Therapeutic Potentials of Syzygium fruticosum Fruit (Seed) Reflected into an Array of Pharmacological Assays and Prospective Receptors-Mediated Pathways

Syzygium fruticosum (SF), a valuable Bangladeshi fruit, is considered an alternative therapeutic agent. Mainly, seeds are used as nutritional phytotherapy to ease physical and mental status by preventing chronic diseases. Here, we scrutinized the S. fruticosum seed’s fundamental importance in traditional medicine by following an integrated approach combining in vivo, in vitro, and in silico studies. The SF was fractionated with different solvents, and the ethyl acetate fraction of SF (EaF-SF) was further studied. Mice treated with EaF-SF (200 and 400 mg/kg) manifested anxiolysis evidenced by higher exploration in elevated plus maze and hole board tests. Similarly, a dose-dependent drop of immobility time in a forced swimming test ensured significant anti-depressant activity. Moreover, higher dose treatment exposed reduced exploratory behaviour resembling decreased movement and prolonged sleeping latency with a quick onset of sleep during the open field and thiopental-induced sleeping tests, respectively. In parallel, EaF-SF significantly (p < 0.001) and dose-dependently suppressed acetic acid and formalin-induced pain in mice. Also, a noteworthy anti-inflammatory activity and a substantial (p < 0.01) clot lysis activity (thrombolytic) was observed. Gas chromatography-mass spectrometry (GC–MS) analysis resulted in 49 bioactive compounds. Among them, 12 bioactive compounds with Lipinski’s rule and safety confirmation showed strong binding affinity (molecular docking) against the receptors of each model used. To conclude, the S. fruticosum seed is a prospective source of health-promoting effects that can be an excellent candidate for preventing degenerative diseases.


Introduction
Since primitive times, nutraceuticals have offered additional health benefits to human beings for curing different diseases [1]. Hence, people have become more dependent on species, natural products, and nutritional phytotherapy because they possess many novel

Collection and Preparation of Extract
The SF was collected, with permission, during August 2018, from Kaptai hill tracts (22°29'26.99" N 92°13'27.00" E), Rangamati district, Chittagong, Bangladesh. It was authenticated by a taxonomistand deposited at the National Herbarium, Dhaka, Bangladesh under the accession number: 1326. The seeds were subjected todrying under shadow for fifteen days. Later, the dried seedswere ground toa coarse powder by a grinder, and the fine powders (450 g) were soaked in 1.5 L of methanol for 7 days with shaking and stirring. Afterwards, filtration was undertaken by a Whatman No. 1 filter paper, while evaporation was attained at 55 °C by usingawater bath, and concentrated samples were stored in an amber glass vial. Additionally, the extract was then subjected to fractionationby different solvents to obtain petroleum ether fraction (PeF-SF: 8.59 gm), chloroform fraction (CeF-SF: 11.28 gm), ethyl acetate fraction (EaF-SF: 15. 80 gm) and aqueous fraction (AqF-SF: 9.90 gm) following the Kupchan and Tsou protocol [19].

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis
GC-MS analysis of the EaF-SFwas determined using a mass spectrometer (GC-MS TQ 8040, Shimadzu Corporation, Kyoto, Japan) by electron impact ionization (EI) technique on gas chromatography (GC-17A, Shimadzu Corporation, Kyoto, Japan). A fused silica capillary column (Rxi-5 ms; 0.25 m film, 30 m long and internal diameter 0.32 mm) was coated with DB-1 (J&W).The oven temperature was set at 70 °C (0 min); 10 °C, 150 °C (5 min); 12 °C, 200 °C (15 min); 12 °C, 220 °C (5 min) with a hold time of 10 min and the inlet temperature was 260 °C. The helium gas flow rate and pressure in the column were 0.6 mL/min and 90 kPa, respectively. The aux (GC to MS interface) temperature was 280 °C. The MS was set with a scan mode in a range of 40-350 amu, whereas the ionization mode was electron ionization (EI) type, and the mass range was set within 50-550 m/z.

Collection and Preparation of Extract
The SF was collected, with permission, during August 2018, from Kaptai hill tracts (22 • 29 26.99 N 92 • 13 27.00 E), Rangamati district, Chittagong, Bangladesh. It was authenticated by a taxonomist and deposited at the National Herbarium, Dhaka, Bangladesh under the accession number: 1326. The seeds were subjected to drying under shadow for fifteen days. Later, the dried seeds were ground to a coarse powder by a grinder, and the fine powders (450 g) were soaked in 1.5 L of methanol for 7 days with shaking and stirring. Afterwards, filtration was undertaken by a Whatman No. 1 filter paper, while evaporation was attained at 55 • C by using water bath, and concentrated samples were stored in an amber glass vial. Additionally, the extract was then subjected to fractionation by different solvents to obtain petroleum ether fraction (PeF-SF: 8.59 gm), chloroform fraction (CeF-SF: 11.28 gm), ethyl acetate fraction (EaF-SF: 15. 80 gm) and aqueous fraction (AqF-SF: 9.90 gm) following the Kupchan and Tsou protocol [19].

Gas Chromatography-Mass Spectrometry (GC-MS) Analysis
GC-MS analysis of the EaF-SF was determined using a mass spectrometer (GC-MS TQ 8040, Shimadzu Corporation, Kyoto, Japan) by electron impact ionization (EI) technique on gas chromatography (GC-17A, Shimadzu Corporation, Kyoto, Japan). A fused silica capillary column (Rxi-5 ms; 0.25 m film, 30 m long and internal diameter 0.32 mm) was coated with DB-1 (J&W). The oven temperature was set at 70 • C (0 min); 10 • C, 150 • C (5 min); 12 • C, 200 • C (15 min); 12 • C, 220 • C (5 min) with a hold time of 10 min and the inlet temperature was 260 • C. The helium gas flow rate and pressure in the column were 0.6 mL/min and 90 kPa, respectively. The aux (GC to MS interface) temperature was 280 • C. The MS was set with a scan mode in a range of 40-350 amu, whereas the ionization Life 2021, 11, 155 4 of 21 mode was electron ionization (EI) type, and the mass range was set within 50-550 m/z. The sample was then run for GC-MS analysis. One microliter of the sample was split into several types. The GC-MS run for 50 min, and the compounds in the peak areas were identified compared to the database of the GC-MS library version of the National Institute of Standards and Technology (NIST 08-S).

Animals
Either sex of Swiss albino mice (age: 4-5 weeks; weight: 25-30 g) were used in this study and procured from the Jahangir-Nagar University, Savar, Dhaka, Bangladesh. The animals were familiarized with the laboratory condition for 10 days (12 h dark and 12 h light cycle; temperature 25 ± 2 • C) by supplying standard laboratory food and distilled water ad libitum. The research work was conducted under the UK Animals (Scientific Procedures) Act 1986 and associated guidelines [20]. All the experimental protocols were assessed and approved by the Planning and Development (P&D) Committee, Department of Pharmacy, International Islamic University Chittagong, Bangladesh under the reference number of Pharm-P&D-97/07'18.

Chemicals
Diclofenac sodium, fluoxetine, diazepam and thiopental sodium were procured from Square Pharmaceuticals Ltd., Dhaka, Bangladesh. Streptokinase was obtained from Beacon Pharmaceutical Ltd., Mymensingh, Bangladesh. All other chemicals and reagents used for this study were in analytical grade and supplied through the local traders.

Experimental Design
A total of four separated grouped was formed, such as negative control (1% tween 80), positive control (standard drug), and test group I (EaF-SF 200 mg/kg) and test group II (400 mg/kg). Each experiment utilized 24 mice containing 6 mice in each section. Tween 80 (1%, w/w) was orally (p.o) received by the group of negative control (10 mL/kg, body weight), whereas the test groups I and II received the dose of EaF-SF 200 and 400 mg/kg, b.w, p.o, respectively. The positive control or standard drugdiazepam (1 mg/kg, b.w,) was intraperitoneally (i.p) given to the mice used in an elevated plus maze test, hole-board test, open field test, and thiopental sodium-induced sleeping time test, while fluoxetine (10 mg/kg, b.w, i.p) was administered for the forced swim test (FST). The diclofenac sodium (10 mg/kg, b.w, i.p) was used to acetic acid-induced writhing and formalin-induced licking test.

Acute Oral Toxicity Test
Before starting the in vivo experiments, the toxicity profile of EaF-SF was analyzed by following Organization for Economic Co-operation and Development (OECD) guidelines. In brief, mice (n = 6) of the test groups were treated with a single oral dose (50, 100, 150, 300, 400, 1000, and 2000 mg/kg) of EaF-SF, whereas the control group received 1 % tween 80 (10 mL/kg). After administration of each dose, mice were placed in a polypropylene cage and observed separately, with particular attention for possible unexpected responses such as allergic reactions (skin and eye irritation, itching, and rash) behavioral changes, and mortality over the next 72 h. An elevated plus maze (EPM) apparatus was used for the evaluation of anxiolytic activity. The EPM apparatus was lifted up 40 cm in height from the floor, connected by two open arms and two closed arms with a central square [21]. The grouped animals were treated as described in Section 2.4 (experimental design section). After 60 min of the treatment, each treated mouse was placed in the mid-point of the EPM, facing towards the The hole-board test (HBT) apparatus comprised a wooden box (40 × 40 × 25 cm) having sixteen evenly distributed holes (each 3 cm in diameter) on the surface of the box, which was lifted from the floor at an elevation of 25 cm. The separated animals were treated as described in Section 2.4 (experimental design section). Thirty min after the treatment, each treated mouse was placed in the middle square of the apparatus and we recorded the head dipping and time for alatency of 1st head dipping during a 5 min period [22].

Antidepressant Test Forced Swim Test (FST)
The forced swim test (FST) for the antidepressant-like activity of EaF-SF in mice was evaluated by the previously described protocol of Adnan et al., 2020 [23]. The animals were treated as described in Section 2.4 (experimental design section) and 60 min after the administration, and mice were forcefully placed to swim in a transparent plastic apparatus (25 × 15 × 25 cm), which was filled with water up to 15 cm (26 ± 1 • C). The immobility time was recorded for 6 min, whereas last for 4 min was considered immobile. The following Equation (2) was followed to calculate the percentage of inhibition of immobility: A is the mean immobility time of the control, and B is the mean immobility time of the test sample.

Locomotor and Sedative Activity
Open Field Test (OFT) The locomotor activity of EaF-SF was evaluated by the open field test (OFT) method, whereas the number of square movements was considered as a behavioral parameter [24]. The apparatus comprised the square box (60 × 60 × 60 cm) with 25 squares of evenly distributed squares (5 × 5 cm), which were marked consecutively as black and white. Each group's allocated animals were treated as described in Section 2.4 (experimental design section). Each group of mice was placed in the mid-point of the apparatus (30 min after administration of doses) and we counted the number of square movements for 3 min over 0, 30, 60, 90, and 120 min sessions.

Sedative Activity Thiopental Sodium-Induced Sleeping Time Test
The sedative activity of the EaF-SF was assessed by the previously described protocol of Uddin et al., 2018 [25]. The animals of each group were treated as described in Section 2.4 (experimental design section). After 20 min, each divided mouse was induced thiopental sodium (40 mg/kg, i.p), which allowed them to sleep. The animals were observed for the onset of sleeping and duration of sleep (Equation (3)).

% effect =
Average duration of sleep for test group Average duration of sleep for control group × 100 2.11. Antinociceptive Activity 2.11.1. Acetic Acid-Induced Writhing Test The antinociceptive activity of the EaF-SF was assessed by an acetic acid-induced pain model in Swiss albino mice [26]. In this method, randomly grouped mice were treated Life 2021, 11, 155 6 of 21 as described in Section 2.4 (experimental design section). After 30 min of the treatment, each group of mice was intraperitoneally (i.p) injected with acetic acid solution (0.7% v/v) in order to induce pain. The number of writhes of each mouse was recorded for 20 min. The following Equation (4) was used to calculate the writhing inhibition (%): A is the average number of writhing's of the negative control and B is the average number of writhing's of the test group/positive control.

Formalin-Induced Licking Test
Formalin-induced analgesia for EaF-SF was measured by the previously described protocol of Auniq et al., 2019 [27]. The dosing of each group was followed, as described in Section 2.4 (experimental design section). After 1 h of the treatment, 20 µL of formalin solution (2.5%, v/v) was injected into the sub-plantar area of the mice's right hind paw. The analgesia of the mice was measured through the licking and biting of the right paw. The response was recorded for the first 5 min which was noted as early phase (0-5 min), and the last 15 min was the late phase (15-30 min) after formalin injection.

Membrane Stabilization Method
Membrane stabilization of human red blood cell for anti-inflammatory activity was assessed by the previously described method of Ansari et al., 2017 [28], which was followed in triplicate manner. The following Equation (5) assessed the percentage inhibition of hemolysis: A = absorbance of control and B = absorbance of the test sample.

Protein Denaturation Assay
The anti-inflammatory activity of EaF-SF was evaluated according to an earlier reported method with minor modifications [28], which was followed in triplicate manner. The following Equation (6)

In Vitro Thrombolytic Activity
The in vitro clot lysis study of human blood was assessed by the method of Prasad et al., 2006 [29]. Six healthy human volunteers (n = 6) were used in the study, and 5 mL blood was withdrawn from the right-hand vein. Moreover, healthy volunteers' selection criteria were based on non-smoking; non-alcoholic with no history of cardiovascular or anticoagulant drug use. From the withdrawn blood, 0.5 mL was immediately distributed in the earlier weighed Eppendorf, which was then subjected to the incubation for 45 min (37 • C) to form clots. Following 45 min of clot formation, the serum was wholly removed from the Eppendorf deprived of any clot deformation. Each Eppendorf was again weighed to assess the clot weight. Later, 100 µL of ethyl acetate fraction extract (10 mg/mL), water (negative control), and streptokinase (positive control) were added, respectively, to each Eppendorf comprising a pre-weighed clot. After that, all the Eppendorf was again subjected to the incubation for 90 min (37 • C). After incubation, the released fluid in the Eppendorf was removed entirely and again the Eppendorf weighed to measure the clot disruption.

ADME/T and Toxicological Properties Analysis
The ADME/T analyses for the twelve compounds' pharmacokinetic properties were measured by Swiss ADME [38] (http://www.swissadme.ch/). Here, the Lipinski rule of five was followed. As per the principles, a compound would be safe for consumption if it satisfies the following criteria: molecular weight (<500 g/mol), hydrogen bond acceptor (<10), hydrogen bond donor (<5), and lipophilicity value (LogP ≤ 5). In addition, toxicological properties were evaluated by an online tool, admetSAR [38], whereas ames toxicity, carcinogenic properties, acute oral toxicity, and rat acute toxicity were followed.

Statistical Analysis
Results are expressed as mean ± standard error of the mean (SEM). * p < 0.05, ** p < 0.01 and *** p < 0.001 were measured as statistically significant while one-way analysis of variance (ANOVA, Dunnett's test) was undertaken using Graph Pad Prism Version 6.0. (San Diego, CA 92108, USA)

Results and Discussion
The exploration of phytochemical as well as pharmacological profiling demonstrated the coexistence of a pool of therapeutically potent phytoconstituents that once again proved the healing capacity of mother nature. Generally, evaluating a medicinal plant requires pre-extraction and extraction steps, which assist in exposing the potent bioactive constituents from medicinal plants. The biomolecules of the plant materials (seeds, leaves, fruits, barks, and flowers) must be preserved before pre-soaking in a suitable solvent. In this regard, proper drying and grinding can influence the preservation of the phytochemicals present in the final extract [39]. To unveil the plant phytochemicals, GC-MS serves as a critical technological platform that enables both the separation and identification of bioactive constituents of amino nitro compounds, alcohols, ester, long-chain hydrocarbon, steroids, and alkaloids. Hence, GC-MS has become a sophisticated means for analyzing low molecular-weight compounds in complex plant extract samples [40]. However, the plant extract's bioactive constituents are very small and volatile, easily de- tected by GC-MS analysis. The special detection system can separate and identify the bioactive constituents from complex, volatile mixtures [41]. In our study, GC-MS of EaF-SF exposed a total of 49 compounds having a retention time between 4.94 and 25.72. The list of the compounds and total ionic chromatogram is shown in Table 1 and Figure 1, respectively. Among 49 compounds, a comprehensive literatures survey revealed 12 potential bioactive compounds, such as guanosine, beta-D-glucopyranose, 1,6-anhydro-, 9-octadecenoic acid (Z)-, phenylmethyl ester, benzoic acid, 3,4,5-trimethoxy-, andrographolide, hexadecanoic acid, methyl ester, pentadecanoic acid, 14-methyl-, methyl ester, eicosanoic acid, phenylmethyl ester, 9,12-octadecadienoic acid, methyl ester, (E,E)-, 3trifluoroacetoxypentadecane, hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester, thymol, and TMS (Trimethylsilyl) derivative.

Anxiolytic Activity
In the mouse model, the EPM and HB are the most frequently used tools, although more open-arms (EPM) entries and increased head dipping (HB) are known to be the anxiolytic activity [18].   Generally, voltage-gated potassium channels are expressed in various mammalian cells, including brain, spinal cord, skeletal and smooth muscles [42]. This transmembrane proteingroup modulates the central and peripheral nervous system's neuronal excitability and is thus responsible for returning the depolarized cell to a resting state [43]. Im- Generally, voltage-gated potassium channels are expressed in various mammalian cells, including the brain, spinal cord, skeletal and smooth muscles [42]. This transmem-brane protein group modulates the central and peripheral nervous system's neuronal excitability and is thus responsible for returning the depolarized cell to a resting state [43]. Importantly, voltage-gated potassium channels open to efflux K + ions leading to depolarization and action potential inhibition by increasing the threshold potential level required for hyperpolarization [44]. In contrast, K + channel blockers cause stress and anxiety by increasing neuronal excitability rendered by cellular failure in achieving resting-state through prolonged action potential, enhanced effective refractory period, and increased axonal conduction [45]. Therefore, voltage-gated potassium channel receptor serves as a perfect therapeutic target for anxiolytic effect. Hence, to further correlate the anxiolytic potentiality (EaF-SF) observed in the EPM test, the selected compounds (12) have interacted with potassium channel receptor (PDB: 4UUJ), portrayed in the docking score ranging from +0.275 to −5.033 kcal/mol ( Table 2). Among all the compounds, guanosine ( Figure 3A) showed the highest docking score (−5.033 kcal/mol) compared to diazepam (standard drug) displayed a docking score (−2.875 kcal/mol). This promising binding affinity also proves the exact anxiolytic insight and mechanism of EaF-SF. Here, 4UUJ: potassium channel receptor (for anxiolytic); 5I6X: human serotonin receptor (for antidepressant); 2OYE and 3HS5: COX-1 and COX-2 receptor (for anti-nociceptive); 4WCU: Phosphodiesterase-4 inhibitor (for anti-inflammatory); and 1A5H: tissue plasminogen activator (for thrombolytic). The bold score indicates the highest score; (-) indicates no score.
In parallel, the dose-dependent elevation if the number of head dipping (HD) andalleviation of latency alongside the reference treatment in the HBT mirrors is considered an anxiolytic property. During the test, the treatment of mice with EaF-SF (200 and 400 mg/kg) revealed an increased HD in a dose-dependent manner ( Figure 2B). Importantly, the dose of 400 mg/kg exposed a significant (p < 0.001) number of HD (42.50 ± 1.93) compare to the control group (26.33 ± 0.88). In addition, the observed latency of HD for 400 mg/kg was 7.50 ± 0.28, while the diazepam showed 3.50 ± 0.64 latency of HD.
During molecular interactions enlisted in Table 2, notably guanosine (−5.033 kcal/mol) compared to diazepam (−2.875 kcal/mol) potentiates gamma-Aminobutyric acid GABA's effect on depression through binding to the receptor. This phenomenon resulted in the conformational change of GABA leading to its affinity to endogenous ligands. It increased psycho-synaptic flux as well as accumulation of the Cl − anion, thus causing hyperpolarization and subsequent reaching of the threshold can exert and anxiolytic effect [46]. According to the previous study, the adoption of HBT is regarded as a good experimental probe to enumerate the anxiolytic properties of EaF-SF [23]. Moreover, the animal's HD activity is inverse and the extent of the latency period is directly proportional to the animal's anxiety state [47]. Our study demonstrates the anxiolytic property of EaF-SF depicted in both in vivo and in silico molecular docking studies. Here, 4UUJ: potassium channel receptor (for anxiolytic); 5I6X: human serotonin receptor (for antidepressant); 2OYE and 3HS5: COX-1 and COX-2 receptor (for anti-nociceptive); 4WCU: Phosphodiesterase-4 inhibitor (for anti-inflammatory); and 1A5H: tissue plasminogen activator (for thrombolytic). The bold score indicates the highest score; (-) indicates no score. In parallel, the dose-dependent elevation if the number of head dipping (HD) andalleviation of latency alongside the reference treatment in the HBT mirrors is considered an anxiolytic property. During the test, the treatment of mice with EaF-SF (200 and 400 mg/kg) revealed an increased HD in a dose-dependent manner (Figure 2 B).Importantly, the dose of 400 mg/kg exposed a significant (p < 0.001) numberof HD (42.50 ± 1.93) compare to the control group (26.33 ± 0.88). In addition, the observed latency of HD for 400 mg/kg was 7.50 ± 0.28, while the diazepam showed 3.50 ± 0.64latency of HD.
During molecular interactions enlisted in Table 2, notably guanosine (−5.033 kcal/mol) compared to diazepam (−2.875 kcal/mol) potentiates gamma-Aminobutyric acid GABA's effect on depression through binding to the receptor. This phenomenon resulted inthe conformational change of GABA leading to its affinity to endogenous ligands. It

Antidepressant Activity
The antidepressant potency of EaF-SF was measured by the FST, where notable antidepressant activity was correlated to the extensive inhibition of immobility of 29.06% for the 400 mg/kg dose. Notwithstanding this, statistically significant (p < 0.001), 70.47% inhibition of immobility time was recorded for positive control fluoxetine (10 mg/kg) compared to the negative control ( Figure 2C). On the brighter side, the FST extensively evaluates all classes of antidepressant activity ranging from monoamine oxidase inhibitors (MAOs), selective serotonin reuptake inhibitors (SSRIs), through TCAs [48].
In contrast, the 5-HT receptor family's involvement in the depressive disorder is a well-established fact [49]. Serotonin 1A (5-HT1A) and 1B (5-HT1B) receptors are among the most studied for the implication of depression in humans [50]. In comparison, the same subtypes of serotonin receptors have been found responsible for depressive disorder in mice [51]. Therefore, our insilico molecular docking target on human serotonin receptor protein (PDB: 5I6X) is a well-studied target harboring central and allosteric binding sites for antidepressant activity. This serotonin transporter down regulates serotonergic signal transduction via sodium-and chloride-dependent neurotransmitter reuptake into the presynaptic neurons [32]. In our study, "andrographolide" displayed ( Figure 3B) marked interaction (−6.905 kcal/mol) contrasting the standard drug "fluoxetine" (−8.576 kcal/mol) with human serotonin receptor (Table 2), at via hydrogen bonding with ASP-98, GLU-493 and TYR-95 residues and hydrophobic interaction with PHE-341, VAL-501, PHE335 and ILE-172 residues (Table 3). Therefore, complying with the previous finding regarding serotonin receptor activation, we propose a partial binding at the active (competitive agonist) site or an allosteric binding can be a reason behind this limited yet considerable antidepressant activity of EaF-SF which illuminates the parallel anxiolytic-antidepressant potential as relevant to the forced swimming test (FST) performed by the subject animals.  Here, 4UUJ: potassium channel receptor (for anxiolytic); 5I6X: human serotonin receptor (for antidepressant); 1A5H: tissue plasminogen activator (for thrombolytic); 4WCU: Phosphodiesterase-4 inhibitor (for antiinflammatory); and 2OYE and 3HS5: COX-1 and COX-2 receptor (for anti-nociceptive).

Locomotor and Sedative Activity
We further extended our study in the locomotive (open field test) potential of EaF-SF (200 and 400 mg/kg) on mice, and observed reduced exploratory behavior resembling decreased movement (68.45 ± 5.04 in 0 min), (44.48 Figure 2D). Along with prolonged sleeping latency, there was a quick onset of sleep induction in the thiopental sodium-induced sleeping test (Table 4) referring to the locomotive dormancy development of a common anxiolytic-sedative effect to diazepam (positive control). However, the recorded duration of sleeping for 200 and 400 mg/kg EaF-SF was, respectively, 40.0 ± 1.47 and 46.0 ± 1.77, compared to the negative control group (44.11 ± 2.13), whereas the duration of sleeping for thiopental sodium (positive control) was 143.4 ± 13.19 (p < 0.001). Thiopental sodium belongs to the thiobarbiturate group, which interacts with GABA receptors and exposes GABA mediated hyperpolarization in postsynaptic neurons [32]. It also boosts GABA action, and can potentially block excitatory glutamate receptors, thus reducing neuronal response [23]. However, current observation opened the avenue of understanding EaF-SF-plausible preferred pathway of anxiolytic and sedative activity with marked locomotive dormancy along with limited concomitant antidepressant-like effects. Values are represented as Mean ± SEM (n = 6). ** p < 0.01, *** p < 0.001 are statistically significant in comparison to Tween-80 (Control: 10 mL/kg) followed by one-way ANOVA (Dunnett's test). Here, EaF-SF: Ethyl acetate fraction of Syzygiumfruticosum; Reference drug: diazepam (1 mg/kg).

Anti-Nociceptive Activity
On the other part of this study, we enumerated an impressive extent of anti-nociceptive properties reflected in the acetic acid-induced writhing test and formalin-induced licking test. The acetic acid-induced writhing test is a well-recognized protocol to explore peripherally reactive analgesics, whereas the formalin-induced licking test provides a consistent concept of whether the extract's effects are mediated by central or peripheral processes [52,53].
However, in the acetic-acid induced writhing inhibition test, EaF-SF at the doses of 200 and 400 mg/kg revealed an extremely significant (p < 0.001) reduction in the number of writhing 34.75 ± 0.85 and 24.75 ± 0.62, with 24.86% and 46.49% writhing inhibition, respectively compared to the negative control (46.25 ± 1.54). Furthermore, the positive control exposed an extremely significant (p < 0.001) reduction in analgesia (64.86%) (Figure 4). ing test. The acetic acid-induced writhing test is a well-recognized protocol to explore peripherally reactive analgesics, whereas the formalin-induced licking test provides a consistent concept of whether the extract's effects are mediated by central or peripheral processes [52,53].
However, in the acetic-acid induced writhing inhibition test, EaF-SF at the doses of 200 and 400 mg/kg revealed an extremely significant (p < 0.001) reduction in the number of writhing 34.75 ± 0.85 and 24.75 ± 0.62, with 24.86% and 46.49% writhing inhibition, respectively compared to the negative control (46.25 ± 1.54). Furthermore, the positive control exposed an extremely significant (p < 0.001) reduction in analgesia (64.86%) ( Figure  4). The inhibition of neurogenic (early phase) and inflammatory pain (late phase) by EaF-SF (200 and 400 mg/kg) is presented in Table 5. In the early phase, EaF-SF at the doses of 200 and 400 mg/kg inhibited 14.75% and 38.96% pain response induced by the formalin. While in the late phase, EaF-SF markedly decreased the pain sensation by 23.07% and 54.93%, the positive control diclofenac sodium revealed a significant decrease in nociception in both phases by 48.41% and 68.05%, respectively.  Values are represented as mean ± standard error of the mean (SEM, n = 6). *** p < 0.001 is statistically significant in comparison to Tween-80 (Control: 10 mL/kg) followed by one-way ANOVA (Dunnett's test). Here, EaF-SF: Ethyl acetate fraction of Syzygiumfruticosum; Reference drug: diclofenac sodium (10 mg/kg).
The inhibition of neurogenic (early phase) and inflammatory pain (late phase) by EaF-SF (200 and 400 mg/kg) is presented in Table 5. In the early phase, EaF-SF at the doses of 200 and 400 mg/kg inhibited 14.75% and 38.96% pain response induced by the formalin. While in the late phase, EaF-SF markedly decreased the pain sensation by 23.07% and 54.93%, the positive control diclofenac sodium revealed a significant decrease in nociception in both phases by 48.41% and 68.05%, respectively. In an acetic acid-induced abdominal writhing model, acetic acid acts indirectly by inducing the release of endogenous mediators which stimulate the nociceptive neurons that are sensitive to non-steroidal anti-inflammatory drugs, narcotics, and other centrally active drugs [54]. On the other hand, formalin stimulates sensory neurons by directly activating a substantial inflammatory pain mediating cation channel TRPA1. Formalin induces pain in two phases. The first phase directly activates primary afferent sensory neurons then leads to combined effects of afferent input and central sensitization in the dorsal horn in the second phase [26]. In both models, EaF-SF demonstrated striking results. However, to understand molecular pathway of analgesic response by EaF-SF's, we carried out an insilico molecular docking study of 12 selected compounds with COX1 and COX2. The inflammatory cell-induced COX2 inhibition is one of the primary analgesia pathways alongside the peripheral prostaglandin synthesis enzyme inhibition; whereas, the ratio of COX1 and COX2 has been proposed to be the reason to determine the extent of adverse effects of non-steroidal anti-inflammatory drugs (NSAID). The interaction of 12 compounds with COX-1 enzyme (PDB: 2OYE) (interaction scores ranging from −1.676 to −7.16 kcal/mol, where 3-trifluoroacetoxypentadecane displayed the maximum interaction ( Figure 3C)) and COX-2 enzyme (PDB: 3HS5) (guanosine demonstrated the highest docking score of −7.495 kcal/mol ( Figure 3D) is summarized in Table 2. Importantly, it is also conceived that NSAIDs exert an antinociceptive response centrally, which is served either by individual effect or coordination of prostaglandin synthesis interference within CNS, endogenous opioid peptides and blockade of the serotonin (5-hydroxytryptamine) release [55]. The relevance of this assumption has feasibility based on the serotonergic involvement of andrographolide compound detected in EaF-SF. Moreover, several phytomedicinal compounds families including flavonoids, phenolics, alkaloids, lipids, terpenes, and phthalides, have demonstrated neuroprotective potential by governing the blood-brain barrier (BBB) breakdown associated signaling transduction [56,57]. Similarly, for the central antinociceptive pathway of mediation andrographolide as well as highest COX1 and COX2 interaction displaying trifluoroacetoxypentadecane and guanosine needed to cross BBB [58].
However, for the anti-inflammatory docking study, andrographolide also exposed the highest binding interaction against the PDE4 receptor (PDB: 4WCU) with a docking score of −7.931 kcal/mol ( Figure 3E). In this study, diclofenac sodium and aspirin were used as the standard drug, whereas the aspirin demonstrated a docking score of −5.671 kcal/mol ( Table 2).

In Vitro Thrombolytic Activity
In a thrombus, plasminogen is triggered by the activation of platelets trapped fibrin mesh leading to zymogen-plasminogen cleavage at the Arg561-Val562 peptide bond, causing the formation of plasmin and subsequent fibrinolysis [59,60]. In our thrombolytic experiment, positive control streptokinase (1,500,000 I.U.) demonstrated a significant (p < 0.001) percentage (75.35 ± 2.4%) of the clot lysis compared to the negative control (12.31 ± 1.5%). Furthermore, the EaF-SF revealed 32.75 ± 5.10% clot lysis activity with a significance value of p < 0.01 ( Figure 5C). However, to figure out the molecular aspect of our experiment, we carried out the thrombolytic docking study. The docking of 12 compounds against the tissue plasminogen activator receptor (PDB: 1A5H) revealed the interaction scores ranging from −0.149 to −7.885 kcal/mol, whereas the Guanosine exhibited the highest interaction ( Figure 3F). Here, the standard drug streptokinase demonstrated a docking score of −5.704 kcal/mol. However, for the anti-inflammatory docking study, andrographolide also exposed the highest binding interaction against the PDE4 receptor (PDB: 4WCU) with a docking score of −7.931 kcal/mol ( Figure 3E). In this study, diclofenac sodium and aspirin were used as the standard drug, whereas the aspirin demonstrated a docking score of −5.671 kcal/mol (Table 2). . ** p < 0.01 and *** p < 0.001 are statistically significant followed by one-way ANOVA (Dunnett's test). Here, EaF-SF: Ethyl acetate fraction of Syzygium fruticosum; Reference drug: aspirin, diclofenac sodium, and streptokinase.

In Vitro Thrombolytic Activity
In a thrombus, plasminogen is triggered by the activation of platelets trapped fibrin mesh leading to zymogen-plasminogen cleavage at the Arg561-Val562 peptide bond, causing the formation of plasmin and subsequent fibrinolysis [59,60]. In our thrombolytic experiment, positive control streptokinase (1,500,000 I.U.) demonstrated a significant (p < 0.001) percentage (75.35 ± 2.4%) of the clot lysis compared to the negative control (12.31 ± 1.5%). Furthermore, the EaF-SF revealed 32.75 ± 5.10% clot lysis activity with a significance value of p < 0.01 ( Figure 5C). However, to figure out the molecular aspect of our experiment, we carried out the thrombolytic docking study. The docking of 12 compounds against the tissue plasminogen activator receptor (PDB: 1A5H) revealed the interaction scores ranging from −0.149 to −7.885 kcal/mol, whereas the Guanosine exhibited the highest interaction ( Figure 3F). Here, the standard drug streptokinase demonstrated a docking score of −5.704 kcal/mol. . ** p < 0.01 and *** p < 0.001 are statistically significant followed by one-way ANOVA (Dunnett's test). Here, EaF-SF: Ethyl acetate fraction of Syzygium fruticosum; Reference drug: aspirin, diclofenac sodium, and streptokinase.

ADME/T and Toxicological Properties Analysis
From the toxicological standpoint, our ADME/T analysis incriminates the toxicokineticintervention points of the subjected 12 phytogenic compounds of EaF-SF with Lipinski's rule of five (Table 6). Lipinski's ruleof five demonstrates the prospective extent of drugs absorption and bioavailability upon the range of Log P, Molecular weight, and hydrogen bond donor/acceptor properties [41]. According to Lipinski'srule of five, an orally active drug would not violate more than one of the base parameters, which most of the selected compounds comply with. In addition, the toxicological properties of 12 selected compounds of EaF-SF also revealed non-Ames toxic, non-carcinogenic properties of the compounds along with a subtle acute toxicity in mice (Table 7). Most importantly, during an acute oral toxicity test (treated dose ranging from 50 to 2000 mg/kg), mice did not expose any evidence of physiological and behavioral abnormalities. Moreover, no mortality or any other toxicity were observed for 72 h after oral administration of EaF-SF, which confirmed that EaF-SF has no toxic profile up to 2000 mg/kg (data not shown).

Conclusions
The present study on EaF-SF demonstrated significant anxiolytic and antidepressant and sedative activity. The study also exhibited an antinociceptive activity which was evident by various analgesia models. Additionally, the information obtained on the denaturation of protein, hemolysis, and clot lysis supports the anti-inflammatory and thrombolytic activities of EaF-SF. The potential compounds identified by GC-MS displayed an outstanding assortment of pharmacological properties enumerated by the in silico molecular docking studies. Our results revealed the parallel anxiolytic-antidepressant activity owing to voltage-gated potassium channel interaction and serotonin receptor activation, respectively. The lead compounds of EaF-SF competitively bind with the serotonin receptor in allosteric sites causing alleviation of exploratory behaviour along prolonged sleeping latency, indicating locomotive dormancy development. We also enumerated an impressive extent of antinociceptive and anti-inflammatory activity attained by different molecules' combined interaction with COX-1 and COX-2 enzymes confirmed by invitro membrane stabilization as well as insilico receptor interaction. Moreover, EaF-SF demonstrated striking thrombolytic activity with plausible inhibition of tissue plasminogen activators. Further wet-lab molecular analysis can illuminate the avenue of new therapeutic findings involving some or most of these compounds underlying functionality. Overall, Syzygium fruticosum is a potential source of various health-promoting bioactive compounds that can be used as a nutritional phytotherapy to prevent various degenerative diseases.

Data Availability Statement:
The data presented in this study are available on reasonable request from the corresponding author.