UME6 Is Involved in the Suppression of Basal Transcription of ABC Transporters and Drug Resistance in the ρ+ Cells of Saccharomyces cerevisiae

In Saccharomyces cerevisiae, the Rpd3L complex contains a histone deacetylase, Rpd3, and the DNA binding proteins, Ume6 and Ash1, and acts as a transcriptional repressor or activator. We previously showed that RPD3 and UME6 are required for the activation of PDR5, which encodes a major efflux pump, and pleiotropic drug resistance (PDR) in ρ0/− cells, which lack mitochondrial DNA. However, there are inconsistent reports regarding whether RPD3 and UME6 are required for Pdr5-mediated PDR in ρ+ cells with mitochondrial DNA. Since PDR5 expression or PDR in the ρ+ cells of the rpd3Δ and ume6Δ mutants have primarily been examined using fermentable media, mixed cultures of ρ+ and ρ0/− cells could be used. Therefore, we examined whether RPD3 and UME6 are required for basal and drug-induced PDR5 transcription and PDR in ρ+ cells using fermentable and nonfermentable media. UME6 suppresses the basal transcription levels of the ABC transporters, including PDR5, and drug resistance in ρ+ cells independent of the carbon source used in the growth medium. In contrast, RPD3 is required for drug resistance but did not interfere with the basal PDR5 mRNA levels. UME6 is also required for the cycloheximide-induced transcription of PDR5 in nonfermentable media but not in fermentable media.

Gain-of-function mutations, such as pdr1-3 and pdr3-7 in Pdr1 and Pdr3, lead to the overexpression of PDR3, PDR5, SNQ2, YOR1, PDR10, and PDR15, and thereby induce the PDR [6,12,[14][15][16]. This suggests that the exogenous compounds and drugs lead to the release of Pdr1 and Pdr3 from certain negative regulators because Pdr1 and Pdr3 directly bind a variety of exogenous compounds and drugs [17]. On the other hand, cycloheximide, In contrast, we also show that RPD3 is required for drug resistance but did not interfere with the basal PDR5 mRNA levels.

Spot Dilution Assay
The relative resistance of each yeast strain to fluconazole or cycloheximide was estimated with a spot dilution assay using YPG or YPD media [40,41]. ρ + cells from each yeast strain were aerobically grown to the logarithmic phase (an OD 600 of 0.6-0.9) at 30 • C in YPG or YPD media in triplicate. Five microliters of 10-fold serial dilutions of the YPG cultures containing the same number of cells was spotted on YPG plates with or without 3 µg/mL fluconazole (Nacalai Tesque) (or 0.1 µg/mL cycloheximide (Wako)) and incubated at 30 • C for 21 days. Five microliters of 10-fold serial dilutions of the YPD cultures containing the same number of cells was spotted on YPD plates with or not without 30 µg/mL fluconazole (Nacalai Tesque) (or 0.5 µg/mL cycloheximide (Wako)) and incubated at 30 • C for 21 days.

Cocultivation of Two Gene Deletion Mutants Replaced with KanMX or bleMX6 Gene Cassettes
One gene deletion mutant replaced with the KanMX gene cassette was cocultivated with the other gene deletion mutant that had been replaced with the bleMX6 gene cassette [32]. ρ + cells from each mutant corresponding to 0.1 OD 600 initial concentration were cocultivated in 10 mL of YPG or YPD media with or without 30 µg/mL fluconazole (or 0.5 µg/mL cycloheximide) [32]. The aliquots of the coculture were recovered immediately before drugs addition (at 0 min) and at various times after drug addition and spread on the YPD plates containing G418 or Zeocin [32,42]. The viability of each strain at each time point was estimated from the colony numbers on the G418 and Zeocin plates [32,42].
2.4. RNA Extraction from the ρ + Cells in Each Strain Grown to the Logarithmic Phase ρ + cells from each yeast strain were grown to the logarithmic phase (an OD 600 of 7-9) in YPG or YPD media in duplicate. The cultures were diluted to an OD 600 of 0.1 and grown to an OD 600 of 0.4-0.8 [32,43,44]. Aliquots of the cultures were recovered. The cells in the aliquots above were pelleted, washed, frozen at -80 • C, and used to extract total RNA [32,43,44]. Total RNA was isolated from the yeast cells using a NucleoSpin RNA kit (TaKaRa) according to the manufacturer's protocol.
2.5. RNA Extraction from ρ + Cells before and after Cycloheximide Exposure ρ + cells from the wild-type and ume6∆::bleMX6 strains were grown to an OD 600 of 7-9 in YPG or YPD media, diluted to an OD 600 of 0.1, and grown to an OD 600 of 0.4-0.8 in duplicate [32,43,44]. Aliquots of each duplicate were harvested just prior to the addition of cycloheximide to the medium. The duplicates were grown for 45 min or 90 min at 30 • C after exposure to cycloheximide (0.2 µg/mL). Aliquots of each duplicate were recovered at 45 min or 90 min after the addition of cycloheximide. The cells in the aliquots above were pelleted, washed, frozen at -80 • C, and used to extract total RNA [32,43,44]. Total RNA was isolated from the yeast cells before and after exposure to cycloheximide using a NucleoSpin RNA Plus kit (TaKaRa) according to the manufacturer's protocol.

Real-Time RT-PCR
Reverse transcription of total RNA was performed using the PrimeScript 1st strand cDNA Synthesis Kit (TaKaRa). SYBR Green qRT-PCR for cDNA from the individual duplicate samples was performed using the TB Green ® Premix Ex Taq II (TaKaRa) in a Step One Real-time PCR system (Applied Biosystems). A minus reverse transcriptase control was used as the negative control. Serial dilutions of control cDNA were prepared to produce a standard curve for each primer pair. The primers used for qRT-PCR are listed in Table S1. The transcription levels of each target gene were measured by qRT-PCR and normalized to ACT1, which was used as an endogenous control. The normalized levels are shown relative to samples from the wild-type or cycloheximide-untreated wild-type strains, which were set to 1.

Statistical Analysis
The survival rate of the ume6∆ strain at each time point in Figure 2 was normalized to that at 0 h. A paired t-test was used for statistical analysis in Figure 2. An unpaired Student's t-test was used for statistical analysis in Figures 3 and 4. Statistical significance was indicated as p < 0.05.

UME6 Acts as a Negative Regulator to Fluconazole-and Cycloheximide-Resistance in ρ + Cells, While RPD3 Acts as a Positive Regulator
To examine whether RPD3 and UME6 are responsible for PDR in ρ + cells of S. cerevisiae, a spot dilution assay using YPG medium containing the nonfermentable carbon source glycerol was carried out to determine the resistance or susceptibility in the ρ + cells of the rpd3∆ and ume6∆ mutants to the PDR substrates fluconazole and cycloheximide. ρ + cells of the wild-type strain required a lower concentration of fluconazole and cycloheximide for growth inhibition in YPG medium than in YPD medium, which is consistent with previous reports [45]. The ρ + cells of the ume6∆::bleMX6 mutant were slightly more tolerant to fluconazole and cycloheximide than those in the wild-type, ash1∆::bleMX6, pdr3∆::bleMX6, and gat3∆::bleMX6 strains ( Figure 1). However, the ρ + cells of the rpd3∆ mutant were more susceptible to fluconazole and cycloheximide than those in the wild-type, ume6∆::bleMX6, ash1∆::bleMX6, pdr3∆::bleMX6, and gat3∆::bleMX6 strains ( Figure 1).
The oligomycin susceptibility of the yeasts was tested on nonfermentable media like YPG medium [3,46]. However, Jensen et al. reported impaired endoplasmic reticulum (ER) to Golgi trafficking of Pdr5 in the rpd3Δ strain of ρ + S. cerevisiae in nonfermentable medium with glycerol and ethanol as the carbon sources but not in YPD medium [45]. In Figure 1. The ρ + cells of the ume6∆ mutant are more tolerant to fluconazole and cycloheximide than those of the wild type on YPG plates. Fluconazole and cycloheximide resistance of ρ + cells of the wildtype strain (FY1679-28C) and its derivative strains, ume6∆::bleMX6, ash1∆::bleMX6, pdr3∆::bleMX6, and rpd3∆::bleMX6, and gat3∆::bleMX6, were determined by spot dilution assay in YPG plates with or without 3 µg/mL fluconazole (or 0.1 µg/mL cycloheximide).
The oligomycin susceptibility of the yeasts was tested on nonfermentable media like YPG medium [3,46]. However, Jensen et al. reported impaired endoplasmic reticulum (ER) to Golgi trafficking of Pdr5 in the rpd3∆ strain of ρ + S. cerevisiae in nonfermentable medium with glycerol and ethanol as the carbon sources but not in YPD medium [45]. In addition, the PDR5 mRNA levels are downregulated in the ρ + cells of S. cerevisiae limited for glucose [8].
These reports indicate that the expression of the regulatory machinery of PDR5 in ρ + cells could be different between YPG and YPD cultures. Therefore, to examine whether RPD3 and UME6 are involved in PDR in ρ + cells cultured in YPD medium in the same way as that in YPG medium, a similar spot dilution assay as that described above was conducted in YPD plates with or without 30 µg/mL fluconazole (or 0.5 µg/mL cycloheximide). As a result, there were no significant differences in fluconazole and cycloheximide resistance between the wild-type, ume6∆::bleMX6, ash1∆::bleMX6, pdr3∆::bleMX6, and gat3∆::bleMX6 strains in YPD plates ( Figure S1). However, significantly higher susceptibilities to fluconazole and cycloheximide were found in the rpd3∆ mutant than those found in the wild-type strain ( Figure S1). The cycloheximide insusceptibility found in the ρ + cells of the ume6∆ and ash1∆ mutants in YPD medium was consistent with reports by Borecka-Melkusova et al. [33]. On the other hand, fluconazole insusceptibility, but not cycloheximide insusceptibility, in the ρ + cells of the ume6∆ mutant in YPD was consistent with reports by Yibmantasiri et al. [35].
In the spot dilution assay above, the ρ + cells of the ume6∆ mutant displayed slightly more tolerance to fluconazole and cycloheximide in YPG plates than the ρ + cells of the wild-type, pdr3∆, ash1∆, and gat3∆ strains ( Figure 1). To detect a slight difference in drug resistance between two mutant strains, we previously used a cocultivation assay [32]. Therefore, we further investigated whether the ρ + cells of the ume6∆ mutant confer fluconazole and cycloheximide tolerance in a cocultivation assay using YPG medium. The ash1∆ and gat3∆ mutants displayed an equal tolerance to fluconazole and cycloheximide compared to the wild-type strain in the spot dilution assay (Figure 1), and thereby, the ash1∆ and gat3∆ mutants were used as controls for the ume6∆ mutant in the cocultivation assay. ρ + cells of two mutant strains, ash1∆::kanMX and ume6∆::bleMX6, were cocultivated in the presence and absence of 0.5 µg/mL cycloheximide in YPG medium. The number of viable cells in each mutant strain in the coculture was estimated by spreading the cells on YPD plates containing G418 or Zeocin. We found that the ρ + cells of the ume6∆::bleMX6 strain were eliminated from the coculture over time in YPG medium without cycloheximide but accounted for a large percentage of the viable cells in the coculture over time in YPG medium containing 0.5 µg/mL cycloheximide (p < 0.05) (Figure 2A). Similarly, the ρ + cells of the ume6∆::KanMX strain were eliminated from the cocultures of the ume6∆::KanMX and ash1∆::bleMX6 strains in YPG medium over time in the absence of cycloheximide but selected in the presence of 0.5 µg/mL cycloheximide (data not shown). In addition, the ρ + cells of the ume6∆::KanMX strain were eliminated from the coculture over time in the absence of fluconazole but not in the presence of fluconazole when cocultivated with gat3∆::bleMX6 in YPG medium with or without 30 µg/mL fluconazole ( Figure 2B). Furthermore, similar results were observed for the ρ + cells of the gat3∆::KanMX and ume6∆::bleMX6 mutants cocultivated in YPG medium with or without 30 µg/mL fluconazole (data not shown).
We next examined whether the higher cycloheximide and fluconazole resistance in ume6∆ is dependent on the carbon source used in the growth medium. Rather than using the coculture grown in YPG medium, the survival rate of each strain using the coculture grown in YPD medium was estimated in the same way. Consequently, the ρ + cells of the ume6∆::KanMX strain were eliminated from the coculture over time in the absence of cycloheximide but not in the presence of 0.5 µg/mL cycloheximide when cocultivated with the ash1∆::bleMX6 mutant in YPD medium (p < 0.05) ( Figure 2C). Furthermore, similar results were observed for the ρ + cells of the gat3∆::KanMX and ume6∆::bleMX6 strains cocultivated in YPD medium with or without 30 µg/mL fluconazole (or 0.5 µg/mL cycloheximide) (data not shown). These results indicate that, in contrast to previous reports, UME6 acts as a silencer rather than an enhancer of drug resistance in ρ + cells of S. cerevisiae independent of the carbon source used in the growth medium. results were observed for the ρ + cells of the gat3∆꞉꞉KanMX and ume6∆꞉꞉bleMX6 strains cocultivated in YPD medium with or without 30 μg/mL fluconazole (or 0.5 μg/mL cycloheximide) (data not shown). These results indicate that, in contrast to previous reports, UME6 acts as a silencer rather than an enhancer of drug resistance in ρ + cells of S. cerevisiae independent of the carbon source used in the growth medium.

UME6 Is Required for the Suppression of Basal ABC Transporter mRNA Levels
Decreased drug resistance was found in the ρ + cells of the rpd3∆ strain in the spot dilution assay, while increased drug resistance in the ρ + cells of the ume6∆ strain was found in the spot dilution and cocultivation assays. These results suggested that the transcrip-

UME6 Is Required for the Suppression of Basal ABC Transporter mRNA Levels
Decreased drug resistance was found in the ρ + cells of the rpd3∆ strain in the spot dilution assay, while increased drug resistance in the ρ + cells of the ume6∆ strain was found in the spot dilution and cocultivation assays. These results suggested that the transcriptional levels of ABC transporters such as PDR5 are reduced in the ρ + cells of the rpd3∆ mutant and increased in the ρ + cells of the ume6∆ mutant. Thus, we investigated the mRNA levels of ABC transporters PDR5, PDR10, SNQ2, and YOR1 and their transcriptional regulators PDR1 and PDR3 in the ρ + cells of the wild-type, ume6∆, rpd3∆, pdr3∆, ash1∆, and gat3∆ strains grown in YPG medium to the logarithmic phase by real-time RT-PCR. PDR1, PDR3, PDR5, SNQ2, and YOR1 but not PDR10 expression was significantly induced in the ume6∆ strain compared with the wild-type strain (p < 0.05), while RPS9B expression, used as a control, was downregulated in the ume6∆ strain compared with the wild-type strain ( Figure 3A). The transcriptional induction of these ABC transporters and their transcriptional regulators can explain the increased tolerance of the ume6∆ mutant to fluconazole and cycloheximide in the spot dilution and cocultivation assays using YPG medium. In contrast, PDR1 expression, but not PDR3, PDR5, PDR10, SNQ2, or YOR1 expression, was significantly more induced in the rpd3∆ strain than in the wild-type strain (p < 0.05) ( Figure 3A). The lack of significant changes in expression of the ABC transporters, including PDR5, between the wild-type and rpd3∆ strains cannot explain the high susceptibility of the rpd3∆ mutant to fluconazole and cycloheximide in the spot dilution assays using YPG plates (Figures 1 and 3A). Therefore, the susceptibility of the rpd3∆ mutant to fluconazole and cycloheximide may result from impaired Hsp90-dependent antifungal drug resistance and ER-to-Golgi trafficking of Pdr5 in YPG medium and not from PDR5 transcriptional reduction [34,45]. In contrast to the ume6∆ mutant, the PDR5 and YOR1 mRNA levels were significantly reduced in the ash1∆ mutant compared with the wild-type strain (p < 0.05) ( Figure 3A).
Next, we investigated the mRNA levels of the ABC transporters PDR5, PDR10, SNQ2, and YOR1 and their transcriptional regulators PDR1 and PDR3 in ρ + cells of the wild-type, ume6∆, rpd3∆, pdr3∆, and ash1∆ strains grown to the logarithmic phase in YPD medium in the same way. In contrast to the results obtained by real-time RT-PCR using YPG medium, only the basal PDR5 and PDR10 mRNA levels were significantly upregulated in the ume6∆ strain compared with the wild-type strain in YPD medium (p < 0.05) ( Figure 3B). In addition, the degrees of upregulation of basal transcription of ABC transporters and their regulators in the ume6∆ strain are relatively lower in YPD medium than in YPG medium ( Figure 3A,B). However, the increased drug resistance of the ume6∆ mutant shown in the cocultivation assays using YPD can be explained by the induction in the PDR5 mRNA level. In contrast, there was no significant difference in mRNA levels of the ABC transporters and their regulators between ρ + cells of the wild-type and rpd3∆ strains grown in YPD medium (p > 0.05) ( Figure 3B). Therefore, the susceptibility of the rpd3∆ mutant to fluconazole and cycloheximide found in the spot dilution assays using YPD plates may result from impaired Hsp90-dependent antifungal drug resistance but not from the reduced PDR5 mRNA level [34]. In addition, different from the results obtained by real-time RT-PCR using YPG medium, no significant changes in expression of the ABC transporters and their regulators were found between the wild-type and the ash1∆ strains in YPD medium ( Figure 3B).  Next, we investigated the mRNA levels of the ABC transporters PDR5, PDR10, SNQ2, and YOR1 and their transcriptional regulators PDR1 and PDR3 in ρ + cells of the wild-type, ume6∆, rpd3∆, pdr3∆, and ash1∆ strains grown to the logarithmic phase in YPD medium in the same way. In contrast to the results obtained by real-time RT-PCR using YPG medium, only the basal PDR5 and PDR10 mRNA levels were significantly upregulated in the ume6∆ strain compared with the wild-type strain in YPD medium (p < 0.05) ( Figure 3B). In addition, the degrees of upregulation of basal transcription of ABC transporters and their regulators in the ume6∆ strain are relatively lower in YPD medium than Furthermore, we investigated whether transcriptional activation of the ABC transporters following cycloheximide exposure occurs in the ρ + cells of the ume6∆ mutant in YPG and YPD media using real-time RT-PCR. In YPG and YPD media, the ABC transporter mRNA levels in the ume6∆ strain at 0 min were higher than those in the wild-type strain at 0 min (p < 0.05), which is consistent with the results in Figure 3A,B ( Figure 4A,B). This also suggested that UME6 is required for the repression of basal transcription of the ABC transporters, including PDR5, in ρ + cells, independent of the media used. In addition, the degree of upregulation of PDR5 transcription in the ume6∆ strain at 0 min was relatively lower in YPD medium than in YPG medium, which is consistent with the results in Figure 3A,B ( Figure 4A,B). On the other hand, the ABC transporter mRNA lev-els, except for PDR10, in the wild-type strain were significantly higher at 45 min than at 0 min in YPG and YPD media (p < 0.05) ( Figure 4A,B). In contrast, the PDR5, PDR10, and YOR1 mRNA levels in the ume6∆ strain were not different between 0 min and 45 min in YPG medium (p > 0.05) ( Figure 4A). In YPD medium, the PDR5, but not SNQ2, mRNA levels in the ume6∆ strain were significantly higher at 45 min than at 0 min (p < 0.05) ( Figure 4B). These results suggested that UME6 is required for the intact induction of PDR5 and YOR1 transcription in ρ + cells after drug exposure in YPG medium but only for the intact induction of SNQ2 in YPD medium.
Furthermore, we investigated whether transcriptional activation of the ABC transporters following cycloheximide exposure occurs in the ρ + cells of the ume6∆ mutant in YPG and YPD media using real-time RT-PCR. In YPG and YPD media, the ABC transporter mRNA levels in the ume6∆ strain at 0 min were higher than those in the wild-type strain at 0 min (p < 0.05), which is consistent with the results in Figure 3A,B (Figure 4A,B). This also suggested that UME6 is required for the repression of basal transcription of the ABC transporters, including PDR5, in ρ + cells, independent of the media used. In addition, the degree of upregulation of PDR5 transcription in the ume6∆ strain at 0 min was relatively lower in YPD medium than in YPG medium, which is consistent with the results in Figure 3A,B ( Figure 4A,B). On the other hand, the ABC transporter mRNA levels, except for PDR10, in the wild-type strain were significantly higher at 45 min than at 0 min in YPG and YPD media (p < 0.05) ( Figure 4A,B). In contrast, the PDR5, PDR10, and YOR1 mRNA levels in the ume6∆ strain were not different between 0 min and 45 min in YPG medium (p > 0.05) ( Figure 4A). In YPD medium, the PDR5, but not SNQ2, mRNA levels in the ume6∆ strain were significantly higher at 45 min than at 0 min (p < 0.05) ( Figure 4B). These results suggested that UME6 is required for the intact induction of PDR5 and YOR1 transcription in ρ + cells after drug exposure in YPG medium but only for the intact induction of SNQ2 in YPD medium.

Discussion
In this report, we have shown that UME6 suppresses the basal transcription levels of the ABC transporters, including PDR5, and drug resistance in the ρ + cells of S. cerevisiae independent of the carbon source used in the growth medium while RPD3 does not interfere with basal PDR5 transcription but contributes to drug resistance. The results found for RPD3 in this study were consistent with previous reports [34]. However, it has not

Discussion
In this report, we have shown that UME6 suppresses the basal transcription levels of the ABC transporters, including PDR5, and drug resistance in the ρ + cells of S. cerevisiae independent of the carbon source used in the growth medium while RPD3 does not interfere with basal PDR5 transcription but contributes to drug resistance. The results found for RPD3 in this study were consistent with previous reports [34]. However, it has not been previously reported that UME6 suppresses basal PDR5 mRNA levels and drug resistance in the ρ + cells of S. cerevisiae. ρ + cells of the ume6∆ strain conferred more resistance to fluconazole and cycloheximide than those of the wild-type strain in the spot dilution assay using YPG plates but not using YPD plates. In addition, although the ρ + cells of the ume6∆ mutant were eliminated from the coculture over time in both YPG and YPD media without cycloheximide, these cells accounted for the majority of the coculture over time in YPG medium containing 0.5 µg/mL cycloheximide (Figure 2A) while maintaining a constant survival rate over time in YPD medium containing 0.5 µg/mL cycloheximide ( Figure 2C). These results can be explained by the appearance of ρ 0 cells within the ρ + cells in the wildtype and ume6∆ strains in YPD medium because ρ 0 cells of the ume6∆ mutant are more sensitive to fluconazole and cycloheximide than the ρ 0 cells of the wild-type strain [32]. Inverse transcriptional regulation of PDR5 is reported between the ρ 0 cells of the ume6∆ and ash1∆ mutants, in which the PDR3 and PDR5 mRNA levels are downregulated in the ρ 0 cells of the ume6∆ mutant but upregulated in the ash1∆ mutant [32]. In addition, the fold expression levels of most of the ABC transporters in the ume6∆ mutant compared to the wild-type strain were downregulated in YPD medium compared to YPG medium, while those in the ash1∆ mutant compared to the wild-type strain were upregulated in YPD medium compared to YPG medium ( Figure 3A,B and Figure 4A,B). Thus, this inverse transcriptional correlation in the ρ + cells of the ume6∆ and ash1∆ mutants between YPD and YPG media can also be explained by the occurrence of ρ 0 cells within the ρ + cells of the ume6∆ and ash1∆ mutants in YPD medium. As histone deacetylation leads to transcriptional repression and activation, Ume6 may serve as a silencer of PDR5 expression in ρ + cells and an enhancer of PDR5 expression in ρ 0 cells [27,47]. Furthermore, the inverse transcriptional regulation of the PDR5 between the ume6∆ and ash1∆ mutants independent of ρ + and ρ 0 cells may be caused by exclusive and elevated recruitment of the Rpd3L complex to the target regions by Ume6 in the ash1∆ mutant. Considering the above, due to the relatively low suppression levels of basal PDR5 transcription and drug resistance by UME6 in YPD medium, which could be led by the occurrence of ρ 0 cells within ρ + cells, the roles of UME6 as a negative regulator of basal PDR5 transcription and drug resistance in ρ + cells may have not been reported. In such cases, a co-cultivation assay but not a spot dilution assay may be useful for detecting a slight difference in drug resistance.
It is currently unknown how Ume6 suppresses the basal transcriptional expression of PDR5 and drug resistance in ρ + cells and inversely enhances basal PDR5 expression and drug resistance in ρ 0 cells [32]. As Ume6 binds to the promoter region of the ABC transporters such as PDR5, PDR10, and YOR1 in ρ + cells, Ume6 may directly mediate the repression of the ABC transporters by recruiting the Rpd3L and Isw2 chromatin remodeling complexes ( Figure 5) [48]. Furthermore, suppression of the ABC transporter transcription and drug resistance by Ume6 in ρ + cells may be indirectly caused by changes in the expression of the regulators of ABC transporters, such as Pdr1 and Pdr3. porters such as PDR5, PDR10, and YOR1 in ρ + cells, Ume6 may directly mediate the pression of the ABC transporters by recruiting the Rpd3L and Isw2 chromatin remodel complexes ( Figure 5) [48]. Furthermore, suppression of the ABC transporter transcript and drug resistance by Ume6 in ρ + cells may be indirectly caused by changes in the pression of the regulators of ABC transporters, such as Pdr1 and Pdr3. The transcriptional mediator complex serves as a link between sequence-spec transcription factors and the RNA polymerase II machinery [49]. There are two differ forms in the transcriptional mediator complex in S. cerevisiae: the core Mediator and Mediator. The L-Mediator complex contains the core Mediator complex and the Cd subcomplex. The Cdk8 subcomplex consists of Med12 (Srb8), Med13 (Srb9), the cyc dependent kinase Cdk8 (Srb10), and cyclin C (Srb11). The Cdk8 subcomplex has bot negative and a positive role in gene transcription [49][50][51]. Pdr1 and Pdr3 can bind to KIX domain of a mediator subunit called Med15/Gal11 of the core Mediator and L-Me ator [17]. Deletion of Med12 from the Cdk8 complex completely suppresses the induct of PDR5 expression in ρ 0 cells but not in ρ + cells, indicating a difference in the regulat The transcriptional mediator complex serves as a link between sequence-specific transcription factors and the RNA polymerase II machinery [49]. There are two different forms in the transcriptional mediator complex in S. cerevisiae: the core Mediator and L-Mediator. The L-Mediator complex contains the core Mediator complex and the Cdk8 subcomplex. The Cdk8 subcomplex consists of Med12 (Srb8), Med13 (Srb9), the cyclindependent kinase Cdk8 (Srb10), and cyclin C (Srb11). The Cdk8 subcomplex has both a negative and a positive role in gene transcription [49][50][51]. Pdr1 and Pdr3 can bind to the KIX domain of a mediator subunit called Med15/Gal11 of the core Mediator and L-Mediator [17]. Deletion of Med12 from the Cdk8 complex completely suppresses the induction of PDR5 expression in ρ 0 cells but not in ρ + cells, indicating a difference in the regulatory machinery of PDR5 transcription between ρ + and ρ 0 cells [52]. This difference may be relevant to the difference in transcriptional regulation of PDR5 by Ume6 between ρ 0 and ρ + cells.
This study indicated that UME6 is required for the intact enhancement of PDR5 and YOR1 transcription after cycloheximide exposure in YPG medium and for intact cycloheximide-induced transcription of SNQ2, but not PDR5, in YPD medium. This result suggested that basal transcriptional repression of the ABC transporters, including PDR5, by UME6 is lifted in response to drug exposure in YPG medium. However, it is unknown why the intact induction of PDR5, but not SNQ2, in the ume6∆ mutant occurs after cycloheximide exposure in YPD medium ( Figure 4B). This may result from that cycloheximide can distort measurements of mRNA levels [53]. However, which of upregulation in basal PDR5 transcription or intact drug-induced PDR5 upregulation in the ume6∆ mutant is more effective for drug resistance in YPD medium? Despite intact cycloheximide-induced transcription of PDR5 in the ume6∆ strain in YPD medium, there was no difference in cycloheximide resistance between the ρ + cells of the wild-type and ume6∆ strains in the spot dilution assay using YPD plates, while a little difference in the cocultivation assay using YPD plates. In addition, upregulation of basal PDR5 transcription levels in the ume6∆ strain compared to the wild-type strain was relatively lower in YPD medium than in YPG medium. Therefore, the upregulation of basal PDR5 transcription in the ume6∆ strain may be more important for drug resistance than intact transcriptional upregulation of PDR5 in the ume6∆ strain following cycloheximide exposure.

Conclusions
This study investigated the roles of RPD3 and UME6 in basal and drug-induced transcription of the ABC transporters, including PDR5, and PDR in the ρ + cells of S. cerevisiae. Using spot dilution and cocultivation assays and YPG and YPD media, we have shown that RPD3 contributes to drug resistance in ρ + cells, while in contrast to the previous reports, UME6 contributes to the suppression of drug resistance in ρ + cells, independent of the carbon source used in the growth medium. In addition, using a real-time PCR assay, we have shown that RPD3 does not interfere with the basal PDR5 mRNA level in ρ + cells, while UME6 is involved in the suppression of basal transcription of some ABC transporters, including PDR5, in ρ + cells regardless of the carbon source utilized. We have also described that UME6 contributes to an intact transcriptional enhancement of PDR5 in cycloheximide-exposed ρ + cells in YPG medium but not in YPD medium. This and our previous work will provide useful knowledge on yeast multidrug resistance via the transcriptional regulation of efflux genes by UME6 in ρ + and ρ 0 cells.
Funding: This work was supported by Kanazawa University.

Institutional Review Board Statement: Not applicable.
Informed Consent Statement: Not applicable.
Data Availability Statement: All data generated or analysed during this study are included in this published article.