Successful Production of Offspring Derived from Phospholipase C Zeta-Deficient Sperm by Additional Artificial Activation

During mammalian fertilization, repetitive rises of intracellular calcium called calcium oscillations are required for full activation of oocytes. Therefore, oocytes such as round spermatid injected or somatic cell nuclear transferred require additional artificial activation which mimics the calcium oscillations. It is well recognized that sperm specific phospholipase C (PLCζ) is a strong candidate as the sperm factor which can induce calcium oscillations and, at least in mammals, the genetic mutation of PLCζ in human causes male infertility due to the lack of calcium oscillations in the oocytes. Recent studies showed that the sperm lacking PLCζ (Plcz1−/−) still could induce rise(s) of intracellular calcium in the oocytes after IVF but not intracytoplasmic sperm injection (ICSI). In the ICSI oocytes, no pronuclear formation or development to the two-cell stage was observed. However, it is still unclear whether additional activation treatment can rescue the low developmental ability of Plcz1−/−-sperm-derived oocytes after ICSI. In this study, we examined whether oocytes injected with a Plcz1−/− sperm can develop to term by additional artificial activation. In oocytes injected a Plcz1−/− sperm and Plcz1−/− and eCS (another candidate of the sperm factor) double knockout sperm (Plcz1−/−eCS−/−), the rates of pronuclear formation were very low (2.0 ± 2.3% and 6.1 ± 3.7%, respectively) compared to control (92.1 ± 2.6%). However, these rates were dramatically improved by additional procedures of PLCζ-mRNA injection or SrCl2 treatment (Plcz1−/− sperm + PLCζ mRNA, Plcz1−/− sperm + SrCl2 and Plcz1−/−eCS−/− sperm + PLCζ mRNA; 64.2 ± 10.8%, 89.2 ± 2.4% and 72.6 ± 5.4%, respectively). Most of the oocytes were developed to the two-cell stage. After embryo transfer, healthy pups were obtained in all these groups (Plcz1−/− sperm + PLCζ mRNA:10.0 ± 2.8%, Plcz1−/− sperm + SrCl2:4.0 ± 4.3% and Plcz1−/−eCS−/− sperm + PLCζ mRNA: 10.0 ± 5.7%). The rate in Plcz1−/− sperm + SrCl2 group was significantly lower than that in control (26.0 ± 2.4%). Taken together, our present results show that additional activation treatment such as SrCl2 and PLCζ mRNA can fully support to develop to term even in oocyte injected Plcz1−/− sperm. In addition, PLCζ-induced oocyte activation is more suitable for successful development to term compared to that such as phenomenon induced by SrCl2. These findings will contribute to improvement for male-dependent human infertility and reproductive technologies in other mammalian species.


Introduction
During mammalian fertilization, the penetration of sperm into the ooplasm triggers repetitive increases in the intracellular calcium concentration, i.e., 'calcium oscillations.' These oscillations induce events such as the resumption of meiosis at the metaphase-II (MII) stage, the exocytosis of cortical granules, and the formation of the pronucleus (PN), Life 2023, 13, 980 2 of 13 which together are called 'oocyte activation' [1][2][3]. It has thus been expected that calcium oscillation-dependent oocyte activation is indispensable for development from embryo to term.
Saunders and his colleagues [4] proposed that phospholipase Cζ (PLCζ) might be the sperm-derived oocyte-activating factor that can induce calcium oscillations during fertilization in the mouse. A microinjection of RNA encoding PLCζ [4] or recombinant PLCζ protein [5] induced calcium oscillations. In addition, the calcium oscillation-inducing activity of sperm extracts was lost when the sperm extracts were pretreated with an antibody against PLCζ [4]. The level of PLCζ that is able to produce fertilization-like calcium oscillations in a single oocyte is in the same range as the single-sperm content of PLCζ [4]. To date, PLCζ has been detected in many mammalian and some non-mammalian species, including mouse [4,6], rat [5], human [5,7], cynomolgus monkey [7], cow [8,9], pig [10,11], horse [12] and medaka fish [5]. Taken together, at least in mammals, it has been believed that PLCζ is a strong candidate as the sperm factor which conserved in many mammalian species. The sperm-induced calcium oscillations are generated by a release of calcium from 1,4,5-inositol trisphosphate (IP 3 )-sensitive intracellular calcium stores [13,14]. Since IP 3 is generated by the PLCζ-mediated hydrolysis of phosphatidyl inositol 4,5-bisphosphate (PI(4,5)P 2 ), the activation of PLCζ and the production of IP 3 are required for the induction of calcium oscillations. Genetic mutations of PLCζ cause male infertility in humans [15][16][17][18]. These findings strongly suggest that PLCζ functions as a sperm factor that induces calcium oscillations, and that PLCζ is thus indispensable for oocytes' activation and further development, at least in mammals.
Two research groups have reported their production of a Plcz1 (the gene name of PLCζ)-deficient (Plcz1 −/− ) mouse [19,20]. Surprisingly, both groups reported that the Plcz1 −/− male mice were not infertile and that their sperm can fertilize oocytes even with a lack of calcium oscillations. These results led us to hypothesize that PLCζ is dispensable for oocytes' activation and further embryonic development. It was also reported that oocytes were activated when Plcz1 −/− sperm were used for in vitro fertilization, but not when intracytoplasmic sperm injection (ICSI) was applied [19,20], suggesting that oocyte activation via the fusion of the oocyte membrane is possible even in Plcz1 −/− sperm.
Many reproductive technologies such as ICSI and round spermatid injection (ROSI) have been developed and used in the study of human reproduction. The successful production of cloned animals derived from somatic cell nuclear transfer (SCNT) was reported in several mammalian species [21][22][23][24]. Since it is known that the injection of a round spermatid cannot induce the activation of oocytes and since sperm are not used for SCNT, it is necessary to devise artificial activation procedures that mimic the rise(s) of intracellular calcium at fertilization in order to achieve oocyte activation. In studies seeking to accomplish the artificial activation of oocytes in mice, strontium chloride (SrCl 2 ) is used for technologies such as ROSI [25] and SCNT [26] because it is believed that SrCl 2 treatment can produce fertilization events such as calcium oscillations in oocytes [27]. However, it has not been known whether SrCl 2 treatment can induce oocyte activation and subsequent embryonic development in addition to calcium oscillations at fertilization, or whether oocytes injected with Plcz1 −/− sperm can develop to term when subjected to artificial activation.
In addition to PLCζ, it is thought that extra-mitochondrial citrate synthase (eCS) is another known candidate as the sperm factor inducing oocyte activation [28]. CS is a core enzyme of the mitochondrial tricarboxylic acid cycle, which directly controls cellular function [28]. Previous reports showed that eCS is produced and abundant in the sperm head and the injection of eCS mRNA induced calcium oscillations in mouse oocytes [28]. eCS deficient (eCS −/− ) male mice were initially fertile but calcium oscillations were delayed in oocytes fused with eCS deficient (eCS −/− ) sperm, despite normal expression of PLCζ [28]. In addition, eCS −/− males were fertile, but their litter size was remarkably reduced in 6month-old males [28]. These results suggest eCS also may have a role in calcium oscillations, Life 2023, 13, 980 3 of 13 oocyte activation and male fertility independent of PLCζ. However, the effect of deletion of both PLCz1 and eCS on oocyte activation still remains unclear.
We conducted the present study to determine whether SrCl 2 treatment or PLCζ mRNA injection after ICSI using Plcz1 −/− sperm can restore oocyte activation and the subsequent embryonic development. We also examined whether Plcz1 −/− eCS −/− sperm can induce oocyte activation or not when the sperm was used for ICSI.

Materials
All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise stated.

Animals
The mice used in this study were C57BL/6 female mice (8-10 weeks of age) for the collection of metaphase II (MII) oocytes, and C57BL/6 males (12-24 weeks of age) were used for the sperm collection. The female mice were purchased from SLC Inc. (Hamamatsu, Japan). Mature female ICR mice (12-14 weeks of age) were used as recipients of the embryo transfer. Vasectomized male ICR mice (20-30 weeks of age) were used to induce pseudopregnancies. The surrogate pseudopregnant ICR females that were used as recipients of the embryos were mated with vasectomized ICR males whose sterility had been confirmed. Plcz1 +/− [20] and eCS +/− mice [28] were transferred from RIKEN BioResource Research Center (Ibaraki, Japan) and National Center for Child Health and Development (Tokyo, Japan), respectively. Plcz1 −/− eCS −/− double knock out mice were produced by crossing with Plcz1 +/− and eCS +/− mice.
On the day of the experiments or after all experiments were completed, the mice were sacrificed by CO 2 inhalation or cervical dislocation and used for further experiments. The mice were housed in an environmentally controlled room with a 12-h dark/12-h light cycle at a temperature of 23 ± 2 • C and humidity of 55 ± 5% with free access to a laboratory diet and filtered water.

Sperm Cryopreservation and Sperm Motility Analysis
Spermatozoa obtained from the cauda epididymis were counted and then cryopreserved. To thaw sperm, a straw was warmed for 1 min in water bath (37 • C) after keeping for 10 sec in air. After thawing, spermatozoa were incubated in a drop of Human Tubal Fluid (HTF) medium at 37 • C under 5% CO 2 . Forty-five minutes after incubation, spermatozoa were collected from the top of the drop. Sperm motility was analyzed using the SMAS sperm motility analysis system (DITECT Corporation, Tokyo, Japan).

Oocyte Preparation
Superovulation was conducted as previously described [29]. Cumulus oocyte complexes (COCs) at the metaphase-II stage were collected from the oviducts of the mice Life 2023, 13, 980 4 of 13 that were superovulated by an i.p. injection of 5 IU equine chorionic gonadotropin (eCG; Nippon Zenyaku Kogyo, Tokyo, Japan) followed by 5 IU human chorionic gonadotropin (hCG; Asuka Pharmaceutical Co., Tokyo, Japan) 48 h later. Twelve to fourteen hours after hCG injection, the females were sacrificed and their oviductal ampullae were removed. The oviductal ampullae were placed in oil, and COCs were collected from the oviductal ampullae and then transferred to HEPES-buffered CZB (HEPES-CZB) medium [30]. To disperse the cumulus, the COCs were transferred into a 100-µL droplet of HEPES-CZB medium containing 0.1% hyaluronidase (Sigma, St. Louis, MO, USA) for 3 min. The cumulus-free oocytes were washed twice and then moved to a 20-µL droplet of CZB medium [31] for further experiments.

Preparation of Mouse PLCζ mRNA and Its Microinjection
We used pCS2 + -mousePlcz1 [10] as the template for mRNA synthesis. mRNA was synthesized as described. The in vitro transcription was carried out using an mMESSAGE MACHINE sp6 kit (Life Technologies, Carlsbad, CA, USA) according to the manufacturer's instructions. The synthesized RNA was polyadenylated with a poly(A) tailing kit (AM1350, Life Technologies). The RNA with poly(A) tail was precipitated using lithium chloride and dissolved in nuclease-free water. After the concentration was measured with a spectrophotometer (NanoDrop ND-1000, NanoDrop Technologies, Wilmington, DE, USA), 500 ng/µL aliquots were stored at −80 • C until further use.
Before microinjection, PLCζ mRNA was diluted with nuclease-free water to 1 ng/µL. The microinjection (~10 pL/oocyte) of mRNA into oocytes was executed using a piezodriven micropipette (Prime Tech, Ibaraki, Japan) as previously reported [29]. Briefly, each microinjection was performed in HEPES-CZB medium on an inverted microscope (Olympus, Tokyo, Japan) with a micromanipulator (Narishige, Tokyo, Japan). The zona pellucida and cytosolic membrane were penetrated with a piezo drive. After the mRNA injection, the oocytes were kept in HEPES-CZB medium at room temperature for 10 min before culturing in CZB medium.

Production of Fertilized Embryos by Intracytoplasmic Sperm Injection
ICSI was performed as described [30,32]. The frozen/thawed sperm were retrieved into HEPES-CZB medium and were washed three times with the same medium to remove cryoprotectant. The frozen sperm head derived from B6 (WT), Plcz1 −/− , and Plcz1 −/− eCS −/− male mice was separated from the tail by the application of several piezo pulses to the head-midpiece junction (the neck) in 10% Polyvinylpyrrolidone Solution with HAS (FUJIFILM Wako Chemicals, Tokyo, Japan). The separated head was then injected into the oocyte collected from a WT female mouse in HEPES-CZB medium. After 10 min of recovery at room temperature, the oocyte was cultured in CZB medium for preimplantation development. For SrCl 2 treatment, some of the sperm-injected oocytes were activated using 5 mM SrCl 2 in Ca 2+ -free CZB medium for 30-60 min.

In Vitro Culture and Embryo Transfer
After the ICSI, mRNA injection, and SrCl 2 treatment, the morphologically-survived oocytes were incubated in CZB medium at 37 • C in 5% CO 2 . Pronuclear formation was checked 6 h after activation/injection. The embryos were washed three times in KSOM-AA and then transferred into 100 µL of KSOM-AA and were cultured up to blastocysts at 37.5 • C under 5% CO 2 in air. Cleavage and blastocyst formation of the oocytes were evaluated at 18 h and 114 h post-ICSI, respectively. For the experiments of embryo transfer, embryos at the two-cell stage were transferred to a day-0.5 pseudopregnant mouse that had been mated with a vasectomized male the night before transfer. After, approximately 10-15 embryos were transferred into each oviduct. At day 18.5 of gestation, the offspring were delivered by caesarean section and allowed to mature. Offspring rate, offspring weight and placenta weight were examined.

Immunostaining
Blastocysts were used for the immunofluorescence staining applied to evaluate the quality of the embryos [32]. Following three washes in PBS containing 0.1% polyvinyl alcohol (PBS-PVA), blastocysts were fixed for 60 min in PBS-PVA containing freshly prepared 2% (w/v) paraformaldehyde and 0.2% (v/v) Triton X at 24 • C. After fixation, oocytes were washed with PBS-PVA (three times for 15 min each) and oocytes were immersed in PBS-PVA containing 2.5% (v/v) Tween 20 (GE Healthcare Co., Chicago, IL, USA) for 2 min at 24 • C. Blastocysts were washed with PBS-PVA (three times for 15 min each) again, incubated for 2 h in PBS-PVA containing 1% (w/v) BSA (PBS-BSA) at 4 • C and thereafter in blocking buffer (PBS-BSA containing 10% (v/v) goat serum) for 40 min at 24 • C. The blastocysts were incubated in primary antibody overnight at 4 • C. The primary antibodies used were an anti-CDX2 rabbit monoclonal antibody (1:500; BioGenex, Fremont, CA, USA) to detect the TE cells and an anti-NANOG mouse polyclonal antibody (1:500; Abcam, Cambridge, UK) to detect the ICM cells. Alexa Fluor 568-labeled goat anti-rabbit IgG and Alexa Fluor 488-labeled goat anti-mouse IgG (both 1:500; Thermo Fisher Scientific, Waltham, MA, USA) were used as secondary antibodies to detect the ICM and TE cells, respectively. DNA was stained with DAPI (2 µg/mL; Molecular Probes, Eugene, OR, USA).

Statistical Analysis
Statistical analysis was performed using the Statcel 3 software (OMS Ltd., Saitama, Japan). All experiments were analyzed using one-way analysis of variance (ANOVA). The Tukey-Kramer procedure was for multiple comparisons. The parameters calculated as percentages were subjected to arcsine transformation before performing ANOVA, and a p value of <0.05 was considered statistically significant.

The Phenotype of Testes and Sperm Derived from Plcz1
At the beginning of the present study, we have checked the fertility of eCS −/− male mice because a previous study reported that eCS −/− males were fertile, but their litter size was remarkably reduced in 6-month-old males [28]. However, under our experimental condition, we did not confirm a decrease in fertility in eCS −/− male mice even older than 30 weeks (7 months) of age as described above. Our results showed that the averages of pups were 7.9 (n = 21, 12-24 weeks from WT male), 7.2 (n = 20, 12-24 weeks from eCS −/− male) and 7.4 (n = 26, 32-50 weeks from eCS −/− male) (Supplementary Figure S1). Therefore, we used 12-24-week-old Plcz1 −/− eCS −/− mice for the present study. The testes from Plcz1 −/− or Plcz1 −/− eCS −/− male mice were sectioned and stained with hematoxylin and eosin (H&E). Typical results are presented as Figure 1. In both the Plcz1 −/− and Plcz1 −/− eCS −/− testes, male germ cells and spermatozoa were confirmed (Figure 1b,c as they were in the wildtype [WT] testes (Figure 1a)). Indeed, the Plcz1 −/− and Plcz1 −/− eCS −/− male mice were not infertile. These results suggest that the genetic deletion of Plcz1 and that of both Plcz1 and eCS did not affect the production of sperm.

Discussion
PLCζ has been suspected to be the sperm factor that induces oocyte activation [13,33], at least in mammals. Nozawa et al. [20] demonstrated that Plcz1 −/− mouse spermatozoa failed to induce Ca 2+ changes in ICSI; however, healthy pups were obtained after transplantation of the Plcz1 −/− sperm-derived embryos when PLCζ mRNA was injected into the embryos. Our present results also demonstrated the successful production of embryos derived from Plcz1 −/− spermatozoa with the injection of PLCζ mRNA, similar to the findings described by Nozawa et al. These results strongly suggest that (i) PLCζ works per se as a sperm factor, and (ii) its function fully supports the ability to develop to term.
Extra-mitochondrial citrate synthase (eCS), which has been described as another sperm factor [28], was first reported as a sperm factor in newts; the microinjection of eCS into unfertilized newt eggs induced a transient increase in intracellular calcium [34]. A later study using mice showed that (i) eCS was expressed in the sperm head even after the acrosome reaction, and (ii) the injection of eCS mRNA into MII oocytes induced calcium oscillations [28]. These results suggested that eCS is suitable as the sperm factor (as is PLCζ). Our present results demonstrated that an injection of PLCζ mRNA also rescued the developmental ability of the embryos derived from Plcz1 −/− eCS −/− sperm in addition to Plcz1 −/− sperm. In an earlier investigation, the injection of eCS −/− spermatozoa delayed the calcium oscillations, but eCS −/− male mice were fertile [28]. Although only old eCS −/− male mice (more than 24 weeks) showed the decrease in fertility [28], the results from our study demonstrated that the decreased fertility of eCS −/− male mice (even 32-50 weeks) was not observed (Supplementary Figure S1). We do not have any explanation about the difference, but in this study, we used 12-24-week-old male mice as the Plcz1 −/− eCS −/− group. In addition, our preliminary study showed that even Plcz1 −/− eCS −/− male mice were also not infertile and spermatozoa were confirmed in the testes from Plcz1 −/− eCS −/− male mice. Sperm motility and concentration in Plcz1 −/− and Plcz1 −/− eCS −/− groups were also similar to the WT group. In this study, we have not checked the fertility of embryos derived from Plcz1 −/− eCS −/− sperm with old age and cannot rule out the possibility that sperm derived from Plcz1 −/− eCS −/− -aged males can have altered fertilization ability in comparison to the young ones. Further experiments will be conducted to clarify the possibility. These results indicate that eCS is not essential for development to term, at least in the young mouse.
On the other hand, oocytes injected with Plcz1 −/− or Plcz1 −/− eCS −/− sperm did not form pronuclei if they were not treated with additional treatment. Although the intracellular calcium concentration was not measured in this experiment, previous studies already reported that when sperm derived from Plcz1 −/− male mice were used for ICSI, there was no increase in intracellular calcium in the oocytes [19,20]. Based on these facts, it is considered in the present study that the rises of intracellular calcium (including calcium oscillations) was completely suppressed in the oocytes injected with Plcz1 −/− or Plcz1 −/− eCS −/− sperm, resulting in the failure of pronuclear formation. As shown in the previous studies and our unpublished observation, since PLCζ-independent and membrane-fusion-dependent raises of intracellular calcium mechanisms exist, Plcz1 −/− or Plcz1 −/− eCS −/− can be fertilized and induce pronuclear formation in IVF.
The treatment with SrCl 2 in the present study was also demonstrated to rescue the developmental ability of Plcz1 −/− -spermatozoa-derived embryos. Although the rescue experiment using SrCl 2 was not performed for Plcz1 −/− eCS −/− -spermatozoa-derived embryos, the results would be similar to that from Plcz1 −/− -spermatozoa-derived embryos. Treatment with SrCl 2 is widely used to induce oocyte activation for SCNT and ROSI in several mammalian species [35][36][37][38]. Although we observed that the developmental ability of the Plcz1 −/− -sperm-derived embryos treated with SrCl 2 was greater than that of the embryos injected with PLCζ mRNA, the cell numbers of the blastocyst were significantly decreased in the Plcz1 −/− + SrCl 2 group compared to that in the Plcz1 −/− + PLCζ mRNA group. In addition, the offspring rates when PLCζ mRNA was used were superior to those achieved with SrCl 2 for the activation of Plcz1 −/− sperm-derived oocytes. In the previous study [39], using fertilized oocytes that have undergone the first few calcium oscillations, they developed procedures that result either in inhibiting or stimulating the natural pattern of calcium signaling of inseminated oocytes. Although the incidence of development to the blastocyst stage is unaltered by these procedures, fewer offspring are born following embryo transfer, indicating that developmental competence of the blastocysts is reduced [39]. It has been shown that treatment with SrCl 2 can induce the calciumoscillations-like phenomenon that generally occurs during fertilization in mammals [27,40]. It is still unclear whether there is a difference between PLCζ-induced oscillations and SrCl 2 -induced oscillations-like phenomenon. At least, PLC is a hydrolytic enzyme that produces IP 3 and DG but not SrCl 2 . Although further experiments will be required, it seems to be a possibility that the calcium oscillation-like phenomenon caused by SrCl 2 does not fully reproduce PLCζ-derived calcium oscillations. This difference may result in the low rate of the pups derived from Plcz1 −/− sperm-injected + SrCl 2 -treated group. In light of these results, we speculate that PLCζ has essential roles in not only oocyte activation but also embryonic development to term. Further investigations will clarify the detailed functions of PLCζ during fertilization in mammals.

Conclusions
Our present findings demonstrate that additional treatment (i.e., an injection of PLCζ mRNA or SrCl 2 treatment) inducing a calcium-oscillation-like phenomenon can rescue the developmental ability of Plcz1 −/− -spermatozoa-derived embryos. Many reproductive technologies such as ICSI and round spermatid injection (ROSI) have been developed and used in various research and clinical areas to date. The successful production of cloned animals derived from somatic cell nuclear transfer (SCNT) was also reported in several mammalian species [21][22][23][24].
At least in mice, treatment with strontium chloride (SrCl 2 ) is applied for inducing oocyte activation in ROSI [25] and SCNT [26] because it is believed that SrCl 2 treatment can produce fertilization events such as calcium oscillations in oocytes [27]. Our present results suggest injection of PLCζ mRNA seems to be more suitable for oocyte activation compared with SrCl 2 . However, it is required that the subsequent normality of the offspring derived from additional activation treatments will be evaluated in further investigations.
In addition, it has been indicated that male infertility in humans depends on the genetic mutation of Plcz1 −/− , which causes a loss of calcium oscillations, resulting in failed pregnancy after ICSI [41][42][43]. Since injecting PLCζ mRNA will raise ethical issues in a human clinical field, it will be necessary to consider the development of artificial activation methods that more closely resemble normal fertilization in the future. Our current observations will contribute to the improvement of PLCζ-dependent male infertility.
Supplementary Materials: The following supporting information can be downloaded at: https:// www.mdpi.com/article/10.3390/life13040980/s1. Figure S1. Fertility of young and aged eCS −/− mice.  All methods involving animals were performed in accordance with the ARRIVE guidelines.

Informed Consent Statement: Not applicable.
Data Availability Statement: The datasets analyzed during the current study are available from the corresponding author upon reasonable request.