Ultrasensitive Label-Free Detection of Unamplified Multidrug-Resistance Bacteria Genes with a Bimodal Waveguide Interferometric Biosensor

Infections by multidrug-resistant bacteria are becoming a major healthcare emergence with millions of reported cases every year and an increasing incidence of deaths. An advanced diagnostic platform able to directly detect and identify antimicrobial resistance in a faster way than conventional techniques could help in the adoption of early and accurate therapeutic interventions, limiting the actual negative impact on patient outcomes. With this objective, we have developed a new biosensor methodology using an ultrasensitive nanophotonic bimodal waveguide interferometer (BiMW), which allows a rapid and direct detection, without amplification, of two prevalent and clinically relevant Gram-negative antimicrobial resistance encoding sequences: the extended-spectrum betalactamase-encoding gene blaCTX-M-15 and the carbapenemase-encoding gene blaNDM-5 We demonstrate the extreme sensitivity and specificity of our biosensor methodology for the detection of both gene sequences. Our results show that the BiMW biosensor can be employed as an ultrasensitive (attomolar level) and specific diagnostic tool for rapidly (less than 30 min) identifying drug resistance. The BiMW nanobiosensor holds great promise as a powerful tool for the control and management of healthcare-associated infections by multidrug-resistant bacteria.


Introduction
Hospital-acquired or healthcare-associated infections (HAIs) refer to an infection that develops at least 48 h after the patient is admitted to a hospital [1]. These infections are caused during a prolonged stay at the hospital and are a major risk factor for serious health issues leading even to death [2]. In Europe, 3-10% of all hospitalizations in acute care hospitals (ACH) and long-term care facilities (LTCF) result in nosocomial infections, with the highest rates in surgical (7%) and intensive care units (19%) [3]. Consequently, it is estimated that a total of 8.9 million HAIs occur each year in European hospitals and LTCF. The most frequently reported microorganisms causing HAIs in Europe during 2016-2017 were Gram-negative bacteria, with Enterobacterales (38%) as the most prevalent, of which Escherichia coli (16%) and Klebsiella pneumoniae (10%) were the most frequently found, followed by other non-Enterobacterales Gram-negative bacteria (14%), with Pseudomonas aeruginosa (8%) and Acinetobacter spp (3%) as the most commonly isolated species [4].
(fundamental and first order light modes of the same polarization) travel to the end of the device [27]. By opening a window in the bimodal section, the waveguide is exposed to the external medium, where the bioreceptors are immobilized. When a biomolecular event occurs, the two light modes are affected through their evanescent tails, which induces a phase variation in the interferometric output. This interferometric technology has been already employed for other challenging clinical applications, such as for early cancer diagnosis by analyzing microRNA in urine to stratify bladder cancer patients [28] and for the analysis of mRNA alternative splicing isoforms [29], among others [30].
To demonstrate that the BiMW biosensor can be employed as a specific tool for rapidly identify MDR bacteria, we targeted two prevalent and clinically relevant Gram-negative antimicrobial resistance markers: the extended-spectrum betalactamase-encoding gene blaCTX-M-15 and the carbapenemase-encoding gene blaNDM-5 [31,32]. We have implemented a methodology using minimal pre-analytical steps such as DNA extraction, fragmentation, and denaturation, allowing the gene DNA detection with the BiMW biosensor. The high sensitivity of the BiMW nanobiosensor allows the rapid detection (≤ 40 min, including sample pretreatment) of genomic DNA (< 1000 mer) at a concentration in the range of 20-30 aM, employing a low amount of DNA sample and without the need for any DNA amplification step. We have demonstrated the capability and applicability of the BiMW biosensor for the detection of multidrug-resistance markers in E. coli, without any amplification. This can represent a valuable methodology to be used for HAIs diagnosis and implementing early and accurate therapeutic interventions.

BiMW Device and Experimental Set-Up
The working principle of the BiMW interferometric sensor has been widely explained in our previous works [26,27]. In brief, the sensor consists of a straight waveguide supporting two monochromatic light modes of the same polarization in the visible range. The sensor operates by the interference of the fundamental and first order light modes, supporting in a single mode and a bimodal region of the waveguide. The waveguide of the BiMW has a structure of Si/SiO 2 /Si 3 N 4 /SiO 2 with a width of 2.5-3 µm, a rib height of 2-3 nm, and a length of 3 cm; the thickness of the core for the single-mode and bimodal sections is 150 nm and 340 nm, respectively. A sensing window (15,000 × 50 µm) is defined in the bimodal section of the BiMW. The BiMW devices are fabricated at wafer level in clean room facilities using standard microelectronics technologies. Each BiMW sensor chip has a dimension of 10 × 30 mm and contains 20 independent and identical sensors ( Figure 1).
For the evaluation of the BiMW sensors, a bench-top set-up was employed. A He/Ne laser (λ = 632.8 nm) was used as the light source (using TE polarization). The light was end-coupled into the waveguide facet using a 40 × microscope objective. The interferometric bimodal pattern at the output of the bimodal waveguide was captured by a two-sectional Si photodiode, which was placed close enough to the sensor chip output edge for a minimum light dispersion. Each section of Diagnostics 2020, 10, 845 4 of 12 the photodiode was connected to a current amplifier in order to amplify the currents. These values were used to calculate the parameter S (signal), according to the Equation (1): where I up and I down are the currents measured by the upper and lower sections, respectively, of a two-section photodiode. ∆φ is the phase variation, which is proportional to the signal S. The intrinsic sensitivity of the BiMW sensor to temperature fluctuations was compensated by incorporating a Peltier element behind the sensor chip and a temperature controller, providing temperature stabilization with an accuracy of 0.01 • C. Data acquisition and analysis were performed using home-made LabVIEW (National Instruments, Northampton, MA, USA) software. For the evaluation of the BiMW sensors, a bench-top set-up was employed. A He/Ne laser (λ = 632.8 nm) was used as the light source (using TE polarization). The light was end-coupled into the waveguide facet using a 40 × microscope objective. The interferometric bimodal pattern at the output of the bimodal waveguide was captured by a two-sectional Si photodiode, which was placed close enough to the sensor chip output edge for a minimum light dispersion. Each section of the photodiode was connected to a current amplifier in order to amplify the currents. These values were used to calculate the parameter S (signal), according to the equation (1):

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(1) Where Iup and Idown are the currents measured by the upper and lower sections, respectively, of a two-section photodiode. Δϕ is the phase variation, which is proportional to the signal S. The intrinsic sensitivity of the BiMW sensor to temperature fluctuations was compensated by incorporating a Peltier element behind the sensor chip and a temperature controller, providing temperature stabilization with an accuracy of 0.01 °C. Data acquisition and analysis were performed using homemade LabVIEW (National Instruments, Northampton, MA, USA) software.

Microfluidic System
A microfluidic cell and a flow delivery system were implemented for the evaluation. For that, a SU-8 2025 master mold was manufactured over silicon wafer using a standard photolithography protocol in clean room facilities, which was replicated in polydimethylsiloxane (PDMS) polymer for microfluidic cell fabrication. Elastomer and curing agent were mixed in a 10:1 ratio, and air bubbles originating from the mixing were removed by a vacuum pump in a desiccator. The PDMS was cured

Microfluidic System
A microfluidic cell and a flow delivery system were implemented for the evaluation. For that, a SU-8 2025 master mold was manufactured over silicon wafer using a standard photolithography protocol in clean room facilities, which was replicated in polydimethylsiloxane (PDMS) polymer for microfluidic cell fabrication. Elastomer and curing agent were mixed in a 10:1 ratio, and air bubbles originating from the mixing were removed by a vacuum pump in a desiccator. The PDMS was cured for 1 h at 75 • C on a hotplate to ensure polymer cross-linking. The PDMS microfluidic cell has eight channels, with a separation between them of 250 µm (center to center), with an independent inlet and outlet for each channel. Each microchannel has a length of 18 mm, a height of 50 µm, and a width of 150 µm, which covers the entire sensing window of the BiMW. The microfluidic cell was connected to a syringe pump and an injection valve for fluid delivery. The flow rate was modified according to the bioassay requirements, at a range from 5 to 30 µL min −1 .

BiMW Surface Cleaning, Activation, and Biofunctionalization
The BiMW sensor surface was deeply cleaned prior to use in order to remove any contamination that can affect the subsequent silanization procedure. The BiMW sensor chips were cleaned with acetone, ethanol, and water, followed by sonication in methanol/hydrochloric acid 1:1 for 10 min, rinsing with water, and drying with a stream of nitrogen. Then, a layer of active hydroxyl group was generated by oxidation using an UV/O 3 cleaner (BioForce Nanosciences, Salt Lake City, UT, USA) for 1 h, followed by the immersion of the sensor chip in a 10% HNO 3 solution at 75 • C for 25 min. Clean and hydroxyl-activated sensor chips were immediately incubated with a silane-PEG-COOH solution of 25 mg mL −1 ethanol/water 95:5 (v/v) for 2 h at 4 • C; then, they were rinsed with ethanol and water, and dried with a nitrogen stream. The curing step was performed by placing the sensor chips into a bottle glass in an autoclave for 90 min at 120 • C and a pressure of 1.5 bars. Finally, the immobilization of the DNA probes was carried out ex situ. First, the carboxyl groups on the sensor surface were activated with a solution of 0.4 M EDC/0.1 M sulfo-NHS in MES buffer for 3 h. The amino-modified DNA probe (20 µM prepared in 10 mM PBS containing 1 mM MgCl 2 ) was incubated over the sensor surface overnight at room temperature.

Probes Design
Capture probes were designed by the free software OligoArchitectTM. This in-silico evaluation can be applied to select highly performing probes for biosensing applications. In this study, the blaCTX-M-15 and blaNDM-5 genes were the DNA target. All the probes were modified with the amine terminal group (5 -amine-probe) in order to react with the carboxyl group introduced on the surface of the sensor chip with the silane-PEG-COOH. Finally, synthetic DNA sequences were used as positive and negative controls. HPLC-purified DNA material was purchased from Ibian Technology, S.L. (Zaragoza, Spain). Detailed information about all the DNA sequences (capture probes and synthetic targets) employed in this study is provided in Table 1.  The following bacterial isolates were used: E. coli 85BA carrying blaCTX-M-15, and E. coli 1195rut carrying blaNDM-5. Additionally, P. aeruginosa and E. coli, both negative for blaCTX-M-15 and blaNDM-5 were used as negative control. All bacteria were streaked onto Luria broth (LB) and incubated overnight at 37 • C. Purification of bacteria was carried out by centrifugation at 3000 rpm for 15 min at 4 • C. Bacteria were then suspended in PBST. For bacterial DNA extraction, a DNA extraction kit (QIAamp genomic DNA kit, Hilden, Germany) was used according to the manufacturer's instructions. The extracted DNA samples were analysed by gel electrophoresis, and the concentration of the total bacterial DNA was quantified using a nanospectrophotometer at 260 nm (Nanophotometer™ from Implen, Munich, Germany). The concentrations of the 10-fold diluted extracted DNA from bacteria with the genes were 12.5 pM for the blaCTX-M-15 gene sample, and 20.91 pM for the blaNDM-5 gene sample. Regarding negative controls, the DNA concentration from the extraction step was 0.12 nM for E. coli, and 71.2 pM for P. aeruginosa, respectively. These DNA samples were extracted from a bacteria concentration around 1 × 10 7 CFU mL −1 . To validate the presence of the blaCTX-M-15 and blaNDM-5 genes, a PCR test of the samples was carried out as previously described [33][34][35][36], and evaluated in agarose electrophoretic gel from amplification with PCR.

BiMW Assay Development
Prior to the BiMW biosensor analysis, all bacteria samples were fragmented by dipping an immersion probe directly in the sample for 15 s at 120 KHz, followed by vortexing for 1 min at 3000 rpm. All double-strand-DNA-containing samples were then heated to 95 • C for 5 min to separate the two DNA strands. Strands re-hybridization was prevented by cooling the samples on ice for 1 min before their introduction into the BiMW biosensor. During all measurements, 0.5 M NaCl/TE (hybridization buffer) was flowed over the sensor surface at a constant flow rate until a stable baseline was reached. The detection of genomic DNA targets was performed by injection of 150 µL of the sample over the surface of the BiMW biosensor at 5 µL min −1 rate. In order to reuse the bioreceptor DNA layer after each measurement, an injection of HCl (100 mM) was employed to disrupt DNA-DNA hybridization at 20 µL min −1 for 30 s. Calibration curves were obtained by triplicate measurements of different concentrations of genomic DNA. The theoretical limit of detection (LOD) was determined from a linear regression fitting. The data were analyzed using Origin pro 8.0 software (Originlab, Northampton, MA, USA). Figure 2A shows the detection strategy proposed for the BiMW biosensor. Following the bacterial lysis for total DNA extraction from the specimen, a genomic DNA fragmentation was done obtaining random DNA fragments between 100 and 1000 bp, which may allow efficient hybridization with the immobilized probe. DNA denaturation of the double-stranded DNA was done using a thermal treatment. Besides, the in silico approach by OligoArchitectTM was used to design the most suitable probes sequences (18-20 mer), which target the most conserved region (1436-2311), in order to assure a high degree of specificity within the corresponding genes. Finally, the genomic DNA detection was performed by hybridization with the specific probes for each gene, previously immobilized on the BiMW sensor surface.

Bioassay Strategy and Evaluation with Synthetic DNA Target Sequences
Diagnostics 2020, 10, x FOR PEER REVIEW 6 of 13 samples were extracted from a bacteria concentration around 1 × 10 7 CFU mL −1 . To validate the presence of the blaCTX-M-15 and blaNDM-5 genes, a PCR test of the samples was carried out as previously described [33][34][35][36], and evaluated in agarose electrophoretic gel from amplification with PCR.

BiMW Assay Development
Prior to the BiMW biosensor analysis, all bacteria samples were fragmented by dipping an immersion probe directly in the sample for 15 s at 120 KHz, followed by vortexing for 1 min at 3000 rpm. All double-strand-DNA-containing samples were then heated to 95 °C for 5 min to separate the two DNA strands. Strands re-hybridization was prevented by cooling the samples on ice for 1 min before their introduction into the BiMW biosensor. During all measurements, 0.5 M NaCl/TE (hybridization buffer) was flowed over the sensor surface at a constant flow rate until a stable baseline was reached. The detection of genomic DNA targets was performed by injection of 150 µL of the sample over the surface of the BiMW biosensor at 5 µL min −1 rate. In order to reuse the bioreceptor DNA layer after each measurement, an injection of HCl (100 mM) was employed to disrupt DNA-DNA hybridization at 20 µL min −1 for 30 s. Calibration curves were obtained by triplicate measurements of different concentrations of genomic DNA. The theoretical limit of detection (LOD) was determined from a linear regression fitting. The data were analyzed using Origin pro 8.0 software (Originlab, Northampton, MA, USA). Figure 2A shows the detection strategy proposed for the BiMW biosensor. Following the bacterial lysis for total DNA extraction from the specimen, a genomic DNA fragmentation was done obtaining random DNA fragments between 100 and 1000 bp, which may allow efficient hybridization with the immobilized probe. DNA denaturation of the double-stranded DNA was done using a thermal treatment. Besides, the in silico approach by OligoArchitectTM was used to design the most suitable probes sequences (18-20 mer), which target the most conserved region (1436-2311), in order to assure a high degree of specificity within the corresponding genes. Finally, the genomic DNA detection was performed by hybridization with the specific probes for each gene, previously immobilized on the BiMW sensor surface. For a proper oligonucleotide immobilization, the silicon nitride (Si 3 N 4 ) surface of the BiMW sensor was chemically modified with silane-PEG-COOH in order to generate a homogenous monolayer with anti-fouling properties and exposing reactive carboxyl groups [37]. For the activation of the silane-PEG-COOH monolayer, we employed the well-known EDC/NHS surface chemistry. Figure 2B shows a scheme of the biofuctionalization process.

Bioassay Strategy and Evaluation with Synthetic DNA Target Sequences
To demonstrate the capability of the BiMW biosensor for genomic DNA detection, we first evaluated the detection of a 88 mer synthetic DNA target for blaCTX-M-15 gene using the capture probe (CTX-M-15 F probe, see Table 1). The probe was previously immobilized onto the BiMW sensor surface, and the corresponding hybridization was monitored at different target concentrations (0.1, 1, and 10 nM) (see Figure 3A). The specificity was also assessed by monitoring the biosensor response after injection of a synthetic negative control at 10 nM concentration. As can be observed in Figure 3B, the negative control sequence recovers the baseline after the running buffer passes over the biosensor surface, while the blaCTX-M-15 synthetic DNA target produces a clearly noticeable signal, confirming the specific detection of the sequence blaCTX-M-15. The phase variation for the blaCTX-M-15 synthetic DNA target (at 10 nM) was 0.08 × 2πrad, while for the synthetic negative control (at 10 nM) was almost negligible, 1.3 × 10 −3 × 2πrad. Figure 3C shows the average signal obtained for the specific blaCTX-M-15 synthetic DNA target and for the negative controls. For a proper oligonucleotide immobilization, the silicon nitride (Si3N4) surface of the BiMW sensor was chemically modified with silane-PEG-COOH in order to generate a homogenous monolayer with anti-fouling properties and exposing reactive carboxyl groups [37]. For the activation of the silane-PEG-COOH monolayer, we employed the well-known EDC/NHS surface chemistry. Figure 2B shows a scheme of the biofuctionalization process.
To demonstrate the capability of the BiMW biosensor for genomic DNA detection, we first evaluated the detection of a 88 mer synthetic DNA target for blaCTX-M-15 gene using the capture probe (CTX-M-15 F probe, see Table 1). The probe was previously immobilized onto the BiMW sensor surface, and the corresponding hybridization was monitored at different target concentrations (0.1, 1, and 10 nM) (see Figure 3A). The specificity was also assessed by monitoring the biosensor response after injection of a synthetic negative control at 10 nM concentration. As can be observed in Figure  3B, the negative control sequence recovers the baseline after the running buffer passes over the biosensor surface, while the blaCTX-M-15 synthetic DNA target produces a clearly noticeable signal, confirming the specific detection of the sequence blaCTX-M-15. The phase variation for the blaCTX-M-15 synthetic DNA target (at 10 nM) was 0.08 × 2πrad, while for the synthetic negative control (at 10 nM) was almost negligible, 1.3 × 10 −3 × 2πrad. Figure 3C shows the average signal obtained for the specific blaCTX-M-15 synthetic DNA target and for the negative controls.

Betalactamase CTX-M-15
In order to demonstrate the biosensor capability for the analysis of real samples, the next step was the evaluation of real bacterial culture samples. Two sequences (forward and reverse), specific for the blaCTX-M-15 gene, were selected as probes and immobilized on the sensor surface (see Figure 4A). The use of forward and reverse probes was intended for increasing the possibility to detect both DNA strands, and to increase the sensitivity of the biosensor. from P. aeruginosa at a concentration of 3.4 fM and from E. coli lacking blaCTX-M-15 gene at a concentration of 2.9 fM. These concentrations of the negative controls are similar to the highest concentration of the blaCTX-M-15 target used. These signals show a phase variation of 0.25 × 2πrad for the detection of 4.5 fM blaCTX-M-15 gene, and negative control samples did not give a significant signal, confirming that the response contribution comes exclusively from the blaCTX-M-15 target. In addition, the biosensor could detect in real time the blaCTX-M-15 gene interactions at a concentration as low as 0.45 fM, directly and without pre-or post-amplification steps (see Figure 4C). From the signals obtained for each concentration, a calibration curve was generated. The limit of detection, calculated from the linear fitting of the representation of the phase variation vs. DNA concentrations, and considering the minimum measurable signal set as three times the standard deviation of the baseline (8.5 × 10 −5 × 2πrad), yielded a value of only 30 aM (~10 5 CFU mL −1 ), and the limit of the quantification (LOQ) was 250 aM. The data showed a good coefficient of determination (R 2 = 0.994), which is considered as a very encouraging result taking into account the high complexity of the sample tested (see Figure 4D). These results confirmed that the methodology was highly sensitive and specific for detecting fragmented DNA targets.  After the preparation steps, the samples of the blaCTX-M-15 gene, with concentrations ranging from 0.45 to 4.5 fM, were sequentially flowed over the biosensor surface. Figure 4B shows the evaluation of the specificity of the blaCTX-M-15 probes in the BiMW biosensor by monitoring the sensor signal after injection of different negative controls. We tested non-specific DNA (< 1000 bp) from P. aeruginosa at a concentration of 3.4 fM and from E. coli lacking blaCTX-M-15 gene at a concentration of 2.9 fM. These concentrations of the negative controls are similar to the highest concentration of the blaCTX-M-15 target used. These signals show a phase variation of 0.25 × 2πrad for the detection of 4.5 fM blaCTX-M-15 gene, and negative control samples did not give a significant signal, confirming that the response contribution comes exclusively from the blaCTX-M-15 target. In addition, the biosensor could detect in real time the blaCTX-M-15 gene interactions at a concentration as low as 0.45 fM, directly and without pre-or post-amplification steps (see Figure 4C). From the signals obtained for each concentration, a calibration curve was generated. The limit of detection, calculated from the linear fitting of the representation of the phase variation vs. DNA concentrations, and considering the minimum measurable signal set as three times the standard deviation of the baseline (8.5 × 10 −5 × 2πrad), yielded a value of only 30 aM (~10 5 CFU mL −1 ), and the limit of the quantification (LOQ) was 250 aM. The data showed a good coefficient of determination (R 2 = 0.994), which is considered as a very encouraging result taking into account the high complexity of the sample tested (see Figure 4D). These results confirmed that the methodology was highly sensitive and specific for detecting fragmented DNA targets.

Carbapenemase NDM-5
In order to detect the presence of the blaNDM-5 gene in E. coli samples and compare it with the blaCTX-M-15 gene, we employed a forward probe with a short nucleotide spacer. Moreover, to assess efficiency and reproducibility among BiMW sensor devices, the detection of the blaNDM-5 gene was done using two different sensors (BiMW1 and BiMW2). The biosensor BiMW 1 was calibrated with genomic samples prepared following the same pre-treatment, and after thermal denaturation, genomic DNA was tested in triplicates at different concentrations (see Figure 5A). The lowest concentration employed (0.6 fM) produced a phase variation of 0.035 × 2πrad, while the highest produced a phase variation of 0.20 × 2πrad. These results demonstrate the capability of the BiMW biosensor to detect the blaNDM-5 gene at very low concentration without the need for any signal amplification or previous PCR amplification.
Once we demonstrated the recognition of the blaNDM-5 gene, we confirmed the specificity of the assay by evaluating a P. aeruginosa DNA as negative control (3.4 fM). As shown in Figure 5B, the designed probe showed a high specificity for the sample with the blaNDM-5 gene from E. coli, and a negligible signal for the negative control. As previously observed with the blaCTX-M-15 gene, the response compared with the negative control was significantly higher (0.21 × 2πrad for blaNDM-5 gene and 0.004 × 2πrad for the negative control), which confirms the specificity of the probes. The resulting calibration curve is shown in Figure 5C, from which a limit of detection of 28 aM (~10 5 CFU mL −1 ) and LOQ of 234 aM were calculated. The data showed a good coefficient of determination (R 2 = 0.996).

Conclusions
We have demonstrated the efficiency and ultra-sensitivity of a BiMW nanophotonic biosensor for the detection of multidrug resistant genes directly from bacterial cultures of Escherichia coli. Specifically, we implemented the biosensor for the detection of blaCTX-M-15 and blaNDM-5 antimicrobial-resistant gene sequences. This BiMW biosensor methodology is fast, ultra-sensitive As Figure 5 shows, we also evaluated the reproducibility of the results of the evaluation of the blaNDM-5 gene in another BiMW sensor device (BiMW2), obtaining results similar to those obtained with BiMW1 ( Figure 5D) for the same evaluated concentrations. The specificity of the BiMW2 biosensor was also evaluated with a Pseudomonas DNA negative control (3.4 fM) ( Figure 5E). The calibration curve showed a good linear fitting (R 2 = 0.998), obtaining a limit of the detection of 24 aM and LOQ of 205 aM. As Figure 5F shows, these results and the real-time signals are comparable with the BiMW1 results (LOD: 28 aM).
The BiMW biosensor for the blaNDM-5 gene detection demonstrated high sensitivity, real-time monitoring, and reproducibility in two BiMW sensor devices employed for a range of very low concentrations, without the need for any amplification or labeling steps. The designed strategy can be further expanded to evaluate any other relevant resistance sequence genes present in other pathogens.

Conclusions
We have demonstrated the efficiency and ultra-sensitivity of a BiMW nanophotonic biosensor for the detection of multidrug resistant genes directly from bacterial cultures of Escherichia coli. Specifically, we implemented the biosensor for the detection of blaCTX-M-15 and blaNDM-5 antimicrobial-resistant gene sequences. This BiMW biosensor methodology is fast, ultra-sensitive (attomolar levels), reproducible, and has the potentially to be extended to a wide variety of genes from other pathogens.
Direct bacterial genomic DNA detection is an attractive goal for diagnostics, allowing faster analytical responses and cost reduction of the assays, compared with current available methods such as PCR. Our strategy involved the immobilization of two probes (forward and reverse) to capture both DNA strands, as done in PCR. This methodology provides an extraordinary opportunity for molecular microbiology diagnosis, as this biosensor provides considerable advantages over PCR, particularly in terms of assay time and cost, and could be easily adapted for point-of-care applications. This BiMW biosensor, with average LOD in the range of 25 aM and LOQ of 205 aM, represents one of the most sensitive approaches for whole genomic DNA detection without amplification or labeling steps. In addition, the reproducibility was confirmed with two BiMW sensor devices, and discrimination between different genomic DNA from several bacteria was achieved.
In view of the high sensitivity exhibited by the BiMW biosensor, it could potentially be used for the early detection of infections caused by multidrug resistant bacteria, contributing to the early implementation of accurate therapeutic interventions. Funding: This research was supported by CERCA program/Generalitat de Catalunya. ICN2 is supported by the Severo Ochoa Program from Spanish MINECO (Grant No. SEV-2017-0706). The VHIR-HUVH is supported by Plan Nacional de I+D+i 2013-2016 and Instituto de Salud Carlos III, Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad, Spanish Network for Research in Infectious Diseases (REIPI RD16/0016/0003), co-financed by the European Development Regional Fund "A way to achieve Europe".