Peroxynitrite Generation and Increased Heterotrophic Capacity Are Linked to the Disruption of the Coral–Dinoflagellate Symbiosis in a Scleractinian and Hydrocoral Species

Ocean warming is one of the greatest global threats to coral reef ecosystems; it leads to the disruption of the coral–dinoflagellate symbiosis (bleaching) and to nutrient starvation, because corals mostly rely on autotrophy (i.e., the supply of photosynthates from the dinoflagellate symbionts) for their energy requirements. Although coral bleaching has been well studied, the early warning signs of bleaching, as well as the capacity of corals to shift from autotrophy to heterotrophy, are still under investigation. In this study, we evaluated the bleaching occurrence of the scleractinian coral Mussismillia harttii and the hydrocoral Millepora alcicornis during a natural thermal stress event, under the 2015–2016 El Niño influence in three reef sites of the South Atlantic. We focused on the link between peroxynitrite (ONOO−) generation and coral bleaching, as ONOO− has been very poorly investigated in corals and never during a natural bleaching event. We also investigated the natural trophic plasticity of the two corals through the use of new lipid biomarkers. The results obtained first demonstrate that ONOO− is linked to the onset and intensity of bleaching in both scleractinian corals and hydrocorals. Indeed, ONOO− concentrations were correlated with bleaching intensity, with the highest levels preceding the highest bleaching intensity. The time lag between bleaching and ONOO− peak was, however, species-specific. In addition, we observed that elevated temperatures forced heterotrophy in scleractinian corals, as Mu. harttii presented high heterotrophic activity 15 to 30 days prior bleaching occurrence. On the contrary, a lower heterotrophic activity was monitored for the hydrocoral Mi. alicornis, which also experienced higher bleaching levels compared to Mu. hartii. Overall, we showed that the levels of ONOO− in coral tissue, combined to the heterotrophic capacity, are two good proxies explaining the intensity of coral bleaching.

cause irreversible damages to biomolecules [21]. Although the implication of NO in coral bleaching has been previously studied in laboratory experiments [14,15,18,22,23], its role in the production of ONOO − , during natural bleaching events remains unclear [16].
In this field work, we specifically studied the bleaching occurrence of two coral species, the scleractinian Mussismillia harttii and the hydrocoral Millepora alcicornis, during a natural bleaching event occurring during the 2015-2016 El Niño. To further our knowledge on the role of heterotrophy and ONOO − generation in bleaching susceptibility, we followed these two parameters, together with seawater temperatures, in three different reef sites located in the South Atlantic. This is the first attempt to link ONOO − production and in situ coral bleaching, as well as to assess in situ heterotrophy of a scleractinian and hydrocoral using the PTMI index. Repeated surveys of a particular location are typically less spatially comprehensive than stratified random surveys, however, are considered a useful sampling design to provide information on stress and degradation of coral reefs [24]. Therefore, the repeated surveys in representative sections of the Recife de Fora Marine Protected Area was considered the best approach, considering the temporal scale of our study and the aim to assess physiological responses in corals. The sites were selected according to the National Oceanic and Atmospheric Administration (NOAA) predictions for coral bleaching occurrence (https://coralreefwatch.noaa.gov/satellite/analyses_guidance/global_coral_bleaching_2014_ 17_status.php). In addition, they are included in the Brazilian Reef Check monitoring program (www.reefcheck.org, www.recifescosteiros.org.br), and are part of a recent Brazilian Conservation Plan for Endangered Species of Reef Environments (PAN Corais, ICMBio, 2016). Mussismilia harttii and Mi. alcicornis are the most abundant corals in these sites (unpublished data, E. N. Calderon) and were chosen in this study.

Reef Sites
Three reef sites located at the conservation area of the Municipal Natural Park of Recife de Fora (Porto Seguro, Bahia, Northeastern Brazil) were surveyed: (1) Taquaruçu (16° 24′ 25.3"S, 38° 58′ 41.0"W); (2) Mourão (16° 24′ 39.0"S, 38° 59′ 06.1"W); and (3) Funil (16° 25′02.6"S, 38° 58′ 55.8"W) ( Figure 1). Repeated surveys of a particular location are typically less spatially comprehensive than stratified random surveys, however, are considered a useful sampling design to provide information on stress and degradation of coral reefs [24]. Therefore, the repeated surveys in representative sections of the Recife de Fora Marine Protected Area was considered the best approach, considering the temporal scale of our study and the aim to assess physiological responses in corals. The sites were selected according to the National Oceanic and Atmospheric Administration (NOAA) predictions for coral bleaching occurrence (https://coralreefwatch.noaa.gov/satellite/analyses_guidance/global_coral_bleaching_2014_17_statu s.php). In addition, they are included in the Brazilian Reef Check monitoring program (www.reefcheck.org, www.recifescosteiros.org.br), and are part of a recent Brazilian Conservation Plan for Endangered Species of Reef Environments (PAN Corais, ICMBio, 2016). Mussismilia harttii and Mi. alcicornis are the most abundant corals in these sites (unpublished data, E. N. Calderon) and were chosen in this study.

Thermal Stress Assessment
Local temperature conditions were assessed using in situ temperature data loggers (HOBO Pendant model UA-002-64) and historical data records obtained from NOAA′s 5 km virtual station, Recife de Fora, Brazil. In situ water temperature was continuously recorded every 15 min throughout the monitoring period (15, December, 2015to 15, June, 2016. Data processing followed methods developed by NOAA Coral Reef Watch (CRW) program and ReefTemp monitoring system [25]. Data obtained from the NOAA virtual station for March 2013 to December 2015 were combined with in situ temperature data from December 2015 onwards, to calculate: (i) the onset of potential coral bleaching, which was defined to begin at degree heating weeks (DHW) values of 4 °C -weeks or

Thermal Stress Assessment
Local temperature conditions were assessed using in situ temperature data loggers (HOBO Pendant model UA-002-64) and historical data records obtained from NOAA s 5 km virtual station, Recife de Fora, Brazil. In situ water temperature was continuously recorded every 15 min throughout the monitoring period (15, December, 2015to 15, June, 2016. Data processing followed methods developed by NOAA Coral Reef Watch (CRW) program and ReefTemp monitoring system [25]. Data obtained from the NOAA virtual station for March 2013 to December 2015 were combined with in situ temperature data from December 2015 onwards, to calculate: (i) the onset of potential coral bleaching, which was defined to begin at degree heating weeks (DHW) values of 4 • C-weeks or greater; and (ii) sea surface temperature anomaly (SSTA), calculated as the positive deviations of daily average temperatures from the long-term (4 years) average temperature observed for each month. DHW are the accumulation of temperature anomalies exceeding the maximum of the monthly mean SST for a given region over the last 12-week period [26][27][28]. To calculate DHW, only anomalies ≥1 • C were considered [29].

Biological Survey
Colonies of the scleractinian coral Mu. harttii and the hydrocoral Mi. alcicornis were surveyed by SCUBA diving for changes in colony coloration every 15 days, from December 15th, 2015 to June 15th, 2016. One transect of 20 × 2 m, at a depth of 2 m (low tide), was haphazardly fixed each time and performed in each of the three sites. Colonies were photographed next to a PVC Coral Health Chart [30] along the fixed transects. The number of Mu. harttii and Mi. alcicornis colonies photographed in each of the transects varied from 8-34 and 18-33, respectively. Bleaching occurrence was evaluated following the modified coral watch protocol (www.reefquest.org). Colonies that were inside (at least in part) of each transect (20 × 2 m) were recorded, and coral colonies were considered "bleached" if at least 50% of its surface area was bleached. In addition, biological samples from both species were collected every 15 days for each site alongside all transects within an area of 0.04 km 2 . For each transect, 4 colonies per species were sampled, with one polyp for Mu. harttii and an apical piece of approximately 3 cm for Mi. alcicornis. Completely bleached colonies were avoided since they tend to loose tissue biomass (e.g., protein total content), which could affect biochemical assays conducted in the present study. Fragments were cut and immediately frozen in liquid nitrogen for subsequent analyses. Collections were performed under the permission of the Brazilian Environmental Agency (SISBIO).

Fatty Acids Extraction and Analysis
Mussimilia harttii and Mi. alcicornis fragments were weighed and lipid extraction was performed according to Mies et al. [31]. Coral tissues were crushed [32] and incubated with 50 µL of tridecanoic acid (C13:0, 1.0 mg mL −1 ), 1.85 mL of methanol, and 100 µL of acetyl chloride for 1 h at 100 • C. Hexane was added, the sample vortexed, and the upper organic phase transferred to a new vial. Evaporation was performed under a N flow 2 until drying and the content was then resuspended in 100 µL of hexane. The identification of stearidonic acid (SDA), docosapentaenoic acid (DPA), and cis-gondoic acid (CGA) was done by direct comparison of their retention times with 47033 PUFA Nº1 Marine Source (Sigma-Aldrich) standard mix. For each chromatogram, peaks were integrated and normalized by the internal standard. After the extraction, the skeleton fragments were washed with 2% NaClO for 2 min and dried at 63 • C for 30 h to remove organic tissue. The fragments were reweighed and both soft tissue and skeleton weight were determined.

Predominant Trophic Mode Index
To determine which feeding mode prevailed, the data for SDA, DPA, and CGA concentration were computed into the equation below, calculating the Predominant Trophic Mode Index (PTMI). The PTMI is calculated based on the ratio between the temporal variations in the content of fatty acids related to both autotrophic (SDA and DPA) and heterotrophic (CGA) feeding [10]: where autFATM = µg of (SDA + DPA) g −1 of coral soft tissue, hetFATM = µg of CGA g −1 of coral soft tissue, t 1 = initial sampling period, t 2 = next sampling period. The result is dimensionless and indicates the prevalence of autotrophy in the case of positive values and the prevalence of heterotrophy for negative values. The tissue of coral and hydrocoral samples was analyzed for ONOOconcentration using the Amplitude TM Fluorimetric Peroxynitrite Quantification Kit (AAT Bioquest ® , Sunnyvale, CA, USA). The reagent DAX-J2 TM PON Green 99, specifically reacts with ONOO − , with high selectivity over ROS and other RNS, to generate a green fluorescent product detected at Ex/Em = 490/530 nm. Briefly, approximately 0.5 cm 2 of each coral/hydrocoral sample was cut using a cutter (HD Stainless Steel Stony Coral Cutter, Ocean Wonders, USA) and homogenized in assay buffer solution (provided by the manufacturer) using a sonicator (Frequency 70 KHz, Sonaer Ultrasonics, Farmingdale, NY, USA). The remaining piece of skeleton was discarded and homogenates were centrifuged at 10,000 g (20 min, 4 • C). The intermediary phase was used to perform the assay, according to the manufacturer instructions. Coral tissue can present green autofluorescence that can increase under temperature stress depending on symbionts clade [33]. To ensure no interferences with our measurement, we performed autofluorescence measurements on thermal stressed and non-stressed corals using the same tissue homogenate dilution used to perform the present assay (see Supplementary Materials). Measurements were performed on a solid black 96-well plate using a multi-mode microplate reader (FilterMax F5, Molecular Devices). Results were normalized to the protein content of the sample, which was determined using a commercial reagent kit based on the Bradford assay (Sigma-Aldrich, ST. Louis, MO, USA). Results were expressed as peroxynitrite concentration (µM) / mg protein.

Data Presentation and Statistical Analyses
One-way ANOVAs were used to test: (i) if Mu. harttii and Mi. alcicornis shifted between feeding modes over time (PTMI data); (ii) if peroxynitrite levels significantly varied over time. If indicated, ANOVA was followed by the Student-Newman-Keuls multiple means procedure to detect statistically different (p ≤ 0.05) means. Data were checked for normality using Shapiro-Wilk's test and for homoscedasticity using Levene's test. Data were log transformed when necessary. To investigate a correlation between peroxynitrite and bleaching, and between the PTMI and sea surface temperature anomaly, a cross-correlation analysis for time series was performed. It was taken into account that data from time series tend to be autocorrelated, and that the degrees of freedom are overestimated, increasing Type I errors and the probability of detecting spurious associations when assuming a specified α value [34,35]. Thus, we corrected the number of degrees of freedom based on the sum of cross-products of the autocorrelations of tested variables over the examined lags [35,36], instead of removing the autocorrelation from the data. In the 'Results' section, Padj refers to P-value calculated using the corrected degrees of freedom. We only show the statistics for the lagged relationship with the highest correlation coefficient (R). To check if peroxynitrite levels and bleaching intensity were correlated for both species, we performed Pearson's correlation, considering data acquired from the three reef sites and from the beginning of the monitoring period up to the onset of bleaching (higher than 40%). Peroxynitrite data were standardized among sites, equaling the highest level detected to one. Also, considering the many abiotic and biotic traits collected in the present study, and that principal component analysis (PCA) is an adequate method to integrate and interpret multiple data [37], we performed a PCA aiming to identify and summarize the correlation patterns among the multiple traits (bleaching, feeding plasticity, maximum temperature, degree heating weeks, sea surface temperature anomaly, and peroxynitrite). Also, in order to confirm the temporal patterns observed, the bleaching onset and recovery observed throughout the monitoring period was used as a discrete factor ('before', 'during', and 'after' bleaching) after scaling and centralizing the log-data using the prcomp function in vegan [38] package in the R environment (R Core Team 2013). The factoextra package [39] was used to visualize graphically the data through the function "fviz_pca_biplot".

Thermal Stress and Bleaching Frequency
Seawater temperature measured during the monitoring period for each of the sites is summarized in Table 1. Sea surface temperature anomaly (SSTA) was observed throughout the monitoring period for all sites (Figures 2 and 3). SSTA positive deviations ranged from 0.1 to 1.1 • C at Taquaruçu, from 0.1 to 1.0 • C at Funil, and from 0.1 to 1.3 • C at Mourão.  Figures 2 and 3). For this locality, Mi. alcicornis showed signs of bleaching during the entire monitoring period, except for the second half of January and first half of June, while 100% bleaching was observed from mid-March to mid-May ( Figure 2). Mussismilia harttii showed bleaching signs mainly from April to May, with highest bleaching frequency (67%) from mid-April to May (Figure 2). DHW values of 6 • C-weeks or greater were observed from April to mid-June at Funil site (Figures 2  and 3). Bleaching was observed for Mi. alcicornis from March to May, with the highest bleaching frequency (86%) observed in mid-April. In turn, Mu. harttii showed bleaching signs between March (20%) and mid-May (67%) (Figure 3).
No critical thermal stress indicated as DHW was observed for the site Mourão (Figures 2 and 3), however, maximum temperatures (up to 34.2 • C) were registered for this location. Bleaching for Mi. alcicornis was observed from mid-February to May, with the highest bleaching frequency (60%) in May. Mu. harttii bleached from March to May, with the highest bleaching frequency (53%) observed in the first half of May ( Figure 3).

Predominant Trophic Mode Index
Significant shifts of PTMI were observed for Mu. harttii at all sites (ANOVA, 1.19 ≤ F ≤ 1.631, 0.001 < p ≤ 0.006, Figure 3). At Taquaruçu site, a significant predominance of heterotrophy was observed during the second half of January and February, while autotrophy was predominant during the first half of February (SNK, p < 0.001). At Funil, heterotrophy was significantly dominant during the entire month of March (SNK, p < 0.001), while autotrophy prevailed the rest of the monitoring period. At Mourão site, autotrophy prevailed during the first half of February (SNK, p = 0.05). However, it was followed by a significant heterotrophy peak in the second half of February (SNK, p = 0.006). Cross-correlation analysis shows that SSTA was associated with PTMI for Mu. harttii for all three sites (−0.783 ≤ R ≤ −0.638, 0.004 ≤ Padj ≤ 0.05, 9.4 ≤ df ≤ 10.8) with a lag of 15 days for Taquaruçu and Funil, and of 30 days for Mourão, which means that a peak in sea surface temperature anomaly preceded the heterotrophy peak by 15 or 30 days (Table 2, Figure 3). On the contrary to Mu. harttii, Mi. alcicornis displayed a near-equilibrium between feeding modes at all sites, with PTMI values not varying significantly (ANOVA, 3.62 ≤ F ≤ 12.38, 0.16 ≤ p ≤ 0.34, Figure 2). Accordingly, no correlation between PTMI and SSTA (P ≥ 0.05) was observed for all collecting sites (Table 2).
A significant correlation was observed between ONOO − levels and bleaching intensity for Mi. alcicornis  Cross-correlation analysis indicated species-specific time lags between peroxynitrite generation and bleaching for Taquaruçu and Funil. Bleaching and peroxynitrite were correlated with no lag (R = 0.808, Padj = 0.003) and a 15 days lag (R = 0.63, Padj = 0.04) for M. alcicornis from the locality of Funil (df = 7.3) ( Table 2). Peroxynitrite and bleaching were also correlated in M. harttii with a 15 days lag both at Funil (R = 0.66, Padj = 0.03,) and Taquaruçu (R = 0.75, Padj = 0.01, df = 6.8; Table 2). No correlation between peroxynitrite generation and bleaching was observed in Mourão (P > 0.05), where no cumulative thermal stress (as DHW) was detected, and a lower bleaching frequency was observed for both species.   Cross-correlation analysis indicated species-specific time lags between peroxynitrite generation and bleaching for Taquaruçu and Funil. Bleaching and peroxynitrite were correlated with no lag (R = 0.808, Padj = 0.003) and a 15 days lag (R = 0.63, Padj = 0.04) for M. alcicornis from the locality of Funil (df = 7.3) ( Table 2). Peroxynitrite and bleaching were also correlated in M. harttii with a 15 days lag both at Funil (R = 0.66, Padj = 0.03,) and Taquaruçu (R = 0.75, Padj = 0.01, df = 6.8; Table 2). No correlation between peroxynitrite generation and bleaching was observed in Mourão (P > 0.05), where no cumulative thermal stress (as DHW) was detected, and a lower bleaching frequency was observed for both species.

Discussion
This study brings new insights into the mechanisms involved in the breakdown of the cnidariandinoflagellate symbiosis during a natural bleaching event under El Niño influence. We showed that high peroxynitrite (ONOO − ) levels are associated with bleaching occurrence and intensity, with however a species-specific time lag. Also, this study brings the first evidence of a thermally induced shift from auto-to heterotrophy in the scleractinian coral Mu. hartii, while the hydrocoral Mi. alcicornis was unable to show such nutritional plasticity.

Thermal Stress, Bleaching Frequency, and Feeding Mode Plasticity
A positive correlation between large-scale bleaching (>50%-60% of the colonies investigated) and DHW was observed for both coral species in two out of the three monitored sites (Taquaruçu and Funil), in agreement with previous observations [2,29]. The third site (Mourão), which didn't experience DHW, presented a lower bleaching intensity (<60% of the colonies investigated), although still half of the coral colonies bleached during the highest thermal anomalies recorded. Such lack of correlation between bleaching and DHW was previously observed [40], and was related to the occurrence of small increases in temperature (lower than 1 °C above monthly mean) persisting over months. The comparison of the seawater temperature profiles of the three sites, however, suggests

Discussion
This study brings new insights into the mechanisms involved in the breakdown of the cnidarian-dinoflagellate symbiosis during a natural bleaching event under El Niño influence. We showed that high peroxynitrite (ONOO − ) levels are associated with bleaching occurrence and intensity, with however a species-specific time lag. Also, this study brings the first evidence of a thermally induced shift from auto-to heterotrophy in the scleractinian coral Mu. hartii, while the hydrocoral Mi. alcicornis was unable to show such nutritional plasticity.

Thermal Stress, Bleaching Frequency, and Feeding Mode Plasticity
A positive correlation between large-scale bleaching (>50%-60% of the colonies investigated) and DHW was observed for both coral species in two out of the three monitored sites (Taquaruçu and Funil), in agreement with previous observations [2,29]. The third site (Mourão), which didn't experience DHW, presented a lower bleaching intensity (<60% of the colonies investigated), although still half of the coral colonies bleached during the highest thermal anomalies recorded. Such lack of correlation between bleaching and DHW was previously observed [40], and was related to the occurrence of small increases in temperature (lower than 1 • C above monthly mean) persisting over months. The comparison of the seawater temperature profiles of the three sites, however, suggests that bleaching at Mourão may be related to the highest sea surface temperature anomaly (SSTA) recorded among sites (+1.3 • C) and to other environmental stressors such as water quality. Mourão is indeed located in the most landward side of Recife de Fora conservation reef area, showing twofold higher average levels of nitrogen (up to 1.16 µmol L −1 ) than the seaward side-Taquaruçu [41]. Due to the fact that high levels of nitrogen in seawater have been linked to reduction in the bleaching threshold [33], high nitrogen concentration at Mourão could explain the bleaching occurrence, in the absence of DHW.
Bleaching frequency was also species-specific, with Mi. alcicornis displaying twice higher bleaching frequency (up to 100%) at all sites compared to Mu. harttii. In agreement with our observation, hydrocorals were among the first reef-building species to undergo bleaching during the El Niño event (1982)(1983) of the Pacific coast of Panama [42]. In addition, a recent study on South Atlantic Ocean reefs reported Millepora spp. as the most affected species during the bleaching events of 2016 and 2017 [43]. The higher sensitivity of hydrocorals to thermal stress compared to some scleractinian coral species, as observed here and in the aforementioned studies, may be related to their lower heterotrophic capacity. While the scleractinian coral Mu. harttii significantly shifted between feeding modes, Mi. alcicornis trophic mode remained unaffected during the entire monitoring period. Previous studies have reported that corals rely on heterotrophy as a compensation mechanism for the reduced autotrophy resulting from bleaching [8][9][10]44]. As the size of corallites has already been described as a proxy for heterotrophic capacity [45], the high degree of heterotrophy in Mu. harttii may be explained by its large corallite, with a diameter of 15 mm or larger [46], coupled with large tentacles, which also favor predation [47]. This is 50-fold greater than the gastropore diameter found in Mi. alcicornis (0.3 mm) [48]. Therefore, Mu. harttii may have more resources than Mi. alcicornis for enduring thermal stress events. The shifts to heterotrophy in Mu. harttii were indeed correlated with the occurrence of sea surface temperature anomalies (SSTA). These findings are in concert with previous reports for other scleractinian coral species [10,49,50] and can be associated with variability in oceanographical and meteorological phenomena [10,51,52].

Peroxynitrite Generation and Bleaching
It has been hypothesized that the deleterious effects of high levels of nitric oxide (NO) during bleaching are related to its conversion to ONOO − , which acts as a cytotoxic compound. Specifically, ONOO − , through mitochondrial membrane damage, may be responsible for the release of potent proapoptotic molecules that initiate an apoptotic cascade involved in the bleaching process [14,15] In vivo production of ONOO − in the symbiotic sea anemone Exaiptasia pulchella indeed occurred under thermal stress; nonetheless, owing to the nonsignificant effect of ONOO − scavenger, its generation was considered insufficient to influence symbiont loss [16]. In contrast, our results bring the first evidence that ONOO − is an important mediator of bleaching in scleractinian corals and hydrocorals experiencing heat stress under field conditions. High levels of ONOO − were indeed detected shortly before the maximum bleaching frequency for both species at all sites (Figures 2 and 3) and a significant correlation was observed between the highest levels of ONOO − and the highest bleaching frequency ( Figure 4). Additionally, species-specific time lag responses were detected through cross-correlation analysis regarding maximum ONOO − generation and maximum bleaching frequency, from 0 to 30 days prior bleaching. The different time lags found for each species, greater for Mu. harttii (up to 30 days before) and shorter for Mi. alcicornis (up to 15 days before), were also reinforced by PCA that correlated ONOO − before bleaching occurrence for Mu. harttii and during bleaching occurrence for Mi. alcicornis.
The species-specific time lag response for ONOO − generation and bleaching occurrence in the present study suggests a differential susceptibility to ONOO − and/or capacity to neutralize and counteract the deleterious effects of this highly reactive RNS. For example, the antioxidant capacity has been demonstrated as a critical component of stress tolerance in scleractinian corals [53] and antioxidant capacity has been reported to be approximately threefold lower in Mi. alcicornis than in Mu. harttii [54]. Therefore, the lower antioxidant capacity observed in Mi. alcicornis can explain why this species present a smaller interval between ONOO − generation and bleaching, while Mu. harttii, with a higher antioxidant capacity, presents a repeated and longer interval between ONOO − and bleaching. In addition, these species-specific time lags and antioxidant capacities are aligned with the different bleaching frequencies observed (higher and lower for Mi. alcicornis and Mu. harttii, respectively).
Also, it is worth noting that most studies on the role of RNS in the bleaching mechanisms were performed with laboratory experiments, which could differ from the present study with corals undergoing bleaching in the field [16]. Finally, despite the fact that the present study cannot prove that alternative bleaching pathways directly involving ROS or NO are as critical as ONOO − , we propose that this highly cytotoxic molecule may exert a more relevant influence in cnidarians' thermal bleaching process than previously suggested.

Conclusions
Our findings show that ONOO − may be an important mediator of bleaching in scleractinian corals and hydrocorals experiencing heat stress under field conditions, with consistent observation of higher levels preceding maximum bleaching occurrence in a species-specific time lag. Measuring ONOO − may be an additional tool to quantitatively assess the stress level in corals, similar to chlorophyll and ROS. Further investigations are, however, needed to bring detailed insights into the mechanisms of ONOO − involved in the onset of bleaching. In addition, we show that SSTA may increase heterotrophic feeding and that the higher sensitivity of hydrocorals to thermal stress may be related to their lower heterotrophic input. The overall results may further the understanding of the biochemical mechanisms associated with bleaching, as well as of the trophic behavioral response that cnidarians may display when under thermal stress.
Supplementary Materials: The following are available online at http://www.mdpi.com/2076-2607/7/10/426/s1, Table S1: Autofluorescence data detected at Ex/Em = 490/530nm (fluorescence/mg protein, mean ± SEM, N = 6) of two coral species, the scleractinian Mussismilia harttii and the hydrocoral Millepora alcicornis, before (samples collected from December to March) and during thermal stress (April to June), and their statistical descriptors. No significant differences in fluorescence between thermal stressed and non-stressed corals, for each species, were indicated by Student's t-test.