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Article

The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Treated with Antiglaucoma Eye Medications

1
Department of Ophthalmology, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, Japan
2
Department of Ophthalmology, Keio University School of Medicine, Shinjuku-ku, Tokyo 160-8582, Japan
3
Department of Advanced Aging Medicine, Chiba University Graduate School of Medicine, Chiba 260-8670, Japan
4
Cornea Center Eye Bank, Tokyo Dental College, Ichikawa general hospital, Ichikawa, Chiba 272-8513, Japan
*
Author to whom correspondence should be addressed.
Diagnostics 2020, 10(1), 20; https://doi.org/10.3390/diagnostics10010020
Received: 6 September 2019 / Revised: 18 December 2019 / Accepted: 30 December 2019 / Published: 1 January 2020
(This article belongs to the Special Issue Dry Eye and Ocular Surface Disorders)
Anti-glaucoma eye drop treatment often induces dry eyes and can lead to poor medication adherence. This study aimed to investigate the effects of 3% diquafosol sodium eye drops on tear function and the ocular surface epithelium in Sod1−/− mice after treatment with anti-glaucoma eye drops. The mice were divided into four groups: group 1, control group; group 2, anti-glaucoma eye drop; group 3, anti-glaucoma eye drops followed by a secretagogue eye drop (3% diquafosol); and group 4, simultaneous anti-glaucoma and secretagogue eye drop. Mice underwent assessments of tear quantity, tear film breakup time, and vital staining score. Mice in groups 3 and 4 showed significantly better tear stability and lower corneal staining scores than mice in group 2 after eye drop instillations (p < 0.05). Mice in group 4 showed significantly better tear stability, lower corneal staining scores, and higher goblet cell densities than those in group 1 after eye drop instillations (p < 0.05). The conjunctival epithelium showed stratification and abundance of Muc5AC-positive goblet cells in group 4, whereas thinning with desquamation was observed with a few goblet cells in group 2. Thus, simultaneous administration of 3% diquafosol sodium eye drops with topical anti-glaucoma drops showed favorable effects on tear stability and the corneal epithelium against the ocular surface toxicity inflicted by the anti-glaucoma eye drops. View Full-Text
Keywords: dry eye; anti-glaucoma eye drops; 3% diquafosol sodium eye drops; Sod1−/− mice dry eye; anti-glaucoma eye drops; 3% diquafosol sodium eye drops; Sod1−/− mice
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MDPI and ACS Style

Yagi-Yaguchi, Y.; Kojima, T.; Higa, K.; Dogru, M.; Ibrahim, O.M.; Shimizu, T.; Tsubota, K.; Shimazaki, J. The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Treated with Antiglaucoma Eye Medications. Diagnostics 2020, 10, 20. https://doi.org/10.3390/diagnostics10010020

AMA Style

Yagi-Yaguchi Y, Kojima T, Higa K, Dogru M, Ibrahim OM, Shimizu T, Tsubota K, Shimazaki J. The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Treated with Antiglaucoma Eye Medications. Diagnostics. 2020; 10(1):20. https://doi.org/10.3390/diagnostics10010020

Chicago/Turabian Style

Yagi-Yaguchi, Yukari, Takashi Kojima, Kazunari Higa, Murat Dogru, Osama M. Ibrahim, Takahiko Shimizu, Kazuo Tsubota, and Jun Shimazaki. 2020. "The Effects of 3% Diquafosol Sodium Eye Drops on Tear Function and the Ocular Surface of Cu, Zn-Superoxide Dismutase-1 (Sod1) Knockout Mice Treated with Antiglaucoma Eye Medications" Diagnostics 10, no. 1: 20. https://doi.org/10.3390/diagnostics10010020

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