Strong Inhibitory Activity and Action Modes of Synthetic Maslinic Acid Derivative on Highly Pathogenic Coronaviruses: COVID-19 Drug Candidate

In late December 2019, a novel coronavirus, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), escaped the animal–human interface and emerged as an ongoing global pandemic with severe flu-like illness, commonly known as coronavirus disease 2019 (COVID-19). In this study, a molecular docking study was carried out for seventeen (17) structural analogues prepared from natural maslinic and oleanolic acids, screened against SARS-CoV-2 main protease. Furthermore, we experimentally validated the virtual data by measuring the half-maximal cytotoxic and inhibitory concentrations of each compound. Interestingly, the chlorinated isoxazole linked maslinic acid (compound 17) showed promising antiviral activity at micromolar non-toxic concentrations. Thoughtfully, we showed that compound 17 mainly impairs the viral replication of SARS-CoV-2. Furthermore, a very promising SAR study for the examined compounds was concluded, which could be used by medicinal chemists in the near future for the design and synthesis of potential anti-SARS-CoV-2 candidates. Our results could be very promising for performing further additional in vitro and in vivo studies on the tested compound (17) before further licensing for COVID-19 treatment.


Introduction
Since its emergence, coronavirus disease 2019 (COVID- 19) has triggered a scary and contagious global pandemic of severe pneumonia-like disease, which causes respiratory

Introduction
Since its emergence, coronavirus disease 2019 (COVID-19) has triggered a scary and contagious global pandemic of severe pneumonia-like disease, which causes respiratory illness and acute respiratory distress syndrome in human. Up to now, the race is still on to discover specific and efficient anti-viral drugs against SARS-CoV-2 to control COVID-19 to minimize its awful spread and transmission [1]. To this point, researchers have tried to make use of the existing anti-viral drugs that might combat the infection based on in silico predictions, or based on previous knowledge of other coronaviruses such as SARS-CoV and MERS-CoV. Therefore, a lot of FDA approved drugs have been tested or repositioned to act as anti-SARS-CoV-2 [2,3]. In parallel, several trials to virtually recommend or practically test the anti-SARS-CoV-2 activity of synthetic compounds, natural extracts and immunomodulatory agents have been attempted [4][5][6][7].
Triterpenes are a class of natural molecules made up of 30 carbon atoms. Biosynthetically, the 200 different skeletons known to date and isolated from natural sources come from the cyclization of (3S)-2,3-epoxy-2,3-dihydrosqualene and sometimes of squalene itself. These molecules are in most cases hydroxylated at C-3 position due to the opening of the epoxide during cyclization [8]. In the plant kingdom, terpenoids are secondary metabolites, whose ecological role has been proven, especially in the process of communications and defense. Triterpenes, of which more than 30,000 structures were previously isolated and characterized, is derived from natural sources or enzymatic reactions. Additionally they comprise 47 sub-classes with enormous chemical diversity [8][9][10][11].
Triterpenes are among the natural substances with promising biological, therapeutic and industrial value. In recent years, due to the broad spectrum of their biological potentials, triterpenes have been the subject of an intensive number of research works, mainly focused on their biopharmaceutical development. Indeed, triterpenes and their structural analogues display very varied biological effects such as hemolytic, hypocholesterolimic immunostimulant, anti-inflammatory, antimicrobial, antiparasitic, cytotoxic, and anti-ulcer [12][13][14][15][16][17][18]. Moreover, triterpenes are known for their many important antiviral activities such as anti-hepatitis B virus activity, anti-HIV1 and 2, AIDS, and (to remove) anti-hepatitis C virus activity, and anti-Herpes simplex virus activity ( Figure 1) [11,19]. These compounds and their antiviral activity have seriously attracted the attention of organic chemists, who have thought of chemically modifying them in different ways in order to improve their activities. Some of these analogues are employed as medicinal drugs to treat liver-related diseases [20].
Oleanolic acid (OA, 1) and maslinic acid (MA, 2) are triterpenoids isolated from several plants; in particular, from Olea europaea L. that possesses a variety of interesting biological activities. OA displays several biological potentials including antimicrobial, anticancer, and anti-inflammatory effects [19,21,22]. It has been found to inhibit the entry of several viruses such as HIV1 replication in acutely infected H9 lymphocyte cells [21][22][23] In recent decades, OA has undergone several chemical modifications in order to synthe- These compounds and their antiviral activity have seriously attracted the attention of organic chemists, who have thought of chemically modifying them in different ways in order to improve their activities. Some of these analogues are employed as medicinal drugs to treat liver-related diseases [20].
Oleanolic acid (OA, 1) and maslinic acid (MA, 2) are triterpenoids isolated from several plants; in particular, from Olea europaea L. that possesses a variety of interesting biological activities. OA displays several biological potentials including antimicrobial, anticancer, and anti-inflammatory effects [19,21,22]. It has been found to inhibit the entry of several viruses such as HIV1 replication in acutely infected H9 lymphocyte cells [21][22][23]. In recent decades, OA has undergone several chemical modifications in order to synthesize new structural analogues with promising biological activities. These chemical modifications mainly concerned the C-3 alcohol and C-17 carboxylic acid functions as well as the C12-C13 unsaturation. Various OA congeners have shown antibacterial, anti-acetylcholinesterase, anticancer, anti-inflammatory, and anti-influenza virus activities and were found to be more active than OA [11,15,17,18,21,[23][24][25][26][27].
Maslinic acid (MA) has shown a broad spectrum of biological activity. Indeed, it has been found to be inhibit glycogen phosphorylases [28] and protein tyrosine phosphatase 1B [29]. It also exhibited anti-inflammatory [20], antitumor [30], and anti-HIV-1 activities [31]. Several other interesting biological activities were also reported [15,17,18]. This triterpene acid has attracted the attention of several chemists, who have taken advantage of its various chemical functions to synthesize new series of structural analogues in order to improve some of their activities and also to understand the structure-activity relationship; we cite the example of esters, 3,5-isoxazoles and 1,5-and 1,4-disubstituted triazoles [15,17].
Maslinic acid (MA) has shown a broad spectrum of biological activity. Indeed, it has been found to be inhibit glycogen phosphorylases [28] and protein tyrosine phosphatase 1B [29]. It also exhibited anti-inflammatory [20], antitumor [30], and anti-HIV-1 activities [31]. Several other interesting biological activities were also reported [15,17,18]. This triterpene acid has attracted the attention of several chemists, who have taken advantage of its various chemical functions to synthesize new series of structural analogues in order to improve some of their activities and also to understand the structure-activity relationship; we cite the example of esters, 3,5-isoxazoles and 1,5-and 1,4-disubstituted triazoles [15,17].
Maslinic acid (MA) has shown a broad spectrum of biological activity. Indeed, it has been found to be inhibit glycogen phosphorylases [28] and protein tyrosine phosphatase 1B [29]. It also exhibited anti-inflammatory [20], antitumor [30], and anti-HIV-1 activities [31]. Several other interesting biological activities were also reported [15,17,18]. This triterpene acid has attracted the attention of several chemists, who have taken advantage of its various chemical functions to synthesize new series of structural analogues in order to improve some of their activities and also to understand the structure-activity relationship; we cite the example of esters, 3,5-isoxazoles and 1,5-and 1,4-disubstituted triazoles [15,17].
Compounds bearing isoxazole moiety are of high importance due to their broad spectrum of biological properties including antiviral [32], antidiabetic [33], neuroprotective [34], anticancer and anti-inflammatory activities ( Figure 2) [17,35]. 1,2,3-Triazoles, constituting another important class of heterocycles, widely used in medicinal chemistry, act as pharmacophores and linkers between two or more substances of interest in molecular hybridization approaches [36]. Several of these triazoles have a wide spectrum of biological effects such as anti-HIV [37], antiviral [38], cytotoxic, antiinflammatory [16,18], and antibacterial [39] activities ( Figure 3).  In our previous works, Chouaïb et al. have described the isolation of OA and MA from pomace olive (Olea europaea L.) which have been used as a starting material to prepare new series of congeners constituting a library of novel pentacyclic triterpenoid derivatives bearing esters, 3,5-disubstituted isoxazoles, 1,5-and 1,4-disubstituted triazoles and were synthesized as antimicrobial, anti-inflammatory and anticancer agents [15][16][17][18]. In order to broaden the range of our biological tests on these derivatives taking into account the antiviral effect of oleanolic and maslinic acids as well as of isoxazoles and triazoles, molecular docking studies of OA, MA and most previously synthesized derivatives has been carried out targeting SARS-CoV-2 main protease which allowed one to select seventeen compounds to be evaluated for their antiviral activity against hCoV-19/Egypt/NRC-03/2020 (Accession Number on GSAID: EPI_ISL_430820) "NRC-03-nhCoV" virus [40].
Molecular docking is an important method of computational studies which are very promising tools in the new process of drug discovery and development as well [39]. Concerning the aforementioned crucial rule of main protease (M pro ) for SARS-CoV-2 maturation, and in continuation to our previous work targeting SARS-CoV-2 [2,3,[41][42][43][44], we decided to target SARS-CoV-2 M pro using molecular docking studies and we further validated the antiviral activity of the 17 compounds representing OA, MA and their derivatives. Moreover, we further evaluated the impact of promising anti-SARS-CoV-2 compounds on different stages of the viral replication cycle.

Results and Discussion
Respiratory viruses represent a major public health concern, as they are highly mutated, resulting in the emergence of new strains with high pathogenicity. Currently, the whole world is suffering from a new evolving severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which originally spread from the Hubei region, concisely in Wuhan City in early December 2019, and subsequently spread globally. By the end of January, and due to the increasing rate of SARS-CoV-2-infected confirmed cases associated with relatively high mortality rate, the WHO raised awareness and declared an emergency, claiming a potentially pandemic lethal infectious disease named COVID-19.
Initially, SARS-CoV-2 was considered as a zoonotic disease, based on a high number of infected people exposed to a wet animal market in the Wuhan Chinese city; however, transmission from person to person was confirmed, making the situation even more harsh. This condition resulted in many put quarantines in place, cancelling flights and suspending travel, with the consequence of huge economic burden.
The perceived threat of this pandemic has led many researchers and academic institutions all over the world to dissect COVID-19 from different perspectives, including but not limited to the route of virus transmission, diagnosis, virus inhibition treatments, and vaccine preparation. Apparently, no treatment or vaccine until now has been discovered; nevertheless, the reposition of the currently approved antiviral drugs are in use. Special consideration for more susceptible people such as elderly people, patients with chronic diseases, and health care takers should be applied. This study is considered as a serious attempt to synthesize a unique series of oleanolic acid (1) and maslinic acid (2) derivatives as anti-SARS-CoV-2 candidates. In this study, the ultrasound-assisted extraction of triterpenoids 1 and 2 from pomace olive of Olea europaea L. was optimized compared to previous methods used. The triterpenoid yield was 3.6 and 9.2 mg/g dry weight (DW), respectively, under optimal conditions. As shown in Table 1, the present study is the first report on the simultaneous extraction of triterpenoids 1 and 2 from pomace olive (Olea europaea L.) cultivar: Chemlali with a large amount under green chemistry conditions (ultrasonic-assisted extractions, then centrifugation, and purification), compared with those of previous works. This developed process could be useful in the preparation of a triterpenoid-rich ingredient that holds great promise for application in the therapeutic industry. To prepare the propargylated ester 3, derived from oleanolic acid 1, we started by the protection of the hydroxyl group at C-3 via its oxidation in order to direct the propargylation towards the carboxylic acid function in C-17 (Scheme 1). Then, we subjected the oxidized intermediate to the propargylation in dry N,N-Dimethylformamide (DMF) at room temperature in the presence of NaH and propargyl bromide for 2 h, yielding the desired propargylated intermediate which was reduced with sodium borohydride under microwave irradiation, to obtain the propargyl-(3β)-3-hydroxyolean-12-en-28-oate (3).

Cultivar
Extraction Methods Yield of Extraction (mg/g DW) Referen Oleanolic Acid 1 Maslinic Acid 2 Picual Solid-liquid extraction (maceration) To prepare the propargylated ester 3, derived from oleanolic acid 1, we started b protection of the hydroxyl group at C-3 via its oxidation in order to direct the propar tion towards the carboxylic acid function in C-17 (Scheme 1). Then, we subjected the dized intermediate to the propargylation in dry N,N-Dimethylformamide (DMF) at r temperature in the presence of NaH and propargyl bromide for 2 h, yielding the de propargylated intermediate which was reduced with sodium borohydride under m wave irradiation, to obtain the propargyl-(3β)-3-hydroxyolean-12-en-28-oate (3). Similarly, maslinic acid 2 was subjected to the same reaction of propargylation, w out oxidation beforehand, yielding a mixture of differently propargylated compo which were separated over silica gel column chromatography to afford compound (Table 2) [16,17]. Similarly, maslinic acid 2 was subjected to the same reaction of propargylation, without oxidation beforehand, yielding a mixture of differently propargylated compounds which were separated over silica gel column chromatography to afford compounds 4-7 (Table 2) [16,17]. Table 2. Oleanolic acid (1), maslinic acid (2) and their derivatives 3-17 screened for their anti-SARS-CoV-2 activity in addition to N3 (the co-crystallized native inhibitor of SARS-CoV-2).
All the reactions were assisted by microwave irradiation avoiding toxic reagents and solvents. The products were obtained from the reaction mixture by simple purification in almost quantitative yields (Table 2). Table 2. Oleanolic acid (1), maslinic acid (2) and their derivatives 3-17 screened for their anti-SARS-CoV-2 activity in addition to N3 (the co-crystallized native inhibitor of SARS-CoV-2).
All the reactions were assisted by microwave irradiation avoiding toxic reagents and solvents. The products were obtained from the reaction mixture by simple purification in almost quantitative yields (Table 2). Table 2. Oleanolic acid (1), maslinic acid (2) and their derivatives 3-17 screened for their anti-SARS-CoV-2 activity in addition to N3 (the co-crystallized native inhibitor of SARS-CoV-2).

Molecular Docking Studies
By studying the binding pocket of the SARS-CoV-2M pro , it was found that the cocrystallized native inhibitor (N3) fitted inside its binding pocket of the main protease receptor asymmetrically. N3 is a designed inhibitor for SARS-CoV-2 M pro that was built from amino acids based on the M pro pocket amino acids and cannot used medicinally ( Table 2).
The docked N3 inhibitor (18) was stabilized inside the SARS-CoV-2 M pro pocket through a hydrogen bond formation with Thr26 amino acid at 3.08 A°. Additionally, the most promising cytotoxic compound (17) fitted inside the binding site of the SARS-CoV-2 M pro by three hydrogen bond formations, two of them with Thr24 at 2.71 and 3.19 A°, respectively, and the third one was formed with Thr26 at 3.28 A°. Moreover, compound (3) formed three hydrogen bonds with the previously mentioned two amino acids as well.
All the reactions were assisted by microwave irradiation avoiding toxic reagents a solvents. The products were obtained from the reaction mixture by simple purification almost quantitative yields ( Table 2).  (2)  All the reactions were assisted by microwave irradiation avoiding toxic reagents and solvents. The products were obtained from the reaction mixture by simple purification in almost quantitative yields ( Table 2).

Molecular Docking Studies
By studying the binding pocket of the SARS-CoV-2M pro , it was found that the cocrystallized native inhibitor (N3) fitted inside its binding pocket of the main protease receptor asymmetrically. N3 is a designed inhibitor for SARS-CoV-2 M pro that was built from amino acids based on the M pro pocket amino acids and cannot used medicinally ( Table 2).
Molecular docking of oleanolic acid (1), maslinic acid (2), and other oleanolic acid and maslinic acid derivatives (3-17) ( Table 2), and N3 inhibitor (18) into the main protease binding site was performed. Almost all the tested compounds (1-17) were stabilized inside the M pro binding site by variable scores and binding interactions with the amino acids of the receptor pocket. It is worth mentioning that, especially for both compounds 17 and 3, the obtained binding scores and modes were greatly similar to that of the docked N3 inhibitor (18), as depicted in Table 3. Additionally, the 2 D binding modes of the tested compounds (1-17) with the amino acids of the SARS-CoV-2 M pro pocket are presented in Supplementary  Table S2. All of the tested compounds (1-17) achieved promising binding scores (from −6.55 to −10.20 kcal/mol), compared to the docked co-crystallized N3 inhibitor (18) with a binding score of −9.70 kcal/mol. The RMSD_refine values of the selected poses were within the acceptable range (from 0.9 to 2.25).

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-

Compound 17 Showed Promising Anti-SARS-CoV-2 Activity at Non-Toxic Concentrations
To identify the proper concentrations to define the antiviral activity of the selected compounds, half maximal cytotoxic concentration "CC50" was calculated for each individual drug (Figure 4). The antiviral screening revealed that one compound "17" out of the tested panel compounds exhibits a promising in vitro activity against SARS-CoV-2 "NRC-The docked N3 inhibitor (18) was stabilized inside the SARS-CoV-2 M pro pocket through a hydrogen bond formation with Thr26 amino acid at 3.08 A • . Additionally, the most promising cytotoxic compound (17) fitted inside the binding site of the SARS-CoV-2 M pro by three hydrogen bond formations, two of them with Thr24 at 2.71 and 3.19 A • , respectively, and the third one was formed with Thr26 at 3.28 A • . Moreover, compound (3) formed three hydrogen bonds with the previously mentioned two amino acids as well. It formed two hydrogen bonds with Thr24 at 2.80 and 3.18 A • , respectively, and the third one with Thr26 at 3.37 A • . Additionally, it is worth mentioning that the achieved binding scores for compounds 17 and 3 were −8.00 and −7.55 kcal/mol, respectively, compared to the docked co-crystallized N3 inhibitor (18) (−9.70 kcal/mol) ( Table 3).
By considering the docking findings of the selected tested compounds (1-17) against the binding pocket of the SARS-CoV-2 main protease compared to its co-crystallized inhibitor (N3) as a reference standard, and its great matching with the cytotoxicity studies applied, we can build a very promising and recommended idea about their mechanism of action.

Compound 17 Showed Promising Anti-MERS-CoV Activity and Non-Toxic Concentrations
To assess the antiviral activity of compound 17 against other highly pathogenic coronaviruses, we assayed its activity against MERS-CoV virus "NRCE-HKU270". Interestingly, compound 17 showed promising antiviral activity against NRCE-HKU270 at micromolar concentrations ( Figure 5).

Compound 17 Showed Promising Anti-MERS-CoV Activ centrations
To assess the antiviral activity of compound 17 against oth naviruses, we assayed its activity against MERS-CoV virus " ingly, compound 17 showed promising antiviral activity agai cromolar concentrations ( Figure 5). By studying the relations between the structures of the s (1-17) and their cytotoxic IC50 values ( Figure 6) and using the as a way to relate their activities to their binding affinities as S inhibitors, we may conclude that; (a) The common shared steroidal nucleus of the selected tes peared to be very promising for their antiviral activities. M  [45] in Vero E6 cells using crystal violet assay. Inhibitory concentration 50% (IC 50 ) values were calculated using nonlinear regression analysis of GraphPad Prism software (version 5.01) by plotting log inhibitor versus normalized response (variable slope).

Structure-Activity Relationship (SAR) Study
By studying the relations between the structures of the selected tested compounds (1-17) and their cytotoxic IC 50 values ( Figure 6) and using the molecular docking results as a way to relate their activities to their binding affinities as SARS-CoV-2 main protease inhibitors, we may conclude that; (a) The common shared steroidal nucleus of the selected tested compounds (1-17) appeared to be very promising for their antiviral activities. Molecular docking revealed the great stabilization of the tested compounds inside the branched large pocket of SARS-CoV-2 main protease as a proposed mechanism of action. (b) The presence of OH groups at positions 2 and 3 of the steroidal nucleus seems to be very crucial for the antiviral activity. Most compounds (17, 3, and 10) containing both 2-and 3-OH groups achieved high to moderate activities (4.12-99.87 µM). Docking studies referred to the involvement of both OH groups in hydrogen bond formations with Thr24 and/or Thr26 amino acids of the binding pocket. (c) Compound (17) containing the isoxazole side chain with a p-chlorophenyl substitution (an electron withdrawing lipophilic group) achieved a very high cytotoxic activity (IC 50 = 4.12 µM). However, both compounds (16 and 15) containing the isoxazole side chain with a p-methoxyphenyl substitution (an electron donating hydrophilic group) achieved low cytotoxic activities (IC 50 = 218.8 and 523 µM, respectively). Therefore, the lipophilic p-chloro group is very important for the antiviral activity. Again, compound (16) containing both 2-and 3-OH groups showed a higher cytotoxic activity compared to compound (15) with only 3-OH group as mentioned before.
(d) The presence of the acetyl methylene group at positions 2 and/or 3 in compounds (5, 7, and 6) gave better cytotoxic activities compared to the heteroaromatic ring substitutions at the same positions in compounds (12, 14, and 13). (e) The cytotoxic activity of compound (17) containing the isoxazole side chain with a p-chlorophenyl substitution (IC 50 = 4.12 µM) was clearly higher than the respective same compound (10) with a phenyl triazole side chain instead (IC 50 = 75.31 µM).
tion (an electron withdrawing lipophilic group) achieved a very high cytotoxic activity (IC50 = 4.12 µM). However, both compounds (16 and 15) containing the isoxazole side chain with a p-methoxyphenyl substitution (an electron donating hydrophilic group) achieved low cytotoxic activities (IC50 = 218.8 and 523 µM, respectively). Therefore, the lipophilic p-chloro group is very important for the antiviral activity. Again, compound (16) containing both 2-and 3-OH groups showed a higher cytotoxic activity compared to compound (15) with only 3-OH group as mentioned before. (d) The presence of the acetyl methylene group at positions 2 and/or 3 in compounds (5, 7, and 6) gave better cytotoxic activities compared to the heteroaromatic ring substitutions at the same positions in compounds (12, 14, and 13). (e) The cytotoxic activity of compound (17) containing the isoxazole side chain with a pchlorophenyl substitution (IC50 = 4.12 µM) was clearly higher than the respective same compound (10) with a phenyl triazole side chain instead (IC50 = 75.31 µM).  Collectively, these studies greatly suggested the very promising affinities of the selected tested compounds (1-17) against SARS-CoV-2, especially compounds 17 and 3. Accordingly, we recommend such compounds for more advanced in vitro and in vivo studies to obtain an effective therapeutic against the pandemic SARS-CoV-2. Moreover, the aforementioned studied compounds could be used alone or in combinations with each other's against SARS-CoV-2.

Mechanism of Actions
The virucidal effect of the antiviral compound 17 against SARS-CoV-2 virus has been studied, and interference with viral adsorption and replication was demonstrated. Besides the virucidal effect of compound 17, it could efficiently impair viral replication and adsorption (Figure 7). other's against SARS-CoV-2.

Mechanism of Actions
The virucidal effect of the antiviral compound 17 against SARS-CoV-2 virus has been studied, and interference with viral adsorption and replication was demonstrated. Besides the virucidal effect of compound 17, it could efficiently impair viral replication and adsorption (Figure 7).  (2) from the Pomace Olive of Olea europaea L.

Green Chemistry Condition for Extraction and Purification of OA (1) and MA
Using cultivar Chemlali pomace olive resulting from olive fruit pressing as a natural substance, the most suitable conditions to carry out the extraction and purification of compounds 1 and 2 were explored. The optimal conditions were determined to be the ultrasonic power of 250 W, extraction temperature of 60 °C, ratio of liquid to solid of 30 mL/g, and 60 min extraction time, using aqueous ethanol (concentration of 70%) as the extracting solvent. After the evaporation of the ethanol under reduced pressure, the extract was precipitated at +4 °C for 48 h. After filtration, the extract was centrifuged at 5000 rpm/5 min, yielding a mixture of compounds 1 and 2 according to TLC analysis (13.5% (w/w)).The crude mixture was recrystallized from the mixture EtOH/AcOEt (8:2, v/v) to obtain pure maslinic acid 2 (9.2 mg/g DW). The filtrate was concentrated and recrystallized from ethanol to obtain pure oleanolic acid 1 (3.6 mg/g DW).

Synthesis
Multicomponent Synthesis of 1,5-Disubstituted Triazoles in Water Catalyzed by Cp*RuCl (PPh3)2 To a solution of alkyne 3 or 4 (0.1 g) and NaN3 (1.1 mmol) in water, Cp*RuCl(PPh3)2 (5 mol%) was added at room temperature. To this mixture, alkyl halide (1.0 mmol) was added, and the reaction mixture was subjected to microwave irradiations at 250 W until total conversion of the starting alkyne (TLC). The crude mixture was extracted with EtOAc (3 × 30 mL). The organic layer was dried over Na2SO4. After the removal of solvent under  Using cultivar Chemlali pomace olive resulting from olive fruit pressing as a natural substance, the most suitable conditions to carry out the extraction and purification of compounds 1 and 2 were explored. The optimal conditions were determined to be the ultrasonic power of 250 W, extraction temperature of 60 • C, ratio of liquid to solid of 30 mL/g, and 60 min extraction time, using aqueous ethanol (concentration of 70%) as the extracting solvent. After the evaporation of the ethanol under reduced pressure, the extract was precipitated at +4 • C for 48 h. After filtration, the extract was centrifuged at 5000 rpm/5 min, yielding a mixture of compounds 1 and 2 according to TLC analysis (13.5% (w/w)).The crude mixture was recrystallized from the mixture EtOH/AcOEt (8:2, v/v) to obtain pure maslinic acid 2 (9.2 mg/g DW). The filtrate was concentrated and recrystallized from ethanol to obtain pure oleanolic acid 1 (3.6 mg/g DW). To a solution of alkyne 3 or 4 (0.1 g) and NaN 3 (1.1 mmol) in water, Cp*RuCl(PPh 3 ) 2 (5 mol%) was added at room temperature. To this mixture, alkyl halide (1.0 mmol) was added, and the reaction mixture was subjected to microwave irradiations at 250 W until total conversion of the starting alkyne (TLC). The crude mixture was extracted with EtOAc (3 × 30 mL). The organic layer was dried over Na 2 SO 4 . After the removal of solvent under reduced pressure, the resulting residue was purified by recrystallization from ethanol, to afford the desired products 8 and 9 in 90% and 92% yields, respectively [46,47].

Multicomponent Synthesis of 3,5-Disubstituted Isoxazoles in Water Catalyzed by CuI
At room temperature and stirring, to a mixture of alkyl derivatives 3 or 4 (0.1 g), NH 2 OH.HCl (1.2 equiv) and 0.1 equiv copper(I) iodide (CuI) in water, the appropriate aldehyde (1.1 equiv) was added, and the mixture was subjected to microwave irradiations at 250 W for 5-10 min (Scheme 2). The crude mixture was diluted with water and then extracted with EtOAc (3 × 30 mL). The organic layer was dried over Na 2 SO 4 . After the removal of solvent in vacuo, the resulting residue was purified by recrystallization from ethanol, to obtain the desired products 15-17 in 98, 87 and 96% yield, respectively [49].
The spectral data of all the synthesized compounds are in agreement with the literature data [16][17][18]50].

Molecular Docking Studies
Molecular docking studies using MOE 2019.012 suite [51] were performed to propose the expected mechanism of action for the 17 tested compounds as SARS-CoV-2 M pro inhibitors through evaluating their binding scores and interaction modes at the SARS-CoV-2 M pro receptor active site. The co-crystallized native inhibitor (N3) was extracted from the protein complex and inserted in our calculations as a reference standard.

Preparation of the Active Site of the Applied SARS-CoV-2 M pro Receptor
The Protein Data Bank website was used to extract the X-ray structure of the SARS-CoV-2 M pro receptor (PDB ID: 6LU7) [53]. Then, all default steps for its preparation were run as described earlier in detail [54,55].

Validation of the Applied MOE Program
Before applying the docking process, we first performed a validation process for the prepared target SARS-CoV-2 M pro receptor by redocking its co-crystallized native inhibitor (N3) inside its active site and comparing the resulted RMSD values between the co-crystallized (native) and docked N3 molecules. The obtained low RMSD value (1.20) indicated a promised valid performance (Supplementary Table S1) [56].

Docking of the Prepared Database to the Binding Pocket of SARS-CoV-2 M pro Receptor
The previously discussed database was selected and inserted at the site of ligand at the beginning of the general docking process and the default methodology was performed as described before [57]. Finally, we selected the best poses according to their binding scores, RMSD values, and binding modes for further considerations.

In Vitro Antiviral Bioassay Cytotoxicity (CC 50 ) Determination
To assess the half maximal cytotoxic concentration (CC 50 ), stock solutions of the test compounds were prepared in 10% DMSO in ddH 2 O and diluted further to the working solutions with DMEM. The cytotoxic activity of the extracts was tested in VERO-E6 cells by using crystal violet assay as previously described [58,59] with minor modifications. Briefly, the cells were seeded in 96-well plates (100 µL/well at a density of 3 × 105 cells/mL) and incubated for 24 h at 37 • C in a humidified 5% CO 2 incubator. After 24 h, cells were treated with various concentrations of the tested compounds in triplicates. Seventy two hours later, the supernatant was discarded, and cell monolayers were fixed with 10% formaldehyde for 1 h at room temperature (RT). The fixed monolayers were then dried and stained with 50 µL of 0.1% crystal violet for 20 min on a bench rocker at RT. The monolayers were then washed, dried and the crystal violet dye in each well was then dissolved with 200 µL of methanol for 20 min on a bench rocker at RT. The absorbance of crystal violet solutions was measured at λmax 570 nm as a reference wavelength using a multi-well plate reader. Cytotoxic concentration 50% (CC 50 ) values were calculated using nonlinear regression analysis of GraphPad Prism software (version 5.01) by plotting log inhibitor versus normalized response (variable slope).

Inhibitory Concentration 50 (IC 50 ) Determination
The IC 50 values for the tested compounds were determined as previously described [2,3], with minor modifications. Briefly, in 96-well tissue culture plates, 2.4 × 10 4 Vero-E6 cells were distributed in each well and incubated overnight at a humidified 37 • C incubator under 5% CO 2 condition. The cell monolayers were then washed once with 1× PBS. An aliquot of the SARS-CoV-2 "NRC-03-nhCoV" virus [40] containing 100 TCID50 was incubated with serial diluted compounds and kept at 37 • C for 1 h. The Vero-E6 cells were treated with virus/compound mix and co-incubated at 37 • C in a total volume of 200 µL per well. Untreated cells infected with virus represented the virus control; however, cells that were not treated and not infected were the cell control. Following incubation at 37 • C in 5% CO 2 incubator for 72 h, the cells were fixed with 100 µL of 10% paraformaldehyde for 20 min and stained with 0.5% crystal violet in distilled water for 15 min at RT. The crystal violet dye was then dissolved using 100 µL of absolute methanol per well and the optical density of the color was measured at 570 nm using the Anthos Zenyth 200 rt plate reader (Anthos Labtec Instruments, Heerhugowaard, the Netherlands). The IC 50 of the compound was that required to reduce the virus-induced cytopathic effect (CPE) by 50%, relative to the virus control.

Mechanism of Action(s) for Compound 17
To investigate whether the tested compound with high selectivity index and antiviral activity against NRC-03-nhCoV affect (a) viral adsorption, (b) viral replication or (c) viricidal effect, a plaque infectivity reduction assay was performed according to the following tprotocols: (a) Viral adsorption mechanism The viral adsorption mechanism was assayed according to a protocol by Zhang et al. [60] with minor modifications. Vero-E6 cells were cultivated in a 6-well plate (10 5 cells/mL) for 24 h at 37 • C. Each tested drug was applied in 500 µL of medium without supplements and co-incubated with the cells for 2 h at 4 • C. The non-absorbed drug in the applied inocula was removed by washing cells three times with 1x PBS. The NRC-03-nhCoV was diluted to a countable range "10 −4 " and co-incubated with the pretreated cells. About 1 h post-incubation, the inocula were removed and 3 mL of agarose overlay (1:1 mixture of warmed 2× plaque media and a stock solution of heated 2% agarose) were added. The plates were kept at room temperature to solidify and then incubated at 37 • C to allow the formation of viral plaques. The plaques were fixed using 10% formaldehyde, stained using crystal violet (1% crystal violet in 20% ethanol and distilled H 2 O), washed with rinse water and dried to calculate the percentage reduction in plaque formation compared to control wells, which comprised untreated Vero-E6 cells directly infected with NRC-03-nhCoV.

(b) Viral replication mechanism
The impact of the tested drug on viral replication was determined as previously described [50]. Vero-E6 cells were cultivated in a 6-well plate (10 5 cell/mL) for 24 h at 37 • C. The NRC-03-nhCoV of the countable range was inoculated directly to the cells and incubated for 1 h at 37 • C. The inocula containing the non-adsorbed virus were removed by washing infected cell monolayers three times with 1× PBS. The analyzed compound was then applied in varying concentrations to infected cells for another 1 h contact time at 37 • C. After removing the inocula containing the tested drug, agarose overlay were added to the cells. The plates were kept at room temperature to solidify and then incubated at 37 • C to allow the formation of viral plaques. The plaques were fixed using 10% formaldehyde, stained using 1% crystal violet washed with rinse water and dried to calculate the percentage reduction in plaque formation compared to control wells, which comprised untreated Vero-E6 cells directly infected with NRC-03-nhCoV.
(c) Virucidal mechanism The virucidal mechanism was assayed following a previously described protocol [61]. Briefly, Vero-E6 cells (105 cells/mL) were cultivated for 24 h at 37 • C in a 6-well plate. In an eppendorf, 200 µL of stock NRC-03-nhCoV were mixed with the tested compound at different concentrations. The mixture was kept for 1 h at room temperature against the control untreated virus. After 1 h of incubation, the mixture was ten-fold serial diluted four times using serum-free medium to obtain a countable range of the virus. An aliquot of 100 µL of each dilution were added to the Vero-E6 cell monolayer. After 1 h contact time, the agarose overlay was added to the cell monolayer. The plates were kept at room temperature to solidify and then incubated at 37 • C to allow the formation of viral plaques. The plaques were fixed using 10% formaldehyde, stained using 1% crystal violet washed with rinse water and dried to calculate the percentage reduction in plaque formation compared to control wells, which comprised untreated Vero-E6 cells directly infected with NRC-03-nhCoV.

Conclusions
Oleanolic acid (1) and maslinic acid (2), isolated from pomace olive oil, and a series of their synthesized structural analogues (3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) were first evaluated using molecular docking for their proposed expected inhibitory activities against SARS-CoV-2 main protease, and then were screened for the same activity. Most of the tested compounds showed promising affinities towards SARS-CoV-2 main protease, especially for the most active member (17). Accordingly, we recommend such compounds for more advanced in vitro and in vivo studies to obtain an effective therapeutic against the pandemic SARS-CoV-2. Moreover, the aforementioned studied compounds could be used alone or in combinations with each other against SARS-CoV-2.

Patents
This work presented in this manuscript is submitted as a patent application to Saudi Authority for Intellectual Property under number 121420640.