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Open AccessArticle

In Vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk

1
Department of Food Science and Technology, Government College Women University, Faisalabad 38040, Pakistan
2
Barani Agricultural Research Institute, Chakwal 48800, Pakistan
3
National Institute of Food Science and Technology, Faculty of Food, Nutrition and Home Sciences, University of Agriculture, Faisalabad 38040, Pakistan
4
Department of Food Science and Technology, Khwaja Fareed University of Engineering and Information Technology, Rahem Yar Khan 64200, Pakistan
5
Department of Clinical Nutrition, Nur International University, Lahore 54000, Pakistan
6
University College of Agriculture and Environmental Sciences, Islamia University, Bahawalpur 63100, Pakistan
*
Author to whom correspondence should be addressed.
Microorganisms 2020, 8(3), 354; https://doi.org/10.3390/microorganisms8030354
Received: 27 December 2019 / Revised: 15 February 2020 / Accepted: 16 February 2020 / Published: 2 March 2020
(This article belongs to the Collection Feature Papers in Food Microbiology)
The present study was designed to isolate Bifidobacterium strains from raw camel milk and to investigate their probiotic characteristics. Among 35 isolates, 8 were identified as Gram-positive, catalase negative, non-spore forming, non-motile and V or Y shaped rods. B-2, B-5, B-11, B-19 and B-28 exhibited good survival at low pH and high bile salt concentration. Most of the isolates were resistant to nalidixic acid, fusidic acid, polymyxin B, neomycin, streptomycin, gentamicin, rifampicin and kanamycin. Furthermore, the production of exopolysaccharides (EPS), adhesion characteristics, antioxidant properties, antagonistic activities, nitrite reduction and cholesterol assimilation were also studied. Isolate B-11 was chosen because it exhibited most of the probiotic properties among all the tested isolates. It is identified as the member of Bifidobacterium longum group through 16S rRNA gene sequencing and named as B. longum B-11. B. longum B-11 was further selected for in vivo attachment to rat intestine and scanning electron micrographs revealed that attachment of a large number of rods shaped bacterial cell. Our findings suggest that B. longum B-11 processes excellent attributes to be used as potential probiotic in the development of functional probiotic food. View Full-Text
Keywords: probiotic potential; antimicrobial activity; camel milk: B-11; pathogens probiotic potential; antimicrobial activity; camel milk: B-11; pathogens
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MDPI and ACS Style

Yasmin, I.; Saeed, M.; Khan, W.A.; Khaliq, A.; Chughtai, M.F.J.; Iqbal, R.; Tehseen, S.; Naz, S.; Liaqat, A.; Mehmood, T.; Ahsan, S.; Tanweer, S. In Vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk. Microorganisms 2020, 8, 354. https://doi.org/10.3390/microorganisms8030354

AMA Style

Yasmin I, Saeed M, Khan WA, Khaliq A, Chughtai MFJ, Iqbal R, Tehseen S, Naz S, Liaqat A, Mehmood T, Ahsan S, Tanweer S. In Vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk. Microorganisms. 2020; 8(3):354. https://doi.org/10.3390/microorganisms8030354

Chicago/Turabian Style

Yasmin, Iqra; Saeed, Muhammad; Khan, Wahab A.; Khaliq, Adnan; Chughtai, Muhammad F.J.; Iqbal, Rabia; Tehseen, Saima; Naz, Saima; Liaqat, Atif; Mehmood, Tariq; Ahsan, Samreen; Tanweer, Saira. 2020. "In Vitro Probiotic Potential and Safety Evaluation (Hemolytic, Cytotoxic Activity) of Bifidobacterium Strains Isolated from Raw Camel Milk" Microorganisms 8, no. 3: 354. https://doi.org/10.3390/microorganisms8030354

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