In Vitro Protective Effect of Paste and Sauce Extract Made with Protaetia brevitarsis Larvae on HepG2 Cells Damaged by Ethanol

We made paste and sauce using protein-rich Protaetia brevitarsis larvae (PBL) and evaluated their fermentation levels. After pretreatment with the paste and sauce extracts, HepG2 cells were damaged with ethanol (EtOH), and then the effects of the paste and sauce were evaluated. As a result, we confirmed that the PBL paste and sauce extracts reduced the aspartate aminotransferase (AST) and alanine aminotransaminase (ALT) content in the medium as compared to soybean (Glycine max) sauce and paste extracts. In addition, the PBL paste and sauce extracts significantly lowered the level of tumor necrosis factor (TNF)-α and interleukin (IL)-6, which are biomarkers of inflammation, and significantly increased the inhibition rate of superoxide dismutase (SOD) and reduced glutathione (GSH), which are antioxidative indicators, in proportion to the amount of PBL added to the paste and sauce. These results suggest that an intake of PBL paste and sauce, a novel type of fermented food made from insects, may be effective for liver protection through anti-inflammatory and antioxidative effects against hepatocyte injury caused by EtOH.


Introduction
Insects are emerging as a new protein-rich food source because of the many advantages they possess as a protein food [1]. They are abundant and diverse enough to make up more than 70% of the animals on the planet, have excellent feed efficacy, are eco-friendly because they hardly emit greenhouse gases during breeding, and do not require high skill standards in breeding [2]. Asian countries have used insects as food for a long time. In particular, there are records to show that insects have been used in China as a food source since approximately 3200 years ago [3]. In Korea, Protaetia brevitarsis larvae (PBL), Tenebrio molitor larvae (TML), Allomyrina dichotoma larvae, Zophobas morio larvae, Gryllis bimaculatus, Bombyx mori larvae and pupae, and Oxya chinensis sinuosa can now be legally used as edible insects. Accordingly, studies have been actively conducted in Korea to develop various types of food using insects.
P. brevitarsis is an insect belonging to the Coleoptera Scarabaeidae family. It has already been proven as non-toxic through good laboratory practice (GLP) procedures [4,5] and reported to consist of, among others, 58% protein, 10% carbohydrate, and 16% fat [6]. PBL is known to contain useful bioactive substances, such as protacin-a natural antibiotic protein. It has also been reported that ALT, bilirubin, and bile acid levels are restored to normal levels when orally administering PBL to carbon-tetrachloride-induced rats [7]. P. brevitarsis has been used for a long time in Korea as a traditional

Preparation of Koji, Paste, and Sauce Extracts
The koji prepared in this study was milled and diluted with distilled water at a ratio of 1:10 (w/v). The diluted samples were mixed for 1 h using a shaker, centrifuged at 4000 rpm for 10 min, and then filtered using Whatman ® No.1 filter paper for primary filtration and a 0.45 µm syringe filter for secondary filtration.
The paste made from koji was freeze-dried, milled, diluted with 95% EtOH at a ratio of 1:5 (w/v), and extracted over 24 h. After centrifugation at 3000 rpm for 10 min, the supernatant was filtered using Whatman ® No.1 filter paper for primary filtration and a 0.45 µm syringe filter for secondary filtration. Afterward, the supernatant was concentrated using a speed vacuum (EYELA, Japan) for 48 h and dissolved in 20% DMSO. The sauce was centrifuged at 3000 rpm for 10 min and filtered using Whatman ® No.1 filter paper for primary filtration and a 0.45 µm syringe filter for secondary filtration. Each koji, paste, and sauce extract was applied to all the experiments in this study.

Amino Nitrogen, Protease Activity, and Free Amino Acid Analyses
A 95% EtOH extract of koji, paste, and sauce was used in the amino nitrogen and protease activity assays. Amino nitrogen was determined using a Primary Amino Nitrogen Kit (Megazyme, Chicago, IL, USA), and protease activity was determined using a Pierce™ Protease Assay Kit (Thermofisher, Tewksbury, MA, USA), according to each supplier's protocol. The extracted samples were analyzed for free amino acids, according to the method of the Korean Food Standard Codex (Ministry of Food and Drug Safety, 2019). The prepared samples were blended with 16% trichloroacetate and rocked for 15 min. After centrifugation at 3000 rpm for 15 min, the analysis of free amino acids by liquid chromatography (Agilent, Santa Clara, CA, USA) was performed with the supernatant.

Aspartate Aminotransferase (AST) and Alanine Aminotransaminase (ALT) Analyses
The media isolated in Section 2.4 were used for AST and ALT analyses. The levels of AST and ALT were measured using Asan GOT (Glutamic oxalacetic transaminase) and GPT (Glutamic pyruvate transaminase) (ASAN Pharmaceutical, Hwaseong, Korea), respectively, according to the supplier's manuals. Silymarin was used as a positive control. Silymarin has been reported to exert an antioxidant effect by removing oxygen free radicals and reducing lipid peroxidation, thereby promoting hepatocyte regeneration [24][25][26]. The AST and ALT levels are expressed in IU/L.

Glutathione (GSH) Activity and Superoxide Dismutase (SOD) Inhibition Rate Analyses
The cells isolated in Section 2.4 were lysed with a radioimmunoprecipitation assay (RIPA) buffer (Biosesang, Korea) and used for the GSH activity and SOD inhibition rate analyses. The GSH activity was analyzed using a Glutathione Assay Kit (Sigma-Aldrich, St. Louis, MO, USA), and the SOD activity (inhibition rate (%)) was analyzed using a SOD Assay Kit (Sigma-Aldrich), according to the manufacturer's protocols. Silymarin was used as a positive control. The GSH activity and the SOD inhibition rate are represented as relative values of the samples for the non-treatment control.

Statistical Analysis
The results are indicated as the mean ± standard deviation (SD), and all measurements were repeated three times. Differences between groups were tested with the Student's t-test. SPSS version 18 (SPSS Inc., Chicago, IL, USA) was used for analysis. Statistical significance was defined at 0.05 probability.

Evaluating the Fermentation of Koji Made with PBL
Amino nitrogen content, protease activity, and free amino acid content were measured to evaluate the degree of PBL fermentation by the inoculation amount of A. oryzae and fermentation time. The amino nitrogen content and protease activity increased in proportion to the quantity of A. oryzae inoculum and fermentation time. The greatest value of 0.64 mg/mL was measured when the PBL koji had been fermented for 72 h after inoculation with 0.4% A. oryzae ( Figure 1A). For the protease activity, the maximum value of 178.5% compared with the control (non-fermented PBL) was also observed when the PBL koji had been fermented for 72 h after inoculation with 0.8% A. oryzae ( Figure 1A). As a result of the free amino acid content analysis before and after fermentation, all the free amino acids except arginine and tryptophan increased after fermentation in this study ( Figure 1B). Therefore, PBL pastes and sauces were made using the PBL koji fermented with 0.8% A. oryzae for 72 h. Using this fermentation condition, PBL pastes and sauces using koji were made with different proportions of PBL and soybean: GMP100 (100% GM paste), PBP100 (100% PBL paste), PBP50 (50% PBL/50% GM), and PBP30 (30% PBL/70% GM) as pastes and GMS100 (100% GM sauce), PBS100 (100% PBL sauce), PBS50 (50% PBL/50% GM), and PBS30 (30% PBL/70% GM) as sauces.

Evaluation of Aging on Paste and Sauce Made with PBL Koji
To evaluate the degree of aging in the PBL pastes and sauces made with PBL koji, the amino nitrogen and protease activity were measured in each PBL paste and sauce ( Table 1). The amino nitrogen content and protease activity of the paste increased in proportion to the PBL concentration in the koji. For the paste, the maximum values of amino nitrogen (0.45 mg/mL) and protease activity (129.10%) were shown in PBP100. In addition, for the sauce, the amino nitrogen content and protease activity increased with the PBL in a concentration-dependent manner. The maximum values of amino nitrogen (3.88 mg/mL) and protease activity (133.53%) were measured in PBS100. Accordingly, it was found that the PBL pastes and sauces were effectively aged in proportion to the PBL content of the koji.

Evaluation of Aging on Paste and Sauce Made with PBL Koji
To evaluate the degree of aging in the PBL pastes and sauces made with PBL koji, the amino nitrogen and protease activity were measured in each PBL paste and sauce (Table 1). The amino nitrogen content and protease activity of the paste increased in proportion to the PBL concentration in the koji. For the paste, the maximum values of amino nitrogen (0.45 mg/mL) and protease activity (129.10%) were shown in PBP100. In addition, for the sauce, the amino nitrogen content and protease activity increased with the PBL in a concentration-dependent manner. The maximum values of amino nitrogen (3.88 mg/mL) and protease activity (133.53%) were measured in PBS100. Accordingly, it was found that the PBL pastes and sauces were effectively aged in proportion to the PBL content of the koji. Table 1. Amino nitrogen level and relative protease activity of each paste and sauce to evaluate PBL koji paste and sauce fermentation. The amino nitrogen level of each paste and sauce was analyzed. In addition, the protease activity of each paste and sauce was evaluated.

EtOH Concentration Causing Toxicity to Hepatocytes and Toxicity Assessment of PBL Paste and Sauce to Hepatocytes
In order to determine the concentration of EtOH that can damage HepG2 cells, the cell viability was measured after adding EtOH at different concentrations. As a result, the viabilities of the HepG2 cells treated with 100, 200, 300, 400, and 500 mM EtOH were 88%, 85%, 67%, 57%, and 33%, respectively. Since a significant difference of p < 0.001 was shown in the 300 mM EtOH concentration, 300 mM was determined as the concentration of EtOH that causes toxicity to hepatocytes ( Figure 2A).
In order to evaluate the toxicity of the paste and sauce, the extracts were treated with different concentrations in HepG2 cells. For the paste extract, cell viability decreased at 500 µg/mL of GMP100, and for the sauce extract, cell viability decreased at 50 µl/mL of GMS100 ( Figure 2B,C). Accordingly, it was found that the maximum concentrations that did not cause toxicity to the cells of the pastes and sauces used in this study were 400 µg/mL and 40 µl/mL, respectively.
To evaluate the effect of the PBL paste and sauce on hepatocytes damaged by EtOH, the HepG2 cells were treated with the determined concentration of the PBL paste and sauce extracts ( Figure 2D). After treating with 300 mM EtOH, the cells were incubated for 24 h, and then the cell viability was examined. The cell viability of the group treated with only 300 mM EtOH, without the paste or sauce extract, was 75.13%. The viability of cells pretreated with silymarin (positive control), GMP100, PBP30, PBP50, PBP100, PBS30, PBS50, and PBS100 were 98.43%, 96.84%, 95.68%, 94.99%, 96.18%, 94.85%, and 94.57%, respectively. Considering the above results, it can be stated that all the PBL pastes and sauces used in this study effectively protect HepG2 cells against alcohol damage. (B,C) show the cell viability after 300 mM EtOH treatment in the HepG2 cells pretreated with the paste and sauce extracts, respectively, by concentration. Values are the means ± standard deviations of triplicate experiments. * p < 0.05 indicates a significant difference between the control and 500 μg/mL of the GMP100 treatment. (D) Cell viability after 300 mM EtOH treatment in the HepG2 cells pretreated with the paste and sauce extracts. Values are the means ± standard deviations of triplicate experiments. ## p < 0.01 indicates a significant difference between the EtOH (-)/treatment (-) and the EtOH (+)/treatment (-). * p < 0.05 and ** p < 0.01, *** p < 0.001 indicate a significant difference between the EtOH (+)/treatment (-) and the EtOH (+)/treatment (+). S, silymarin (100 μg/mL); GMP100, paste made from 100% GM; PBP30, paste made from 30% PBL/70% GM; PBP50, paste made from 50% PBL/50% GM; PBP100, paste made from 100% PBL; GMS100, sauce made from 100% GM; PBS30, sauce made from 30% PBL/70% GM; PBS50, sauce made from 50% PBL/50% GM; PBS100, sauce made from 100% PBL. The concentrations of paste and sauce were 400 μg/mL and 40 μl/mL, respectively. Values are the means ± standard deviations of triplicate experiments.

Hepatoprotective Effect of Pretreatment with PBL Paste and Sauce on EtOH-Damaged Hepatocytes
The AST and ALT levels were measured to evaluate the hepatoprotective potential of PBL paste and sauce in this study (Table 2). First, we proved that the AST and ALT levels in HepG2 cells damaged by EtOH were 56.45 IU/L and 34.46 IU/L, respectively, and that they significantly rose compared to those without EtOH treatment (23.23 IU/L and 25.67 IU/L, respectively). Then, we determined whether pretreating with PBL paste and sauce extract before the EtOH treatment significantly decreased the AST and ALT levels in HepG2 cells as occurred with the silymarin pretreatment (23.35 IU/L and 18.24 IU/L, respectively), which was the positive control.

Hepatoprotective Effect of Pretreatment with PBL Paste and Sauce on EtOH-Damaged Hepatocytes
The AST and ALT levels were measured to evaluate the hepatoprotective potential of PBL paste and sauce in this study (Table 2). First, we proved that the AST and ALT levels in HepG2 cells damaged by EtOH were 56.45 IU/L and 34.46 IU/L, respectively, and that they significantly rose compared to those without EtOH treatment (23.23 IU/L and 25.67 IU/L, respectively). Then, we determined whether pretreating with PBL paste and sauce extract before the EtOH treatment significantly decreased the AST and ALT levels in HepG2 cells as occurred with the silymarin pretreatment (23.35 IU/L and 18.24 IU/L, respectively), which was the positive control.
The AST levels in the EtOH-injured HepG2 cells after pretreatment with GMP100, PBP30, PBP50, and PBP100 significantly decreased compared to only EtOH-treated HepG2 cells (56.45 IU/L). The AST levels in the cells pretreated with GMP100, PBP30, PBP50, and PBP100 extracts were 36.11, 37.35, 29.69, and 25.26 IU/L, respectively. For the pretreatment with GMS100, PBS30, PBS50, and PBS100 extracts, the AST levels were 36.78, 35.18, 33.47, and 28.23 IU/L, respectively. Accordingly, pretreatment with GMS100, PBP30, PBP50, PBP100, PBS30, PBS50, and PBS100 extracts significantly decreased the AST levels in EtOH-damaged HepG2 cells compared to only EtOH-treated HepG2 cells. In addition, compared to the single substance, silymarin (23.32 IU/L), a similar decrease was observed for all treatments (p < 0.01). The ALT levels in the EtOH-damaged HepG2 cells after pretreatment with PBP30, 50, and 100 extracts were significantly decreased compared with only EtOH-treated HepG2 cells (34.46 IU/L). The ALT levels in the cells pretreated with GMP100, PBP30, PBP50, and PBP100 extracts were 24.59, 27.62, 21.27, and 19.75 IU/L, respectively. Likewise, for the pretreatment with GMS100, PBS30, PBS50, and PBS100 extracts, the ALT levels were 26.43, 25.66, 20.55, and 19.22 IU/L, respectively. These results showed that the AST and ALT levels in the EtOH-injured HepG2 cells after pretreatment with PBL paste and sauce were lower than those after treatment with GM paste and sauce, except PBP30. The ALT levels showed a decrease similar to that of silymarin (18.24 IU/L) when the larvae contents were 50% and 100%. As the content of insects increased in the paste and sauce, it was found that the AST and ALT levels decreased. In particular, it was confirmed that the reduction effect was highest in the paste and sauce made with 100% PBL. Although the crude extract of the paste and sauce was used, the hepatoprotective activity was similar to that of silymarin, a single substance. In addition, when the content of insects was more than 50%, the AST and ALT reduction effects were greater than those of the GM-based paste and sauce. S, silymarin (100 µg/mL); GMP100, paste made from 100% GM; PBP30, paste made from 30% PBL/70% GM; PBP50, paste made from 50% PBL/50% GM; PBP100, paste made from 100% PBL; GMS100, sauce made from 100% GM; PBS30, sauce made from 30% PBL/70% GM; PBS50, sauce from 50% PBL/50% GM; PBS100, sauce made from 100% PBL. The concentrations of the paste and sauce were 400 µg/mL and 40 µl/mL, respectively. Values are the means ± standard deviations of triplicate experiments. ## p < 0.01 indicates a significant difference between the EtOH (−)/treatment (−) and the EtOH (+)/treatment (−). ** p < 0.01 and *** p < 0.001 indicate a significant difference between the EtOH (+)/treatment (−) and the EtOH (+)/treatment (+).

Evaluation of Anti-Inflammatory Potential of PBL Paste and Sauce in EtOH-Damaged Hepatocytes
To investigate the anti-inflammatory potential of PBL paste and sauce on EtOH-damaged hepatocytes, the expression levels of TNF-α and IL-6 mRNA were measured in HepG2 cells treated with 300 mM EtOH after pretreatment with PBL paste and sauce extract (Figure 3). First, it was proven that the expression levels of TNF-α and IL-6 transcripts, which are biomarkers of inflammation, were significantly increased in EtOH-treated HepG2 cells compared to normal HepG2 cells (EtOH (−)/treatment (−)). The expression levels of TNF-α in the EtOH-injured HepG2 cells after pretreatment with silymarin, GMP100, PBP30, PBP50, and PBP100 extracts were 0.63-, 0.92-, 0.74-, 0.77-, and 0.65-fold, respectively, compared to the EtOH-treatment control (4.74-fold) ( Figure 3A). On the other hand, for the pretreatment with silymarin, GMS100, PBS30, PBS50, and PBS100 extracts, the expression levels of TNF-α were 0.55-, 2.99-, 0.69-, 1.01-, and 0.72-fold, respectively, compared to the EtOH-treatment control (6.64-fold) ( Figure 3B). The expression levels of IL-6 in the EtOH-injured HepG2 cells after silymarin, GMP100, PBP30, PBP50, and PBP100 treatment were 0.59-, 0.82-, 0.83-, 0.87-, and 0.79-fold, respectively, compared to the EtOH-treatment control (2.85-fold) ( Figure 3C). Likewise, for the pretreatment with silymarin, GMS100, PBS30, PBS50, and PBS100, the expression levels of IL-6 were 0.64-, 1.03-, 0.87-, 0.80-, and 0.54-fold, respectively, compared to the EtOH-treatment control (2.77-fold) ( Figure 3D). Based on the above results, the expression levels of TNF-α and IL-6 decreased similarly to those of silymarin at p < 0.001. In addition, the lowest expressions of the two transcripts in the PBL paste and sauce extracts were observed when the insect content was 100%. For the anti-inflammatory effect, treating with the paste and sauce made of PBL results in a higher suppression of TNF-α expression in EtOH-damaged hepatocytes than treating with the paste and sauce made of GM.
Insects 2020, 11, x FOR PEER REVIEW 9 of 13 levels of IL-6 were 0.64-, 1.03-, 0.87-, 0.80-, and 0.54-fold, respectively, compared to the EtOHtreatment control (2.77-fold) ( Figure 3D). Based on the above results, the expression levels of TNF-α and IL-6 decreased similarly to those of silymarin at p < 0.001. In addition, the lowest expressions of the two transcripts in the PBL paste and sauce extracts were observed when the insect content was 100%. For the anti-inflammatory effect, treating with the paste and sauce made of PBL results in a higher suppression of TNF-α expression in EtOH-damaged hepatocytes than treating with the paste and sauce made of GM. Figure 3. Anti-inflammatory potential of the paste and sauce made from PBL koji. TNF-α levels were analyzed after 300 mM EtOH treatment on HepG2 cells pretreated with 95% EtOH extract of each paste (A) and sauce (B). IL-6 levels were measured after 300 mM EtOH treatment on HepG2 cells pretreated with 95% EtOH extract of each paste (C) and sauce (D). S, silymarin (100 μg/mL); GMP100, paste made from 100% GM; PBP30, paste made from 30% PBL/70% GM; PBP50, paste made from 50% PBL/50% GM; PBP100, paste made from 100% PBL; GMS100, sauce made from 100% GM; PBS30, sauce made from 30% PBL/70% GM; PBS50, sauce made from 50% PBL/50% GM; PBS100, sauce made from 100% PBL. The concentrations of the paste and sauce were 400 μg/mL and 40 μl/mL, respectively. Values are the means ± standard deviations of triplicate experiments. ### p < 0.001 indicates a significant difference between the EtOH (-)/treatment (-) and the EtOH (+)/treatment (-). *** p < 0.001 indicates a significant difference between the EtOH (+)/treatment (-) and the EtOH (+)/treatment (+).

Evaluation of Antioxidant Potential of PBL Paste and Sauce in EtOH-Damaged Hepatocytes
To examine the antioxidant potential of the PBL paste and sauce, we measured the GSH activity and SOD activity ( Table 3). The GSH activity and SOD inhibition rates were 68.70% and 87.78%, respectively, in the EtOH-treated HepG2 cells as compared to the non-treated control. The GSH activity rates in the EtOH-injured HepG2 cells after pretreatment with PBP30, 50, and 100 extracts were significantly increased compared with those treated only with EtOH (68.70%). The relative GSH Figure 3. Anti-inflammatory potential of the paste and sauce made from PBL koji. TNF-α levels were analyzed after 300 mM EtOH treatment on HepG2 cells pretreated with 95% EtOH extract of each paste (A) and sauce (B). IL-6 levels were measured after 300 mM EtOH treatment on HepG2 cells pretreated with 95% EtOH extract of each paste (C) and sauce (D). S, silymarin (100 µg/mL); GMP100, paste made from 100% GM; PBP30, paste made from 30% PBL/70% GM; PBP50, paste made from 50% PBL/50% GM; PBP100, paste made from 100% PBL; GMS100, sauce made from 100% GM; PBS30, sauce made from 30% PBL/70% GM; PBS50, sauce made from 50% PBL/50% GM; PBS100, sauce made from 100% PBL. The concentrations of the paste and sauce were 400 µg/mL and 40 µl/mL, respectively. Values are the means ± standard deviations of triplicate experiments. ### p < 0.001 indicates a significant difference between the EtOH (−)/treatment (−) and the EtOH (+)/treatment (−). *** p < 0.001 indicates a significant difference between the EtOH (+)/treatment (−) and the EtOH (+)/treatment (+).

Discussion
In this study, fermented foods (PBL paste and sauce) were prepared using PBL larvae composed of approximately 58% protein on a dry weight basis. The hepatocyte protective effects of PBL paste and sauce, which have long been reported to have liver protection efficacy, against ethanol damage were investigated [28].
As a result of the analysis of 16 free amino acids in koji after fermentation, all the free amino acids except arginine and tryptophan significantly increased. However, according to previous reports, during the fermentation process of GM, only glutamic acid, glycine, aspartic acid, and proline increase rapidly [29]. Therefore, the intake of PBL fermented foods has the advantage of supplying various amino acids that are insufficient in GM fermented foods. Meanwhile, the degree of fermentation in PBL paste and sauce increased in proportion to the content of PBL, which is presumed to be due to the higher content of protein in PBL (58%) than in GM (40%) [6,29].
AST and ALT hepatic enzymes have been commonly used as indices to determine hepatocyte injury severity, because they tend to increase in the blood amid necrosis and tissue destruction of hepatocytes during hepatocellular injury [30][31][32][33]. The AST and ALT levels were significantly reduced in the EtOH-injured hepatocytes pretreated with the PBL paste and sauce extracts, proportionately with the amount of PBL content. The AST and ALT values in this study were not significantly different from the levels of AST and ALT in hepatocytes damaged by EtOH pretreated with extracts of TML paste and sauce containing 30% ( 19.67 IU/L) of TML, which has previously been reported to have hepatoprotective efficacy in rats [6,33]. In addition, the reduction effect of these AST and ALT levels was superior to the traditional GMP100 and GMS100. Therefore, our results show that a daily intake of PBL paste and sauce may have a defensive effect against hepatocyte injury by EtOH.
In EtOH-injured hepatocytes pretreated with the PBL paste and sauce extracts, the expression grades of TNF-α and IL-6 were inhibited at similar levels to those of the pretreatment with silymarin, which has been known to have hepatoprotective, anti-inflammatory, antioxidant, and antiapoptosis effects [34][35][36][37]. Besides, except for the IL-6 level of PBP, it was assured that the PBL pastes and sauces showed a better TNF-α and IL-6 expression inhibitory effect than paste and sauce made by GM. Accordingly, we expect the PBL paste and sauce to have an outstanding anti-inflammatory effect on EtOH-injured hepatocytes. In addition, the inhibition of TNF-α and IL-6 expression in this study by pretreating with PBL paste and sauce extracts was considered a similar anti-inflammatory effect to that of the pretreatment with 30% (TMP30: 0.76-fold, 0.75-fold; TMS30: 0.65-fold, 0.88-fold), 50% (TMP50: 0.73-fold, 0.59-fold; TMS50: 0.84-fold, 0.67-fold), and 100% (TMP100: 0.67-fold, 0.78-fold; TMS100: 0.59-fold, 0.75-fold) TML paste and sauce extracts [22]. These results could be attributed to the fact that PBL, an animal protein, has a higher protein content and a different amino acid composition than GM, a plant protein, and has a higher liver protective effect and anti-inflammatory effect. In addition, our findings are consistent with the results that the expression levels of IL-6, TNF-α, and TGF-β decreased when orally administering P. brevitarsis larvae extract to rats with liver damage induced by CCl 4 [33,38,39].
The above results suggest that PBL fermented food will protect the liver by inhibiting inflammation through the suppression of cytokine expression, raising GSH activity and the SOD inhibition rate, which are biomarkers of an antioxidant effect. In addition, this activity is similar to that of silymarin, used as a positive control. In the future, it will be necessary to separate a single active substance with hepatoprotective activity from PBL paste and sauce to investigate the mechanisms for hepatoprotective effects.

Conclusions
As a result of evaluating the efficacy of PBL after applying alcohol damage to hepatocytes pretreated with PBL paste and sauce extracts, it was found that PBL paste and sauce protects hepatocytes through antioxidant and anti-inflammatory effects. The degree of liver protection efficacy of the PBL paste and sauce was generally better than that of GM, similar to silymarin, and proportional to the amount of insect content included in the paste and sauce. 'Therefore, we suggest that PBL fermented food is a new food that not only has excellent taste and nutrition but also has the potential to assist in liver protection.