka Y. High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population. Microorganisms. 2020; 8(7):1062. https://doi.org/10.3390/microorganisms8071062

Chicago/Turabian Style

Azzaya, Dashdorj; Gantuya, Boldbaatar; Oyuntsetseg, Khasag; Davaadorj, Duger; Matsumoto, Takashi; Akada, Junko; Yamaoka, Yoshio. 2020. "High Antibiotic Resistance of Helicobacter pylori and Its Associated Novel Gene Mutations among the Mongolian Population" Microorganisms 8, no. 7: 1062. https://doi.org/10.3390/microorganisms8071062

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