Bioenergetics of Mycobacterium: An Emerging Landscape for Drug Discovery

Mycobacterium tuberculosis (Mtb) exhibits remarkable metabolic flexibility that enables it to survive a plethora of host environments during its life cycle. With the advent of bedaquiline for treatment of multidrug-resistant tuberculosis, oxidative phosphorylation has been validated as an important target and a vulnerable component of mycobacterial metabolism. Exploiting the dependence of Mtb on oxidative phosphorylation for energy production, several components of this pathway have been targeted for the development of new antimycobacterial agents. This includes targeting NADH dehydrogenase by phenothiazine derivatives, menaquinone biosynthesis by DG70 and other compounds, terminal oxidase by imidazopyridine amides and ATP synthase by diarylquinolines. Importantly, oxidative phosphorylation also plays a critical role in the survival of persisters. Thus, inhibitors of oxidative phosphorylation can synergize with frontline TB drugs to shorten the course of treatment. In this review, we discuss the oxidative phosphorylation pathway and development of its inhibitors in detail.


Introduction
Tuberculosis (TB) remains a leading cause of death worldwide, with an estimated 1.3 million mortalities in 2016. The synergy of human immunodeficiency virus (HIV)-TB co-infection has further aggravated this as a major public health problem. TB treatment is riddled with the use of multiple drugs for at least 6 months. Such lengthy treatment has resulted in the rise of multidrug resistant (MDR) and extremely drug resistant (XDR) strains of Mycobacterium tuberculosis (Mtb). Treatment of MDR TB requires administration of a multitude of second-line TB drugs for 18-24 months; this leads to cure rates of 60-70%. Of even more concern, cure rates for XDR-TB range from 40-50%. These low cure rates could be further confounded by other factors, such as co-infections and poor nutrition. Given these observations, new drugs with novel mechanisms of action are urgently required. Such innovative drugs could synergize with current treatment regimens to improve recovery rates and shorten treatment times. Extensive research efforts have been made in this direction, and the U.S. Food and Drug Administration has recently approved two new drugs. These drugs are bedaquiline (BDQ), a diarylquinoline ATP synthase inhibitor, and delamanid, a nitro-dihydro-imidazooxazole derivative that inhibits mycolic acid biosynthesis. Accelerated approval of these drugs has raised hope for a new regimen that could improve the outcome of treatment and reduce daily dose burden.
The myth that targeting Mtb bioenergetics would be a poor therapeutic strategy due to the presence of parallel and alternative metabolic pathways has been dispelled by the discovery of BDQ. Given the success of this compound, several research groups have focused on targeting oxidative Since cholesterol metabolism is important for mycobacterial survival within the host, the effect of exposure of Ro 48-8071 (an inhibitor of cholesterol biosynthesis) on mycobacterial growth was analyzed. This compound killed Mycobacterium bovis Bacillus Calmette Guerin (BCG) cells at low micromolar concentrations [52]. Further metabolic labelling experiments identified MK biosynthesis to be the target of Ro 48-8071. These findings were supported by the observation that Ro 48-8071 was capable of reducing oxygen consumption during growth. Further scrutiny led to the identification of MenA (Rv0534c) as the target of Ro 48-8071. The same group utilized a rational drug design approach to target MenA. They screened around 100 molecules, and established that an allylaminomethonone class of compounds are potent inhibitors of Mtb strains, including those which are drug-resistant [53]. This class of MenA inhibitors was also highly effective against hypoxia-induced drug-tolerant persisters [54]. Interestingly, aurachin RE, a new quinoline antibiotic isolated from Rhodococcus erythropolis JCM 6824 is highly effective against Gram-positive bacteria [55]. The aurachin RE molecules were modified to specifically target MenA, and validated as MenA inhibitors in functional in vitro assays. These compounds were low micromolar inhibitors of aerobic growth and hypoxic survival of Mtb [56]. Additionally, 7-methoxy-2-naphthol-based molecules have been utilized as lead structures for the development of non-traditional inhibitors of MenA of Mtb [57]. These efforts led to the development of bicyclic inhibitors that exert potent growthsuppressing activity against Mtb [57]  Since cholesterol metabolism is important for mycobacterial survival within the host, the effect of exposure of Ro 48-8071 (an inhibitor of cholesterol biosynthesis) on mycobacterial growth was analyzed. This compound killed Mycobacterium bovis Bacillus Calmette Guerin (BCG) cells at low micromolar concentrations [52]. Further metabolic labelling experiments identified MK biosynthesis to be the target of Ro 48-8071. These findings were supported by the observation that Ro 48-8071 was capable of reducing oxygen consumption during growth. Further scrutiny led to the identification of MenA (Rv0534c) as the target of Ro 48-8071. The same group utilized a rational drug design approach to target MenA. They screened around 100 molecules, and established that an allylaminomethonone class of compounds are potent inhibitors of Mtb strains, including those which are drug-resistant [53]. This class of MenA inhibitors was also highly effective against hypoxia-induced drug-tolerant persisters [54]. Interestingly, aurachin RE, a new quinoline antibiotic isolated from Rhodococcus erythropolis JCM 6824 is highly effective against Gram-positive bacteria [55]. The aurachin RE molecules were modified to specifically target MenA, and validated as MenA inhibitors in functional in vitro assays. These compounds were low micromolar inhibitors of aerobic growth and hypoxic survival of Mtb [56]. Additionally, 7-methoxy-2-naphthol-based molecules have been utilized as lead structures for the development of non-traditional inhibitors of MenA of Mtb [57]. These efforts led to the development of bicyclic inhibitors that exert potent growth-suppressing activity against Mtb [57].
High-density phenotypic profiling has identified menB (Rv0548c) as essential for mycobacterial growth [58]. MenB catalyzes the formation of 1,4-dihydroxy-2-naphthoyl-CoA from o-succinylbenzoyl CoA through a Claisen condensation reaction. Li and coworkers performed an HTS with more than 100,000 compounds, which identified a few hundred leads. Subsequent elaboration yielded multiple 1,4-benzoxazine compounds that were potent inhibitors of MenB activity, and consequently blocked mycobacterial growth [59]. These leads were then utilized for the development of 4-oxo-4-chlorophenylbutenoyl methyl ester derivatives with potent mycobactericidal activity against replicating and non-replicating Mtb [60].
MenE (Rv0542c) is the fifth enzyme in the MK biosynthesis pathway, and catalyzes the formation of OSB-CoA from O-succinyl-1-benzoate (OSB) through two mechanistic steps. The first is the ATP-dependent adenylation of OSB, and the second step is thioesterification with CoA. Given the structural and functional similarity of MenE with adenylate-forming enzymes, it is a promising target of acyl-AMP intermediates. Considering the mechanistic details, Tan and coworkers employed OSB-AMP analogues to inhibit MenE in Mtb [61]. The IC 50 of OSB-AMP analogues MeOSB-AMS, MeOSB-AMSN, and MeOSB-AVSN for MenE are in the low micromolar range [61]. Further development of these analogues resulted in the identification of OSB-AMS with an IC 50 for MenE in the nanomolar range; these compounds are competitive inhibitors of the binding of ATP and OSB [62]. Paradoxically, these inhibitors had poor mycobacterial growth inhibitory activity, perhaps due to their instability, and the OSB-AMP analogues were, therefore, further modified. This resulted in the development of OSB-AMS. Importantly, the difluoroindanediol analogue 11 of OSB-AMS was an effective mycobactericidal compound [63].
In an effort to identify inhibitors of oxidative phosphorylation, Alland and coworkers created a cell-based screen [64]. In this screen, the mWasabi reporter was fused to the promoter of the cydAB operon (coding for part of cytochrome bd oxidase) to monitor inhibition of respiration in the presence of 168 known inhibitors of mycobacterial growth. This led to the identification of DG70 as an inhibitor of bacterial respiration. DG70 is a biphenyl benzamide that kills both drug-sensitive and -resistant Mtb cells [64]. Interestingly, this novel inhibitor is highly specific against Mtb, and does not kill non-tuberculous mycobacteria (NTMs), Gram-positive, Gram-negative, or ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumonia, Acinetobacter baumannii, P. aeruginosa, and Enterobacter) pathogens. Sequencing of two DG70-resistant spontaneous mutant strains suggested the presence of a unique single nucleotide polymorphism (SNP) in menG. MenG (Rv0558, also known as MenH) catalyzes the conversion of demethylmenaquinol to menaquinol. Importantly MK4, an analogue of MK9, rescued the DG70-mediated killing of Mtb cells, thus validating that MK biosynthesis is the target of DG70. Importantly, DG70 synergizes with BDQ, isoniazid (INH), and PA824 to kill Mtb [64]. In summary, all these observations indicate that small molecule targeting of MK biosynthesis (summarized in Figure 1) is a valid therapeutic strategy.

Oxidation of Menaquinone: A Tale of Two Terminal Oxidases
Mycobacteria possess a branched ETC. During aerobic respiration, electrons are fed into the menaquinone (reduced/oxidized) pool of respiratory chains. Electrons are then transferred to oxygen through two branches, each of which possesses a terminal oxidase. One is cytochrome bd-type menaquinol oxidase (encoded by cydABDC), wherein electrons are transferred directly from MK (reduced) to oxygen. The other branch runs through a supercomplex of menaquinol-cytochrome c oxidoreductase or the bc 1 complex and aa 3 type cytochrome oxidase [1,[65][66][67]. The bc 1 -aa 3 branch pumps protons out of the cell during transfer of electrons to oxygen, whereas the bd-type oxidase does not pump protons, but has a higher affinity for oxygen [68]. It is assumed that similar to E. coli, the two terminal oxidases of mycobacteria have different affinities for oxygen; however, this awaits experimental validation.

bc 1 -aa 3 Pathway
The bc 1 complex is a key component of the bc 1 -aa 3 pathway, and is required for the bulk of the electron transfer to oxygen during normoxia [69]. bc 1 is encoded by the qcrCAB operon in Mtb. It consists of redox groups comprising a 2Fe/2S centre, located on a Rieske protein (QcrA), two b-type haems (low and high potential) located on a single polypeptide (QcrB), and the haem of cytochrome c 1 (QcrC). QcrA of Mtb has three transmembrane helices and characteristic sequence motifs (CSHLGC and CPCH) of 2Fe-2S Rieske iron-sulfur proteins, QcrB has a 120 amino acid extension at the C-terminus, and the QcrC subunit consists of two haem binding motifs (CVSCH and CASCH) for c-type cytochromes, suggesting a covalent di-haem (bcc) configuration [70]. According to Q-cycle model, oxidation of quinol molecules occurs at the interface of cytochrome b and the 2Fe-2S cluster carrying domain of the Rieske protein, which forms the catalytic center P at the positively charged membrane side of the enzyme [71]. Each quinol molecule oxidizes at the center P, liberating two electrons that move towards different acceptors. One electron proceeds to the iron-sulfur Rieske protein, and is then transferred to cytochrome c, while the other moves to another quinone-binding site on the opposite side of the membrane through haem molecules of variable redox potentials. Complex III releases four protons for every two electrons transferred from menaquinol to cytochrome c into the periplasmic side of the membrane [69].
The Mtb aa 3 -type cytochrome c oxidase is comprised of four subunits: CtaB (a cytochrome c oxidase assembly factor), CtaD (cytochrome c oxidase subunit I containing haem a, a 3 , and copper B (Cu B ), CtaC (cytochrome c oxidase subunit II containing copper A (Cu A ) and CtaE (subunit III). The aa 3 cytochrome c oxidase of Mtb is encoded by ctaBCDE, which is dispersed over different locations of the Mtb genome [72]. Interestingly, the ctaE gene is located immediately upstream of the qcrCAB operon. The putative fourth subunit is encoded by ctaF (Rv2199c). This subunit is co-purified with other subunits in Corynebacterium. Importantly, the deletion mutants display the phenotype similar to CtaC mutant [73]. Four protons are taken up, while two protons are released into the periplasm for every two electrons passing through complex IV [72]. There are three ctaD alleles in Mycobacterium smegmatis, while only one in Mtb, indicating the presence of multiple isoforms of cytochrome c oxidase in M. smegmatis [65]. The proton-pumping type (and energetically more efficient) bc 1 -aa 3 branch of ETC is essential for mycobacteria, as its deletion by homologous recombination is lethal [66]. This observation indicates that the bc 1 -aa 3 branch of ETC is an important drug target.
Unlike E. coli, which utilizes membrane-soluble cytochrome C for transferring electrons between bc 1 complex and aa 3 cytochrome c oxidase, Mycobacterium, Corynebacterium glutamicum, and Rhodococcus do not possess cytochrome C [70,74,75]. Importantly, in C. glutamicum, the QcrC contains an extra binding site for another haem c that may function as a merged cytochrome C [76]. Moreover, subunit II of the oxidase contains an additional 30 amino acids that could participate in the direct interaction between complexes bc 1 and aa 3 , to create a respiratory "supercomplex" [66], obviating the requirement for free cytochrome c [74,76]. Indeed, in C. glutamicum, bc 1 and aa 3 are isolated as a complex that is resistant to detergent treatment [73]. A similar scenario was predicted for mycobacteria [74], and was validated following the isolation of a detergent-resistant supercomplex in M. smegmatis [77]. The interaction between the bc 1 and aa 3 complexes is guided by hydrophobic interactions, while ionic interactions facilitate the alignment between the two complexes for efficient electron transfer from menaquinol to oxygen [77]. Recently, overexpression of the Mtb complex III in M. smegmatis was shown to yield a functional, stable, hybrid supercomplex in the presence of dodecyl maltoside detergent [78]. These observations strongly suggest that, in mycobacterial cells, the bc 1 complex and aa 3 cytochrome c oxidase interact with each other to facilitate the flow of electrons from MK to oxygen, without the requirement of cytochrome C.

Cytochrome bd-Oxidase
Cytochrome bd-type menaquinol oxidase (bd-oxidase) in Mtb is non-proton pumping, and is therefore a less energetically efficient terminal oxidase. E. coli bd-oxidase is the prototypical member of this enzyme class and is encoded by two separate operons, cydAB and cydDC. This is contrary to Mtb, in which all the genes are transcribed from a single cydABDC operon. cydAB encodes the functional cytochromes, while the products of cydDC contribute to cytochrome bd assembly [79]. Furthermore, in E. coli mutants of cydDC, the periplasmic space is more oxidized than in the wild type bacteria [79]. The function of cydDC in mycobacteria is largely unknown, but it plays an important role in mycobacterial persistence in vivo. cydDC mutation reduces the ability of Mtb to survive the transition from acute to chronic infection in mice [12]. Another report suggests that cydDC supports mycobacterial persistence in INH-treated mice [13]. Compared to their wild type counterparts, cytochrome bd mutants of E. coli are sensitive to stress induced by temperature alterations, nitric oxide, H 2 O 2 , and iron (III) chelators. Additionally, they are unable to resume growth upon entering into the stationary phase [80][81][82][83]. Importantly, the growth of cydA mutant M. smegmatis is normal at ambient oxygen levels, but is severely impaired during hypoxia (0.5-1% air saturation). These mutants are also sensitive to cyanide, and are outcompeted when co-cultured with wild type M. smegmatis in its presence [65]. In E. coli, bd-oxidase has a higher affinity for oxygen, and is induced in response to low oxygen tension [84]. Although the affinity of the Mtb bd-oxidase for oxygen has not been determined, its expression is also induced during hypoxia [65]. The expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H 2 O 2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc 1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc 1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91]. Table 1. Summary of potential drug molecules with their respective targets, bactericidal properties, and structures.

Drug Target Properties Structure
Thioridazine NDH-2 MIC 90 -8-15 µg/mL Approved expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H2O2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91]. expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H2O2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91]. expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H2O2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91]. expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H2O2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91]. expression of cydAB is regulated by a SenX3-RegX3 two-component system [85], which also acts as an oxygen-controlled replication switch in Mtb [86]. It was recently demonstrated that Mtb cydAB mutants are sensitive to H2O2 and antibiotic stress [87]. Importantly, drugs inhibiting mycobacterial respiration enhance the expression of cydAB [88]. Interestingly in M. smegmatis, inactivation of bc1 complex results in the upregulation of the bd-type terminal oxidase; however, the bc1 complex does not compensate for the loss of the bd-type oxidase [87]. Genetic inactivation of cytochrome bd-oxidase in various pathogenic microorganisms like Shigella flexneri, Brucella abortus, and Salmonella enterica serovar Typhimurium, impairs their intracellular survival and virulence [89][90][91].

Supercomplex Inhibitors
The essentiality of Mtb cytochrome bc1, along with the large differences in structure and function from its mammalian counterpart, makes it a good target for therapeutic intervention [78]. A variety of antibiotics and chemical compounds have long been known to inhibit the bc1-aa3 complex. The aurachin class of compounds contains quinone analogues, which are reported to inhibit a variety of cytochrome oxidases [56,92,93]. Aurachin D is well-known among this class of compounds

Supercomplex Inhibitors
The essentiality of Mtb cytochrome bc1, along with the large differences in structure and function from its mammalian counterpart, makes it a good target for therapeutic intervention [78]. A variety of antibiotics and chemical compounds have long been known to inhibit the bc1-aa3 complex. The aurachin class of compounds contains quinone analogues, which are reported to inhibit a variety of cytochrome oxidases [56,92,93]. Aurachin D is well-known among this class of compounds

Supercomplex Inhibitors
The essentiality of Mtb cytochrome bc 1 , along with the large differences in structure and function from its mammalian counterpart, makes it a good target for therapeutic intervention [78]. A variety of antibiotics and chemical compounds have long been known to inhibit the bc 1 -aa 3 complex. The aurachin class of compounds contains quinone analogues, which are reported to inhibit a variety of cytochrome oxidases [56,92,93]. Aurachin D is well-known among this class of compounds that inhibit E. coli cytochrome bd-oxidase [92]. It inhibits oxygen consumption in M. smegmatis in a dose-dependent manner [87]. These observations demonstrate the importance of cytochrome bd inhibitors as drug molecules. Optimized derivatives of aurachin D, with better ability to penetrate the mycobacterial cell wall, have a great potential as a new class of antitubercular drugs [87].
Similar to aurachin D, myxothiazol-based compounds, which are isolated from Myxococcus fulvus, target mitochondrial cytochrome b. Myxothiazol interacts both with cytochrome b and an iron-sulfur protein of the complex; this displaces quinone from the high-affinity binding site of the iron-sulfur protein [94]. Importantly, myxothiazol inhibits the growth of Mycobacterium sp. GBF 3 at an MIC value of 6.3 µg/mL [95], indicating that respiratory inhibitors can successfully target mycobacterial species. Interestingly, myxothiazol does not kill Gram-negative or -positive bacteria, but does exhibit strong antifungal activity [95]. A few other antifungal antibiotics, such as mucidin (from Basidiomycetes Oudemansiella mucida) and strobilurin A (from Strobilurin stenacellus) also inhibit the bc 1 complex by binding to the same site as myxothiazol [96]. However, antimycin A (another antibiotic) binds to a different location, inhibiting the oxidation of cytochrome b subunit [97]. A screen in search of new antibiotics identified a novel compound, stigmatellin, from Stigmatella aurantiaca strain Sg a15, that showed activity against various Gram-positive bacteria, yeasts, and filamentous fungi [98]. Subsequently, it was found to block mitochondrial electron transport by inhibiting the cytochrome bc 1 complex as effectively as antimycin A and myxothiazol [99].
The capability of a number of small molecules to specifically target energy production in Mtb has validated oxidative phosphorylation as a viable drug target. Since energy production through oxidative phosphorylation plays an important role in Mtb survival during hypoxia-induced non-replicating persistence [9,100], blockade of oxidative phosphorylation could be pursued, therapeutically, to kill persisters. A number of small molecule inhibitors that specifically target the bc 1 -aa 3 complex have been identified. Several lines of evidence suggest that these inhibitors, which are structural analogues of quinone, target the quinone binding catalytic domains of the bc 1 complex [101]; prominent examples are the imidazo[1,2-a]pyridines (IPs). Using HTS, IPs were identified as potent inhibitors of Mtb and BCG [101]. Importantly, four IP inhibitors were shown to specifically target Mtb strains and BCG, but were not able to kill a number of other Gram-positive and Gram-negative bacteria, or human cell lines, including HepG2 and Neuro2A. Whole genome sequencing of the spontaneous resistant mutants established that these IPs targeted QcrB. These findings were further supported by induction of drug resistance upon overexpression of qcrB [101]. HTS of 100,997 compounds led to the discovery of phenoxyalkylbenzimidazole (PAB) class of compounds showing activity against Mtb with MICs in the nanomolar range, and low cytotoxicity against eukaryotic cells [102,103]. A recent study identified the probable target of PABS to be the QcrB subunit of cytochrome bc 1 oxidase, further confirming it to be an important drug target [104].
An independent screen of more than 100,000 compounds to identify inhibitors of macrophage-resident bacterial growth led to the isolation of two highly active IP compounds with less toxicity than previous molecules of the same class. Further lead optimization resulted in the development of IP Q203, which is specifically active against Mtb (MIC 50 of 2.7 nM in broth cultures and 0.28 nM for intracellular bacteria) [105]. Furthermore, whole genome sequencing of spontaneously resistant mutants revealed the target of Q203 to be the cytochrome bc 1 complex (qcrB) (as depicted in Figure 2). Mutation of Thr313 to either alanine or isoleucine was specifically involved in Q203 resistance [34]. Importantly, this compound was highly active against MDR and XDR clinical isolates [106]. This leading drug candidate has recently progressed to Phase I clinical trials (trial identifier: NCT02858973) under a U.S. FDA Investigational New Drug application. Notably, Q203 is synthesized through a one-pot reaction of 2-aminopyridines or 2-(or 4-)aminopyrimidines, respectively, with 1,2-bis(benzotriazolyl)-1,2-(dialkylamino)-ethanes [107]. These studies were followed by derivatization of imidazo[1,2-a]pyridine 3 carboxyamides into imidazo[1,2-b]thiazole-5-carboxyamides. Structure-activity relationship (SAR)-based assays were utilized for optimizing the imidazo[1,2-b]thiazole-5-carboxyamides to yield three lead compounds that have potent antitubercular activity at low nanomolar concentrations. These compounds specifically inhibit QcrB, and thus, block the growth of replicating Mtb and intracellular mycobacteria, and display very low toxicity [108]. These compounds were also active against large numbers of NTMs, including Mycobacterium avium in murine lungs [108,109]. In another parallel drug discovery effort, an HTS campaign by Novartis Institute for Tropical Diseases identified several potential hit molecules with potent activity against Mtb [110]. However, the presence of ester linkages raised concerns over their metabolic instability [111]. Several compounds were thus optimized for metabolic stability through pharmacokinetic studies in the mouse. These studies led to the development of pyrrolo[3,4-c]pyridine-1,3(2H)-diones having an MIC 90 in the micromolar range [111]. These compounds are hyperactive against cytochrome bd-oxidase mutants, suggesting that they target the bc 1 -aa 3 complex of the respiratory chain. The target for these molecules was identified as qcrB, and a point mutation (Ala317Thr) in qcrB results in resistance to these compounds [111]. Importantly, the target site is identical to that bound by Q203, suggesting that this site may be promiscuous for molecules targeting the bc 1 complex.
Interestingly, in an effort to build a strong pipeline for antimycobacterial drugs, a pool of 1280 FDA approved drugs was screened for growth inhibition of Mtb residing in MRC-5 lung fibroblasts. This screen resulted revealed that the gastric proton pump inhibitor lansoprazole (LPZ, Prevacid) exerted potent activity against Mtb. Importantly, other proton pump inhibitors, such as omeprazole and pantoprazole, were not able to inhibit Mtb growth. It was further demonstrated that LPZ is rapidly converted to the potent antimycobacterial agent, lansoprazole sulfide (LPZS). Thus, LPZ acts as a prodrug that is converted into LPZS within the host cell. As the conversion of LPZ into LPZS is inefficient, a 22-fold higher drug dose was required to kill Mtb cells in broth cultures. LPZS selectively kills Mtb; it is not toxic to closely related NTMs or Gram-positive and-negative bacteria [112]. Target identification studies revealed that LPZS targets the cytochrome bc 1 complex (as depicted in Figure 2) of the respiratory chain, and conversion of leucine-176 to proline in qcrB confers drug resistance. Furthermore, superimposition of the mutant mycobacterial protein structure onto the published QcrB protein from R. sphaeroides [113] revealed that both L176P (a mutation leading to LPZS resistance) and T313A (which confers IPA resistance) were localized to the same site, i.e., the P site, at which ubiquinol oxidation occurs. However, L176P mutants remained susceptible to various IPA, whereas T313A mutants were susceptible to LPZS, revealing different binding mechanisms for each compound [112]. There are conflicting reports on the oral bioavailability of this drug. For example, oral administration of the drug is reported to significantly reduce mycobacterial burden [112], whereas others have shown that intraperitoneal injection of LPZS molecules, rather than the LPZ prodrug, is required to acquire plasma and lung tissue concentrations of the active molecule sufficient to kill Mtb [114]. Recently, the use of LPZ has been associated with reduced incidence of TB when compared with the use of omeprazole or pantoprazole [115]. The ATP synthase inhibitor BDQ and Q203 both result in an increase in mycobacterial oxygen consumption rate (OCR) over a range of physiologically relevant oxygen concentrations [35]. It was suggested that bacteria are compelled to utilize cytochrome bd-oxidase as the terminal oxidase upon disruption of the flow of electrons through the bc1-aa3 pathway by Q203. This postulate is supported by the observation that Q203 completely inhibits respiration in the cytochrome bd-oxidase knockout mutant (cydKO), while the Q203 resistant SNP remains unaffected [35]. Furthermore, cydKO is hypersusceptible to Q203 [116,117]. BDQ and Q203 are relatively slow acting drugs with regard to mycobacterial cytotoxicity. Importantly, BDQ both alone or in combination with Q203, show similar slow kill kinetics, but the combination of CFZ with either BDQ or Q203 results in increased OCR and synergistic killing. The triple combination of CFZ, BDQ, and Q203 kills even faster, and results in complete sterility within 5 days. This combination of drugs is far more effective and rapid than the combination of first-line drugs INH and RIF in culture broths and in infected macrophages. BDQ and Q203 push the cell into reductive stress, during which NADH levels increase, and this potentiates the ROS-generating activity of CFZ, leading to mycobacterial killing [35]. These observations suggest that a combination of drugs targeting different components of the respiratory chain may help in the development of more effective therapeutic regimens. Targeting the bacterium at multiple steps of oxidative phosphorylation may be a better strategy for tackling drug-susceptible as well as MDR and XDR-TB. The role of efflux pumps in Mtb drug resistance is well established [118], and therefore, targeting them may help to resensitize Mtb to antibacterial agents. In support of this, verapamil (an efflux pump inhibitor) increases the potency of Q203 [119].

ATP Synthase
During the transfer of electrons from NADH or succinate to a terminal electron acceptor (such as oxygen), protons are pumped out to generate PMF. ATP synthase can utilize PMF to generate The ATP synthase inhibitor BDQ and Q203 both result in an increase in mycobacterial oxygen consumption rate (OCR) over a range of physiologically relevant oxygen concentrations [35]. It was suggested that bacteria are compelled to utilize cytochrome bd-oxidase as the terminal oxidase upon disruption of the flow of electrons through the bc 1 -aa 3 pathway by Q203. This postulate is supported by the observation that Q203 completely inhibits respiration in the cytochrome bd-oxidase knockout mutant (cydKO), while the Q203 resistant SNP remains unaffected [35]. Furthermore, cydKO is hypersusceptible to Q203 [116,117]. BDQ and Q203 are relatively slow acting drugs with regard to mycobacterial cytotoxicity. Importantly, BDQ both alone or in combination with Q203, show similar slow kill kinetics, but the combination of CFZ with either BDQ or Q203 results in increased OCR and synergistic killing. The triple combination of CFZ, BDQ, and Q203 kills even faster, and results in complete sterility within 5 days. This combination of drugs is far more effective and rapid than the combination of first-line drugs INH and RIF in culture broths and in infected macrophages. BDQ and Q203 push the cell into reductive stress, during which NADH levels increase, and this potentiates the ROS-generating activity of CFZ, leading to mycobacterial killing [35]. These observations suggest that a combination of drugs targeting different components of the respiratory chain may help in the development of more effective therapeutic regimens. Targeting the bacterium at multiple steps of oxidative phosphorylation may be a better strategy for tackling drug-susceptible as well as MDR and XDR-TB. The role of efflux pumps in Mtb drug resistance is well established [118], and therefore, targeting them may help to resensitize Mtb to antibacterial agents. In support of this, verapamil (an efflux pump inhibitor) increases the potency of Q203 [119].

ATP Synthase
During the transfer of electrons from NADH or succinate to a terminal electron acceptor (such as oxygen), protons are pumped out to generate PMF. ATP synthase can utilize PMF to generate ATP from ADP and inorganic phosphate [120,121]. ATP synthase is highly conserved from bacteria to mammals; it consists of a transmembrane F o complex that is used for ion translocation, and a peripheral F 1 complex that catalyzes ATP generation. The membrane-embedded F o complex is made up of a, b, and c subunits that are present in a variable stoichiometry of ab 2 c 8-15 . On the other hand, the hydrophilic F 1 complex consists of 5 subunits in the stoichiometry α 3 β 3 γδ [122]. The proton movement is utilized by the F o subunit to generate rotation of the oligomeric c ring, and this rotation is coupled with the rotation of the attached γ and subunits of the F 1 complex [123][124][125].
During its infection cycle, mycobacteria must survive host-generated stress [126,127]. In order to do so, Mtb utilizes its metabolic flexibility to maintain ATP homeostasis [1,128]. Regulated synthesis of ATP through ATP synthase is critical for the survival of pathogens inside the host. This enzyme is even more important in mycobacterial cells, since they require it for growth on fermentable carbon sources [129]. It must be noted that Mtb downregulates its metabolic rate upon entering macrophages or lung tissue [1,130], and primarily depends on lipids for energy [131]. ATP levels are also reduced in the pathogen upon oxygen and nutrient deficit [9,132]. In the case of Streptococcus, ATP synthase is downregulated during biofilm formation [133], which is believed to harbor dormant bacteria. However, the level of ATP in biofilm-resident Mtb is not significantly different to that observed in planktonic bacteria [134]. Also, single cell microscopy has revealed that antibiotic exposure tremendously reduces the ATP levels in M. smegmatis cells [135]. ATP synthase in mycobacteria is encoded by the atpBEFHAGDC operon (Rv1303-1312). The expression of this operon is tightly regulated to match the cellular ATP requirements, and is therefore closely linked to the respiratory chain activity. This operon is downregulated in hypoxic cultures and in murine lung tissue [8]. Recently, a transcriptional regulator (blaI, Rv1846c) of the atp operon has been identified [136].
The subunit composition of mycobacterial ATP synthase and the mechanism of ATP generation is similar to that of other bacteria, such as E. coli and humans ( Figure 3A). However, there are subtle differences in Mtb ATP synthase that contribute to its survival in humans [137]. These differences, however, can be exploited for the development of Mtb-specific drugs. These differences include the capability to catalyze the ATP biosynthesis at low PMF of~100 mV [9] compared with~200 mV of E. coli [138], and the ability to block ATPase activity [139,140] in order to maintain an optimal level of PMF, as seen in many other bacteria [141]. Mycobacterial ATP synthase has several structural differences compared with the human orthologue. This includes a 36 amino acid C-terminal domain extension of subunit α [142], which is not seen in any other prokaryotes or eukaryotes. Interestingly, this extension is critical for the ability of mycobacterial ATP synthase to reduce ATPase activity. The Mtb ATP synthase γ subunit possesses a unique loop (γ [165][166][167][168][169][170][171][172][173][174][175][176][177][178] ) of 13 amino acids [143] that also inhibits ATP hydrolysis-driven proton pumping [144]. Interestingly, due to the capability of ATP generation at low PMF, Mtb ATP synthase was expected to have a large c ring with more than 10 monomers, as seen in some alkaliphilic bacteria [145][146][147]. However, the crystal structure of the mycobacterial c ring revealed that it contains 9 monomers [148]. This is the smallest bacterial c ring reported with a H + /ATP ratio of 3.
Given the essentiality of ATP synthase for the survival of mycobacteria on fermentable and non-fermentable carbon sources, and its subtle but peculiar structural differences from the mitochondrial ATP synthase present in humans, this enzyme can be utilized as a potential drug target. However, drugs like oligomycin and N,N -dicyclohexylcarbodiimide (DCCD) (that target ATP synthase) are non-selective, and also inhibit generation of ATP in mitochondria and bacteria [149,150], suggesting the conserved nature of this enzyme. Nonetheless, the recent discovery of BDQ as a specific inhibitor of mycobacterial ATP synthase has validated this enzyme complex as a drug target.

Bedaquiline
Several naturally occurring and synthetic ATP synthase inhibitors (such as DCCD) have been described [151]. However, it was only in 2004 [152,153], that an ATP synthase-targeting lead compound of the diarylquinoline class (R207910) was shown to have potent antimycobacterial activity. Four decades after the development of rifabutin in 1975, a new class of antibiotic that specifically targeted tuberculosis was developed by the Andries group [152]. This compound was later called BDQ, or TMC207, and is sold under the brand name of Sirturo ® . specifically targeted tuberculosis was developed by the Andries group [152]. This compound was later called BDQ, or TMC207, and is sold under the brand name of Sirturo ® . Importantly, this compound specifically inhibited the growth of mycobacterial species, and was non-inhibitory to Gram-positive bacteria, such as Nocardia, and Gram-negative bacteria such as E. coli. More importantly, this compound was able to kill MDR and XDR Mtb strains with MICs in the range of 0.03-0.12 µg/mL. Interestingly, usage of 10× MIC for 12 days resulted in a 3-log reduction of bacterial colony forming units (cfu). This killing was time dependent, rather than being concentration dependent. The delayed onset of killing could be explained by the metabolic remodeling of mycobacterial cells upon BDQ exposure. This includes upregulation of the dormancy regulon, ATP synthase, isocitrate lyase, and cytochrome bd oxidase. Additionally, there was a downregulation of DNA/protein biosynthesis and efflux pump expression, in order to conserve energy. The drug also demonstrated significant in vivo activity in a murine model of TB infection. Importantly, its use alone led to a similar reduction of mycobacterial load in the lungs as that of a combination of frontline drugs (RIF + INH + pyrazinamide (PZA)). Moreover, its use in combination with these drugs resulted in a 2-log greater reduction in mycobacterial load compared to the "no BDQ" group. Importantly, BDQ also showed desirable pharmacokinetic properties [152]. Furthermore, use of the Wayne model demonstrated that BDQ efficiently targets both replicating and non-replicating mycobacteria [9]. The drug is posited to be more effective against non-replicating bacteria than those that are actively replicating; this could be due to 5-10-fold lower ATP levels in the non-replicating dormant bacteria [154]. The ability to kill non-replicating bacteria correlates with BDQ's exceptionally high bactericidal activity in mouse TB models [155][156][157], and also with the reduced time for sputum culture conversion in TB patients [158,159].
In an effort to identify the target of BDQ, whole genome sequencing was performed. Mutations in atpE, which codes for the Fo subunit of ATP synthase, were associated with drug resistance [152]. In another target identification effort, mycobacterial membrane extract was passed through a column of BDQ-coated Sepharose beads. This approach also identified subunits of ATP synthase. These experiments were followed by surface plasmon resonance assays, which demonstrated that Importantly, this compound specifically inhibited the growth of mycobacterial species, and was non-inhibitory to Gram-positive bacteria, such as Nocardia, and Gram-negative bacteria such as E. coli. More importantly, this compound was able to kill MDR and XDR Mtb strains with MICs in the range of 0.03-0.12 µg/mL. Interestingly, usage of 10× MIC for 12 days resulted in a 3-log reduction of bacterial colony forming units (cfu). This killing was time dependent, rather than being concentration dependent. The delayed onset of killing could be explained by the metabolic remodeling of mycobacterial cells upon BDQ exposure. This includes upregulation of the dormancy regulon, ATP synthase, isocitrate lyase, and cytochrome bd oxidase. Additionally, there was a downregulation of DNA/protein biosynthesis and efflux pump expression, in order to conserve energy. The drug also demonstrated significant in vivo activity in a murine model of TB infection. Importantly, its use alone led to a similar reduction of mycobacterial load in the lungs as that of a combination of frontline drugs (RIF + INH + pyrazinamide (PZA)). Moreover, its use in combination with these drugs resulted in a 2-log greater reduction in mycobacterial load compared to the "no BDQ" group. Importantly, BDQ also showed desirable pharmacokinetic properties [152]. Furthermore, use of the Wayne model demonstrated that BDQ efficiently targets both replicating and non-replicating mycobacteria [9]. The drug is posited to be more effective against non-replicating bacteria than those that are actively replicating; this could be due to 5-10-fold lower ATP levels in the non-replicating dormant bacteria [154]. The ability to kill non-replicating bacteria correlates with BDQ's exceptionally high bactericidal activity in mouse TB models [155][156][157], and also with the reduced time for sputum culture conversion in TB patients [158,159].
In an effort to identify the target of BDQ, whole genome sequencing was performed. Mutations in atpE, which codes for the F o subunit of ATP synthase, were associated with drug resistance [152]. In another target identification effort, mycobacterial membrane extract was passed through a column of BDQ-coated Sepharose beads. This approach also identified subunits of ATP synthase. These experiments were followed by surface plasmon resonance assays, which demonstrated that atpE binds to a BDQ analogue with a K d of 500 nM [160]. The MIC 50 in the case of human mitochondrial ATP synthase was more than 20,000-fold higher than that of the bacterial enzyme, underscoring the high degree of selectivity afforded by BDQ [161]. A high-resolution crystal structure of mycobacterial ATP synthase, with and without BDQ, has provided insights into the mechanism of BDQ binding, and will also help in structure-based drug design [148]. It was suggested that one or more BDQ molecules could specifically associate with ion binding site of ATP synthase in the c ring of the F o rotor. BDQ interacts with~135 Å 2 of the c ring of the F o rotor consisting of nine residues (Gly62, Leu63, Glu65, Ala66, Ala67, Tyr68, Phe69, Ile70, and Leu72) through van der Waals interactions ( Figure 3B). BDQ's dimethylamino moiety extends into the ion-binding site of C-rings. The interaction is then stabilized by an intermolecular H-bond between the dimethylamino group and Glu65 [148]. BDQ has also been tested against NTM, M. abscessus, which affects cystic fibrosis patients [162], and against M. avium and M. intracellulare [163]. Additionally, it was found to be active against M. abscessus in a zebrafish model of infection [164]. Furthermore, BDQ was effective against M. leprae [165], but bacteriostatic in the case of M. avium [166], and inactive in M. abscessus mouse infection [167,168].
Moreover, BDQ, when combined with other TB drugs, helped to reduce the time of treatment [156,157,169]. A synergistic effect of BDQ and PZA was seen in a mouse model of TB [170]. BDQ was also synergistic with the cell wall synthesis inhibitor, SQ109, as it improved the killing rate and lowered the MIC [171]. Another cell wall synthesis targeting compound, BTZ043, was also found to be synergistic with BDQ in vitro [172]. BTZ043 is a benzothiazinone which inhibits DprE1, an enzyme involved in arabinan biosynthesis [173]. The increased efficacy of BDQ, when used in combination with cell wall biosynthesis inhibitors, could be due to its increased penetration. Furthermore, clinical trials are ongoing for the combination therapy of BDQ with another recently approved drug, delamanid [174]. BDQ results in prolonged QT interval, however, co-administration of RIFor rifapentine with BDQ increases its clearance, likely due to their activity as CYP3A4 inducers, which is involved in BDQ metabolism [175]. Thus, we infer that the currently approved BDQ-RIF combination therapy should be re-evaluated. Furthermore, the addition of ketoconazole, a CYP3A4 inhibitor with BDQ increased the QT interval [176]. A study conducted in human hepatocytes to understand the BDQ metabolism identified N-dealkylation, a novel metabolic pathway. In this study, CYP3A4, CYP2C8, and CYP2C19 were shown to be involved in BDQ N-demethylation [177]. This kind of study is important to understand and prevent BDQ associated adverse drug reactions.
The rate of generation of BDQ-resistant mutants is one in 10 8 bacteria. Certain mycobacteria, including M. novocastrense, M. xenopi, and M. shimoidei, are naturally resistant to BDQ, due to polymorphisms at the atpE locus [168,178]. BDQ resistance is also acquired, and is present in bacteria with cross-resistance to CFZ. This cross-resistance is due to mutations in Rv0678, a transcriptional repressor of the MmpL5 efflux pump [179][180][181]. BDQ is very effective against drug-susceptible MDR and XDR-TB; however, due to its toxicity (attributed to the increased QT interval) and the development of BDQ-resistant strains [182], its use is currently limited to MDR and XDR-TB patients.

Squaramides
After the discovery of BDQ, several studies have been conducted in order to identify novel molecules that target the mycobacterial oxidative phosphorylation pathway [100,101,103,105]. In one example, AstraZeneca employed a luminescence-based method in which inverted membrane vesicles from mycobacteria were charged with NADH and formation of ATP was monitored. Following HTS of 900,000 compounds, two lead classes, namely squaramide and imidazo [1,2-a] pyridine ethers (IPE), were identified. Given their poor solubility, pharmacokinetics, and bacteriostatic nature, IPE were deprioritized, and squaramides were further developed [183]. This resulted in the identification of 31f, which exhibited potent antituberculosis activity (IC 50 of 0.03 µM in proliferation assays). Similar to the case with BDQ, docking studies and the generation of spontaneous mutants identified ATP synthase as the target of squaramide. However, the mode of binding employed by squaramides is different to that of BDQ. 31f is predicted to bind to the interface of ATP synthase subunits a and c. This prediction was supported by the observation that the K179N mutation of subunit a and the D28N mutation of subunit c of ATP synthase engendered resistance to 31f. Importantly, squaramides were also found to be active against single drug resistant clinical strains, including BDQ. Furthermore, squaramides show a similar MIC for BDQ-resistant mutant strains and do not show cross-resistance, suggesting that they interact with a different binding site. Lead compound 31f was also effective in a mouse model of acute TB infection. Further studies are required to see whether squaramides can potentiate the activity of current TB drugs, and whether they will be effective in a chronic TB infection model.

Uncouplers of Proton Motive Force: Pyrazinamide and SQ109
PMF generated by ETC plays a critical role in bacterial growth and survival. During hypoxic growth, dissipation of PMF by uncouplers, such as valinomycin and nigericin, can cause death in the hypoxic cultures [9]. The importance of PMF as a drug target is further validated by the observation that efflux of a number of drugs from mycobacterial cells is dependent on PMF [184]. However, general uncouplers of PMF are not sufficiently selective to be used as antimycobacterial agents, and the development of specific PMF uncouplers remains an area of interest. Importantly, PZA, which is currently being used as a first-line anti-TB drug along with RIF and INH, can disrupt the PMF in mycobacterial cells. Another lead compound, SQ109, also targets the PMF and oxidative phosphorylation [185].
PZA was discovered in the early 1940s, and is effective in mouse models of TB [186] and in TB patients [187]. Consequently, the drug is used as a frontline agent to treat TB. PZA is a prodrug that requires acidic conditions [188] in order to be hydrolyzed by pyrazinamidase or nicotinamidase to pyrazinoic acid (POA), the active form of the drug [189]. PZA-resistant mutants often have variations in the pyrazinamidase gene, which is encoded by pcnA [190][191][192]. PZA is a multitarget drug that dissipates the PMF, inhibits ATP synthesis [193], inhibits membrane transport [194], and also reduces the activity of other proteins [195]. The first clue that PZA could dissipate PMF was the requirement of acidic pH for its bactericidal activity [196]. PZA requires 4-5 fold higher concentration (850 µg/mL) at pH 6.5 compared to pH 5.5 (200 µg/mL) [194]. The active form of the drug, POA, is a weak acid that accumulates inside the cell in acidic conditions [196]. This weakly acidic property helps POA (and the structurally unrelated benzoic acid) to disrupt the membrane potential of Mtb under acidic conditions [194]. In line with this observation, PZA is more active against non-replicating dormant bacteria, as compared to actively replicating bacteria [197] and old cultures [198]. These findings are further supported by the fact that PMF uncouplers synergize with PZA in order to deplete ATP depletion and enhance mycobacterial killing [194]. However, the exact mechanism by which PZA dissipates the membrane potential remains unknown.
SQ109 is structurally related to the frontline TB drug ethambutol, and was developed by Sequella Incorporation in collaboration with Laboratory of Host Defenses, NIH. It has successfully completed phase 2 clinical trials, and is active against drug-susceptible MDR and XDR-TB [199]. Although in TB patients, SQ109 monotherapy for 14 days did not reduce bacterial burden [200], a recent phase 2b-3 clinical study suggested an 80% increase in the culture conversion rate by addition of SQ109 to the standard regimen [201]. Initially, SQ109 was reported to interfere with the assembly of mycolic acid in the mycobacterial cell wall. This was attributed to its ability to target MmpL3, a membrane transporter involved in uptake of trehalose monomycolate [202,203]. However, SQ109 is also active against other organisms, including Helicobacter pylori [204], Trypanosoma cruzi [205], Neisseria gonorrhoeae, Candida albicans [206], and P. falciparum [185]. Since these organisms lack a functional homolog of MmpL3, it is highly likely that MmpL3 is not the primary target of SQ109. This hypothesis is further supported by the absence of spontaneously resistant mutants, a phenomenon observed with many drugs that inhibit multiple targets. Recently, Li et al., have demonstrated that SQ109 and its analogues inhibit MK biosynthesis, and interfere with respiration and generation of PMF [185,205]. It acts as an uncoupler, collapsing both ∆pH and ∆ψ, leading to a decrease in ATP synthesis [185]. Importantly, SQ109 synergizes with BDQ and CFZ, further suggesting that it affects oxidative phosphorylation [206]. Further studies are required to completely understand the complex mechanism by which SQ109 inhibits mycobacterial cell growth.

Conclusions
Despite the presence of alternate respiratory complexes, oxidative phosphorylation is an excellent target for the development of new antimycobacterial drugs (Figure 4). This statement is validated by the presence of a number of its inhibitors in the current TB drug pipeline. Most notable among these inhibitors are Q203 (an inhibitor of bc 1 -aa 3 complex) that has entered clinical trials, and BDQ (ATP synthase inhibitor) that has been approved for treatment of MDR TB. It is worth noting that inhibitors of other components of oxidative phosphorylation, such TPZ&QPs (NDH-2 inhibitor), Ro 48-8071 & DG70 (inhibitors of MK biosynthesis) and SQ109 (PMF disruptor) are potent blockers of mycobacterial growth, in vitro and in vivo. We believe that co-inhibition of parallel respiratory components could significantly shorten the duration of TB treatment. This belief is supported by the enhanced susceptibility of cydAB deletion mutant to BDQ or accelerated killing of Mtb cells by the combination of CFZ with BDQ or Q203. Such combinations shall be tested, in vivo, to further test this hypothesis. suggesting that it affects oxidative phosphorylation [206]. Further studies are required to completely understand the complex mechanism by which SQ109 inhibits mycobacterial cell growth.

Conclusions
Despite the presence of alternate respiratory complexes, oxidative phosphorylation is an excellent target for the development of new antimycobacterial drugs (Figure 4). This statement is validated by the presence of a number of its inhibitors in the current TB drug pipeline. Most notable among these inhibitors are Q203 (an inhibitor of bc1-aa3 complex) that has entered clinical trials, and BDQ (ATP synthase inhibitor) that has been approved for treatment of MDR TB. It is worth noting that inhibitors of other components of oxidative phosphorylation, such TPZ&QPs (NDH-2 inhibitor), Ro 48-8071 & DG70 (inhibitors of MK biosynthesis) and SQ109 (PMF disruptor) are potent blockers of mycobacterial growth, in vitro and in vivo. We believe that co-inhibition of parallel respiratory components could significantly shorten the duration of TB treatment. This belief is supported by the enhanced susceptibility of cydAB deletion mutant to BDQ or accelerated killing of Mtb cells by the combination of CFZ with BDQ or Q203. Such combinations shall be tested, in vivo, to further test this hypothesis.  Schematic representation of the mycobacterial electron transport chain and its inhibitors. NADH derived via glycolysis and tricarboxylic acid (TCA) cycle feds electrons into the electron transport chain by NADH dehydrogenase. The menaquinone (MK) pool can be reduced by primary dehydrogenases such as NADH dehydrogenases (NDH1 and NDH2) and via succinate dehydrogenase (SDH). Electrons from the menaquinone pool are accepted directly by cytochrome bd-type terminal oxidase or via bc 1 -aa 3 supercomplex. A proton motive force (PMF) is generated during electron transport chain because of pumping of protons across the membrane. This PMF is used by the ATP synthase to generate ATP. Drugs that target the oxidative phosphorylation are shown by red flathead arrows. Abbreviations: CFZ, clofazimine; TPZ, trifluoperazine; QPs, quinolinyl pyrimidines; Q203, imidazopyridine amide; LPZ, lansoprazole; Ro 48-8071, (4-bromophenyl)[2-fluoro-4-[[6-(methyl-2-propenylamino)hexyl]oxy]phenyl]-methanone; DG70, biphenyl amide; BDQ, bedaquiline; SQR, squaramide; SQ109, N-adamantan-2-yl-N-((E)-3,7-dimethyl-octa-2,6-dienyl)-ethane-1,2-diamine; PYZ, pyrazinamide. Red arrows in the TCA cycle depict the glyoxylate shunt.
Acknowledgments: AK is supported through Swarnajayanti Fellowship by Department of Science and Technology and CSIR (OLP-0652 and OLP-0141). IKI, SB, are grateful to the CSIR for funding SRF and JRF respectively. AKA is SRF funded by DBT.

Competing Financial Interests:
The authors declare that they do not have any competing financial interests.

Conflicts of Interest:
The authors declare that they do not have any conflict of interest.  Single nucleotide polymorphism  TB  Tuberculosis  TCA cycle  Tricarboxylic acid cycle  TPZ  Trifluoperazine  TZ  Thioridazine  XDR Extremely drug resistant