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Article

Role of TRP Channels in Shaping the Gut Microbiome

1
Department of Internal Medicine-Molecular Medicine, Wake Forest School of Medicine, Winston-Salem, NC 27101, USA
2
Department of Molecular Biomedical Sciences, NC State Veterinary Medicine, Raleigh, NC 27606, USA
3
Department of Cancer Biology, Wake Forest School of Medicine, Winston-Salem, NC 27157, USA
4
Department of Microbiology and Immunology, Wake Forest School of Medicine, Winston-Salem, NC 27101, USA
*
Author to whom correspondence should be addressed.
Pathogens 2020, 9(9), 753; https://doi.org/10.3390/pathogens9090753
Received: 26 July 2020 / Revised: 29 August 2020 / Accepted: 9 September 2020 / Published: 16 September 2020
(This article belongs to the Section Animal Pathogens)
Transient receptor potential (TRP) channel family proteins are sensors for pain, which sense a variety of thermal and noxious chemicals. Sensory neurons innervating the gut abundantly express TRPA1 and TRPV1 channels and are in close proximity of gut microbes. Emerging evidence indicates a bi-directional gut–brain cross-talk in several entero-neuronal pathologies; however, the direct evidence of TRP channels interacting with gut microbial populations is lacking. Herein, we examine whether and how the knockout (KO) of TRPA1 and TRPV1 channels individually or combined TRPA1/V1 double-knockout (dKO) impacts the gut microbiome in mice. We detect distinct microbiome clusters among the three KO mouse models versus wild-type (WT) mice. All three TRP-KO models have reduced microbial diversity, harbor higher abundance of Bacteroidetes, and a reduced proportion of Firmicutes. Specifically distinct arrays in the KO models are determined mainly by S24-7, Bacteroidaceae, Clostridiales, Prevotellaceae, Helicobacteriaceae, Rikenellaceae, and Ruminococcaceae. A1KO mice have lower Prevotella, Desulfovibrio, Bacteroides, Helicobacter and higher Rikenellaceae and Tenericutes; V1KO mice demonstrate higher Ruminococcaceae, Lachnospiraceae, Ruminococcus, Desulfovibrio and Mucispirillum; and A1V1dKO mice exhibit higher Bacteroidetes, Bacteroides and S24-7 and lower Firmicutes, Ruminococcaceae, Oscillospira, Lactobacillus and Sutterella abundance. Furthermore, the abundance of taxa involved in biosynthesis of lipids and primary and secondary bile acids is higher while that of fatty acid biosynthesis-associated taxa is lower in all KO groups. To our knowledge, this is the first study demonstrating distinct gut microbiome signatures in TRPA1, V1 and dKO models and should facilitate prospective studies exploring novel diagnostic/ therapeutic modalities regarding the pathophysiology of TRP channel proteins. View Full-Text
Keywords: intestinal microflora; microbiota; pain; transient receptor potential; TRP channels; TRPA1; TRPV1 intestinal microflora; microbiota; pain; transient receptor potential; TRP channels; TRPA1; TRPV1
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MDPI and ACS Style

Nagpal, R.; Mishra, S.K.; Deep, G.; Yadav, H. Role of TRP Channels in Shaping the Gut Microbiome. Pathogens 2020, 9, 753. https://doi.org/10.3390/pathogens9090753

AMA Style

Nagpal R, Mishra SK, Deep G, Yadav H. Role of TRP Channels in Shaping the Gut Microbiome. Pathogens. 2020; 9(9):753. https://doi.org/10.3390/pathogens9090753

Chicago/Turabian Style

Nagpal, Ravinder, Santosh K. Mishra, Gagan Deep, and Hariom Yadav. 2020. "Role of TRP Channels in Shaping the Gut Microbiome" Pathogens 9, no. 9: 753. https://doi.org/10.3390/pathogens9090753

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