The C-Terminal Domain of Staphylococcus aureus Zinc Transport Protein AdcA Binds Plasminogen and Factor H In Vitro

Bacterial acquisition of metals from a host is an essential attribute to facilitate survival and colonization within an infected organism. Staphylococcus aureus, a bacterial pathogen of medical importance, has evolved its strategies to acquire multiple metals, including iron, manganese, and zinc. Other important strategies for the colonization and infection of the host have been reported for staphylococci and include the expression of adhesins on the bacterial surface, as well as the acquisition of host plasminogen and complement regulatory proteins. Here we assess the ability of the zinc transport protein AdcA from Staphylococcus aureus, first characterized elsewhere as a zinc-binding protein of the ABC (ATP-binding cassette) transporters, to bind to host molecules. Like other staphylococcus ion-scavenging proteins, such as MntC, a manganese-binding protein, AdcA interacts with human plasminogen. Once activated, plasmin bound to AdcA cleaves fibrinogen and vitronectin. In addition, AdcA interacts with the human negative complement regulator factor H (FH). Plasminogen and FH have been shown to bind to distinct sites on the AdcA C-terminal portion. In conclusion, our in vitro data pave the way for future studies addressing the relevance of AdcA interactions with host molecules in vivo.


Introduction
Staphylococcus aureus is a relevant bacterial pathogen that causes significant morbidity and mortality, especially in a nosocomial environment, where antibiotic-resistant strains are common. The proliferation and full virulence of this pathogen is directly dependent on its ability to scavenge essential transition metals, which are normally chelated from host factors [1].
Transition metal ions, such as iron, manganese, and zinc, are crucial elements involved in diverse biochemical processes in bacterial cells. Zinc is one of the most abundant transition metals in live beings, since many bacterial enzymes, with a determined tridimensional structure, use zinc as a cofactor [2]. Additionally, some works have demonstrated that about 5% of bacterial proteomes are composed of zinc-interacting proteins [3]. Zinc is also important in a well-established single-species staphylococcal biofilm model, in which the SasG protein promotes cell-cell adhesion in a Zn 2+ -dependent manner [4].
Given the capacity to colonize different organs, pathogenic bacteria must deal with fluctuating transition metal concentrations in various host microenvironments [5]. Accurate The AdcA gene was amplified by PCR from the genomic DNA of the S. aureus ATCC 25923 strain. The primers used for the amplification of the sequence encoding the full-length protein and the N-terminal (nucleotides 64-528), intermediate (nucleotides 474-1005), and C-terminal (nucleotides 974-1551) fragments are presented in Table 2. Amplifications were performed with 100 ng of genomic DNA, 200 µM of each dNTP, 0.4 µM of each primer, 1.5 mM MgCl 2 , 1 × Taq buffer, and 2.5 U of Taq DNA polymerase (Thermo Fisher, Waltham, MA, USA) in a total volume of 50 µL. The mixtures were heated to 95 • C for 3 min, and reactions were cycled 30 × for 30 s at 95 • C, 30 s at 60 • C, and 1 min and 40 s at 72 • C. A final extension step was performed for 10 min at 72 • C. PCR fragments were cloned into a pGEM-T Easy vector (Promega, Madison, WI, USA) and subsequently transformed into competent E. coli DH5α cells. Following digestion with BamHI and PstI for the sequence encoding the full-length protein or BamHI and NcoI for the sequences correspondent to the N-terminal, intermediate, and C-terminal fragments, the inserts were subcloned into the pAE vector [18] for recombinant protein expression with an N-terminal 6XHis-tag. All constructs were analyzed by digestion with restriction enzymes besides DNA sequencing of amplicons, generated through the reaction with vector-specific primers. Expression of the recombinant proteins in mid-log-phase cultures of transformed E. coli BL21 (DE3) was achieved by the induction with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG) at 37 • C. After 3 h of incubation, bacterial cells were collected by centrifugation, and the cell pellets were resuspended in a solution composed of 20 mM sodium phosphate (pH 7.4) and 100 mM NaCl, followed by cellular lysis in a sonicator. The soluble and insoluble fractions were isolated by centrifugation at 26,000× g for 15 min. The His-tagged recombinant proteins were purified, from the supernatants, in Ni 2+ -charged chelating Sepharose columns (GE Healthcare, Buckinghamshire, UK). After adsorption of proteins, columns were sequentially washed with solutions containing 20 mM sodium phosphate (pH 7.4), 500 mM NaCl (buffer A), and increasing imidazole concentrations (5,20,40, and 60 mM). The proteins were eluted from the column with a solution comprising buffer A and 1 M imidazole. The eluates were slowly dialyzed against phosphate-buffered saline (PBS), followed by sample analysis utilizing a 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12% SDS-PAGE). Protein concentrations were estimated using the Bradford method (Pierce, Thermo Fisher Scientific Inc., Rockford, IL, USA), according to the manufacturer's instructions. Control proteins (MntC from Staphylococcus aureus and LigA and LigB from Leptospira interrogans) were obtained as previously described [17,19].

Antiserum against Recombinant AdcA
The protocol for obtention of antiserum against recombinant AdcA was approved by the Committee on Ethics of Instituto Butantan (# 9452010316, meeting of 16 March 2016), which in turn is in accordance with Law 11.794, published on 8 October 2008; Decree 6899, made public on 15 July 2009; and the rules issued by the National Council for the Control of Animal Experimentation (CONCEA). A healthy 60-day-old female New Zealand rabbit was immunized intramuscularly with 100 µg of recombinant protein using 2.5 mg of aluminum hydroxide as adjuvant (total volume of 500 µL). Two subsequent booster injections were given at 15-day intervals. The animal was bled from the marginal ear vein prior to immunizations (preimmune bleeding/serum control) and 2 weeks after the third dose. Total bleeding was performed by cardiac puncture under deep terminal anesthesia with ketamine (50 mg/kg) and xylazine (3 mg/kg). The blood was centrifuged for 5 min at 3000× g, and the serum was stored at −20 • C. Antibody titers were determined by enzyme-linked immunosorbent assay (ELISA). Briefly, 96-well plates (high binding, Costar, Corning Inc., Corning, NY, USA) were coated with 1.0 µg/well of purified AdcA and then washed. After blocking with 5% skimmed milk, rabbit serum samples were serially diluted 2-fold beginning with a 1:50 dilution; plates were incubated for 1 h at 37 • C, and then washed. Horseradish peroxidase-conjugated anti-rabbit IgG antibodies (Sigma-Aldrich, St. Louis, MO, USA) were added to the wells. The plates were incubated for an additional hour, and the wells were washed three times. After that, o-phenylenediamine 0.04% (w/v) in citrate phosphate buffer (pH 5.0) plus 0.01% H 2 O 2 was added to the plates. The reaction proceeded for 15 min, and 50 µL of 8 M H 2 SO 4 was added to yield a chromogenic product. An absorbance at 492 nm was determined in a microplate spectrophotometer (Multiskan EX, Labsystems Uniscience, Waltham, MA, USA). were washed thrice with PBS-0.05% Tween 20 (PBS-T) and then blocked with 200 µL of 1% gelatin for 2 h at 37 • C. To assess the dose-dependent attachment of AdcA to plasminogen, protein concentrations varying from 0 to 1 µM were added per well in 100 µL of PBS. After 1.5 h at 37 • C, six washes with PBS-T were performed, followed by the detection of bound AdcA using 100 µL of a 1:5000 dilution of polyclonal specific antiserum produced in rabbit. Incubation proceeded for 1 h, and following three washes with PBS-T, the wells were allowed to incubate for 1 h with 100 µL of a 1:5000 dilution of peroxidase-conjugated goat anti-rabbit immunoglobulin G (IgG) antibody (Sigma-Aldrich, St. Louis, MO, USA) in PBS. All incubations occurred at 37 • C. After washing the wells thrice with PBS-T, the reactions were developed as mentioned above. BSA was used as negative control.

Effects of Ionic Strength and the Role of Lysines in AdcA-Plasminogen Interactions
The role of lysines and ionic strength in AdcA plasminogen interactions was assessed essentially as mentioned above, with some adapted conditions from the procedure described in [17]. Briefly, ELISA plate wells, coated with plasminogen (10 µg/mL), were incubated with AdcA (10 µg/mL) plus ε-aminocaproic acid (0-10 mM) or AdcA (10 µg/mL) plus NaCl (0-800 mM). Bound AdcA was detected as mentioned in the previous section. All experiments were performed in triplicate and repeated, at least, twice. Statistical analysis was performed by using Student's two-tailed t-test, in which a p-value of less than 0.05 was considered statistically significant.

Plasminogen Activation
Plasminogen activation was assessed as previously described [17]. In brief, microtiter plate wells were coated with 10 µg/mL of AdcA, MntC (positive control), or BSA (negative control) for 16-20 h at 37 • C. After blockage with 3% BSA, plasminogen, at a final concentration of 20 µg/mL, was added. After an incubation of 1 h at 37 • C, the wells were washed thrice with PBS-T, followed by the addition of the human urokinase-type plasminogen activator (uPA, Sigma-Aldrich) (3 U/well) plus the chromogenic substrate D-valyl-leucyllysine-r-nitroanilide dihydrochloride (25 µg/well, Sigma-Aldrich), dissolved in PBS. The plates were incubated at 37 • C for 24 h, when the absorbance at 405 nm was determined.

Degradation of Fibrinogen and Vitronectin by Plasmin Bound to AdcA
Recombinant AdcA (10 µg/mL) and BSA (10 µg/mL) were immobilized onto microtiter plate wells for 16-20 h at 37 • C and incubated with plasminogen (20 µg/mL), as described above. After washing the wells three times with PBS-T, human fibrinogen or vitronectin (500 ng), in combination with plasminogen activator uPA (3 U), was added. Reaction components, previously incubated for up to 4 h at 37 • C, underwent separation by 12% SDS-PAGE and transference to nitrocellulose membranes. Products of fibrinogen or vitronectin degradation were detected by Western blotting with anti-human fibrinogen or anti-human vitronectin (1:5000) antibodies, both produced in rabbit. This step was followed by an incubation with a 1:10,000 dilution of peroxidase-conjugated goat anti-rabbit IgG antibody (Sigma-Aldrich). Positive signals were revealed by detection of enhanced chemiluminescence (West Pico, Pierce).

Far Western Blot
Recombinant proteins-full-length AdcA and its fragments-were subjected to separation in a 12% SDS-PAGE, followed by transference to nitrocellulose membranes. The immobilized proteins were then incubated for 1.5 h with 50 µg of purified plasminogen or factor H, both diluted in PBS. After five washes with PBS-T, the membranes were incubated with rabbit anti-human plasminogen (1:3000) or goat anti-human factor H (1:5000) antibodies, followed by appropriate secondary peroxidase-conjugated antibodies (1:10,000). Positive signals were detected by enhanced chemiluminescence (West Pico, Pierce). Purified plasminogen and BSA were included as positive and negative controls, respectively, in plas-minogen experiments, whereas LigA and LigB (two surface-exposed Leptospira proteins) were included as positive controls for FH binding [20].

Competition Assays
In order to assess whether plasminogen and FH compete for the same binding site(s) on AdcA, the recombinant protein-100 µL at a final concentration of 10 µg/mL-was immobilized on ELISA plate wells, as described above. A blockage with 200 µL of 1% gelatin, for 2 h at 37 • C, was followed by the addition of 1 microgram of FH, mixed with different amounts of plasminogen according to FH/plasminogen molar ratios varying from 1:0.25 to 1:4. The assay was accomplished in duplicate, in a way that the AdcA-FH and AdcA-plasminogen complexes could be simultaneously detected in our system. In order to achieve this premise, proteins were incubated with anti-human plasminogen (1:3000) and anti-human FH antibodies (1:5000), followed by interaction with secondary peroxidaseconjugated antibodies (1:5000), as described above. Three independent experiments were performed, each one in duplicate.

AdcA Sequence Analysis
Multiple sequence alignment analysis of AdcA with its counterparts from Streptococcus pneumoniae, Streptococcus suis, Enterococcus faecalis, and Staphylococcus haemolyticus and with the single-domain protein ZnuA from Salmonella enterica and ZinT from Salmonella enterica, Escherichia coli, and Bacillus subtilis was performed with Clustal Omega (https: //www.ebi.ac.uk/Tools/msa/clustalo/ (accessed on 20 December 2021). The S. aureus AdcA N-terminal domain harbors the conserved residues involved in metal binding, also present in the four above-mentioned double-domain proteins and the single-domain ZnuA: His 67 , His 154 , His 218 , and Glu 292 , besides the His-rich region observed in the zincbinding SBP family (Supplementary Figure S1). The AdcA C-terminal portion harbors three additional histidines (His 468 , His 477 , His 479 ), also related to metal binding and present in other double-domain and ZinT proteins analyzed [13].

Expression and Purification of Full-Length AdcA and Its Fragments
In order to identify functional domains of AdcA, three recombinant fragments were produced. The criterion to split the sequence was based on the size of the fragments, and we aimed to maintain structural and functional specificities. The residues involved in metal binding are present in all three fragments: the N-terminal contains the residues His 67 and His 154 and the conserved His-rich region, the intermediate fragment contains a coiled-coil domain and the residues His 218 and Glu 292 , and the C-terminal portion of AdcA harbors the histidines of the cited ZinT proteins [13]. All fragments have overlapping regions, highlighted in bold ( Figure 1A). Purified full-length AdcA and its recombinant fragments appear as single major bands in SDS-PAGE, and the observed mobilities correspond to their calculated molecular masses: 59 kDa (full-length AdcA), 19 kDa (N-terminal fragment), 22 kDa (intermediate fragment), and 24 kDa (C-terminal fragment) ( Figure 1B).

AdcA Interacts with Plasminogen
In a previous work, we demonstrated that MntC, a S. aureus manganese transport protein, is capable of binding to several extracellular matrix and coagulation cascade components, including laminin, collagen type IV, cellular and plasma fibronectin, plasminogen, and fibrinogen [17]. This prompted us to evaluate whether AdcA, a zinc transport protein, would also display binding properties. The interaction of AdcA with the above-mentioned host components was then investigated, and a dose-dependent and saturable binding to plasminogen was observed (Figure 2A). The apparent K D of this interaction was calculated in GraphPad Prism using the best fit to total binding saturation function. In these adjustment conditions, the same order of magnitude for AdcA (K D = 45.09 nM) and MntC, used as a control (K D = 14.75 nM) was observed. No significant binding activity to other

AdcA Interacts with Plasminogen
In a previous work, we demonstrated that MntC, a S. aureus manganese transpor protein, is capable of binding to several extracellular matrix and coagulation cascade components, including laminin, collagen type IV, cellular and plasma fibronectin, plasminogen, and fibrinogen [17]. This prompted us to evaluate whether AdcA, a zinc transport protein, would also display binding properties. The interaction of AdcA with the above-mentioned host components was then investigated, and a dose-dependent and saturable binding to plasminogen was observed (Figure 2A). The apparent KD of this interaction was calculated in GraphPad Prism using the best fit to total binding saturation function. In these adjustment conditions, the same order of magnitude for AdcA (KD = 45.09 nM) and MntC, used as a control (KD = 14.75 nM) was observed. No significant The kringle domains of plasminogen may interact with lysine residues present on diverse receptors [21]. Given the high content of such residues in AdcA (16%), we assessed whether AdcA-plasminogen interactions would be affected by the presence of lysine analog ε-aminocaproic acid. As depicted in Figure 2B, EACA markedly inhibited plasminogen-AdcA interactions in a dose-dependent manner, suggesting that the lysine-binding sites on the kringle domains of plasminogen may be involved in the event. The role of ionic strength in AdcA-plasminogen interactions was also evaluated. Salt concentrations greater than 400 mM significantly decreased AdcA binding to its target ( Figure 2C). It appears that competitive ionic or ion-dipole interactions with lysine residues of AdcA might occur in higher ionic strength.
sessed whether AdcA-plasminogen interactions would be affected by the presence of lysine analog ε-aminocaproic acid. As depicted in Figure 2B, EACA markedly inhibited plasminogen-AdcA interactions in a dose-dependent manner, suggesting that the lysine-binding sites on the kringle domains of plasminogen may be involved in the event. The role of ionic strength in AdcA-plasminogen interactions was also evaluated. Salt concentrations greater than 400 mM significantly decreased AdcA binding to its target ( Figure 2C). It appears that competitive ionic or ion-dipole interactions with lysine residues of AdcA might occur in higher ionic strength. . AdcA-plasminogen complexes were detected with polyclonal anti-AdcA serum, followed by peroxidase-conjugated secondary antibodies. Data show the mean absorbance value at 492 nm ± the standard deviation of three independent experiments, with correspondent duplicates. For this analysis, a Student's t-test was used, in which * p < 0.05 was considered statistically significant.

AdcA-Bound Plasminogen Is Activated to Plasmin
In vivo, after binding to its receptors, plasminogen is converted to its active form, plasmin, by plasminogen activators (uPA, tPA) [22]. As shown in Figure 3, plasminogen could be activated by uPA once bound to immobilized AdcA. Consequently, the freshly generated plasmin was able to cleave the chromogenic substrate D-valyl-leucyl-lysine-r-nitroanilide dihydrochloride (Sub). S. aureus MntC, previously shown to be a plasminogen-binding protein [17], was included as a positive control, and BSA as a negative one. No detectable cleavage of the chromogenic substrate was disclosed in the presence of plasminogen activator inhibitor 1 (PAI-1) or in the absence of uPA, plasminogen, or both uPA and plasminogen, regardless of the protein used for coating (BSA, AdcA, or MntC) (Figure 3). AdcA (10 µg/mL) was added to plasminogen-coated wells in the presence of ε-aminocaproic acid (0.1-10 mM) or NaCl (25-800 mM). AdcA-plasminogen complexes were detected with polyclonal anti-AdcA serum, followed by peroxidase-conjugated secondary antibodies. Data show the mean absorbance value at 492 nm ± the standard deviation of three independent experiments, with correspondent duplicates. For this analysis, a Student's t-test was used, in which * p < 0.05 was considered statistically significant.

AdcA-Bound Plasminogen Is Activated to Plasmin
In vivo, after binding to its receptors, plasminogen is converted to its active form, plasmin, by plasminogen activators (uPA, tPA) [22]. As shown in Figure 3, plasminogen could be activated by uPA once bound to immobilized AdcA. Consequently, the freshly generated plasmin was able to cleave the chromogenic substrate D-valyl-leucyl-lysine-r-nitroanilide dihydrochloride (Sub). S. aureus MntC, previously shown to be a plasminogen-binding protein [17], was included as a positive control, and BSA as a negative one. No detectable cleavage of the chromogenic substrate was disclosed in the presence of plasminogen activator inhibitor 1 (PAI-1) or in the absence of uPA, plasminogen, or both uPA and plasminogen, regardless of the protein used for coating (BSA, AdcA, or MntC) (Figure 3).

Plasmin Bound to AdcA Cleaves Fibrinogen and Vitronectin
It is well established that active plasmin has a variety of physiological functions, playing a decisive role in fibrinolysis, wound healing, tissue remodeling, inflammation, and ECM degradation (for a recent review, see [23]). In the present study, we assessed the degradation of human fibrinogen and vitronectin by plasmin(ogen) bound to immobilized AdcA. According to our results, AdcA-bound plasmin degraded the fibrinogen β-chain after 2 h of incubation ( Figure 4A) and time-dependently degraded human vitronectin, generating 61-63 and 35-36 kDa cleavage fragments ( Figure 4B). No cleavage products were observed in the absence of the substrates or uPA ( Figure 4A,B). BSA was used as a negative control, and no degradation products were observed in this condition as well ( Figure 4A,B).
It is worth mentioning that uPA comigrates with fibrinogen α-chain, which migrates as a 73 kDa band, but is not visible in Figure 4A, lane 5 (this lane contains plasminogen and fibrinogen, but not uPA). Figure 3. Active plasmin is produced from AdcA-bound plasminogen. Microtiter plate wells, previously sensitized with recombinant proteins or BSA (10 µ g/mL), were incubated with plasminogen (20 µ g/mL). The wells were washed with PBS-T, and plasmin activity was assessed upon addition of the chromogenic substrate D-valyl-leucyl-lysine-r-nitroanilide dihydrochloride (Sub) (25 µ g/well) and the plasminogen activator uPA (3 U)). Control reactions: (PLG + uPA + PAI-1 + Sub)-plasminogen activator inhibitor 1 (PAI-1), which inhibits uPA, was added along with uPA and Sub; (PLG + Sub)-uPA was omitted, preventing the conversion of plasminogen to plasmin; (uPA + Sub)-plasminogen was omitted, preventing substrate cleavage; (Sub)-both uPA and plasminogen were omitted. Data show the mean absorbance value at 405 nm ± the standard deviation of three independent experiments, with correspondent duplicates. For this analysis, a Student's t-test was used, in which * p < 0.05 was considered statistically significant. PAI-1 is the plasminogen activator inhibitor 1.

Plasmin Bound to AdcA Cleaves Fibrinogen and Vitronectin
It is well established that active plasmin has a variety of physiological functions, playing a decisive role in fibrinolysis, wound healing, tissue remodeling, inflammation, and ECM degradation (for a recent review, see [23]). In the present study, we assessed the degradation of human fibrinogen and vitronectin by plasmin(ogen) bound to immobilized AdcA. According to our results, AdcA-bound plasmin degraded the fibrinogen β-chain after 2 h of incubation ( Figure 4A) and time-dependently degraded human vitronectin, generating 61-63 and 35-36 kDa cleavage fragments ( Figure 4B). No cleavage products were observed in the absence of the substrates or uPA (Figure 4A,B). BSA was Figure 3. Active plasmin is produced from AdcA-bound plasminogen. Microtiter plate wells, previously sensitized with recombinant proteins or BSA (10 µg/mL), were incubated with plasminogen (20 µg/mL). The wells were washed with PBS-T, and plasmin activity was assessed upon addition of the chromogenic substrate D-valyl-leucyl-lysine-r-nitroanilide dihydrochloride (Sub) (25 µg/well) and the plasminogen activator uPA (3 U)). Control reactions: (PLG + uPA + PAI-1 + Sub)-plasminogen activator inhibitor 1 (PAI-1), which inhibits uPA, was added along with uPA and Sub; (PLG + Sub)-uPA was omitted, preventing the conversion of plasminogen to plasmin; (uPA + Sub)-plasminogen was omitted, preventing substrate cleavage; (Sub)-both uPA and plasminogen were omitted. Data show the mean absorbance value at 405 nm ± the standard deviation of three independent experiments, with correspondent duplicates. For this analysis, a Student's t-test was used, in which * p < 0.05 was considered statistically significant. PAI-1 is the plasminogen activator inhibitor 1.

AdcA Interacts with Plasminogen and FH through Its C-Terminal Domain
With the aim of mapping the AdcA region(s) responsible for plasminogen-binding activity, a ligand affinity blot was performed with the three AdcA fragments shown in Figure 1. The C-terminal fragment was the only region that interacted with plasminogen, revealing that the binding capacity of AdcA to plasminogen is conferred by this portion (Figure 5A), which contains the three histidines involved in zinc binding, also found in the double-domain streptococcal AdcA proteins. Purified plasminogen was included as a positive control, and BSA was used as a negative one.

AdcA Interacts with Plasminogen and FH through Its C-Terminal Domain
With the aim of mapping the AdcA region(s) responsible for plasminogen-binding activity, a ligand affinity blot was performed with the three AdcA fragments shown in Figure 1. The C-terminal fragment was the only region that interacted with plasminogen, revealing that the binding capacity of AdcA to plasminogen is conferred by this portion (Figure 5A), which contains the three histidines involved in zinc binding, also found in the double-domain streptococcal AdcA proteins. Purified plasminogen was included as a positive control, and BSA was used as a negative one.
Considering that staphylococci employ diverse strategies to avoid innate immune responses, such as complement deposition, and given that several plasminogen-binding proteins not infrequently are additionally able to interact with factor H, we evaluated the interaction of Staphylococcus AdcA with this important complement regulatory protein (factor H). The capacity of full-length AdcA to bind to FH was initially examined. For this assay, Leptospira LigA and LigB were used as positive controls [19]. As seen in Figure 5B, AdcA interacted with FH, and binding was mostly mediated by its C-terminal portion ( Figure 5C).  (3), and AdcA C-terminal fragment (4) were incubated with purified human FH and probed with anti-human FH antibody as described above.

Plasminogen and FH Do Not Share Binding Sites on AdcA
Given that both plasminogen and FH interact with the C-terminal portion of AdcA, we then assessed whether they compete for the same binding sites. For this, a fixed amount of FH (1 µ g) and increasing quantities of plasminogen (0-4 µ g) were added to the immobilized recombinant protein. Plasminogen and FH were independently detected with specific antibodies. As FH interaction with AdcA was not affected by the  (3), BSA (4), and purified plasminogen (5) were subjected to SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was incubated with purified plasminogen (50 µg) and probed with antihuman plasminogen antibody, followed by the addition of corresponding secondary HRP-conjugated antibodies. (B) Full-length AdcA (1), BSA (2), LigA (3), and LigB (4) were subjected to SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was incubated with purified factor H (50 µg) and probed with anti-human FH antibody, followed by the addition of corresponding secondary HRP-conjugated antibodies. (C) Full-length AdcA (1), AdcA N-terminal fragment (2), AdcA intermediate fragment (3), and AdcA C-terminal fragment (4) were incubated with purified human FH and probed with anti-human FH antibody as described above.
Considering that staphylococci employ diverse strategies to avoid innate immune responses, such as complement deposition, and given that several plasminogen-binding proteins not infrequently are additionally able to interact with factor H, we evaluated the interaction of Staphylococcus AdcA with this important complement regulatory protein (factor H). The capacity of full-length AdcA to bind to FH was initially examined. For this assay, Leptospira LigA and LigB were used as positive controls [19]. As seen in Figure 5B, AdcA interacted with FH, and binding was mostly mediated by its C-terminal portion ( Figure 5C).

Plasminogen and FH Do Not Share Binding Sites on AdcA
Given that both plasminogen and FH interact with the C-terminal portion of AdcA, we then assessed whether they compete for the same binding sites. For this, a fixed amount of FH (1 µg) and increasing quantities of plasminogen (0-4 µg) were added to the immobilized recombinant protein. Plasminogen and FH were independently detected with specific antibodies. As FH interaction with AdcA was not affected by the presence of increasing amounts of plasminogen, we conclude that plasminogen and FH do not have overlapping binding sites on AdcA ( Figure 6). N-terminal fragment (1), AdcA intermediate fragment (2), AdcA C-terminal fragm and purified plasminogen (5) were subjected to SDS-PAGE and transferred to membrane. The membrane was incubated with purified plasminogen (50 μg) and p ti-human plasminogen antibody, followed by the addition of correspond HRP-conjugated antibodies. (B) Full-length AdcA (1), BSA (2), LigA (3), and Lig jected to SDS-PAGE and transferred to a nitrocellulose membrane. The membrane with purified factor H (50 μg) and probed with anti-human FH antibody, followed of corresponding secondary HRP-conjugated antibodies. (C) Full-length Ad N-terminal fragment (2), AdcA intermediate fragment (3), and AdcA C-terminal fra incubated with purified human FH and probed with anti-human FH antibody as d

Plasminogen and FH Do Not Share Binding Sites on AdcA
Given that both plasminogen and FH interact with the C-terminal po we then assessed whether they compete for the same binding sites. Fo amount of FH (1 µ g) and increasing quantities of plasminogen (0-4 µ g) w the immobilized recombinant protein. Plasminogen and FH were inde tected with specific antibodies. As FH interaction with AdcA was not a presence of increasing amounts of plasminogen, we conclude that plasmin do not have overlapping binding sites on AdcA ( Figure 6).

Discussion
Understanding the mechanisms of the pathogenicity of S. aureus is essential for the development of more effective therapies in order to prevent and eliminate infections caused by this bacterium. S. aureus survival and virulence in the host depend on the bacterial ability to acquire essential metals, especially iron, manganese, and zinc, normally scarce in the infection sites [1].
Zinc homeostasis is controlled by a delicate balance to maintain stable levels of intracellular transition metal [7]. Although an excess of this metallic ion would compromise the perfect functioning of cell machinery, adequate concentration of zinc is a prerequisite for the full activity of many enzymes and transcription factors, as well as for bacterial metabolism, and for anti-inflammatory processes responsive to immune actions triggered by the host. In this work, we describe a new function for the zinc-binding surface protein of S. aureus AdcA, a member of the metal-binding receptor family (MBR). Unlike other MBRs, the adcA gene is not part of an ABC transporter operon, raising the hypothesis that AdcA may share the integral membrane protein and the cytoplasmic ATPase with another staphylococcal metal-binding receptor. Homologous S. pyogenes AdcA and S. pneumoniae AdcA share 61% of identical residues, and sequence alignment analysis of these proteins with S. aureus AdcA revealed a 37% degree of similarity. Most importantly, S. aureus AdcA conserves the histidine residues in both N-and C-termini, besides the amino acids aspartate or glutamate present in the C-terminal portion, collectively involved in metal binding. This array of amino acids is the fingerprint of zinc-interacting proteins [24], especially metal-binding receptor family members.
In addition to nutrient uptake functions, such as transition metals, proteins present on the surface of S. aureus play crucial roles in colonization, the establishment of infection, and immune evasion by interacting with host molecules of the uppermost cellular layer, extracellular matrix, and complement system. Well-characterized adhesins of S. aureus belong to the MSCRAMM (microbial surface components recognizing adhesive matrix molecules) family [25,26]. These family members harbor a conserved C-terminal cell wall sorting signal containing a Leu-Pro-X-Thr-Gly (LPXTG) motif, which is the target of an enzymatic factor, sortase A, involved in surface protein anchoring to membrane [27]. Examples of MSCRAMMs include clumping factors A (ClfA) and B (ClfB) responsible for fibrinogen binding, fibronectin-binding proteins A (FnBPA) and B (FnBPB) (reviewed in [28]), and collagen adhesin (Cna) [29].
Interaction of bacterial pathogens with the host's plasminogen and its conversion to plasmin allows bacteria to invade the host by cleaving tissue components and fibrin clots and escaping the host's defenses by removing IgG and C3b opsonins [30]. Plasminogen can be captured by the cell surface of S. aureus where the zymogen can be activated by staphylokinase or host urokinase and t-PA [31]. The binding of multifunctional proteins anchored and not anchored to the surface of S. aureus to plasminogen has been described. Enolase, triosephosphate isomerase (TPI), and GAPDH, which are proteins of the glycolytic pathway, can migrate to the surface of S. aureus and interact with plasminogen [32]. Plasmin, the active form of plasminogen with serine protease activity, has in its structure five tripledisulfide-bonded kringle domains containing lysine-binding sites (LBSs) [33]. C-terminal lysyl residues mediate the binding of different receptors to plasminogen, including αenolase of S. pneumoniae and S. pyogenes [34,35].
In this work, we demonstrated that AdcA from S. aureus binds human plasminogen in a dose-dependent and saturable manner. The magnitude of apparent K D values encountered for the protein fell within the nanomolar range, thus indicating that they bind to plasminogen with high affinity. Based on these findings, we can anticipate that AdcA may act as a S. aureus virulence factor by recruiting plasminogen to the staphylococcal surface, which would be engaged in triggering a sequence of events necessary for bacteria spread. Blockage of plasminogen lysine-binding sites by EACA and incubation of the two proteins under high salt concentrations significantly inhibited AdcA-plasminogen interactions, pointing to a role of lysine residues and adequate ionic strength in AdcA binding to plasminogen. Active plasmin was generated from AdcA-bound plasminogen in the presence of uPA, as attested by the proteolytic cleavage of a synthetic chromogenic plasmin substrate. It is well known that plasmin has a variety of physiological functions, playing a crucial role in fibrinolysis, wound healing, tissue remodeling, inflammation, and ECM degradation (for a recent review, see [23]). Pathogenic bacteria take advantage of this host mechanism to gain access to deeper tissues, for instance, lungs, blood, heart, or even bones. Their journey, quite often, initiates in colonizing mucosal sites, such as the nasopharynx, where bacterial species live as commensals until they capture all nutrients the invasion demands. In this stage, the infection episodes are usually asymptomatic. The existence of carriers in these conditions constitutes a great challenge to epidemiological vigilance and transmission control [36].
In this work, further evidence of plasmin activity was provided by the degradation of the physiological substrates-fibrinogen and vitronectin-by plasmin bound to im-mobilized AdcA. Complete degradation of human fibrinogen β-chain occurred, which displayed a profile similar to the one observed for fibrinogen cleavage by MntC-bound plasmin(ogen) [17]. Degradation fragments for human vitronectin were also observed, and the cleavage pattern seems to be compatible with the expected profile of vitronectin plasminolysis. According to Kost et al. (1996) [37], the proteolytic action of plasmin on vitronectin first produces 61-63 kDa fragments upon removal of C-terminal residues of the molecule, followed by a second round of cleavage, which generates 35-56 kDa fractions as a consequence of the N-terminal vitronectin processing.
The AdcA region(s) involved in plasminogen binding were assessed, and according to our data, this interaction is clearly mediated by the AdcA C-terminal region. Defining the precise location of lysines involved in plasmin(ogen) binding is still a perspective for a future work. Our results provide evidence that lysine residues play a relevant role in plasmin(ogen)-AdcA interactions. However, whether additional residues would contribute to this binding remains uncertain. It is suggested that positively charged residues -not only lysines -in a hydrophobic environment may contribute to plasminogen binding in an effective way (reviewed in [21]). Staphylococci are highly efficient bacteria, equipped with multiple strategies to infect and persist in the host. It is worth mentioning that S. aureus, in particular, expresses multifunctional surface-exposed proteins that have been shown to interact with two or more plasma proteins simultaneously, including complement regulatory proteins and plasminogen (reviewed in [38]). In this context, AdcA also interacted with human factor H, an important soluble regulator of the alternative pathway of the complement system. Acquisition of FH by pathogenic bacteria is crucial for survival in the bloodstream during infection, and S. aureus has been shown to express several FH-binding proteins, such as Efb, Sbi, and SdrE [39][40][41].
In conclusion, in this work we provide evidence of a novel function for S. aureus AdcA. Besides contributing to zinc homeostasis, AdcA may help this important human pathogen to invade and colonize the human host by acquiring plasminogen and the negative complement regulator FH. Although our data support the hypothesis that AdcA is a relevant S. aureus virulence factor, it remains to be investigated whether AdcA may serve, like MntC, as a component for a vaccine formulation against Staphylococcus infections.