The Adult and Larva of a New Species of the Genus Dila (Coleoptera, Blaptinae, Blaptini) from Himalayas, with Molecular Phylogenetic Inferences of Related Genera of the Blaptini †
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Morphological Examination
2.2. Taxon Sampling, DNA Extraction, PCR Amplification, and Sequencing
2.3. Phylogenetic Analyses
3. Results
3.1. Morphological Study and Diagnosis
3.1.1. Key to Species of the Genus Dila Fischer von Waldheim, 1844, from China (Based on Males)
- 1.
- The body is slender (Figure 2A) and elongated; the parameres are finger-shaped… 2
- -
- The body is robust and weakly elongated; the parameres are cone-shaped … … … 3
- 2.
- The pronotum is wider than it is long and almost cordiform, with one tooth on theventral margin of the profemur … … … … … … … … D. laevicollis Gebler, 1841
- -
- The pronotum length and width are nearly equal, and the lateral margins show slightarcuate narrowing to the anterior margin, with obtuse-angled prominence of theprofemur … … … … … … … … … … … … … … D. ngaria Li and Ren, sp. n.
- 3.
- The pronotum is wider than it is long and almost cordiform; with two teeth on theventral margin of the profemur, without one tooth on the ventral margin of themesofemur … … … … … … … … … … … … … D. bomina Ren and Li, 2001
- -
- The pronotum length and width are nearly equal, the lateral margins showslight arcuate narrowing to the anterior margin, with one tooth on the ventralmargin of the profemur and without a tooth on the ventral margin of themesofemur … … … … … … … … … … … … … D. rugelytra Ren, 2016
3.1.2. Larva
3.2. Phylogenetic Relationships
3.3. Bionomics
4. Discussion
4.1. Taxonomic Remarks
4.2. Phylogeny and Classification of Species
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Primer (Forward/Reverse) | Sequence (Forward and Reverse) 5′ → 3′ | PCR Conditions (Annealing) | References |
---|---|---|---|---|
COI | F 2183 | CAACATTTATTTTGATTTTTTGG | 50 °C | Monteiro & Pierce, 2001 [13] |
R 3014 | TCCAATGCACTAATCTGCCATATTA | |||
Cytb | F revcb2h | TGAGGACAAATATCATTTTGAGGW | 50 °C | Simmons et al., 2001 [14] |
R rebcbj | TCAGGTCGAGCTCCAATTCATGT | |||
16S | F 13398 | CGCCTGTTTATCAAAAACAT | 50 °C | Simon et al., 1994 [15] |
R 12887 | CCGGTCTGAACTCAGATCAT | |||
28S-D2 | F 3665 | AGAGAGAGTTCAAGAGTACGTG | 58 °C | Belshaw et al., 1997 [16] |
R 4068 | TTGGTCCGTGTTTCAAGACGGG |
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Li, X.-M.; Ji, B.; Tian, J.; Ren, G.-D. The Adult and Larva of a New Species of the Genus Dila (Coleoptera, Blaptinae, Blaptini) from Himalayas, with Molecular Phylogenetic Inferences of Related Genera of the Blaptini. Insects 2023, 14, 284. https://doi.org/10.3390/insects14030284
Li X-M, Ji B, Tian J, Ren G-D. The Adult and Larva of a New Species of the Genus Dila (Coleoptera, Blaptinae, Blaptini) from Himalayas, with Molecular Phylogenetic Inferences of Related Genera of the Blaptini. Insects. 2023; 14(3):284. https://doi.org/10.3390/insects14030284
Chicago/Turabian StyleLi, Xiu-Min, Baoyue Ji, Juan Tian, and Guo-Dong Ren. 2023. "The Adult and Larva of a New Species of the Genus Dila (Coleoptera, Blaptinae, Blaptini) from Himalayas, with Molecular Phylogenetic Inferences of Related Genera of the Blaptini" Insects 14, no. 3: 284. https://doi.org/10.3390/insects14030284