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Article

A Cross-Sectional Study of SARS-CoV-2 Seroprevalence between Fall 2020 and February 2021 in Allegheny County, Western Pennsylvania, USA

1
Division of Infectious Diseases, Department of Pediatrics, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
2
Center for Vaccine Research, University of Pittsburgh, Pittsburgh, PA 15261, USA
3
Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, PA 15261, USA
4
Department of Pathology, School of Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA
5
Department of Immunology, University of Pittsburgh, Pittsburgh, PA 15261, USA
*
Author to whom correspondence should be addressed.
Academic Editors: Philipp A. Ilinykh and Kai Huang
Pathogens 2021, 10(6), 710; https://doi.org/10.3390/pathogens10060710
Received: 10 May 2021 / Revised: 2 June 2021 / Accepted: 4 June 2021 / Published: 6 June 2021
(This article belongs to the Special Issue Characterization of Antibody Responses to Virus Infections in Humans)
Seroprevalence studies are important for understanding the dynamics of local virus transmission and evaluating community immunity. To assess the seroprevalence for SARS-CoV-2 in Allegheny County, an urban/suburban county in Western PA, 393 human blood samples collected in Fall 2020 and February 2021 were examined for spike protein receptor-binding domain (RBD) and nucleocapsid protein (N) antibodies. All RBD-positive samples were evaluated for virus-specific neutralization activity. Our results showed a seroprevalence of 5.5% by RBD ELISA, 4.5% by N ELISA, and 2.5% for both in Fall 2020, which increased to 24.7% by RBD ELISA, 14.9% by N ELISA, and 12.9% for both in February 2021. Neutralization titer was significantly correlated with RBD titer but not with N titer. Using these two assays, we were able to distinguish infected from vaccinated individuals. In the February cohort, higher median income and white race were associated with serological findings consistent with vaccination. This study demonstrates a 4.5-fold increase in SARS-CoV-2 seroprevalence from Fall 2020 to February 2021 in Allegheny County, PA, due to increased incidence of both natural disease and vaccination. Future seroprevalence studies will need to include the effect of vaccination on assay results and incorporate non-vaccine antigens in serological assessments. View Full-Text
Keywords: SARS-CoV-2; seroprevalence; ELISA; neutralization assay SARS-CoV-2; seroprevalence; ELISA; neutralization assay
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MDPI and ACS Style

Xu, L.; Doyle, J.; Barbeau, D.J.; Le Sage, V.; Wells, A.; Duprex, W.P.; Shurin, M.R.; Wheeler, S.E.; McElroy, A.K. A Cross-Sectional Study of SARS-CoV-2 Seroprevalence between Fall 2020 and February 2021 in Allegheny County, Western Pennsylvania, USA. Pathogens 2021, 10, 710. https://doi.org/10.3390/pathogens10060710

AMA Style

Xu L, Doyle J, Barbeau DJ, Le Sage V, Wells A, Duprex WP, Shurin MR, Wheeler SE, McElroy AK. A Cross-Sectional Study of SARS-CoV-2 Seroprevalence between Fall 2020 and February 2021 in Allegheny County, Western Pennsylvania, USA. Pathogens. 2021; 10(6):710. https://doi.org/10.3390/pathogens10060710

Chicago/Turabian Style

Xu, Lingqing, Joshua Doyle, Dominique J. Barbeau, Valerie Le Sage, Alan Wells, W. P. Duprex, Michael R. Shurin, Sarah E. Wheeler, and Anita K. McElroy. 2021. "A Cross-Sectional Study of SARS-CoV-2 Seroprevalence between Fall 2020 and February 2021 in Allegheny County, Western Pennsylvania, USA" Pathogens 10, no. 6: 710. https://doi.org/10.3390/pathogens10060710

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