Design, Synthesis, and Antiprotozoal Evaluation of New Promising 2,9-Bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline Derivatives, a Potential Alternative Scaffold to Drug Efflux

A series of novel 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives was designed, synthesized, and evaluated in vitro against three protozoan parasites (Plasmodium falciparum, Leishmania donovani and Trypanosoma brucei brucei). Pharmacological results showed antiprotozoal activity with IC50 values in the sub and μM range. In addition, the in vitro cytotoxicity of these original molecules was assessed with human HepG2 cells. The substituted diphenylphenanthroline 1l was identified as the most potent antimalarial derivative with a ratio of cytotoxic to antiparasitic activities of 505.7 against the P. falciparum CQ-resistant strain W2. Against the promastigote forms of L. donovani, the phenanthrolines 1h, 1j, 1n and 1o were the most active with IC50 from 2.52 to 4.50 μM. The phenanthroline derivative 1o was also identified as the most potent trypanosomal candidate with a selectivity index (SI) of 91 on T. brucei brucei strain. FRET melting and native mass spectrometry experiments evidenced that the nitrogen heterocyclic derivatives bind the telomeric G-quadruplexes of P. falciparum and Trypanosoma. Moreover, as the telomeres of the parasites P. falciparum and Trypanosoma could be considered to be possible targets of this kind of nitrogen heterocyclic derivatives, their potential ability to stabilize the parasitic telomeric G-quadruplexes have been determined through the FRET melting assay and by native mass spectrometry.


Faculty of P
armacy, Agents Infectieux, Résistance et Chimiothérapie (AGIR),



University of Picardie Jules Verne,



5 Laboratoire d'Optique et Biosciences, Institut Polytechnique de Paris, Ecole Polytechnique, CNRS, INSERM,



6 Université de Pau et des Pays de l'Adour, E2S UPPA, CNRS, IPREM,



7 University of Bordeaux, CNRS, INSERM,



8 Centre de Recherche Cardio-thoracique de Bordeaux (CRCTB), UMR U1045 INSERM,



9 INRAE Bordeaux Aquitaine,


Design, Synthesis, and Antiprotozoal Evaluation of New Promising 2,9-Bis[(substituted-aminomethyl)]-4,7-phenyl-1,10phenanthroline Derivatives, a Potential Alternative Scaffold to Drug Efflux
13 November 20226ECA8B7FA0EB2982D84F8A543FB7F9BD10.3390/pathogens11111339Received: 5 October 2022 Accepted: 7 November 2022antimalarial activityphenanthrolineG-quadruplexantileishmanial activityantitrypanosomal activity
A series of novel 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives was designed, synthesized, and evaluated in vitro against three protozoan parasites (Plasmodium falciparum, Leishmania donovani and Trypanosoma brucei brucei).Pharmacological results showed antiprotozoal activity with IC 50 values in the sub and µM range.In addition, the in vitro cytotoxicity of these original molecules was assessed with human HepG2 cells.The substituted diphenylphenanthroline 1l was identified as the most potent antimalarial derivative with a ratio of cytotoxic to antiparasitic activities of 505.7 against the P. falciparum CQ-resistant strain W2.Against the promastigote forms of L. donovani, the phenanthrolines 1h, 1j, 1n and 1o were the most active with IC 50 from 2.52 to 4.50 µM.The phenanthroline derivative 1o was also identified as the most potent trypanosomal candidate with a selectivity index (SI) of 91 on T. brucei brucei strain.FRET melting and native mass spectrometry experiments evidenced that the nitrogen heterocyclic derivatives bind the telomeric G-quadrup exes of P. falciparum and Trypanosoma.Moreover, as the telomeres of the parasites P. falciparum and Trypanosoma could be considered to be possible targets of this kind of nitrogen heterocyclic derivatives, their potential ability to stabilize the parasitic telomeric G-quadruplexes have been determined through the FRET melting assay and by native mass spectrometry.

Introduction

At present, no single tool is available to solve the public health problem of malaria [1], which caused 241 million cases and 627,000 deaths in 2020 worldwide [2].This mortality indicator is significantly lower than in 2000 (896,000 deaths) even if there were

Introduction
At present, no single tool is available to solve the public health problem of malaria [1], which caused 241 million cases and 627,000 deaths in 2020 worldwide [2].This mortality indicator is significantly lower than in 2000 (896,000 deaths) even if there were an estimated 14 million more malaria cases and 47,000 more deaths in 2020 compared to 2019 due to disruptions to services during the pandemic [2].In response, the World Health Organization (WHO) recently updated its recommendations, particularly in terms of prevention, which includes preventive chemotherapies, vector control and vaccination [3].(i) Indeed, young children still represent a significant proportion of the population at risk as the percentage of total malaria deaths in children aged under 5 years reduced from 87% in 2000 to 77% in 2020 [1].To contribute to their prevention, since October 2021, the WHO recommended that the RTS,S/AS01 malaria vaccine be used for the prevention of P. falciparum malaria in children living in regions with moderate to high transmission [4].If introduced widely and urgently, the RTS,S/AS01 vaccine could save thousands of children's lives every year.(ii) Moreover, concerning the vector control, manufacturers delivered about 229 million insecticide-treated mosquito nets to malaria endemic countries in 2020 [2].Of these, an increasing percentage of 19.4% was represented by pyrethroid-piperonyl butoxide nets (12.4% more than in 2019) and 5.2% were dual active ingredient insecticide-treated mosquito nets (3.6% more than in 2019) [2].Then, the percentage of the population sleeping under an insecticide-treated mosquito net increased considerably between 2000 and 2020, for the whole population (from 2% to 43%) and for the population at risk (from 3% to 49% for children aged under 5 years and from 3% to 49% for pregnant women) [2].(iii) Chemoprevention strategies are also currently recommended and evaluated by the WHO [5].
an estimated 14 million more malaria cases and 47,000 more deaths in 2020 compared to 2019 due to disruptions to services during the pandemic [2].In response, the World Health Organization (WHO) recently updated its recommendations, particularly in terms of prevention, which includes preventive chemotherapies, vector control and vaccination [3].(i) Indeed, young children still represent a significant proportion of the population at risk as the percentage of total malaria deaths in children aged under 5 years reduced from 87% in 2000 to 77% in 2020 [1].To contribute to their prevention, since October 2021, the WHO recommended that the RTS,S/AS01 malaria vaccine be used for the prevention of P. falciparum malaria in children living in regions with moderate to high transmission [4].If introduced widely and urgently, the RTS,S/AS01 vaccine could save thousands of children's lives every year.(ii) Moreover, concerning the vector control, manufacturers delivered about 229 million insecticide-treated mosquito nets to malaria endemic countries in 2020 [2].Of these, an increasing percentage of 19.4% was represented by pyrethroid-piperonyl butoxide nets (12.4% more than in 2019) and 5.2% were dual active ingredient insecticide-treated mosquito nets (3.6% more than in 2019) [2].Then, the percentage of the population sleeping under an insecticide-treated mosquito net increased considerably between 2000 and 2020, for the whole population (from 2% to 43%) and for the population at risk (from 3% to 49% for children aged under 5 years and from 3% to 49% for pregnant women) [2].(iii) Chemoprevention strategies are also currently recommended and evaluated by the WHO [5].

Nevertheless, despite these numerous prevention efforts, the increasing multidrug resistance of Plasmodium parasites worldwide is worrying and contributes strongly to the WHO's concern that the 2030 targets of the WHO's global malaria strategy will not be met [2].Indeed, molecular markers of Plasmodium falciparum resistance to antimalarial drugs have been found to be associated with reduced treatment response and are useful in surveillance for resistance [5].Thus, among the tools to be developed and prioritized to combat malaria, more effective antimalarial medicines with new potential mechanisms of action are urgently needed [6].

In this context, efforts to discover new 4-aminoquinoline derivatives are ongoing.Indeed, it is unlikely that the parasite will be able to evolve resistance to drugs targeting the pathway involved in hemoglobin degradation.Previous studies showed that modulation and modification of the lateral side chain of chloroquine (CQ) or of mefloquine (MQ) that led to original aminoquinoline compounds avoid the CQ resistance mechanism [7][8][9][10].Another original strategy is to design and synthesize quinoline-based drugs that are not recognized by the protein system Nevertheless, despite these numerous prevention efforts, the increasing multidrug resistance of Plasmodium parasites worldwide is worrying and contributes strongly to the WHO's concern that the 2030 targets of the WHO's global malaria strategy will not be met [2].Indeed, molecular markers of Plasmodium falciparum resistance to antimalarial drugs have been found to be associated with reduced treatment response and are useful in surveillance for resistance [5].Thus, among the tools to be developed and prioritized to combat malaria, more effective antimalarial medicines with new potential mechanisms of action are urgently needed [6].
In this context, efforts to discover new 4-aminoquinoline derivatives are ongoing.Indeed, it is unlikely that the parasite will be able to evolve resistance to drugs targeting the pathway involved in hemoglobin degradation.Previous studies showed that modulation and modification of the lateral side chain of chloroquine (CQ) or of mefloquine (MQ) that led to original aminoquinoline compounds avoid the CQ resistance mechanism [7][8][9][10].Another original strategy is to design and synthesize quinoline-based drugs that are not recognized by the protein system involved in the drug efflux.The efflux pumps serve both as natural defense mechanisms and influence the bioavailability and disposition of drugs.Initially, such mechanism was suggested in Plasmodium falciparum where erythrocytes infected with CQ-resistant parasites accumulated significantly less drug than the sensitive ones [11], before its further study led to the identification of the Pfcrt gene [12] among other quinoline drug resistance mechanisms [13].Such new series not recognized by the protein system involved in the drug efflux of bisquinoline and bisacridine antimalarial drugs (Figure 1A,B and Piperaquine) have been described in the literature [9,[14][15][16].These new derivatives had much lower resistance indices than CQ, indicating that these bis-heterocyclic structures are less efficiently rejected by efflux by drug-resistant parasites.Moreover, previous studies on in vitro antiplasmodial activity of diaza phenanthrene analogs indicated that the 1,10-phenanthroline skeleton represents an interesting and potential antimalarial lead compound.A few bioactive antimalarial phenanthrolines based on this skeleton have been described such as 4,7-phenanthrolines Figure 1C,D [17], 4-chloro-2-methyl-3-vinyl-1,10-phenanthroline Figure 1E [18], 4-methoxy-11-methyl-3Hpyrrolo [3,2-c] [1,10]phenanthroline Figure 1F [19], and N-benzyl-1,10-phenanthrolinium iodide G (Figure 1) [20,21].Moreover, the study of the trypanocidal activity of new anilinophenanthrolines Figure 1H,J against Trypanosoma cruzi has also been reported [22].Nevertheless, among the protozoa of medical interest, trypanosomatid parasites are also important, and responsible for tropical diseases, mainly leishmaniases caused by more than 20 Leishmania species described to be infective to humans, and trypanosomiases including human African trypanosomiasis (or sleeping sickness) caused by Trypanosoma brucei and South American trypanosomiasis (or Chagas disease) caused by Trypanosoma cruzi.Classified as neglected tropical diseases according to the WHO, which wants to eliminate them as a public health problem by 2030 [23], their impact is even more important because of the current limited therapeutic arsenal which is highly toxic and subject to the development of increasingly drug-resistant parasite strains [24].
involved in the drug efflux.The efflux pumps serve both as natural defense mechanisms and influence the bioavailability and disposition of drugs.Initially, such mechanism was suggested in Plasmodium falciparum where erythrocytes infected with CQ-resistant parasites accumulated significantly less drug than the sensitive ones [11], before its further study led to the identification of the Pfcrt gene [12] among other quinoline drug resistance mechanisms [13].Such new series not recognized by the protein system involved in the drug efflux of bisquinoline and bisacridine antimalarial drugs (Figure 1A,B and Piperaquine) have been described in the literature [9,[14][15][16].These new derivatives had much lower resistance indices than CQ, indicating that these bis-heterocyclic structures are less efficiently rejected by efflux by drug-resistant parasites.Moreover, previous studies on in vitro antiplasmodial activity of diaza phenanthrene analogs indicated that the 1,10-phenanthroline skeleton represents an interesting and potential antimalarial lead compound.A few bioactive antimalarial phenanthrolines based on this skeleton have been described such as 4,7-phenanthrolines Figure 1C,D [17], 4-chloro-2-methyl-3-vinyl-1,10-phenanthroline Figure 1E [18], 4-methoxy-11-methyl-3Hpyrrolo [3,2-c] [1,10]phenanthroline Figure 1F [ 9], and N-benzyl-1,10-phenanthrolinium iodide G (Figure 1) [20,21].Moreover, the study of the trypanocidal activity of new anilinophenanthrolines Figure 1H,J against Trypanosoma cruzi has also been reported [22].Nevertheless, among the protozoa of medical interest, trypanosomatid parasites are also important, and responsible for tropical diseases, mainly leishmaniases caused by more than 20 Leishmania species described to be infective to humans, and trypanosomiases including human African trypanosomiasis (or sleeping sickness) caused by Trypanosoma brucei and South American trypanosomiasis (or Chagas disease) caused by Trypanosoma cruzi.Classified as neglected tropical diseases according to the WHO, which wants to eliminate them as a public health problem by 2030 [23], their impact is even more important because of the current limited therapeutic arsenal which is highly toxic and subject to the development of increasingly drug-resistant parasite strains [24].

During our work, which is focused on the discovery of new nitrogen heterocyclic derivatives that can be used in antiprotozoal chemotherapy [25][26][27][28][29][30], we previously designed and synthesized a series of substituted 2,9-bis[(substituted-aminomethyl)phenyl]-1,10-phenanthroline derivatives (series A; Figure 1) During our work, which is focused on the discovery of new nitrogen heterocyclic derivatives that can be used in antiprotozoal chemotherapy [25][26][27][28][29][30], we previously designed and synthesized a series of substituted 2,9-bis[(substituted-aminomethyl)phenyl]-1,10-phenanthroline derivatives (series A; Figure 1) designed as antimalarial candidates, which could bind to Plasmodium falciparum DNA G-quadruplexes [31] in order to bypass the resistance mechanisms developed by the parasites and based on efflux.By considering our experience in the field of the synthesis of new antiprotozoal heterocyclic compounds, we describe here the design and synthesis of new 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives salts 1 (Series B; Figure 1) that could be considered to be new bio-isoster analogs of our previously described phenanthrolines series A. We report on their in vitro antiplasmodial activity against the CQ-sensitive (3D7) and the CQ-resistant (W2) strains of the malaria parasite P. falciparum.As we could consider that nitrogen heterocyclic pharmacophores are the fundamental moieties of several antiprotozoan candidates, these quinoline-like analogues were also tested for in vitro efficacy against medically important protozoans Leishmania donovani and Trypanosoma brucei brucei.

which co
ld bind to Plasmodium falciparum DNA G-quadruplexes [31] in order to bypass the resistance mechanisms developed by the parasites and based on efflux.By considering our experience in the field of the synthesis of new antiprotozoal heterocyclic compounds, we describe here the design and synthesis of new 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives salts 1 (Series B; Figure 1) that could be considered to be new bio-isoster analogs of our previously described phenanthrolines series A. We report on their in vitro antiplasmodial activity against the CQ-sensitive (3D7) and the CQ-resistant (W2) strains of the malaria parasite P. falciparum.As we could consider that nitrogen heterocyclic pharmacophores are the fundamental moieties of several antiprotozoan candidates, these quinoline-like analogues were also tested for in vitro efficacy against medically important protozoans Leishmania donovani and Trypanosoma brucei brucei.

In addition, the in vitro cytotoxicity of our new 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives 1 was assessed in human HepG2 cells, and a selectivity index, the ratio of cytotoxic to antiparasitic activity, was determined for each derivative.The telomeres of In addition, the in vitro cytotoxicity of our new 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives 1 was assessed in human HepG2 cells, and a selectivity index, the ratio of cytotoxic to antiparasitic activity, was determined for each derivative.The telomeres of the different protozoa could constitute attractive drug targets [32][33][34][35] in order to bypass the resistance mechanisms based on efflux and telomerase activity is detected in gametocytes and during the transition to the erythrocytic stage of P. falciparum [36].The telomeric 3 G-overhang region of P. falciparum is a repetition of degenerate unit 5 GGGTTYA3 (where Y could be T or C) [37] which can fold into intramolecular G-quadruplex [38].This difference between parasitic and human (5 GGGTTA3 ) G-quadruplexes is also observed with L. spp and T. brucei brucei, which augurs the possibility of developing antiparasitic ligands targeting specifically G-quadruplexes found in these protozoal species.Thus, we investigated whether these derivatives could stabilize some parasitic telomeric DNA G-quadruplex structures.Consequently, potential stabilization of P. falciparum and T. brucei brucei telomeric G-quadruplexes was evaluated using a FRET melting assay and by native mass spectrometry.
the different protozoa could constitute attractive drug targets [32][33][34][35] in order to bypass the resistance mechanisms based on efflux and telomerase activity is detected in gametocytes and during the transition to the erythrocytic stage of P. falciparum [36].The telomeric 3 G-overhang region of P. falciparum is a repetition of degenerate unit 5 GGGTTYA3 (where Y could be T or C) [37] which can fold into intramolecular G-quadruplex [38].This difference between parasitic and human (5 GGGTTA3 ) G-quadruplexes is also observed with L. spp and T. brucei brucei, which augurs the possibility of developing antiparasitic ligands targeting specifically G-quadruplexes found in these protozoal species.Thus, we investigated whether these derivatives could stabilize some parasitic telomeric DNA G-quadruplex structures.Consequently, potential stabilization of P. falciparum and T. brucei brucei telomeric G-quadruplexes was evaluated using a FRET melting assay and by native mass spectrometry.


Materials and Methods


Chemistry


General

Commercially available reagents were used as received without additional purification.Melting points were determined with an SM-LUX-POL Leitz hot-stage microscope (Leitz GMBH, Midland, ON, USA) and are uncorrected.NMR spectra were recorded with tetramethylsilane as an internal standard using a BRUKER AVANCE 300 spectrometer (Bruker BioSpin, Wissembourg, France).Splitting patterns have been reported as follows: s = singlet; bs = broad singlet; d = doublet; t = triplet; q = quartet; dd = double doublet; ddd = double double doublet; qt = quintuplet; m = multiplet.Ana

General
Commercially available reagents were used as received without additional purification.Melting points were determined with an SM-LUX-POL Leitz hot-stage microscope (Leitz GMBH, Midland, ON, USA) and are uncorrected.NMR spectra were recorded with tetramethylsilane as an internal standard using a BRUKER AVANCE 300 spectrometer (Bruker BioSpin, Wissembourg, France).Splitting patterns have been reported as follows: s = singlet; bs = broad singlet; d = doublet; t = triplet; q = quartet; dd = double doublet; ddd = double double doublet; qt = quintuplet; m = multiplet.Analytical TLC were carried out on 0.25 precoated silica gel plates (POLYGRAM SIL G/UV254) and visualization of compounds after UV light irradiation.Silica gel 60 (70-230 mesh) was used for column chromatography.Mass spectra were recorded on an ESI LTQ Orbitrap Velos mass spectrometer (ThermoFisher, Bremen, Germany).Ionization was performed using an Electrospray ion source operating in positive ion mode with a capillary voltage of 3.80 kV and capillary temperature of 250 • C. The scan type analyzed was full scan, all MS recordings were in the m/z range between 150 and 2000 m/z.None type of fragmentation was carried out and the resolution used for the analysis was 60.000.

General Procedure for the Synthesis of 2,9-Bis[(substituted-aminomethyl)phenyl]-4,7-diphenyl-1,10-phenanthrolines 4a-p
To a solution of compound 3a-p (0.4 mmol) in methanol (10 mL), sodium borohydride was added portion-wise at 0 • C (2.4 mmol, 6 eq.).The reaction mixture was then stirred at room temperature for 2 h.Then it was evaporated to dryness under reduced pressure.After cooling, the residue was triturated in water and extracted with dichloromethane (40 mL).The organic layer was separated, dried over sodium sulfate and activated charcoal, and evaporated to dryness.The residue was then purified by column chromatography on silica gel using dichloromethane/methanol (90/10: v/v) as eluent to give the pure products 4a-p.

Biological Evaluation 2.2.1. In Vitro Antiplasmodial Activity
The in vitro antiplasmodial activities were tested over concentrations ranging from 39 nM to 40 µM against culture-adapted Plasmodium falciparum reference strains 3D7 and W2.The former strain is susceptible to chloroquine (CQ) but displays a decreased susceptibility to mefloquine (MQ); the latter is considered to be resistant to CQ.These two strains are obtained from the collection of the National Museum of Natural History (Paris, France).The parasites were cultivated in RPMI medium (Sigma Aldrich, Lyon, France) supplemented with 0.5% Albumax I (Life Technologies Corporation, Paisley, UK), hypoxanthine (Sigma Aldrich), and gentamicin (Sigma Aldrich) with human erythrocytes and were incubated at 37 • C in a candle jar, as described previously [39].The P. falciparum drug susceptibility test was carried out in 96-well flat bottom sterile plates in a final volume of 250 µL.After 48 h incubation period with the drugs, quantities of DNA in treated and control cultures of parasites in human erythrocytes were quantified using the SYBR Green I (Sigma Aldrich) fluorescence-based method [40,41].Briefly, after incubation, plates were frozen at −20 • C until use.Plates were then thawed for 2 h at room temperature, and 100 µL of each homogenized culture was transferred to a well of a 96-well flat bottom sterile black plate (Nunc, Inc.) that contained 100 µL of the SYBR Green I lysis buffer (2xSYBR Green, 20 mM Tris base pH 7.5, 5 mM EDTA, 0.008% w/v saponin, 0.08% w/v Triton X-100).Negative controls treated with solvent (typically DMSO or H 2 O), and positive controls (CQ and MQ) were added to each set of experiments.Plates were incubated for 1 h at room temperature and then read on a fluorescence plate reader (Tecan, Austria) using excitation and emission wavelengths of 485 and 535 nm, respectively.The concentrations at which the screening drug or antimalarial is capable of inhibiting 50% of parasitic growth (IC 50 ) are calculated from a sigmoid inhibition model Emax with an estimate of IC 50 by non-linear regression (IC Estimator version 1.2) and are reported as means calculated from three independent experiments [42].

ma Aldrich, Lyon, France
supplemented with 0.5% Albumax I (Life Technologies Corporation, Paisley, UK), hypoxanthine (Sigma Aldrich), and gentamicin (Sigma Aldrich) with human erythrocytes and were incubated at 37 • C in a candle jar, as described previously [39].The P. falciparum drug susceptibility test was carried out in 96-well flat bottom sterile plates in a final volume of 250 µL.After 48 h incubation period with the drugs, quantities of DNA in treated and control cultures of parasites in human erythrocytes were quantified using the SYBR Green I (Sigma Aldrich) fluorescence-based method [40,41].Briefly, after incubation, plates were frozen at −20 • C until use.Plates were then thawed for 2 h at room temperature, and 100 µL of each homogenized culture was transferred to a well of a 96-well flat bottom sterile black plate (Nunc, Inc.) that contained 100 µL of the SYBR Green I lysis buffer (2xSYBR Green, 20 mM Tris base pH 7.5, 5 mM EDTA, 0.008% w/v saponin, 0.08% w/v Triton X-100).Negative controls treated with solvent (typically DMSO or H 2 O), and positive controls (CQ and MQ) were added to each set of experiments.Plates were incubated for 1 h at room temperature and then read on a fluorescence plate reader (Tecan, Austria) using excitation and emission wavelengths of 485 and 535 nm, respectively.The concentrations at which the screening drug or antimalarial is capable of inhibiting 50% of parasitic growth (IC 50 ) are calculated from a sigmoid inhibition model Emax with an estimate of IC 50 by non-linear regression (IC Estimator version 1.2) and are reported as means calculated from three independent experiments [42].


In Vitro Antileishmanial Activity

L. donovani (MHOM/IN/00/DEVI) used in this study was provided by the CNR Leishmania (Montpellier, France).The effects of the tested compounds on the growth of L. donovani (MHOM/IN/00/DEVI) promastigotes were assessed by MTT assay [43].Briefly, promastigo

In Vitro Antileishmanial Activity
L. donovani (MHOM/IN/00/DEVI) used in this study was provided by the CNR Leishmania (Montpellier, France).The effects of the tested compounds on the growth of L. donovani (MHOM/IN/00/DEVI) promastigotes were assessed by MTT assay [43].Briefly, promastigotes in log-phase in Schneider's medium supplemented with 20% fetal calf serum (FCS), 2 mM L-glutamine and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin), were incubated at an average density of 10 6 parasites/mL in sterile 96-well plates with various concentrations of compounds previously dissolved in DMSO (final concentration less than 0.5% v/v), in duplicate.Appropriate controls treated with DMSO, pentamidine, and amphotericin B (reference drugs purchased from Sigma Aldrich) were added to each set of experiments.Duplicate assays were performed for each sample.After the 72 h incubation period at 27 • C, parasite metabolic activity was determined.Each well was microscopically examined for precipitate formation.To each well was added 10 µL of 10 mg/mL MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] solution followed by 4 h incubation time.The enzyme reaction was stopped by addition of 100 µL of 50% isopropanol/10% sodium dodecyl sulfate [44].Plates were vigorously shaken (300 rpm) for 10 min, and the absorbance was measured at 570 nm with 630 nm as reference wavelength in a BIOTEK ELx808 Absorbance Microplate Reader (Agilent Technologies, Les Ulis, France).The IC 50 was defined as the concentration of drug required to inhibit by 50% of the metabolic activity of L. donovani promastigotes compared to the control.IC 50 of the parasite's growth (half maximal inhibitory concentration or IC 50 values) were then calculated from the obtained experimental results using a previously described regression program [42].IC 50 values were calculated from three independent experiments.

s in log-phase in Schneid
r's medium supplemented with 20% fetal calf serum (FCS), 2 mM L-glutamine and antibiotics (100 U/mL penicillin and 100 µg/mL streptomycin), were incubated at an

verage density of 10 6 parasites/mL in
sterile 96-well plates with various concentrations of compounds previously dissolved in DMSO (final concentration less than 0.5% v/v), in duplicate.Appropriate controls treated with DMSO, pentamidine, and amphotericin B (reference drugs purchased from Sigma Aldrich) were added to each set of experiments.Duplicate assays were performed for each sample.After the 72 h incubation period at 27 • C, parasite metabolic activity was determined.Each well was microscopically examined for precipitate formation.To each well was added 10 µL of 10 g/mL MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)] solution followed by 4 h incubation time.The enzyme reaction was stopped by addition of 100 µL of 50% isopropanol/10% sodium dodecyl sulfate [44].Plates were vigorously shaken (300 rpm) for 10 min, and the absorbance was measured at 570 nm with 630 nm as reference wavelength in a BIOTEK ELx808 Absorbance Microplate Reader (Agilent Technologies, L

Ulis, France).The
C 50 was defined as the concentration of drug required to inhibit by 50% of the metabolic activity of L. donovani promastigotes compared to the control.IC 50 of the parasite's growth (half maximal inhibitory concentration or IC 50 values) were then calculated from the obtained experimental results using a previously described regression program [42].IC 50 values were calculated from three independent experiments.


In Vitro Antitrypanosomal Ac

vity

Th
The effects of the tested compounds on the growth of T. brucei brucei were assessed using an Alamar Blue ® assay described by Räz et al. [45] T. brucei brucei AnTat 1.9 (IMTA, Antwerpen, Belgium) was cultured in MEM with Earle's salts, supplemented according to the protocol of Baltz et al. [46] with the following modifications: 0.5 mM mercaptoethanol (Sigma Aldrich), 1.5 mM L-cysteine (Sigma Aldrich), 0.05 mM bathocuproine sulfate (Sigma Aldrich), and 20% heat-inactivated horse serum (Gibco, France) at 37 • C and 5% CO 2 .Samples were incubated at an average density of 2000 parasites/well in sterile 96-wells plates (Fisher, France) with various concentrations of compounds dissolved in 0.9% NaCl.All doses were tested in duplicate.Appropriate controls treated with solvents 0.9% NaCl or DMSO or with suramin, pentamidine, eflornithine, and fexinidazole (reference drugs purchased from Sigma Aldrich and Fluorochem, UK) were added to each set of experiments.After 69 h incubation period at 37 • C, 10 µL of the viability marker Alamar Blue (Fisher) was added to each well, and the plates were incubated for 5 h.The plates were read in a PerkinElmer ENSPIRE (Germany) microplate reader using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.The IC 50 was defined as the concentration of drug necessary to inhibit by 50% the activity of T. brucei brucei compared to the control.IC 50 values were calculated using a nonlinear regression analysis of dose-response curves performed using GraphPad Prism software (GraphPad Software, San Diego, CA, USA).IC 50 values were calculated from three independent experiments.

effects of
the tested compounds on the growth of T. brucei brucei were assessed using an Alamar Blue ® assay described by Räz et al. [45] T. brucei brucei AnTat 1.9 (IMTA, Antwerpen, Belgium) was cultured in MEM with Earle's salts, supplemented according to the protocol of Baltz et al. [46] with the following modifications: 0.5 mM mercaptoethanol (Sigma Aldrich), 1.5 mM L-cysteine (Sigma Aldrich), 0.05 mM bathocuproine sulfate (Sigma Aldrich), and 20% heat-inactivated horse serum (Gibco, France) at 37 • C and 5% CO 2 .Samples were incubated at an average density of 2000 parasites/well in sterile 96-wells plates (Fisher, France) with various concentrations of compounds dissolved in 0.9% NaCl.All doses were tested in duplicate.Appropriate controls treated with solvents 0.9% NaCl or DMSO or with suramin, pentamidine, eflornithine, and fexinidazole (reference drugs purchased from Sigma Aldrich and Fluorochem, UK) were added to each set of experiments.After 69 h incubation per

e viability marker Alamar Blue
Fisher) was added to each well, and the plates were incubated for 5 h.The plates were read in a PerkinElmer ENSPIRE (Germany) microplate reader using an excitation wavelength of 530 nm and an emission wavelength of 590 nm.The IC 50 was defined as the concentration of drug necessary to inhibit by 50% the activity of T. brucei brucei compared to the control.IC 50 values were calculated using a nonlinear regression analysis of dose-response curves performed using GraphPad Prism software (GraphPad Software, San Die o, CA, USA).IC 50 values were calculated from three independent experiments.


Cytotoxicity Evaluation

A cytotoxicity evaluation was performed using the method reported by Mosmann [43] with slight modifications to determine the cytotoxic concentrations 50% (CC 50 ) and using doxorubicin as a cytotoxic reference compound.These assays were performed in human HepG2 cells purchased from ATCC (ref HB-8065).These cells are a commonly used human hepatocarcinoma-derived cell line that has characteristics similar to those of primary hepatocytes.These cells express many hepatocyte-specific metabolic enzymes, thus enabling the cytotoxicity of tested product metabolites to be evaluated.Briefly, cells in 100 µL of complete RPMI medium, (RPMI supplemented with 10% FCS, 1% L-glutamine (200 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL)) were inoculated at 37 • C into each well of 96-well plates in a humidified chamber in 6% CO 2 .After 24 h, 100 µL of medium with test compound at various concentrations dissolved in DMSO (final concentration less than 0.5% v/v) were added, and the plates were incubated for 72 h at 37 • C. Duplicate assays were performed for each sample.Each well was microscopically examined for precipitate formation before the medium was aspirated from the wells.After aspiration, 100 µL of MTT solution (0.5 mg/mL in medium without FCS) were then added to each well.Cells were incubated for 2 h at 37 • C. The MTT solution was removed, and DMSO (100 µL) was added to dissolve the resulting blue formazan crystals.Plates were shaken vigorously (300 rpm) for 5 min.The absorbance was measured at 570 nm with 630 nm as reference wavelength in a BIO-TEK ELx808 Absorbance Microplate Reader.DMSO was used as blank and doxorubicin (Sigma Aldrich) as positive control.Cell viability was calculated as percentage of control (cells incubated without compound).The CC 50 was determined from the dose-response curve using TableCurve 2D V5.0 software (Systat Software, Palo Alto, CA, USA).


FRET Melting Experiments

The bioactive compounds 1a-p were selected for the subsequent FRET melting experiments.These were performed with dual-labeled oligonucleotides mimicking the Plasmodium telomeric sequences FPf1T [FAM-5 (GGGTTTA)3-GGG3 -TAMRA] and FPf8T [FAM-5 (GGGTTCA)3GGG3 -TAMRA], the Trypanosoma 9 and 11 chromosomic sequence FTrypBT (also named FEBR1T) [FAM-5 GGGCAGGGGGTGATGGGGAGGAGCCAGGG3 -TAMRA], the human telomeric sequence F21T [FAM-(GGGTTA)3-GGG3 -TAMRA] and the human duplex sequence FdxT [FAM5 -TATAGCTATA-hexaethyleneglycol-TATAGCTATA3 -TAMRA] [27, 31,47].The oligonucleotides were pre-folded in 10 mM lithium cacodylate buffer (pH 7.2), with 10 mM KCl and 90 mM LiCl (K + condition).The FAM emissions were recorded at 516 nm using a 492-nm excitation wavelength in the absence and presence of a single compound as a function of temperature (25 to 9

Cytotoxicity Evaluation
A cytotoxicity evaluation was performed using the method reported by Mosmann [43] with slight modifications to determine the cytotoxic concentrations 50% (CC 50 ) and using doxorubicin as a cytotoxic reference compound.These assays were performed in human HepG2 cells purchased from ATCC (ref HB-8065).These cells are a commonly used human hepatocarcinoma-derived cell line that has characteristics similar to those of primary hepatocytes.These cells express many hepatocyte-specific metabolic enzymes, thus enabling the cytotoxicity of tested product metabolites to be evaluated.Briefly, cells in 100 µL of complete RPMI medium, (RPMI supplemented with 10% FCS, 1% L-glutamine (200 mM), penicillin (100 U/mL), and streptomycin (100 µg/mL)) were inoculated at 37 • C into each well of 96-well plates in a humidified chamber in 6% CO 2 .After 24 h, 100 µL of medium with test compound at various concentrations dissolved in DMSO (final concentration less than 0.5% v/v) were added, and the plates were incubated for 72 h at 37 • C. Duplicate assays were performed for each sample.Each well was microscopically examined for precipitate formation before the medium was aspirated from the wells.After aspiration, 100 µL of MTT solution (0.5 mg/mL in medium without FCS) were then added to each well.Cells were incubated for 2 h at 37 • C. The MTT solution was removed, and DMSO (100 µL) was added to dissolve the resulting blue formazan crystals.Plates were shaken vigorously (300 rpm) for 5 min.The absorbance was measured at 570 nm with 630 nm as reference wavelength in a BIO-TEK ELx808 Absorbance Microplate Reader.DMSO was used as blank and doxorubicin (Sigma Aldrich) as positive control.Cell viability was calculated as percentage of control (cells incubated without compound).The CC 50 was determined from the dose-response curve using TableCurve 2D V5.0 software (Systat Software, Palo Alto, CA, USA).

FRET Melting Experiments
The bioactive compounds 1a-p were selected for the subsequent FRET melting experiments.These were performed with dual-labeled oligonucleotides mimicking the Plasmodium telomeric sequences FPf1T [FAM-5 (GGGTTTA)3-GGG3 -TAMRA] and FPf8T [FAM-5 (GGGTTCA)3GGG3 -TAMRA], the Trypanosoma 9 and 11 chromosomic sequence FTrypBT (also named FEBR1T) [FAM-5 GGGCAGGGGGTGATGGGGAGGAGCCAGGG3 -TAMRA], the human telomeric sequence F21T [FAM-(GGGTTA)3-GGG3 -TAMRA] and the human duplex sequence FdxT [FAM5 -TATAGCTATA-hexaethyleneglycol-TATAGCTATA3 -TAMRA] [27, 31,47].The oligonucleotides were pre-folded in 10 mM lithium cacodylate buffer (pH 7.2), with 10 mM KCl and 90 mM LiCl (K + condition).The FAM emissions were recorded at 516 nm using a 492-nm excitation wavelength in the absence and presence of a single compound as a function of temperature (25 to 95 • C) in 96-well microplates using a Stratagene MX3000P real-time PCR device at a rate of 1 • C•min-1.Data were normalized between 0 and 1, and the required temperature for half-denaturation of oligonucleotides corresponding to the emission value of 0.5 was taken as the Tm.Each experiment was performed in duplicate with 0.2 µM of labeled oligonucleotide and 2 µM of compound under K + condition.For each compound, three independent experiments were carried out.

in 96-well microplates using a Stratagene MX3000P real-time P
R device at a rate of 1 • C•min-1.Data were normalized between 0 and 1, and the required temperature for half-denaturation of oligonucleotides corresponding to the emission value of 0.5 was taken as the Tm.Each experiment was performed in duplicate with 0.2 µM of labeled oligonucleotide and 2 µM of compound under K + condition.For each compound, three independent experiments were carried out.


Native Electrospray Mass Spectrometry and Circular Dichroism 2.4.1. Samples

Oligonucleotides were purchased in the lyophilized form from Eurogentec (RPcartridge purification), and dissolved at 1 mM in pure water.TMAA (1 M) was purchased from Santa Cruz Biotechnology and potassium chloride (purity > 99.99%) from Sigma Aldrich (Sigma Aldrich, Saint-Quentin-Fallavier, France).The sample solutions contained 10 µM oligonucleotide and 10 (for CD only) or 20 µM ligand, in a 100 mM TMAA, 1mM KCl buffer.The solutions were incubated overnight at 4 • C before analysis.


Native Mass Electrospray Sp

Native Electrospray Mass Spectrometry and Circular Dichroism 2.4.1. Samples
Oligonucleotides were purchased in the lyophilized form from Eurogentec (RPcartridge purification), and dissolved at 1 mM in pure water.TMAA (1 M) was purchased from Santa Cruz Biotechnology and potassium chloride (purity > 99.99%) from Sigma Aldrich (Sigma Aldrich, Saint-Quentin-Fallavier, France).The sample solutions contained 10 µM oligonucleotide and 10 (for CD only) or 20 µM ligand, in a 100 mM TMAA, 1mM KCl buffer.The solutions were incubated overnight at 4 • C before analysis.

trometry

The sample solutions were injected at a 10
The sample solutions were injected at a 10 µM strand concentration on an Agilent 6560 DTIMS-Q-TOF instrument (Agilent Technologies, Santa Clara, CA, USA), equipped with a dual-ESI source operated in the negative ion mode in soft conditions [48].The following parameters were used: fragmentor 320 V, trap fill time 1000 µs, trap release time 200 µs, trap entrance grid delta 2 V.The drift tube was filled with helium and the pressure was fixed at 3.89 ± 0.01 Torr (measured by a capacitance diaphragm gauge CDG-500, Agilent Technologies).
µM strand concentration on an Agilent 6560 DTIMS-Q-TOF instrument (Agilent Technologies, Santa Clara, CA, USA), equipped with a dual-ESI source operated in the negative ion mode in soft conditions [48].The following parameters were used: fragmentor 320 V, trap fill time 1000 µs, trap release time 200 µs, trap entrance grid delta 2 V.The drift tube was filled with helium and the pressure was fixed at 3.89 ± 0.01 Torr (measured by a capacitance diaphragm gauge CDG-500, Agilent Technologies).

The MS data were extracted using the IM-MS Browser (v.B.08.00; Agilent Technologies).The peak area intensity of each species at the 5-charge state was extracted from their corresponding m/z range (encompassing potassium adducts), and baseline subtracted.The concentrations of all species (free DNA, free ligands, and complexes) and corresponding dissociation constants were determined as previously described [49,50].


Circular Dichroism

CD experiments were performed with a JASCO J-1500 spectropolarimeter using quartz cells of 2 mm path length.The scans were recorded at 22 • C, from 220 to 350 nm with the fo The MS data were extracted using the IM-MS Browser (v.B.08.00; Agilent Technologies).The peak area intensity of each species at the 5-charge state was extracted from their corresponding m/z range (encompassing potassium adducts), and baseline subtracted.The concentrations of all species (free DNA, free ligands, and complexes) and corresponding dissociation constants were determined as previously described [49,50].

Circular Dichroism
CD experiments were performed with a JASCO J-1500 spectropolarimeter using quartz cells of 2 mm path length.The scans were recorded at 22 • C, from 220 to 350 nm with the following parameters: 1.0 nm data pitch, 2 nm bandwidth, 0.5 s response, 50 nm/min scanning speed; they are the result of three accumulations.The CD data were blank-subtracted, baseline subtracted and normalized to molar dichroic absorption as previously described [49,50].

In Vitro Antimalarial Activity
All the new phenanthroline derivatives 1a-p were evaluated for their antimalarial activity in vitro by incubation with P. falciparum CQ-resistant strain W2 (IC 50 CQ = 0.40 µM, IC 50 MQ = 0.016 µM) and the strain 3D7, which is CQ-sensitive and which has decreased sensitivity to MQ (IC 50 CQ = 0.11 µM, MQ = 0.06 µM) (Table 2).As shown in Table 2, these new phenanthroline derivatives 1 showed IC 50 values between 0.03 and 11.27 µM against W2, and between 0.47 and 21.19 µM against the 3D7 P. falciparum strains.

d between 0.47 and 21.19
M against the 3D7 P. falciparum strains.

In terms of structure-activities relationships, the influence of the length of the carbon chain between the two amino functions of the side chain, and also the nature of the terminal amine group, were discussed.Against the P. falciparum CQ-resistant strain W2, compounds 1c, 1i and 1l bearing dimethylaminobutylaminomethyl, morpholinopropylaminomethyl, or pyrrolidinopropylaminomethyl side chains at positions 2 and 9 of the phenanthroline moiety were found to be the most active compounds with an IC 50 of 0.03, 0.04 and 0.03 µM, respectively, in the same range as the one observed for mefloquine (IC 50 = 0.016 µM).Concerning these phenanthrolines substituted by aminoethylaminomethyl side chains, derivative 1h bearing morpholinoethylaminomethyl s In terms of structure-activities relationships, the influence of the length of the carbon chain between the two amino functions of the side chain, and also the nature of the terminal amine group, were discussed.Against the P. falciparum CQ-resistant strain W2, compounds 1c, 1i and 1l bearing dimethylaminobutylaminomethyl, morpholinopropylaminomethyl, or pyrrolidinopropylaminomethyl side chains at positions 2 and 9 of the phenanthroline moiety were found to be the most active compounds with an IC 50 of 0.03, 0.04 and 0.03 µM, respectively, in the same range as the one observed for mefloquine (IC 50 = 0.016 µM).Concerning these phenanthrolines substituted by aminoethylaminomethyl side chains, derivative 1h bearing morpholinoethylaminomethyl side chains displayed better activity than its three other analogues (IC 50 = 0.17 µM for 1h versus IC 50 = 0.92-2.60µM for 1a, 1e and 1j).In comparison, when we replaced the aminoethylaminomethyl side functions by aminopropylaminomethyl side chains (compounds 1b, 1f, 1i, 1k and 1l), the disubstituted phenanthrolines 1i and 1l bearing morpholino C3 aminomethyl or pyrrolidino C3 aminomethyl side chains showed the best antimalarial activity, i.e., IC 50 = 0.04 µM for 1i and 0.03 µM for 1l versus 11.27 µM for their analog 1f bearing methylpiperazinyl C3 aminomethyl side chains).In the sub-series of the phenanthrolines 1a-d substituted by dimethylaminoalkylaminomethyl side chains, compound 1c with butyl chains exhibited a better antimalarial activity than its di-substituted ethyl, propyl, or pentyl analogues 1a-b and 1d (IC 50 = 0.03 µM for 1c versus IC 50 = 1.00-6.13µM for the other ones).In the pyridinylethylaminomethyl substituted bisphenanthroline series (compounds 1m-o), derivative 1m containing (pyridin-2-yl)ethyl)aminomethyl chains was found more active (up to 36.3 or 26.7 times) against the P. falciparum W2 strain than its counterpart compounds 1n and 1o with (pyridin-3-yl)ethyl)aminomethyl or (pyridin-4-yl)ethyl)aminomethyl chains (IC 50 = 0.07 µM for 1m versus IC 50 = 2.54 and 1.87 µM for 1n and 1o).Moreover, the substitution by (pyridin-4-yl)propyl)aminomethyl chains side chains (compound 1p) showed a better antimalarial activity against the W2 strain than its homologue substituted at positions 2 and 9 by (pyridin-4-yl)ethyl)aminomethyl chains (compound 1o); i.e., IC 50 = 0.145 µM for 1p versus 1.87 µM for 1o. a Values were measured against CQ-resistant and mefloquine-sensitive strain W2 and the CQ-sensitive and MQ-resistant strain 3D7.b IC 50 values were measured against the promastigotes of Leishmania donovani strain.The IC 50 (µM) values correspond to the means +/− standard deviations from three independent experiments.c IC 50 values were measured against the slender bloodstream trypomastigotes of Trypanosoma brucei brucei AnTat 1.9 strain.The IC 50 (µM) values correspond to the means +/− standard deviations from three independent experiments with each concentration tested in duplicate in all experiments.d CC 50 values were measured against HepG2 cells.The CC 50 (µM) values correspond to the means +/− standard deviations from three independent experiments.e CQ and MQ were used as antiplasmodial compounds of reference.f Pentamidine and amphotericin B were used as antileishmanial compounds of reference.g Suramine, pentamidine, fexinidazole, and eflornithine were used as antitrypanosomal compounds of reference.h n.d.: not determined.i Molecule was not tested at higher concentrations (not active).

compounds of reference.h n.d.: not determined.i Molecule was
not tested at higher concentrations (not active).

Among the newly reported di-substituted(aminoethylaminomethyl)phenanthroline derivatives 1, compound 1h with morpholinoethylaminomethyl side chains exhibited the most potent activity against the 3D7 strain with an IC 50 of 0.67 µM but slightly more active than those observed for the other bis-aminoethylaminomethyl substituted compounds 1a, 1e, and 1j (IC 50 = 1.59-2.16µM).However, this phenanthroline derivative 1h was noticed less active than the two reference drugs, CQ and MQ, which had IC 50 values of 0.11 and 0.06 µM, respectively.Moreover, the influence of the length of the carbon chain in the aminoalkylaminomethyl side chains for this phenanthroline series seems also to be detrimental: a longer alkyl chain (aminopropylamine chain to aminopentylamine chain) in comparison with an aminoethylamine chain led to a decreased in the antiplasmodial activity and this whatever the terminal amine function (dimethylamine or methylpiperazine moities) of the side chain, (for example; IC 50 = 1.80 µM for 1a vs. 21.19,10.31 and 4.79 µM for 1b, 1c, and 1d, respectively).In the pyridinylethylaminomethyl substituted bisphenanthroline series (compounds 1m-o), whatever the position of the pyridine moiety on the pyridinylethylaminomethyl side chain, compounds 1m-o showed similar range of antiparasitic activities against the 3D7 strain (IC 50 = 0.47-0.73µM).


In Vitro Antileishmanial Activity against Promastigote Forms

To better understand the pharmaco Among the newly reported di-substituted(aminoethylaminomethyl)phenanthroline derivatives 1, compound 1h with morpholinoethylaminomethyl side chains exhibited the most potent activity against the 3D7 strain with an IC 50 of 0.67 µM but slightly more active than those observed for the other bis-aminoethylaminomethyl substituted compounds 1a, 1e, and 1j (IC 50 = 1.59-2.16µM).However, this phenanthroline derivative 1h was noticed less active than the two reference drugs, CQ and MQ, which had IC 50 values of 0.11 and 0.06 µM, respectively.Moreover, the influence of the length of the carbon chain in the aminoalkylaminomethyl side chains for this phenanthroline series seems also to be detrimental: a longer alkyl chain (aminopropylamine chain to aminopentylamine chain) in comparison with an aminoethylamine chain led to a decreased in the antiplasmodial activity and this whatever the terminal amine function (dimethylamine or methylpiperazine moities) of the side chain, (for example; IC 50 = 1.80 µM for 1a vs. 21.19,10.31 and 4.79 µM for 1b, 1c, and 1d, respectively).In the pyridinylethylaminomethyl substituted bisphenanthroline series (compounds 1m-o), whatever the position of the pyridine moiety on the pyridinylethylaminomethyl side chain, compounds 1m-o showed similar range of antiparasitic activities against the 3D7 strain (IC 50 = 0.47-0.73µM).

In Vitro Antileishmanial Activity against Promastigote Forms
To better understand the pharmacological profile of our new phenanthroline heterocycles 1, some additional antiparasitic analyses were also performed.Notably, P. falciparum belongs to the coccidian protozoan parasite family.Therefore, in vitro activity against flagellate protozoan parasite L. donovani was evaluated (Table 2).The reference drugs amphotericin B and pentamidine had IC 50 values of 0.10 µM and 5.50 µM, respectively, against L. donovani.Only the phenanthrolines 1h, 1j, 1n and 1o were found active against the promastigote forms of L. donovani with IC 50 from 2.52 to 4.50 µM.These biological results show that the activity of these derivatives appears to be related to the presence of an ethyl group substituted by a nitrogen membrered heterocycle, such as morpholine, piperidine or pyridine, i.e., compounds 1h, 1j, 1n and 1o, respectively.Unfortunately, none of all of the other compounds 1 showed any anti-leishmanial activity in vitro (all IC 50 values > 12.5 µM).
ogical profile of our new phenanthroline heterocycles 1, some additional antiparasitic analyses were also performed.Notably, P. falciparum belongs to the coccidian protozoan parasite family.Therefore, in vitro activity against flagella e protozoan parasite L. donovani was evaluated (Table 2).The reference drugs amphotericin B and pentamidine had IC 50 values of 0.10 µM and 5.50 µM, respectively, against L. donovani.Only the phenanthrolines 1h, 1j, 1n and 1o were found active against the promastigote forms of L. donovani with IC 50 from 2.52 to 4.50 µM.These biological results show that the activity of these derivatives appears to be related to the presence of an ethyl group substituted by a nitrogen membrered heterocycle, such as morpholine, piperidine or pyridine, i.e., compounds 1h, 1j, 1n and 1o, respectively.Unfortunately, none of all of the other compounds 1 showed any anti-leishmanial activity in vitro (all IC 50 values > 12.5 µM).


In Vitro Activity against Trypanosoma Brucei Brucei

These novel synthesized aza heterocyclic compounds 1a-p were then evaluated against T. brucei brucei.In addition, pentamidine, suramine, fexinidazole, and eflornithine were used here as reference compounds.The screening data are presented in Table 2.All phenanthrolines 1a-p were active against T. brucei brucei with IC 50 values ranging from 0.20 to 1.42 µM; and most of them showed an antiparasitic activity around the 1 µM.In terms of structure-activities relationships, an increase of the alkyl chain in the aminoalkylaminomethyl side chains seems to show a slight decrease in the antitrypanosomal activity, i.e., IC 50 = 0.45 µM for 1e versus 0.97 and 0.96 µM for 1f and 1g, respectively.In the pyridinylethylaminomethyl substituted bisphenanthroline series (

In Vitro Activity against Trypanosoma Brucei Brucei
These novel synthesized aza heterocyclic compounds 1a-p were then evaluated against T. brucei brucei.In addition, pentamidine, suramine, fexinidazole, and eflornithine were used here as reference compounds.The screening data are presented in Table 2.All phenanthrolines 1a-p were active against T. brucei brucei with IC 50 values ranging from 0.20 to 1.42 µM; and most of them showed an antiparasitic activity around the 1 µM.In terms of structure-activities relationships, an increase of the alkyl chain in the aminoalkylaminomethyl side chains seems to show a slight decrease in the antitrypanosomal activity, i.e., IC 50 = 0.45 µM for 1e versus 0.97 and 0.96 µM for 1f and 1g, respectively.In the pyridinylethylaminomethyl substituted bisphenanthroline series (compounds 1m-o), phenanthroline 1m containing (pyridin-4-yl)ethyl)aminomethyl chains exhibited a better antitrypanosomal activity (IC 50 = 0.21 µM) than its homologues bearing (pyridin-2yl)ethyl)aminomethyl or (pyridin-3-yl)ethyl)aminomethyl side chains (compounds 1m and 1n) for which IC 50 were noticed at 0.63 and 0.43 µM, respectively.

Cytotoxicity and Selectivity Index
To assess selectivity of action, the cytotoxicities of these newly synthesized antiparasitic phenanthroline compounds 1 were evaluated in vitro in the human cell line HepG2 (Table 2), which is a commonly used human-derived hepatocarcinoma cell line, and express many hepatocyte-specific metabolic enzymes.The goal of this assay was to evaluate the impact of metabolic activation of the tested phenanthrolines on cell viability [52,53].The cytotoxic concentrations 50% (CC 50 ) were determined, and selectivity indexes (SI), defined as the ratios of cytotoxic to antiparasitic activities (SI = CC 50 /IC 50 ) were calculated.The results of cytotoxicity assays and the associated SI values are presented in Table 3.Most of these phenanthrolines 1 that were found active against the various parasites showed significant cytotoxicity against the HepG2 cells with CC 50 values ranging from 1.64 to 15.17 µM.Concerning the W2 strain, the calculated SIs were noticed between 0.76 and 505.7.For the CQ-sensitive strain 3D7, the SIs were found from 0.28 to 26.18.Analyses of these SI values led us to identify the phenanthrolines 1l and 1c as very interesting compounds with SI of 505.7 and 347.67 for the W2 strain, respectively.In addition, the derivative 1i also showed promising SI value of 157.25 for this CQ-resistant strain W2.In addition, this SI led to the identification of compound 1o with SI of 7.1 on L. donovani.Against the T. brucei brucei strain, 2,9-bis [2-(pyridin-4-yl)ethyl)aminomethyl]-4,7-diphenyl-1,10-phenanthroline 1o had SI of 91.0.These promising SI values could indicate that these new nitrogen heterocyclic compounds warrant further investigations into their potential use as antiparasitic drugs.
assay was to evaluate the impact of metabolic activation of the tested phenanthrolines on cell viability [52,53].The cytotoxic concentrations 50% (CC 50 ) were determined, and

lectivity in
exes (SI), defined as the ratios of cytotoxic to antiparasitic activities (SI = CC 50 /IC 50 ) were calculated.The results of cytotoxicity assays and the associated SI values are presented in Table 3.Most of these phenanthrolines 1 that were found active against the various parasites showed significant cytotoxicity against the HepG2 cells with CC 50 values ranging from 1.64 to 15.17 µM.Concerning the W2 strain, the calculated SIs were noticed between 0.76 and 505.7.For the CQ-sensitive strain 3D7, the SIs were found from 0.28 to 26.18.Analyses of these SI values led us to identify the phenanthrolines 1l and 1c as very interesting compounds with SI of 505.7 and 347.67 for the W2 strain, respectively.In addition, the derivative 1i also showed promising SI value of 157.25 for this CQ-resistant strain W2.In addition, this SI led to the identification of compound 1o with SI of 7.1 on L. donovani.Against the T. brucei brucei strain, 2,9-bis [2-(pyridin-4-yl)ethyl)aminomethyl]-4,7-diphenyl-1,10-phenanthroline 1o had SI of 91.0.These promising SI values could indicate that these new nitrogen heterocyclic compounds warrant further investigations into their potential use as antiparasitic drugs.


FRET Melting Experiments

As the telomeres of the parasites P. falciparum and Trypanosoma could be considered to be potential targets of this kind of nitrogen heterocyclic derivatives [31,[36][37][38], we have also investigated the stabilization of the P. falciparum telomeric or T. brucei brucei chromosomic G-quadruplexes by our pharmacological active derivatives 1 using a FRET melting assays.We used a FRET melting assay to determine the degree to which the new phenanthroline derivatives stabilize the G-quadruplexes formed by oligonucleotides with P. falciparum or T. brucei brucei as well as human telomeric sequences.For this purpose, we used two fluorescently labeled P. falciparum telomeric and one T. brucei brucei chromosomic sequences (FPf1T, FPf8T and FtrypBT) and one human telomeric sequence (F21T).

To probe the G4 selectivity of our ligands 1a-p over duplex DNA, a FRET melting assay was performed using a duplex control sequence, FdxT.For comparison, we evaluated reference G4 ligand PhenDC3 and the antimalarial reference drugs CQ and MQ.To enable comparison of selectivities, we calculated the difference (∆Tm) between the Tm of the G-quadruplex formed by FPf1T, FPf8T, FtrypBT (FEBR1T), F21T or FdxT in the presence or absence of each selected compound.These ∆Tm values are presented in Table 4.For these selected compounds 1a-p, the ∆Tm values ranged from 1.0 to 20.5 • C at 2 µM ligand concentration.

The stabilization of our compounds was also investigated against the fluorescently labeled human telomeric sequence F21T.To probe the G4 selectivity of our heterocyclic ligands 1 over duplex DNA, a FRET melting assay was performed using a duplex control sequence, FdxT (Table 4).

The best ligands which stabilize all the four G-quadruplexes sequences were compounds 1d, 1g and 1l (Table 4).The best di-nitrogen heterocyclic ligand which stabilize all the three parasitic (FPf1T, FPf8T, FtrypBT) and the human F21T G-quadruplexes sequences was the 2,9-bis[(5-dimethylaminopentyl)ami

FRET Melting Experiments
As the telomeres of the parasites P. falciparum and Trypanosoma could be considered to be potential targets of this kind of nitrogen heterocyclic derivatives [31,[36][37][38], we have also investigated the stabilization of the P. falciparum telomeric or T. brucei brucei chromosomic G-quadruplexes by our pharmacological active derivatives 1 using a FRET melting assays.We used a FRET melting assay to determine the degree to which the new phenanthroline derivatives stabilize the G-quadruplexes formed by oligonucleotides with P. falciparum or T. brucei brucei as well as human telomeric sequences.For this purpose, we used two fluorescently labeled P. falciparum telomeric and one T. brucei brucei chromosomic sequences (FPf1T, FPf8T and FtrypBT) and one human telomeric sequence (F21T).
To probe the G4 selectivity of our ligands 1a-p over duplex DNA, a FRET melting assay was performed using a duplex control sequence, FdxT.For comparison, we evaluated reference G4 ligand PhenDC3 and the antimalarial reference drugs CQ and MQ.To enable comparison of selectivities, we calculated the difference (∆Tm) between the Tm of the G-quadruplex formed by FPf1T, FPf8T, FtrypBT (FEBR1T), F21T or FdxT in the presence or absence of each selected compound.These ∆Tm values are presented in Table 4.For these selected compounds 1a-p, the ∆Tm values ranged from 1.0 to 20.5 • C at 2 µM ligand concentration.
The stabilization of our compounds was also investigated against the fluorescently labeled human telomeric sequence F21T.To probe the G4 selectivity of our heterocyclic ligands 1 over duplex DNA, a FRET melting assay was performed using a duplex control sequence, FdxT (Table 4).
The best ligands which stabilize all the four G-quadruplexes sequences were compounds 1d, 1g and 1l (Table 4).The best di-nitrogen heterocyclic ligand which stabilize all the three parasitic (FPf1T, FPf8T, FtrypBT) and the human F21T G-quadruplexes sequences was the 2,9-bis[(5-dimethylaminopentyl)aminomethyl]-4,7-diphenyl-1,10-phenanthroline 1d with ∆Tm values ranging from 14.7 to 20.5 • C. In each sub-series, it could be observed that the best phenanthroline heterocyclic compounds 1 which exhibited an interesting stabilization profile were those substituted by the longest aminoalkylaminomethyl side chains.For example, for each G-quadruplex sequence, the substituted diphenylphenanthro-line ligand 1d bearing (dimethylaminopentyl)aminomethyl side chains exhibited the best strong stabilization profile in comparison with their (dimethylaminobutyl)aminomethyl or (dimethylaminopropyl)aminomethyl or (dimethylaminoethyl)aminomethyl substituted homologues 1a-c; these latter being least stabilizing ligands.The same observations could also be noticed for derivative 1g versus 1e-f; 1i versus 1h; and also compound 1k versus 1j.Concerning the position of the substitution of the pyridine group on the pyridinylalkylaminomethyl chains (compounds 1m-p), we cannot really conclude in terms of structure-activity relationships.A radar plot shown in Figure 2 show that compounds 1d, 1g, and 1l are able to stabilize most of all the G4 forming sequences.From a general point of view, all our ligands 1a-p were found to show lower stabilization than the reference PhenDC3 ligand.FRET assays showed there was no binding to duplex DNA sequence.
omethyl]-4,7-diphenyl-1,10-phenanthroline 1d with ∆Tm values ranging from 14.7 to 20.5 • C. In each sub-series, it could be observed that the best phenanthroline heterocyclic compounds 1 which exhibited an interesting stabilization profile were those substituted by the longest aminoalkylaminomethyl side chains.For example, for each G-quadruplex sequence, the substituted diphenylphenanthro-line ligand 1d bearing (dimethylaminopentyl)aminomethyl side chains exhibited the best strong stabilization profile in comparison with their (dimethylaminobutyl)aminomethyl or (dimethylaminopropyl)aminomethyl or (dimethylaminoethyl)aminomethyl substituted homologues 1a-c; these latter being least stabilizing ligands.The same observations could also be

ticed for derivative 1g
versus 1e-f; 1i versus 1h; and also compound 1k versus 1j.Concerning the position of the substitution of the pyridine gro

Native Electrospray Mass Spectrometry and Circular Dichroism
Native mass spectrometry is the technique of choice to determine the binding affinity and stoichiometry of small molecules with oligonucleotides [54].Here we performed native ESI-MS experiments with the ligands 1c, 1d, 1l and 1m and the G4-forming oligonucleotides Pf1 (d[(G 3 T 3 A) 3 G 3 ]), Pf8 (d[(G 3 T 2 CA) 3 G 3 ]) and the human telomeric sequence 24TTG (Table 5).Two non-G4 forming controls were also used: the duplex-forming sequence DK66 (d[(CG) 2 A 2 T 2 (CG) 2 ]) and a G-rich unfolded single-stranded sequence, 24nonG4 (d[TG 3 ATGCGACA(GA) 2 G 2 ACG 3 A]).All tested structures contain 24 nucleotides.Pf1 and Pf8 are the same Plasmodium telomeric sequences used in the FRET meting experiments.Their CD signature suggests the formation of hybrid conformers (positive band at 290 nm, shouldering at 270 nm; Figure S1) 4TTG (d[T 2 G 3 (T 2 AG 3 ) 3 A]) is a variant of the human telomeric sequence (similar to the 21T sequence used in the FRET melting experiments) whose hybrid structure in native MS conditions is known [55], and was verified here by CD (Figure S1).UV-melting experiments indicate that these three G4forming oligonucleotides form G4s of similar stabilities (T m = 38.2,38.7 and 40.3 • C, respectively).They are fully folded at 22 • C, in our mass spectrometry buffer (folded fraction θ~1.0; Figure S2).Native MS spectra confirms the quantitative formation of G4 conformers binding two K + , with small amounts of 1K + complexes detected in the case of Pf1 (Figure S3).1m does not bind any of the assayed oligonucleotides (including controls), expect for the oligonucleotide Pf8 that it very weakly bound (Figures 3 and S3).This is consistent with the absence of stabilization observed by FRET melting.
1c, 1d, and 1l bind moderately to all three G4-forming oligonucleotides (Figures 3 and S3, Table 3).Affinities for any given sequence are similar across these three ligands, with 1c being somewhat less affine than 1d and 1l.Both 1:1 and 2:1 ligand:DNA complexes are formed, with the exception of 1c that only forms 1:1 complexes with Pf1.The tightest binding occurs with Pf8 (K d1 = 2-3 µM and K d2 = 16-59 µM).Pf1 is bound with lower affinities (K d1 = 8-23 µM and K d2 = 24-37 µM).The amount of bound 24TTG is even lower, yet 2:1 complexes can still be observed, highlighting the presence of two weak binding sites of relatively close affinities (K d1 = 34-44 µM and K d2 = 18-62 µM).Remarkably, the K + adduct distribution is altered upon ligand binding for all these complexes (Figures 3 and S3).Specifically, the distribution shifts toward the 1K + species, hinting at the formation of two-tetrad conformers.It is more evident for Pf1 than Pf8, consistent with the former already forming a small amount of 1K + species in absence of ligand.Binding of a second ligand further shifts the equilibrium toward the 1K + species.The CD spectra shift toward more antiparallel signatures (positive band at 290 nm, negative band at 260 nm) with increasing ligand concentrations (Figure S1).This is consistent with the formation of two-tetrad, intramolecular G4s coordinating potassium that usually adopt an antiparallel topology [55,56].Alternatively, this might indicate intercalation of the ligand as recently observed [57].
Small amounts of 2:1 complexes with 24TTG devoid of potassium adduct are observed.1c, 1d, and 1l may therefore promote the unfolding of 24TTG, although to a limited extent.This is supported by their binding to the G-rich non-G4 24nonG4 oligonucleotide with dissociation constants in the micromolar range (K d1 = 8-11 µM).Complexation of 24nonG4 does not lead to a shift to larger K + stoichiometries, which could have indicated folding into G4 conformers.CD spectra show that the conformation of 24nonG4 is somewhat altered upon ligand binding, but does not support G4 formation either.These ligands are therefore likely to be able to bind to non-G4 conformations of G-rich oligonucleotides.

Conclusions
In this work, we described the design, synthesis, antiprotozoal activities, and the in vitro cytotoxicity toward human cells of a novel series of 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthroline derivatives.These new phenanthrolines were tested for their in vitro antiprotozoal activity toward the CQ-resistant W2 and CQ-sensitive 3D7 P. falciparum strains, the promastigote form of L. donovani, and also toward a T. brucei brucei strain.In addition, the in vitro cytotoxicity of these new nitrogen heterocyclic compounds was assessed on the human HepG2 cell line.Among these novel synthesized nitrogen heterocyclic molecules, a few of them were identified as interesting potential in vitro antiplasmodial leads with IC 50 ranging from 0.03 to 0.73 µM on the W2 and 3D7 strains of P. falciparum.Interestingly, the 2,9-bis [3-(pyrrolidin-1-yl)propyl)aminomethyl]-4,7-diphenyl-1,10-phenanthroline 1l was identified as the most potent and promising antimalarial candidate with a ratio of cytotoxic to antiparasitic activities of 500 against the P. falciparum CQ-resistant strain W2.Four substituted phenanthrolines showed activity against the promastigote forms of L. donovani with IC 50 values ranging from 2.52 to 4.50 µM.Moreover, the antiprotozoal activity spectrum of our new synthesized phenanthrolines using a T. brucei brucei strain revealed IC 50 values ranging from 0.20 to 1.42 µM, which warrant further investigations.The 2,9-bis [2-(pyridin-4-yl)ethyl)aminomethyl]-4,7-diphenyl-1,10phenanthroline 1o was also identified as the most potent trypanosomal candidate with SI of 91 on Trypanosoma brucei brucei strain.Structure-activity relationships of these new synthetic compounds are also discussed, as well as their relative ability to target P. falciparum or Trypanosoma telomeres as a hypothetical mechanism of action and a potential way to escape the efflux resistance developed by parasites toward quinoline drugs.Thus, as the telomeres of the parasites could constitute interesting targets, we also investigated the possibility of targeting Plasmodium telomeres or Trypanosoma chromosomes by stabilizing the Plasmodium or Trypanosoma G-quadruplexes sequences through FRET melting assays with our bioactive phenanthrolines.Concerning the stabilization of the parasitic G-quadruplex, it could be noticed that the best diphenylphenanthrolines 1 which exhibited an interesting stabilization profile were those substituted by the longest aminoalkylaminomethyl side chains.However, we found no correlation between the antiparasitic activity and selectivity of these derivatives and their binding to G-quadruplexes.These derivatives are therefore unlikely to be specifically cytotoxic via G-quadruplex binding.Moreover, it would be now interesting to enlarge the biological evaluation of these new diphenylphenanthrolines derivatives 1 by studying their mechanism of action through further investigations such as the inhibition of beta-hematin formation or of the apicoplast functions.Finally, the new substituted 2,9-bis[(substituted-aminomethyl)]-4,7-phenyl-1,10-phenanthrolines could open the way to new valuable medicinal chemistry scaffolding in the antiprotozoal domain.

Figure 2 .
Figure 2. Stabilization specificity profile of 1d, 1g, and 1l (2 µM) toward various G4 oligonucleotides.The difference in Tm in the presence and absence of 1d, 1g, and 1l, ∆Tm, in • C is plotted for each sequence quadruplexes and one duplex (FdxT) were tested.
a The stoichiometry and composition of the salts were determined by elemental analyses and obtained values were within ±0.4% of the theoretical values.b Crystallization solvent: 2-PrOH-H 2 O. c The yields only included the conversions into the ammonium oxalates.

Table 2 .
In vitro sensitivity of P. falciparum, L. donovani and T. brucei brucei strains to compounds 1a-p and cytotoxicity of these compounds in HepG2 cells.

Table 3 .
Selectivity indexes of compounds 1a-p.SI was defined as the ratio between the CC 50 value on the HepG2 cells and the IC 50 value against the P. falciparum W2 or 3D7 or Trypanosoma brucei brucei strains.b n.d.: not determined. a
a n.b.: no binding detected, <LoQ: binding detected but below limit of quantitation.