Essential Oil Composition Analysis, Antimicrobial Activities, and Biosystematic Studies on Six Species of Salvia

The essential oil constituents, antimicrobial properties, and biosystematic characteristics (morphological, palynological, and anatomical features) of six Salvia species from different regions of Turkey were investigated qualitatively and quantitatively in this study. The chemical composition of the essential oils of dried aerial parts of Salvia species, i.e., S. absconditiflora, S. ceratophylla, S. multicaulis, S. verbenaca, S. viridis, and S. syriaca were analyzed by GC-MS. The main constituents of the six Salvia species studied were 1,8-cineol, caryophyllene oxide, spathulenol, and borneol in different ratios. The antimicrobial activity of the essential oil extracted from the aerial parts of species of the genus Salvia was tested by the disc diffusion method. The essential oils of Salvia species showed different antimicrobial activity against the studied microorganisms. The highest antimicrobial activity against E. coli was observed in S. multicaulis and the highest antimicrobial activity against K. pneumoniae was observed in S. verbenaca. The morphology of the stem, leaf, bract, and flower structures of the Salvia species were analyzed in this study. Anatomical investigations focused on the root, petiole, and stem in more detail. Our research has broadened the criteria of anatomical characters unique to the Salvia species. Under light microscopy, the pollen grains of the six species belonging to Salvia were isopolar and radially symmetrical. The properties of the essential oil constituents, antimicrobial properties, and biosystematic data obtained in this study contribute to the bioactive and biosystematic studies of Salvia species used for food, pharmaceutical, and cosmetic purposes.


Introduction
Salvia L. gets its name from the Latin word "Salvare", which means "to heal or treat" [1]. Salvia belongs to the Salvinae subtribe, Lamiaceae family, Lamiales order of dicotyledonous. Salvia species have been identified in the group of commercial, medicinal, and aromatic herbs and are one of the various centers in Turkey, especially the Anatolia region [2]. Therefore, Turkey exhibits the greatest expansion of sage and is also one of the countries that widely consume it for commercial purposes [3].
Salvia is an important genus in the Lamiaceae family with approximately 1000 species worldwide, which contains many species producing secondary metabolites [4]. According to the most recent Flora of Turkey, there are more than 86 Salvia taxa in Turkey, while other studies indicate that it has increased to about 100 species [5]. Turkish Salvia species are discussed in seven sections, including Aethiopis, Drymosphace, Hymenosphace, Horminum, Hemisphace, Plethiosphace, and Salvia. In the current study, the divisions of the sections are as follows: S. absconditiflora (Montbret & Aucher ex Benth.) (syn. Salvia cryptantha Montbret & Aucher ex Benth), S. multicaulis Vahl. in the Hymenosphace sectionss, S. syriaca L., S. verbenaca L., and S. ceratophylla L. in the Aethiopis section, and S. viridis L. in the Horminum section [2,[6][7][8].
Molecular characterization studies carried out in recent years give important ideas about the systematics of species [9][10][11][12][13]. Genetic characterization studies among Salvia species have also revealed a large genetic variation among species. Similarly, flow cytometry and genome size determination studies have shown that genetic diversity is very rich among samples collected from different locations of the same species [4].
For thousands of years, the Salvia genus has been used in traditional medicine and is utilized in a broad variety of commercial and pharmaceutical goods, particularly in the production of essential or volatile oils and flavoring compounds, as well as in the food and cosmetics sectors. Essential oil components of Salvia species have an important place in the medicinal, aromatic plants market. Several studies have been conducted on the essential oil of this genus [14][15][16]. The chemical composition of Salvia L. was investigated by Gas Chromatography-Mass Spectrometry system with major compounds including caryophyllene oxide, spathulenol, α-copaene, germacrene D, and β-pinene [17]. Despite the fact that the majority of Salvia species are employed in traditional herbal medicine, studies have shown that they also exhibit anti-inflammatory, antibacterial, antioxidant, anticancer, and antidiabetic properties [12,14,15,[18][19][20][21]. Additionally, these essential oils have been utilized to treat eczema, psoriasis, and asthma diseases [22]. S. absconditiflora species, represented in group C in the Flora of Turkey, is an endemic species. These endemic species have been researched and consumed as herbal tea in Turkey [23]. S. absconditiflora is thought to have a strong antioxidant effect because of its high phenolic and flavonoid content. Additionally, S. absconditiflora was said to have a strong cytotoxic effect on cancer cells [15]. Furthermore, S. syriaca is utilized in the food industry, the pharmacology and cosmetic industry, and it is also used to treat animals [24]. Moreover, S. ceratophylla is traditionally consumed to cure microbial infections, cancer, and urinary tract problems in Jordan [25,26]. However, some Salvia species have antimicrobial activity when extracted from above-ground soil [27,28].
Some Salvia species were compared with anatomical, morphological, and pollen features [29] and the findings showed that there were similarities and differences among the species. Thus, several of these important features, including the form of the calyx, corolla, and stamen, may be utilized to discriminate across infrageneric categories. Calyx and corolla shape, bract morphology and structure as well as inflorescence type are important diagnostic characteristics of taxonomic value regarding the six studied Salvia species, i.e., S. absconditiflora, S. ceratophylla, S. multicaulis, S. verbenaca, S. viridis, and S. syriaca. The morphology of the stem, leaf, bract, and flower structures of the Salvia species were analyzed in this study. Pollen characteristics of the family Lamiaceae have considerable taxonomic importance and the classification of genera in Labiatae has been revised [30,31], with Salvia placed within the subfamily Nepetoideae because it had hexacolpate pollen [29].
This study aimed to contribute to both the systematics and bioactivity of the genus by investigating the qualitative and quantitative characteristics of some biosystematic features (morphological, palynological, and anatomical features) as well as the essential oil constituents and antimicrobial properties of six Salvia plants collected from different regions of Turkey.

Plant Material
Plant samples were collected from the locations indicated in Table 1. All plants were collected during the flowering period and in the morning hours [32]. For essential oil studies, approximately 200 g of plant samples were dried in a place out of the sun. For anatomical studies, the plants were stored in 70% alcohol in the area where they were collected. For morphological and palynological examinations, 20 plant samples were selected from each taxon; the samples were dried and kept as herbarium specimens at Bingol University.

Isolation of Essential Oils and GC-MS Analysis
The plants used in this research were air-dried. The oil from the plants was extracted using the hydrodistillation technique. Using Clevenger equipment, three hours of hydrodistillation were performed on the 100 g of air-dried aerial plant materials. The organic layer in the gathering vial was transferred into the GC/GC-MS equipment once the distillation process was accomplished.
The GC-MS was used to examine the essential oil. The instrument was an HP 6890 model. The mass range was between 40 and 330 m/z, and the ionization energy was 70 eV. A column HP-5 MS (30 m 0.25 mm i.d., film thickness 0.25 m) capillary column with a column flow rate (transporter gas helium) was implemented. Helium is used as the carrier gas, with a steady column flow rate of 1 mL/min. The settings for the Column Oven Temperature procedure were 40 • C and a hold time of 2 min at a temperature of 3 • C/min. 240 • C was the final degree. The flow rate was set to 1 L, and the split mode was chosen (split ratio 1:10 or 1:100). A 3.5 min buffer hold was applied to hexane samples.
The mass spectrometric settings were full scan mode, 20,000 amu/s scan speed, and 50 spectra per sample frequency. Temperatures at the contact and ion source were 250 • C and 200 • C, etc.
Alkanes were used as standards to compute the retention indices (RI). By comparing the retention times (RT), mass spectra, and RI of the essential oils to those described in the literature (NIST 20 and Wiley Libraries) and MS libraries (Wiley), the chemical components of the essential oils were identified. Traditional library searches just compare spectra rather than taking retention parameters into account. In this study, libraries were searched using a combination of storage indexes, which made compound identification simpler and more accurate. The device's retention index spectrum libraries were also utilized in this study. The same analytical procedure as the identical column provided in the library was applied for better results. Table 2 details the essential oil constituents that have been identified.

PCA (Principal Component Analysis)
Multivariate analysis was performed to determine the structure of variability and to calculate differences between groups. Complete data sets were used for these analyses. To determine the commonalities between the measurement units, the UPGMA (unweighted pair-group average linkage) clustering approach based on Pearson distances was used ( Figure 1). The chemical components of the essential oils of the Salvia species were considered as variables. The chemical components of the different samples were evaluated using cluster analysis (CA) and principal component analysis (PCA). The same weight was given to the non-standardized statistics as previously reported.

Antimicrobial Investigations
The disk diffusion technique was used to test the plant essential oil's antibacterial activity [36]. Yeast strains (Candida albicans and Candida glabrata) were cultured in malt extract broth for 48 h at 25 ± 1 °C, whereas bacteria strains (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus megaterium) were incubated in nutrient broth for 24 h at 35 ± 1 °C. At a rate of 1%, the bacteria and yeast cultures made in broth were added to Mueller Hinton Agar and Plate Count Agar, respectively (10 6 bacteria ml, 10 4 yeast ml, 10 4 fungi mL −1 ). 25 cc of the cultures were added to sterile 9 cm diameter petri plates after being well shaken. The medium's homogeneous dispersion was accomplished. On the solidified agar medium, antimicrobial discs with a 6 mm diameter that were each impregnated with 100 μL of essential oil were lightly positioned. The plates infected with bacteria were incubated at 37 ± 0.1 °C for 24 h and the plates inoculated with yeast were incubated at 25 ± 0.1 °C for 72 h after being kept the petri dishes generated in this manner at 4 °C for 1.5 to 2 h. Different standard discs were used as controls for yeasts (Nystatin 100 mg/disc) and bacteria (Streptomycin sulfate 10 mg/disc). Inhibitory zones were measured in millimeters ( Figure 2).

Antimicrobial Investigations
The disk diffusion technique was used to test the plant essential oil's antibacterial activity [36]. Yeast strains (Candida albicans and Candida glabrata) were cultured in malt extract broth for 48 h at 25 ± 1 • C, whereas bacteria strains (Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus, and Bacillus megaterium) were incubated in nutrient broth for 24 h at 35 ± 1 • C. At a rate of 1%, the bacteria and yeast cultures made in broth were added to Mueller Hinton Agar and Plate Count Agar, respectively (10 6 bacteria mL, 10 4 yeast mL, 10 4 fungi mL −1 ). 25 cc of the cultures were added to sterile 9 cm diameter petri plates after being well shaken. The medium's homogeneous dispersion was accomplished. On the solidified agar medium, antimicrobial discs with a 6 mm diameter that were each impregnated with 100 µL of essential oil were lightly positioned. The plates infected with bacteria were incubated at 37 ± 0.1 • C for 24 h and the plates inoculated with yeast were incubated at 25 ± 0.1 • C for 72 h after being kept the petri dishes generated in this manner at 4 • C for 1.5 to 2 h. Different standard discs were used as controls for yeasts (Nystatin 100 mg/disc) and bacteria (Streptomycin sulfate 10 mg/disc). Inhibitory zones were measured in millimeters ( Figure 2).

Morphological Investigations
The taxonomic characters of the Salvia species were prepared in 20 samples according to Flora of Turkey and some significant articles [2,5,6]. The morphological and morphometrical characters are presented in the result section. Morphological measurements were

Morphological Investigations
The taxonomic characters of the Salvia species were prepared in 20 samples according to Flora of Turkey and some significant articles [2,5,6]. The morphological and morphometrical characters are presented in the result section. Morphological measurements were taken with calipers. All of the morphological measurements were performed using Hierarchical clustering analysis using SPSS software version 21, and the resulting dendrogram was shown.

Anatomical Investigations
Anatomical investigations were conducted using an average of twenty specimens unbroken in 70% alcohol. The cross-sections of stem, root, and petiole organs were cut with a razor, and sections were stained with Alcian blue for cellulose substances and safranine O for polymer substances within the quantitative relation of 3:2. For staining, the sections were placed in the ready dye for five minutes [37,38]. Furthermore, sections were examined and measured using a Euromex CMEX-10 PRO light microscope.

Palynological Investigations
Pollens were generated using the Ertman technique, using samples from the light microscopy [30]. A few changes were made to the acetolysis procedure after then. Plant matter was cursed for one to two minutes using a glass rod and one to two drops of acetic acid in the slide. A needle was used to clear the particles off the slide. A coverslip was placed over the slide after applying a drop of glycerin jelly. Each specimen received 4-5 prepared slides in total. Polar axis (P), equatorial axis (E), colpus length (Clg), colpus width (Clt), exine thickness (Ex), and aperture width (Ap) were measured from at least 20 completely evolved grains according to the pattern beneath a Euromex CMEX-10 PRO light microscope (100×). These measurements are reported in Table 3, and micrographs in Figure 3. The terminology used is mainly from Faegri & Iversen, Ertman, and the study of Kılıç et al. [39][40][41].
For scanning electron microscopy (SEM) Salvia species pollen slides were prepared using the techniques of Majeed et al. [42]. Pollen was acetolyzed before being suspended in 90% ethanol for SEM. They were then placed on metallic stubs that had been gold-palladium coated. Pollen electron micrographs are taken with an SEM (Model JEOL JSM5910). Results from pollen SEM are summarized in Figure 4.    All of the pollen measurements were performed using Hierarchical clustering analysis performed using SPSS software version 21 and the resulting dendrogram was shown in Figures 5 and 6.

Essential Oil Components
Qualitative and quantitative differences were found in the essential oil analysis of the six Salvia species the essential oils of S. absconditiflora, and S. ceratophylla 29, and 28 components were identified representing 100% and 98.62% of the oils, respectively. The aerial part of S. absconditiflora and S. ceratophylla were hydrodistilled, obtaining yields of 0.97% and 0.75% (w/w) of yellowish oils, respectively. The aerial parts of the S. multicaulis and S. verbenaca were hydrodistilled, obtaining yields of 0.97% and 0.95% (w/w) of yellowish oils, respectively. In the essential oils of this species, 27 and 25 components were identified representing 92.60% and 94.26% of the oils, respectively. S. viridis has 30 components (0.78 w/w). The essential oils of S.viridis have 29 components and S. syriaca was identified with 31 components. Additionally, this species was representing 91.54% and 91.02% of the oils, respectively. The

Essential Oil Components
Qualitative and quantitative differences were found in the essential oil analysis of the six Salvia species the essential oils of S. absconditiflora, and S. ceratophylla 29, and 28 components were identified representing 100% and 98.62% of the oils, respectively. The aerial part of S. absconditiflora and S. ceratophylla were hydrodistilled, obtaining yields of 0.97% and 0.75% (w/w) of yellowish oils, respectively. The aerial parts of the S. multicaulis and S. verbenaca were hydrodistilled, obtaining yields of 0.97% and 0.95% (w/w) of yellowish oils, respectively. In the essential oils of this species, 27 and 25 components were identified representing 92.60% and 94.26% of the oils, respectively. S. viridis has 30 components (0.78 w/w). The essential oils of S.viridis have 29 components and S. syriaca was identified with 31 components. Additionally, this species was representing 91.54% and 91.02% of the oils, respectively.
Based on other research that has been published, multivariate analysis was employed [43]. The chemicals for the various samples were identified using principal component analysis (PCA) and cluster analysis (CA). The PCA was then carried out using the matrix correlation setup and Varimax rotation. PC1 (48.13%) and PC2 (10.04%) were the primary components in the principal component analysis. The total load of PC1 and PC2 was 58.17%. The Kaiser-Meyer-Olkin (KMO) approach was used to investigate the correlation of the variables. KMO was 0.613, which is considered satisfactory. Barlett's test of sphericity indicated statistical significance at alpha 0.06 for the data set. PCA analysis was explained in two ways, which revealed the link between the six Salvia species and their essential oil concentration (Figure 1).

Antimicrobial Activity Studies
In this step of the study, the antimicrobial activity of the essential oil obtained from the above-ground parts of six species belonging to the genus Salvia was tested by the disc diffusion method. Antimicrobial activity was tested against E. coli, K. pneumoniae, B. megaterium, S. aureus bacteria, C. albicans, and C. glabrata yeasts. Streptomycin sulphate 10 µg/disc for bacteria and Nystatin 100 µg/disc for yeasts were used as controls.
Essential oils of Salvia species showed varying antimicrobial activity against the microorganisms studied. The highest antimicrobial effect against E. coli was observed in S. multicaulis (25 mm), while the lowest antimicrobial effect was observed in S. absconditiflora (19 mm). The highest antimicrobial effect against K. pneumoniae was observed in S. verbenaca (25 mm), while the lowest antimicrobial effect was observed in S. syriaca (13 mm). The highest antimicrobial effect against B. megaterium was observed in S. multicaulis (28 mm), while the lowest antimicrobial effect was observed in S. absconditiflora (22 mm). The highest antimicrobial effect against S. aureus was observed in S. ceratophylla (22 mm), while the lowest antimicrobial effect was observed in S. viridis (10 mm). The highest antimicrobial effect against C. albicans was observed in S. verbenaca (28 mm), while the lowest antimicrobial effect was observed in S. syriaca (15 mm). The highest antimicrobial effect against C. glabrata was observed in S. multicaulis (25 mm), while the lowest antimicrobial effect was observed in S. syriaca (10 mm). According to these results, S. multicaulis and S. verbenaca species had the strongest antimicrobial activity, while S. absconditiflora and S. syriaca had the lowest activity. The antimicrobial activity of the plant samples against the test microorganisms is shown graphically in Figure 2.

Morphology Properties
Morphological observations and measurements of the studied Salvia species were made from herbarium specimens. Stem lengths, leaf measurements and characters, calyx and corolla characteristics and measurements, petiole measurements, inflorescence types, and hair conditions of the studied six Salvia species were determined. The endemic species S. absconditiflora was a perennial herb with elliptical cordate leaves, whose habitats were roadsides, uncultivated fields, slopes, and rocky limestone. The habitat of S. ceratophylla is mud and inactive, limestone rocky areas. The stem of this biennial species is erect and strong and has dense glandular hairs. The two perennial species S. multicaulis and S. verbenaca are very similar to each other (Figure 7), but differ in that S. verbenaca is densely hairy. S. multicaulis have hair on the body pilose to villous, rarely glabrous, sometimes dendroid hairy. S. viridis was an annual plant and its habitats were rocky slopes. S. syriaca was a perennial herb, rhizomatous. The stem was upright branches, and glandular feathers are quite dense. Descriptions of morphological and morphometric characters are described in Table 4, Figure 7 on the six Salvia species. All of the morphological measurements were performed using hierarchical clustering analysis and the resulting dendrogram was shown in Figure 7. Two large clusters were formed as a result of clustering analysis. S. absconditiflora, S. multicaulis, and S. verbenaca species are located on one side of the cluster (Figure 7). S. multicaulis, which is in the outermost clade, and S. absconditiflora, which is the closest to it, are species located in the same section in the Flora of Turkey [6] and can be distinguished morphologically by their leaf sizes and the color status of the calyx. The cluster tree in this study confirms these results in terms of morphology in Figure 8.

Anatomical Properties
The stem epidermis of S. absconditiflora has a layer of collenchyma embedded in the cortex, usually below a single-layered epidermis. In section through the stem, the pith covered a large area. In the stem cross-section, most of the cells in the periderm were crushed. Xylem rails were obvious. In the cross-section of the petiole of S. absconditiflora, there were two areas named abaxial surface and adaxial surface. The adaxial surface has a convex shape. A cuticle surrounded the petiole. A single row of rectangular and oval cells made up the epidermis. The epidermis was covered with trichomes. The stem of S. ceratophylla have located single-layered and made up of cells with an oval-oblong shape. Sclerenchymatic cells consisted of 3-7 layers and were usually stained red color on sections. The root of S. ceratophylla a periderm located at the outermost part was dark-colored. Most of the periderm was crushed and its cell structure was disrupted. In the petiole's crosssection of this species, the adaxial surface was convex. The stem epidermis of S. multicaulis has a single-layered epidermis and is made up of cells that were typically oval-oblong and sometimes square-like in shape.
The stem epidermis of S. verbenaca consisted of oval-oblong, sometimes square-like cells and was single-layered. There was a layer of collenchyma in the cortex under the epidermis in certain spots, and both adaxial and abaxial surfaces were convex and twisted in a petiole cross-section. The stem epidermis of S. viridis and S. syriaca were single-layered and consisted of mostly ovoidal rectangular. When we took a cross-section of the petiole S. viridis, the epidermis consisted of a single row of rectangular and oval cells. The epidermis was covered with trichomes. The petiole's cross-section of S. syriaca has an abaxial surface and the adaxial surface were procumbent and D-shaped. Petiole was covered with a cuticle. The epidermis consisted of a single row of rectangular and oval cells. The epidermis was covered with trichomes. Descriptions of anatomical characters are expanded with the detailed investigations on six Salvia species in Table 5 and Figures 8 and 9.

Palynological Properties
All morphological parameters determined have been shown in Table 3 and Figures 3-6. Under LM, the pollen grains of the 6 belonging to Salvia were isopolar and radially symmetrical. The pollen was symmetrical relative to the equatorial diameter.
S. absconditiflora, S. ceratophylla, and S. viridis species are closest to each other in cluster analysis in Figure 4. S. multicaulis species is the most in the outermost clade. A P/E ratio of 1.03 prolate-spheroidal has the largest pollen in this study. This cluster analysis has been carried out correctly to provide this data.

Discussion
The essential oil constituents and antimicrobial properties as well as some biosystematic characteristics (morphological, palynological, and anatomical features) of Salvia samples from different regions of Turkey were studied qualitatively and quantitatively.
In morphological examinations, calyx and corolla shape, bract structure, and inflorescence status are important characters in determining the species included in the six Salvia species. No new characters other than those described in the literature concerning the morphological traits of the species that served as the focus of this study were discovered. It was observed that the morphological measurement values of the samples belonging to the Salvia species were in great agreement with the findings of the literature [6], as well as some deviations in the minimum and maximum limits of the measurement values. For example, when morphologically examined, the bracts and leaves of the S. viridis species were measured smaller than the Flora of Turkey [6] in this study. S. multicaulis stem length was measured as smaller and leaves were larger in this study when compared to the Flora of Turkey. The species we collected were taken from a higher altitude compared to the Flora of Turkey. These differences are also observed in Figure 4 and the morphological distinction of the species from each other is indicated in the cluster analysis. When the morphological measurements were compared with the literature, the reason for these differences can be attributed to the difference in the number of samples examined and the place and time of collection. Baran reported that leaf size was 1.2-6 × 0.6-2.8 cm and the corolla size was 0.9-1.5 cm in Salvia viridis [44]. The findings obtained in this study showed that the leaves were 1.5-2.5 × 1-3 cm in size and simple, oblong-ovate; corolla size 8-10 mm.
In this study, 1,8-cineole, which is highly present in Salvia essential oils, is used as a component of many medicines such as antiseptics, nasal sprays, mouthwashes, cough syrups, medicated lozenges, and as an additive in personal care products such as toothpaste and aromatherapy oils. Due to the pleasant flavor and aroma of the compound, it is used as a sweetener in products such as confectionery, pastry, bakery products, beverages, and meat products [52]. In a study, the efficacy of 1,8-cineole on the antimicrobial effect against some microorganisms was investigated. As a result of the study, it was concluded that the use of 1,8-cineole in combination with chlorhexidine may facilitate the elimination of some resistant bacteria by increasing antimicrobial activity [53]. The primary sesquiterpene in hops, caryophyllene, or its derivatives, are used in soaps and scents for cosmetic purposes [54]. Hops' modest sedative effects in herbal medicine are caused by the compound caryophyllene. Furthermore, investigations conducted in vitro showed that caryophyllene has lethal effects on breast cancer cells [55]. The caryophyllene oxide levels in this research showed that as follows; S. absconditiflora 10.14%, S. ceratophylla 14.68%, S. multicaulis 17.20%, S. verbenaca 16.15%, S. viridis 16.18%, and S. syriaca 17.54% ( Table 2). The detailed oil composition characterization carried out in this study revealed the presence of various valuable compounds in the chosen Salvia species demonstrating their applicability for medicinal and pharmaceutical purposes as well as in the cosmetic beverages industry.
Spathulenol, which was determined as the major compound in the study, is a sesquiterpene component found in essential oils. It has been reported to play a major role in antimicrobial, antiproliferative, anti-inflammatory, and immunomodulatory activities [56,57]. It was also found to have a repellent effect against mosquito species [58]. According to the results of this study, all studied species showed high amounts of spathulenol. Borneol, the other major component, is a colthisless, crystalline monoterpene occurring in essential oils. Borneol has been proven to have antibacterial, antifungal, antispasmodic, choleretic, and sedative effects [59,60]. Recent studies have shown that the blood-brain barrier improves drug delivery and increases efficacy [61]. At the same time, it was determined that borneol showed antiapoptotic, antioxidative, and neuroprotective effects in human neuroblastoma cells [62].
The biochemical contents of Salvia species, the solvents used and the differences of microorganisms affect the antimicrobial results. This study reveals that the antimicrobial effect of Salvia essential oils is very important. In a previous study, the ethanol extract of the species S. absconditiflora (S. cryptantha) was tested by the disk diffusion method. As a result of the study, the antimicrobial effect of the plant extracts against "gram+" bacteria was found, while the same effect against "gram-" bacteria and C. albicans yeast was not found [63]. In this study, the essential oil of S. absconditiflora was effective against both "gram+" and "gram-" bacteria. In an antimicrobial study of S. ceratophylla extract, it was observed that it showed a strong antimicrobial effect [64]. Previous studies reported that the essential oils of S. multicaulis were effective against S. aureus, K. pneumoniae, E. coli, and Streptococcus mutants [65]. In another study, the essential oils of S. multicaulis were found to be effective against Bacillus sp., Enterococcus sp., Staphylococcus sp., and Saccharomyces cerevisiae [66,67]. In another study, disc diffusion of the essential oil of S. verbenaca species showed antimicrobial activity against Bacillus sp. and Staphylococcus sp. [68]. The essential oils obtained in this study were included in antimicrobial activity studies by the disk diffusion method. This is the first study on the antimicrobial activity of S. absconditiflora, S. ceratophylla, and S. viridis species using this method. In the present study, it was determined that the six Salvia species could be considered as a natural antimicrobial source against the tested microorganisms.
In an anatomical study on S. forskaohlei L., it was determined that there was a sclerenchymatous ring with sclerenchyma clusters under the parenchymatic cortex cells in the root of S. forskaohlei [69]. Çobanoglu mentioned these sclerenchyma clusters in the root cortex of the species in his study on S. palestina Bentham [70]. These findings showed that, in anatomical examinations, sclerenchyma clusters in the root cortex of the species were found in S. ceratophylla and S. multicaulis species and not in other species.
Metcalfe and Chalk [71] stated that the typical feature of the family is the presence of a well-developed collenchyma tissue at the corners of the stem. Thickening of the collenchyma tissue was observed and photographed in the examined Salvia species. Kahraman reported that S. absconditiflora, S. viridis, S. ceratophylla, S. syriaca, and S. viridis, had a very large cortex and the epidermis consisting of a single subcaste of nearly rectangular, square, or round cells [72]. In this study, a large cortex was observed in the stem sections of the species. In addition, the shape of the epidermis was observed in the cross-sections of the stem in this study, usually ovoidal rectangular and sometimes square. In his study, Kahraman was able to categorize the petiole anatomy of Salvia species in a cross-section into seven types. He reported that U-shaped with obtuse or erect margins (S. viridis), D-shaped with more or less procumbent margins (S. syriaca), triangular (S. absconditiflora, S. multicaulis) or open crescent-shaped.
Özler et al. pronounced that the Salvia section's pollen suboblate to subprolate and aperture circumstance is hexacolpate and octacolpate [73]. In another study, Özler et al. pronounced that the S. multicaulis pollen grain is prolate spheroidal. In this study of the Hymenosphace section, S. absconditiflora pollen grain is prolate spheroidal, and S. multicaulis pollen grain is suboblate [73,74].
The findings obtained in this study showed that S. syriaca, S. verbenaca, and S. ceratophylla in the Aethiopis section species pollen are subprolate, subprolate, and suboblate, respectively. Kiliç reported that S. syriaca pollen is suboblate [75]. Moon et al. [76] reported bireticulate ornamentation in pollen of the Aethiopis section, and another study discovered that S. syriaca was characterized by reticulate-perforate [73,74]. In this study, the S. viridis pollen grain in the Horminum section is oblate-spheroidal. When this study is evaluated regarding palynological results, it was concluded that pollen morphology characteristics of species were generally similar to each other. Pollen morphological characteristics were not distinguishable in taxonomy in the identification of Salvia species observed in this study since there was no discernible variation in the palynological characteristics of the taxa analyzed. This view is supported by some other studies [73][74][75][76].

Conclusions
The chemotaxonomic study showed that the essential oil of Salvia species varies slightly depending on ecological, climatic, plant collection periods, and location. However, it is also a fact that the amounts of major common constituents in Salvia species vary depending on the species of the species. In other words, the fact that the constituents in Salvia species are generally similar, without the effect of harvest time and locality, makes it possible to standardize the essential oils of Salvia species. The detailed characterization of oil composition carried out in this study has revealed the existence of various valuable compounds in the selected Salvia species, demonstrating their applicability for medicinal and pharmaceutical purposes as well as in the cosmetic and beverage industries. According to the experimental results, it was found to have antimicrobial activity against all tested microorganisms at certain rates. It is believed that the strong antimicrobial effect is due to these valuable chemical components. New research should be carried out on other Salvia species and in new areas, and new data should be obtained by conducting practical experiments on the antimicrobial and antibacterial effects of Salvia species on rats.
Additionally, the morphological, anatomical, and palynological information gleaned from this research will serve as a foundation for biosystematic analyses of Salvia species.

Conflicts of Interest:
The author declares no potential conflict of interest regarding publication of this research work.