Salmonella Virulence and Immune Escape

Salmonella genus represents the most common foodborne pathogens causing morbidity, mortality, and burden of disease in all regions of the world. The introduction of antimicrobial agents and Salmonella-specific phages has been considered as an effective intervention strategy to reduce Salmonella contamination. However, data from the United States, European countries, and low- and middle-income countries indicate that Salmonella cases are still a commonly encountered cause of bacterial foodborne diseases globally. The control programs have not been successful and even led to the emergence of some multidrug-resistant Salmonella strains. It is known that the host immune system is able to effectively prevent microbial invasion and eliminate microorganisms. However, Salmonella has evolved mechanisms of resisting host physical barriers and inhibiting subsequent activation of immune response through their virulence factors. There has been a high interest in understanding how Salmonella interacts with the host. Therefore, in the present review, we characterize the functions of Salmonella virulence genes and particularly focus on the mechanisms of immune escape in light of evidence from the emerging mainstream literature.


Introduction
Salmonella is a flagellated rod-shaped Gram-negative facultative anaerobe which infects multiple animal hosts including humans by contaminating a wide variety of foods [1][2][3][4]. Salmonella enterica (S. enterica) is regarded as the most pathogenic species and includes > 2600 serovars characterized until now [5]. With regard to human diseases, Salmonella is divided into two groups: Typhoidal serotypes and thousands of non-typhoidal Salmonella serotypes (NTS). Typhoidal serovars causing typhoid fever include Salmonella enterica serovar Typhi (S. Typhi), Paratyphi (S. Paratyphi), and Sendai (S. Sendai) [6,7]. The most common NTS are serovars Typhimurium (S. Typhimurium), Enteritidis (S. Enteritidis), and Dublin (S. Dublin) [8]. Infection with NTS ordinarily results in gastroenteritis, diarrhea, and fever (almost always present), with a low case fatality [9,10]. In addition to diarrheal disease, non-typhoidal Salmonella infections can invade normally sterile sites, resulting in bacteremia, meningitis, and other focal infections [11,12]. The invasive non-typhoidal Salmonella (iNTS) disease is usually characterized by the presence of the nonspecific fever similar to malaria and other febrile illnesses, resulting in clinically indistinguishable and higher case fatality than the non-invasive infections [11,13]. Different

The Virulence-Related Genes of SPI
The specific regions encoding the virulence-related genes distributed in a cluster of Salmonella chromosomes and plasmids are called SPI. To date, 23 SPIs have been identified and characterized [33,67,68]. The five of these, i.e., SPIs-1-5 are common to all serotypes of Salmonella [69][70][71][72][73][74], whereas SPIs-19-23 are absent in both S. Typhi and S. Typhimurium, and are only present in a few S. enterica serovars including Dublin, Gallinarum, and Derby [67,68], and hence will not be discussed here. From SPIs-1-18, only SPI-1, 4, 9, 14, and 18 encoded effectors play an important role in Salmonella invasion into macrophages and epithelial cells. The virulence effectors secreted by SPI-2, 3, 5-8, 10-13, and 16 are implicated in helping Salmonella withstand the acidic environment, accomplishing the intracellular replication, and immune escape from the host. SPI-1 and SPI-2 contain a large number of virulence genes associated with the intracellular pathogenesis and co-encode T3SS, a molecular syringe which transfers the effectors from the bacteria into the host cell cytoplasm, and in turn, the effector manipulates allowing for bacterial invasion and replication in the host cells [75][76][77][78]. To date, over 40 SPI-1 and SPI-2 effectors have been identified in S. Typhimurium, S. Typhi, and S. Paratyphi A (Table 1) [17]. All serovars seem to have a set of "core" effectors, suggesting that they are critical for virulence in different hosts (PipA, PipB, PipB2, SifA, SipA, SipB, SipC, SipD, SopB, SopD, SpiC, SptP, SseF, SseG, SseL, SteA, and SteD). These effectors play diverse roles during infection. In Table 1, we have summarized the major effectors encoded by SPI-1 and SPI-2 in S. Typhimurium, S. Typhi, and S. Paratyphi A, and their functions. Among these 41 effectors identified in S. Typhimurium, 16 are absent in S. Typhi and S. Paratyphi A (AvrA, GogA, GogB, GtgA, GtgE, SlrP, SopA, SopD2, SpvB, SpvC, SpvD, SrfJ, SsaJ, SseJ, SseK2 and SsrA) [17]. Most of these effectors are from SPI-2, indicating that the role of SPI-2 in typhoidal serovars deserves further investigation. This may be related to the broad host range lifestyle of NTS and reflect the host restriction of TS. SopE2, SifB, SsaV, SseB, and SspH2 have a special mention, as they are present in S. Typhi but absent in S. Paratyphi A (Table 1) [17]. These dissimilarities in typhoidal strains may reflect differences between human restricted lifestyle of S. Typhi and S. Paratyphi. SspH2 promotes the colonization of Salmonella in host cells [79]. SopE2 is shown to contribute to generation of a replicative endosomal compartment in enterocytes and facilitate enterocytes invasion in vivo [80]. SpvB interferes with host intracellular iron metabolism via downregulation of nuclear factor erythroid-derived 2-related factor 2 (NRF2) [81]. SifB, a member of Salmonella-induced filaments (SIFs), is necessary for SIF formation and maintaining the integrity of Salmonella-containing vacuoles (SCVs) [82]. However, the potential function of SifB is still unknown [83]. The details regarding effectors are shown in Table 1 and Figure 1.  SPI-1 is a DNA fragment of around 40 kb with stable genetic traits, and present in all Salmonella. SPI-1 contains the inv, hil, org, spt, spa, sip, iag, iac, prg, sic, and other genes, encoding the regulator and secretory effector proteins of T3SS1. It is worthwhile to mention that not all genes within SPI-1 are associated with the T3SS1, but it has now been demonstrated that at least 29 T3SS1 genes are involved in different encoding functions. The regulators and effectors of T3SS1 are related to Salmonella colonization and invasion into intestinal epithelial cells and lead to necrosis and inflammatory reactions in macrophages [77,109]. Furthermore, these effectors are implicated in regulating the host cell exocytosis, interfering with host signal transduction pathways, and allowing Salmonella localization, survival and proliferation inside the vacuoles [95,110,111]. In addition, four genes, i.e., sit A, sit B, sit C, and sit E, play an important role in full virulence [112].
SPI-2 contains more than 40 genes which constitute four operons. From these, ssa encodes the T3SS2 [101], ssr encodes a secretion system regulator [113], and ssc encodes a molecular chaperone [114,115]. SPI-2-related secretion system T3SS2 delivers more than 20 effectors through the vacuole membrane into the host cytosol [100], playing an essential role during the second stage of host invasion which controls the survival and replication of Salmonella in phagocytes and epithelial cells [116]. At the same time, it allows Salmonella to escape the bactericidal effects of macrophages, and plays an important regulatory role in the progression of systemic infection and intracellular pathogenesis [74,116]. SPI-3, involved in the survival of Salmonella in macrophages, is around 17 kb and contains 10 ORFs constituting six transcription units. The major virulence gene encoded by SPI-3, mgtCB, is a high-affinity Mg 2+ uptake system which is required for adaptation to nutritional limitations of the intra-phagosomal habitat [117]. From these, SPI-3 has been implicated in mediating the survival of Salmonella in macrophages and low Mg 2+ environments [71].
SPI-4 is a 27 kb region that encodes a type 1 secretion system (T1SS), contributing to the adhesion of Salmonella to epithelial cell surfaces [118,119]. The SPI-4-encoded T1SS consists of five proteins (SiiABCDF) and secretes the giant adhesin SiiE, which is the largest protein in Salmonella, resulting in membrane ruffle formation and uptake of Salmonella [120,121].
SPI-6 is approximately 59 kb and encodes a type 6 secretion system (T6SS) [135]. SPI-6 T6SS contributes to intra-macrophage survival and successful establishment of S. enterica in host gut during infection [136]. The transcriptional repression of the SPI-6 T6SS core component clpV resulted in defective intra-macrophage survival, attenuated virulence, and diminished systemic dissemination [137]. SPI-7 is the largest genomic island around 134 kb in length and encodes important virulence genes, including the major Vi antigen and IV B operon in serovars Typhi, Paratyphi C and some strains of serovar Dublin [130]. These genes benefit bacteria against phagocyte-mediated killing and modulating the innate immune responses [138].
SPI-8 is approximately 6.8 kb region located adjacent to the pheV tRNA gene and encodes a degenerate integrase, two bacteriocin pseudogenes, and intact genes encoding proteins conferring resistance to these bacteriocins [133]. It has been speculated that proteins encoded in SPI-8 could improve bacterial fitness of typhoid serovars in human gut, however, further focused studies are required to support this caveat [133,134].
SPI-9 is about 16 kb island and encodes the type I system that helps in modulation of bacterial adhesion to the epithelial cells similar to the SPI-4 [129]. SPI-10 is approximately 32.8 kb in length, containing the sefB, sefC, sefR, and prpZ genes, which are implicated in the regulation of chaperone protein on mycelia manipulators [125]. From these, prpZ has been implicated in promoting S. Typhi survival in human macrophages [139,140].
SPI-11 includes pagC, pagD, and msgA, which reportedly have important roles related to the survival of S. Typhi in macrophages [141,142]. RaoN, a small RNA encoded within SPI-11, has been shown to be necessary for survival under in vitro stress conditions and contributes to the growth of S. Typhimurium in macrophages [128]. SPI-12, located next to the proL tRNA gene, is approximately 15 kb in S. Typhimurium and 6.3 kb in S. Typhi [33]. Regulation of genes within SPI-12 is conducive to in vivo adaptability [127]. SPI-13 is a 19 kb gene cluster and contributes to the virulence of Salmonella [143]. Recent studies have shown that SPI-13 mediated d-glucuronic acid (DGA) and tyramine (TYR) metabolic pathways can afford nutritional fitness to Salmonella Enteritidis (S. Enteritidis) [143].
SPI-14 is approximately 9 kb and specific to S. Typhimurium. LoiA, a novel virulence-regulating protein encoded in SPI-14, has been shown to be induced under low oxygen conditions and can enhance the ability of S. Typhimurium to invade host epithelial cells [144,145]. SPI-15, 16, and 17 were identified by bioinformatics in 2006 [146]. Studies on these islands are still very limited. SPI-15 is 6.5 kb, inserted near glyU tRNA, and is only present in S. Typhi, and absent in S. Typhimurium [146]. SPI-16 is found in S. Typhimurium and S. Typhi as a 4.5 kb fragment inserted next to argU tRNA. It is required for intestinal persistence of S. Typhimurium in mice [147]. Comparatively, SPI-17 is 5.1 kb long, and inserted in argW tRNA encoding six open reading frames (ORFs) [146]. SPI-18 harbors two ORFs organized into an operon, hlyE and taiA genes, and both are implicated in virulence. TaiA is a novel invasin involved in an increased phagocytosis of S. Typhi by macrophages [148]. HlyE presents a complex regulation network which participates in different stages of infective process. It affects the Ca 2+ homeostasis in epithelial cells by induction of slow, intracellular Ca 2+ oscillations to control S. Typhi growth in cells [149].

Molecular Mechanisms of Salmonella Immune Escape
In healthy individuals, the host body can recognize and clear pathogens through the innate and acquired immunity by a strong host immune response. However, invasive Salmonella can evade the immune surveillance using the sophisticated strategies, and could replicate, survive, and cause the persistent bacterial infections in hosts without even exhibiting the typical clinical symptoms [150]. For example, it has been reported that, in certain cases, patients with typhoid fever may carry bacteria in their gallbladder for the rest of their lives [32]. In general, such infections do not show clinical symptoms, but are a potential threat to the host. These asymptomatic carriers presumably act as reservoirs for a diverse range of S. Typhi strains and may act as a breeding ground for new genotypes [25]. It has been reported that S. Typhi chronic infection facilitates the gallbladder cancer development in humans [151]. S. Typhimurium involved in the persistent infections is also difficult to eliminate, and infected patients often continue shedding these pathogens in the environment, resulting in disease transmission [25,32].

Escape of Innate Immune System
The innate immune system provides the first line of defense against invading microorganisms by inducing a variety of inflammatory and antimicrobial responses. It is also particularly important in the gastrointestinal tract, where Salmonella is first colonized, to resist against a large variety of pathogenic microorganisms. However, it is not surprising that Salmonella has evolved strategies to overcome and adapt to an inflammatory environment. Intestinal epithelial cells are a primary cellular barrier of the gut and critical for nutrient uptake [152]. The epithelial cells form a continuous intact physical epithelial barrier with interspersing tight junctions (TJs) between each cell. Salmonella may disrupt the TJs structure through SPI-1-secreted effectors resulting in an increased permeability to luminal antigens, degrading the mucosal barrier function [153]. Intestinal microflora play a crucial role in the host defense, and oral probiotics have been shown to increase intestinal antimicrobial activity and paneth cells, which are the main intestinal cells responsible for the production of immunoreactive antimicrobial peptide (AMP) [154]. This peptide helps stabilize the intestinal barrier, while promoting the stability of intestinal microbial flora. Musca domestica cecropin and JH-3 (an analog of hemoglobin peptide P3), as the novel AMPs, were recently found to have an obvious inhibitive effect on S. Typhimurium [155,156]. However, the presence of host AMPs activates the PbgA which is required to maintain PhoPQ system of S. Typhimurium, promoting remodeling of outer membrane and resistance to innate immune AMPs [157]. The transcytosis of Salmonella across the gut epithelium by M cells is important for the induction of efficient immune responses to mucosal antigens in the Peyer's patches [158]. M cells function as the antigen-sampling cells, selectively transporting Salmonella antigens and delivering the latter to the underlying lymphoid tissues where protective immune responses are initiated [22,159]. Paradoxically, Salmonella exploit M cells as a route for the host invasion. Both S. Typhi [160] and S. Typhimurium [161] selectively target and invade M cells through SPI-1.
During Salmonella invasion of the host cells, its surface pathogen-associated molecular patterns (PAMPs) are recognized by the host cell pattern recognition receptors (PRRs) [152]. The PAMPs which are significantly expressed by Salmonella include: Lipoprotein, curli amyloid fibrils, lipopolysaccharide (LPS), flagellin, and CpG DNA, which are recognized by PRRs. In addition to identifying the PAMPs, PRRs can also recognize the "danger-associated molecular patterns" (DAMPs). During an invasive Salmonella infection, innate immune responses are initiated by PAMPs and DAMPs, leading to the activation and recruitment of neutrophils and macrophages.
Extensively studied PRRs include the Toll-like receptors (TLRs) and NOD-like receptors (NLRs) [162][163][164]. TLRs recognize Salmonella on the cell surface and in endosomes, whereas NLRs detect Salmonella components in the cytosol. In an early stage of Salmonella infection, recognition of ligands by TLRs increases the bactericidal activity of local tissue macrophages, induces the maturation and migration of dendritic cells, and initiates the production of inflammatory cytokines and chemokines [165]. Curli amyloid fibrils are recognized by the TLR2/TLR1 heterodimer complex. It was shown that inability to produce curli fibrils will markedly reduce the ability of HeLa cells to respond to stimulation with intact S. Typhimurium [166]. Moreover, epithelial cells augment the barrier function via recognizing S. Typhimurium curli fibers in the gut by activating TLR2/phosphatidylinositol 3-kinase (PI3K) pathway [167]. In addition to curli fibrils, intact Salmonella contain triacyl lipoproteins that also stimulate responses through the TLR2 receptor [166,168]. TLR4 directly recognizes LPS, one of the main components of Salmonella's outer membrane, promotes proinflammatory cytokine production, and phagocytic cell recruitment [169]. It is known that LPS is not homogeneous [170]. Additionally, studies have found that structural and chain length differences in LPS between serotypes of Salmonella are sufficient to drive different host immune responses [171][172][173]. S. Typhimurium uses PbgA and PmrA/Pmrb system to influence LPS assembly and drive variable host Type I IFN responses for their survival in various ecological niches [157,[173][174][175]. The flagellin and non-methylated CpG sequence in Salmonella DNA are easily recognized by TLR5 [176] and TLR9 [177,178], respectively. Following ligand binding, TLRs engage the signaling adaptors MyD88 and TRIF, which are recruited in the C-terminal domain of TLRs. This recruitment initiates the downstream signaling and subsequently induces the host cells to produce inflammatory factors (interleukin-8, interleukin-10, interferon-α, and others), causing an infiltration of neutrophils to the site of infection and thereby producing an inflammatory response [179]. However, it has been demonstrated that S. Typhi can prevent neutrophil recruitment in the intestinal mucosa by masking its surface antigens with SPI-7 and interfering with TLRs [138,180,181]. Moreover, a SPI-7-encoded regulatory protein TviA can reduce TLR5-mediated inflammatory responses by controlling capsule expression and flagellar movement (Figure 1) [182,183]. These evidences indicate that the encoding genes locus SPI-7 in S. Typhi is a necessary factor for escaping the host inflammatory reactions. Capsules in S. Typhimurium are wrapped around LPS, which also prevents the inflammatory response induced by TLR4 recognition [181]. Even if TLRs successfully identify the PAMPs, Salmonella SPI-2 encoded proteins, i.e., SseL, SpvD, PipA, GogA, GtgA, SpvC, can inhibit the nuclear factor kappa beta (NF-κB), extracellular signal-regulated kinase (Erk), and mitogen-activated protein kinase (MAPK) activation, thus suppressing the transcriptional responses leading to inflammation ( Figure 1) [85,106,[184][185][186]. SseL acts as a deubiquitinase and prevents the ubiquitination of IkB-α. It results in the inability of IκB-α to dissociate from NF-κB, leaving NF-κB in an inactive state [106]. SpvD targets the NF-κB pathway by interfering with nuclear translocation of p65 [184]. PipA, GogA, and GtgA redundantly target components of NF-κB signaling pathway to inhibit transcriptional responses leading to inflammation [86]. SpvC removes phosphate groups of Erk and p38 MAPKs by phosphothreonine lyase to interfere with the downstream signaling pathways [185,186]. SseK suppresses TNF-α-induced, but not TLR-induced NF-κB, activation and cell death during macrophage infection [105]. Moreover, the effector AvrA transcribed by SPI-1 is able to stabilize the intestinal epithelial permeability and tight junctions of intestinal epithelial cells to mitigate a destructive effect produced by other SPI-1 effectors (i.e., SopB, SopD, SopE, and SopE2) (Figure 1). It was shown that disintegration of tight junctions in the intestinal epithelial cells could enhance the intestinal inflammatory responses. Thus, Salmonella can also avoid the host inflammatory responses through AvrA [84].
Moreover, Salmonella can trigger their own phagocytosis by macrophages [187][188][189][190] and become encapsulated in SCV. The effector SipA, SseJ, SopE2, and SopB are required for biogenesis and correct localization of SCV [80]. SipA provides functional continuity between forced bacterial entry and the intracellular replicative niche by priming the SCV, and the localization of SseJ maintains the membrane integrity and stability of SCV [91,104]. SopB is essential for efficient cytosolic proliferation of Salmonella (Figure 1) [191]. Once Salmonella become established within SCV, they become hidden from many extracellular detection mechanisms. SseF and SseG anchor SCV at the Golgi network and remain in this region during first few rounds of bacterial replication, forming a clustered microcolony of vacuoles ( Figure 1) [192]. However, macrophages have evolved NLRs that can recognize the presence of PAMPs in the cytosol [164]. Upon binding to the ligand, the NLRs initiate different signaling cascades. NOD1 and NOD2 interact with a common adaptor protein called receptor-interacting protein 2 (RIP2) to mediate an efficient clearance of Salmonella from mucosal tissue [193,194]. Inflammasome assembly is usually triggered by the cytosolic NLRs which sense dangerous signals. It consists of NLRs, the adaptor proteins apoptosis-associated speck-like protein containing a CARD (ASC) and the effector molecules caspase-1, resulting in caspase-dependent secretion of mature pro-inflammatory cytokines IL-1β, IL-18, and pyroptotic cell death [195,196]. Mouse NLR apoptosis inhibitory protein (NAIP2) and human NAIP can recognize the S. Typhimurium T3SS inner rod component PrgJ, and NAIP5 can recognize S. Typhimurium flagellin D0 domain to induce NLR family CARD-domain containing protein 4 (NLRC4) phosphorylation and caspase-1 activation [197][198][199]. SCV lysis releases bacterium into the macrophage cytosol, where it is detected by the noncanonical inflammasome and eventually induces the pyroptotic death of the host cell [200,201]. However, SPI-2-mediated T3SS2 secrets effectors into the cytoplasm, and these effectors protect against the harmful environmental factors by regulating the vacuoles and intracellular biochemical reactions to facilitate the survival and replication of Salmonella in SCV [82,83,86,87,89,92]. Studies have demonstrated that human macrophage death and IL-1β production are elicited by S. Typhimurium SPI-1 but suppressed by SPI-2 [87,202]. SPI-2 supports the SPI-1-driven active infection of human macrophages and intra-macrophage bacterial survival [202].
Another potential reason that Salmonella induces its own phagocytosis by macrophages may be to avoid the phagocytic killing by neutrophils. This is also supported by the fact that Salmonella has a limited ability to resist the neutrophil-mediated bactericidal effects. Lysosomes in phagocytic cells contain a variety of hydrolases for combating bacteria. Evading lysozyme degradation is an important strategy for the survival of intracellular bacteria. It has been reported that SCV can fuse with lysosomes [203,204]. Interestingly, a Salmonella effector SifA, which is required to maintain the SCV membrane, has the ability to reduce the lysosomal enzyme activity (Figure 1) [90]. In addition to SifA, Salmonella also uses SopD2 to interfere with endosome-to-lysosome trafficking (Figure 1) [96]. Therefore, in order to efficiently kill pathogens in SCV, host cells are required to generate a stronger bactericidal environment.
Oxidative bursting catalyzed by nicotinamide adenine dinucleotide phosphate (NADPH) oxidase is induced by phagocytic cells to produce a large number of reactive oxygen intermediates (ROI), such as O 2 and H 2 O 2 , which are converted into a strong oxidant hypochloric acid and rapidly kill Salmonella [205]. However, Salmonella depends on SPI-2 effectors, i.e., SseB, SsrA, SsaJ, and Ssav for preventing an interaction of NADPH oxidase subunit Cytb558 with SCV to avoid the oxidative burst ( Figure 2) [102,206]. In addition, Salmonella can resist the oxidative killing effect of ROI using catalase, antioxidant proteins, and superoxide dismutase [207]. Reactive nitrogen intermediates (RNI) include nitric oxide and its derivatives, such as nitrososulfur compounds, nitrogen peroxide, etc. Reactive nitrogen intermediates can also kill Salmonella through various mechanisms, such as by causing DNA damage, preventing SPI-2 transcription, and inhibiting the PhoP/PhoQ acid-tolerance regulation reaction [208]. However, Salmonella also possesses the NO 2-operating system and nitrate reductase for protection against RNI damage [209]. Eswarappa and colleagues have shown that most SCVs in macrophages contain only one bacterium. Salmonella replicates in the SCV, and with an increasing bacterial number, one SCV divides into multiple SCVs, which benefits the survival of the intracellular bacteria [210]. On the one hand, it becomes much more difficult for the host cells to combat multiple SCVs compared to a single SCV, as this effort also requires more bactericidal media. In addition, a bacterium occupying a single SCV reduces the competition for nutrients and secretes effectors more efficiently into the cytoplasm [210]. Macrophages provide a safe haven to Salmonella for its survival and proliferation. However, when nutrients in the host cells are depleted, Salmonella is forced to induce the host cell death and search for new a host instead. Salmonella mediates macrophage death through two mechanisms [211]. One of these mechanisms involves the rapid induction of macrophage death. Salmonella expressing the SPI-1 T3SS rapidly trigger caspase-1-dependent apoptosis of infected macrophages [94,212,213]. Murine bone marrow-derived macrophages undergo lysis within 1 h of infection [214]. This rapid activation of programmed macrophage cell death depends on SPI-1 encoded protein SipB, bacterial flagellin, and the T3SS1 export machinery [211,215]. However, the other mechanism is SPI-1-independent, and characterized by a delayed induction of apoptosis to kill infected macrophages as late as 18 h post-infection. A functional T3SS2 and OmpR (ancestral regulator involved in the expression of ssrAB operon located in SPI-2) are required for the delayed induction of apoptosis, and allow Salmonella to spread intercellularly within apoptotic bodies [211,216,217]. Furthermore, past studies have indicated that the rapid and delayed activations of programmed macrophage cell death are independent of each other, since the mutations in SPI-1 do not affect the delayed induction of apoptosis, and the mutations in SPI-2 do not affect rapid induction of apoptosis [211]. The dead or dying macrophages containing Salmonella are engulfed by other macrophages recruited at the site of infection, and these macrophages can again serve as a safe haven for Salmonella to survive, while avoiding the extracellular host defenses [218]. Furthermore, studies have revealed that there are subpopulations of vacuolar and cytosolic Salmonella [219]. Vacuolar Salmonella are T3SS2-induced, whereas cytosolic Salmonella are induced by T3SS1 and flagellated [220]. The release of bacteria from SCV leads to their transcriptional reprogramming and a robust replication in the cytosol that exceeds their replication rate in the SCV [220,221]. However, the permissiveness of Salmonella survival and replication following vacuole lysis is dependent upon the cell type. For instance, Salmonella eventually hyper-replicate in the cytosol of epithelial cells but not in the cytosol of fibroblasts or macrophages [222]. Epithelial cells infected with Salmonella trigger an acute intracellular amino acid starvation, resulting in the induction of xenophagy to protect the host cells from Salmonella, but it is temporally restricted and not absolute [219,223]. Eventually, epithelial cell death via pyroptosis results in cell lysis, proinflammatory cytokine release, and escape of the cytosolic bacteria into the extracellular space, providing a potential mechanism of dissemination [219].

Escape of Adaptive Immune Responses
As the antigen presenting cells, macrophages and dendritic cells (DCs) can directly recognize the PAMPs in bacteria and present the bacterial antigens to T cells, initiate the proliferation and differentiation of naive T cells into the effector T cells, playing an important role in adaptive immune response against the invading bacteria. Interference with these functions is likely to increase the survival chances and invasion of bacteria in the hosts. Therefore, it seems that manipulating the antigen presentation capability of antigen presenting cells is another important strategy of pathogens for suppressing and escaping the host immune responses [224]. SseI has been shown to block the migration of DCs to lymphocytes [225]. Moreover, the major histocompatibility complex (MHC) plays an important role in combating the Salmonella during the later stages of infection [226,227]. It was shown that in human cells harboring intracellular Salmonella, SPI-2-encoded SifA is responsible for interfering with major histocompatibility complex class II (MHC II) cell surface expression and thereby provides Salmonella with a specific mechanism to evade or delay the host adaptive immune response ( Figure 2) [228]. SPI-2-encoded SteD with its chaperone SrcA can force an inappropriate ubiquitination of MHC II to suppress T cell activation ( Figure 2) [229,230]. Tobar and colleagues have shown that Salmonella prevents the degradation of lysosomes in DCs by SPI-2 effector SpiC, making it impossible for DCs to bind and present antigens to MHC, thus preventing the differentiation of naive T cells [231] ( Figure 2). Once T cells are activated during infection, the majority of both CD4+ and CD8+ T cells have acquired an activated phenotype and an unexpectedly large fraction of these T-cell populations secreted IFN-γ to inhibit bacterial replication [232][233][234][235]. IL-12 has been identified as a major IFN-γ inducer. Interestingly, persons lacking the IL-12 receptor are more susceptible to Salmonella infection [236,237]. TNF-α also controls S. Typhimurium replication levels in persistently infected hosts [234]. Despite a profound activation of both CD4+ and CD8+ populations, expansion of either T-cell population was marginal [238]. Only a moderate (two-to three-fold) expansion of these T-cell populations were observed over several weeks of infection. Salmonella induces the expression of inducible nitric oxide synthase (iNOS) by SPI-2 to inhibit the proliferation and differentiation of T cells [224]. Furthermore, in mice models, S. Typhimurium infection resulted in immunosuppression by increasing IL-10 and nitric oxide (NO) production with immunosuppressive activity [233,[239][240][241] (Figure 2).
Both CD4 + and CD8 + T lymphocytes and the humoral immune responses are required to control Salmonella infection [242][243][244][245][246][247][248]. It has been demonstrated that mice continuously infected with Salmonella have higher antibody titers [232] including IgA, IgM, and IgG [232,249], indicating that B cells also play an important role in the host defense. This may represent a deliberate shift from Th1/Th17 to Th2 responses [250]. Moreover, the adaptive immune responses also provide a positive feedback to the innate immune system [251]. This feedback is mediated via cytokines synthesis, leading to an increased number and activation of effector cells, and subsequently producing an increased antimicrobial response.

Perspectives
In recent years, different disciplines, such as immunology, microbiology, and cell biology have contributed greatly to our understanding of the interaction between Salmonella and the host. Several studies have revealed complex interactions between microbial pathogens and higher organisms. Future studies will hopefully expand our understanding of an interplay between immunity and bacteria in different infected organs. At present, our understanding of the interaction of Salmonella with innate and adaptive immunity evading the host defense strategies in humans is still incomplete. When the effects of normal microbial colonization flora contained in the host and the diversity of environmental conditions are analyzed, the complexity of the interaction between bacteria and host becomes far greater than our current knowledge in this domain. Invasive diseases caused by Salmonella remain a major factor accounting for the severe death and morbidity rates worldwide. Therefore, the ongoing research focusing on the relationship between Salmonella and the host immunity has the desired potential to explicate complex questions related to the Salmonella-host interactions and improve the prevention and treatment strategies aimed at combating these infectious diseases in the near future.