Biocontrol Potential of Chitin and Chitosan Extracted from Black Soldier Fly Pupal Exuviae against Bacterial Wilt of Tomato

Globally, Ralstonia solanacearum (Smith) is ranked one of the most destructive bacterial pathogens inducing rapid and fatal wilting symptoms on tomatoes. Yield losses on tomatoes vary from 0 to 91% and most control measures are unaffordable to resource-poor farmers. This study investigated the antimicrobial activities of chitin and chitosan extracted from black soldier fly (BSF) pupal exuviae against R. solanacearum. Morphological, biochemical, and molecular techniques were used to isolate and characterize R. solanacearum for in vitro pathogenicity test using disc diffusion technique. Our results revealed that BSF chitosan significantly inhibited the growth of R. solanacearum when compared to treatments without chitosan. However, there was no significant difference in the antibacterial activities between BSF and commercial chitosan against R. solanacearum. Soil amended with BSF-chitin and chitosan demonstrated a reduction in bacterial wilt disease incidence by 30.31% and 34.95%, respectively. Whereas, disease severity was reduced by 22.57% and 23.66%, when inoculated tomato plants were subjected to soil amended with BSF chitin and chitosan, respectively. These findings have demonstrated that BSF pupal shells are an attractive renewable raw material for the recovery of valuable products (chitin and chitosan) with promising ability as a new type of eco-friendly control measure against bacterial wilt caused by R. solanacearum. Further studies should explore integrated pest management options that integrate multiple components including insect-based chitin and chitosan to manage bacterial wilt diseases, contributing significantly to increased tomato production worldwide.


Introduction
The bacteria Ralstonia solanacearum (Smith) is a soil-borne plant pathogen that affects mainly solanaceous plants and some non-solanaceous plants [1]. They are Gram-negative, short rod, strict aerobe, non-spore-forming, motile bacteria. Molecular characterization of R. solanacearum strains based on sequence analysis of internal spacer (ITS) region has resulted in four phylotypes divisions [2]. Ralstonia solanacearum has been reported to be the causal agent of bacterial wilt disease in over 200 host plant species belonging to 50 plant families [3]. These bacteria infect plants from planting media through root injuries caused either by mechanical, insect, or nematode damage [4]. The bacteria has been reported to

Study Site
This study was conducted at Jomo Kenyatta University of Agriculture and Technology (JKUAT), Juja Kiambu County (1.0891 • S, 37.0105 • E, altitude of 1400 asl). In vitro and field tests were done at the Department of Botany laboratory and Department of Horticulture and Food Security greenhouse, respectively. The pupal exuviae from which both chitin and chitosan were extracted was obtained from the BSF mass production facility at the Animal Rearing and Containment Unit (ARCU) at the International Centre of Insect Physiology and Ecology (icipe), Nairobi, Kenya.

Soil Sample Collection and Isolation of Ralstonia solanacearum
Soil samples were randomly collected from tomato growing fields that had dead tomato plants and those depicting wilting symptoms at Jomo Kenyatta University of Agriculture and Technology (JKUAT). Blocks were earmarked for the trial then divided into plots. Three independent plots were selected then subdivided into numbered units. Three units per plot were randomly selected and soils collected (in triplicates) by digging around the plant roots at 30 cm depth using a sterile jembe. Ten grams of the sampled soil was suspended in 100 mL of sterile distilled water then agitated for 10 min on a mechanical shaker. Serial dilution was performed by transferring 1 mL of the suspension into 9 mL of sterile distilled water in test tubes to dilution factor 10 −6 . Plating was done by pipetting and spreading 0.1 mL of the suspension from each dilution tube onto sterile Triphenyl tetrazolium chloride (TZC) agar plates. The plates were then incubated at 37 • C for 24 h after which bacterial growth was measured by counting colonies of R. solanacearum and documented.

Cultural Characterization
Typical cultural characteristics of R. solanacearum were determined using 24 h old cultures grown at 37 • C on TZC gar according to the procedure done by [22]. The fluidal, irregularly round colonies with white margins and light pink centers were considered to be R. solanacearum colonies [23,24] and were sub-cultured to obtain pure cultures for subsequent use.

Morphological Characterization
Gram staining of pure cultures of the R. solanacearum was done following the procedure described by [25]. This involved spreading a loopful of the colonies on a glass slide and heatfixing on a very low flame. Aqueous crystal violet solution was spread over the smear and left for 30 s then washed with running tap water for one minute. It was then flooded with iodine and left for one minute, rinsed with tap water, then decolorized with 95% ethanol until clear runoff. After washing, the smear was counter-stained with safranin for 30 s, washed with tap water, dried, and observed at a magnification at ×100 using oil immersion. Pink short rod-shaped cells were considered a positive test for R. solanacearum [26].

Biochemical Characterization
The bacterial solubility in 3% KOH was examined to eliminate any possible confusion of the bacterial pathogens that cause wilting in tomatoes as described by Khasabulli et al. (2017). The pure culture of the pathogen was picked using a sterile wire loop and placed on the glass slide containing a drop of 3% KOH solution. It was stirred for about 10 s then raised for a few centimeters from the slide while observing for mucoid/slime threads. The formation of a viscous solution or slime thread indicated a positive test (KOH soluble). A catalase test was done as described by Khasabulli et al. (2017), whereby a loopful of fresh bacterial culture of R. solanacearum was mixed with a drop of 3% hydrogen peroxide (H 2 O 2 ) on a glass slide and observed for the production of gas bubbles. An observation of effervescence suggested a positive test [23,25]. A gas production test for the pure cultures of isolated R. solanacearum was conducted according to the procedure done by Pawaskar et al. (2014). Sterile nutrient broth with 2% glucose in test tubes containing inverted Durham tubes was used. The test tubes were inoculated with 0.5 mL of bacterial suspension and incubated at 37 • C for 24 h. The presence of air bubbles in the inverted Durham tube was an indication of gas production hence considered a positive test. The ability of R. solanacearum isolate to hydrolyze starch in nutrient agar was tested according to the procedure done by Pawaskar et al. (2014). The bacterial cultures of R. solanacearum were inoculated and spread on the center of sterile nutrient agar plates containing 0.02% starch then incubated at 37 • C for 24 h. After incubating, the plates were flooded with Lugol's iodine. A clear zone around bacterial culture suggested a positive test [25,27].

Molecular Characterization
The genomic DNA was extracted using Quick-DNA TM Fungal/Bacterial Miniprep kit (Zymo Research Corp, Irvine, CA, USA). A pure culture of R. solanacearum was sub-cultured on Casamino acids Peptone Glucose (CPGA) plates and incubated overnight then used for extraction of DNA based on the manufacturer's instructions. The extracted DNA was run under 1% agarose gel electrophoresis and visualized on an ultraviolet Transilluminator (UVT-20 SML model). Multiplex PCR was performed for phylotype identification and confirmation of the isolated R. solanacearum diseased plants from JKUAT fields using phylotype-specific primers as described by Fegan and Prior (2005). The reaction was carried out in a total volume of 25 µL reaction containing: One Taq 2X Master mix, phylotypespecific forward primers; Nmult:21:1F, Nmult:21:2F, Nmult:22: lnF, Nmult:23:AF, reverse primer Nmult:22:RR, DNA template, and Taq DNA polymerase. Amplifications were done in an Eppendorf AG, 22331 Hamburg, Germany thermocycler using the following conditions: initial denaturation at 94 • C for 3 min, 35 cycles of denaturation at 94 • C for 30 s, annealing at 55 • C for 30 s, extension at 72 • C for 30 s, and a final extension step at 72 • C for 7 min. The amplified PCR products were run through electrophoresis on 1% (w/v) agarose gel and visualized on a UV transilluminator [28].

Pathogenicity Test
A pathogenicity test was conducted on one-month-old healthy tomato seedlings (Prostar F1) using R. solanacearum from the International Centre of Potato Research (CIP) and those isolated from JKUAT fields. The soil drench method was used for inoculation of the plants with the pathogen according to the procedure by Kariuki (2020). The seedlings were transplanted onto plastic pots containing autoclaved cocopeat mixed with Hoagland's solution. The plants were left in the medium for three days to let them stabilize. After three days of stabilization in the medium, the seedlings were injured on the stems just above the growth media surface and the roots using a sterile scalpel blade. Turbidity of bacterial suspension was adjusted to 0.5 McFarland equivalents then 10 mL drenched on the injured stems and roots using a sterile syringe. A set of transplanted seedlings drenched with sterile distilled water served as the control. Each treatment consisted of 10 plants one per pot and the experiment was done in triplicates. The plants were then monitored for bacterial wilt symptoms in the greenhouse under natural sunlight conditions. Plants with at least one wilted leaf were considered diseased. After the development and appearance of wilting symptoms, the pathogen was reisolated on TZC media and re-identified using cultural, morphological, biochemical, and molecular methods [29].

Extraction of Chitin and Chitosan
Chitin and chitosan were extracted from the BSF pupal exuviae using chemical methods as described by . The pupal exuviae were sorted, washed thoroughly with tap water, and rinsed twice with distilled water, sun-dried, and ground into a fine powder using a blender. The obtained pupal exuviae powder was first demineralized by treating 100 g of the powder with 1000 mL of 1 M hydrochloric acid then boiled for two hours to remove all the minerals. The demineralized material was then washed with distilled water until a neutral pH was achieved, then filtered and dried in an oven at 60 • C for 6 h. The dried demineralized powder weighing 100 g was then treated with 1000 mL of 1 M sodium hydroxide (NaOH) then boiled for 4 h to remove proteins. The extracted chitin material was washed thoroughly with distilled water until it attained a neutral pH and dried in an oven at 60 • C for 6 h. Thereafter, the chitin was further processed to produce chitosan as described by  but with slight variations. One hundred grams of dried chitin was refluxed in 1000 mL of 40% NaOH then boiled for 8 h with continuous stirring. The obtained product was washed thoroughly with distilled water to a neutral pH then dried in an oven at 60 • C. Chitosan (CHT) extracts weighing 0.5, 1, 2.5, and 5 g were dissolved in 100 mL of 1% (v/v) acetic acid solution separately to obtain different concentrations of CHT (w/v). Following the dissolution of chitosan in acetic acid, it was sterilized by autoclaving at 121 • C for 15 min and stored at 4 • C for later use [30,31].

Antimicrobial Effects of BSF Chitosan on Ralstonia solanacearum
Antimicrobial activities of BSF chitosan against R. solanacearum were tested using disc diffusion [32]. Pure cultures of R. solanacearum were sub-cultured overnight at 37 • C on casamino peptone glucose (CPG) agar media. Bacterial colonies from the plates were suspended in sterile normal saline and adjusted to obtain an equivalent of 0.5 McFarland. then pipetted onto 20 mL of Mueller Hilton Agar plates. L-form glass spreader was sterilized by passing over a flame and used to spread the bacterial suspension over the Agar plates. Sterile Whatman's filter paper discs of 6 mm diameter were soaked in different concentrations (0, 0.5, 1, 2.5, and 5%) of sterilized BSF chitosan. Chitosan-soaked discs were air-dried then gently picked using sterile forceps and placed on the surface of inoculated agar plates at equidistant positions. Filter paper discs soaked in sterile distilled water were used as the negative control. The plates were incubated at 37 • C for 24 h The inhibition zones were measured in millimeters and average inhibition zones were calculated for the three replicates. The "control treatment" consisted of different concentrations of commercial chitosan (0, 0.5, 1, 2.5, and 5%) and eight different antibiotic discs: Ampicillin (25 µg), Tetracycline (100 µg), Nitrofurantoin (200 mcg), Nalidixic acid (30 µg), Streptomycin (25 µg), Sulphamethoxazole (200 µg), and Cotrimoxazole (25 µg) [33].

Effects of BSF Chitosan on Ralstonia solanacearum Symptom Expression in Tomato
Tomato seeds (Prostar F1 from Simlaw Seeds Company, Nairobi, Kenya) were grown in seed propagating units for 30 days. The seedlings were then transplanted onto plastic pots containing 200 g of sterile growth media (cocopeat) and 20 g of BSF-derived chitin and chitosan separately. Three days after transplanting 50 mL of Hoagland's solution was added to the transplanted plants. Fourteen days after transplanting, the plants were inoculated by drenching 10 mL of 0.5 McFarland suspension of R. solanacearum. The plants were injured using sterile scalpel around roots and on stem slightly above the growth media surface. The pathogen was drenched around the injured surface using a sterile syringe. Seedlings transplanted into sterile growth media devoid of chitin or chitosan extracts and inoculated with R. solanacearum served as the positive control. Other seedlings transplanted onto growth media without chitin or chitosan and not inoculated with R. solanacearum bacterial suspension served as the negative control. The experiments were replicated three times and arranged in a completely randomized design in the greenhouse under natural light conditions. The plants were irrigated after every 12 h and other standard agronomic practices were observed to ensure that plants were free from any form of stress.
Bacterial wilt disease incidence (DI) was monitored daily for 1 month after pathogen inoculation. Plants with wilted leaves were recorded as diseased plants. The percent disease incidence was calculated using the formula by Kempe and Sequeira (1983): where n = Number of wilted leaves per plant, N = total number of leaves. Bacterial wilt disease severity was evaluated based on the scale (0-5) of Kempe and Sequeira (1983) where 0 = no symptoms; 1 = 1-25% leaves wilted; 2 = 26-50% leaves wilted; 3 = 51-75% leaves wilted; 4 = more than 75% but less than 100% of leaves wilted; 5 = all leaves wilted and plant death.
The percent disease severity (DS) was calculated using the formula [34]: where ni = number of plants with the respective disease rating; vi = disease rating; V = the highest disease rating; and N = the number of plants observed

Data Collection and Analysis
Data on cultural, biochemical, and molecular characterization were collected by observation, and the results were recorded on excel sheets. The data on antimicrobial activity was collected by measuring clear zones of inhibition around each disc on each plate of each test organism. All experimental data were expressed as means ± standard error. Data analysis was done using Stata SE-64 2011 statistics software and means separated using the Bonferroni range test. The difference in mean inhibition zone was done using a one-way analysis of variance, and the difference was considered significant at p ≤ 0.05.

Characterization of Ralstonia solanacearum
Kelzman's tetrazolium chloride (TZC) media-enabled successful isolation of R. solanacearum from the soil sample collected from the JKUAT farm and differentiation of virulent and avirulent strains. Most colonies on the observed plates appeared as large, fluidal irregularly round white with a pale red to the pink center on TZC media hence as per Balabel (2018) they were virulent [35] (Figure 1).
conditions. The plants were irrigated after every 12 h and other standard agronom tices were observed to ensure that plants were free from any form of stress.
Bacterial wilt disease incidence (DI) was monitored daily for 1 month after pa inoculation. Plants with wilted leaves were recorded as diseased plants. The perce ease incidence was calculated using the formula by Kempe and Sequeira (1983): % DI= ×100 where = Number of wilted leaves per plant = total number of leaves.

Data Collection and Analysis
Data on cultural, biochemical, and molecular characterization were collected servation, and the results were recorded on excel sheets. The data on antimicrobial a was collected by measuring clear zones of inhibition around each disc on each p each test organism. All experimental data were expressed as means ± standard erro analysis was done using Stata SE-64 2011 statistics software and means separated the Bonferroni range test. The difference in mean inhibition zone was done using way analysis of variance, and the difference was considered significant at p ≤ 0.05.

Characterization of Ralstonia solanacearum
Kelzman's tetrazolium chloride (TZC) media-enabled successful isolation of nacearum from the soil sample collected from the JKUAT farm and differentiation o lent and avirulent strains. Most colonies on the observed plates appeared as large, irregularly round white with a pale red to the pink center on TZC media hence Balabel (2018) they were virulent [35] (Figure 1 The observation of Gram staining reaction done on the colonies of R. solanacearum isolates showed pink small rod-shaped cells under the microscope at ×100 magnification (Table 1). These Gram staining reaction observations are in line with those reported by Mutimawurugo et al. (2019) where all isolated R. solanacearum from three regions in Rwanda were Gram-negative short rods. This is because R. solanacearum is a Gram-negative rod-shaped bacterium and that all plant pathogenic bacteria are usually Gram-negative except Streptomyces and Clavibacter [23,36]. There was a formation of viscous thread following KOH tests on all the isolates. The observations conform to that reported by Teli et al. (2018) where the formation of mucoid or slime thread was observed when the bacterial suspension of R. solanacearum was raised from a glass slide containing 3% KOH [9]. The formation of viscous, mucoid, or slime thread is due to the outer membrane of Gram-negative bacteria being readily disrupted when exposed to 3% KOH releasing the viscous DNA [23]. The potassium hydroxide solubility test is an important test for confirmation of the Gram-negative test and helps in differentiating R. solanacearum from other wilt-causing microorganisms such as Fusarium [22].
The catalase test showed that all the isolates were able to produce gas bubbles when they were mixed with a drop of 3% hydrogen peroxide hence were considered as catalase positive. These findings conform to those found by Khasabulli et al. (2017), whereby R. solanacearum isolated from the Maseno region was catalase-positive [23]. The gas production mixed with hydrogen peroxide is due to the ability of R. solanacearum to produce catalase enzyme. Catalase enzyme protects the bacteria from the toxic effects of hydrogen peroxide by catalyzing its breakdown into water and oxygen [37]. The production of gas bubbles also shows that the bacteria are aerobic or facultative anaerobe. All isolates of R. solanacearum were able to produce gas from glucose after 24 h of incubation hence was considered positive for the gas test. This was also observed by Khasabulli et al. (2017), when they experimented on R. solanacearum, they found that R. solanacearum was able to produce gas within 18 h of incubation. The isolates under investigation were not able to hydrolyze starch. This was a result of the absence of clear zones on the agar after incubation, confirming that the isolates were for R. solanacearum. The findings were similar to the results of the studies conducted by Sharma and Singh (2019) and Bawari and Narendrappa (2019) who found that R. solanacearum was unable to hydrolyze starch [22,24].
Based on the molecular characterization of the samples, it was clear that the bands observed were in the range of 372 bp, confirming that the sample was R. solanacearum ( Figure 2). The findings are concurring with that of Paudel et al. (2020) who observed that R. solanacearum has four distinct phylotypes. Phylotype I generates 144 bp for primers Nmult21:1F/Nmult22: RR, phylotype II generates 372 bp for primers Nmult21:2F/Nmult22: RR, phylotype III generates 91 bp for primers Nmult23: AF/Nmult22: RR phylotype IV generates 213 bp for primers Nmult22:InF/Nmult22:RR [2,38]. Based on phylotype groups, the R. solanacearum species complex is divided into subspecies for instance phylotype I and phylotype III are classified as R. pseudosolanacearum phylotype II as R. solanacearum and phylotype IV as Ralstonia syzygii [39]. Therefore, the findings of this study confirm that phylotype II, which is R. solanacearum species is present in the JKUAT farm.

Pathogenicity Test
The R. solanacearum was confirmed to cause bacterial wilt in tomato plants. Bacterial wilt symptoms including yellowing of the younger lower leaves, dropping of the leaves, and wilting of the plants' leaves and stem during daytime were observed two weeks after transplanting and inoculation (Figure 3). One month later, there was the formation of brown discoloration of vascular bundles in the stem above the soil layer. Additionally, the infected plants showed milky white substance streaming from the cross-sectional cuttings of the stem a sign of bacteria in the vascular tissues [23]. Diameters of zones of inhibitions produced by BSF-based pupal exuviae chitosan and commercial chitosan showed no statistically significant differences. However, there were statistically significant differences in the antibacterial activities against the R. solanacearum among the various treatments (Table 2)

Pathogenicity Test
The R. solanacearum was confirmed to cause bacterial wilt in tomato plants. Bacterial wilt symptoms including yellowing of the younger lower leaves, dropping of the leaves, and wilting of the plants' leaves and stem during daytime were observed two weeks after transplanting and inoculation (Figure 3). One month later, there was the formation of brown discoloration of vascular bundles in the stem above the soil layer. Additionally, the infected plants showed milky white substance streaming from the cross-sectional cuttings of the stem a sign of bacteria in the vascular tissues [23].

Pathogenicity Test
The R. solanacearum was confirmed to cause bacterial wilt in tomato plants. Bacterial wilt symptoms including yellowing of the younger lower leaves, dropping of the leaves, and wilting of the plants' leaves and stem during daytime were observed two weeks after transplanting and inoculation ( Figure 3). One month later, there was the formation of brown discoloration of vascular bundles in the stem above the soil layer. Additionally, the infected plants showed milky white substance streaming from the cross-sectional cuttings of the stem a sign of bacteria in the vascular tissues [23]. Diameters of zones of inhibitions produced by BSF-based pupal exuviae chitosan and commercial chitosan showed no statistically significant differences. However, there were statistically significant differences in the antibacterial activities against the R. solanacearum among the various treatments (Table 2). a b c Diameters of zones of inhibitions produced by BSF-based pupal exuviae chitosan and commercial chitosan showed no statistically significant differences. However, there were statistically significant differences in the antibacterial activities against the R. solanacearum among the various treatments (Table 2). The exact mechanisms of antimicrobial activities of BSF-based pupal exuviae chitosan are not well known. However, there are some proposed modes of action reported in previous studies. The most known mechanism involved in antimicrobial activities is attributed to the presence of amino groups on the chitosan structure that causes electrostatic interaction between their positive charges and negative charges of lipopolysaccharide on the bacterial cell membrane [40]. The electrostatic interaction has been known to disrupt the cell wall and cell membrane thus interfering with its permeability. Another postulate is that chitosan anionic charges (amino group) bind to the negatively charged phosphate groups on DNA and amino acids of the proteins thus inactivating or inhibiting messenger RNA (mRNA) and protein synthesis [41]. Chitosan can also inhibit bacterial growth by chelating essential nutrients and metals [42].
Tomato plants treated with BSF-based pupal exuviae chitin and chitosan showed a reduction in disease incidence by 30.31% and 34.95%, respectively, whereas the disease severity was reduced by 22.57% and 23.66%, respectively, compared to the control (Table 3). These findings are in agreement with those reported by Algam et al. (2016) where they revealed the application of chitosan from shrimps through soil drenching reduced wilting incidence by 72% in tomatoes. Similarly, chitin and chitosan-based polymers showed a reduction in disease severity in cabbage and strawberry plants after being challenged with Alternaria brassicicola and Colletotrichum fructicola in a study carried out by Parada et al. (2018), reduced incidence of Fusarium wilt disease in tomato plants (Malerba & Cerana, 2018), and enhanced defense against Meloidogyne javanica in tomato plants [14,[43][44][45]. Tomato plants uninoculated with R. solanacearum did not show wilting symptoms (Figure 4). There was no significant difference in bacterial wilt disease incidence and severity between BSF-based pupal exuviae chitin and chitosan.
Microorganisms 2021, 9, x FOR PEER REVIEW 10 [14,[43][44][45]. Tomato plants uninoculated with R. solanacearum did not show wilting s toms ( Figure 4). There was no significant difference in bacterial wilt disease incidenc severity between BSF-based pupal exuviae chitin and chitosan. The reduction of wilting disease incidence and severity by BSF-based pupal ex chitin and chitosan is attributed to proposed mechanisms that include direct antibac activity through cell lysis [41], and the formation of a mechanical barrier that pr against pathogens [46]. They can trigger plant defense responses and activate dif pathways which increase the plant's resistance to diseases [12]. In certain instances can stimulate hypersensitive responses in plants primarily around the infection sit results in programmed cell death. The hypersensitive response can be followed by temic responses that include the synthesis and build-up of pathogenesis-related pro phytoalexins, and phenolic compounds. The systemic response can also modulate t tivity of key enzymes such as chitinase, catalase, and superoxide dismutase peroxide are involved in metabolic pathways in the defense response [41]. Chitosan showed results than chitin which may have been caused by the deacetylation and solubil chitosan. Recent studies have shown chitosan's ability to increase photosynthesis, tolerance to stress, and expression of defense genes which helps the plant to better infection [47]

Conclusions
The early detection of R. solanacearum belonging to phylotype II is essential fo venting its introduction into new areas due to its ability to survive for prolonged pe in soil, water, and plant materials. The avoidance of tomato losses due to this path would significantly contribute to increased crop production in the country. Alth many researchers have managed bacterial wilt with biological, physical, and che methods and/or with cultural practices; this study provides the first evidence on th ciency of BSF-based pupal exuviae chitin and chitosan antimicrobial activities and p tial to protect against R. solanacearum in tomato plants. The remarkable reduction o terial wilt disease incidence by 30.31% and 34.95% in cocopeat amended with chiti chitosan, respectively, isolated from BSF pupal exuviae demonstrated these shells a attractive renewable raw material for the recovery of valuable product with prom ability as a new type of eco-friendly control measure against bacterial wilt, R. solanace From the findings of this study, chitosan showed better results than chitin hence u chitosan is the best approach. Further studies should explore the effects of direct ap tion of the BSF pupal exuviae on R. solanacearum in soil and their integration into grated pest management strategies that involve multiple components to manage bac a b c The reduction of wilting disease incidence and severity by BSF-based pupal exuviae chitin and chitosan is attributed to proposed mechanisms that include direct antibacterial activity through cell lysis [41], and the formation of a mechanical barrier that protects against pathogens [46]. They can trigger plant defense responses and activate different pathways which increase the plant's resistance to diseases [12]. In certain instances, they can stimulate hypersensitive responses in plants primarily around the infection site that results in programmed cell death. The hypersensitive response can be followed by systemic responses that include the synthesis and build-up of pathogenesis-related proteins, phytoalexins, and phenolic compounds. The systemic response can also modulate the activity of key enzymes such as chitinase, catalase, and superoxide dismutase peroxides that are involved in metabolic pathways in the defense response [41]. Chitosan showed better results than chitin which may have been caused by the deacetylation and solubility of chitosan. Recent studies have shown chitosan's ability to increase photosynthesis, plant tolerance to stress, and expression of defense genes which helps the plant to better fight infection [47].

Conclusions
The early detection of R. solanacearum belonging to phylotype II is essential for preventing its introduction into new areas due to its ability to survive for prolonged periods in soil, water, and plant materials. The avoidance of tomato losses due to this pathogen would significantly contribute to increased crop production in the country. Although, many researchers have managed bacterial wilt with biological, physical, and chemical methods and/or with cultural practices; this study provides the first evidence on the efficiency of BSF-based pupal exuviae chitin and chitosan antimicrobial activities and potential to protect against R. solanacearum in tomato plants. The remarkable reduction of bacterial wilt disease incidence by 30.31% and 34.95% in cocopeat amended with chitin and chitosan, respectively, isolated from BSF pupal exuviae demonstrated these shells are an attractive renewable raw material for the recovery of valuable product with promising ability as a new type of eco-friendly control measure against bacterial wilt, R. solanacearum. From the findings of this study, chitosan showed better results than chitin hence use of chitosan is the best approach. Further studies should explore the effects of direct application of the BSF pupal exuviae on R. solanacearum in soil and their integration into integrated pest management strategies that involve multiple components to manage bacterial wilt diseases, thus significantly contributing to increased tomato production worldwide. Our primary goal is to contribute to environmentally safe, sustainable, and increased tomato production for income and food security. However, research attention to cost-benefit analyses is indispensable in the short, middle, and long term. The funders had no role in the study design, the data collection and analysis, the decision to publish, or the preparation of the manuscript; therefore, the views expressed herein do not necessarily reflect the official opinion of the donors.
Institutional Review Board Statement: Not applicable.

Informed Consent Statement: Not applicable.
Data Availability Statement: Not applicable.