Microangiopathy in Rheumatic Diseases

Capillaries are part of the microcirculation, which consists of arterioles, capillaries, and venules and are the connecting link between the arterial and venous blood circulation [...].

Life 2023, 13, 491 2 of 5 of a few giant capillaries; a few microhemorrhages but with preserved capillary density and architecture. In the "active" phase there are frequent giant capillary loops and microhemorrhages associated with moderate devascularization and capillary derangement. Finally, in the "late" phase capillary loss and capillary derangement are severe and there are newly formed, neoangiogenic capillaries, while microhemorrhages are absent and giant capillaries are single or absent [23]. Validated staging for "scleroderma-like" capillaroscopic changes has not been established yet; however, it has been observed that, in some cases, "scleroderma-like" capillaroscopic findings are indistinguishable from those in SSc, while in other cases the presence of discriminating features has been suggested that are to be precisely characterized [24].
Previously, it was considered that "scleroderma-like" capillaroscopic changes in SLE are associated with anti-RNP antibodies and indicate subclinical overlap with SSc [19,25], while there is now increasing evidence that "scleroderma-like" pattern could be observed in SLE patients without features of overlap and without association with anti-RNP antibodies [12][13][14][15]. In an own study (2013), a "scleroderma-like" pattern has been observed in 13.3% of examined SLE patients without signs of overlap syndrome with SSc and without association with anti-RNP antibodies. This finding was detected in patients with symptoms of secondary RP, and in half of the cases the vasculitis of digital vessels was present [12]. These observations were also later confirmed in other patient populations (van Roon et al., 2019; frequency of 17%) [13], including in children with SLE [14,15]. Of note, an association between "scleroderma-like" microangiopathy and cutaneous digital lesions in patients with both SLE and cutaneous lupus erythematosus with digital skin involvement was reported [12,26,27]. Nail fold capillaroscopy has also been suggested to play a valuable role in the evaluation of peripheral microcirculation in RA [20,28]. Initially, it was considered that "scleroderma-like" microangiopathy could not be observed in RA [17,29]. In an own study (2012), "scleroderma-like" capillaroscopic changes have been found in 14.5% of examined RA patients and by analogy with SLE, such changes were present in cases with digital vasculitis or secondary RP but without signs of overlap syndrome [20]. Later, Rajaei et al. (2017) also confirmed the presence of "scleroderma-like" capillaroscopic pattern in RA patients (frequency of 20.9%) without the presence of overlaps [21].
Capillaroscopic examination is a key method that supports the early diagnosis of SSc and other rheumatic diseases with peripheral vascular syndrome. It is a mandatory diagnostic tool in all patients with RP together with ANA screening test and if indicated in a clinical context-a test for disease-specific antibodies. The existence of an association between microangiopathy and autoantibodies (diagnostic and pathogenic) is a question that is insufficiently studied in contemporary rheumatology. In SSc, anti-Scl70 antibody positivity is suggested to be related to the earlier appearance of more advanced microvascular changes (i.e., "late" capillaroscopic patterns) [30][31][32]. In an own study, it has been found, as a novel observation, that positive anti-RNA polymerase III-155 antibodies might be detected in SSc patients without microvascular pathology in the nail fold area or with early microangiopathy [32]. These data suggest the presence of a link between immunological and microvascular disturbances and require further research for both diagnostic and pathogenic antibodies.
Among rheumatic diseases, microangiopathy is not only the most prevalent in SSc, but also in scleroderma patients it is characterized with the greatest severity and extent. Moreover, vascular damage is suggested to be the initial pathological event of the disease that subsequently triggers inflammation and fibrosis. This notion underlines the high value of microvascular assessment in the initial stages of the disease. Endothelial injury in SSc that is initiated by an unknown agent, i.e., free oxygen radical, microbes, antibodies, chemicals, is characterized by the development of dysbalance between the levels of vasodilators (nitric oxide, prostacyclin, etc.) and vasoconstrictors (endothelin, angiotensin, etc.), with the prevalence of vasoconstrictors. Damaged endothelial cells of the microcirculation mediate the proliferation and contraction of smooth muscle cells, promote the formation of microthrombi due to the impairment of the antithrombotic function of the endothelium and lead to reduction in fibrinolysis. There is overexpression and release of adhesion molecules that trigger inflammation. These pathological processes lead to tissue hypoxia, inflammation, and subsequent fibrosis [33][34][35][36]. Disturbances in vascular recovery are also characteristic for SSc, which affect both the processes of angiogenesis (new blood vessel formation from the endothelial cells of pre-existing vessels) and vasculogenesis (formation of new vessels from the endothelial progenitor cells derived from bone marrow) [37][38][39][40].
Due to the profound endothelial damage, tissue hypoxia, defective angio-and vasculogenesis, and the accompanying fibrotic process in SSc, ulcerations with different localizations are frequent findings. Digital ulcers are among the most common type of ulcerations in SSc, which include ischemic lesions at the finger pads, digital ulcers over bony prominences, and over calcifications, digital necrotic lesions. Digital ulcers in SSc require a complex therapeutic approach that includes the administration of vasodilators, local antiseptic treatment, antiplatelet drugs, anticoagulants, and antibiotics in some cases [41]. However, due to the presence of generalized microangiopathy in SSc, non-digital ulcerations can be observed at different body areas that are less well-studied. They may develop over bony prominences, over calcinosis, and on lower extremities [42]. Their etiology is complex and includes microvascular damage at the levels of the skin, tendons, fascia [42,43], and disturbances in tissue repair, i.e., the reduced proliferative potential of mesenchymal stem cells, defective circulating endothelial progenitor cells with ineffective vasculogenesis that results in impaired wound healing, development of chronic skin ulcers [42], and compromised physical capacity [43]. Thus, the treatment of ulcerations in SSc is a significant challenge in clinical practice. Novel therapeutic strategies have been suggested, such as local administration of mesenchymal stem cells derived from bone marrow or adipose tissue and initial promising results have been reported in patients with chronic skin ulcers resistant to treatment, including in SSc cases [44,45]. In a recent paper of Biehl et al. (2022), a new approach to ulcerations of lower extremities associated with calcinosis in SSc has been presented that includes the surgical removal of calcifications, vacuum therapy for the improvement of vascularization, and tissue perfusion by stimulating neoangiogenesis, lymphatic drainage, and intensified physiotherapy [43]. Further observations of the therapeutic efficacy of these new strategies are awaited to improve the complex care of SSc patients with chronic skin ulcers resistant to treatment.
In conclusion, microvascular damage plays a key role in a number of rheumatic diseases. In SSc and CTD associated with peripheral vascular syndrome, microvascular pathology is readily assessed in the nail fold area that facilitates the early diagnosis. A better understanding of the different aspects of microvascular abnormalities in rheumatic diseases might improve the diagnosis of the early microangiopathy and expand the knowledge of disease pathogenesis.

Conflicts of Interest:
The author declares no conflict of interest.