Bacteriophages as a Strategy to Protect Potato Tubers against Dickeya dianthicola and Pectobacterium carotovorum Soft Rot

The protective effect of bacteriophage suspensions (Ds3CZ + Ds20CZ and PcCB7V + PcCB251) on phytopathogenic bacteria causing soft rot of potato tubers, namely Dickeya dianthicola (D50, D200) and Pectobacterium carotovorum (P87, P224), was observed in ex vivo and in vitro experiments. Ex vivo tests were performed (with air access) on potato slices, on cylindrical cuts from the center of the tubers, and directly in whole potato tubers. In vitro experiments were carried out in a liquid medium using RTS-8 bioreactors, where bacterial growth was monitored as optical density. In particular, the inhibitory effects of phages were confirmed in experiments on potato slices, where suppression of rot development was evident at first glance. Phage treatment against selected bacteria positively affected potato hardness. Hardness of samples treated with bacteria only was statistically significantly reduced (p < 0.05 for D50 and p < 0.001 for D200 and P87). Ex vivo experiments confirmed significant inhibition of P87 symptom development, partial inhibition of D200 and D50 in phage-treated tubers, and no effect was observed for P224. The inhibitory effect of phages against bacteria was not observed in the in vitro experiment.


Introduction
Bacterial infection of potato tubers (Solanum tuberosum L.) can occur mainly through the disruption of the tissue. The risk of mechanical damage accompanies the entire potato processing technology, from cultivation to storage or sale. The infection of potato tubers by soft rot bacteria starts in the lenticels, at the end of the stolon, or in the surface wounds of the tubers under moist conditions. Tubers can already be infected with the bacteria during harvesting, when the surface of individual tubers is disturbed, during sorting, further handling, and storage. Crop rotation in fields is important to prevent the spread of harmful agents [1][2][3][4][5].
Potato bacterial rot is most commonly caused by bacteria from the Enterobacteriaceae family of the genera Pectobacterium and Dickeya, which were previously taxonomically classified in the genus Erwinia. They are also often referred to as bacteria of the Soft Rot Pectobacteriaceae (SRP) [6,7]. These bacterial rots cause extensive damage to crops in agriculture and, in the potato sector, a total of EUR 46 million of annual loss in the European Union [8]. In addition to potatoes, they also infect other agriculturally important crops (tomatoes, corn, cabbage, carrots, peppers, beets, etc.) [9,10]. SRP cause both soft rot and a disease called "blackleg" that means blackening of the stem [11]. These bacteria produce specific pectolytic enzymes that break down the cell walls, thus, infecting the plant tissue and obtaining nutrients [12,13].

Potatoes (Samples)
Potatoes (Solanum tuberosum) grown in the Czech Republic (variety Jindra) were used to verify the inhibitory effect of the bacteriophages used. The potato tubers were grown and harvested according to European regulations. After harvesting, the potatoes were not treated in any way prior to laboratory testing. No disease symptoms were observed on the plants in the field. Before each experiment, potato tubers were surface treated (disinfected) with 3% sodium hypochlorite.

Storage and Preparation of Microbial Suspensions
For use in the experiments, the phage preparations were ten-fold diluted by adding 1 mL of phage suspension (10 8 PFU/mL) to 9 mL of saline. The saline solution was prepared by dissolving 8.5 g of NaCl (Penta; Prague, Czech Republic) and 1 g of peptone (Merck KGaA; Darmstadt, Germany) in 1000 mL of distilled water and followed by autoclaving.

Cultivation in Dents in Potato Slices
The potatoes were washed, dried, and cut into approximately 1 cm thick slices. Using a circular stainless-steel knife (diameter 1 cm), three dents (0.5 cm deep) were made in each slice near the peel. Then each potato slice was placed separately in a Petri dish. A 100 µL inoculum of the given bacterium was inoculated into dents: D. dianthicola (D50), D. dianthicola (D200), P. carotovorum (P87), or P. carotovorum (P224). Similarly, the volume of the phage inoculum was 100 µL. There were six Petri dishes per bacterial strain. Three dents were inoculated with bacteria only and three with bacteria followed by 10 7 PFU/mL of bacteriophage suspension. Slices inoculated in this way were sealed using parafilm in the Petri dishes and incubated at 25 • C inspected after 24, 48, 72, 96, and 168 h. The area infected by soft rot was quantified by image analysis (pixelation).

Cultivation by Soaking Cylinder Cuts
The second method to test the inhibitory effects of phages on bacterial growth was cultivation on cylindrical potato cuts sealed in bags. The potatoes were washed and dried. Cylindrical cuts were made in potato tubers using a 1.5 cm diameter stainless-steel circular knife. These were then cut into 3 cm lengths, washed with distilled water, dried on filter paper, and packed in polymer bags. The samples (cuts) were divided into bags of 6 pieces. The experiment was performed in six parallel repetitions, with a total of 12 bags per bacterium. The first half was inoculated with bacteria only and the second half with bacteria and phages. The prepared bags were placed for cultivation in a thermostat at 25 • C.
Selected potato quality parameters were evaluated after 24, 48 and 72 h. Control samples were evaluated at the same time intervals. The effect of phages on the selected bacteria was evaluated by the change in texture (hardness) of potato tubers, the color change, and the effect of phage suspensions on the growth curves (in vitro) of individual bacteria.

Cultivation in Liquid Broth
Bacterial growth in the presence or absence of phages was monitored and evaluated using the Biosan RTS-8 bioreactor (Biosan; Riga, Latvia). Approximately 15 mL of PDB was transferred to 50 mL centrifuge tubes, into which 100 µL of bacterial suspension (1.2 × 10 9 CFU/mL) with/without 1000 µL of bacteriophages (10 7 PFU/mL) (multiplicity of infection, MOI = 0.1) was added. Thus, the measurements were carried out in two parallel measurements, with one tube containing the selected bacterial strain and the other tube containing the appropriate bacteriophages added. The tubes thus prepared were placed in a bioreactor and cultured at 25 • C with a speed of 1000 rpm for approximately one week, depending on the particular growth process.

Whole Tuber Cultivation
The inhibitory effect of 100 µL (10 7 PFU/mL) bacteriophages (MOI = 0.01) on bacterial growth inside potato tubers was evaluated by excavating a hole in the core of the tuber with a 0.6 cm diameter corkscrew and then inoculating the tubers with bacteria or bacteria and phages. This part of the experiment was conducted on only one set of samples, in order to give the reader an idea of the development of soft rot in the potato tuber. Incubation was carried out for one week at 25 • C. After 7 days, the potatoes were cut into half and the extent of bacterial rot was visually assessed. The area infected by soft rot was quantified by image analysis (pixelation).

Texture Measurement
The change in texture during the bacterial rot development was measured instrumentally using an Instron Model 5544 (Instron Ltd.; High Wycombe, Great Britain) instrument via the penetration method in cylindrical samples (cut 1.5 cm diameter and 1.5 cm in length). A 2 mm diameter probe with a penetration rate of 80 mm/min was used to penetrate the samples. A minimum of eight maximum penetration force (N) values was measured for each sample. The measured data were then evaluated statistically and their arithmetic means were plotted on graphs.

Color Measurement
During the development of bacterial rot, color change was monitored in samples by measuring reflectance in the 360-740 nm color spectrum on a Minolta CM-5 spectrophotometer (Konica Minolta; Tokyo, Japan) and processed by SpektraMagic NX Pro (Konica Minolta; Tokyo, Japan). The color values were given by the CIE-L*a*b* color space, which is typically an imaginary color space created mathematically. The values indicate: L*-the color coordinate between black (0%) and white (100%). a*-coordinates of the color between red (−values) and green (+values). b*-coordinates of the color between yellow (−values) and blue (+values). Measurements were taken at the standard viewing angle (10 • ), with a daylight source D65, through a 3 mm slit and at laboratory temperature. The instrument was calibrated before each measurement (white and black colors). Due to non-significant differences within parallel samples on the same day, measurements were always made in four bags, two inoculated with bacteria only and two inoculated with phage as well. Ten values were measured for each bag. Because the samples were in the package, the corrected SCI (specular component included) reflectance was observed for the L*, a*, b*, and color spectrum values. The final measurement value for each sample was calculated as the average of all measured data, where outliers according to the Dean-Dixon test were not included. The general comparison of the visible color change at the beginning of the Microorganisms 2022, 10, 2369 5 of 14 experiment and during the development of bacterial rot was evaluated using the value of the color change coefficient ∆E (see Equation (1)) according to [32]: where ∆L*, ∆a*, and ∆b* are differences in color parameters between control and PEFtreated samples.

Statistics
The Statistica 12.0 software (StatSoft; Prague, Czech Republic) was used for statistical data analysis. To test the assumption of normality, the Shapiro-Wilk and Dean-Dixon tests were applied to the collected data. Obtained data are presented as mean ± SD (standard deviation). A one-way post hoc analysis of variance (ANOVA) was performed on the collected data to determine if each of the selected variables (penetration force, coordinates L*, a*, and b*) changed during storage. The variable ∆E was not included in the ANOVA test because ∆E is strongly dependent on L*, a*, and b*. The HSD Tukey test was then used to compare the data between each day of sample storage. The significance level was set at p = 0.05. Similarly, statistics were also performed between phage-treated and non-phage-treated samples to determine if phage treatment affects the above parameters.

Results and Discussion
The first experiment in this research was to evaluate the effect of bacteriophages on selected potato tuber rot bacteria on potato slices. For this assessment, the method of creating dents in potato slices was used, into which bacteria or bacteria and subsequently (after 10 min) bacteriophages were inoculated. Figure 1 shows the progression of bacterial rot caused by P. carotovorum P87 during (index 1) after 96 h compared to potatoes treated with bacteria and bacteriophages (index 2). As can be seen, the phage suspension inhibited the growth of P87 throughout the entire (72 h) storage experiment. In the first 48 h of storage, it is already possible to see the development of soft rot on non-treated potato slices, which accounts for 37.2 ± 1.1% of the surface area ( Figure 2). At the end of the experiment, the maceration zone already accounted for 56.6 ± 12.4% of the area, compared to the phage-treated samples. For the slices treated with phage suspensions, the rot formed approximately 9.4 ± 2.2% of the total area during 96 h. While the inhibitory effect of phages on the activity of bacteria can be observed on slices treated with bacteriophage as well, the slices are shriveled but do not show signs of bacterial rot. The visible discoloration of the mesh on the tuber slice in the vicinity of the puncture could be due to oxidation and suberization. Figure 3 shows the growth of different bacterial strain rot after 72 h at 25 • C on slices inoculated with bacterial suspension and bacterial suspension treated with bacteriophages. The visible discoloration of the mesh on the tuber slice in the vicinity of the puncture could be due to oxidation and suberization. However, it is evident from the figure that there was a slowing down and drying of the tuber rot (reduction in the extent of mesh maceration). Statistical evaluation shows a significant improvement in observed parameters between treated/untreated tubers. The most significant increase (72 h at 25 • C) in bacterial rot was observed for D. dianthicola D50 (p < 0.01), which infected 64.1% of the potato tuber area. In all observed bacterial strains, the macroscopic manifestations of rotting can be observed, with a gradual decomposition of the potato tissue and its discoloration, which is markedly dark at the edges. This discoloration was probably caused due to air access. The positive effect (p < 0.05) on preventing rot growth on potato slices was also confirmed by pixelation of soft rot, expressed as maceration zones (%) in Figure 4. Experimental data showed that the mixture of isolated lytic phages at a concentration of 10 7 PFU/mL effectively suppressed potato soft rot caused by D. dianthicola and P. carotovorum. Phagetreated samples showed almost no signs of rotting processes. The phage solutions reduced the development of disease symptoms (measured as % of macerated area) by more than Microorganisms 2022, 10, 2369 6 of 14 90% or stopped the development completely. These results are consistent with a study by Muturi et al. (2019), which tested the effect of bacteriophages isolated from soil and water samples against the growth of P. carotovorum [33]. In their case, soft rot symptoms were reduced by more than 90% when a mixture of bacteriophages at a concentration of 10 9 PFU/mL was applied. Similarly, Czajkowski et al. (2014) confirmed the inhibitory and lytic effects of bacteriophages on the control bacterium D. solani [34].   Figure 3 shows the growth of different bacterial strain rot after 72 h at 25 °C on slices inoculated with bacterial suspension and bacterial suspension treated with bacteriophages. The visible discoloration of the mesh on the tuber slice in the vicinity of the puncture could be due to oxidation and suberization. However, it is evident from the figure that there was a slowing down and drying of the tuber rot (reduction in the extent of mesh maceration). Statistical evaluation shows a significant improvement in observed parame-   Figure 3 shows the growth of different bacterial strain rot after 72 h at 25 °C on slices inoculated with bacterial suspension and bacterial suspension treated with bacteriophages. The visible discoloration of the mesh on the tuber slice in the vicinity of the puncture could be due to oxidation and suberization. However, it is evident from the figure that there was a slowing down and drying of the tuber rot (reduction in the extent of mesh maceration). Statistical evaluation shows a significant improvement in observed parame- reduced the development of disease symptoms (measured as % of macerated area) by more than 90% or stopped the development completely. These results are consistent with a study by Muturi et al. (2019), which tested the effect of bacteriophages isolated from soil and water samples against the growth of P. carotovorum [33]. In their case, soft rot symptoms were reduced by more than 90% when a mixture of bacteriophages at a concentration of 10 9 PFU/mL was applied. Similarly, Czajkowski et al. (2014) confirmed the inhibitory and lytic effects of bacteriophages on the control bacterium D. solani [34].   more than 90% or stopped the development completely. These results are consistent with a study by Muturi et al. (2019), which tested the effect of bacteriophages isolated from soil and water samples against the growth of P. carotovorum [33]. In their case, soft rot symptoms were reduced by more than 90% when a mixture of bacteriophages at a concentration of 10 9 PFU/mL was applied. Similarly, Czajkowski et al. (2014) confirmed the inhibitory and lytic effects of bacteriophages on the control bacterium D. solani [34].   The most important characteristic observed in the experiment, where potatoes were inoculated by soaking, was the textural properties ( Figure 5) of the samples (hardness). In addition, their color change was also assessed (Table 1). However, these characteristics are not necessarily indicative of the development of bacterial rot. Figure 5 shows that during the rotting process caused by D. dianthicola D50, the textural properties of the potato deteriorate and, therefore, the force required to penetrate the sample decreases. From these observations, it can be concluded that treatment of potato tissue with bacteriophages mitigates the symptoms of rotting by slowing down the decomposition reactions caused by bacterial enzyme-producing pathogens. The measured penetration force data for samples inoculated only with D50 bacteria were statistically different (p < 0.001) after 24 h compared to the values measured at the beginning of the experiment, when the penetration force values were F = 7.40 ± 0.45 N for the control samples and F = 5.30 ± 0.58 N for the samples inoculated with D50 bacteria. For the bacteriophage-treated samples, the penetration force value was F = 7.04 ± 0.87 N and was not statistically different compared to the control data (p > 0.05) but was statistically different compared to the parallel samples not inoculated with phage (p < 0.05). The penetration force measured after 72 h was statistically different (p < 0.001) from the values at the beginning of the experiment for both phage-untreated and phage-treated samples; the penetration force for samples inoculated with bacteria was 0.11 ± 0.05 N and for the samples inoculated with both bacteria and bacteriophage was 2.57 ± 0.69 N. The measured values of the experiments were also statistically different (p < 0.01) from each other. This fact again confirms that the bacteriophages in this experiment do not explicitly prevent the decomposition of potato tissue but slow down or mitigate the potato decomposition. Similar results were observed for the bacterium D200. The penetration force (7.44 ± 0.45 N in the beginning) gradually decreased more significantly in bacteria-treated potatoes compared to phage-treated potatoes (p < 0.001). The hardness of D200-inoculated potatoes after 72 h was then 0.22 ± 0.09 N and phage-treated potatoes 5.00 ± 0.49 N. The bacteria P87 and P224 also showed a statistically significant decrease after 72 h (p < 0.01) in penetrating power during the experiment. However, the hardness of the potatoes did not decrease as much as in potatoes inoculated with D50 and D200 bacteria. Similar results to D50 and D200 were observed for P87, where phage treatment positively affected potato texture. However, positive results were not found for bacterium P224. The hardness of the phage-treated potatoes at the end of the experiment was 2.05 ± 0.85 N, which is even lower compared to the control samples, where a penetration force of 4.29 ± 0.65 N was measured. Thus, the results of the penetration force indicate that phages have a positive effect on potato hardness during storage. In all cases, the structure of a potato inoculated with bacteria was destroyed. Unfortunately, it is not possible to compare these results with scientific studies because, to our knowledge, no such studies are available. The most important characteristic observed in the experiment, where potatoes were inoculated by soaking, was the textural properties ( Figure 5) of the samples (hardness). In addition, their color change was also assessed (Table 1). However, these characteristics are not necessarily indicative of the development of bacterial rot.    Values followed by a small index in the rows (within the parameters L*, a*, and b*) differ significantly (p < 0.05) during storage and values followed by a large index in the columns (within the difference between treated and untreated potatoes) differ significantly (p < 0.05) (Tukey HSD test in one-way ANOVA, separately for each bacterial species). Figure 6 shows the growth of bacterial rot in whole potato tubers, phage treated or non-treated, after 72 h at 25 • C. There is less soft rot (mesh maceration) on phage-treated potato tubers. On these tubers, there is visible discoloration and drying of the rot, which makes the rot darker and more pronounced. The most pronounced inhibitory effect was observed with bacterium P87 (p < 0.05), followed by bacteria D200 and D50 (p < 0.05). The phage suspension reduced the symptoms of P87 rot by 83.9% and 42.3-77.6% for D50 and D200 bacteria, respectively. The last sample is a potato treated with P. carotovorum P224, where no significant differences are visible between phage-treated and untreated tubers (p > 0.05). Areas of mesh maceration by individual bacterial rots can be seen in Figure 7. Another option could be to inoculate potatoes by dipping them in appropriate suspensions of bacteria and bacteriophages. Soaking of whole tubers was carried out in a study by Hassan et al. (2017), where out of nine bacteriophage isolates, four showed inhibitory effects against all tested P. carotovorum strains [35]. In a study by Bugaeva et al. (2021), they applied phages in the form of regular spraying to potatoes stored in storage and maintained also by conventional measures, such as maintaining temperature and relative humidity [36]. According to their results, by applying bacteriophages, they were able to reduce the concentration of bacteria in tubers, but due to the effectiveness of storage conditions, the study is not completely conclusive in terms of suppression of disease symptoms by bacteriophages. In a study by Zaczek-Moczydłowska et al. (2020), they tested the efficacy of selected phages to inhibit Pectobacterium spp. in ex vivo experiments by incubating tubers surface inoculated with bacteria or phages cut in half [37]. For evaluation, they compared the initial tuber weight and the tuber weight at the end of the experiment after removal of the macerated mass.
0, x FOR PEER REVIEW 11 of 15 treated samples either. From the measured data of color parameters, the color change coefficient ∆E was calculated, always between the values at the beginning of the measurement and 24 h of storage, respectively 48 and 72 h. From the measured data of color parameters, the color change coefficient ∆E was calculated, always between the values at the beginning of the measurement and 24 h of storage, respectively, 48 and 72 h. As can be seen in Table 1, the color change coefficients also confirm the color change in the potato after treatment during storage. Within 24 h of storage, a color change occurred, with the ∆E coefficient for the measured samples ranging between 3.8 and 14.4. Shevell (2003) defined the range of ∆E coefficient with a range from 2 to 10 as a color change that can be observed at the first glance of the observer [32] and the range of ∆E coefficient range 11-49 as colors similar to their opposite. The ∆E coefficients were also calculated to compare the color of potatoes treated with bacteria alone and those treated with bacteria and phages. In this case, the range of ∆E was from 1.1 to 6.9. The highest value of the ∆E coefficient was calculated for bacterium D50 after 24 h of storage. Again, these values lie in a range of 2 to 10 according to Shevell (2003). Thus, it can be said that there is a color difference between bacteria-treated and phage-treated potatoes.  Based on the determination of the color and the color coefficients L*, a*, and b*, respectively (Table 1), it can be seen that the color of the potatoes changed during the storage experiment. In particular, the most significant changes were observed for the lightness value L*, where there was a significant increase in lightness (p < 0.01) in all cases for all tested potatoes treated with both bacteria only and bacteria with phages compared to the control samples at the beginning of the experiment. A similar trend of color changes within the values of parameters a* and b* was also observed. Significant changes were observed, especially for the parameter a* in potatoes treated with D50 bacteria (p < 0.05). On the contrary, no significant differences (p > 0.05) were observed for the b* parameter in potatoes treated with bacteria P87, respectively, P87 + P. Significant statistical differences in color coordinators, except for L* and a*, were also not detected for P224 during storage. No significant statistical differences were shown between control and P224-treated samples either. From the measured data of color parameters, the color change coefficient ∆E was calculated, always between the values at the beginning of the measurement and 24 h of storage, respectively 48 and 72 h. From the measured data of color parameters, the color change coefficient ∆E was calculated, always between the values at the beginning of the measurement and 24 h of storage, respectively, 48 and 72 h. As can be seen in Table 1, the color change coefficients also confirm the color change in the potato after treatment during storage. Within 24 h of storage, a color change occurred, with the ∆E coefficient for the measured samples ranging between 3.8 and 14.4. Shevell (2003) defined the range of ∆E coefficient with a range from 2 to 10 as a color change that can be observed at the first glance of the observer [32] and the range of ∆E coefficient range 11-49 as colors similar to their opposite. The ∆E coefficients were also calculated to compare the color of potatoes treated with bacteria alone and those treated with bacteria and phages. In this case, the range of ∆E was from 1.1 to 6.9. The highest value of the ∆E coefficient was calculated for bacterium D50 after 24 h of storage. Again, these values lie in a range of 2 to 10 according to Shevell (2003). Thus, it can be said that there is a color difference between bacteria-treated and phage-treated potatoes.
range of 2 to 10 according to Shevell (2003). Thus, it can be said that there is a color difference between bacteria-treated and phage-treated potatoes.   The results of the in vitro experiment can be seen in Figure 8. The efficacy of selected lytic phages (10 7 PFU/mL) on bacteria was assessed in a dynamic environment where the medium was mixed at a rotation of 1000 rpm. The in vitro experiment in liquid medium (tryptic soy broth, TSB) was performed in a laboratory bioreactor RTS-8, evaluating the optical density of the solution (broth).
The results of in vitro measurements in liquid medium suggest opposite effects of phages than experiments directly on potato samples. The addition of bacteriophages to the suspension of all tested bacteria shortens the lag phase of growth and increases the overall bacterial growth, as can be seen in Figure 8. The resulting optical density after 50 h was approximately 3-times higher for D50 and D200 bacteria treated with phages compared to untreated samples. For bacteria P87 and P224, it was then approximately 30% higher. As we can hypothesize from our measured results, bacteriophages are unable to inhibit bacteria in a constantly rotating liquid environment. These results are in line with those obtained by Bartnik et al. (2022), where successful inhibition of D. solani was also not confirmed in an in vitro experiment [38]. In a study by Zaczek-Moczydłowska et al. (2020), a bacteriophage mixture was tested in vitro by mixing 100 µL of a bacterial suspension of Pectobacterium spp. at a concentration of 10 8 CFU/mL, with 4 mL of a phage mixture at a concentration of 10 8 PFU/mL [37]. The growth curves of the bacteria were measured using optical density at 600 nm. Measurements were taken every hour for the first five hours and then after 24 h. As a result, the OD of the bacteriophage-treated samples was reduced, thus confirming the inhibitory effect, even in liquid medium. The study does not elaborate on how the samples were incubated, but it can be assumed that they were in a static environment. This would confirm our hypothesis that bacteriophages are unable to infect bacteria under constant rotation of the system. A similar experiment was performed by Soleimani-Delfan et al. (2015) but against Dickeya spp., with the difference that the samples were incubated on a rotary shaker at 120 rpm. Spectrophotometric optical density measurements showed inhibitory effects of bacteriophages [39]. In the current experiment, the rotational speed was much more intense (1000 rpm), so a reduction could lead to more promising results.
oorganisms 2022, 10, x FOR PEER REVIEW 12 of The results of the in vitro experiment can be seen in Figure 8. The efficacy of select lytic phages (10 7 PFU/mL) on bacteria was assessed in a dynamic environment where medium was mixed at a rotation of 1000 rpm. The in vitro experiment in liquid mediu (tryptic soy broth, TSB) was performed in a laboratory bioreactor RTS-8, evaluating optical density of the solution (broth). The results of in vitro measurements in liquid medium suggest opposite effects phages than experiments directly on potato samples. The addition of bacteriophages the suspension of all tested bacteria shortens the lag phase of growth and increases overall bacterial growth, as can be seen in Figure 8. The resulting optical density after h was approximately 3-times higher for D50 and D200 bacteria treated with phages co pared to untreated samples. For bacteria P87 and P224, it was then approximately 3 higher. As we can hypothesize from our measured results, bacteriophages are unable inhibit bacteria in a constantly rotating liquid environment. These results are in line w those obtained by Bartnik

Conclusions
A positive inhibitory effect of bacteriophages against selected phytopathogens was observed in ex vivo experiments. On potato slices, there was a visible inhibition of bacterial rot growth in all cases and, on whole tubers, there was a successful inhibition of bacteria, especially for P87, while no effect was observed against P224. The phage treatment of potatoes also had a positive effect on potato hardness. The hardness of potatoes inoculated with bacteria alone decreased significantly within 72 h. Color changes were also observed during storage, but it is questionable whether this is sufficient evidence of a positive effect of phages against bacteria. Experiments in liquid medium incubated in vitro in the bioreactor indicated opposite effects of phage on bacteria compared to ex vivo experiments. From the above, it appears that effective bacteriophage applications will not include dynamic methods (e.g., washing or floating potatoes). Static application methods, such as sprinkling or spraying treatment with phage suspension, may be more effective.

Institutional Review Board Statement:
The study did not require ethical approval.
Data Availability Statement: Data available on request due to privacy restrictions. The data presented in this study are available on request from the corresponding author.