Characterization of Growth-Promoting Activities of Consortia of Chlorpyrifos Mineralizing Endophytic Bacteria Naturally Harboring in Rice Plants—A Potential Bio-Stimulant to Develop a Safe and Sustainable Agriculture

Eighteen pesticide-degrading endophytic bacteria were isolated from the roots, stems, and leaves of healthy rice plants and identified through 16S rRNA gene sequencing. Furthermore, biochemical properties, including enzyme production, dye degradation, anti-bacterial activities, plant-growth-promoting traits, including N-fixation, P-solubilization, auxin production, and ACC-deaminase activities of these naturally occurring endophytic bacteria along with their four consortia, were characterized. Enterobacter cloacae HSTU-ABk39 and Enterobacter sp. HSTU-ABk36 displayed inhibition zones of 41.5 ± 1.5 mm, and 29 ± 09 mm against multidrug-resistant human pathogenic bacteria Staphylococcus aureus and Staphylococcus epidermidis, respectively. FT-IR analysis revealed that all eighteen isolates were able to degrade chlorpyrifos pesticide. Our study confirms that pesticide-degrading endophytic bacteria from rice plants play a key role in enhancing plant growth. Notably, rice plants grown in pots containing reduced urea (30%) mixed with either endophytic bacterial consortium-1, consortium-2, consortium-3, or consortia-4 demonstrated an increase of 17.3%, 38.6%, 18.2%, and 39.1% yields, respectively, compared to the control plants grown in pots containing 100% fertilizer. GC–MS/MS analysis confirmed that consortia treatment caused the degradation of chlorpyrifos into different non-toxic metabolites, including 2-Hydroxy-3,5,6 trichloropyridine, Diethyl methane phosphonate, Phorate sulfoxide, and Carbonochloridic. Thus, these isolates could be deployed as bio-stimulants to improve crop production by creating a sustainable biological system.


Introduction
Agriculture remains one of the most important economic sectors in Bangladesh and plays a crucial role in the rural economy of the country. Thus, the performance of this sector

Molecular Characterization and Phylogeny of Endophytic Bacteria
The genomic DNA of bacterial isolates was extracted as described by Haque et al. (2015) [14]. The amplification of 16S rRNA gene was performed using forward primer 27F 5 -AGA GTT TGA TCM TGG CTC AG-3 and reverse primer 1492R 5 -GGT TAC CTT GTT ACG ACT T-3 [15]. The primers, template DNA, and Taq DNA polymerase were added into the master mix right before loading the sample. After PCR reaction, the amplification was visualized by gel electrophoresis. After purification of the amplified 16S rRNA gene, the concentration was measured, diluted into 5 ng/µL, mixed with a ready reaction premix, and run for PCR sequences using genetic analyzer 3130 (Applied Biosystem, CL, Beverly, MA, USA). The resulting sequences were analyzed by BLASTn, submitted to NCBI, and the accession numbers were registered. The 16S rRNA sequences were compared to different 16S rRNA genes of other bacteria in the reference RNA sequences from NCBI nucleotide BLAST. The query sequences were used to create a phylogenetic tree using the neighborjoining method, where the bootstrap test included 2000 replicates [16].

FT-IR for Pesticide-Degrading Activity Confirmation
The FT-IR spectroscopy was performed as described by Pourbabaee et al. (2018) [16] to obtain information about the qualitative changes of chlorpyrifos in bacteria-treated solution. Eighteen isolates were incubated in chlorpyrifos-containing liquid media for 14 days. From each culture, 5 mL was extracted using 10 mL n-hexane on a rotary shaker for 30 min, dehydrated via anhydrous sodium sulfate (Na 2 SO 4 ), evaporated to dryness, subsequently diluted to a final volume of 5 mL with acetone, and analyzed by using FT-IR spectrophotometer in the range of 400-4000 cm −1 at 20 • C [17].

Biochemical Analysis
Biochemical tests were performed to characterize each isolate. Fresh bacterial isolates were grown on autoclaved nutrient agar media. The catalase and oxidase activities of the isolates were conducted as described [7]. Briefly, each isolate was incubated in Simmons Citrate agar medium, and a change of color from green to blue due to pH change indicated a positive reaction after incubation for 48 h at 37 • C [18]. The tests for Indole, Methyl red and Voges-Proskauer, urease, motility, triple sugar iron agar, glucose, maltose, lactose, and sucrose fermentation were performed as described [7,19]. The activities of cell wall hydrolytic enzymes, including cellulase, xylanase, pectinase, amylase, and protease, were performed in minimal nutrient agar media containing carboxymethylcellulose, oat-spelt xylan, pectin, starch, and casein powder (1%) as the sole source of carbon [15,20]. The lignin derivatives' degrading activity of the isolates was confirmed using their growth on aromatic-dye-enriched minimal nutrient media [20,21]. A test tube containing the pure culture of endophytic bacteria was inoculated with 10 mL of phenol red broth supplemented with 1 g/100 mL of various sources of carbohydrates and incubated for 24 h at 37 • C. A yellow color indicated positive reaction, and the bubbles trapped inside the Durham tube indicated gas production [18]. The pure colonies were subjected on specific substrate agar plates, as previously described [15].

Indole-3-Acetic Acid (IAA) and ACC Deaminase Production
The quantification method for IAA of the endophytes was adopted as previously described [22]. To measure the production of IAA, bacterial isolates were inoculated into 0.5 mg L-tryptophan/mL containing medium and at 37 • C with continuous shaking at 125 rpm for 48 h, as described [23]. Then, the 2 mL culture was centrifuged at 15,000 rpm for 1 min, and a 1 mL aliquot of the supernatant was mixed with 2 mL of Salkowski's reagent, incubated for 20 min in darkness at room temperature. The absorbance was measured on a spectrophotometer at 530 nm, and the concentration was determined using a standard curve of pure IAA, as previously described [22].
The ACC deaminase activity of the chlorpyrifos-degrading bacteria was determined according to the modified methods [24][25][26], which measure the amount of α-ketobutyrate produced upon the hydrolysis of ACC. The endophytic bacterial strains were separately grown in tryptic soy broth medium (TSB) for 18 h at 28 • C to determine the ACC deaminase activity as described in our study [21]. The cell suspension without ACC was used as a negative control, and the one with (NH 4 ) 2 SO 4 (0.2% w/v) was used as a positive control. The number of µmol of α-ketobutyrate produced by this reaction was determined by comparing the absorbance at 540 nm of a sample to a standard curve of α-ketobutyrate ranging between 10 and 200 µmol [24,26].

Phosphate and Nitrogen Solubilization
The N-fixation ability of the endophytic bacteria was determined by streaking isolates on the Jensen's medium. A yellow halo zone around bacterial growth after 5-7 days incubation at 37 ± 2 • C indicated positive N-fixation activity [7]. The phosphate solubilization by isolates growing in 'National Botanical Research Institute's Phosphate Growth Medium' was observed with a halo zone [7,27].

Anti-Bacterial Activity against Multidrug-Resistant Bacteria
Four different multidrug-resistant human pathogenic bacteria, including S. aureus, E. coli, Klebseilla sp., S. epidermidis, were collected from Dhaka Central International Medical College and Hospital, Dhaka, Bangladesh. Sensitivity tests of all endophytes against human pathogenic bacteria were conducted using the procedures described [15]. The diameters of the inhibition zones were measured in millimeters after 16,32, and 48 h of inoculation [15]. Since the multidrug-resistant (MDR) pathogenic bacteria did not respond to any known antibiotics or known strains within our capacity, we used Bacillus sp. Strain HSTU-10 (MG582603) as a negative control.

Rice Plant Growth-Promoting Effects of Endophytic Bacteria
The crew members of each consortium were deliberately selected to incorporate a diverse range of bacterial genera, aiming to maximize the combined effects of promoting plant growth in rice plants. The following four synthetic consortia were created using various combinations of the endophytic bacterial isolates to test the effectiveness of growthpromoting activities of the pesticide-degrading endophytic bacteria: consortium-1: Klebsiella sp. HSTU-Bk11, Acinetobacter sp. HSTU-Abk29, Citrobacter sp. HSTU-Abk30, and Enterobacter cloacae HSTU-Abk39; consortium-2: Enterobacter cloacae HSTU-Abk37, Enterobacter ludwigii HSTU-Abk40, Acinetobacter baumannii HSTU-ABK42, Klebsiella sp. HSTU-Abk31, Acinetobacter sp. HSTU-Bk12; consortium-3: Pseudomonas sp. HSTU-Bk13, Citrobacter sp. HSTU-Bk14, Acinetobacter sp. HSTU-Bk15, Acinetobacter sp. HSTU-Abk32, and Burkholderia sp. HSTU-ABK33; and consortium-4: Acinetobacter sp. HSTU-Abk34, Enterobacter HSTU-Abk36, Enterobacter sp. HSTU-Abk38, and Serratia marcescens HSTU-Abk41. All endophytes were tested for their compatibility for consortium preparation [28]. In short, each endophytic isolate was grown in nutrient broth for 24 h (10 6 CFU/mL for rice plant) and was used as inoculum. One loopful of each endophytic isolate was streaked on the opposite side of the medium in a Petri plate and then incubated at 30 • C for 48-72 h [28]. The endophytic bacterial effects on the growth promotion of rice plant genotype (Shonamukhi) were assayed at germination along with the vegetative-to-paddy yield stages. To this end, the germination test was performed with individual endophytes and their consortia in Petri plates. The effects of endophytes on the vegetative and reproductive stages were assessed with synthetic consortia.

Seed Germination Performance
Twenty-five rice seeds sterilized with 70% ethanol for 5 min and washed with distilled water five times in a septic condition were plated on a Petri dish containing sterilized 1% agar media. Autoclaved distilled water was used for all experiments. Bacterial treatment was provided individually at 100 µL/Petri dish after 24 h (approximately 10 6 ) CFU/mL, except for the control plate. Following the sowing of seeds, germination was recorded at 24 h intervals and continued up to 6 days, when the seed was considered germinated, as the plumule and radicle were >2 mm long [29]. The bacterial inoculum was prepared following a previously published method [30]. The effects of both the individual members of the bacterial consortia and the effects of each of the four consortia were recorded. The germination percentages of the seeds were calculated. The root and shoot lengths of individual seedlings were measured after 7 days of sowing [29], and the vigor index was calculated using percent germination multiplied by seedling length (shoot length + root length).

Chlorophyll Content of Fresh Leaf
Prior to measuring the chlorophyll content, the fresh leaves of rice plants were weighed. Using the spectrophotometric approach, the chlorophyll was extracted in 80% acetone, centrifuged, and the absorption of the extracts at wavelengths of 663 nm (D663) and Microorganisms 2023, 11, 1821 6 of 29 645 nm (D645) was measured. Total chlorophyll (Chl-t), chlorophyll a (Chl-a), and chlorophyll b (Chl-b) concentrations were estimated as described (Zhang et al., 2009) [31].

Root Length, Shoot Length, and Plant Height
The root length was measured from the collar region to the tip of the longest root in centimeters, while the shoot length was measured from the collar region to the tip of the shoot, and mean shoot length was expressed in centimeters. The plant heights were measured from the ground level to the tip of the topmost leaf at early stages (15, 30, 60, 90 days), up to the tip of the main panicle at maturity, and the average height was expressed in centimeters [32].

Plant Dry Matter Production
The shoots, roots, and leaves were first washed and then air-dried in the shade for 24 to 36 h prior to weighing, and the average dry weight of the plant was expressed in grams [32].

Yield Parameters (Grain Yield per Plant)
The weight of the grains in the panicles per plant from five tillers selected from randomly labeled plants was recorded, and the mean was expressed in grams [32].

Harvesting and Observations
The paddy crops were harvested after 120 days of transplantation. The germination, seedling growth parameters, plant growth parameters, plant biomass production, and yield parameters were recorded [32].

GC-MS/MS Analysis of Chlorpyrifos Degradation by Each of the Four Consortia
To ensure the consortia chlorpyrifos degradation compatibility, the chlorpyrifos (1% as carbon sources) enriched media were treated with each of the four synthetic consortia for 14 days. To perform the GC-MS analysis, 5 mL of consortia treated broth was shifted to separating funnels. Next, 25 mL of deionized water and 5 mL of n-hexane were added to the separating funnel as described [7]. After 5-10 min of shaking, the n-hexane layer with the solvents, which appeared on the upper hexane layer, was kept for further analysis with the Shimadzu GCMS-QP2010 Ultra (Japan) mass detector connected with a capillary column of Rxi-5ms, 30 m long, 0.25 mm i.d., 0.25 µm film thickness. One microliter of the sample was injected in a splitless mode, and the analyses were performed in a full scan mode, ranging from m/z 50 to 400. The compounds were detected after analyzing the mass spectrum of each component using the NIST11 library [33].

Statistical Analysis
The data were analyzed (frequency, homogeneity of variances, and LSD (ρ < 0.05)) and visualized (graph and bar chart) using SPSS, Microsoft Excel, and R language statistical software. In the bar charts, the means and error bars depict standard errors, while each letter indicates significant (ρ < 0.05) differences in plant growth parameters between treatments.

Isolation and Selection of Chlorpyrifos-Degrading Endophytic Bacteria
Forty isolates obtained from the roots, shoots, and leaves of Kalijeera (indigenous variety) and BRRI-28 rice plants were screened based on their capabilities of utilizing chlorpyrifos as their sole carbon source-a characteristic of pesticide-degrading bacteria. Out of these forty, we selected eighteen isolates by analyzing morphological (size, shape, color) data and biochemical test results. As depicted in Figure 1, all strains demonstrated noticeable growth over 12 days compared to that observed in control. riety) and BRRI-28 rice plants were screened based on their capabilities of utilizing chlorpyrifos as their sole carbon source-a characteristic of pesticide-degrading bacteria. Out of these forty, we selected eighteen isolates by analyzing morphological (size, shape, color) data and biochemical test results. As depicted in Figure 1, all strains demonstrated noticeable growth over 12 days compared to that observed in control.

Molecular Characterization of the Pesticide-Degrading Endophytic Bacteria
The vigorous mineralizing capabilities of chlorpyrifos (CPF) by five endophytic bacteria, including Klebsiella, Enterobacter, Citrobacter, Serratia, and Acinetobacter isolated from the roots of rice plants, are illustrated in Figure 2A. Two strains of Enterobacter sp. HSTU-Abk38 and HSTU-Abk36 showed substantial genetic distances and occupied different taxa in the phylogenetic tree ( Figure 2A). In particular, the strain HSTU-Abk38 was placed in a separate node located between the Klebsiella pneumoniae and Klebsiella aerogenes. Similarly, the Citrobacter sp. Strain HSTU-Abk30 was deviated to a single node from the Citrobacter freundi and Enterobacter ludwigi (Figure 2A). A very similar observation was recorded for the Serratia marcescens strain HSTU-Abk41 and Acinetobacter sp. Strain HSTU-Abk29, suggesting genetic diversities of these strains.
Four genera, Acinetobacter, Pseudomonas, Citrobacter, and Enterobacter, isolated from the shoots of rice plants ( Figure 2B), were not placed in the same node or sister taxa with reference type strains, except the Acinetobacter sp. strain HSTU-Bk15, which showed 100% similarity with the Acinetobacter soli strain B1. Notably, the Citrobacter sp. strain HSTU-Bk14 was placed in sister taxa with the Enterobacter cloacae strain HSTu-ABk39, which formed a different node from the Citrobacter and Enterobacter ( Figure 2B). Similarly, the Pseudomonas sp. strain HSTU-Bk13 occupied a single node, which was closer to the Pseudomonas aeruginosa strain DSM50071 and Pseudomonas aeruginosa strain ATCC10145. Overall, these results suggest that the chlorpyrifos mineralizing endophytes isolated from the shoots exhibit diversities but are dominated by the Enterobacter species.
The vigorous mineralizing capabilities of chlorpyrifos (CPF) by five endophytic bacteria, including Klebsiella, Enterobacter, Citrobacter, Serratia, and Acinetobacter isolated from the roots of rice plants, are illustrated in Figure 2A. Two strains of Enterobacter sp. HSTU-Abk38 and HSTU-Abk36 showed substantial genetic distances and occupied different taxa in the phylogenetic tree ( Figure 2A). In particular, the strain HSTU-Abk38 was placed in a separate node located between the Klebsiella pneumoniae and Klebsiella aerogenes. Similarly, the Citrobacter sp. Strain HSTU-Abk30 was deviated to a single node from the Citrobacter freundi and Enterobacter ludwigi (Figure 2A). A very similar observation was recorded for the Serratia marcescens strain HSTU-Abk41 and Acinetobacter sp. Strain HSTU-Abk29, suggesting genetic diversities of these strains. Four genera, Acinetobacter, Pseudomonas, Citrobacter, and Enterobacter, isolated from the shoots of rice plants ( Figure 2B), were not placed in the same node or sister taxa with reference type strains, except the Acinetobacter sp. strain HSTU-Bk15, which showed 100% similarity with the Acinetobacter soli strain B1. Notably, the Citrobacter sp. strain HSTU-Bk14 was placed in sister taxa with the Enterobacter cloacae strain HSTu-ABk39, which formed a different node from the Citrobacter and Enterobacter ( Figure 2B). Similarly, the Pseudomonas sp. strain HSTU-Bk13 occupied a single node, which was closer to the Pseudomonas aeruginosa strain DSM50071 and Pseudomonas aeruginosa strain ATCC10145. Overall, these results suggest that the chlorpyrifos mineralizing endophytes isolated from the shoots exhibit diversities but are dominated by the Enterobacter species.
A total of three genera of endophytic bacteria, i.e., Klebsiella, Burkholderia, and Acinetobacter, were obtained from the leaves of rice plants ( Figure 2C). Four strains were placed with the same taxon of Klebsiella, Burkholderia, and Acinetobacter species. The Acinetobacter baumannii strain HSTu-ABk42 was placed in sister taxa with the Acinetobacter baumannii strain ATCC 19606, while the Acinetobacter sp. strain HSTu-ABk32, Acinetobacter sp. strain HSTu-ABk34, and Acinetobacter sp. strain HSTU-Bk12 were separately placed in different nodes of the same clade. Furthermore, the Burkholderia sp. strain HSTu-ABk33 occupied a sister taxon with the Burkholderia territorii strain LMG28158, which greatly deviated from the other Burkholderia nodes ( Figure 2B). In aggregate, these results demonstrate that the chlorpyrifos mineralizing leaf endophytes of rice plants were dominated by the Acinetobacter species.  The fields were exposed to massive pesticides for rice cultivation over the last 10 years. (A) Phylogeny of root endophytic bacteria. (B) Diversity of chlorpyrifos-resistant endophytic bacteria isolated from two different rice fields. The fields were exposed to massive pesticides for rice cultivation over the last 10 years. (B) Phylogeny of shoot endophytic bacteria. (C) Diversity of chlorpyrifos-resistant endophytic bacteria isolated from two different rice fields. The fields were exposed to massive pesticides for rice cultivation over the last 10 years. (C) Phylogeny of leaf endophytic bacteria. Black color strains are reference bacteria whether different colors are isolated strains in this study.

Chlorpyrifos Biodegradation Confirmation Using FT-IR Analysis
A total of three genera of endophytic bacteria, i.e., Klebsiella, Burkholderia, and Acinetobacter, were obtained from the leaves of rice plants ( Figure 2C). Four strains were placed with the same taxon of Klebsiella, Burkholderia, and Acinetobacter species. The Acinetobacter baumannii strain HSTu-ABk42 was placed in sister taxa with the Acinetobacter baumannii strain ATCC 19606, while the Acinetobacter sp. strain HSTu-ABk32, Acinetobacter sp. strain HSTu-ABk34, and Acinetobacter sp. strain HSTU-Bk12 were separately placed in different nodes of the same clade. Furthermore, the Burkholderia sp. strain HSTu-ABk33 occupied a sister taxon with the Burkholderia territorii strain LMG28158, which greatly deviated from the other Burkholderia nodes ( Figure 2B). In aggregate, these results demonstrate that the chlorpyrifos mineralizing leaf endophytes of rice plants were dominated by the Acinetobacter species. Figure 3 illustrates the FT-IR spectrum of chlorpyrifos biodegradation observed after 14 days of incubation of the endophytic strains with minimal nutrient media (MSN) enriched with chlorpyrifos, when the C-H asymmetric vibration bond belonging to the typical methyl at 2870-2960 cm −1 and 772 cm −1 completely disappeared after bacterial treatment. The peak around 1370-1462 cm −1 indicates the C=C and C=N bonds along with the peak at 1220 cm −1 , representing the C-N bonding vibration observed in control but one that disappeared in the case of all endophyte treatments. Moreover, the peak at 1024 cm −1 assigned for C=O appeared only in untreated control samples ( Figure 3). Notably, some new peaks around 640-650 cm −1 , 1100-1120 cm −1 , and 3200-3250 cm −1 were recorded for the samples treated with endophytic strains. These results indicate that all endophytic strains belonging to the genera of Klebsiella, Enterobacter, Citrobacter, Serratia, Acinetobacter, Pseudomonas, and Burkholderia isolated from rice plants were capable of using chlorpyrifos as a carbon source. treatment. The peak around 1370-1462 cm −1 indicates the C=C and C=N bonds along with the peak at 1220 cm −1 , representing the C-N bonding vibration observed in control but one that disappeared in the case of all endophyte treatments. Moreover, the peak at 1024 cm −1 assigned for C=O appeared only in untreated control samples ( Figure 3). Notably, some new peaks around 640-650 cm −1 , 1100-1120 cm −1 , and 3200-3250 cm −1 were recorded for the samples treated with endophytic strains. These results indicate that all endophytic strains belonging to the genera of Klebsiella, Enterobacter, Citrobacter, Serratia, Acinetobacter, Pseudomonas, and Burkholderia isolated from rice plants were capable of using chlorpyrifos as a carbon source.

N-Fixation and PO4-Solubilization Activity
The nitrogen fixation activities of the pesticide-degrading endophytic bacteria were assayed in nitrogen-free Jensen's growth media, as presented in Figure 4. Among the root endophytes, the strains Klebsiella sp. HSTU-Bk11, Acinetobacter sp. HSTu-ABk29, Enterobacter sp. HSTu-ABk36, Serratia marcescens HSTu-ABk41 were grown well in Jensen's media, which was further evidenced by the creation of a holo zone of 11-15.8 mm in diameter. However, the expansion of the holo zone was limited to 5.5-7.75 mm in Enterobacter sp. HSTu-ABk38 and Citrobacter sp. HSTu-ABk30.  The nitrogen fixation activities of the pesticide-degrading endophytic bacteria were assayed in nitrogen-free Jensen's growth media, as presented in Figure 4. Among the root endophytes, the strains Klebsiella sp. HSTU-Bk11, Acinetobacter sp. HSTu-ABk29, Enterobacter sp. HSTu-ABk36, Serratia marcescens HSTu-ABk41 were grown well in Jensen's media, which was further evidenced by the creation of a holo zone of 11-15.8 mm in diameter. However, the expansion of the holo zone was limited to 5.5-7.75 mm in Enterobacter sp. HSTu-ABk38 and Citrobacter sp. HSTu-ABk30.

Anti-Bacterial Activity against Multidrug-Resistant Human Pathogenic Bacteria
The growth inhibition activity of the endophytic bacterial isolates against four multidrug-resistant human pathogenic bacteria was observed (Table 2; Supplementary Figure  S2). The results revealed that 40% of the endophytic isolates possessed anti-bacterial activities against S. aureus after 16 h of treatment, which rose to 55% and 65% after 32 h and 48 h, respectively. The highest inhibition zones of 41.5 ± 1.5 and 26 ± 0.6 mm were created by Enterobacter cloacae HSTu-ABk39 and Acinetobacter sp. HSTu-ABk34, respectively, against S. aureus after 32 h of treatment. It is noteworthy that 35% of the endophytic isolates produced inhibition zones against E. coli within 16 h of treatment, which increased to 55% after 32 h; however, these were less effective compared to those treatments observed against S. aureus. A similarly poor activity was demonstrated by these isolates when they were tested against Klebsiella sp. The second highest inhibition zone of 29 ± 0.9

Effects of Individual and Consortia of Endophytes on Germination and Seedling Growth
The germination of Shunamukhi genotype rice seeds was tested after the inoculation of bacterial endophytes individually and consortia of endophytes (Table 3). There was a significant difference among the treated and control samples after 8 days. The root and shoot lengths were significantly increased after 8 and 12 days of treatment of individual endophytes compared to those treated in the control (no endophytes). The LSD value of seed germination (9.55%), shoot lengths after 8 days (1.40), shoot lengths after 12 days (2.02), root lengths after 8 days (2.73), root lengths after 12 days (2.78), and the vigor index (354.61) were recorded for plants treated with individual endophytes (Table 3; Supplementary Figure S2). The germination percentages were not significantly different when treated with any consortia of endophytes. However, consortium-1 and consortium-2 treatments displayed the highest shoot growth compared with those treated with either no bacterial endophytes (control) or consortium-3 and consortium-4. In addition, the most increased vigor activity was observed for consortium-1 treated samples (Table 3; Supplementary Figure S2). Treatments with the same letter are not significantly different.

Effects of Consortia on Vegetative and Reproductive Stages and Yield
The effects of all four endophytic consortia on vegetative growth, reproductive growth, and yield of rice plants are presented in Figure 6.

Chlorophyll Content
Rice plants treated with compost mixed with consortium-2 produced the largest amount of chlorophyll a. On the other hand, the highest levels of chlorophyll b and total chlorophyll contents were found in the fertilizer treated rice plants (control). However, the highest ratio of chlorophyll a and chlorophyll b was observed in the com + consortium-2 treated rice plants ( Figure 6A). Although the urea application was reduced by 70% in consortia (1-4) treated rice plants, there were no significant differences in chlorophyll ratio between the consortia treated plants and fertilizer treated (control) plants. This result indicates that the bacterial consortia amended nitrogen from the atmosphere and/or fortified the nutrients from the added compost.

Root and Shoot Lengths
All four consortia treated rice plants demonstrated significant shoot growth compared to those of the control and fertilizer treated plants. Consortium-3 produced maximum shoot growth activity ( Figure 6B) compared to those plants treated with other consortia-1, -2, and -4. When compost was added, no significant shoot elongation of rice plants was observed among all four bacterial consortia treated plants.
There was a significant difference in rice plant root length among consortia treated plants ( Figure 6C). Again, consortia-3 produced maximum root growth compared with other consortia (-1, -2, and -4) treated rice plants, as among those observed for shoots. It is important to note that both compost and consortia (1-4) treated rice plants were much more greenish and disease free compared to the control, fertilizer, and compost + fertilizer treated plants ( Figure 6D(a-d)).

Chlorophyll Content
Rice plants treated with compost mixed with consortium-2 produced the largest amount of chlorophyll a. On the other hand, the highest levels of chlorophyll b and total chlorophyll contents were found in the fertilizer treated rice plants (control). However, the highest ratio of chlorophyll a and chlorophyll b was observed in the com + consortium-2 treated rice plants ( Figure 6A). Although the urea application was reduced by 70% in consortia (1-4) treated rice plants, there were no significant differences in chlorophyll ratio between the consortia treated plants and fertilizer treated (control) plants. This result indicates that the bacterial consortia amended nitrogen from the atmosphere and/or fortified the nutrients from the added compost.

Root and Shoot Lengths
All four consortia treated rice plants demonstrated significant shoot growth compared to those of the control and fertilizer treated plants. Consortium-3 produced maximum shoot growth activity ( Figure 6B) compared to those plants treated with other consortia-1, -2, and -4. When compost was added, no significant shoot elongation of rice plants was observed among all four bacterial consortia treated plants.
There was a significant difference in rice plant root length among consortia treated plants ( Figure 6C). Again, consortia-3 produced maximum root growth compared with other consortia (-1, -2, and -4) treated rice plants, as among those observed for shoots. It is important to note that both compost and consortia (1-4) treated rice plants were much more greenish and disease free compared to the control, fertilizer, and compost + fertilizer treated plants ( Figure 6D(a-d)).

Yield
Consortium-2 treated rice plants produced maximum dry weight at harvest ( Figure 6E). Notably, fertilizer, compost + Fertilizer, consortium-1, consortium-2, consortium-3, and consortium-4 treated rice plants showed similar yields (1000 seeds) compared with that of the control plants ( Figure 6F). In contrast, the grain weights of 10 tillers/crops were significantly higher for the compost mixed with consortium-2 treated rice plants harvested from pot experiments. In addition, pots containing compost mixed with either consortium-1, consortium-2, consortium-3, or consortium-4 increased the rice yields by 17.3%, 38.6%, 18.2%, and 39.1%, respectively, compared to those pots containing only compost mixed with fertilizer (urea).

Discussion
Previously, organochlorine, organophosphorus, and carbamate group pesticides were widely used in agricultural fields in Bangladesh [34]. However, due to the banning of organochlorine group pesticides by the Bangladesh Environment Conservation Act 1995 [35], organophosphorus pesticides are widely used in agriculture. Chlorpyrifos (O,O-diethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate) is one such widely used organophosphate pesticide employed to control a range of insects and pests in agriculture. The application of chlorpyrifos to increase crop yield poses a health risk to humans, animals, and other organisms alike. At high levels of chlorpyrifos exposure, this inherent neurotoxin can be lethal to humans [36]. To mitigate this issue, organophosphorus pesticide-degrading endophytic bacteria are much more desirable for biofertilizer components to develop a safe and sustainable agriculture practice. Endophytes exhibit cell wall hydrolytic and lignin-related dye-degrading enzyme activities, facilitating their penetration into host plants as symbionts. Additionally, their anti-microbial activities enable them to protect themselves and resist pathogenic strains during symbiosis, thus promoting healthier plants [20,21,37,38]. Furthermore, pesticide-degrading endophytes can contribute to the remediation of soil and plants by alleviating pesticide contamination [7,37,39]. These combined properties enable endophytes to fulfill their endophytic roles in host plants, fostering the development of resilient plants, which are resistant to pests and pathogens [20,40,41]. The current study demonstrated that the application of this synthetic consortium for rice cultivation can lead to healthy and high-yielding plants without the need for pesticides. This approach holds promise for promoting safe and sustainable agriculture practices.
In this paper, we report a total of eighteen endophytic bacterial strains isolated from Kalijeera (Field-1) and BRRI-28 (Field-2) genotypic rice plants capable of mineralizing chlorpyrifos. Employing a culture-dependent technique, these endophytic bacterial strains were grown and reproduced with chlorpyrifos serving as the only source of carbon, demonstrating a strong pesticide-degrading capability [7,8,15]. Furthermore, these strains were evaluated for their roles in the growth promotion of Shuna6+mukhi rice plant along with their inhibitory activities toward multidrug-resistant human pathogenic bacteria.
The 16S rRNA gene sequencing analysis of these strains indicated that the eighteen isolates belonged to six different species, including Klebsiella sp., Acinetobacter sp.,

Discussion
Previously, organochlorine, organophosphorus, and carbamate group pesticides were widely used in agricultural fields in Bangladesh [34]. However, due to the banning of organochlorine group pesticides by the Bangladesh Environment Conservation Act 1995 [35], organophosphorus pesticides are widely used in agriculture. Chlorpyrifos (O,Odiethyl O-3,5,6-trichloro-2-pyridyl phosphorothioate) is one such widely used organophosphate pesticide employed to control a range of insects and pests in agriculture. The application of chlorpyrifos to increase crop yield poses a health risk to humans, animals, and other organisms alike. At high levels of chlorpyrifos exposure, this inherent neurotoxin can be lethal to humans [36]. To mitigate this issue, organophosphorus pesticide-degrading endophytic bacteria are much more desirable for biofertilizer components to develop a safe and sustainable agriculture practice. Endophytes exhibit cell wall hydrolytic and lignin-related dye-degrading enzyme activities, facilitating their penetration into host plants as symbionts. Additionally, their anti-microbial activities enable them to protect themselves and resist pathogenic strains during symbiosis, thus promoting healthier plants [20,21,37,38]. Furthermore, pesticide-degrading endophytes can contribute to the remediation of soil and plants by alleviating pesticide contamination [7,37,39]. These combined properties enable endophytes to fulfill their endophytic roles in host plants, fostering the development of resilient plants, which are resistant to pests and pathogens [20,40,41]. The current study demonstrated that the application of this synthetic consortium for rice cultivation can lead to healthy and high-yielding plants without the need for pesticides. This approach holds promise for promoting safe and sustainable agriculture practices.
In this paper, we report a total of eighteen endophytic bacterial strains isolated from Kalijeera (Field-1) and BRRI-28 (Field-2) genotypic rice plants capable of mineralizing chlorpyrifos. Employing a culture-dependent technique, these endophytic bacterial strains were grown and reproduced with chlorpyrifos serving as the only source of carbon, demonstrating a strong pesticide-degrading capability [7,8,15]. Furthermore, these strains were evaluated for their roles in the growth promotion of Shuna6+mukhi rice plant along with their inhibitory activities toward multidrug-resistant human pathogenic bacteria.
The 16S rRNA gene sequencing analysis of these strains indicated that the eighteen isolates belonged to six different species, including Klebsiella sp., Acinetobacter sp., Pseudomonas sp., Citrobacter sp., Burkholderia sp., and Serratia sp. While a wide range of chlorpyrifos mineralizing strains were found in the root samples, Enterobacter sp. (50%) and Acinetobacter sp. (50%) were found to be the most dominant among the shoot and leaf endophytic strains, respectively. Previously, several chlorpyrifos degrading bacteria, such as the Enterobacter strain B14 [42] and the Klebsiella sp. [43], were reported. Like the present study, endophytic strains of the Enterobacter sp. were previously isolated from the roots and grains of rice plants by Walitang et al. (2017) [44]. Other chlorpyrifos degrading endophytes, such as the Pseudomonas aeruginosa strain RRA, Bacillus megaterium strain RRB, Sphingobacterium siyangensis strain RSA, Stenotrophomonas pavanii strain RSB, and Curtobacterium plantarum strain RSC, were also isolated from chlorpyrifos treated rice plants grown in China [45]. All of these eighteen strains along with four different consortia included the strains reported in this paper (Figure 2A-C), demonstrating rice plant growth-promoting traits (Table 3; Figure 6), including enhanced germination rate, and increasing the root-shoot length and yield in the presence of only 30% urea application (Table 3; Figure 6D-F). In fact, a substantial amount of rice yields was obtained with consortia treatments, mimicking the yields under full-dose urea fertilizer (100%) applications. In the present study, a synthetic consortium consisting of diverse bacterial genera was utilized to enhance the growth and development of rice plants, enabling them to withstand environmental stressors, such as drought, heat, and pathogenic infections [38][39][40][41]. This endophytic consortium demonstrates the capacity to support plants in tolerating adverse conditions and resisting pathogen attacks, thus promoting their overall resilience and health. All four consortia significantly enhanced rice grain yields in pot experiments ( Figure 6F) and degraded chlorpyrifos (Figure 7). In particular, the enhanced growth (4.3-6.5 log 10 CFU/mL) in chlorpyrifos enriched with minimal nutrient media can be attributed to the abilities of these strains utilizing chlorpyrifos as their sole carbon source, as described above in Section 3.4. While consortia-2, -3, and -4 showed several degraded fragments of chlorpyrifos, consortium-3 presented fragments containing phorate sulfoxide, phosphoric acid, and acetamide, and consortium-4 showed thionodemeton sulfone, phosphorodithioic acid, and thiophene, which suggested their varied action toward chlorpyrifos in culture media (Figure 7; Table 4; Supplementary Tables S1 and S2). This probably occurred due to their different crew members in consortia exerting various sets of enzyme activities and metabolic routes. The existence of common compounds, such as TCP (2-Hydroxy-3,5,6 trichloropyridine), DEMP (Diethyl methanephosphonate), ensured that the consortia members degraded the phosphodiester bonds of chlorpyrifos. Haque et al. (2018Haque et al. ( , 2020 reported that organophosphorus hydrolase enzyme OpdA, OpdC, OpdD, OpdE of Lactobacillus species isolated from chlorpyrifos impregnated fermented food, e.g., kimchi samples degraded chlorpyrifos ester bonds to non-toxic compounds TCP and DETP [8,16]. However, the generation of several other chlorpyrifos derivatives detected for the first time in this study was due to the efficiency of the GC-MS techniques along with the NIST11 library search, which opens a new window for chlorpyrifos detoxification by endophytic consortia.
Almost all of the endophytic strains reported in this paper showed oxidase, catalase, xylanase, amylase, protease, and cellulase activities, which are crucial for endophytic competence. Catalase activity defends reactive oxygen species and is essential for the successful survival of colonizing endophytes during oxidative bursts by plants [46]. Previously, similar activities were reported for catalase in the Klebsiella sp. strain PS19 [47], for protease in the Burkholderia sp., Pseudomonas sp., Enterobacter sp., Pseudomonas sp. [48,49], and for amylase in the Pseudomonas sp. [48]. In our study, we found that all but one endophyte, the Acinetobacter sp. strain HSTU-Bk12, secreted xylanase. However, several studies revealed the Acinetobacter sp. as a cellulase producer, and the Pseudomonas sp. and Enterobacter sp. as cellulase and xylanase producers [7,15].
The bioremediation of dye by endophytic bacteria would be non-hazardous, environmentally friendly, and cost effective. In this study, all strains except the Acinetobacter sp. HSTU-ABk29 and Acinetobacter sp. HSTU-ABk32 degraded different dyes, including TPB, CR, TDB, ATB, and BTB. The Acinetobacter sp. strains HSTU-ABk29 and HSTU-ABk32 were unable to degrade BTB and BTB dyes, respectively. As reported previously (Ali et al., 2009) [50], we also found that the Pseudomonas sp. could decolorize dye solution or simulated effluents. In addition, Tony et al. (2009) [51] reported that the consortia of microbes were capable of complete mineralization of azo dyes. However, endophytes harboring lignin-degrading activities might be presented with an opportunity to penetrate plants, forming symbionts [52]. An endophyte, which produces cellulase, protease, chitinase, and gelatinase, can hydrolyze fungal cell walls, and can inhibit the adhesion of fungal spores to plants, will have an advantage in mitigating fungal infection and be able to serve as a bio-fungicide.
The production of ammonia by endophytes is also a desirable trait for plant growth promotion, including the early establishment of seedlings, enhanced soil fertility, and increased phosphate solubilization [53]. Oteino et al. (2015) [54] observed that the inoculation with endophytes increased phosphate solubilization and significantly improved plant growth, which might be attributed to increased growth and development of rice plants treated with all four consortia. Auxin-producing endophytes Burkoholderia vietnamiensis promoted rice growth and yield [49]. Nitrogen-fixing bacterium Pantoea agglomerans [45] increased the growth of rice roots, shoots, flag leaves, and weights. Meanwhile, Lysinibacillus sphaericus produced ACC-deaminase and positively modulated the ethylene level in rice plants, consequently improving the number of panicles and grains per plant, straw, grain dry weight, and N and P uptake [55,56]. Moreover, additional Zinc (Zn) fortification was observed in rice treated with endophytes, such as the Acinetobacter sp., Klebsiella sp., Enterobacter sp., and Bukholderia sp. [57].
Bacterial consortia treatment of the Shunamukhi rice plant in a pot showed significant plant-growth-promoting effects in terms of root lengths, shoot lengths, yields, and chlorophyll contents compared with the control groups ( Figure 6D,F). Higher cell numbers (10 11 CFU/mL) of a growth-promoting endophytic bacterial consortium isolated from swamp soil have shown increased rice yield [65]. Previous research showed that nitrogenfixing endophytic bacteria treatment directly influenced grain yield parameters in rice plants [66,67]. In a similar study, Yanni and Dazzo (2010) [68] reported that rice grain yield was augmented by 41% when they used endophytic Rhizobium leguminosarum as the inoculant, which is in support of our findings of enhanced yields when rice plants were treated with consortium-2 and consortium-4 ( Figure 6F). Hence, the increased grain yield (38-39%), along with no changes in the total chlorophyll content even under the 70% reduced doses of nitrogen fertilizer, can be attributed to the cumulative effects of the plant-growth-promoting traits possessed by each of the crew members of all four consortia in this study. Several other studies reported similar findings, namely that plant-growth-promoting traits of endophytic bacteria augmented the nutrient uptake and enhanced rice yields [68][69][70] and yields of zucchini [71] and bermudagrass [72]. The endophytic strain of Lysinibacillus sphaericus [56] also demonstrated various aspects of plant-growth-promoting traits, such as those of the four consortia reported in the present study, which could accelerate plant growth along with fortifying the plant structure to provide resistance to phytopathogens. This finding might be indicative of the potential promises of these naturally occurring strains for sustainable rice production. Similarly, hormones such as auxin produced by endophytic consortia enhanced the root length and volume, augmented early seedling establishment, and increased nutrient intake from the soil ( Figure 6C). Rice plants treated with compost mixed with any of the four consortia resulted in more tillers and longer spikelets, which collectively contributed to the increased (38-39%) grain weights ( Figure 6F). The potential rice grain yield was affected by the increased rate of leaf photosynthesis, which could have an impact on dry matter production [73]. The fertilizer and consortium treatments showed a little difference in chlorophyll concentration, but the consortium treatments produced more rice. This might be attributed to other relevant factors, such as phytohormone synthesis and the furnishing of the crop structure toward immunity against biotic and abiotic stresses.
To date, there are no published reports on the effects of a consortium comprising naturally occurring endophytes on rice plants regarding the bioremediation of pesticides and urea reduction in field conditions. We assumed that the efficacy of consortia, comprising endophytic bacteria, as well as based biofertilizer harboring bioremediation properties, provided rice genotype Shonamukhi with resistance against both biotic and abiotic stresses in the field rather than in laboratory or greenhouse conditions ( Figure 6D). In turn, the effects of these consortia comprising different species of endophytic bacteria on rice plants resulted in increased plant growth and tiller number ( Figure 6D). This can be attributed to the abilities of members of the consortia to solubilize micronutrient and/or organic matter of the compost and make it available for root uptake. This was further confirmed by comparing the reduced growth and tillering performances of rice plants grown in compost mixed fertilizer only ( Figure 6D). The significant growth-promoting role of pesticide-degrading rice endophytes along with their consortia and subsequent yield is a novel finding, which can be applied to manage a safe and sustainable agricultural practice. Furthermore, the reduced amount of nitrogen fertilizer (30%) along with endophytic consortia as a biofertilizer in rice cultivation may lower the demand for chemical fertilizers, such as urea, which, in turn, might have a beneficiary economic impact on farming in Grameen Bangladesh.

Conclusions
The tendency of a quick gain is increasing, resulting in uncontrolled and improper application of pesticides and fertilizers to enhance crop yield. However, the residues from excessive applications of pesticides and fertilizers emerge with their persistence in the environment and are responsible for the death of many endophytic species, leading to agricultural disaster through minimizing plant survival, growth, and subsequent yield. We isolated and identified eighteen endophytic bacterial isolates with high activities of growthpromoting auxin (IAA), ACC-deaminase, N-fixation, P-solubilization, and lignocellulolytic enzymes. In addition, we developed four consortia of these endophytic bacteria, which led to the successful growth and yield of rice at lower doses of urea (30%). The anti-bacterial along with plant-growth-promoting properties of these naturally occurring endophytic bacteria created the potential routes for their use as pesticide degraders and growth promoters in agriculture and as sources of anti-bacterial drugs in pharmaceuticals. The application of endophytic bacteria in agriculture as a microbial inoculant will reduce fertilizer utilization, as well as agricultural health hazards. These research outputs combined with bio-stimulant technologies will help us develop the inoculants of naturally occurring pesticide-degrading bacterial endophytes to substitute synthetic chemical fertilizers, creating a safe and sustainable agricultural practice, as articulated in the Sustainable Development Goals (also known as Global Goals) adopted by the United Nations in 2015.
Supplementary Materials: The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/microorganisms11071821/s1: Figure S1: Phosphate solubilizing activities of the chlorpyrifos mineralizing endophytic bacteria; Figure S2 Figure S3: (a-d) Anti-bacterial activity of endophytic bacteria against multidrug-resistant human pathogenic bacteria S. aureus, E. coli, Klebsiella sp., S. epidermidis after 32 h. Table S1: Biodegradation profile of chlorpyrifos (1 gm/100 mL) in minimal broth medium after treatment with endophytic bacterial synthetic consortium-2. Table S2: Biodegradation profile of chlorpyrifos (1 gm/100 mL) in minimal broth medium after treatment with endophytic bacterial synthetic consortium-4. Informed Consent Statement: Informed consent was obtained from all subjects involved in the study.

Data Availability Statement:
The data reported in this study are contained within the article. The 16S rRNA gene sequences of the bacterial strains were deposited in NCBI and will be available immediately after acceptance of the manuscript for publication. The underlying raw data are available on request from the corresponding author.

Conflicts of Interest:
The authors declare no conflict of interest.