Novel Sophoridine Derivatives as Potential Larvicidal Agents against Aedes albopictus: Synthesis, Biological Evaluation, Acetylcholinesterase Inhibition, and Morphological Study

Simple Summary Sophoridine, a natural quinolizidine alkaloid with biological activities, was isolated and identified from traditional medicinal herbs. Although mosquitoes can be effectively controlled by chemical insecticides, their intense use has significantly increased the development and spread of resistant mosquitoes. Chemical insecticides have negative effects, such as potential health risks, water contamination, environmental pollution, and toxicity to nontarget organisms, including people. In this study, two series of sophoridine derivatives were generated using sophoridine as the lead compound. All derivatives were characterized and screened for their insecticidal activity against larva and adult mosquitoes. Their effects on the growth cycle of larva and their action on AChE from Aedes albopictus were also explored. Finally, morphological studies of the derivative-treated dead larvae were conducted. Abstract Two series of novel sophoridine derivatives were designed, synthesized, and evaluated for their anti-mosquito activity. SOP-2g, SOP-2q, and SOP-2r exhibited potential larvicidal activity against Aedes albopictus larva with LC50 values of 330.98, 430.53, and 411.09 ppm, respectively. Analysis of structure–activity relationships indicated that the oxime ester group was beneficial for improving the larvicidal biological activity, whereas the long-chain aliphatic group and fused-ring group were introduced. Furthermore, the larvicidal mechanism was also investigated based on the inhibition assay of acetylcholinesterase (AChE) and the morphological observation of dead larva treated with derivatives. Results indicated that the AChE inhibitory activity of the preferred three derivatives were 63.16%, 46.67%, and 35.11%, respectively, at 250 ppm concentration. Additionally, morphological evidence demonstrated that SOP-2q and SOP-2r induced changes in the larva’s intestinal cavity, caudal gill, and tail, thereby displaying larvicidal action against Ae. albopictus together with AChE inhibition. Therefore, this study implied that sophoridine and its novel derivatives could be used to control the population of mosquito larva, which may also be effective alkaloids to reduce the mosquito population density.


Introduction
Mosquitoes such as Aedes albopictus, Culex quinquefasciatus, and Anopheles gambiae act as vectors that transmit pathogens and cause parasitic and viral infectious diseases (e.g., malaria, dengue, yellow fever, Japanese encephalitis, Chikungunya, West Nile virus, Data for SOP-2: 1

General Procedure for the Synthesis of SOP-2a-SOP-2r
A 200 mL round-bottom flask was charged with HOBT (212.2 mg, 1.0 mmol) and EDCI (191.7 mg, 1.0 mmol), and then 15 mL of DCM was added, dissolved completely, and stirred at room temperature for 3 min. Afterwards, SOP-2 (150.0 mg, 0.5 mmol) was added and stirred for 3 h at room temperature. The reaction was monitored by TLC. The reaction mixture was poured into the water slowly (100 mL) and then extracted with ethyl acetate (3 × 40 mL). The organic phases were combined and dried with anhydrous MgSO 4 , filtered, and concentrated under reduced pressure to obtain the crude product. The residue was purified by silica-gel column chromatography (eluent: V MeOH /V DCM ) = 1/8) to obtain SOP-2a-SOP-2r [31][32][33]. The compounds were produced in 68-94% yields, and their structures were determined with the aid of 1 H and 13 C NMR and high-resolution electrospray-ionization mass spectrometry (HRESIMS) data. Data for SOP-2a and SOP-2b are presented here, whereas those for SOP-2c-SOP-2r are in the Supporting Information (File S1).
Data for SOP-2a: The compound was obtained in 80% yield as a white solid. 1 13  Data for SOP-2b: The compound was obtained in 72% yield as a white solid. 1 13  2.6. General Procedure for the Synthesis of SOP-3 K 2 CO 3 (107.0 mg, 0.8 mmol) was added to a solution of 200 mg SOP-2 dissolved completely with 5 mL of DCM in a round-bottomed flask. The solution was stirred for 1 min. Then, m-CPBA (134.0 mg, 0.8 mmol) was added and the mixture was set in an ice bath. The reaction system was gradually returned to room temperature and stirred. TLC was applied to monitor the reaction. After 10 h, the mixture was suction filtered to remove excess K 2 CO 3 and m-CPBA to obtain a crude product. This product was purified by silica-gel column chromatography (eluent: V MeOH /V DCM = 1/8) to obtain a white solid (SOP-3) with 90% yield [34], as shown in Scheme 1.

General Procedure for the Synthesis of SOP-3a-SOP-3g
HOBT (390.5 mg, 1.8 mmol), EDCI (352.7 mg, 1.8 mmol), pelargonic acid (142.4 mg, 0.9 mmol), and 15 mL of DCM were added into a 200 mL round-bottom flask, and the mixture was stirred at room temperature for 3 min. Then, SOP-3 (292.6 mg, 0.9 mmol) was added into the reaction vessel under stirring at room temperature and monitoring by TLC. After reacting for 3 h, the mixture was treated with water and extracted with ethyl acetate three times. The organic phase was combined, dried, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product and purified by silica-gel column chromatography (eluent: VMeOH/VDCM = 1/30) to obtain SOP-3a-SOP-3f [31][32][33]. These compounds were synthesized in 54-85% yields, and their structures were established by a combination of 1 H and 13 C NMR and HRESIMS data. Data for SOP-3a and SOP-3b are presented here, whereas those for SOP-3c-SOP-3f are in the Supporting Information.
Data for SOP-3a: The compound was obtained in 54% yield as a yellow solid. 1 13  Data for SOP-3b: The compound was obtained in 56% yield as a yellow solid. 1

General Procedure for the Synthesis of SOP-3a-SOP-3g
HOBT (390.5 mg, 1.8 mmol), EDCI (352.7 mg, 1.8 mmol), pelargonic acid (142.4 mg, 0.9 mmol), and 15 mL of DCM were added into a 200 mL round-bottom flask, and the mixture was stirred at room temperature for 3 min. Then, SOP-3 (292.6 mg, 0.9 mmol) was added into the reaction vessel under stirring at room temperature and monitoring by TLC. After reacting for 3 h, the mixture was treated with water and extracted with ethyl acetate three times. The organic phase was combined, dried, and filtered. The filtrate was concentrated under reduced pressure to obtain the crude product and purified by silica-gel column chromatography (eluent: V MeOH /V DCM = 1/30) to obtain SOP-3a-SOP-3f [31][32][33]. These compounds were synthesized in 54-85% yields, and their structures were established by a combination of 1 H and 13 C NMR and HRESIMS data. Data for SOP-3a and SOP-3b are presented here, whereas those for SOP-3c-SOP-3f are in the Supporting Information.
Data for SOP-3a: The compound was obtained in 54% yield as a yellow solid. 1 13  Data for SOP-3b: The compound was obtained in 56% yield as a yellow solid. 1 13  According to the literature [35], each derivative was dissolved in acetone with a final concentration of 1000 ppm. An appropriate amount of live Ae. albopictus fourth-instar larvae (dark gray and about 7 mm long) were selected and washed two to three times with deionized water. A 24-well plate with a test well and four replication wells for each derivative was used, and each well had five larvae in it. Extra deionized water was removed with a pipette, and then 985 µL of clean deionized water and 5 µL of feed solution containing 25 mg/mL was added. Finally, about 10 µL of derivative solution was added. Deltamethrin (1000 ppm) and acetone replaced the derivative as positive and negative control groups, respectively. Three independent replicate tests were performed. The 24-well plate was cultivated in an incubator maintained at a constant temperature of 28 • C and 80% relative humidity under light and dark conditions for 12 h each. After 24 h, the lethality of each derivative to the larva was recorded if the larva did not move by using the pipette tip to touch the larva's body, at which point it was assumed to be dead.
After the pre-experiment screening, derivatives with a lethality of at least 50% were chosen to participate in the semi-inhibitory concentration test. Each derivative was diluted with acetone to obtain five to nine concentration gradients ranging from 100 ppm to 1000 ppm. Subsequently, the above procedure was repeated, and the lethality of each derivative to the larva was recorded. According to the relationship between the concentration of sophoridine derivatives and mortality, the virulence regression equation was established. The LC 50 value was calculated according to the equation below, and the larvae in the blank control group should not die off more than 5% given the variations in each individual: Mortality rate (%) = A1/B1 × 100% A1: number of larvae killed at each concentration on the 24-well plate; B1: initial number of larvae added per concentration on the 24-well plate.

Insecticidal Tests for Female Mosquitoes of Ae. albopictus
In order to determine the insecticidal activity toward female mosquitoes of Ae. albopictus, a 0.25 µL (equivalent to 0.25 µg/female) of the 1000 ppm aforementioned derivative solution was topically dropped onto the dorsum of adult mosquitoes with a handheld pipetting device [36,37]. The derivatives were dissolved in acetone, a highly volatile substance that stayed on the epidermis of adult mosquitoes for a short period of time. Twenty-five susceptible female mosquitoes that were 2-5 days after eclosion and had not sucked blood were collected into each test cup. The female mosquito was lightly anesthetized with CO 2 for 15-30 s and then placed on a cold table cooled to 4 • C in advance. A quick operation ensured that the mosquito remained anesthetized during the procedure. Three parallel groups were made for each test, and the female mosquitoes were treated with 0.25 µL of pure acetone as a negative control. After drug administration, the mosquitoes were transferred into another test cup with cotton moistened with a 10% sugar water solution and placed on the top of the test cup. Mortality was recorded after 24 h of rearing at 26-28 • C, 80% relative humidity, and light/dark (12 h/12 h). Deltamethrin (0.25 µg/female) and acetone were used as positive and negative control groups, respectively. Importantly, the mortality rate of the negative control group did not exceed 5%. Three sets of repeated tests were conducted for different batches of adult mosquitoes. Finally, the lethality rate of the derivatives for adult mosquitoes was calculated by the following formula: Lethality rate (%) = A2/B2 × 100% A2: number of female adult mosquitoes killed in each test cup; B2: number of female adult mosquitoes added into each test cup. Compounds SOP-2g, SOP-2q, and SOP-2r, which had better larvicidal activity than other sophoridine derivatives, were screened out to investigate their effects on the emergence of Ae. albopictus larvae. The final test concentration of the derivative was set at LC 30 according to the result of the LC 50 test and using the high-throughput screening method [38,39]. A total of 5 fourth-instar larvae of Ae. albopictus with 985 µL of deionized water, 10 µL of sample solution, and 5 µL of feed solution were added to each well on a 24-well plate and incubated under constant temperature and humidity for 24 h. Eight replicate wells were set for each concentration, and three independent replicate experiments were performed. After culturing for 24 h, the still-surviving larvae were sucked out with a dropper and rinsed with deionized water two to three times. Afterwards, the larvae were transferred onto a new 24-well plate, with one treated larva placed in each well with 985 µL of deionized water, 10 µL of the same sample solution, and 5 µL of feed solution added. The 24-well plate of larvae treated with the same compound was laid into a mosquito cage, and 10% sugar water was handed into each cage concurrently. The condition of the rearing environment was set at 28 • C, the relative humidity was 80%, and it was light and dark for 12 h each. Finally, the growth state of the larvae was observed every 12 h from the day the larvae were placed into the mosquito cage. The status of the larvae was recorded with the following scores: 0, death; 4, larva; 5, pupa; 6, adult mosquito; 4-0, death as larva; 5-0, death as pupa; and 6-0, death as an adult mosquito.

Effects on the Fertility of Female Adult Mosquitoes of Ae. albopictus
The mosquitoes, evolving from the larvae that survived in the above experiments of effects on the emergence of larvae, were starved for 24 h. They were then fed with blood, and the abdomen of the female mosquitoes was clearly bulging and blood red after blood sucking [40]. At this time, the female mosquitoes were transferred into new cages by using a manual suction device. Five female mosquitoes, an egg-collection device, and a water-feeding device were placed inside each cage. The average number of eggs laid by females was used as an indicator to calculate the fertility of females with the formula below: Average number of eggs laid (%) = number of eggs on oviposition paper/number of females laying eggs × 100%

Inhibitory Activity of Compounds against Larval Enzyme
According to the reported method [41], 50 fourth-instar larvae of Ae. albopictus to be tested were initially aspirated with a dropper. The larvae were washed twice with deionized water, and the water on the surface of the larvae was blotted with filter paper. Second, the larvae were transferred into a 1.5 mL centrifuge tube. Then, 0.6 mL of ice-cold 0.1 M PBS buffer (pH 8.0) was added to the tube and ground in an ice bath with a grinding rod for 30 s. Third, the larvae were sonicated 20 times at low temperature with an ultrasonic crusher. Finally, the tube was centrifuged at 1700× g for 15 min at 4 • C to remove the larval tissue fragments, and the supernatant was aspirated as the AchE solution to be tested. Then, AchE in the fourth-instar larvae of Ae. albopictus was determined based on the Ellman method. The sophoridine derivatives SOP-2g, SOP-2q, and SOP-2r were diluted into different concentration gradients in which 1 µL of each sample of concentration gradient was dropped onto a 96-well plate. The negative control group was 1 µL of acetone solution. Subsequently, 79 µL of AchE solution was pipetted onto a 96-well plate and shaken at room temperature for 30 s to mix well. After the mixture was incubated at 28 • C for 10 min, 10 µL of 5,5 -dithiobinitrobenzoic acid solution and 10 µL of acetylcholine iodosulfide solution were added rapidly under light-proof conditions. Then, the 96-well plate was immediately placed into the enzyme standard to determine the OD value at a 412 nm wavelength, and the OD value was measured every 1 min for 30 min. The activity was calculated by the following formula: where T is the specific activity of enzyme in the treatment group and CK is the specific activity of the enzyme in the control group.
Specific activity of enzyme = enzyme activity unit/enzyme source protein content Activity unit of enzyme = ∆OD 412 × V/ε × L where ∆OD 412 is the change in absorbance per minute (∆OD 412 /min); V is the reaction system (µL); ε is the extinction coefficient [0.0136 L/µmoL/cm]; and L is the light range (1 cm).

Observation of Morphological Changes in Dead Larvae
The morphology of instar larvae was observed according to a reported method [42]. First, the fourth-instar larvae of Ae. albopictus were treated with the LC 90 concentration of sophoridine derivatives (SOP-2q and SOP-2r) for 24 h, whereas the control group was replaced with acetone. Second, three larvae were collected from each treatment group for morphological analysis and image acquisition. Third, the larvae were mounted onto glass slides and observed by an IX73 inverted fluorescence microscope (magnification, 4 × 10 and 8 × 10). Finally, the larval head, thorax, abdomen, and other organs such as tail gills, siphons, segments, antennae, eyes, and other organs were compared and observed, and images were collected.

Chemistry
A total of 27 derivatives, including three intermediates (SOP-1-SOP-3) and 24 target compounds, were prepared using commercially available sophoridine (SOP) as the starting material, as described in Scheme 1. The target derivatives were divided into two series, which were obtained through a common two-step procedure. This procedure involved synthesizing the key intermediates of SOP-1 by the Vilsmeier-Haack reaction of SOP with DMF in the presence of POCl 3 , and SOP-2 was obtained by the reaction of compound SOP-1 with NH 2 OH under the basic condition in MeOH/H 2 O. Then, SOP-2a-SOP-2r were synthesized by the reaction of compound SOP-2 with different carboxylic acids (RCOOH) in good to excellent yields (68-94%). Additionally, N-oxide (SOP-3) was prepared by the oxidation of nitrogen atoms at position 1 in SOP-2 by using mCPBA. Subsequently, the target compounds (SOP-3a-SOP-3f) were obtained using the same method by the reaction of compound SOP-3 with different carboxylic acids (RCOOH) in 54-85% yields. All compounds were purified by silica-gel column chromatography, and TLC was used to monitor the experimental process. All products were characterized by FT-IR, 1 H NMR, 13 C NMR, and mass spectrometry.

Insecticidal Tests for Larval and Female Mosquitoes
Larvicidal activities against the fourth-instar larvae of Ae. albopictus of the synthesized compounds were investigated, and the structures of sophoridine and its derivatives with their mortalities are depicted in Table 1. The mortalities of the compounds at 1000 ppm ranged from 0% to 100%, which suggested that larvicidal activities may vary substantially with structural modifications. Oxime ester moiety is one of the most important fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electronwithdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment.

SOP-2a
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 18.33 ± 4.08%

SOP-2b
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 20.00 ± 6.12%

SOP-2n
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 13.00 ± 0.00%

SOP-2c
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 10.00% ± 3.12%

SOP-2d
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment.

SOP-2e
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 20.00 ± 0.00%

SOP-2q
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment.

SOP-2f
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 85.00 ± 1.67%

SOP-2g
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment.

SOP-2h
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 38.00 ± 6.10%

SOP-3b
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. 25.00 ± 1.45%

SOP-2i
Insects 2023, 14, x FOR PEER REVIEW 9 of 20 fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. fragments in organic chemistry and exists in lots of bioactive compounds that show a large number of bioactivities, such as antifungal, antioxidant, and insecticidal activities [43,44]. New matrine-type alkaloid analogs were designed by the introduction of oxime ester fragments into matrine at its C14 position and exhibited excellent agricultural activities against two threatening pests, Tetranychus cinnabarinus Boisduval and Mythimna separata Walker [45]. Therefore, this result suggested the importance of introducing the amidoxime ester fragment at the C14 position of sophoridine, because the mortalities of the derivatives were higher than those of the parent compound, especially compounds SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f, with inhibition rates ranging from 80% to 100%. These values were much higher than the 10% inhibition rate of the parent compound. Additionally, the mortalities were lower than 50% when benzene ring structures, either with an electron-withdrawing group or an electron-donating group, were introduced into the oxime ester fragment. Meanwhile, when a long aliphatic alkyl chain with nine carbons or ten carbons was introduced, the inhibition rates increased to 85-100%, which were significantly higher than those of the parent compound. This finding indicated that α-naphthalene was an effective substitution to improve the larvicidal activity because the mortality reached 90% when the ligand was introduced into the oxime ester fragment. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC 50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution. Results of preliminary activity tests for all compounds against female Ae. albopictus mosquito are shown in Table 2. Sophoridine and its derivatives had low activity against adult mosquitoes, with the highest mortality of 35.27% less than 50% at test concentration. Therefore, the test of LC50 concentration for female mosquitoes of Ae. albopictus was not conducted in this study. The differences between larvae and female adult mosquitoes may be explained by the topical application of the compounds to evaluate insecticidal activity against adult mosquitoes. These compounds were required to infiltrate the epidermis and enter the adult mosquitoes, causing their disability or death. Conversely, larvicidal activity was tested by the microporous plate method, in which compounds were dissolved in water and entered the larva directly through feeding. However, the way of premedication could affect the results of insecticidal activity. For these series of SOP derivatives, their high polarity and hydrophilicity could lead to activity bias toward the larvae rather than the adult. Additionally, the bioassay conditions for larvae and adult mosquitoes are different. For example, whole larvae were immersed in 1000 ppm solutions; however, the female adult only topically accepted 0.25 µL 1000 ppm solution.  According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site. 26

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site. 25

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

SOP-2k
Insects 2023, 14 According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site. According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

SOP-2l
Insects 2023, 14 According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site. 24 According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC20, LC50, and LC90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit. Compounds SOP-2g, SOP-2r, and SOP-2q possessed high lethality (<500 ppm) to larvae, with LC50 values of 330.98, 411.09, and 430.53 ppm, respectively, whereas the LC50 of SOP-3f was 546.68 ppm. Similarly, the LC50 of the intermediate compound SOP-2 was 527.01 ppm, which was lower than that of the intermediate compound SOP-3 of 594.03 ppm. Overall, these results demonstrated that the N1 position of the parent compound sophoridine was an important active site.

Dose-Response Curves on Ae. albopictus Larvae
According to the result of preliminary activity tests on Ae. albopictus larvae, the doseresponse curves of six selected sophoridine derivatives (SOP-2, SOP-2g, SOP-2q, SOP-2r, SOP-3, and SOP-3f) were determined with the expanded concentration test range of the compounds, as shown in Figure 1. Results indicated that the insecticidal efficacy on Ae. albopictus larvae showed a dose-dependent manner. As shown in Table 3, the LC 20 , LC 50 , and LC 90 values on Ae. albopictus larvae were calculated according to the toxicity regression equations generated from the dose-response curves, and the R 2 values ranged from 0.970 to 0.993, which were close to 1, indicating a good fit.     As shown in Figure 2, the effects of sophoridine derivatives (SOP-2g, SOP-2q, and SOP-2r) on larval emergence were tested and compared with the negative control group. The eclosion of the larvae in the negative control group started on the third day with a rate of 15%, whereas the larvae eclosion in the SOP-2g and SOP-2r treatment groups started on the fourth day with rates of 4% and 7%, respectively, which were five and three times less than that of the negative control group. The eclosion of the larvae treated with SOP-2q was further delayed and started only on the fifth day with an eclosion rate of 11%, whereas the eclosion rate of the negative control group reached 33% at this time point. The selected sophoridine derivatives delayed the emergence time and reduced the emergence rate of larvae of Ae. albopictus. Additionally, the mortalities of compound-treated groups increased in the first 3 and 5 days, in which the mortality rate of the SOP-2g treatment group was 7%, and those of the SOP-2r and SOP-2q treatment groups reached 13% and 15%, respectively. Meanwhile, no death occurred in the negative control group. Finally, the mortality rates of the test groups stabilized after 3 and 5 days in this experiment. These mortalities suggested that the chronic toxicity of the selected sophoridine derivatives caused the larvae to fail to transform into pupae and emerge successfully. Therefore, the result of the emergence experiment indicated the possibility of sustaining mosquito control by delaying the emergence time and reducing the emergence rate of larvae. As shown in Figure 2, the effects of sophoridine derivatives (SOP-2g, SOP-2q, and SOP-2r) on larval emergence were tested and compared with the negative control group. The eclosion of the larvae in the negative control group started on the third day with a rate of 15%, whereas the larvae eclosion in the SOP-2g and SOP-2r treatment groups started on the fourth day with rates of 4% and 7%, respectively, which were five and three times less than that of the negative control group. The eclosion of the larvae treated with SOP-2q was further delayed and started only on the fifth day with an eclosion rate of 11%, whereas the eclosion rate of the negative control group reached 33% at this time point. The selected sophoridine derivatives delayed the emergence time and reduced the emergence rate of larvae of Ae. albopictus. Additionally, the mortalities of compound-treated groups increased in the first 3 and 5 days, in which the mortality rate of the SOP-2g treatment group was 7%, and those of the SOP-2r and SOP-2q treatment groups reached 13% and 15%, respectively. Meanwhile, no death occurred in the negative control group. Finally, the mortality rates of the test groups stabilized after 3 and 5 days in this experiment. These mortalities suggested that the chronic toxicity of the selected sophoridine derivatives caused the larvae to fail to transform into pupae and emerge successfully. Therefore, the result of the emergence experiment indicated the possibility of sustaining mosquito control by delaying the emergence time and reducing the emergence rate of larvae.

Effects on the Fertility of Female Adult Ae. albopictus Mosquitoes
To explore the effect of SOP derivatives on the fecundity of Ae. albopictus, the average number of eggs laid by the adult mosquitoes that emerged from the SOP derivativetreated larvae was recorded. Results are shown in Figure 3, where it could be found that

Effects on the Fertility of Female Adult Ae. albopictus Mosquitoes
To explore the effect of SOP derivatives on the fecundity of Ae. albopictus, the average number of eggs laid by the adult mosquitoes that emerged from the SOP derivative-treated larvae was recorded. Results are shown in Figure 3, where it could be found that the average egg-laying rate of the treated female mosquitoes did not change significantly compared with that of the control group. Thus, the derivatives had no obvious effect on the fecundity of female Ae. albopictus mosquitoes. the average egg-laying rate of the treated female mosquitoes did not change significantly compared with that of the control group. Thus, the derivatives had no obvious effect on the fecundity of female Ae. albopictus mosquitoes.

SARs
The SAR analysis results of these novel sophoridine derivatives are summarized in Figure 4. First, the insecticidal activity against the larvae of Ae. albopictus significantly increased when the oxime ester fragment was introduced at the C14 position of sophoridine, whereas the parent compound showed low insecticidal activity. Second, a series of sidechain structures was introduced by esterification at the hydroxyl group of the oxime ester. Anti-mosquito activity did not improve when the side-chain groups were benzene-ring structures with either electron-absorbing or electron-donating groups compared with the parent compound. However, the larvicidal activity of the derivatives significantly increased when aliphatic long carbon chains and fused-ring fragments were introduced. Third, it was found that the N1 position of the parent compound sophoridine was an important active site to the larvicidal activity.

SARs
The SAR analysis results of these novel sophoridine derivatives are summarized in Figure 4. First, the insecticidal activity against the larvae of Ae. albopictus significantly increased when the oxime ester fragment was introduced at the C14 position of sophoridine, whereas the parent compound showed low insecticidal activity. Second, a series of sidechain structures was introduced by esterification at the hydroxyl group of the oxime ester. Anti-mosquito activity did not improve when the side-chain groups were benzene-ring structures with either electron-absorbing or electron-donating groups compared with the parent compound. However, the larvicidal activity of the derivatives significantly increased when aliphatic long carbon chains and fused-ring fragments were introduced. Third, it was found that the N1 position of the parent compound sophoridine was an important active site to the larvicidal activity.
the average egg-laying rate of the treated female mosquitoes did not change significantly compared with that of the control group. Thus, the derivatives had no obvious effect on the fecundity of female Ae. albopictus mosquitoes.

SARs
The SAR analysis results of these novel sophoridine derivatives are summarized in Figure 4. First, the insecticidal activity against the larvae of Ae. albopictus significantly increased when the oxime ester fragment was introduced at the C14 position of sophoridine, whereas the parent compound showed low insecticidal activity. Second, a series of sidechain structures was introduced by esterification at the hydroxyl group of the oxime ester. Anti-mosquito activity did not improve when the side-chain groups were benzene-ring structures with either electron-absorbing or electron-donating groups compared with the parent compound. However, the larvicidal activity of the derivatives significantly increased when aliphatic long carbon chains and fused-ring fragments were introduced. Third, it was found that the N1 position of the parent compound sophoridine was an important active site to the larvicidal activity.

Inhibitory Activity of Compounds against AChE
The inhibition rates of the sophoridine derivatives (SOP-2g, SOP-2q, and SOP-2r) on AChE at different concentrations were tested, and results are shown in Figure 5. The inhibition rate of SOP-2g with 63.16% was higher than those of SOP-2q and SOP-2r, with 46.67% and 35.11%, respectively, at the highest concentration (250 ppm) of each compound. At the lowest concentration (50 ppm), SOP-2g remained the most potent analog with an inhibition rate of 44.72%. Intriguingly, the inhibitory activities against the larval AChE of these three sophoridine derivatives were generally concentration-dependent.
Thus, the insecticidal mechanism of the SOP derivatives could be partially mediated by inhibiting AChE. Insect poisoning or even death can result from the cholinergic system being destroyed or obstructed, which overstimulates larval neurons and causes them to produce excess levels of the neurotransmitter acetylcholine. Further studies are required to validate this hypothesis.
The inhibition rates of the sophoridine derivatives (SOP-2g, SOP-2q, and SOP-2r) on AChE at different concentrations were tested, and results are shown in Figure 5. The inhibition rate of SOP-2g with 63.16% was higher than those of SOP-2q and SOP-2r, with 46.67% and 35.11%, respectively, at the highest concentration (250 ppm) of each compound. At the lowest concentration (50 ppm), SOP-2g remained the most potent analog with an inhibition rate of 44.72%. Intriguingly, the inhibitory activities against the larval AChE of these three sophoridine derivatives were generally concentration-dependent. Thus, the insecticidal mechanism of the SOP derivatives could be partially mediated by inhibiting AChE. Insect poisoning or even death can result from the cholinergic system being destroyed or obstructed, which overstimulates larval neurons and causes them to produce excess levels of the neurotransmitter acetylcholine. Further studies are required to validate this hypothesis.

Observation of Larval Intestinal Cavity
The overall appearance of larvae in the negative control group was worm-like with eight obvious segments dividing the abdomen, as shown in Figure 6A. However, when the larvae were treated with the compounds, the abdomen segments became unclear, with multiple collapses laterally, and the abdomen became thinner and longer, as shown in Figure 6B,C. Moreover, the larvae of the negative control group had evenly distributed bristles, whereas the compound-treated groups had no continuous arrangement of bristles. According to the literature, the chemical toxicity of sophoridine derivatives may cause the deformation and hypertrophy of the intestinal epithelium of larvae, resulting in abdominal collapse. Then, the intestinal tract collapsed and noticeably darkened [46]. The first to fifth abdominal segments are in the midgut of the larva, playing a key role in the secretion of digestive enzymes [47]. Therefore, the imbalance in larval physiological function caused by the collapse of the intestinal lumen may explain the death of the larvae.

Observation of Larval Intestinal Cavity
The overall appearance of larvae in the negative control group was worm-like with eight obvious segments dividing the abdomen, as shown in Figure 6A. However, when the larvae were treated with the compounds, the abdomen segments became unclear, with multiple collapses laterally, and the abdomen became thinner and longer, as shown in Figure 6B,C. Moreover, the larvae of the negative control group had evenly distributed bristles, whereas the compound-treated groups had no continuous arrangement of bristles. According to the literature, the chemical toxicity of sophoridine derivatives may cause the deformation and hypertrophy of the intestinal epithelium of larvae, resulting in abdominal collapse. Then, the intestinal tract collapsed and noticeably darkened [46]. The first to fifth abdominal segments are in the midgut of the larva, playing a key role in the secretion of digestive enzymes [47]. Therefore, the imbalance in larval physiological function caused by the collapse of the intestinal lumen may explain the death of the larvae. hibition rate of SOP-2g with 63.16% was higher than those of SOP-2q and SOP-2r, with 46.67% and 35.11%, respectively, at the highest concentration (250 ppm) of each compound. At the lowest concentration (50 ppm), SOP-2g remained the most potent analog with an inhibition rate of 44.72%. Intriguingly, the inhibitory activities against the larval AChE of these three sophoridine derivatives were generally concentration-dependent. Thus, the insecticidal mechanism of the SOP derivatives could be partially mediated by inhibiting AChE. Insect poisoning or even death can result from the cholinergic system being destroyed or obstructed, which overstimulates larval neurons and causes them to produce excess levels of the neurotransmitter acetylcholine. Further studies are required to validate this hypothesis.

Observation of Larval Intestinal Cavity
The overall appearance of larvae in the negative control group was worm-like with eight obvious segments dividing the abdomen, as shown in Figure 6A. However, when the larvae were treated with the compounds, the abdomen segments became unclear, with multiple collapses laterally, and the abdomen became thinner and longer, as shown in Figure 6B,C. Moreover, the larvae of the negative control group had evenly distributed bristles, whereas the compound-treated groups had no continuous arrangement of bristles. According to the literature, the chemical toxicity of sophoridine derivatives may cause the deformation and hypertrophy of the intestinal epithelium of larvae, resulting in abdominal collapse. Then, the intestinal tract collapsed and noticeably darkened [46]. The first to fifth abdominal segments are in the midgut of the larva, playing a key role in the secretion of digestive enzymes [47]. Therefore, the imbalance in larval physiological function caused by the collapse of the intestinal lumen may explain the death of the larvae.

Observation of Larval Caudal Gill
The caudal gills of larvae in the control group had a clear and cyst-like structure located at the end of the larvae, as shown in Figure 7A. Noticeably, Figure 7B,C show that the normal external structure existed with a shrunken internal structure after the larvae were treated with the sophoridine derivatives. The external cuticle of the caudal gill is reportedly extensively damaged when the internal structure of the larval caudal gill shrinks [48]. Additionally, the larval caudal gill participates in the uptake and elimination of most dysfunction-related ions to regulate larval electrolyte levels. The deletion and deformation of the caudal gill could lead to significantly reduced or lost larval uptake of sodium, potassium, chloride, and phosphate ions from the nutrient matrix. Therefore, the destruction of the caudal gill caused by the sophoridine derivatives may also be one of the reasons for the death of the larvae.
The caudal gills of larvae in the control group had a clear and cyst-like structure located at the end of the larvae, as shown in Figure 7A. Noticeably, Figures 7B,C show that the normal external structure existed with a shrunken internal structure after the larvae were treated with the sophoridine derivatives. The external cuticle of the caudal gill is reportedly extensively damaged when the internal structure of the larval caudal gill shrinks [48]. Additionally, the larval caudal gill participates in the uptake and elimination of most dysfunction-related ions to regulate larval electrolyte levels. The deletion and deformation of the caudal gill could lead to significantly reduced or lost larval uptake of sodium, potassium, chloride, and phosphate ions from the nutrient matrix. Therefore, the destruction of the caudal gill caused by the sophoridine derivatives may also be one of the reasons for the death of the larvae.

Observation of Larval Tail
The tail of the larvae in the control group had a normal shape ( Figure 8A), whereas the experimental treatment groups had intestinal content discharge at the tail ( Figure  8B,C). According to the literature [47], the intestinal contents were expelled because the intestinal epithelial cells ruptured, thereby permitting the whole extrusion of the intestinal contents along with the nutritional matrix surrounding the intestine. Researchers have demonstrated that larvae underwent partial or complete elimination of nutritional substrates as a defensive strategy to remove hazardous chemicals [48,49]. The compound with a toxic effect on the larvae destroyed the intestinal epithelial cells, so the intestinal lumen completely collapsed. Moreover, the larvae started their own defense system that extruded the nutrient matrix out of the body, causing larval death. These observations suggested that the intestinal cavity, caudal gill, and tail of the larvae were all subjected to varying degrees of injury as a result of the action of sophoridine derivatives, and there is a pattern to the morphological changes in the larvae following medication treatment. Other evidence points to the role of sophoridine derivatives in the disorganization of the larvae's defense mechanism, the collapse of the intestinal lumen, and the loss of the caudal gill. These morphological alterations offer fresh

Observation of Larval Tail
The tail of the larvae in the control group had a normal shape ( Figure 8A), whereas the experimental treatment groups had intestinal content discharge at the tail ( Figure 8B,C). According to the literature [47], the intestinal contents were expelled because the intestinal epithelial cells ruptured, thereby permitting the whole extrusion of the intestinal contents along with the nutritional matrix surrounding the intestine. Researchers have demonstrated that larvae underwent partial or complete elimination of nutritional substrates as a defensive strategy to remove hazardous chemicals [48,49]. The compound with a toxic effect on the larvae destroyed the intestinal epithelial cells, so the intestinal lumen completely collapsed. Moreover, the larvae started their own defense system that extruded the nutrient matrix out of the body, causing larval death.

Observation of Larval Caudal Gill
The caudal gills of larvae in the control group had a clear and cyst-like structure located at the end of the larvae, as shown in Figure 7A. Noticeably, Figures 7B,C show that the normal external structure existed with a shrunken internal structure after the larvae were treated with the sophoridine derivatives. The external cuticle of the caudal gill is reportedly extensively damaged when the internal structure of the larval caudal gill shrinks [48]. Additionally, the larval caudal gill participates in the uptake and elimination of most dysfunction-related ions to regulate larval electrolyte levels. The deletion and deformation of the caudal gill could lead to significantly reduced or lost larval uptake of sodium, potassium, chloride, and phosphate ions from the nutrient matrix. Therefore, the destruction of the caudal gill caused by the sophoridine derivatives may also be one of the reasons for the death of the larvae.

Observation of Larval Tail
The tail of the larvae in the control group had a normal shape ( Figure 8A), whereas the experimental treatment groups had intestinal content discharge at the tail ( Figure  8B,C). According to the literature [47], the intestinal contents were expelled because the intestinal epithelial cells ruptured, thereby permitting the whole extrusion of the intestinal contents along with the nutritional matrix surrounding the intestine. Researchers have demonstrated that larvae underwent partial or complete elimination of nutritional substrates as a defensive strategy to remove hazardous chemicals [48,49]. The compound with a toxic effect on the larvae destroyed the intestinal epithelial cells, so the intestinal lumen completely collapsed. Moreover, the larvae started their own defense system that extruded the nutrient matrix out of the body, causing larval death. These observations suggested that the intestinal cavity, caudal gill, and tail of the larvae were all subjected to varying degrees of injury as a result of the action of sophoridine derivatives, and there is a pattern to the morphological changes in the larvae following medication treatment. Other evidence points to the role of sophoridine derivatives in the disorganization of the larvae's defense mechanism, the collapse of the intestinal lumen, and the loss of the caudal gill. These morphological alterations offer fresh These observations suggested that the intestinal cavity, caudal gill, and tail of the larvae were all subjected to varying degrees of injury as a result of the action of sophoridine derivatives, and there is a pattern to the morphological changes in the larvae following medication treatment. Other evidence points to the role of sophoridine derivatives in the disorganization of the larvae's defense mechanism, the collapse of the intestinal lumen, and the loss of the caudal gill. These morphological alterations offer fresh avenues for further research into the mode and mechanism of sophoridine and its derivatives' ability to harm larvae.

Conclusions
This study aimed to identify the lead compounds for anti-mosquito activity from structurally modified derivatives of the natural product sophoridine as alternatives to other synthetic insecticides currently on the market. Twenty-seven sophoridine derivatives were synthesized, and three derivatives (SOP-2g, SOP-2q, and SOP-2r) with good larvicidal activity were identified in anti-mosquito tests with LC 50 values of 330.98, 430.53, and 411.09 ppm, respectively. Such activities were lower than commercially available decpermethrin and cypermethrin. However, it was first time studying the antimosquito activity of the natural product sophoridine against Ae. albopictus, and it is hoped that the negative effects of chemical pesticides, such as potential health risks, water pollution, environmental contamination, and toxicity to non-target organisms, will be addressed in further study. SAR relationship analysis of these novel sophoridine derivatives revealed that the introduction of the oxime ester fragment led to improved biological activity against Ae. albopictus. Aliphatic long carbon chains and fused-ring fragments were introduced at the hydroxyl group of the oxime ester by esterification, respectively, which improved the larvicidal activity. The larval emergence experiment of Ae. albopictus against these derivatives showed that larval emergence was delayed by 1-2 days, and the fledging rates decreased by 7-15%. The insecticidal mechanism for larvae revealed that the inhibition rates of AChE of the three sophoridine derivatives (SOP-2g, SOP-2q, and SOP-2r) at the highest concentration of 250 ppm were 63.16%, 46.67%, and 35.11%, respectively, and SOP-2g retained a 44.72% inhibition rate even at the lowest concentration of 50 ppm. Morphological observation of the dead larvae treated with SOP-2q and SOP-2r showed that the internal structure of the caudal gills was wrinkled and the intestinal cavity and segments obviously collapsed. The abdomen was also thinner and longer with the contents squeezed out due to the deformation and extrusion of the intestinal tract. In summary, this work offered a substantive foundation for identifying efficient larvicidal agents from natural products. We provided a basis for the onward development of sophoridine acting as a natural alternative to control mosquito populations.