Screening of Bioactive Compounds from Endophytic Marine-Derived Fungi in Saudi Arabia: Antimicrobial and Anticancer Potential

Nowadays, endophytic fungi represent a rich source of biological active compounds. In the current study, twelve endophytic fungal species were isolated from Avicennia marina leaves. From the isolates, Aspergillus niger, Penicillium rubens and Alternaria alternata recorded the highest isolation frequency (80%), relative density (12.5%) and antimicrobial activity. The antimicrobial and anticancer activities of P. rubens were more effective than those of A. niger and A. alternata; therefore, its identification was confirmed via the ITS rRNA gene. Filtrate extracts of P. rubens, A. alternata and A. niger were analyzed using GC-MS and showed different detected constituents, such as acetic acid ethyl ester, N-(4,6-Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide, 1,2-benzenedicarboxylic acid, hexadecanoic acid and octadecanoic acid. Filtrate extract of P. rubens exhibited the presence of more compounds than A. alternata and A. niger. Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Aspergillus fumigatus were more inhibited by P. rubens extract than A. alternata or A. niger, with inhibition zones of 27.2 mm, 22.21 mm, 26.26 mm, 27.33 mm, 28.25 mm and 8.5 mm, respectively. We observed negligible cytotoxicity of P. rubens extract against normal cells of human lung fibroblasts (WI-38 cell line), unlike A. alternata and A. niger extracts. Proliferation of prostate cancer (PC-3) was inhibited using P. rubens extract, exhibiting mortality levels of 75.91% and 76.2% at 200 µg/mL and 400 µg/mL of the extract. Molecular docking studies against the crystal structures of C. albicans (6TZ6) and the cryo-EM structure of B. subtilis (7CKQ) showed significant interactions with benzenedicarboxylic acid and N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide as a constituent of P. rubens extract. N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide had the highest scores of −6.04905 kcal/mol and −6.590 kcal/mol towards (6tz6) and (7CKQ), respectively.


Introduction
Microorganisms, particularly endophytes isolated from diverse marine habitats, have been extensively studied because they are considered a rich resource for pharmaceuticals

Source of Endophytic Fungi
Endophytic fungi were isolated from mangrove plants (Avicennia marina) that were propagated in the Jazan region, Saudi Arabia, situated along the Red Sea, located at 16 • 47 00.7 N latitude and 42 • 40 30.8 E.

Endophytic Fungi Isolation and Cultivation
The collected plant leaves of A. marina were washed several times (5 times) using running tap water to remove any debris such as dust or the waste of birds, and then dipped in ethanol (70%) for 1-2 min. Under aseptic conditions, the leaves were ruptured into small segments (2 mm) and then dipped in NaOCl (4%) for 1 min. The small segments were rinsed in sterile distilled water 5 times, followed by drying via sterile filter paper [20]. The dried segments were placed on fungal growth agar medium plates that contained 15 g/L of malt extract, 10 g/L of artificial sea salt, 20 g/L of bacto agar, 1 L of sterile distilled water and 0.2 g/L of chloramphenicol to prevent bacterial growth. Medium pH was adjusted to 7.7 according to the pH analysis of sea water. At 30 • C, the plates were incubated for 8 days. The appeared fungal hyphae tips from the segments of leaves were sub-cultured several times on the same growth medium to obtain pure fungal colonies.
The isolation frequency (IF) and relative density (Rd) of species were recorded as follows:

Identification of Fungal Isolates
To identify the fungal isolates, the pure fungal cultures were re-cultivated on different media (potato dextrose agar, malt extract and Czapek Dox agar were provided by Sigma-Aldrich, St. Louis, MO, USA) to assess the growth rate, colony color and form, margin characteristics and elevation. At the same time, the fungal colonies were microscopically examined to visualize the shape and diameters of fungal mycelia, conidiophores, conidiospores, chlamydospores, sexual spores and fruiting bodies [21][22][23][24][25]. The fungus' potent antimicrobial activity was identified molecularly as follows. DNA was extracted from 100 mg of fungus mycelia (collected from fungus growing on broth medium). The 18S ribosomal DNA (rDNA) internal transcribed spacer (ITS) region was amplified via polymerase chain reaction (PCR) using the Quick-DNA Fungal Microprep Kit (Zymo research; D6007; Irvine, CA, USA) with the following primers: ITS1-forward (5 -TCCGTAGGTGAACCTGCGG-3 ) ITS4-reverse (5 -TCCTCCGCTTATTGATATGC-3 ) [26,27], at Sigma Scientific Services Company, Cairo, Egypt. The total volume of PCR reaction containing the extracted genomic DNA (10 µL) included 1 µL of each primer (forward primer 18S rRNA and reverse primer 18S rRNA) and 1 µL of nuclease, and 25 µL of Maxima ® 2× Hot Start PCR Master Mix was prepared to perform the reaction of PCR. PCR amplification was performed at the following temperature cycles: a first step of 2 min at 94 • C for the first initial step, followed by 40 cycles at 94 • C for 60 s, then at 52 • C for 90 s, and for 2 min at 72 • C, and a final cycle at 72 • for 7 min.

Preparation of Marine Fungal Extract
After the incubation period, 100 mL of the metabolized medium free of fungal mycelia was filtrated via filter paper (Whatman No. 1 filter paper). At low pressure (8 × 10 3 Pa), the fungal filtrate was evaporated to obtain the extract, which was subsequently extracted with methanol and then concentrated to obtain a crude extract for further study, including gas chromatography-mass spectrometry (GC-MS) analysis and biological activities.

GC-MS Analysis of Fungal Filtrate Extract
The fungal extract was analyzed using GC (THERMO Scientific Corp., Waltham, MA, USA) joined with MS (ISQ Single Quadrupole Mass Spectrometer, Waltham, MA, USA). Fungal extract (1 µL) was injected into the chromatography column (30 m × 0.32 mm × 0.25 µm) via the Autosampler AS1300. Temperature cycling was programmed at 60 • C for initial analysis, and then increased to 240 • C. Subsequently, it was increased gradually to 290 • C for 2 min. Helium with the highest purity was utilized as a carrier at a rate of 1 mL/min. The electron ionization mass of the spectra was collected in full scan mode at a range of m/z 40-1000 via electron energy of 70 eV as desired. Then, via calculation of the retention time (RT) and mass spectra of the discovered compounds, they were identified by comparison to the mass spectra logged at the library of the National Institute of Standards and Technology [28].

Disk Diffusion Method and MIC Detection for Antimicrobial Activity of Fungal Extract
The antimicrobial activity of the fungal extract was assayed against 4 species of bacteria, including Bacillus subtilis, Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa (provided by Hospitals of Ain Shams University in Egypt), as a source of the tested fungi and two fungi including Candida albicans and Aspergillus fumigatus (provided by Assiut University Mycological Centre in Egypt). A disk of filter paper (6 mm) was loaded with 100 µL of fungal filtrate extract and then pasted on a nutrient agar medium surface inoculated with tested bacteria, while potato dextrose agar medium was used for tested fungi. Then, the plates were kept in a refrigerator at 3 • C for 20 min for suitable extract diffusion. After this, the plates were transferred to an incubator (adjusted at 28 • C for 3 days required for fungi and 37 • C for 24 h required for bacterial growth). The visualized clear zone around the disc loaded with fungal filtrate extract was measured (mm). Control discs were loaded with antibiotic gentamycin as an antibacterial agent and ketoconazole as an antifungal agent [29]. A microdilution protocol was applied to determine the minimum inhibitory concentration (MIC) of fungal filtrate extract. Different dilutions of the extract that were dissolved in dimethylsulfoxide (DMSO) were prepared through the production of serial dilutions. Suspensions of bacteria and fungi inocula were prepared (2 × 10 8 and 2 × 10 6 colony-forming units/mL of bacteria and fungi, respectively) and inoculated (100 µL) in macro-dilution tubes containing the fungal filtrate extract. At 37 • C for 18 h (required for bacteria) or for 24 h (required for yeast), the macro-dilution tubes were incubated, followed by measuring the OD at 600 nm.

Cytotoxicity Assay against Cancer Cells
The used culture cells were prostate cancer cells (PC-3) that were provided by Nawah Scientific Inc. (Mokatam, Cairo, Egypt). DMEM medium, amended with streptomycin (100 mg/mL), penicillin (100 units/mL) and heat-inactivated fetal bovine serum (10%) and humidified, was used to maintain the cells under CO 2 (5% v/v) and at 37 • C. The SRB assay was applied to determine the effect of the fungal extract on the PC-3 viability. Suspensions (100 µL) of PC-3 containing 5 × 10 3 cells were placed in 96-well plates, followed by incubation for 24 h. Different concentrations of the fungal extract were added to cell medium; 100 µL of medium containing the extract was added to the cultivated cells and incubated for 3 days. Then, the treated cells were fixed via 150 µL of 10% TCA instead of media, followed by incubation for 1 h at 4 • C. After this, the solution of TCA was removed, followed by washing the cells using distilled water 5 times. Aliquots of 70 µL of 0.4% w/v SRB solution were added to PC-3, and then incubated for 10 min in a dark area at 25 • C. With 1% acetic acid, the plates were washed 4 times and then allowed to dry in air for 12 h; subsequently, 150 µL of TRIS (10 mM), required to dissolve the protein-bound SRB stain, was added; at this point, using the BMG LABTECH ® -FLUOstar Omega microplate reader (Ortenberg, Germany), the absorbance was recorded at 540 nm [30].

Cytotoxicity Assay against Normal Cells
The used culture cells were the WI-38 cell line (human lung fibroblast normal cells) that were provided by Nawah Scientific Inc. (Mokatam, Cairo, Egypt). Cells were propagated in Dulbecco's modified Eagle's medium (DMEM) amended with 10% fetal bovine serum (FBS) at 37 • C in a humidified incubator (5% CO 2 ). A suspension (100 µL) of the WI-38 cell line (5 × 10 3 cell/mL) was plated in microtiter plates (96-well), followed by incubation for 18 h. Subsequently, different doses of filtrate fungal extract were loaded onto each used well and the incubation period was completed, lasting 2 days. To each well, the prepared solution (20 µL) of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (5 mg/mL) was added, followed by incubation for 4 h. The absorbance was recorded at 540 nm to estimate the cytotoxicity activity [30].

Molecular Docking Studies with MOE
As a structural biology method, molecular docking was used to evaluate the interactions between pairs of molecules, typically a receptor and a ligand. Molecular docking modeling was performed using the Molecular Modeling Environment (MOE) (MOE 2019.0102 program). The crystal structure of C. albicans (6TZ6) and the cryo-EM structure of B. subtilis (7CKQ) were downloaded from the Protein Data Bank (https://www.rcsb.org/pdb/ (accessed on 19 July 2022)). The native compounds were prepared prior to the docking experiment by minimizing their energy at the B3LYP/631G (d) level of theory. Partial charges were calculated by Gasteiger's method. The last structure was acquired after 3D protonation and the correction process. The bound water, ligands and cofactors were removed from the protein. The MOE site finder generated the active binding sites to create the dummy sites as the binding pocket. Using the triangular matching docking method, the target molecules were docked into the protein's active site, and thirty conformations of the title compounds and protein complex were created with a docking score (S kcal/mol). The docking protocol predicted the best five conformations as present in the crystal structure with a better RMSD value. The complexes were analyzed for interactions and their 3D images were taken by using a visualizing tool, PyMol (The PyMOL Molecular Graphics System, Version 1.2r3pre, Schrödinger, LLC, New York, NY, USA).
The following definitions were used in the molecular docking studies: final score (S), which is the score of the last stage that was not set to zero; the root mean square deviation (rmsd) of the pose, in Å, from the original ligand-this field is present if the site definition was identical to the ligand definition; rmsd_refine, meaning the root mean square deviation among the pose before refinement and the pose after refinement; E_conf meaning the energy of the conformer-if there is a refinement stage, this is the energy estimated at the end of the refinement. Annotation was performed for force field refinement; by default, this energy is estimated with the solvation option set to Born. We also included E_place, meaning the score from the placement stage; E_score 1 and E_score 2, meaning the scores from rescoring stages 1 and 2; and E_refine, meaning the score from the refinement stage, estimated to be the whole of the van der Waals electrostatics and solvation energies under the generalized Born solvation model.

Statistical Analysis
All experimental results were realized in triplicate. The standard deviation (SD) and variance were calculated via SPSS ver. 22.0 software (version 14, IBM Corp., Armonk, NY, USA).

Endophytic Fungi Isolates
Thirty fungal isolates, belonging to twelve fungal species, were isolated from leaf segments of Avicennia marina collected from four sites (A, B, C and D) in the same region that was characterized by mangrove clumps (Figure 1A-D) in Jazan city, Saudi Arabia. The fungal isolates belonged to Aspergillus niger, Alternaria alternata, Exserohilum rostratum, Mucor racemosus, Acremonium sp., Penicillium rubens, Alternaria chlamydospora, Fusarium proliferatum, Aspergillus phoenicis, Penicillium chrysogenum, Cladosporium herbarum and Eurotium amstelodami with different IF (%) and Rd (%). A. niger, P. rubens and A. alternata occurred with the highest isolation frequency (80%) and relative density (12.5%), while other isolates showed the lowest IF (20%) and Rd (3.12%), specifically E. amstelodami and Acremonium sp. Our results were partially in line with the obtained results of Hamed et al. [32], who identified various endophyte fungi of marine habitats that belonged to eight different genera of Cladosporium, Epicoccum, Alternaria, Aspergillus, Byssochlamys, Talaromyces Penicillium and Sarocladium. The dominant species P. rubens, A. alternata and A. niger in the current study were identified to the level of species, also as mentioned by Hamed et al. [32]. Some previous reports indicated that the fungal diversity is influenced by the availability and sources of nutrients, as well as the physicochemical factors of the coastal ecosystem [33]. Therefore, in another study, Acremonium curvulum was the most frequently found endophyte (9.5%), followed by Aspergillus ochraceus, isolated from Bauhinia forficata [6]. The three fungal isolates with the highest isolation frequency ( Figure 2) were assessed for antimicrobial activity against different bacteria and fungi. The identification of the fungus with the highest antimicrobial activity was confirmed with molecular identification via the ITS rRNA gene. Through the alignment search tool available at https://blast.ncbi.nlm.nih.gov (accessed on 19 July 2022), the value of ITS rRNA homology for the isolated strain exhibited resemblance 99.12% and query cover 100% with P. rubens (OM836432.1) as existing in the phylogenetic tree ( Figure 3); the sequence was recorded in GenBank with accession number OM836434.1.
Life 2022, 12, x FOR PEER REVIEW 6 of 22 E_score 2, meaning the scores from rescoring stages 1 and 2; and E_refine, meaning the score from the refinement stage, estimated to be the whole of the van der Waals electrostatics and solvation energies under the generalized Born solvation model.

Statistical Analysis
All experimental results were realized in triplicate. The standard deviation (SD) and variance were calculated via SPSS ver. 22.0 software (version 14, IBM Corp., Armonk, NY, USA).

Endophytic Fungi Isolates
Thirty fungal isolates, belonging to twelve fungal species, were isolated from leaf segments of Avicennia marina collected from four sites (A, B, C and D) in the same region that was characterized by mangrove clumps ( Figure 1A-D) in Jazan city, Saudi Arabia. The fungal isolates belonged to Aspergillus niger, Alternaria alternata, Exserohilum rostratum, Mucor racemosus, Acremonium sp., Penicillium rubens, Alternaria chlamydospora, Fusarium proliferatum, Aspergillus phoenicis, Penicillium chrysogenum, Cladosporium herbarum and Eurotium amstelodami with different IF (%) and Rd (%). A. niger, P. rubens and A. alternata occurred with the highest isolation frequency (80%) and relative density (12.5%), while other isolates showed the lowest IF (20%) and Rd (3.12%), specifically E. amstelodami and Acremonium sp. Our results were partially in line with the obtained results of Hamed et al. [32], who identified various endophyte fungi of marine habitats that belonged to eight different genera of Cladosporium, Epicoccum, Alternaria, Aspergillus, Byssochlamys, Talaromyces Penicillium and Sarocladium. The dominant species P. rubens, A. alternata and A. niger in the current study were identified to the level of species, also as mentioned by Hamed et al. [32]. Some previous reports indicated that the fungal diversity is influenced by the availability and sources of nutrients, as well as the physicochemical factors of the coastal ecosystem [33]. Therefore, in another study, Acremonium curvulum was the most frequently found endophyte (9.5%), followed by Aspergillus ochraceus, isolated from Bauhinia forficata [6]. The three fungal isolates with the highest isolation frequency ( Figure 2) were assessed for antimicrobial activity against different bacteria and fungi. The identification of the fungus with the highest antimicrobial activity was confirmed with molecular identification via the ITS rRNA gene. Through the alignment search tool available at https://blast.ncbi.nlm.nih.gov, the value of ITS rRNA homology for the isolated strain exhibited resemblance 99.12% and query cover 100% with P. rubens (OM836432.1) as existing in the phylogenetic tree (

GC-MS Analysis of Fungal Extract
GC-MS analysis of P. rubens, A. alternata and A. niger growth media extracts showed the existence of 16 (Table 1 and Figure 4), 9 (Table 2 and Figure 5) and 10 (Table 3 and Figure 6) compounds with different retention times and areas (%). Acetic acid ethyl ester, benzenesulfonamide, 1,2-benzenedicarboxylic acid, hexadecanoic acid and octadecanoic acid were detected in the filtrate extracts of the three fungal isolates. On the other hand, some compounds, such as tetradecanoic acid, tributyl acetylcitrate and propiolic acid, 3-(1-hydroxy-2-isopropyl-5-methylc yclohexyl)-, ethyl ester, were identified in the filtrate extract of P. rubens only. At the same time, oxiraneundecanoic acid, 3-pentyl-, methyl ester, cis and 9,12-octadecadienoyl chloride, (Z,Z) were identified in the filtrate extract of A. alternata. Secondary metabolites, which are created by marine-derived fungi, are potential sources of unique bioactive compounds. Multiple studies have focused on the isolation of endophytic fungi from diverse geographical and environmental regions to evaluate their productivity of various compounds with multiple uses in medicinal applications, such as anticancer, antiviral, antimicrobial and antioxidant activities [34][35][36].

GC-MS Analysis of Fungal Extract
GC-MS analysis of P. rubens, A. alternata and A. niger growth media extracts showed the existence of 16 (Table 1 and Figure 4), 9 (Table 2 and Figure 5) and 10 (Table 3 and Figure 6) compounds with different retention times and areas (%). Acetic acid ethyl ester, N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide, 1,2-benzenedicarboxylic acid, hexadecanoic acid and octadecanoic acid were detected in the filtrate extracts of the three fungal isolates. On the other hand, some compounds, such as tetradecanoic acid, tributyl acetylcitrate and propiolic acid, 3-(1-hydroxy-2-isopropyl-5methylc yclohexyl)-, ethyl ester, were identified in the filtrate extract of P. rubens only. At the same time, oxiraneundecanoic acid, 3-pentyl-, methyl ester, cis and 9,12-octadecadienoyl chloride, (Z,Z) were identified in the filtrate extract of A. alternata. Secondary metabolites, which are created by marine-derived fungi, are potential sources of unique bioactive compounds. Multiple studies have focused on the isolation of endophytic fungi from diverse geographical and environmental regions to evaluate their productivity of various compounds with multiple uses in medicinal applications, such as anticancer, antiviral, antimicrobial and antioxidant activities [34][35][36]. According to some studies, antimicrobial, antioxidant, anticancer and anti-inflammatory activities were attributed to the compoundsor their derivatives-that were found in the current study, such as n-hexadecanoic acid, 9,12-octadecandionoic acid, 11-octadecenoic acid and methyl ester [37]. Methyl stearate, which was identified in P. rubens extract (Table 1), has been applied as an antifungal, intestinal lipid regulating, nematicidal and anti-inflammatory agent [38]. Hexadecanoic acid and methyl ester (area 16.41%), found in the current study, previously exhibited antibacterial activity against definite Gr + ve and Gr − ve bacteria [39]. Results of GC-MS analysis of P. crustosum filtrate extract revealed that hexadecanoic acid was the main detected constituent [1]. The antibacterial and antioxidant activities of hexadecanoic acid have been documented [1,40].

Antimicrobial Activity of Fungal Extract
Antimicrobial activity of P. rubens, A. alternata and A. niger filtrate extracts was recorded against various microorganisms, but with different levels of growth inhibition (Table 4 and Figure 7). The highest activity was visualized using P. rubens filtrate extract

Antimicrobial Activity of Fungal Extract
Antimicrobial activity of P. rubens, A. alternata and A. niger filtrate extracts was recorded against various microorganisms, but with different levels of growth inhibition (Table 4 and Figure 7). The highest activity was visualized using P. rubens filtrate extract

Antimicrobial Activity of Fungal Extract
Antimicrobial activity of P. rubens, A. alternata and A. niger filtrate extracts was recorded against various microorganisms, but with different levels of growth inhibition (Table 4 and Figure 7). The highest activity was visualized using P. rubens filtrate extract against all tested bacteria, namely B. subtilis (inhibition zone was 27.2 mm), S.aureus (inhibition zone was 22.21 mm), E. coli (inhibition zone was 26.26 mm) and P. aeruginosa (inhibition zone was 27.33 mm), as well as fungi, namely C. albicans (inhibition zone was 28.25 mm) and A. fumigatus (inhibition zone was 8.5 mm). Meanwhile, A. alternata and A. niger exhibited lower antibacterial activity than P. rubens. At the same time, A. alternata and A. niger did not show any antifungal activity against C. albicans and A. fumigatus. Several studies have been performed to explore new antimicrobial compounds derived from endophytic fungi. Bezerra et al. [6] tested the antimicrobial activity of some isolates of endophytic fungi, and they found that 34.3% of the fungal isolates exhibited activity, particularly P. glabrum, P. commune, Aspergillus ochraceus and Gibberella baccata. The MIC of P. rubens filtrate extract only was evaluated against tested organisms, with MIC 19.50 µg/mL, 22.30 µg/mL, 21.62 µg/mL, 19.70 µg/mL, 31.2 µg/mL and 39.50 µg/mL for B. subtilis, S. aureus, E. coli, P. aeruginosa C. albicans and A. fumigatus, respectively (Table 4). Methyl stearate was among the detected compounds in P. rubens filtrate extract; according to a previous study [37], it possesses antifungal activity. Moreover, in agreement with Davoodbasha et al. (2018), Gr + ve and Gr − ve bacteria were inhibited by hexadecanoic acid methyl ester, which was detected in the filtrate extract of P. rubens. Some compounds-for example, N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide and 1,2-benzenedicarboxylic acid, were included in the filtrate extracts of P. rubens, A. alternata and A. niger; the antimicrobial activity may due to the presence of such compounds. Our results are in line with the findings of Basheer [41], who found that most of the endophytic fungi isolated from A. marina (red sea in Egypt) had antimicrobial activity but with different levels of microbial growth inhibition. Moreover, from Malaysian mangrove Rhizophora mucronata, various endophytic fungi were isolated and exhibited bactericidal effects against E. coli, P. aeruginosa, S. aureus and B. subtilis [8]. In another study, extracts of marine-derived Turkish fungi such as A. alternata and A. niger exhibited antimicrobial activity against S. aureus, S. epidermidis, B. subtilis, E. coli, P. aeruginosa, Klebsiella pneumoniae, C. albicans and C. parapsilosis, but P. rubens exhibited low and narrow-spectrum antimicrobial activity against P. aeruginosa, B. subtilis and E. coli only [42].

Cytotoxicity of Fungal Extract
The cytotoxicity of P. rubens, A. alternata and A. niger extracts was evaluated against normal cells of human lung fibroblasts (WI-38 cell line) (Figure 8). The results indicated that P. rubens extract showed negligible cytotoxicity at concentrations up to 100 µg/mL and weak cytotoxicity at concentrations up to 400 µg/mL and reached 9.09% mortality, suggesting the possibility of further anticancer activity, as well as other pharmaceutical applications. On the other hand, the cytotoxicity of A. alternata and A. niger extracts was more evident than that of P. rubens extract, particularly at 400 µg/mL.

Cytotoxicity of Fungal Extract
The cytotoxicity of P. rubens, A. alternata and A. niger extracts was evaluated against normal cells of human lung fibroblasts (WI-38 cell line) (Figure 8). The results indicated that P. rubens extract showed negligible cytotoxicity at concentrations up to 100 µg/mL and weak cytotoxicity at concentrations up to 400 µg/mL and reached 9.09% mortality, suggesting the possibility of further anticancer activity, as well as other pharmaceutical applications. On the other hand, the cytotoxicity of A. alternata and A. niger extracts was more evident than that of P. rubens extract, particularly at 400 µg/mL. Therefore, the extract of P. rubens was tested against prostate cancer (PC-3) proliferation. The anticancer activity test showed that P. rubens extract exhibited inhibitory activity towards PC-3 proliferation in a dose-dependent manner (Figure 9), where the cell viability decreased with the increase in the extract concentration. At 25 µg/mL, cell viability was 50.60%, while at 200 µg/mL and 400 µg/mL, the cell viability was 24.09% and 23.80%, reflecting mortality levels of 75.91% and 76.2%, respectively. Hence, the obtained findings also indicated that the endophytic fungus P. rubens, of marine habitats, has acceptable inhibitory activity for PC-3. A. niger and P. rubens displayed the highest anticancer activity against a colon cancer cell line (HCT-116) [42]. In a previous study, inhibition of HL-60 and A-549 cell line proliferation may have been to chloctanspirone A, as identified by Bladt et al. [43] in P. rubens extract. normal cells of human lung fibroblasts (WI-38 cell line) (Figure 8). The results indicated that P. rubens extract showed negligible cytotoxicity at concentrations up to 100 µg/mL and weak cytotoxicity at concentrations up to 400 µg/mL and reached 9.09% mortality, suggesting the possibility of further anticancer activity, as well as other pharmaceutical applications. On the other hand, the cytotoxicity of A. alternata and A. niger extracts was more evident than that of P. rubens extract, particularly at 400 µg/mL.   Therefore, the extract of P. rubens was tested against prostate cancer (PC-3) proliferation. The anticancer activity test showed that P. rubens extract exhibited inhibitory activity towards PC-3 proliferation in a dose-dependent manner (Figure 9), where the cell viability decreased with the increase in the extract concentration. At 25 µg/mL, cell viability was 50.60%, while at 200 µg/mL and 400 µg/mL, the cell viability was 24.09% and 23.80%, reflecting mortality levels of 75.91% and 76.2%, respectively. Hence, the obtained findings also indicated that the endophytic fungus P. rubens, of marine habitats, has acceptable inhibitory activity for PC-3. A. niger and P. rubens displayed the highest anticancer activity against a colon cancer cell line (HCT-116) [42]. In a previous study, inhibition of HL-60 and A-549 cell line proliferation may have been to chloctanspirone A, as identified by Bladt et al. [43] in P. rubens extract.

Molecular Docking Studies
N-(4,6-Dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide and 1,2-benzenedicarboxylic acid, as detected in the filtrate extract of P. rubens, were docked against the crystal structure of C. albicans (PDB = 6TZ6) and the cryo-EM structure of B. subtilis (PDB = 7CKQ) to elucidate the antimicrobial activity of the fungal filtrate extract. We noticed that N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide had the lowest binding energy score with both 6TZ6 and 7CKQ proteins revealing the highest activity at −6.04905 kcal/mol and −6.590 kcal/mol, respectively. On the contrary, 1,2-benzenedicarboxylic acid showed docking scores of −4.74927 kcal/mol and −3.97767 kcal/mol for 6TZ6 and 7CKQ, respectively. The ranking poses generated by the scoring functions are given in Tables 5 and 6. A list of hydrogen bonds between compounds with coenzymes of the chosen proteins is, respectively, presented in Tables 7 and 8. Figures 10 and 11 show, respectively, the best-fitting poses adopted by the compounds docked into proteins 6TZ6 and 7CKQ. In the case of N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide, we observed an interaction with 6TZ6 Meanwhile, 7CKQ interacted with 1,2-benzenedicarboxylic acid through hydrogen bonding between two oxygen atoms of the compound towards the GLN 159 and LYS 155 amino acid residues. In the current study, N-(4,6-dimethyl-2-pyrimidinyl)-4-(4-nitrobenzylideneamino) benzenesulfonamide and 1,2-benzenedicarboxylic acid showed acceptable RMSD (the root mean square deviation of the pose, in Å, from the original ligand) values. The biological activity of a number of natural compounds has been documented with molecular docking studies-for example, the efficacy of chlorogenic acid on human coronavirus (HCoV 229E) and Proteus vulgaris [31]; Aloe vera gel based on chitosan nanoparticles against Helicobacter pylori [44]; and the efficacy of neophytadiene, luteolin, chrysoeriol and kaempferol against P. aeruginosa, E. coli, human prostate and breast-cancer-associated proteins, respectively [45].   [31]; Aloe vera gel based on chitosan nanoparticles against Helicobacter pylori [44]; and the

Conclusions
A. marina leaves are rich in different and various endophytic fungi. P. rubens exhibited good antimicrobial and anticancer activities compared with the other isolates. The extract of P. rubens was more effective than other fungal extracts against the tested microorganisms due to the presence of a number of compounds that were detected in GC-MS analysis. Using molecular docking modeling, we discovered the effectiveness of the studied compounds against C. albicans and B. subtilis, which could motivate the design and synthesis of more antibacterial agents. Finally, the current article may help in our understanding of the fungal diversity of a number of marine environments with diverse biological utilities.