Reptile Host Associations of Ixodes scapularis in Florida and Implications for Borrelia spp. Ecology

Host associations of the tick vector for Lyme Borreliosis, Ixodes scapularis, differ across its geographic range. In Florida, the primary competent mammalian host of Lyme disease is not present but instead has other small mammals and herpetofauna that I. scapularis can utilize. We investigated host–tick association for lizards, the abundance of ticks on lizards and the prevalence of Borrelia burgdorferi sensu lato (sl). To determine which lizard species I. scapularis associates with, we examined 11 native lizard species from historical herpetological specimens. We found that (294/5828) of the specimens had attached ticks. The most infested species were Plestiodon skinks (241/1228) and Ophisaurus glass lizards (25/572). These species were then targeted at six field sites across Florida and sampled from June to September 2020, using drift fence arrays, cover boards and fishing. We captured 125 lizards and collected 233 immature I. scapularis. DNA was extracted from ticks and lizard tissue samples, followed by PCR testing for Borrelia spp. Of the captured lizards, 69/125 were infested with immature I. scapularis. We did not detect Borrelia spp. from tick or lizard tissue samples. Overall, we found that lizards are commonly infested with I. scapularis. However, we did not detect Borrelia burgdorferi sl. These findings add to a growing body of evidence that lizards are poor reservoir species.


Introduction
In the US Lyme Borreliosis (LB) is the most commonly reported vector-borne disease with approximately 476,000 cases per year [1]. The primary etiological agent of LB in the US is Borrelia burgdorferi sensu stricto (ss). Borrelia burgdorferi ss is part of a global species complex referred to as Borrelia burgdorferi sensu lato (sl) [2]. Species in the B. burgdorferi sl complex vary in their genetic code, pathogenicity, tick vectors, host species and distribution [3,4]. In the US, 10 species in this complex have been reported and are vectored by Ixodes ticks [4]. The distribution of these bacterial species has important implications on LB disease risk since they are not all pathogenic. In the US, two species in the complex are associated with LB: B. burgdorferi ss and B. mayonii. An additional species, B. bissetiae, is tentatively considered an agent of LB [2,3]. All three of the LB-associated genospecies are vectored by Ixodes scapularis.
The distribution of B. burgdorferi sl species varies across North America. In California, B. burgdorferis ss was more frequently detected in the north and central coast, while B. bissetiae was more common in the southern part of the state [3]. In eastern North America, B. burgdorferi sl species prevalence is higher in the northeast and Midwest compared to the southeast [5][6][7]. Multiple B. burgdorferi sl species have been detected in the northeast and Midwest including B. burgdorferi ss and B. mayonii [2,[6][7][8]. The distribution of B. burgdorferi sl species in the southeastern US, however, has not been well established since wildlife hosts and ticks have not been thoroughly assessed [9]. Understanding the tick and wildlife host associations can help provide insight into why there is a low prevalence of B. burgdorferi sl in the southeastern US. Multiple hypotheses have been proposed as to why the prevalence of B. burgdorferi sl species in hosts and ticks is concentrated in the Midwest and northeast but not in the southeast, even though I. scapularis is present in all regions. One hypothesis is that I. scapularis questing height can influence host associations. In the southeast, I. scapularis quest in the leaflitter, while in the northeast, they climb up vegetation, which could influence host associations [10]. An alternative hypothesis is that competent host availability may differ between regions. In the northeast, small mammals are the primary hosts of immature I. scapularis [11]. Peromyscus leucopus (white-footed mouse) is an extremely competent host of I. scapularis immatures and reservoir for B. burgdorferi ss [12]; however, P. leucopus is not present in the southern range of I. scapularis' distribution. Other rodent hosts are present in the southeastern US such as P. gossypinus, which can maintain long-term B. burgdorferi ss and B. bissetiae infections (>47 months) [13]; however, P. gossypinus are more commonly infested by I. minor and I. affinis, which rarely feed on humans compared to I. scapularis [13]. In the European B. burgdorferi sl complex system, different genotypes are associated with specific vertebrate taxa: mammals, birds and reptiles. However, the host association of different B. burgdorferi sl species in North America is not well established [4].
In the southeastern US, many alternative host species are available, on which I. scapularis can feed, including a diversity of lizard species. Ixodes scapularis immatures have been reported to feed on 14 reptile species in the US, and many of them reside in the southeast [14][15][16]. It has not been well studied which lizard species act as hosts for I. scapularis, since few studies examining wildlife for ticks incorporate lizards. In the few studies examining lizard hosts, the focus was placed on tick presence and infestation intensity; Borrelia infections have rarely been surveyed [9,16,17]. From these studies, the most commonly infested lizard species were Plestiodon skinks and Ophisaurus glass lizards [3,8,13,14]. These lizards are commonly found in the leaf litter, which is consistent with tick questing behavior in the southeast [6,16]. Even though multiple species of lizards can be infested with I. scapularis, they are the least studied host in Borrelia epidemiology and other tick-borne pathogens [9,11,[18][19][20]. In the European B. burgdorferi sl complex, lizards are specifically the host of B. lusitaniae, a nonpathogenic species [3]. Whether or not lizards in the US are associated with specific B. burgdorferi sl species is unclear.
Studies addressing Borrelia infections in lizards have had varying implications for the epidemiology of Borrelia transmission cycles. In California, the western fence lizard (Sceloporus occidentalis) plays a crucial role in the ecology of Borrelia burgdorferi ss. The blood of the western fence lizard contains a thermolabile, borreliacidal factor that kills Borrelia spirochetes [21]. Borreliacidals have not been identified in southeastern lizards, and there are reports of lizards infected with Borrelia [22,23]. Experimental infections using infected feeding ticks demonstrated that Plestiodon inexpectatus (southeastern five lined skink) and Anolis carolinensis (green anole) can acquire and maintain Borrelia burgdorferi ss infections [23]. Wild-caught lizards from South Carolina and north Florida have been found to be infected with species from the B. burgdorferi sl complex with a 50% prevalence from blood samples [22]. Another study of Plestiodon spp. collected across the southeast suggested that the skinks had zooprophylactic effect on B. burgdorferi ss infection [24]. In this study, I. scapularis nymphs infected with B. burgdorferi ss were allowed to feed on skinks. After feeding, the number of infected nymphs decreased from 71.4% to 7.4% [24]. The exact role of lizard hosts in the transmission of Borrelia is still ill-defined and likely complex.
Florida is one of the most herpetologically diverse states [25], and Ixodes scapularis has been reported or has established populations in all 67 Florida counties. Cases of autochthonous LB have been reported in 45/67 Florida counties [26][27][28]. Studies that have examined reptile hosts of I. scapularis in Florida have only sampled within the northern part of the state [6,[22][23][24], yet Florida constitutes the most southern range of the I. scapularis distribution in the US. How lizards influence the epidemiology of Borrelia in the most southern part of I. scapularis range has not been thoroughly investigated. Given Florida's diverse lizard community, distribution of I. scapularis and LB cases, further investigations into the role of lizards in the epidemiology of Borrelia are warranted.
To determine if lizards play a role in Borrelia epidemiology in Florida, we examined several different components of host competency. A competent reservoir host species must fulfill three criteria. First, a host and vector species must have interactions with one another. Are ticks feeding on lizards? Secondly, the host species must be able to maintain a pathogen infection. Are lizards infected with Borrelia? Lastly, the host must be able to infect a vector species. Can feeding ticks obtain the pathogen from feeding on lizards?
The objective of our investigation was to determine the role of native lizards in Florida for the first two criteria of host competency. We addressed the following questions. (1) Are I. scapularis ticks feeding on lizards in Florida? To address whether ticks and lizards interact, we examined museum specimens from historical and current collections for I. scapularis infestations. After determining tick-host associations of lizards we applied this knowledge to field sampling of lizards. We collected additional data on tick infestation from fieldcaptured lizards. We then focused on the second aspect of host competency: (2) Are lizards infected with Borrelia or other tick-borne bacteria? Using field-collected ticks and tissue samples from captured lizards, we tested for the presence of Borrelia infections and other tick-borne bacterial pathogens.

Museum Specimen Survey
We examined 11 species of native lizards, specimens of which were collected from all 67 counties. From the herpetological collection, we found that 5.0% (294/5828) of the specimens of all 11 species we examined were infested with ticks from 43 of the 67 Florida counties (Table 1). We found two tick species on the lizard specimens: I. scapularis and Amblyomma americanum. Table 1. Lizard species surveyed for ticks and pathogens. The number in parentheses next to the species names represents the number of lizard tissue samples from the museum tissue repository tested for bacterial pathogens. Additionally, presented are the average number (±SE) of larvae and nymphs I. scapularis removed from individual herpetological specimens and the abundance (no. of lizards infested with ticks/no. of lizards sampled) and percentage of lizards infested with ticks.

Species
Avg Sceloporus undulatus was the only lizard to be infested with both tick species. Of the S. undulatus examined, 11 were infested with I. scapularis and 9 with A. americanum. None of the S. undulatus specimens were coinfested with both tick species. All other ticks identified on museum specimens were identified as immature I. scapularis. Ixodes scapularis were found on lizards which were collected as early as 1927 on P. laticeps specimens from Alachua County.
From the museum specimens, we found that 8/11 native lizard species were infested with ticks. Plestiodon was the most commonly infested lizard genus with ticks. Plestiodon fasciatus, P. inexpectatus, and P. laticeps had the greatest number of attached ticks per individual on average (Table 1). Plestiodon egregius was the only Plestiodon spp. to have Pathogens 2021, 10, 999 4 of 12 only one specimen infested with ticks. Ophisaurus glass lizards also had tick infestations, although few individuals were infested compared to Plestiodon spp.

Field Collected Lizards and Ticks
From our six field sites, we live captured a total of 125 lizards ( Figure 1). We captured 123 individuals of Plestiodon spp. and two O. ventralis ( Figure 1). The majority of captured lizards came from the two most northern sites, which mostly consisted of P. fasciatus and P. laticeps.
From the museum specimens, we found that 8/11 native lizard species were infested with ticks. Plestiodon was the most commonly infested lizard genus with ticks. Plestiodon fasciatus, P. inexpectatus, and P. laticeps had the greatest number of attached ticks per individual on average (Table 1). Plestiodon egregius was the only Plestiodon spp. to have only one specimen infested with ticks. Ophisaurus glass lizards also had tick infestations, although few individuals were infested compared to Plestiodon spp.

Field Collected Lizards and Ticks
From our six field sites, we live captured a total of 125 lizards (Figure 1). We captured 123 individuals of Plestiodon spp. and two O. ventralis (Figure 1). The majority of captured lizards came from the two most northern sites, which mostly consisted of P. fasciatus and P. laticeps. Plestiodon inexpectatus was the most widely distributed lizard species; we collected them from five of six field sites. Ophisaurus ventralis were only captured from one central field site and not infested with ticks. We captured fewer lizards at the southern sites compared to the northern sites.
Of the field collected lizards, 69/125 (55.2%) were infested with I. scapularis immatures (Table 2). In total we collected 233 (29 larvae, 204 nymphs) I. scapularis from the lizards. No other tick species were found on the field-collected lizards. We collected I. scapularis at five out of six field sites ( Table 2). The majority of the ticks collected were from lizards in the northern field sites ( Table 2). The central and southern field sites had lizards with fewer attached ticks. Plestiodon inexpectatus was the most widely distributed lizard species; we collected them from five of six field sites. Ophisaurus ventralis were only captured from one central field site and not infested with ticks. We captured fewer lizards at the southern sites compared to the northern sites.
Of the field collected lizards, 69/125 (55.2%) were infested with I. scapularis immatures ( Table 2). In total we collected 233 (29 larvae, 204 nymphs) I. scapularis from the lizards. No other tick species were found on the field-collected lizards. We collected I. scapularis at five out of six field sites ( Table 2). The majority of the ticks collected were from lizards in the northern field sites ( Table 2). The central and southern field sites had lizards with fewer attached ticks. Three Plestiodon species were infested with ticks: P. fasciatus, P. inexpectatus, and P. laticeps, which was consistent with the museum specimen findings ( Figure 1, Table 2). On average, Plestiodon spp. were infested with 3.4 ticks per individual (Table 3). Of the Plestiodon spp., we captured male, female, and juvenile individuals (Table 3). We found that P. laticeps had the greatest abundance of individuals infested with ticks compared to the other Plestiodon spp. (Table 2). All the P. laticeps individuals caught in our study were adults and all individuals were infested with ticks. Plestiodon fasciatus individuals were also commonly infested with ticks (66.7%). Plestiodon inexpectatus had the lowest average number of attached ticks and the lowest abundance of individuals infested with ticks (Table 3). We examined the abundance (no. of lizards infested with ticks/no. of lizards sampled) of tick-infested lizards across Florida from museum and field specimens (

Pathogen Screening
We screened 103 tissue samples from the same 11 Florida native species from the Genetic Repository of the Florida Museum on Natural History (Table 1). No samples tested positive for any bacterial pathogens (Ehrlichia spp., Anaplasma spp., Borrelia spp., Rickettsia spp.).
We also screened field-collected tick and lizard tissue samples for tick-borne bacterial pathogens. We did not detect any Borrelia spp. in the tick or lizard tissue samples. Samples were also negative for the presence of Anaplasma spp. and Ehrlichia spp. Field-collected ticks tested positive for Rickettsia spp. for both the gltA and ompA gene. For the gltA gene primer set, we found that 14/69 tick pools (pooled by individual lizard) tested positive and matched closely with a Rickettsia spp. (GenBank Assession No. CP060138). With the ompA gene primers, we detected Rickettsia spp. in ticks pooled by individual (16/68). This sequence was similar to that of an I. scapularis rickettsial endosymbiont (GenBank Assession No. EF689735).

Discussion
The goal of our investigation was to address two aspects of host competency. (1) Do ticks and lizards interact? (2) Are lizards infected with Borrelia burgdorferi sl?
To investigate if lizards and ticks interacted with each other in Florida, we surveyed historical herpetological specimens and field-captured lizards for tick infestations. We found that 5.0% of the herpetological specimens and 55.2% of field-collected lizards were infested with ticks. Plestiodon spp. and Ophisaurus spp. were the most common hosts of I. scapularis immatures throughout Florida. Our results are consistent with other hostassociation studies in the southeastern US for lizards [16,20,24]. One other study surveyed for ticks on museum specimens collected in North Carolina [19]. In that study, the authors found that 8.7% of the herpetological specimens were infested with I. scapularis immatures. While we found a similar abundance of infested ticks for P. inexpectatus as found in North Carolina (13.8%), P. laticeps (7.4%) and P. fasciatus (3.0%) in North Carolina had a lower abundance of infested ticks than in Florida specimens.
Anolis carolinensis is a common lizard species throughout Florida and has been shown to be a competent reservoir host of B. burgdorferi ss in the laboratory [23]. Of the 1241 museum specimens collected from Florida, none were infested with ticks. In North Carolina, 3.2% (6/187) of the A. carolinensis examined were infested with larval ticks [23]. This apparent difference may have an ecological explanation driven by an invasive anole in Florida. Anolis sagrei, the brown anole, is a common invasive lizard species in Florida. In the presence of A. sagrei, A. carolinensis increases their arborealism [29]. These behavioral changes may reduce the likelihood of A. carolinensis interacting with I. scapularis, which quests in the leaf litter. Thus, it appears that A. carolinensis is not a likely competent host for Borrelia spp. given its lack of interaction with I. scapularis, in the presence of A. sagrei.
It is important to note that tick-host association data from museum specimens must be interpreted with caution [19,30]. We found lower tick abundance in the museum specimens compared to the field specimens. Museum specimens are preserved after an animal has died. The time between death and preservation may allow ticks time to vacate their host and not be included with the preserved specimen, altering estimates of tick abundance and the intensity of individual infestation. Sampling bias, including the location, time, and sex of specimens, may also influence the occurrence of ticks on the collected specimen. In our museum specimens, Florida counties were not all equally represented in sampling efforts. We are likely missing data from certain populations throughout the state. Even with these biases, we were able to detect the occurrence of ticks infesting lizards in 43/67 of the Florida counties.
Using the museum specimens, we were able to survey a wide diversity of lizards to determine which species were associated with Ixodid ticks. Museum specimens provide a convenient way to identify host species but are likely not as useful for comparing infestation Pathogens 2021, 10, 999 7 of 12 rates or determining the absence of a tick species. Nonetheless, museum collections help clarify tick-host associations and can assist in validating those associations in the field. Studying wildlife infested with ticks requires various permitting processes and animal care requirements. By predetermining targeted host species with museum data, investigators can provide permitting agencies preliminary data on why a particular species needs to be surveyed.
To complement our data from museum collections, we field-collected Plestiodon spp. to determine the magnitude of the interaction with I. scapularis in Florida. We found that 56.1% of the Plestiodon spp. we captured were infested with larval and nymphal I. scapularis. Most of our infested lizards came from two northern sites, where we collected mostly P. fasciatus and P. laticeps. The distribution of P. fasciatus and P. laticeps are both restricted to northern Florida and the Panhandle [23]. We caught P. inexpectatus across all our field sites as expected given its broad distribution across Florida. Although only a few individuals were captured at each site (n = 1-16), we found lizards with ticks at central and southern field sites. Plestiodon inexpectatus was found to be a competent host for Borrelia bacteria in a laboratory setting; yet in the field, they had the least number of ticks infesting them compared to the other Plestiodon spp. [23]. All three Plestiodon spp. are sympatric in forested habitats throughout the southeastern US [31]. Temperature influences how these three species partition themselves within their microhabitat [31]. Plestiodon inexpectatus can utilize warmer open habitats compared to the other Plestiodon spp. [31]. The use of warmer habitats may reduce possible tick interactions of P. inexpectatus since ticks are sensitive to desiccation [32].
Because lizards are common hosts of I. scapularis immature life stages in Florida, they may be important hosts for maintaining tick populations compared to northeastern or Midwest US [6]. I. scapularis in the southeastern US quest in leaf litter to avoid desiccation, as opposed to northern populations that climb up vegetation to quest [33,34]. Plestiodon spp. are common lizards that forage for arthropods in the leaf litter, which increases their likelihood of encountering I. scapularis [34,35]. Indeed, in California, lizard hosts are important for maintaining I. pacificus populations [36]. When lizards were removed from experimental plots, I. pacificus populations decreased [32]. However, I. pacificus feed preferentially on lizard hosts, which could have influenced the tight association of host and tick [36,37]. Previous studies of I. scapularis found that larvae preferred mice, but nymphal ticks did not display a preference [37,38]. Whether lizards are crucial for maintaining I. scapularis populations in the southeast has yet to be investigated.
To address the second aspect of host competency, we screened lizard tissue samples for Borrelia burgdorferi sl to determine if Borrelia spp. were disseminated in host tissue. We did not detect Borrelia burgdorferi sl in any lizard tissue samples from our field or museum collection. Nor did we find Borrelia spp. in any of the ticks that we collected from lizards in the field. Although previous studies surveying ticks in the southeastern US found Borrelia burgdorferi sl at a low prevalence compared to the northeastern US [5,6,39], few studies have tested lizards and their attached ticks from field collected samples. While we can only speculate if our negative results were due to a lack of host competency, some lizard species have been previously hypothesized to have decreased competency due to a borreliacidal factor that decreases transmission of Borrelia spp. The borreliacidal factor has been shown to kill spirochetes in blood and sera samples of Sceloporus occientalis in western North America [21]. Xenodiagnositcs on Plestiodon spp. and S. undulates have been conducted and found that the number of infected ticks decreased after feeding on lizards [24,40,41]; however, the mechanism underlying decreased infection rates has not been identified. Xenodiagnostic studies apply infected immature ticks to lizards and then test the fed larvae for Borrelia burgdorferi ss infections. It is currently unknown if the Plestiodon species investigated in this study have the ability to curtail infections.
Tail snips are a commonly collected tissue type in herpetological studies. Tail snips are a simple tissue to sample since lizards naturally drop their tails to escape from predators. Studies have used lizard tail snips previously for host competency investigations and found Borrelia burgdorferi sl infections in lizards from Florida, Maryland, and South Carolina [22][23][24]42]. However, Borrelia bacteria can disseminate throughout the host body to multiple tissues, and the collection of tissue from additional internal organs could provide more insight on lizard infection status [23]. A laboratory study found that kidney and liver tissues of P. inexpectatus can be infected with Borrelia burgdorferi ss [23]. Our study used a combination of tail, liver, and skeletal muscle for our Borrelia assays so that we could detect Borrelia if it was disseminated in different parts of the lizard. In addition, our sample size was adequate; we sampled 228 lizards, yet a sample size of only 73 lizards was estimated as necessary to determine an apparent prevalence of 0.01 with a 95% CI. Because tick and lizard tissue samples tested negative, our results provide further evidence that lizards are unlikely to be important reservoir species for Borrelia in Florida. Future investigations should focus on other vertebrate hosts as potential competent hosts of Borrelia.
Overall, we found that lizards are important hosts for I. scapularis but did not detect Borrelia burgdorferi sl in the tissues sampled. Tick-host association is an important factor in enzootic transmission of Borrelia bacteria. Previous investigations of Lyme disease transmission have shown that nymphal infection density (DIN) is a crucial indicator of disease risk [43,44]. Infected nymphs are important for infecting naive larvae and host species because B. burgdorferi sl species can be horizontally transmitted during cofeeding on a host [45]. In our study, we found both immature stages on lizards, but no Borrelia burgdorferi sl infections. Since both larvae and nymphs are feeding on potentially poor reservoir host (lizards), the DIN is low, which may reduce the risk of disease transmission [44]. These findings provide further evidence to support that the availability of competent reservoir hosts across the latitudinal gradient plays an important role in Lyme disease risk [6].
In addition to screening for Borrelia burgdorferi sl, we also surveyed our tick and lizard tissue samples for additional tick-borne bacterial pathogens. Ixodes scapularis is a known vector of other bacterial pathogens including Anaplasma phagocytophilum and Ehrlichia muris euclairensis [46]. From our tick samples, we only detected the presence of Rickettsia spp. which are likely endosymbionts based on GenBank matches to our sequences. Our lizard tissue samples all tested negative for bacterial pathogens, providing evidence that lizard species are unlikely reservoir species for other bacterial pathogens.

Museum Samples
Native lizard specimens from the Florida Museum of Natural History Herpetology Collection were examined for the presence and intensity of tick infestations. Eleven species of lizards were selected for examination based on previous host records and presence in Florida [47]. Lizard specimens were collected between 1902-2018 and collected from all 67 Florida counties. Lizards were examined under a dissecting scope to visualize any larval or nymphal ticks. Ticks were then carefully removed from reptiles using fine-tipped forceps and placed in vials containing 70% ethanol. Ticks were identified to species using taxonomic keys [48][49][50]. Based on the results of the museum survey, we targeted specific species of lizards in the field.

Field Sampling of Lizard and Ticks
The presence of Ixodes scapularis has been reported in all Florida counties [26]. To incorporate the full distribution of I. scapularis we live sampled lizards from two field sites each in northern, central and southern Florida (N = 6) ( Figure 1). Our 2 northern sites were near locations that previously detected Borrelia burgdorferi sl from lizards [22,24]. Based on the survey of museum specimens, we targeted Plestiodon skinks in our field study. Ticks were collected from lizards at the field sites from June to September 2020, which coincides with the Plestiodon spp. breeding season [25,51]. Each field site was sampled for a two-week period. Lizards at each site were captured using drift fences, cover boards, hand capture, or lizard fishing [52]. Collection methods were approved by University of Florida Institutional Animal Care and Use Committee (#201910934) and the Florida Fish and Wildlife Commission (FWC) (LSSC-20-00001). Captured lizards were visually examined for ticks using a magnifying glass. If ticks were present, they were removed with fine tip forceps and placed into vials with 100% molecular-grade ethanol. A 1 cm tail snip was taken from each lizard captured. Tails snips were placed in vials with 100% molecular grade ethanol. Lizards were subsequently released at the point of capture.

PCR Testing and Sequencing
To determine if lizards are potential reservoirs for bacterial pathogens, including Borrelia spp., we obtained 103 tissue samples from 11 native lizard species from the Genetic Repository of the Florida Museum of Natural History (Table 1). Tissue samples were collected between 2007-2018. Tissue sample types included liver (10), muscle (86), and tail tip (7). We also collected tail snips from 125 field-captured lizards. Ticks collected from captured lizards were pooled by individual lizard for DNA extraction. Tick specimens from the museum collection were not screened for pathogens since herpetological specimens are fixed with formalin which inhibits PCR.
All lizard tissue (museum and live capture) and field-collected tick samples were extracted for DNA using the Qiagen Gentra Purgene kit using the manufacture protocol (Qiagen, Valencia, CA, USA). Eluted DNA was stored at −20°C until PCR was conducted. We ran PCR's for 4 partial gene sequences of Borrelia: 16S, flab, IGS, and OspA [53][54][55]. We additionally tested for other tick-borne bacterial pathogens: Anaplasma spp., Ehrlichia spp. and Rickettsia spp. For Anaplasma and Ehrlichia we screened for the groESL gene [55]. For Rickettsia we screened with gltA which broadly screens for multiple species of Rickettsia. We also screened for Spotted Fever Group Rickettsia using the ompA gene [56]. The positive control for Borrelia used in our PCR was a construct of B. burgdorferi ss B-31 (ATCC ® 35210™). For the Anaplasma positive control, a supernatant from dog cells infected with A. phagocytophilum was used. The Rickettsia-positive control was DNA from a Rickettsia sp. endosymbiont extracted from an I. scapularis. A negative control was run for each PCR assay with PCR-grade water. All PCR products were run on a 1.5% gel with RedView Stain (Genecopoeia, Rockville, MD, USA) and visualized on UVP gel documentation system (Analytik-Jena, Beverly, MA, USA). Positive samples were cleaned with SAP/Exonuclease and sent to Eurofins (Sanger Sequencing, Louisville, KY, USA) for sequencing to determine pathogen species. Sequences were aligned using Geneious (v 11.1) and compared to sequences in GenBank using NCBI BLAST.

Conclusions
In our investigation, we addressed two components of host competency: (1) Are I. scapularis ticks feeding on lizards in Florida? (2) Are lizards infected with Borrelia burgdorferi sl? Overall, we found that multiple lizard species in Florida are common hosts for I. scapularis immatures. Based on our study, Plestiodon skinks are common hosts of I. scapularis as evidenced by museum specimens and field-collected data from Florida. With multiple Plestiodon species distributed across the entire state, I. scapularis have hosts that can maintain immature life stages. We did not detect Borrelia burgdorferi sl or other tick-borne bacterial pathogens from ticks or lizard tissue samples. Our study provides further evidence that Plestiodon spp. are unlikely reservoir host species for tick-borne bacterial pathogens in Florida.