Assessment of the Antimicrobial Potentiality and Functionality of Lactobacillus plantarum Strains Isolated from the Conventional Inner Mongolian Fermented Cheese Against Foodborne Pathogens

Lactobacillus plantarum are amongst the diversified lactic acid bacteria (LAB) species which are being utilized abundantly in the food industry. Numerous L. plantarum strains have been reported to produce several antimicrobial compounds. Diacetyl, hydrogen peroxide, organic acids, as well as bacteriocins can also be exemplified by a variable spectrum of actions. The current study was intended to conduct the screening and characterization of antimicrobial prospective of L. plantarum from traditional Inner Mongolian fermented hard cheese. Foodborne pathogens, Salmonella typhimurium, Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus, were examined by using the Oxford cup technique and the mixed culture inhibition assays. The resulting analyses disclosed that L. plantarum KLDS1.0344 indicated broad antimicrobial spectrum against all selected pathogens as compared to other LAB used in this study. Additionally, the decrement of the pathogen population was observed up to 3.47 logs in mixed culture inhibition assays. L. plantarum KLDS 1.0344 acid production was recorded up to 71.8 ± 3.59 °D in mixed culture while antimicrobial particles released in cell free supernatants demonstrated bacteriocin-like characteristics showing substantial pH stability (2.0–6.0), proteolytic enzyme reduced the antibacterial activity (15.2 ± 0.6 mm–20.4 ± 0.8 mm), heat stability (20 min at 120 °C) against selected pathogens. Moreover, the spectrum range of antimicrobial peptides after the partial purification was decreased as compared to the crude bacteriocin-like compound. The SDS-PAGE analysis showed the molecular weight range of partially purified bacteriocin from 12 to 45 kDa. After analyzing the obtained data from the current experimentation showed that the capability of L. plantarum KLDS 1.0344 to oppose the pathogen growth in vitro relies on the occurrence of organic acids along with bacteriocin-like compounds proving L. plantarum KLDS 1.0344 as a potentially appropriate candidate as an alternative bio-control agent against foodborne pathogens.


Determination of Inhibitory Substances of L. plantarum KLDS 1.0344 and Evaluate the Effect of Protease Enzymes on Their Activity
The technological applicability of LAB characteristically grounded on the study of acidifying capability. In pure form, L. plantarum KLDS 1.0344 produced the highest levels of acidity 50.8 ± 2.94 • D in 44 h. In the mixed culture of L. plantarum with S. typhimurium, L. monocytogenes, S. aureus and E. coli, a high acidity (71.8 ± 3.59 • D) was witnessed at 44 h (significant p < 0.05). Kinetics of the pH progress ( Figure 1) exhibited that L. plantarum reduced pH up to 4.7 ± 0.02 in 44 h, while, in the mixed culture of L. plantarum and S. typhimurium, L. monocytogenes, S. aureus and E. coli was 4.3 ± 0.01. Insofar as, an antibacterial behavior of bacteriocin-like composite from L. plantarum KLDS 1.0344 for all selected pathogens was significantly declined after treating with several different proteolytic enzymes such as, pepsin treated with 12.7 ± 0.5 mm for L. monocytogenes, 18.5 ± 0.8 mm for S. aureus, 16.3 ± 0.7 mm for S. typhimurium, and 15.6 ± 0.6 mm for E. coli., From the results it was confirmed that the minimum inhibition zone was observed in bromelain enzyme case, 12.5 ± 0.5 mm for L. monocytogenes, 18.2 ± 0.7 mm for S. aureus, 16.0 ± 0.6 mm for S. typhimurium, and 15.3 ± 0.6 mm for E. coli. While the lowest decrease in inhibition zones were observed for protease treatment (Table 3). With every proteolytic enzyme treatment the antimicrobial activity clearly decreased, which exhibited that the antimicrobial compounds having a protein nature.

Partial Purification, Molecular Weight, Thermal and pH Stability of Bacteriocin
In present research work, bacteriocins-like compounds, produced by L. plantarum (KLDS 1.0344), were partly cleaned by precipitation and dialysis of ammonium sulfate. Antimicrobial potential of bacteriocin was assessed against four pathogens, such as S. aureus, S. typhimurium, E. coli and L. monocytogenes. Inhibition zones of four pathogens were ominously dissimilar from each other after different treatments. Bacteriocin produced by L. plantarum KLDS 1.0344 after partial purification displayed ultimate antibacterial activity (16.0 ± 0.65-21.4 ± 0.83 mm) against the contestant pathogens as compared to the crude form (significantly p < 0.05) ( Figure 2). The obtained partially purified bacteriocin from L. plantarum KLDS 1.0344 was treated at different levels of pH to assess its antibacterial potentiality against pathogens ( Table 4). The antibacterial potentiality of peptides contrary to all pathogens was lessened with augmented pH values. No antibacterial activity was observed beyond a pH of 6.0. Hence, the pH range of antibacterial activities of obtained bacteriocin from L. plantarum KLDS1.0344 was pH 2-6. Table 5 also demonstrates that the thermal stability of bacteriocin resembling compounds were successively steady after subsequent treatments at 120 • C for 20 min retained inhibition activity of bacteriocin-like L. plantarum KLDS 1.0344 against pathogens L. monocytogenes (85.0 ± 0.7%), S. aureus (86.5 ± 0.3%), S. typhimurium (83.0 ± 0.0%), E. coli O157:H7 (81.0 ± 0.2%). According to the SDS-PAGE analysis, bacteriocins-like are small polypeptides with a molecular weight in the range of 12, 35 and 45 kDa (Figure 3). monocytogenes. Inhibition zones of four pathogens were ominously dissimilar from each other after different treatments. Bacteriocin produced by L. plantarum KLDS 1.0344 after partial purification displayed ultimate antibacterial activity (16.0 ± 0.65-21.4 ± 0.83 mm) against the contestant pathogens as compared to the crude form (significantly p < 0.05) ( Figure 2). The obtained partially purified bacteriocin from L. plantarum KLDS 1.0344 was treated at different levels of pH to assess its antibacterial potentiality against pathogens ( Table 4). The antibacterial potentiality of peptides contrary to all pathogens was lessened with augmented pH values. No antibacterial activity was observed beyond a pH of 6.0. Hence, the pH range of antibacterial activities of obtained bacteriocin from L. plantarum KLDS1.0344 was pH 2-6. Table 5 also demonstrates that the thermal stability of bacteriocin resembling compounds were successively steady after subsequent treatments at 120 °C for 20 min retained inhibition activity of bacteriocin-like L. plantarum KLDS 1.0344 against pathogens L. monocytogenes (85.0 ± 0.7%), S. aureus (86.5 ± 0.3%), S. typhimurium (83.0 ± 0.0%), E. coli O157:H7 (81.0 ± 0.2%). According to the SDS-PAGE analysis, bacteriocins-like are small polypeptides with a molecular weight in the range of 12, 35 and 45 kDa (Figure 3).

Mixed Culture Inhibition Assay
While exploring the antimicrobial effects of L. plantarum KLDS 1.0344 on E. coli, S. typhimurium, S. aureus and L. monocytogenes, we executed the mixed culture inhibition assay and the results are shown in Figure 4. Observations revealed that in all groups pH of the cultures displayed evidential (p < 0.05) decline. Viable counts of L. plantarum with all pathogens in monoculture co-cultured and diffusion chamber groups are noticeably different (p < 0.05) among the groups with an increasing trend. Figure 4A-D) exhibits the growth of L. plantarum KLDS 1.0344 (maximum population of 8.8 log CFU per mL) was comparable to that attained in monoculture.   Whereas, decline in the number of viable cells of the E. coli O157:H7, S. typhimurium, L. monocytogenes and, S. aureus (ATCC 19115) in diffusion chamber, as well as in co-cultured groups ranged from 2.56 to 3.47 logs, were highly significantly different from the counts of all pathogen in monoculture (p < 0.001). Viable counts of pathogens decreased in diffusion chamber and co-cultured groups, whilst increased in monoculture group. Besides, the viable count of pathogens in the diffusion chamber group reduced up to 0 logs per ml after 20 h, while flatly declined to in co-culture group. Withal, in three groups the pH values of BHI were not significantly dissimilar (p > 0.05). Furthermore, the results from diffusion chamber culture and co-culture of L. plantarum KLDS 1.0344 with E. coli, S. typhimurium, S. aureus and L. monocytogenes, signifying the participation of a secreted inhibitory molecule from L. plantarum KLDS 1.0344, as well as, bacteria itself. However, only the antibacterial substances secreted by L. plantarum KLDS 1.0344 in the diffusion chamber group were involved in the inhibition of all pathogens. Bacterial cells were physically parted in the diffusion chamber group, while the diffusion of extracellular compounds and nutrients through the filter was probable. Whilst, contrasting the diffusion chamber group, bacterial cells could also interact with co-culture group pathogens. It might be stated that, the inhibitory effect of L. plantarum KLDS 1.0344 against the given foodborne pathogenic strains is due to the contact-dependent inhibition (CDI) mechanism.

Discussion
In the present study, L. plantarum KLDS 1.0344 (Tables 1 and 2) displayed a stronger antimicrobial activity against the two pathogens (Gram positive) i.e., S. aureus and L. monocytogenes. And likewise, exhibited antimicrobial potentiality against two Gram negative pathogenic strains, i.e., E. coli and S. typhimurium.
Employment of LAB has been of significant interest to constrain the pathogens (in vitro). Presently, numerous Lactococcus and Lactobacillus species have been assessed for their executability to impede the progression of Gram negative and Gram positive infectious agents in different prototypical organisms [46]. The bactericidal effect of protease sensitive bacteriocins of the LAB has been mostly shown to obstruct the activities of Gram negative pathogens [47,48]. Conversely, the hostility of LAB against Gram-negative pathogens are thought to be owing to the formation of hydrogen peroxide and organic acids [49].
LAB comprising numerous L. plantarum strains had been investigated to yield varied antimicrobial agents, for instance, hydrogen peroxide, organic acids, bacteriocins, diacetyl and antimicrobial peptides, owing to a versatile range of mechanisms [17,50,51]. Some LAB, like, L. plantarum, display adverse effect towards spoilage and pathogenic microbes. This antagonistic attitude has been frequently attributed to produce (organic) lactic and phenyl lactic acids [12][13][14]52]. On the other hand, the production of (bacteriocins or bacteriocin-like) compounds has been found to explicate the antimicrobial potency applied by lactobacilli [15,16,53].
Numerous studies ascertained that LAB has the capability to yield antibacterial semantics counting, inhibitory enzymes, organic acids, diacetyl, bacteriocins, and hydrogen peroxide to constrain the growth of pathogenic strains up to a very wide range [54,55]. Loss of antibacterial activity of L. plantarum KLDS 1.0344 was found subsequently treating with pepsin, demonstrating that bacteriocin-identical compound was the peptide. After treating with protein digested enzymes like pronase, pepsin, bromelain, protease, ficin and α-chymotrypsin. Decreasing antimicrobial activity unveiled that the bacteriocin was peptide in nature (Table 3). Bacteriocins are the heterogeneously diverse group of proteins and antibacterial peptides that differ in their mode of action, the spectrum of activity, genetic origin, molecular mass and biochemical properties [56]. Some other researchers investigated the bactericidal influence might be due to the organic acids alone or in a blend with bacteriocins peptides [57].  [66] reported that the bacteriocins like bacST216Ch and bacST202Ch, excreted by the L. plantarum strains sequestered from Chourico and Beloura, restrained the growth of certain meat putrefying Gram negative and positive bacteria. L. plantarum B0105 isolated from the conventionally fermented mustard of Taiwan, pragmatic to excrete bacteriocin that inhibited the Streptococcus mutans [67]. L. plantarum isolated from goat feta cheese exhibited bactericidal impact to resist Listeria monocytogenes [65].
Numerous lactococci and lactobacilli species are nominated to be inhibitory against S. aureus and E. coli in vivo and in vitro [36,60,[68][69][70][71][72][73][74]. Lactic acid bacteria strains like Lactobacillus plantarum, Lactobacillus paracasei and four strains of Lactobacillus fermentum, not only, showed the better probiotic potential, but also, decreased the proliferation of enteropathogenic bacteria which were isolated from healthy infant feces, such as, Shigella flexneri Yersinia enterocolitica, Salmonella enteritidis and Shigella sonnei [75]. These Lactobacillus strains have been proved to be useful to treat diarrhea [76].
The complete disappearance of antibacterial properties of bacteriocin obtained from L. plantarum KLDS 1.0344 was detected against S. aureus, S. typhimurium, E. coli. and L. monocytogenes when pH was adjusted to 7 (Table 4). Lin et al. (2008) [57] also disclosed that the cultures of LAP5 cells neutralized to 7.0 pH, the alienating properties against Salmonella indicated non inhibitory potential. Alike findings testified that at pH 5.0, acidocin B produced by L. acidophilus retained 50% activity [77]. Main categories of LAB tailored bacteriocins consisted of lantibiotics, large heat-stable proteins and complex peptides, while heat stable property of bacteriocin compounds increased their applications [56,77]. In our present research work, the bacteriocin from L. plantarum KLDS 1.0344 has been found considerably heat and pH tolerant and potent bio preservative agent likewise. These potentials could make it useful natural food additive in food applications, which are at present, the matter of broad and extensive research. Searching the new bacteriocins encompassing a panoramic spectrum of antimicrobial activity are being investigated by some researchers.
Furthermore, results from Figure 4A-D) exhibited the viable count of four pathogens declined at 3.47 logs in the diffusion chamber group and 2.56 logs in co-culture group. These findings were analogous to the results of Atassi et al. (2006) [78] study, which disclosed that in viable cells of bacteria, the L. helveticus KS300 reduced pathogenic E. coli C1845, S. typhimurium SL1344 and IH11128 with a reduction of 2.0-5.5 logs. We should essentially emphasize that the unusual factors might be prospective for these outcomes and additional positive aspects could more decrease pathogenic progression. Former investigations on co-culture reticence provided an antimicrobial theory known as contact-dependent inhibition (CDI) mechanism [79][80][81]. In the present study it has been shown that the antimicrobial potential of the L. plantarum was associated with bacteria itself and bactericidal activity of the diffusion chamber group was compromising than the co-culture group. It is expected that, the antimicrobial action of bacteria itself could be correlated to the previously stated CDI mechanism. This mechanism could be illuminated by the interchange of information amongst the bacteria. Such ransfer of information encompassed, secretion systems, conjugation, allolysis, and nanotubes and contact-dependent inhibition [79].
Type IV and VI secretion pathways were ascertained in (Gram negative) bacteria. They assist the transportation of molecules, proteins or DNA straight into prokaryote cells from the bacterial cytoplasm. Conjugation is a straight allocation of genetic material amongst the microbial cells by a bridge-like connection or through smooth cell-to-cell contact [82]. Apart from these pathways, several researchers also verified that certain Escherichia coli strains might cause CDI of other E. coli strains [81,83,84]. CDI mechanism was identified among proteo-bacteria, but did not demonstrate experimentally in (Gram positive) bacteria [85].
According to Jabbari et al. (2017) [86], during co-cultivation with the pathogens and separate cultivation at 37 ± 1 • C, L. plantarum kept the high concentration of viable cells. L. plantarum reduced the concentration of viable cells of E. coli ATCC 25922, Klebsiella pneumonia and Salmonella spp. The researcher has described thatthe inhibition of the growth of pathogens was attributable to generate other organic acids and lactic acid, which acidified the medium and altered the conditions for the growth of the pathogens.

Bacterial Strains and Growth Conditions
L. plantarum KLDS 1.0317, 1.0318, 1.0344, 1.0386, 1.0628, 1.0985, 1.0986 and other Lactobacilli utilized for antimicrobial spectrum were sequestrated from conventional cheese in Inner Mongolia and stored in Key Lab Dairy Science, Ministry of Education, China. 16S rDNA sequence analysis was used for the identification of these strains and these were anaerobically incubated in modified de Man, Rogosa, and Sharpe (mMRS) broth [87] at 37 • C. Four pathogenic strains, namely, Escherichia coli O157:H7 (ATCC 43889), Salmonella typhimurium (ATCC 14028), Listeria monocytogenes (ATCC 19115) and Staphylococcus aureus (ATCC 25923) were procured from entry-exit IQB (Inspection and Quarantine Bureau), Hubei province of China. These pathogenic strains were then aerobically incubated in BHI (Brain Heart Infusion broth, Qingdao Hope Biotechnology Co., Ltd. Shandong, China) at 37 • C and were utilized as indicator bacteria for the antimicrobial assays.
The Oxford cup technique for the antimicrobial potentiality of L. plantarum KLDS strains against foodborne pathogens was explored in accordance to Wang et al. 2007Wang et al. :2009 and Zhai et al. (2015) [90], with some adjustments. Initially, 12 mL of agar medium (1.5% w/v) was poured into the plate and was solidified. Formerly, in the stationary phase, 1% of indicator strain was injected into 12 mL of apposite BHI agar 1.2% (w/v), at 45 • C. Then, the mixtures were moved onto agar media and permitted to harden. Then, three sterilized Oxford Cups were taken. These cups were placed and pressed slightly on BHI agar surface, to degas the interspace between agar surface and cups. Later on, 200 µL of CFS and the same volume of culture dribbled into two cups, respectively. Then, as control, sterile water (200 µL) delivered into the third cup. Plates were piled into incubator by providing aerobic conditions at 37 • C for 24 h or 48 h and, antimicrobial activity was witnessed around the Oxford Cups, close to growth-free inhibition zones. Inhibition zones computed from the edges of cups in mm. The current trial was performed in triplicate.

Antibacterial Ambit of L. plantarum KLDS 1.0344
The antibacterial ambit of L. plantarum KLDS 1.0344 was evaluated in comparison to eleven indicator strains enclosing Lactobacilli and foodborne pathogens by the use of Oxford Cups. Primarily, 12 mL of agar medium (1.5% (w/v) was dispensed into the plate and uphold till solidified. In the stationary phase, 1% of indicator strain was injected into 12 mL of apposite agar medium 1.2% (w/v), at 45 • C. Formerly on agar medium the mix transferred and permitted to congeal. Then, three sterilized Oxford Cups were taken. These cups were placed and pressed slightly on BHI agar surface, to degas the interspace between agar surface and cups. Later on, 200 µL of CFS and the same volume of culture dribbled into two cups, respectively. Then, as control, sterile water (200 µL) delivered into the third cup. Plates were piled into incubator by providing aerobic conditions at 37 • C for 24 h or 48 h and, antimicrobial activity was witnessed around the Oxford Cups, close to growth-free inhibition zones. Inhibition zones computed from the edges of cups in mm. The current trial was performed in triplicate.
The plates were put in incubator providing aerobic conditions at 37 • C for 24 h or 48 h and around the Oxford Cups. Inhibition zones computed from the edges of cups in mm. The incumbent experiment conceded in triplicate.

Quantification of Acid Production of L. plantarum KLDS 1.0344
To determine the acid production capability of L. plantarum KLDS 1.0344, a deduction of 10 mL of pure culture L. plantarum KLDS1.0344 and mixed culture with E. coli, L. monocytogenes, S. typhimurium and S. aureus was individually transferred in a 100 mL conical flask and 5 droplets of phenolphthalein indicator (2 mg/mL in ethanol 60 • ) were imparted. NaOH 1/9N was used to neutralize acidity until the appearance of a persistent pink color. Titrating solution volume was deliberated to prefigure the production of acidity, assessed in dornic degree and a PHS-3C electrode pH meter (METTLER TOLEDO, Switzerland) was used to measure the pH of the culture after every 4 h. Each experiment was replicated thrice [60].

Mixed Culture Inhibition Assay
Antimicrobial potentiality against E. coli, S. aureus, S. typhimurium and L. monocytogenes cells was performed by using a mixed culture method. Equal volumes of L. plantarum KLDS1.0344 (10 6 CFU per mL) and 10 3 CFU per mL of each pathogen were co-cultured into a diffusion chamber which was split with a 0.22 µm size of the filter in their respective cultivating medium as described by Saraoui et al. (2016) [79] and, incubation was executed at 37 • C for 24 h. 1% of both L. plantarum KLDS 1.0344 and pathogens 10 6 CFU per mL and 10 3 CFU per mL, respectively were mono-cultured into mMRS and BHI broth used as a control medium. Every 4 h, all the cultures were dispersed onto mMRS agar and BHI plates with proper dilutions, and 20h incubation was done at 37 • C. Colonies of L. plantarum KLDS 1.0344 and pathogens were computed. PHS-3C electrode pH meter (METTLER TOLEDO, Switzerland) was used to measure the pH of above-mentioned cultures, after every 4 h. Every experiment was replicated thrice.

Antimicrobial Peptide Production
100 mL mMRS broth was inoculated with 2% L. plantarum KLDS 1.0344 (10 8 CFU per mL) and incubated at 37 • C for 24 h. Afterwards, culture centrifugation was performed at 10,000× g for 10 min at 4 • C. All supernatants were then filter-sterilized using a sterile porous filter (0.22 µm). 1N NaOH was used to neutralize the resulting CFS to pH 6.5 and catalase (5 mg per mL) was used for the elimination of the hydrogen peroxide inhibitory effect. Finally, neutralized CFS (bacteriocin-like and bacteriocin metabolites) of the L. plantarum were tested against four pathogenic bacteria (L. monocytogenes, S. aureus, E. coli O157:H7 and S. typhimurium) to evaluate the antibacterial activity [91].

Antimicrobial Fractional Refinement by Dialysis and Ammonium Sulfate Precipitation
20 mL of crude bacteriocin-like were taken and ammonium sulfate solution (60%) was incrementally dispensed on the sample in a glass beaker. A magnetic stirrer (85-2 Hangzhou instrument motor Co., Ltd., Hangzhou, China) was used to stir the mixture for 2 h. Centrifugation (at 10,000 rpm) of the solution was accomplished at 4 • C, for 20 min. The precipitate was re-suspended in 25 mL buffer of potassium phosphate (10 mM, pH 7.0), after discarding the supernatant. Dialysis membrane was cut into the desired length, softly squeezed and opened. A cutoff value of the dialysis membrane was less than 1200 Da. The membrane was closed by fastening with a thread from one end. Samples of bacteriocin-like peptides were decanted on the dialysis membrane and knotted on a glass rod. Then, it was retained half soaked in (10 mM) phosphate buffer solution in a beaker and gently stirred for 12-18 h with a magnetic stirrer. To confirm appropriate dialysis of protein, the buffer was changed for every 3 h during the mixing procedure. Membrane bag was collected after dialysis completion, and stored at 4 • C carefully. Finally, the determination of the antibacterial activity of the dialyzed bacteriocin was performed by agar well diffusion procedure [91] For the rectification of the antimicrobial elements of L. plantarum CFS were prepared by incubating CFS (1 h at 37 • C) with protease, pepsin, bromelain, pronase, α-chymotrypsin, and ficin. All enzymes were utilized in potassium phosphate buffer (10 mmol per L, pH 7.0) with a concentration of 5 mg per mL. Controls were sustained by using CSF in buffer without having enzymes. Residual antimicrobial inhibition of S. aureus, E. coli, L. monocytogenes and, S. typhimurium was assessed with the use of Oxford Cups. All experiments were replicated thrice [11].

Thermal Stability of Partially Purified Antimicrobial Compounds
Incubation of partially purified bacteriocin containing samples was carried out at respective temperatures of 80 • C, 100 • C, 120 • C and samples were taken at respective intervals of 20, 30 and 40 min. Samples were cooled to room temperature after heat treatment, and agar well diffusion test was done to assess the bactericidal of bacteriocin [92]. The Inhibition activity (%) was calculated as in Equation (1). (1) Inhibition zone diameter = mean value of the diameter of the treated sample (mm) Max. Inhibition zone diameter = mean value of diameter for control (mm)

pH Stability of Partially Purified Antimicrobial Compounds
The pH stability of partially purified bacteriocin was tested by gradual pH adjustment from 2-8, in stages of one pH unit using 1N NaOH or 1N HCl. The incubation of the samples was done for 1 h at 30 • C and agar well diffusion technique was utilized to admeasure the bactericidal activity of the (partially refined) bacteriocin in triplicate against L. monocytogenes, E. coli, S. typhimurium and S. aureus were used as indicators. Inhibition activity (%) was calculated by using Equation (1) [67].

Electrophoresis Analysis
The molecular weight of partially purified bacteriocin preparations was determined by SDS-PAGE (Sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The 30 µL sample was homogenized with 2% SDS and dithiothreitol (DTT) adding a little glycerin to increase the density and heating in boiling water for 5 min deal and then loaded onto gel, along with molecular weight marker mix like by using a Mini-Protean 4 cell system (Bio-Rad, Hercules, CA, USA), 100 V and 20 mA in the separation gel at pH 8.3. The gel was stained with 50 mL of (0.1 M) Coomassie brilliant blue R-250 for 4 h. The decolorizing solution was decolorized for 12 h until the electrophoresis band was clear. After the background was decolorized and cleaned, the gel imaging system was used for imaging and data acquisition [91].

Statistical Analyses
Entire analyses were completed in triplicate and the data was compiled. Tukey method was utilized by using the statistical program Statistics 8.1 (Analytical Software, SAS/STAT ® , Cary, NC, USA) for analysis of variance (ANOVA) and comparison of means. Statistically, the difference was pondered as significant when the p < 0.05. Standard errors and mean values were deliberated and demonstrated in charts as coordinate pairs with error bars.

Conclusions
This study explored that the L. plantarum KLDS 1.0344 possesses a substantial antimicrobial capability against miscellaneous pathogenic bacterial strains, including both the Gram negative and Gram positive types in vitro. Acid production, as well as the biosynthesis of bacteriocin-like compounds is anticipated as one of the mechanisms through which the antimicrobial activity is wielded by L. plantarum KLDS1.0344. Bacteriocin-like compounds cultivated from L. plantarum KLDS1.0344 displayed vehement antibacterial competency against foodborne pathogens, pH and heat stability and, sensitivity to proteolytic enzymes. The outcome of the present study provides information about the antimicrobial potentials of the KLDS strains and this information could be helpful to select the probiotics, starters and natural antimicrobial agents against foodborne pathogens in meat-based processed or partially processed food products (Hams, salami, sausages), milk based fermented (cheeses, kefir, yoghurt, sour cream) and non-fermented products (infant formula powders, ice creams, desserts). As in this modern era the consumers are aware of the concepts of food safety, the consumption of healthy and safer food is getting prime importance, so, on the basis of results obtained from the current study, it might be anticipated that these discoveries will intensify the use of L. palntarum in the milk and meat-based food processing industries to produce safe, healthy and longer shelf life food products.