Biodegradation of Oil by a Newly Isolated Strain Acinetobacter junii WCO-9 and Its Comparative Pan-Genome Analysis

Waste oil pollution and the treatment of oily waste present a challenge, and the exploitation of microbial resources is a safe and efficient method to resolve these problems. Lipase-producing microorganisms can directly degrade waste oil and promote the degradation of oily waste and, therefore, have very significant research and application value. The isolation of efficient oil-degrading strains is of great practical significance in research into microbial remediation in oil-contaminated environments and for the enrichment of the microbial lipase resource library. In this study, Acinetobacter junii WCO-9, an efficient oil-degrading bacterium, was isolated from an oil-contaminated soil using olive oil as the sole carbon source, and its enzyme activity of ρ-nitrophenyl decanoate (ρ-NPD) decomposition was 3000 U/L. The WCO-9 strain could degrade a variety of edible oils, and its degradation capability was significantly better than that of the control strain, A junii ATCC 17908. Comparative pan-genome and lipid degradation pathway analyses indicated that A. junii isolated from the same environment shared a similar set of core genes and that the species accumulated more specific genes that facilitated resistance to environmental stresses under different environmental conditions. WCO-9 has accumulated a complete set of oil metabolism genes under a long-term oil-contamination environment, and the compact arrangement of abundant lipase and lipase chaperones has further strengthened the ability of the strain to survive in such environments. This is the main reason why WCO-9 is able to degrade oil significantly more effectively than ATCC 17908. In addition, WCO-9 possesses a specific lipase that is not found in homologous strains. In summary, A. junii WCO-9, with a complete triglyceride degradation pathway and the specific lipase gene, has great potential in environmental remediation and lipase for industry.


Introduction
Fumes, oily wastewater and oily solid waste generated by the catering industry have caused a series of environmental problems. Untreated oily wastewater enters drainage pipes easily, causing clogging, bad odors and other problems, which in turn, place a significant burden on urban sewage treatment, with solid oily waste further increasing treatment cost and degradation difficulty [1,2]. Traditional physicochemical methods (such as separation, landfill, flocculation, electrolysis, etc.) [3,4] for the treatment of waste oil are expensive and create secondary pollution problems [5]. One study showed that the degradation rate of hot pot waste oil by Klebsiella pneumoniae LZU10 was 37.7% in 48 h [6].  screened and constructed microbial flora for in situ biodegradation of waste

Isolation and Screening of Strains
The oil-contaminated soil sample was collected from the Southwest University of Science and Technology, Mianyang, Sichuan Province (N 31.53791, E 104.69388). For the isolation and screening of lipase-producing strains, 5 g of the soil sample was weighed in 45 mL of physiological saline, incubated for 1 h at 30 • C while being shaken at 200 r/min, and then rested. A quantity of 1 mL of supernatant was added to 20 mL of enrichment medium (yeast paste 0.2 g/L, NaCl 0.5 g/L, Na 2 HPO 4 3.5 g/L, KH 2 PO 4 1.5 g/L, MgSO 4 -7H 2 O 0.5 g/L), and this was incubated for 24 h at 30 • C while being shaken at 200 r/min. After gradient dilution of the enrichment medium, 100 µL of 10 −6~1 0 −8 dilution was coated onto the oil assimilation plate containing rhodamine B (25 mL olive oil emulsion was mixed well with 175 mL LB medium, 200 µL 10% rhodamine B solution was added, and the mixture was shaken). After incubation at 30 • C for 2 d, a pure culture capable of producing degradation circles was obtained, named WCO-9. The single colony of strain WCO-9 was selected and inoculated in an LB liquid medium. Cells in the logarithmic growth phase were obtained by shaking at 200 r/min and incubating at 30 • C for 12 h and were then stored in glycerol tubes. Finally, the WCO-9 strain was identified and registered by the Guangdong Microbial Culture Collection Center with the serial number GDMCC No: 61851. The scanning electron microscopy observation, 16S rDNA analysis and whole genome sequencing were performed using cells from the logarithmic growth stage.

Phylogenetic Analysis of 16S rDNA and Housekeeping Genes
The total genomic DNA of the WCO-9 strain was extracted using a commercial bacterial whole genome extraction kit (Tiangen, Beijing, China). The 16S rDNA sequence of the strain was amplified using universal primer F27/R1492, and the purified 16S rDNA fragments were sequenced by BGI (Beijing, China). Sequencing of the housekeeping genes (ileS, recA, rpoD) was carried out by BioMarker (Beijing, China). The NCBI BLAST system (https://www.ncbi.nlm.nih.gov/, accessed on 26 June 2022) was used to compare and analyze the 16S rDNA and the housekeeping genes. MEGA 11.0 software was used to construct a phylogenetic tree using the neighbor-joining method, and bootstrap values were calculated based on 1000 replicates. Phylogenetic analysis identified A. junii ATCC 17908 (ACCC preservation number: 11037) as the closest relative to strain WCO-9, and this was purchased from ACCC as a control for comparative analysis.

Comparison of the Growth and Oil Degradation Capabilities of Strain WCO-9
LB liquid medium was used to explore the optimal growth, lipase production temperature and pH of the WCO-9 strain, and ρ-nitrophenyl decanoate (C10), ρ-nitrophenyl laurate (C12), ρ-nitrophenyl myristate (C14), ρ-nitrophenyl palmitate (C16) and ρ-nitrophenyl stearate (C18) was chosen as the substrate for the lipase activity assay [34]. Oil degradation assays were performed on rhodamine B oil plates [35] using corn oil, soybean oil, peanut oil, canola oil and olive oil mixed with a solution of 4% polyvinyl alcohol (PVA) in a 1:3 volume ratio and emulsified by sonication for 10 min. Then, 25 mL of the emulsion was added to 200 mL of medium and stained with 10% rhodamine B solution. The WCO-9 and A. junii ATCC 17908 strains were activated by plate streaking, and single colonies were incubated in a liquid medium at 30 • C and shaken at 200 r/min for 12 h to prepare a seed solution, which was then inoculated in fresh liquid medium starting with an OD 600 of 0.1. When both strains reached an OD 600 of 0.7 ± 0.05, the bacterial concentrations of the two strains were adjusted to the same OD 600 value. The oil plate was punched, and 10 µL of the two bacterial solutions with the same OD 600 value was injected into the well and incubated at 30 • C for 4 days. The plate was observed under UV light, and the size of the transparent circle was measured as the parameter of oil degradation capabilities. The oil degradation activity was tested by GB/T_23535-2009 method with the five oils mentioned above.

Whole Genome Sequencing and Pan-Genome Analysis
The extraction and sequencing of the bacterial WCO-9 genome were performed by BioMarker in Beijing based on the Nanopore sequencing platform. The generated dataset corresponded to a sequencing depth of 100× The assembly of genomic data and correction of assembly results were performed using Canu v1.5 and Racon v3.4.3; gene prediction was performed using Prodigal v2.6.3; rRNA and tRNA were predicted using Infernal v1.1.3 and tRNAscan-SE v2.0 from non-redundant protein (NR); and Swiss-Prot, Pfam, Clusters of Orthologous Group (COG), Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to obtain functional annotation of WCO-9 genes.
To further clarify the taxonomic status of strain WCO-9 and understand its specificity, pan-genomic analysis was performed using the genomes of 11 different A. junii. Using a bacterial pan-genome analysis tool (BPGA) channel with a homology sequence threshold of 50%, the core genome of A. junii was calculated based on the established functional model, and a neighbor-joining phylogenetic tree between the strains was derived based on core homologous proteins. The average nucleotide identity between A. junii strains was calculated using the BLAST (ANIb) and MUMmer (ANIm) algorithms, and the DNA-DNA hybridization (DDH) results of WCO-9 with the other 10 strains were obtained via numerical simulation using the Genome-to-Genome Distance Calculator (GGDC 2.1) (http://ggdc.dsmz.de/ (accessed on 26 June 2022)).

Analysis of Lipase Genes and Triacylglycerol Degradation Pathway
To discover the high lipase activity and lipid degradation capability of WCO-9, several lipase, lipase chaperone and triacylglycerol metabolic pathway genes of WCO-9 were analyzed. The lipase and lipase chaperone genes of strain WCO-9 were obtained by BLAST comparison with databases such as NR, Swiss-Prot, COG, Pfam, etc. The triacylglycerol degradation pathway of WCO-9 was obtained by a BLAST comparison of the sequenced related protein sequences with the sequences included in the KEGG database to find the most similar sequences. The sequence annotation information, the corresponding KO number, and the position in the metabolic pathway corresponding to the KO also represent the annotation information for the corresponding genes in the sequenced genome.
According to the phylogenetic tree of the core genes, A. junii ATCC 17908, A. junii CAM121, A. junii lzhX15, A. junii NIPH182 and A. junii YR7 belonged to different branches of the evolutionary tree; therefore, these strains were selected for the whole genome comparison with strain WCO-9. Genome-wide comparisons were carried out using the BLAST Ring Image Generator (BRIG) and image generation was performed using CGView with gene BLAST parameter values of 1 × 10 −5 and gene identity thresholds of 70% (upper identity) and 50% (lower identity). Information in the triacylglycerol degradation pathway relating to lipase genes and other key enzyme genes of the WCO-9 strain were annotated in the outermost circle.

Statistical Analysis
The results were analyzed with GraphPad Prism 8.0.2, Origin2021 and SPSS Statistics 25 soft-mare. Differences in means between groups were compared for statistical significance at p < 0.05.

Screening and Identification of Strain WCO-9
The strain WCO-9 was isolated from an oil-contaminated soil, and the isolate produced a distinct degradation circle on agar plates containing olive oil supplemented with rhodamine B ( Figure 1A). Cultivation of this strain was improved in LB liquid medium at 30 • C, shaken at 200 r/min. Colonies of this strain were circular, convex, moist and opaque on the agar plates after 1 day of incubation at 30 • C. Staining experiments revealed that the isolate was Gram-negative ( Figure S1). The cell morphology was spherical or in the form of short rods, 0.6-1.0 µm long and 0.4-0.7 µm wide without flagella ( Figure 1B). The complete 16S rDNA sequence (1537 bp) of WCO-9 was used in the NCBI nucleotide database for comparative analysis and construction of the phylogenetic tree. The results showed that WCO-9 is an A. junii with 100% similarity to A. junii ATCC 17908 and less than 96% similarity to other genera of bacteria. The 16S rDNA phylogenetic analysis and partly housekeeping genes (ileS, recA, and rpoD) revealed that strain WCO-9 was clearly differentiated from other members of the A. junii family (Figures 2, S2-S4). The 16S rDNA phylogenetic analysis showed that WCO-9 clustered with A. junii and formed a monophyletic branch, which was related to A. junii ATCC 17908 ( Figure 2).

Statistical Analysis
The results were analyzed with GraphPad Prism 8.0.2, Origin2021 and SPSS Statistics 25 soft-mare. Differences in means between groups were compared for statistical significance at p < 0.05.

Screening and Identification of Strain WCO-9
The strain WCO-9 was isolated from an oil-contaminated soil, and the isolate produced a distinct degradation circle on agar plates containing olive oil supplemented with rhodamine B ( Figure 1A). Cultivation of this strain was improved in LB liquid medium at 30 °C, shaken at 200 r/min. Colonies of this strain were circular, convex, moist and opaque on the agar plates after 1 day of incubation at 30 °C. Staining experiments revealed that the isolate was Gram-negative ( Figure S1). The cell morphology was spherical or in the form of short rods, 0.6-1.0 μm long and 0.4-0.7 μm wide without flagella ( Figure 1B). The complete 16S rDNA sequence (1537 bp) of WCO-9 was used in the NCBI nucleotide database for comparative analysis and construction of the phylogenetic tree. The results showed that WCO-9 is an A. junii with 100% similarity to A. junii ATCC 17,908 and less than 96% similarity to other genera of bacteria. The 16S rDNA phylogenetic analysis and partly housekeeping genes (ileS, recA, and rpoD) revealed that strain WCO-9 was clearly differentiated from other members of the A. junii family ( Figure 2, Figures S2-S4). The 16S rDNA phylogenetic analysis showed that WCO-9 clustered with A. junii and formed a monophyletic branch, which was related to A. junii ATCC 17,908 ( Figure 2).

Growth Characteristics and Oil Degradation Capabilities of Strain WCO-9
The growth and enzyme production of the strains in LB medium were investigated under different temperature and pH conditions. In essence, the increase in biomass of the

Growth Characteristics and Oil Degradation Capabilities of Strain WCO-9
The growth and enzyme production of the strains in LB medium were investigated under different temperature and pH conditions. In essence, the increase in biomass of the strains was positively correlated with the increase in lipase activity. In LB medium with a pH of 7.0, strain WCO-9 was able to grow at 20, 25, 30, 35 and 40 • C, and 30 • C was the best temperature for growth and enzyme production temperature. When the temperature exceeded 30 • C, the growth and enzyme production ability of WCO-9 decreased sharply ( Figure 3A). The WCO-9 strain was able to grow in a pH range of 5.0-10.0 and could not grow at pH 4.0. Its optimal growth and enzyme production was at pH 6.0 ( Figure 3B). The lipase activity of WCO-9 was excellent, and the hydrolytic activity of ρ-NPD substrate produced by WCO-9 was 3040 U/L under optimal growth and enzyme production conditions.  The ability of WCO-9 and ATCC 17,908 to degrade various oils was analyzed us agar plates containing olive oil supplemented with rhodamine B, and the results show that strain WCO-9 had significant degradation effects on all five oils and was significan better than strain ATCC 17,908 (Table 1, Figure S5).
The degradation ability of strain WCO-9 was most evident for peanut oil, whereas ability to degrade olive oil was relatively weak. Among the five oils, the lowest degra tion activity for olive oil was 2833 U/L, and that for other oils was above 3000 U/L. T lipase activity of the WCO-9 strain was five times higher than that of the control str The results indicated that strain WCO-9 has potential application in the degradation waste cooking oil.  The ability of WCO-9 and ATCC 17908 to degrade various oils was analyzed using agar plates containing olive oil supplemented with rhodamine B, and the results showed that strain WCO-9 had significant degradation effects on all five oils and was significantly better than strain ATCC 17908 (Table 1, Figure S5).
Note: "ND" indicates that no oil degradation was observed. "−" indicates that the results had not been tested or could not be tested. The degradation ability of strain WCO-9 was most evident for peanut oil, whereas its ability to degrade olive oil was relatively weak. Among the five oils, the lowest degradation activity for olive oil was 2833 U/L, and that for other oils was above 3000 U/L. The lipase activity of the WCO-9 strain was five times higher than that of the control strain. The results indicated that strain WCO-9 has potential application in the degradation of waste cooking oil.

Genome Comparison and Pan-Genome Analysis of WCO-9
A. junii, as a major member of the genus Acinetobacter, is widely distributed in the natural world. Genomic data relating to 81 strains of A. junii have been published in the NCBI database, of which 10 were selected based on the comparison results of 16S rDNA sequences and complete genome sequencing of WCO-9. The 10 strains were selected in combination with other sources and genomic information in the database. Eleven strains of A. junii were isolated, mainly from the soil, lake, sewage and the human environment, with genome size ranging between 3.19 and 3.78 Mb and GC content between 38.6% and 39%. Of these, strain WCO-9 had the smallest genome size and number of genes ( Table 2). We calculated the ANIb and ANIm of the 11 A. junii genomes and the DNA hybridization values of WCO-9 with the genomes of the other 10 strains (Table 3). The ANIb value of strain WCO-9 with A. junii lzh-X15 was 98.18% (98.16%), and the ANIm values with the rest of the strains were above 97%, which is higher than the species cut-off threshold of 95-96%. The DDH values of strain WCO-9 with the remaining 10 strains were all above the DDH threshold for the delineation of new species (70%).  A comparative analysis was performed between strain WCO-9 and 10 strains of A. junii, setting a sequence similarity threshold of 50% and calculating the core genes of the A. junii genome ( Figure 4A). The genome of the 11 strains contained 35,215 genes, 2229 of which were core genes. The WCO-9 strain possessed 102 specific genes, accounting for 3.4% of the number of genes. KEGG annotation revealed that the specific genes were concentrated in metabolism and environmental information processing, primarily amino acid metabolism, carbohydrate metabolism, signal transduction, membrane transport, folding, sorting and degradation ( Figure S6). COG annotation showed a higher proportion of specific genes in transcription, replication, recombination and repair, and general function. Phylogenetic analysis of the core genes showed that the 11 strains of A. junii were divided into four sub-evolutionary branches ( Figure 4B), in which WCO-9 and A. junii lzh-X15 were both isolated from soil samples and clustered together in the evolutionary tree, whereas A. junii ATCC 17908 and A. junii CIP 107470, which were both isolated from liquid environments, were also grouped together and divided into different sub-branches because of the different compositions of the liquid environments.

Comparison of Lipase Genes and Triacylglycerol Metabolic Pathways
In order to further clarify the efficient lipid degradation mechanism of WCO-9, we used genome annotation to obtain all the lipases and chaperones in the WCO-9 genome. This showed that 11 lipase genes and 3 lipase chaperone genes were dispersed on the genome. The lipase chaperone lif1700 and lipases lip1701 and lip1702, and the lipase chaperones lif2659 and lipase lip2660 were separately arranged without spacers to form a gene cluster ( Figure 5A). The degradation of triacylglycerol was initiated by triacylglycerol lipase (TGL). In one of the degradation pathways, triacylglycerol was hydrolyzed by TGL to 1,2-diacylglycerol after the third ester bond and then transformed into 1,2-diacylglycerol-3-phosphate by diacylglycerol kinase (dgkA) to enter the oxidative metabolism of phosphoglycerate. In another pathway, triacylglycerol was degraded by TGL to monoacylglycerol after the third and first ester bonds were hydrolyzed. The other degradation pathway was the stepwise degradation of triacylglycerol into monoacylglycerol by TGL with ester bonds at positions 3 and 1, further degradation by monoacylglycerol lipase (MGLL), and then from glycerol into 1,2-diacylglycerol-3-phosphate after three steps of activation into a phosphoglycerol metabolism ( Figure 5B).

Acinetobacter junii CIP 107470
Acinetobacter junii YR7  Five strains from each branch of the core evolutionary tree were selected for comparison with WCO-9 for lipase, chaperone and triacylglycerol degradation pathway genes. The results showed that 10 lipase and 3 lipase chaperone genes could be found in other strains of the A. junii family; ATCC 17,908 lacked one lipase and one lipase chaperone compared with WCO-9; the sequence identity of two lipases and one lipase chaperone was less than 50% compared with WCO-9, and the two key initiator genes of the triacylglycerol degradation pathway (TGL2) were missing in all strains with the exception of strain NIPH 182 ( Figure 6). Strain WCO-9 had one specific lipase gene (lip1931). Lip1931 was located on a gene island in the genome of strain WCO-9, and one gene each to the left (GE_1930) and right (GE_1932) of lip1931 had no corresponding gene in the homologous strain. The rest of the genes on the gene island could be found in the comparison strains (Figure 7). Five strains from each branch of the core evolutionary tree were selected for comparison with WCO-9 for lipase, chaperone and triacylglycerol degradation pathway genes. The results showed that 10 lipase and 3 lipase chaperone genes could be found in other strains of the A. junii family; ATCC 17908 lacked one lipase and one lipase chaperone compared with WCO-9; the sequence identity of two lipases and one lipase chaperone was less than 50% compared with WCO-9, and the two key initiator genes of the triacylglycerol degradation pathway (TGL2) were missing in all strains with the exception of strain NIPH 182 ( Figure 6). Strain WCO-9 had one specific lipase gene (lip1931). Lip1931 was located on a gene island in the genome of strain WCO-9, and one gene each to the left (GE_1930) and right (GE_1932) of lip1931 had no corresponding gene in the homologous strain. The rest of the genes on the gene island could be found in the comparison strains (Figure 7).

Discussion
Acinetobacter sp. strains live in diverse environments and, when isolated from their Figure 6. Comparison of lipase, chaperone and triacylglycerol degradation pathway genes between five strains of WCO-9 and other A. junii. The outermost circle is the annotation of lipase, lipase chaperone and triacylglycerol degradation pathway genes. The blue box indicates that the gene is present in all 11 strains, and the grey box indicates consistency of this gene in the ATCC 17908 genome below 50% or not present compared with the WCO-9 strain, and the red box shows the specific lipase gene for strain WCO-9.
Microorganisms 2023, 11, x FOR PEER REVIEW 11 of 16 Figure 6. Comparison of lipase, chaperone and triacylglycerol degradation pathway genes between five strains of WCO-9 and other A. junii. The outermost circle is the annotation of lipase, lipase chaperone and triacylglycerol degradation pathway genes. The blue box indicates that the gene is present in all 11 strains, and the grey box indicates consistency of this gene in the ATCC 17,908 genome below 50% or not present compared with the WCO-9 strain, and the red box shows the specific lipase gene for strain WCO-9.

Discussion
Acinetobacter sp. strains live in diverse environments and, when isolated from their specific environments, have a significant lipid degradation capability and are, therefore,

Discussion
Acinetobacter sp. strains live in diverse environments and, when isolated from their specific environments, have a significant lipid degradation capability and are, therefore, valuable in environmental bioremediation [36,37]. Our study used SEM to examine the cell morphology of A. junii, which revealed two morphologies, short rod-shaped and spherical, with interconnected cells, consistent with the results of the study carried out by Nandi et al. (2020) [38]. A. junii, as a branch of the large family of the Actinobacter genus, has been reported primarily as a pathogenic bacterium [39,40]. In an early study of A. junii for environmental remediation, Hrenovic et al. (2011) immobilized the A. junii DSM 1532 strain on natural zeolitic tuff to remove phosphorus from municipal wastewater [41]. Since then, there has been a gradual increase in the number of studies concerning the use of A. junii for environmental waste removal, including in the treatment of nitrogenous wastewater [42] and the degradation of phenol [43]. Recent studies also confirmed that bioremediation techniques using A. junii in combination with plants could improve waste degradation rates [44,45].
In this study, we found that the lipase genes of A. junii are abundant in quantity and variety, but there are few reports concerning the use of A. junii for oil degradation. The strains WCO-9 and ATCC 17908 have the closest 16S rDNA sequence identity, but they display significant differences in their oil degradation abilities (Table 1, Figure S5). A comparison of lipase and lipase chaperone genes revealed that the ATCC 17908 strain lacked one secretory lipase and one secretory lipase chaperone compared with WCO-9. In addition, the sequence consistency of two lipases (TGL2) and two lipase chaperones of ATCC 17908 was less than 70% compared with WCO-9, resulting in insufficient extracellular lipase activity in the ATCC 17908 strain. We compared the genes in the WCO-9 triacylglycerol degradation pathway with five other strains, including ATCC 17908. The results showed that four strains, including ATCC 17908, lacked the TGL2 lipase, which is the initiator for triacylglycerol degradation, and could not directly utilize triacylglycerol; thus, ATCC 17908 was not effective in lipid degradation. We speculated that most of the strains in the A. junii family were not capable of oil degradation, which is the main reason why there are few studies on lipid degradation in A. junii, and this suggests that WCO-9 is a special strain in this family by virtue of its oil degradation capabilities.
Lipase chaperones play an important role in the formation of lipase structures and the improvement of lipase activity. The lipase chaperones in the WCO-9 strain are closely aligned with multiple lipases, suggesting that such an arrangement is conducive to the more efficient formation of the advanced lipase structure of strain WCO-9, which exhibits high lipase activity. The chaperones also assist in the folding and secretion of lipase, further improving the oil degradation capability. Studies by Zheng et al. (2012) and others support this view [46,47]. Pan-genome analysis revealed that the strains from the same types of environment share a similar set of core genes and are clustered in the same evolutionary branch. The species has an extremely strong environmental response mechanism to cope with different environmental stresses, which explains the wide distribution of A. junii in different natural environments. The strain genome has a high proportion of genes specific to environmental information processing and metabolic functions, and the genome has a high degree of environmental plasticity. The presence of waste oil increased the expression of the lipase gene in strain WCO-9, which demonstrated higher lipase activity than the homologous strain ATCC 17908. To further improve the degradation and utilization of oils, the bacterial genome underwent recombination, mutation or gene exchange between different bacterial species. Therefore, we suggest that the significant oil degradation capabilities of WCO-9 are attributable to the long-term environmental stress caused by oil pollution and are a result of the adaptive evolution of bacteria under environmental stress. The same conclusion has been reached by the authors of previous studies [48][49][50]. The widespread use of lipases in a variety of fields has led to the expansion of their market, and the demand for more active and environmentally friendly lipases, and specific lipases, has been increasing [51]. Acinetobacter sp. has been intensively studied as an important source of alkaline and low-temperature lipases [52][53][54]. During its long-term evolution, strain WCO-9 produced a specific lipase gene, which belongs to a novel lipase, the biological function and physicochemical properties of which have never been studied and have a high mining value.

Conclusions
In this study, we isolated a bacterial strain with significant oil degradation capabilities from an environment contaminated with waste cooking oils. We identified it as A. junii using 16S rDNA and genome sequencing and named it A. junii WCO-9. We investigated the growth conditions for strain WCO-9 and found that its growth characteristics and lipase activity were optimal at 30 • C and pH 6.0. The hydrolysis activity of the lipase produced by the WCO-9 to the ρ-NPD substrate was about 3000 U/L. In order to investigate whether A. junii members were generally capable of lipid degradation, A. junii ATCC 17908 and WCO-9 were used in degradation tests on four edible vegetable oils, and it was found that WCO-9 had significant degradation capabilities for all four oils, whereas ATCC 17908 had almost no degradation effects. In summary, the long-term oil pollution environment was the external driving force for the formation of high oil degradation capacity of strain WCO-9. Under the pressure of oil, a large number of favorable mutations occurred in the genome of WCO-9, and a complete triglyceride degradation pathway was formed. The lipase gene structure of WCO-9 was different from that of the same species strain, so the oil degradation performance of WCO-9 was significantly better than that of the control strain. Lip1931, a unique secreted lipase of WCO-9, was inserted into the genome of WCO-9, and in addition, the close arrangement of the lipase chaperone and lipase on the genome differed from that of the same species strain, resulting in significantly higher lipase activity than that of the control strain. The degradation ability and high lipase activity of WCO-9 indicate its research value in environmental remediation and lipase gene mining. In addition, a total of 10 lipases of 4 types were found in the other strains of the family, which are rich in lipase genes and are an important source of microbial lipases. This study revealed the genetic factors for the efficient oil degradation capabilities of the WCO-9 strain, further confirmed the environment-driven evolution of A. junii and demonstrated the utilization value of strain WCO-9 in the preparation of mixed bacterial agents for waste oil degradation and environmental bioremediation. Furthermore, it laid a foundation for microbial lipase gene mining and strain modification based on the triacylglycerol pathway.
Supplementary Materials: The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/microorganisms11020407/s1, Figure S1: Colony morphology and Gram staining of strain WCO-9. Figure S2: Phylogenetic tree based on the ileS gene sequence. This tree was constructed by the neighbor-joining method and showed the phylogenetic relationship between strain WCO-9 and closely related species. Figure S3: Phylogenetic tree based on the recA gene sequence. This tree was constructed by the neighbor-joining method and showed the phylogenetic relationship between strain WCO-9 and closely related species. Figure S4: Phylogenetic tree based on the rpoD gene sequence. This tree was constructed by the neighbor-joining method and showed the phylogenetic relationship between strain WCO-9 and closely related species. Figure S5: Degradation of five oils by strain WCO-9 and ATCC 17908: (A) corn oil, (B) peanut oil, (C) canola oil, (D) soybean oil; (E) olive oil. Figure S6