The Role of Cytochrome P450 4C1 and Carbonic Anhydrase 3 in Response to Temperature Stress in Bemisia tabaci
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Insects
2.2. RNA Extraction and cDNA Synthesis
2.3. Sequence Analysis
2.4. Quantitative Real-Time PCR Analysis of Relative Expression Levels
2.5. RNA Interference and Phenotype Observation
2.6. Statistical Analysis
3. Results
3.1. Sequence and Characterization of BtCYP 4C1
3.2. Sequence and Characterization of BtCar3
3.3. mRNA Expression in B. tabaci after dsRNA Feeding
3.4. Effects of dsBtCYP 4C1-Feeding on Temperature Resistance
3.5. Effects of dsBtCar3-Feeding on Temperature Resistance
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Primer Name | Sequence Information (5′-3′) |
---|---|---|
BtCYP 4C1 | BtCYP 4C1-F | GGAGGAAGTGACAGACC |
BtCYP 4C1-R | GTAAAAACGAATACGGG | |
BtCar3 | BtCar3-F | GCGATGCGGTAGAGTTG |
BtCar3-R | CGAAAGGTTTGCTGAAGA | |
EF1-α | EF1-α-F | TAGCCTTGTGCCAATTTCCG |
EF1-α-R | CCTTCAGCATTACCGTCC | |
BtCYP 4C1 | T7 + BtCYP 4C1-F | TAATACGACTCACTATAGGGTGGATAGGCGAGGGTCT |
T7 + BtCYP 4C1-R | TAATACGACTCACTATAGGGCGAATATTGCGTTGAGC | |
BtCar3 | T7 + BtCar3-F | TAATACGACTCACTATAGGGGCCCCCTAAGGATTTATA |
T7 + BtCar3-R | TAATACGACTCACTATAGGGCTTCCGTTGTTGTCTGTCT |
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Shen, X.; Liu, W.; Wan, F.; Lv, Z.; Guo, J. The Role of Cytochrome P450 4C1 and Carbonic Anhydrase 3 in Response to Temperature Stress in Bemisia tabaci. Insects 2021, 12, 1071. https://doi.org/10.3390/insects12121071
Shen X, Liu W, Wan F, Lv Z, Guo J. The Role of Cytochrome P450 4C1 and Carbonic Anhydrase 3 in Response to Temperature Stress in Bemisia tabaci. Insects. 2021; 12(12):1071. https://doi.org/10.3390/insects12121071
Chicago/Turabian StyleShen, Xiaona, Wanxue Liu, Fanghao Wan, Zhichuang Lv, and Jianying Guo. 2021. "The Role of Cytochrome P450 4C1 and Carbonic Anhydrase 3 in Response to Temperature Stress in Bemisia tabaci" Insects 12, no. 12: 1071. https://doi.org/10.3390/insects12121071